Triphenylmethane Dyes in Seafood AOAC Official Method of Analysis

Transcription

1 Triphenylmethane Dyes in Seafood AOAC Official Method of Analysis Wendy C. Andersen U.S. Food and Drug Administration Animal Drugs Research Center Marilyn J. Schneider United States Department of Agriculture Agricultural Research Service - Eastern Reg. Research Ctr. Eric Verdon, Dominique Hurtaud-Pessel, Pierrick Couëdor French Agency for Food, Environment and Occupational Health Safety ANSES - Laboratory of Fougères

2 History AOAC Stakeholders Panel on Marine and Freshwater Foods Quantitative method needed for: Malachite green, leucomalachite green, crystal violet, and leucocrystal violet Shrimp, tilapia, catfish, and salmon 2010 Standard Method Performance Requirements SMPR

3 Veterinary Drug Subgroup Members Identified methods to meet SMPR Recovery = %; Precision = 46 % LOD = 0.5 µg/kg; LOQ = 1.0 µg/kg 2012 ERP Assembled - Analytical Method Selected: Hurtaud-Pessel, Couëdor, Verdon - ANSES J. Chromatogr. A 1218 (2011) 1632 Method used in proficiency testing in European Union

4 Method Principal Extraction of muscle with ACN in the presence of hydroxylamine and magnesium sulfate Extract evaporation and dissolution of analytes in acetonitrile/ascorbic acid Analysis by LC-MS/MS Quantitative analysis with extracted matrix calibrants and four deuterated internal standards to correct for matrix effects and extraction losses

5 2013 ANSES Method First Action GO!!! Oct-Nov: who, what, how, when? Dec-Jan 2014: SLV salmon, catfish, shrimp, tilapia Feb-April: samples shipped and analyzed April Aug: data analysis, statistics

6 US State Labs Mark Crosswhite and Walter Hammack Florida Dept. of Agriculture & Consumer Services Christopher K. Lam and Mary Cumming Tennessee Department of Agriculture Mike Filigenzi and Robyn Noonan CA Animal Health and Food Safety Lab, UC Davis US Federal Labs Alan R. Lightfield and Steven J. Lehotay USDA-ARS Eastern Regional Research Center, PA Sara Lupton USDA-ARS Biosciences Research Laboratory, Fargo, ND Robert Burger FDA/ORA Denver Laboratory Yanxuan Tina Cai FDA/ORA Northeast Regional Laboratory Andrew Fong, John Kibbey, and Chris A. Baker FDA/ORA Arkansas Regional Laboratory Haejung An FDA/ORA Pacific Regional Laboratory Southwest International Labs Dominique Hurtaud-Pessel, Pierrick Couëdor and Eric Verdon ANSES, Laboratory of Fougères, Fougères, France Jason Smith and Ross Potter Canadian Food Inspection Agency, Nova Scotia, Canada Andrew Harris and Linda Lissemore University of Guelph, Laboratory Services, Guelph, Ontario, Canada Private Labs Sarah R. Ruiz and Cheryl D. Stephenson Eurofins Central Analytical Laboratories, LA Anna Hawkins, Shristi Prakash and Michael Conway OMIC USA Inc., Portland OR

7 Study Design 5 analytes (MG, LMG, CV, LCV, and BG) 3 aquaculture types (shrimp, catfish, and salmon) 5 concentration levels blinded samples in duplicate Level of interest is 1.0 µg/kg (ppb) Blank, Spikes (0.42, 0.90, 1.75 µg/kg), Incurred 51 samples per lab FDA Level Four Chemical Method Validation Calibration Extracted extracted matrix calibrants with IS correction 0, 0.25, 0.5, 1.0, 2.5, 5.0 µg/kg d 5 -MG, d 5 -LMG, d 6 -CV, d 6 -LCV

8 Results Summary All 14 labs completed study and reported valid data Compound % Recovery % RSD r Malachite Green Leucomalachite Green Crystal Violet Leucocrystal Violet Brilliant Green HorRat generally <1 Detection Level µg/kg SMPR : Recovery = %; Precision = 46 % LOD = 0.5 µg/kg; LOQ = 1.0 µg/kg Study directors recommend for Final Action

9 More History Dec Results Submitted to Research Institute June J. AOAC International Schneider, Andersen 98 (2015) Andersen, Casey, Schneider, Turnipseed 98 (2015) Method user feedback submitted methodfeedback@aoac.org

10 Since 2015 AOAC Meeting Nov 2015 AOAC ERP teleconference Final Action decision for Feb 2016 OMB - Official Method of Analysis ~Mar 2016 FDA Office of Foods and Veterinary Medicine Chemical Methods Validation Group Designated as Level 4 (Multilab Validation) September 2016 AOAC Method of the Year

11 What s Next? Continuing studies of AOAC Method for aquaculture Matrix effects for unusual samples? Roasted, barbequed, processed seafood samples? Method feedback? wendy.andersen@fda.hhs.gov methodfeedback@aoac.org

12 Acknowledgements Marilyn Schneider, Eric Verdon, Dominique Hurtaud-Pessel, Pierrick Couëdor Christine Casey and Sherri Turnipseed FDA Denver Jo Marie Cook and Joe Boison Collaborative Study Champions! Tom Phillips, John Phillips Statistical Review of Data Study Participants Mark Crosswhite and Walter Hammack Christopher Lam and Mary Cumming Mike Filigenzi and Robyn Noonan Alan Lightfield and Steven Lehotay Sara Lupton Robert Burger Yanxuan Tina Cai Haejung An Andrew Fong, John Kibbey, and Chris Baker Dominique Hurtaud-Pessel, Pierrick Couëdor and Eric Verdon Jason Smith and Ross Potter Andrew Harris and Linda Lissemore Sarah Ruiz and Cheryl Stephenson Anna Hawkins, Shristi Prakash and Michael Conway