Student Name: Teacher: Date: District: Robeson Assessment: 9_12 Agriculture AU71 - Biotech and Agrisci Rsch I Test 3

Transcription

1 Student Name: Teacher: Date: District: Robeson Assessment: 9_12 Agriculture AU71 - Biotech and Agrisci Rsch I Test 3 Description: Objective Lab Safety & Equipment Form: Micropipettes are used by agricultural researchers to: A. Extract blood and tissue from biological materials. B. Inject bacteria and microorganisms dermally into animal research subjects. C. Measure and extract small amounts of liquids for use in laboratory experiments. D. Measure and extract small amounts of solids for use in laboratory experiments. 2. Laboratory fires can be especially dangerous because of the presence of extremely volatile chemicals and flammable liquids. Safely extinguishing these fires requires the use of: NCCTE.9_12.AE.AU71.BA09.04 A. A fire blanket. B. Class A fire extinguishers. C. Class B or Class D fire extinguishers. D. Large amounts of water. 3. Which is the LEAST important reason for using specialized chemical cabinet? A. Contain and smother fires B. Close easily and seal tightly C. Isolate chemicals that might leak D. Store report forms

2 4. Melissa is logging the results of a series of experiments on the germination rates of genetically modified rice seed after various pretreatments. What software program will allow her to BEST enter and manipulate both quantitative and qualitative data? NCCTE.9_12.AE.AU71.BA10.03 A. A database program (Microsoft Excel) B. A graphic editing program (Macromedia Fireworks) C. A presentation program (Microsoft PowerPoint) D. A word processing program (Microsoft Word) 5. Special care should be taken to prevent the mixing of chemicals during disposal in a sink as some chemicals used in agricultural labs: A. Can eat through drain pipes. B. Occur in granular or powdered form. C. Produce noxious fumes. D. React violently when combined. 6. Knowing the concentration of chemicals used in laboratory experiments is critical as: NCCTE.9_12.AE.AU71.BA09.03 A. Less concentrated chemicals could lead to lab failure or unexpected results. B. More concentrated chemicals will achieve better results. C. More concentrated chemicals will help to speed up any results achieved in a lab. D. Pure chemicals achieve more effective results. 7. Keely is finalizing her research study. She uses Microsoft PowerPoint, Apple Keynote, and other presentation software programs to: NCCTE.9_12.AE.AU71.BA10.03 A. Convey research findings to other researchers and the general public. B. Create websites detailing research findings. C. Edit images taken of the research process or research results. D. Keep accurate records of research findings.

3 8. The use of a laboratory coat is: A. A good method to prevent contamination of research. B. Constricting during laboratory work. C. Not necessary for experiments conducted in agricultural laboratories. D. Only useful for protecting clothing underneath. 9. Prior to using chemicals in agricultural laboratory experiments, individuals must have read and know the current location of the: NCCTE.9_12.AE.AU71.BA09.03 A. Environmental Protection Agency Warning (EPA-W). B. Manufacturer s Promotion Sheets (MPS). C. Material Safety Data Sheet (MSDS) and container label. D. United States Department of Agriculture Annual Report (USDAR). 10. When using aseptic procedure in a laboratory, instruments should be disinfected: A. After the lab. B. At the beginning of the lab. C. At the end of the day. D. Before each new step in the lab. 11. Researchers can NOT use a word processing program in the construction of a final summary on a research project to: NCCTE.9_12.AE.AU71.BA10.03 A. Create animated images to explain research techniques. B. Hyperlink text to results published on the internet. C. Insert images and charts produced in other programs. D. Type large amounts of text detailing results and conclusions.

4 12. Laminar flow hoods utilize complex filters to remove the smallest microorganisms from air and create an aseptic environment needed for: A. Artificial insemination. B. Bioremediation. C. Plant tissue culture. D. Semen evaluation. 13. Securing the area, notifying others, and immediately rinsing and treating any areas of exposed skin are the steps one should follow in a laboratory after: A. A chemical is spilled. B. Breaking glass lab ware. C. Combining the incorrect ingredients in a solution. D. Inhalation of noxious fumes. 14. Shakers are laboratory instruments used to: A. Encourage the growth of bacteria on culture plates. B. Mix or agitate solutions or compounds. C. Speed the drying of glassware after cleaning. D. Stir solutions at a consistent rate. 15. When utilizing an autoclave, gauges should be checked often and the device should be carefully monitored during use as: NCCTE.9_12.AE.AU71.BA10.02 A. Successful sterilization requires the device to be rotated every 10 minutes. B. The device often requires adjustment during the sterilization process. C. The high pressure used for sterilization can be dangerous. D. The low temperatures used for sterilization can be dangerous.

5 16. What instrument is used in the culturing of bacteria plates to ensure stable temperatures appropriate to the specific microorganism? A. Autoclave B. Incubator C. Lab refrigerator D. Scientific oven 17. Although enzymes and chemicals found in small quantities in food and other naturally occurring materials are usually safe to handle, protection and safety precautions should be used when these same enzymes and chemicals are used in laboratories in: NCCTE.9_12.AE.AU71.BA09.03 A. Diluted form. B. Concentrated form. C. Liquid form. D. Solid form. 18. When using an incubator, Hunter makes certain that bacteria culture plates are: NCCTE.9_12.AE.AU71.BA10.02 A. Covered and the incubator has high levels of humidity. B. Covered and the incubator is sealed. C. Uncovered and the incubator has high levels of humidity. D. Uncovered and the incubator is sealed. 19. The highest fertility rates can be achieved in artificial insemination if frozen semen is removed from cold storage, quickly dried, and placed directly into a water bath set at: NCCTE.9_12.AE.AU71.BA10.02 A. 99 F for around 45 seconds. B. 105 F for around 45 seconds. C. Room temperature and immediately increased to 93 F. D. Room temperature and immediately increased to 105 F.

6 20. When measuring the mass of live organisms, which instruments is the most accurate and effective method: A. Digital scale. B. Graduated cylinder. C. Single beam balance. D. Triple beam balance. 21. What microscope is MOST often used in high school agricultural research labs? A. Compound light microscope B. Digital microscopes C. Scanning electron microscopes D. Tunneling electron microscopes 22. An individual with chemical exposure to his/her eyes should, before seeking medical attention, use an eye rinse station to immediately flush eyes for several: A. Minutes. B. Minutes, pausing to rub eyes every 30 seconds. C. Seconds. D. Seconds, then rub eyes, and flush again. 23. Which is true of maintaining all the equipment and materials needed for conducting a particular experiment on the workspace? NCCTE.9_12.AE.AU71.BA09.04 A. Enables researchers to work more quickly and efficiently B. Is a safety hazard, as materials and equipment can clutter the workspace causing accidents. C. Is helpful for researchers, ensuring that everything needed is available. D. Gives other researchers easy access to materials and equipment when it is needed

7 24. Though laboratories should be well ventilated, pungent and noxious chemicals should be used only: A. For short periods of time. B. Near a window. C. Under functioning fume hoods. D. Under the supervision of a research manager. 25. Which applies direct heat to glassware containing solutions? A. Desiccators B. Hot plates C. Lab microwaves D. Melting point apparatus 26. Mike reads the meniscus, or the lowest part of the curve found at the water level in a graduated cylinder to determine the: NCCTE.9_12.AE.AU71.BA10.02 A. Mass of a liquid. B. Mass of a solid. C. Volume of a liquid. D. Volume of a solid. 27. Micropipettes are used to measure: A. Large quantities of solutions. B. Small quantities of solutions. C. The amount of a gas in solution. D. The temperature of solutions.

8 28. What piece of laboratory equipment spins at rapid rates to condense biological material from liquids in the bottom of small tubes? A. Autoclave B. Centrifuge C. Distiller D. Micropipette 29. Controlled biological materials should be autoclaved after use to ensure: A. Other researchers will not accidentally use the same materials. B. The destruction of any remaining living organisms. C. The materials will survive release into the environment. D. The viability of materials used in research. 30. When maintaining aseptic conditions in a laboratory experiment, contamination of unprotected materials can be prevented during work by: A. Handling material without gloves. B. Placing material on the front edge of the work area. C. Taking a break without washing hands. D. Washing hands and wrists with antibacterial soap. 31. Jennifer is making a solution for a DNA extraction lab that requires the use of a micropipette for accuracy. When using the micropipette she should be careful to make certain that the tip of the pipette: NCCTE.9_12.AE.AU71.BA10.02 A. Has not been recently changed. B. Is completely submerged in the fluid. C. Is slightly loose to allow the insertion of air. D. Is slightly submerged in the fluid.

9 32. Emmanuel is testing for the presence of bacteria on the tea dispensers in a high school cafeteria. Which item will he use to conduct the test? NCCTE.9_12.AE.AU71.BA10.02 A. Centrifuge B. Gas chromatograph C. Gel electrophoresis chamber D. Incubator 33. An instrument used to gradually warm biological materials and maintain constant warm temperature for short periods of time with little or no damage to material is a/an: A. Hot plate. B. Hydrometer. C. Incubator. D. Water bath. 34. For proper laboratory safety, lab workers must know the hazards presented by each chemical and the treatment necessary if exposure occurs. Federal guidelines require that each chemical's information be found on: NCCTE.9_12.AE.AU71.BA09.03 A. Manufacturer s Promotion Sheets. B. The desk of the laboratory manager. C. The Material Safety Data Sheet of the chemical. D. The websites of chemical producers and sellers. 35. What safety item might researchers choose to wear in place of a full face shield when using weak chemicals? A. Contact lenses B. Reading glasses C. Dust mask D. Safety goggles

10 36. Incubation of bacteria cultures should NEVER be conducted at temperatures greater than 30 C as: NCCTE.9_12.AE.AU71.BA09.03 A. 30 C is the ideal temperature for the growth of all bacteria. B. Temperatures lower than this will cause bacteria to reproduce to quickly for researchers to control and monitor. C. Temperatures higher than this will kill most bacteria. D. Temperatures higher than this will promote the growth of bacteria dangerous to human health. 37. The use of restricted biological materials in agricultural laboratories, including many bacteria and microorganisms, requires: A. Proof that organisms will only be used in biotechnology. B. Special permits from the federal government. C. That all organisms be autoclaved prior to use. D. The presence of predator organisms. 38. What is used to sterilize equipment and biological materials and must be monitored carefully because of high internal pressures? A. Autoclave B. Centrifuge C. Incubator D. Laboratory oven 39. All glassware, chemical vessels, and containers in a laboratory should be: A. Disposed of immediately after use. B. Organized by color. C. Properly labeled. D. Unlabeled.

11 40. Which is the LEAST important reason for diluting and/or neutralizing strong chemicals prior to disposal? A. Chemicals containers may lose thier labels B. Federal law states that all chemicals must be diluted before disposal C. High levels of even inane chemicals can cause environmental damage D. Strong chemicals can damage plumbing and sewer systems 41. When diluting acids or creating chemical solutions utilizing concentrated acids, it is important to slowly add the acid into cool water as: NCCTE.9_12.AE.AU71.BA09.03 A. All acids are water soluble. B. Many concentrated acids are not water soluble. C. Many concentrated acids can flash boil water, spattering it out of glassware. D. Many concentrated acids can react violently with water, releasing noxious fumes. 42. Holly reports a dangerous safety condition in the biotech lab. She notes the cause of the problem as: NCCTE.9_12.AE.AU71.BA09.04 A. The placement of flammable chemicals in a locked cabinet. B. The placement of glassware in a rack to dry after cleaning. C. The placement of glassware in cabinets located at eye level. D. The presence of chemical materials with missing or damaged labels. 43. What is NOT an important safety principle for working in laboratory? A. Immediately dispose of biohazards in a proper manner following use B. Know the location and function of emergency washes and safety equipment C. Keep walkways and tables clear of clutter D. Place chemicals and lab ware to the side when not in use

12 44. Wanda spills chemical on her hand. She immediately rinses her hand with water and applies a mildly alkaline chemical to the area. What type of chemical did she spill on her hand to receive the first aid treatment? NCCTE.9_12.AE.AU71.BA09.04 A. Acid B. Base C. Inorganic D. Organic 45. Information on every chemical and solution in a laboratory is MOST likely found in: A. Hazardous material care sheets. B. Lab chemical record sheets. C. Material safety data sheets. D. Material certification records. 46. Marie accidentally swallows a chemical during laboratory procedures. Prior to seeking medical attention, her instructor consults the: NCCTE.9_12.AE.AU71.BA09.04 A. Laboratory safety posters. B. Material safety data sheets for the chemical. C. Technical support from the chemical company. D. The internet. 47. What is NOT an effective method for the sterilization of laboratory equipment and instruments? A. Dipping in a 70% ethanol solution B. Contact with the open flame of a alcohol burner C. Treatment in an autoclave D. Antibacterial soap

13 48. When Marcus pours chemicals or solutions into glassware, he minimizes the risk of accidents by pouring: NCCTE.9_12.AE.AU71.BA09.04 A. Quickly with containers several inches apart. B. Quickly resting one container on the rim of the other, and using a funnel. C. Slowly resting one container on the rim of the other. D. Slowly with containers several inches apart and using a funnel.