Activity Sheet 2 BACTERIA TAKE-DOWN Problem: What metals are the most effective in inhibiting bacterial growth?

Size: px
Start display at page:

Download "Activity Sheet 2 BACTERIA TAKE-DOWN Problem: What metals are the most effective in inhibiting bacterial growth?"

Transcription

1 Activity Sheet 2 BACTERIA TAKE-DOWN Problem: What metals are the most effective in inhibiting bacterial growth? Background: Germs are EVERYWHERE!!! We have hear this all before. Quite often illnesses and diseases can be caused by the transmittance of some sort of microorganism. These different microorganisms could vary from some sort of virus, fungi, or bacteria. These little invaders use the nutrient rich and warm human body to prosper and to replicate themselves (Alberts, Johnson, & Lewis). The surplus of these invaders is often what will cause a person to become ill. Therefore it is the medicines that we take that will play a role in affecting the life and growth of these microorganisms, and in some cases these organisms can even be killed off by the medicines that we take.. Medications can be made of of many things. One ingredient present in many medicines, as weird as it may sound, are different types of metals. Metals such as platinum, titanium, ruthenium, and more have been used in medical applications in the past.some metals have antibacterial properties. In this lab experiment you will be testing to see if any metals seem to have these properties. Hypothesis: What metals are the most effective in inhibiting bacterial growth? I believe that Because, Materials: - petri dishes (atleast 2 per group) - agar powder - heater or incubator - cotton swabs - vinegar - hand sanitizer - marker for labeling - penny, nickel, quarter, aluminum foil, and other miscellaneous metals for testing. - liquid bleach - lab safety gloves Safety: - Wearing lab safety gloves, goggles, and an apron or lab coat - Make sure that bacteria is dead before disposing of it by washing it down the sink. This can be done by using bleach. - Make sure that all bacteria is contained and that hands are always washed after working with samples. - 1

2 Procedure: 1. Aquire 1-2 agar plates. Plate cover should not be removed until instructed to do so. 2. Take the petri dishes out of the refrigerator and turn upside down. Using a marker split your dish up into three different sections. 3. Label each section with the metal that will be placed on the bacteria. Remember that one section should be labeled as controlled or un-treated and another should be labeled as hand sanitizer sample..an example is given to the right. ** Note: The next few steps(4-9) should be done quickly in order to ensure the least amount of contamination** 4. Lif up the lid of the petri dish. 5. Introduce bacteria to the petri dishes. Use your bacteria of choice (ex: a thumb print or swab of the table) in each labeled section of the two petri dishes except for the hand sanitizer section (should be the same source introducing the bacteria into each section of the plate. When introducing bacteria into the dish press your sample down firmly into each section of the dish without breaking the agar. If using a cotton swab make has marks as seen to the right. 6. For the metal sections of the petri dishes add the metal on top of your plated bacteria. NOTE: If you are using coins you should first wash them with vinegar. 7. Quickly re-cover your petri dish. 8. Clean your hands or cotton swab thoroughly with hand sanitizer, let them dry, and then place your sample onto the final section of the perti dish (hand sanitizer sample). 9. After adding your bacteria replace the tops to the appropriate petri dishes quickly, as an extra precaution to prevent contamination, you can place each petri dish in a zipper-lock bag. This will provide an extra layer of protection against any hazardous bacteria colonies that may develop, but will still allow you to view the contents of the petri dish. 10. Record your Day 1 observations on the Data Sheet. 11. Place the petri dishes in a warm, dark place (incubation system is ideal). Leave the petri dishes in a warm, dark place where the bacteria can develop, undisturbed, for several days. The ideal temperature for growing bacteria is around 98 0 F (37 0 C)... Similar to human body temperature!. 12. Leave the petri dishes in their warm dark place for 4-6 days, checking on them each day and writing down observations based of their appearance, smell, and size on the Data Sheet. 13. After the 4-6 days record your final observations and compare your results with the rest of the class and examine which metals seemed to inhibit bacterial growth the most. - 2

3 Data Sheet- Day 1: Day 2: - 3

4 Day 3: Day 4: - 4

5 Day 5: Day 6: - 5

6 Discussion Questions: 1. How extensive was the growth of the control section of your petri dishes? Did this surprise you? a. Explain why you believe you normally do not see this bacteria on your hand? 2. Did your group see that any metals affected the growth of your bacteria compared to your control? Explain. 3. After comparing results with the rest of the class what were some other metals that were effective in killing bacteria in this experiment? Did these groups use a different source for their bacteria than you? Explain your findings. 4. Research the ingredients in pepto bismol and how it works. Write down some of your findings and record if you see any correlations to the lab we have completed today. 5. Explain why do you believe it may be important to use hand sanitizers and antibacterial soaps? 6. Hand sanitizers often claim to kill 99.99% of germs. What do you believe happens to that remaining 0.01% of germs that are not killed? Explain. 7. Explain how do you believe garguling salt water could help a sore throat? 8. Explain why you believe that metals may be used in sportswear. 9. It takes on average 12 years and over US$350 million to get a new drug from the laboratory onto the pharmacy shelf. Explain why you believe this process is so lengthy, considering many drugs have metals in them. - 6

7 Conclusion Questions: 1. What metal was most effective as an antibacterial? 2. What were your findings with the hand sanitized section of the petri dish? 3. How do antibiotics work? 4. Research metals that have shown to hinder bacteria in medicine. 5. Research the drug cisplatin and explain its interaction with cancer cells to hinder their existence. 6. How would increasing the concentration of these metals in the bacteria s environment affect their growth and development? 7. What metal was the most affective in destroying bacteria in your experiment? What elements of the periodic table are present in that metal. Research that metal and hypothesize what some characteristics of it are that may lead to its antibacterial properties. Extensions: - You know that certain metals can hinder the growth of bacteria, but can they kill bacteria. Try this experiment again, but this time introduce the metal after the bacteria has shown significant growth to see if the metal is able to kill of the already grown bacteria. - Coins have been made out of different combinations of different metals over the years. Test how coins of different ages would affect bacteria growth. - 7

Cell Culture. Suggested Age: years old. Time: 45 minutes preparation, 3-4 days for data collection. What is a Cell Culture?

Cell Culture. Suggested Age: years old. Time: 45 minutes preparation, 3-4 days for data collection. What is a Cell Culture? Cell Culture Suggested Age: 10-14 years old Time: 45 minutes preparation, 3-4 days for data collection What is a Cell Culture? Cell Culture is a technique used to grow and identify microorganisms that

More information

BIOLOGY. Bacteria Growth Lab. Bacterial Growth. Slide 2 / 61. Slide 1 / 61. Slide 4 / 61. Slide 3 / 61. Slide 5 / 61. Slide 6 / 61

BIOLOGY. Bacteria Growth Lab. Bacterial Growth. Slide 2 / 61. Slide 1 / 61. Slide 4 / 61. Slide 3 / 61. Slide 5 / 61. Slide 6 / 61 Slide 1 / 61 Slide 2 / 61 New Jersey Center for Teaching and Learning Progressive Science Initiative This material is made freely available at www.njctl.org and is intended for the non-commercial use of

More information

WHY DO THEY PUT MINT IN TOOTHPASTE? WOULD GARLIC BE BETTER?

WHY DO THEY PUT MINT IN TOOTHPASTE? WOULD GARLIC BE BETTER? Activity 4.22 Student Sheet WHY DO THEY PUT MINT IN TOOTHPASTE? WOULD GARLIC BE BETTER? Purpose To investigate the antibacterial properties of plants. To develop practical skills. YOU NEED Agar plate seeded

More information

TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE

TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE GENERAL GUIDELINES: Safety Wear a lab coat and have your goggles on! ALWAYS disinfect the tables BEFORE and AFTER lab. Wash your hands with soap both BEFORE

More information

Culturing microorganisms may be hazardous

Culturing microorganisms may be hazardous Practical 8 - S(d) The Effect of Penicillin on Bacterial Growth In this practical focuses on the practical skills of: Planning defining the problem You will be developing other assessed skills throughout

More information

Microorganisms In Our Environment

Microorganisms In Our Environment PR015 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Microorganisms In Our Environment Teacher s Guidebook (Cat. # BE 106) think proteins!

More information

Heat Shock Proteins in Yeast (2012)

Heat Shock Proteins in Yeast (2012) MASSACHUSETTS INSTITUTE OF Technology Department of Biology Heat Shock Proteins in Yeast (2012) Summary Lydia Breen (Stoneham High School) Mary Brunson (Brookline High School) Yeast is a single-celled

More information

Aseptic Techniques. A. Objectives. B. Before coming to lab

Aseptic Techniques. A. Objectives. B. Before coming to lab Aseptic Techniques A. Objectives Become familiar with 1. The ubiquity of microorganisms (see Note 1) 2. Aseptic techniques (see Note 2) 3. Standard methods for growing/observing microorganisms (see Note

More information

Microbiological Methods

Microbiological Methods Microbiological Methods Making Media Pouring Culture Plates Sterile Technique Inoculating Plates and Culture Tubes Use of a Plate Counter to Estimate Microbial Population Densities Culturing Microorganisms

More information

ECOS Inquiry Template

ECOS Inquiry Template ECOS Inquiry Template 1. Contributor s Name: NATHAN GORDON 2. Name of Inquiry: NOT TOO HOT, NOT TOO COLD: THE EFFECTS OF TEMPERATURE ON SOIL BACTERIA 3. Goals and Objectives: a. Inquiry Questions: 1. How

More information

Bacterial Transformation: Unlocking the Mysteries of Genetic Material

Bacterial Transformation: Unlocking the Mysteries of Genetic Material PR009 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Bacterial Transformation: Unlocking the Mysteries of Genetic Material Teacher s Guidebook

More information

Bacterial Plate Preparation. ~ Using aseptic techniques ~

Bacterial Plate Preparation. ~ Using aseptic techniques ~ Bacterial Plate Preparation ~ Using aseptic techniques ~ Bacterial Plates Laboratory and research scientists have to prepare nutrient media to grow specific strains of bacteria for their research. To do

More information

Microbiological Methods

Microbiological Methods Microbiological Methods Making Media Pouring Culture Plates Sterile Technique Inoculating Plates and Culture Tubes Use of a Plate Counter to Estimate Microbial Population Densities Sterile Technique Sterile

More information

LABORATORY 5: TRANSFORMING BACTERIA WITH THE LIGATION PRODUCTS

LABORATORY 5: TRANSFORMING BACTERIA WITH THE LIGATION PRODUCTS LABORATORY 5: TRANSFORMING BACTERIA WITH THE LIGATION PRODUCTS So far in your quest to clone a gene you have produced recombinant plasmids and verified that you made the para-r plasmid containing the rfp

More information

DNA TRANSFORMATION OF BACTERIA RED COLONY REVISED 3/2003

DNA TRANSFORMATION OF BACTERIA RED COLONY REVISED 3/2003 DNA TRANSFORMATION OF BACTERIA RED COLONY REVISED 3/2003 Prepared by the Office of Biotechnology, Iowa State University TEACHER PREPARATION AND INSTRUCTION GUIDE Preparation for the DNA transformation

More information

Isolation & Characterization of Bacteria

Isolation & Characterization of Bacteria PR025 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Isolation & Characterization of Bacteria Teacher s Handbook (Cat. # BE 204) think proteins!

More information

BACTERIAL TRANSFORMATION LESSON PLAN

BACTERIAL TRANSFORMATION LESSON PLAN BACTERIAL TRANSFORMATION LESSON PLAN Primary Learning Outcomes: Understanding the process of bacterial genetic engineering through plasmid insertion. High School Georgia Performance Standards SCSh2. Students

More information

PURE CULTURE TECHNIQUES

PURE CULTURE TECHNIQUES PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,

More information

ASEPTIC TRANSFER & PURE CULTURE TECHNIQUES

ASEPTIC TRANSFER & PURE CULTURE TECHNIQUES ASEPTIC TRANSFER & PURE CULTURE TECHNIQUES GENERAL GUIDELINES & REMINDERS: SAFETY: NO EATING OR DRINKING IN THE LAB! Wash your hands with soap both BEFORE and AFTER lab, and, in addition, when you have

More information

LABORATORY #2 -- BIOL 111 BACTERIAL CULTIVATION & NORMAL FLORA

LABORATORY #2 -- BIOL 111 BACTERIAL CULTIVATION & NORMAL FLORA LABORATORY #2 -- BIOL 111 BACTERIAL CULTIVATION & NORMAL FLORA OBJECTIVES After completing this exercise you should be able to: 1. Identify various types of media 2. Isolate bacteria using aseptic technique.

More information

Bacterial Transformation Lab - pglo

Bacterial Transformation Lab - pglo Bacterial Transformation Lab - pglo Name: Date: Pre-Lab Score: Lab Overview: In this investigation, you will gain an understanding of the techniques of culturing E. coli bacteria and transforming using

More information

Pacing/Teacher's Notes

Pacing/Teacher's Notes Slide 1 / 31 New Jersey Center for Teaching and Learning Progressive Science Initiative This material is made freely available at www.njctl.org and is intended for the non-commercial use of students and

More information

Biotechnology In Your Mouth

Biotechnology In Your Mouth PR005 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Biotechnology In Your Mouth Teacher s Guidebook (Cat. # BE 102) think proteins! think

More information

CULTURING MICROORGANISMS

CULTURING MICROORGANISMS CULTURING MICROORGANISMS Question practice Name: Class: Date: Time: 24 minutes Marks: 24 marks Comments: BIOLOGY ONLY Page of 2 The diagram shows a method used to grow pure cultures of a bacterium. (a)

More information

Biology Class The Evolution Unit Lessons 7-12

Biology Class The Evolution Unit Lessons 7-12 Name: Hour: Teacher: ROZEMA Biology Class The Evolution Unit Lessons 7-12 Last Round We Figured Out: How common is Addie s problem? Could it happen to me? Where are bacteria? How do antibiotics work? How

More information

MICROBIOLOGY #2 PREPERATION AND STERILIZATION OF CULTURE MEDIA

MICROBIOLOGY #2 PREPERATION AND STERILIZATION OF CULTURE MEDIA MICROBIOLOGY #2 PREPERATION AND STERILIZATION OF CULTURE MEDIA When we receive a sample (ex. Urine sample) for detection, we cannot gram stain it right away if it requires to be inoculated because when

More information

Bacteria and other microbes have particular requirements for growth When they reside in and on our bodies or in the environment, they harvest their

Bacteria and other microbes have particular requirements for growth When they reside in and on our bodies or in the environment, they harvest their Bacteria and other microbes have particular requirements for growth When they reside in and on our bodies or in the environment, they harvest their food from us or from the environment When we grow bacteria

More information

Sixth Grade Science BLOCK 2 ASSESSMENT Simple Organisms

Sixth Grade Science BLOCK 2 ASSESSMENT Simple Organisms Sixth Grade Science BLOCK 2 ASSESSMENT Simple Organisms 2008-09 Directions: Choose the best answer for each question. Then mark your choice on your answer sheet. 1. Which of the following best describes

More information

2 Creating Genetically Modified Bacteria Gl o w - i n-th e - d a r k rabbits, pigs, and mice may sound like something out

2 Creating Genetically Modified Bacteria Gl o w - i n-th e - d a r k rabbits, pigs, and mice may sound like something out 2 Creating Genetically Modified Bacteria Gl o w - i n-th e - d a r k rabbits, pigs, and mice may sound like something out of a science fiction movie, but because of genetic modification, these animals

More information

MOLEBIO LAB #12: Bacterial Culture Techniques Part I

MOLEBIO LAB #12: Bacterial Culture Techniques Part I MOLEBIO LAB #12: Bacterial Culture Techniques Part I Introduction: This lab introduces an introduction to plating and culturing E. coli on LB agar plates such that single cells can be isolated from one

More information

KEY. Biology Baseline Cornerstone Assessment: Part A. Experimental Design

KEY. Biology Baseline Cornerstone Assessment: Part A. Experimental Design Biology Baseline Cornerstone Assessment: Part A. Experimental Design Directions: Read the paragraph below and then respond to the questions. Students in a biology class were discussing outbreaks of food-borne

More information

90927 Demonstrate understanding of biological ideas relating to micro-organisms COLLATED QUESTIONS

90927 Demonstrate understanding of biological ideas relating to micro-organisms COLLATED QUESTIONS 90927 Demonstrate understanding of biological ideas relating to micro-organisms COLLATED QUESTIONS DIGESTION AND REPRODUCTION PROCESSES (2013:1) (a) Describe the processes of digestion and reproduction

More information

The ramylase Project by Ellyn Daugherty

The ramylase Project by Ellyn Daugherty PR078 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name The ramylase Project by Ellyn Daugherty Transformation of E. coli with pamylase (Lab

More information

Cloning a Fluorescent Gene

Cloning a Fluorescent Gene Cloning a Fluorescent Gene Laboratory Protocols Handout v1.10 Table of Contents Lab 1: Pipettes and Pipetting... 2 Lab 2: Polymerase Chain Reaction... 5 Lab 3: Ligation... 7 Lab 4: Transformation... 9

More information

Principle of Lab. Safety

Principle of Lab. Safety Sulaimani University College of Pharmacy Principle of Lab. Safety Dr. Abdullah Ahmed Hama 1 1. Wear appropriate clothing and shoes to the laboratory. Shoes must completely cover the feet to provide protection

More information

Task 1: Crowded plate technique

Task 1: Crowded plate technique The Accidental Discovery For students Task 1: Crowded plate technique Do you recollect from previous year s knowledge, that microorganisms are present everywhere in nature? A variety of microorganisms

More information

Making Saline SOLUTION. Lab Number 2 Part 1

Making Saline SOLUTION. Lab Number 2 Part 1 Making Saline SOLUTION Lab Number 2 Part 1 Purpose The purpose of part 1 of this lab is to learn the proper way to make reagents that are needed for labs. Materials Need for the Lab are: Volumetric flasks

More information

UNIVERSITEIT GENT. Laboratory of Microbiology K.L. Ledeganckstr. 35 B-9000 Gent (BELGIUM) SOP. Standard Operating Procedure.

UNIVERSITEIT GENT. Laboratory of Microbiology K.L. Ledeganckstr. 35 B-9000 Gent (BELGIUM) SOP. Standard Operating Procedure. SOP Standard Operating Procedure Author: Acronym: Date last modified: Geert Huys ASIARESIST-PRES 20-11-2002 Title: PRESERVATION OF BACTERIA USING COMMERCIAL CRYOPRESERVATION SYSTEMS References: Reviewed

More information

Lab 5/5a Transformation of E. coli with a Recombinant Plasmid

Lab 5/5a Transformation of E. coli with a Recombinant Plasmid Lab 5/5a Transformation of E. coli with a Recombinant Plasmid Lab 2 Pre Lab Readiness Familiarity and Proper use of micropipettes Remember the 1 st and 2 nd stops Aseptic Technique Antibiotic Resistance

More information

PreLab Activity: Read through the entire lab! Come prepared to explain to your group what this lab is about

PreLab Activity: Read through the entire lab! Come prepared to explain to your group what this lab is about BIO101Lab weeks 4 and 5 PreLab Activity: Read through the entire lab! Come prepared to explain to your group what this lab is about The Evolution of the bacterium, Pseudomonas fluorescens Introduction

More information

MATERIALS (PER GROUP):

MATERIALS (PER GROUP): Mold Control INTRODUCTION In this activity, your class will test four different cleaners and then will determine which cleaner is most effective for controlling mold. You will analyze a series of dilutions

More information

Determination of MIC & MBC

Determination of MIC & MBC 1 Determination of MIC & MBC Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight

More information

Determination of MIC & MBC

Determination of MIC & MBC 1 Determination of MIC & MBC Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight

More information

Level 1 Biology, 2012

Level 1 Biology, 2012 90927 909270 1SUPERVISOR S Level 1 Biology, 2012 90927 Demonstrate understanding of biological ideas relating to micro-organisms 9.30 am Thursday 15 November 2012 Credits: Four Achievement Achievement

More information

Fig. 4.1 shows the process used to produce penicillin. waste gases

Fig. 4.1 shows the process used to produce penicillin. waste gases 1 Penicillin is an antibiotic produced by the fungus Penicillium chrysogenum. Fig. 4.1 shows the process used to produce penicillin. cooling water out waste gases water jacket fungus and nutrients temperature

More information

Reading the River, Summer A Study of Duck Creek Grades 7-8. Jim Young St. Joseph School Campbell County, Kentucky

Reading the River, Summer A Study of Duck Creek Grades 7-8. Jim Young St. Joseph School Campbell County, Kentucky Reading the River, Summer 2002 A Study of Duck Creek Grades 7-8 Jim Young St. Joseph School Campbell County, Kentucky Study of Duck Creek The eighth and seventh grade students at Saint Joseph School, Cold

More information

DESIGNING A CONTROLLED EXPERIMENT

DESIGNING A CONTROLLED EXPERIMENT DESIGNING A CONTROLLED EXPERIMENT STEP 1: DEFINING THE PROBLEM Scientific Method Every scientific investigation begins with a question that the scientist wants to answer. The questions addressed by scientific

More information

Section A: Prokaryotes Types and Structure 1. What is microbiology?

Section A: Prokaryotes Types and Structure 1. What is microbiology? Section A: Prokaryotes Types and Structure 1. What is microbiology? 2. Compare and contrast characteristics of each bacterial type: Eubacteria and Archaebacteria. Eubacteria Both Archaebacteria 3. Label

More information

Bacterial Transformation with pglo, Part 1

Bacterial Transformation with pglo, Part 1 Biology 211 Bacterial Transformation with pglo, Part 1 OBJECTIVES: Practice formulating predictions Describe the principles of bacterial transformation. Explain the procedure for gene transfer using plasmid

More information

Aquatic Ecosystem for Middle School

Aquatic Ecosystem for Middle School Aquatic Ecosystem for Middle School Teacher Edition By: Melissa Wirth and Susan Cline *This lesson assumes that students understand dissolved oxygen is a requirement of aquatic life. NGSS Alignment: MS-LS2-4

More information

pglo Transformation 1. Do the genetic transformation. 2. Determine the degree of success in your efforts to genetically alter an organism.

pglo Transformation 1. Do the genetic transformation. 2. Determine the degree of success in your efforts to genetically alter an organism. Introduction to Transformation pg Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides the instructions for making

More information

CHEMICAL ENGINEERING LABORATORY CHEG 4137W. Bioreactor

CHEMICAL ENGINEERING LABORATORY CHEG 4137W. Bioreactor CHEMICAL ENGINEERING LABORATORY CHEG 4137W Bioreactor Objective: The laboratory has acquired a bioreactor for the purpose of growing cell cultures. In the initial attempt to grow E. coli, an M9 growth

More information

number Done by Corrected by Doctor

number Done by Corrected by Doctor L number Lab 2 Done by حسام أبو عوض Corrected by Mahdi sharawi Doctor In many cases we need to identify the type of bacteria causing an infection in order to be able to choose the right medication (antibiotic).

More information

How to perform a Gram Stain. Jasleen Singh

How to perform a Gram Stain. Jasleen Singh How to perform a Gram Stain Jasleen Singh Table of Contents iii Table of Contents Table of Contents... iii Introduction... 5 Terminology... 7 Terms to be familiar with... 7 Gram Staining... 8 What is

More information

Heterotrophic Bacteria

Heterotrophic Bacteria , m-hpc, 8242 DOC316.53.01225 Pour Plate Method Method 8242 m-hpc Scope and Application: For water and wastewater. Test preparation Introduction Before starting the test: The Pour Plate Method, also known

More information

LAB NOTES FOR EXAM 1 SECTION

LAB NOTES FOR EXAM 1 SECTION LAB NOTES FOR EXAM 1 SECTION EX. 2-1: DIVERSITY AND UBIQUITY OF MICROOGANISMS Purpose: Microorganisms are found everywhere in the environment around us. To demonstrate this and to get a taste of the different

More information

Test Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002

Test Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002 Test Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002 FTTS-FA-002 Antibacterial Textiles for Medical Use Antibacterial Textiles suppress and even kill harmful bacteria

More information

Level 1 Biology, 2015

Level 1 Biology, 2015 90927 909270 1SUPERVISOR S Level 1 Biology, 2015 90927 Demonstrate understanding of biological ideas relating to micro-organisms 2.00 p.m. Friday 20 November 2015 Credits: Four Achievement Achievement

More information

The Grand Prismatic Spring in Yellowstone National Park is the largest hot spring in the United States The vivid colors in the spring are the result

The Grand Prismatic Spring in Yellowstone National Park is the largest hot spring in the United States The vivid colors in the spring are the result The Grand Prismatic Spring in Yellowstone National Park is the largest hot spring in the United States The vivid colors in the spring are the result of pigmented bacteria in the microbial mats that grow

More information

pglo Transformation Lab Integrated Science 4 Redwood High School Name Per:

pglo Transformation Lab Integrated Science 4 Redwood High School Name Per: pglo Transformation Lab Integrated Science 4 Redwood High School Name Per: n Introduction To Transformation In this lab you will perform a procedure known as a genetic transformation. Remember that a gene

More information

STUDENT PAGE. Using Sterile Technique to Inoculate Bacterial Plates

STUDENT PAGE. Using Sterile Technique to Inoculate Bacterial Plates Using Sterile Technique to Inoculate Bacterial Plates A sterile, or aseptic, technique is used to prevent microbial organisms from contaminating any surface other than the specific location where they

More information

2. Be sure to wear gloves and safety goggles, especially when using the disinfectant.

2. Be sure to wear gloves and safety goggles, especially when using the disinfectant. Mold Control INTRODUCTION In this activity, your class will test three different cleaners and then will determine which cleaner is most effective for controlling mold. You will analyze a series of dilutions

More information

MICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods

MICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods Issue No.29 / March 2010 MICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods By Dr Vincent TURBLIN, Deputy Regional Market Manager Poultry - CEVA Animal Health Asia Pacific Most

More information

foodproof StarPrep Two Kit Order No. S Quick Reference Procedure for Legionella

foodproof StarPrep Two Kit Order No. S Quick Reference Procedure for Legionella foodproof StarPrep Two Kit Order No. S 400 08 Quick Reference Procedure for Legionella Version 1, November 2017 A. Kit Contents / Storage and Stability Product Content Storage S 400 08 1 container with

More information

Contact Information: Laboratory Supervisor: Denise Kind Laboratory Manager: Mat Ashby

Contact Information: Laboratory Supervisor: Denise Kind Laboratory Manager: Mat Ashby Biology and Wildlife STANDARD OPERATING PROCEDURE Autoclaving Location(s): Murie 215 Chemical(s): None Specific Hazards: o steam improper use of autoclave can expose user to dangerous steam burns o extremely

More information

BASIC LABORATORY SKILLS. The Scientific Method. Describe the steps in the scientific method. Study Questions

BASIC LABORATORY SKILLS. The Scientific Method. Describe the steps in the scientific method. Study Questions UNIT III: Lesson 1: BASIC LABORATORY SKILLS The Scientific Method Competency/Objective: Describe the steps in the scientific method. Study Questions References 1. What are the steps of the scientific method?

More information

Lab Exercise #4 Microbial Control Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic

Lab Exercise #4 Microbial Control Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic I. OBJECTIVES: Investigate the effectiveness various agents of control. Assess the effectiveness of heat in killing vegetative

More information

Biology Lab Activity 4-5 DNA Transformation

Biology Lab Activity 4-5 DNA Transformation Biology Lab Activity 4-5 DNA Transformation Scientists can insert genes into bacteria. The genes inserted in the Indo-Blu process (this lab) are on a circular piece of DNA called a plasmid. (The plasmid

More information

Solar and SODIS: Creating Clean Water for the World

Solar and SODIS: Creating Clean Water for the World Solar and SODIS: Creating Clean Water for the World AUTHOR: Jamie Repasky DESCRIPTION: According to Nobel Laureate Richard Smalley, the number one and two challenges for humanity are energy and clean water.

More information

CHEMICAL ENGINEERING SENIOR LABORATORY CHEG Bioreactor

CHEMICAL ENGINEERING SENIOR LABORATORY CHEG Bioreactor 1 CHEMICAL ENGINEERING SENIOR LABORATORY CHEG 4139 Bioreactor Objective: The laboratory has acquired a bioreactor for the purpose of growing cell cultures. In the initial attempt to grow E. coli, an M9

More information

INTRODUCTION Sanitization sterilization Antibiotics Bactericidal Bacteriostatic Antiseptics disinfectants

INTRODUCTION Sanitization sterilization Antibiotics Bactericidal Bacteriostatic Antiseptics disinfectants INTRODUCTION Infectious agents on environmental surfaces, given the correct circumstances, may potentially find their way into an unsuspecting victim. Thus, it is important to keep the surfaces we regularly

More information

Transduction of an Antibiotic Resistance Gene. Background

Transduction of an Antibiotic Resistance Gene. Background I Student Guide 21-1128 Name------------ Date Transduction of an Antibiotic Resistance Gene Background Transduction is a natural method of gene transfer that occurs in bacteria. The key player in transduction

More information

RUNNING HEAD: Yeast: The Properties and Genetic Mutations Within

RUNNING HEAD: Yeast: The Properties and Genetic Mutations Within RUNNING HEAD: Yeast: The Properties and Genetic Mutations Within Yeast: The Properties and Genetic Mutations Within BIOL 263 004 Indiana University of Pennsylvania Meagan Lamar Yeast 2 Abstract The many

More information

What s the big deal with antibacterial soap? An inquiry lab on adaptation

What s the big deal with antibacterial soap? An inquiry lab on adaptation What s the big deal with antibacterial soap? An inquiry lab on adaptation Did you know? There is some evidence that triclosan, triclocarban and the other chemicals can disrupt hormone cycles and cause

More information

BIOLOGY 163 LABORATORY. THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014)

BIOLOGY 163 LABORATORY. THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014) BIOLOGY 163 LABORATORY THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014) Bacteria are single-celled prokaryotic organisms. As bacterial cells take in nutrients from their

More information

DESIGNING A CONTROLLED EXPERIMENT

DESIGNING A CONTROLLED EXPERIMENT AP BIOLOGY NAME DESIGNING A CONTROLLED EXPERIMENT STEP 1: DEFINING THE P ROBLEM Every scientific investigation begins with the question that the scientist wants to answer. The questions addressed by scientific

More information

M. Dalbey/Bio 105M Isolation of E. coli - Isolation of E. coli from an Environmental Sample

M. Dalbey/Bio 105M Isolation of E. coli - Isolation of E. coli from an Environmental Sample Isolation of E. coli from an Environmental Sample We want to expand our horizons a bit beyond the domesticated lab strains of E. coli. In this exercise you will isolate "wild" E. coli strains from an environmental

More information

Bt-Cry1F ELISA Kit Qualitative DAS ELISA for the detection of the Bt-Cry1F transgenic protein Catalog number: PSP 10301

Bt-Cry1F ELISA Kit Qualitative DAS ELISA for the detection of the Bt-Cry1F transgenic protein Catalog number: PSP 10301 List of contents Lot number Item 96 wells 288 wells 480 wells 4800 wells Antibody-coated 96-well microtiter plates 1 3 5 50 solid Peroxidase enzyme conjugate (1x, ready to use) 11 ml 33 ml 55 ml 550 ml

More information

Bacterial Transformation Using Fluorescent Protein Teacher Guide

Bacterial Transformation Using Fluorescent Protein Teacher Guide Bacterial Transformation Using Fluorescent Protein Teacher Guide sciencebridge PROTOCOL 2 Bacterial Transformation using Fluorescent Protein Central question How does a change in the genotype of an organism

More information

MiSP Evolution by Natural Selection / Bacterial Resistance Teacher Guide, L1 L3. Introduction

MiSP Evolution by Natural Selection / Bacterial Resistance Teacher Guide, L1 L3. Introduction MiSP Evolution by Natural Selection / Bacterial Resistance Teacher Guide, L1 L3 Introduction This unit uses the development of resistance to antimicrobials as an example of natural selection. The students

More information

Cell Growth and DNA Extraction- Technion igem HS

Cell Growth and DNA Extraction- Technion igem HS Growing Cells and DNA Extraction Goals 1. Become familiar with the process of growing bacteria 2. Get to know the DNA extraction process 3. Perform miniprep in the lab Keywords 1. Growth stages 6. Techniques

More information

Problems and profit with waste. Standard Grade Biology Biotechnology

Problems and profit with waste. Standard Grade Biology Biotechnology Problems and profit with waste Standard Grade Biology Biotechnology Learning Outcomes Describe some examples of the damage caused to the environment by disposal of untreated sewage. Give examples of diseases

More information

M I C R O B I O L O G I C A L T O O L S F O R Q U A L I T Y A S S U R A N C E I N H A T C H E R Y : Sampling Procedures

M I C R O B I O L O G I C A L T O O L S F O R Q U A L I T Y A S S U R A N C E I N H A T C H E R Y : Sampling Procedures Issue No.28 / January 2010 M I C R O B I O L O G I C A L T O O L S F O R Q U A L I T Y A S S U R A N C E I N H A T C H E R Y : Sampling Procedures by Dr Vincent TURBLIN Deputy Regional Market Manager Poultry

More information

NATURE OF MICROBES WORKBOOK

NATURE OF MICROBES WORKBOOK NATURE OF MICROBES WORKBOOK Name: Tutor Group: 1 Microbes and Mankind 4. NATURE OF MICROBES 1. OBJECTIVES: What are microbes and are there different types? How are they seen? How can they be grown? How

More information

Accugen Laboratories, Inc.

Accugen Laboratories, Inc. Lab# 97478-2 www.accugenlabs.com Page 1 of 7 Accugen Laboratories, Inc. FINAL REPORT ASTM G22 ASTM Designation: G22-76(1996) "Standard Practice for determining Resistance of Plastics to Bacteria (Withdrawn

More information

Project 7: Wound Cultures and Identification

Project 7: Wound Cultures and Identification Project 7: Wound Cultures and Identification Readings: https://labtestsonline.org/understanding/analytes/wound-culture/tab/test Identification of Gram-Positive & Gram-Negative Bacteria Guide to laboratory

More information

Kit Information 4 Introduction. 4 Kit Contents, Storage, and Testing Conditions. 4 Principle 4 Applicability. 5 Precautions Sample Preparation 6

Kit Information 4 Introduction. 4 Kit Contents, Storage, and Testing Conditions. 4 Principle 4 Applicability. 5 Precautions Sample Preparation 6 Contents 3. Kit Information 4 Introduction. 4 Kit Contents, Storage, and Testing Conditions. 4 Principle 4 Applicability. 5 Precautions.......................... 5 Sample Preparation 6 Peel Plate EB Test

More information

Body Buddies: The Microbes that Live In and On Us

Body Buddies: The Microbes that Live In and On Us Body Buddies: The Microbes that Live In and On Us This article is provided courtesy of the American Museum of Natural History. Scientists use microscopes to examine things that are too small to be seen

More information

Bt-Cry1F ELISA Kit Quantitative DAS ELISA for the detection of the Bt-Cry1F transgenic protein Catalog number: PSP 11700

Bt-Cry1F ELISA Kit Quantitative DAS ELISA for the detection of the Bt-Cry1F transgenic protein Catalog number: PSP 11700 List of contents Lot number Item 96 wells 288 wells 480 wells Antibody-coated 96-well microtiter plates 1 3 5 Peroxidase enzyme conjugate (1x, ready to use) 11 ml 33 ml 55 ml TMB substrate solution 25

More information

COUNT METHOD 5.0 OBJECTIVES 5.1 INTRODUCTION 5.2 PRINCIPLE. Structure

COUNT METHOD 5.0 OBJECTIVES 5.1 INTRODUCTION 5.2 PRINCIPLE. Structure Food Microbiology EXPERIMENT 5 STANDARD PLATE COUNT METHOD Structure 5.0 Objectives 5.1 Introduction 5.2 Principle 5.3 Materials Required 5.4 Procedure 5.4.1 E-coli Culture 5.4.2 Food Samples 5.5 Observations

More information

EZ BioResearch Bacteria Science Kit (10-Pack) (Pre-poured LB Agar Plates and Cotton Swabs) EZ Science Fair Project E-Book 2015 V-3

EZ BioResearch Bacteria Science Kit (10-Pack) (Pre-poured LB Agar Plates and Cotton Swabs) EZ Science Fair Project E-Book 2015 V-3 EZ BioResearch Bacteria Science Kit (10-Pack) (Pre-poured LB Agar Plates and Cotton Swabs) EZ Science Fair Project E-Book 2015 V-3 www.ezbioresearch.com Tel: 1(800) 637-0262 Fax: 1(877) 693-4868 About

More information

Prepared by the Office of Biotechnology, Iowa State University DRAFT 4/03

Prepared by the Office of Biotechnology, Iowa State University DRAFT 4/03 RECOMBINANT DNA: DUAL ANTIBIOTIC-RESISTANCE GENES Prepared by the Office of Biotechnology, Iowa State University DRAFT 4/03 ** Portions of this protocol were adapted from DNA Science: A First Course in

More information

Lab Three :. Sensitivity test:

Lab Three :. Sensitivity test: Lab Three :. Sensitivity test: Or Diffusion Test: Antibiotic sensitivity test: is a laboratory method for determining the susceptibility of organisms to therapy with antibiotics, Antibiotic susceptibility

More information

OPERATOR S MANUAL FOR DETECTION AND ENUMERATION OF ENTEROBACTERIACEAE BACTERIA IN FOOD, SERIAL DILUTIONS OF FOOD, AND ENVIRONMENTAL SPONGE SAMPLES

OPERATOR S MANUAL FOR DETECTION AND ENUMERATION OF ENTEROBACTERIACEAE BACTERIA IN FOOD, SERIAL DILUTIONS OF FOOD, AND ENVIRONMENTAL SPONGE SAMPLES OPERATOR S MANUAL FOR DETECTION AND ENUMERATION OF ENTEROBACTERIACEAE BACTERIA IN FOOD, SERIAL DILUTIONS OF FOOD, AND ENVIRONMENTAL SPONGE SAMPLES Contents 3. Kit Information 4 Introduction. 4 Kit Contents,

More information

Inquiry CFA #1. Student Name: Date: Teacher Name: Jasmine Straughter. Score:

Inquiry CFA #1. Student Name: Date: Teacher Name: Jasmine Straughter. Score: Inquiry CFA #1 Student Name: Teacher Name: Jasmine Straughter Date: Score: 1 ) If the bird population is directly proportional to the average monthly temperature, which month would have the LOWEST temperature?

More information

Laboratory Biosafety Plan

Laboratory Biosafety Plan DUKE NEUROBIOLOGY SCHOOL OF MEDICINE Laboratory Biosafety Plan Laboratory of Boris Kantor Laboratory Biohazards: Biosafety Level 1 Recombinant DNA cloning in E. coli K-12 and derivatives Adeno-Associated

More information

CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS

CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly

More information

Design and Equipment

Design and Equipment Cell Culture Design and Equipment Laboratory Design Microbiological Safety Cabinets Centrifuges Incubators Work Surfaces and Flooring Plasticware and Consumables Care and Maintenance of Laboratory Areas

More information

Mission X: Train Like an Astronaut What's in your Petri

Mission X: Train Like an Astronaut What's in your Petri Mission X: Train Like an Astronaut What's in your Petri BUGS IN SPACE PART 2 EDUCATOR SECTION (PAGES 1-12) STUDENT SECTION (PAGES 13-21) Background Microbes live everywhere! While many microbes on Earth

More information

Microbial Growth and Assessing Mutagenic Properties in Plastic Bottles

Microbial Growth and Assessing Mutagenic Properties in Plastic Bottles Microbial Growth and Assessing Mutagenic Properties in Plastic Bottles By: Eliza Chevalier, Eliza Doncaster, and Steven Lamonde 23 September 2013 Introduction It is often debated whether plastic has adverse

More information