Thermophilic microorganisms capable of degrading biopolymers
|
|
- Jean Grant
- 6 years ago
- Views:
Transcription
1 Thermophilic microorganisms capable of degrading biopolymers Ilya V. Kublanov Laboratory of hyperthermophilic microbial communities, Winogradsky Institute of Microbiology, Russian Academy of Sciences
2 Summary Thermophilic microorganisms Sources of isolation of thermophilic microorganisms Utilization of thermophilic microorgansisms and their enzymes: advantages Thermophilic microorganisms capable of biopolymers degradation Hydrolases-coding genes in genomes of thermophilic microorganisms capable of biopolymers degradation 2
3 Thermophilic microorganisms hyperthermophiles extreme thermophiles Pyrolobus fumarii is a champion (at least, at present time), H 2 /O 2, NO 2-3, S 2 O C (opt = 106 C). Stetter et al, Extremophiles, psychrophiles Methanopyrus kandleri strain 116 is growing up to 122 C (opt = 105 C) at 40 MPa. Kurr et al, Arch. Microbiol.,1991. Takai et al, PNAS, C 80 C 70 C moderate thermophiles mesophiles Optimal temperature 40 C 15 C Bacteria are able to grow at any temperature at which there is liquid water Brock, BUT: Above 110 C aminoacids and other metabolites become highly unstable => temperature limit is not far away from 113 C Jaenicke,
4 Thermophilic microorganisms 16S rrna gene-based tree of life Woese et al., 1990, edited by Jurgens,
5 Sources of isolation of thermophilic microorganisms Hightemperature subsurface biosphere Shallow-water submarine hot vents Deep-sea hot vents Terrestrial hot springs 5
6 Sources of isolation of thermophilic microorganisms Deep-sea Shallow Terrestrial Subsurface 6
7 Advantages of thermophiles utilization Contamination risk is low High temperature allows to work with higher substrates concentrations due to viscosity decrease Industrial processes sometimes require high temperatures Less studied => many new, undiscovered enzymes Produces thermostable enzymes - thermozymes 7
8 Advantages of thermozymes utilization Thermostability Higher tolerance to denaturing agents (detergents, solvents) High temperature allows to work with higher substrates concentrations due to viscosity decrease Purification of recombinant enzymes is simplified (thermal treatment) 8
9 Thermophilic microorganisms in INMI RAS Thermophilic and hyperthermophilic bacteria and archaea (> 350 strains) Many represent new taxonomic groups (species, genera, families, orders, classes), one novel phylum Represent new metabolic groups Source of novel thermostable enzymes 9
10 Thermophilic microorganisms capable of bipolymers degradation Polymeric substrates used by thermophiles from our culture collection: Carbohydrates Cellulose and derivatives Xylan Agarose Chitin Lichenan Laminarin Proteins In situ enrichments cultivation of tubes with insoluble organic substrates in the hot springs Immediately after cultivation: detection of hydrolitic activity detection of dominating microorganisms isolation of dominating microorganisms capable of growing on biopolimers in pure cultures Kublanov et al., AEM, 2009, 75, Lipids 10
11 Thermophilic microorganisms capable of bipolymers degradation Cellulose degradation (incubation for 1.5 days at 70 о С) Beta-keratin (feathers) degradation (incubation for 3 days at 65 о С) A - control Б - Caldicellulosiruptor kronotskyensis (Miroshnichenko et al, 2008, 58: В Dictyoglomus sp. (unpublished) Caldanaerobacter sp. Strain (unpublished) feathers 11
12 Thermophilic microorganisms capable of bipolymers degradation Several genomes of our thermophilic and hyperthermophilic hydrolytic microorganisms were sequences and annotated Organism Domen Novel Topt C Were sequenced status Desulfurococcus kamchatkensis Thermococcus sibiricus Acidilobus aceticus Desulfurococcus fermentans Melioribacter roseus Archaea species Bioengineering Center, RAS Archaea species 78 Bioengineering Center, RAS Archaea order 82.5 Bioengineering Center, RAS Archaea species 80 Virginia Bioinformatics Institute + JGI Bacteria phylum Bioengineering Center, RAS Published in 2009 Published in 2009 Published in 2010 Under analysis Under analysis 12
13 Thermophilic microorganisms capable of bipolymers degradation Desulfurococcus kamchatkensis First archaeum, growing on keratin! Substrates: Alpha-keratin Gelatin Kublanov et al., IJSEM, 2009, 59, Hyperthermophile (T, o C ) Neutrophile (ph opt 6.5) Obligate anaerobe Casein Albumin Dextran Sucrose 13
14 Hydrolases-coding genes in genomes of thermophilic microorganisms capable of biopolymers degradation Genome of Desulfurococcus kamchatkensis In collaboration with CB Contains a number of peptidases-coding genes, including those hydrolyzing alphakeratin Contains genes of alpha-ghs which are in accordance with substrate specificity of D. kamchatkensis Ravin et al., J. Bacteriol : Most interesting hydrolases Dkam_0406 Glycoside Hydrolase 53, endo-β-1,4- First GH53, found in extracellular galactanase, EC Archaea Dkam_0582 Alpha-glucosidase, EC or EC The novel GH family introcellular Dkam_0433 Trypsin-like serine protease S1 Trypsin-like intracellular Dkam_1142 Cystein aminopeptidase, C15 The nearest - bacterial ND Dkam_1274 Subtilisin-like serine endopeptidase, S8A Very distant to other extracellular 14
15 Thermophilic microorganisms capable of bipolymers degradation Thermococcus sibiricus Isolated from high-temperature oil reservoir in Western Siberia Russia, from the depth 2350 m (T=85 o C) Substrates: Amorphous cellulose Miroshnichenko et al. Extremophiles, 2001, 5, Hyperthermophile (T, o C ) Neutrophile (ph opt 7.3) Obligate anaerobe Agarose Dextran Olive oil 15
16 Hydrolases-coding genes in genomes of thermophilic microorganisms capable of biopolymers degradation Genome of Thermococcus sibiricus In collaboration with CB Contains 15 genes encoding esterases (and lipases in particular), among them 4 extracellular. No beta-oxidation enzymes. T. sibiricus was found to be able to grow on olive oil and glycerol, but not on fatty acids. Contains saccharolytic gene island - a region with many genes of extracellular and intracellular GHs and transporters. Acquired by lateral gene transfer, presumably, from extremally thermophilic bacteria of phylum Thermotogae. Tsib_0325 Tsib_0326, Tsib_0327, Tsib_0328 Glycoside Hydrolase 50, betaagarase, EC Putative Glycoside Hydrolase 12, cellulase or novel GH Tsib_1454 Carboxylic ester hydrolase Very distant to other Mardanov et al., AEM, : Most interesting hydrolases The first GH50, found in Archaea. In general, the family consist of 58 proteins, non of them was characterized Extremely distant form other GH12. CAZy does not put them into GH12 16
17 Thermophilic microorganisms capable of bipolymers degradation New order Acidilobales Acidilobus aceticus Prokofeva et al. IJSEM, 2000, 50, Acidilobus saccharovorans Prokofeva et al. IJSEM, 2009, 59, Hyperthermophiles (T, o C ) Acidophiles (ph ) Obligate anaerobes Substrates: Lichenan Laminarin Sucrose Lactose Arbutin Xylan Starch Beef extract 17
18 Hydrolases-coding genes in genomes of thermophilic microorganisms capable of biopolymers degradation Genome of Acidilobus saccharovorans In collaboration with CB Contains endopeptidase-coding genes, including rear acid endopeptidase thermopsin. Contains many GH-coding genes. Due to distant phylogenetic position and acidophilic origin of A. saccharovorans its GHs might possess novel features, like acid tolerance. Asac_0635 Serine protease Very distant to other Asac_0641 Thermopsin-like protease, A5 Very distant to other extracellular Asac_0652 pepstatin-insensitive carboxyl formerly known as A4, extracellular proteinase, G1, well-known in fungi Asac_0737 Serine protease, putative S53 formerly known as A4 probably extracellular Asac_1420 Serine protease, putative S53 formerly known as A4 extracellular Asac_0354 Glycoside Hydrolase 15, putative glucoamylase, EC Mardanov et al., AEM, : Most interesting hydrolases Very distant to other extracellular 18
19 Hydrolases-coding genes in genomes of thermophilic microorganisms capable of biopolymers degradation Genome of Acidilobus saccharovorans Asac_0772 Glycoside Hydrolase 31, putative α-glucosidase, EC Very distant to other introcellular Asac_0825 Glycoside Hydrolase 57, putative alpha-amylase Very distant to other extracellular Asac_1074 Putative Glycoside Hydrolase 13, α-amylase, EC Very distant to other introcellular Asac_1367 Glycoside Hydrolase 38, α-mannosidase, EC Very distant to other introcellular Asac_1378 Glycoside Hydrolase 3, putative β-xylosidase Very distant to other introcellular Asac_1380 Glycoside Hydrolase 12, putative cellulase, EC Very distant to other extracellular Asac_1386 Glycoside Hydrolase 12, putative cellulase, EC Very distant to other introcellular Asac_1415 Glycoside Hydrolase 12, putative cellulase, EC Very distant to other introcellular Mardanov et al., AEM, : Most interesting hydrolases 19
20 Thermophilic microorganisms capable of bipolymers degradation Desulfurococcus fermentans Substrates: Cellulose Agarose Arbutin Sucrose Starch Perevalova et al., IJSEM, 2005, 55, Hyperthermophile (T, o C ) Neutrophile (ph opt 6.0) Obligate anaerobe Alpha-keratin Casein hydrolysate 20
21 Hydrolases-coding genes in genomes of thermophilic microorganisms capable of biopolymers degradation Desulfurococcus fermentans starch First archaeum growing on cellulose! cellulose control Genome of Desulfurococcus fermentans In collaboration with Contains several GH-coding genes. Despite the ability to grow on cellulose, no cellulases were found so far great challenge for us 21
22 Thermophilic microorganisms in INMI RAS Melioribacter roseus represents a novel phylum Novel phylum 16S rrna gene-based tree of life 22
23 Thermophilic microorganisms capable of bipolymers degradation Melioribacter roseus New Phylum! Podosokorskaya, Kublanov et al., MS in preparation Moderately thermophile (T, o C ) Neutrophile (ph opt 7.5) Facultative anaerobe Dissimilatory Fe(III) reduction Substrates: Cellulose Starch Xylan Dextran Glycogen Lichenan Gelatin 23
24 Hydrolases-coding genes in genomes of thermophilic microorganisms capable of biopolymers degradation Genome of Melioribacter roseus In collaboration with CB Contains about 90 GH-coding genes, that`s comparable to the most active polysaccharide-degrading microorganisms. Also, possess many GTs (glycosyl transferases), CE (carbohydrate esterases) and PL (polysaccharide lyases) Mros Chyd Cthe Rmar Dtur Ctha According to CAZy database. # of putative GH genes # of GHs with uncertain GH fam. # of GHs, placed into families 24
25 Hydrolases-coding genes in genomes of thermophilic microorganisms capable of biopolymers degradation Genome of Melioribacter roseus Most interesting hydrolases Mros505 Glycoside Hydrolase 5, putative cellulase, EC extracellular Mros508 Glycoside Hydrolase 43/32/68 A novel family, probably intracellular Mros512 Glycoside Hydrolase 5, mannanase endo- or exo- Very distant to other extracellular Mros548 Glycoside Hydrolase 43/32/68 A novel family, probably intracellular Mros754 Glycoside Hydrolase 5, putative cellulase, EC extracellular Mros758 Glycoside Hydrolase 9, putative cellulase, EC Very distant to other extracellular Mros971 Glycoside Hydrolase 42, b-agarase, EC Very distant to other extracellular Mros982 Glycoside Hydrolase 30, 2 domains A novel family, probably extracellular Mros969 Glycoside Hydrolase 43 Mros1588 Glycoside Hydrolase 47, a-mannosidase, EC Very distant to other extracellular Mros1596 Glycoside Hydrolase 92, putative mannosidase Very distant to other extracellular Mros2628 Glycoside Hydrolase 5 Very distant to other intracellular 25
26 Cellulases of Melioribacter roseus 45 kda Zymogram. Substrate CMC. Incubation: 77 C, ph 7.7, 3 h. 1. Markers 2. aerobic cells 3. anaerobic cells 4. aerobic cells. Preincubation at 96 C, 5` 5. aerobic cells. Preincubation at 96 C, 10` 6. aerobic cells. Preincubation at 96 C, 30` M. Roseus is growing optimally at 55 C Its cellulases (presumably GH5) are stable at 96 C 26
27 Novel hydrolases, found in genomes of our microorganisms Enzyme class GH5, GH9, GH12 - endoglucanase (cellulase), exoglucanase, b-mannosidase, licheninase, endoxylanase and other activities GH1 - b-galactosidase, b-glucosidase and other activities GH42, GH50 - b-agarase GH13, GH57 - a-amylase, pullulanase and other activities GH38, GH47, GH92 - different mannosidases A5 thermopsin, an acid endopeptidase S8A - subtilisin-like serine endopeptidase Found in: Thermococcus sibiricus, Acidilobus saccharovorans, Melioribacter roseus, Caldicellulosiruptor kronotskiensis Thermococcus sibiricus, Acidilobus saccharovorans, Desulfurococcus kamchatkensis, Desulfurococcus fermentans, Melioribacter roseus Thermococcus sibiricus, Melioribacter roseus Thermococcus sibiricus, Acidilobus saccharovorans, Desulfurococcus kamchatkensis, Desulfurococcus fermentans, Melioribacter roseus Melioribacter roseus, Acidilobus saccharovorans, Acidilobus saccharovorans Desulfurococcus kamchatkensis and other And many others! 27
28 Possible applications: Wastes utilization Biomass conversion Biofuels Food bioprocessing Detergents 28
29 Thank you! Laboratory of hyperthermophilic microbial communities, INMI RAS Laboratory: E.A. Bonch-Osmolovskaya A.V. Lebedinskiy, T.G. Sokolova A.I. Slobodkin, N.A. Chernyh M.I. Prokofeva, G.B. Slobodkina I.V. Kublanov, O.A. Podosokorskaya S.N. Gavrilov, D. Kozhevnikova M.Yu. Merkel, A.A. Perevalova Funding: Russian Academy of Sciences Russian Foundation of Basic Research Russian Federation Ministry of Education and Science FP7-KBBE Hotzyme project systematic screening for novel hydrolases from hot environments (just started) 29
The HotZyme Newsletter
Issue 02 2012 The HotZyme Newsletter SYSTEMATIC SCREENING FOR NOVEL HYDROLASES FROM HOT ENVIRONMENTS Preface Dear readers, Welcome to the second newsletter of the HotZyme- project on Systematic screening
More informationDyadic Inside. The Power of Nature The Speed of Technology. Mark Emalfarb, Founder and CEO Dyadic International, Inc.
Dyadic Inside The Power of Nature The Speed of Technology Mark Emalfarb, Founder and CEO Dyadic International, Inc. 1 Safe Harbor Statement Certain statements contained in this presentation are forward-looking
More informationMolecular characterization and phylogenetic analysis of protease producing Streptomyces sp. isolated from mangrove sediments
Molecular characterization and phylogenetic analysis of protease producing Streptomyces sp. isolated from mangrove sediments M. Parthasarathy and J. Joel Gnanadoss* Department of Plant Biology and Biotechnology,
More informationThe HotZyme Newsletter
Issue 04 2014 The HotZyme Newsletter SYSTEMATIC SCREENING FOR NOVEL HYDROLASES FROM HOT ENVIRONMENTS From the Editor Dear readers, Welcome to the 12th edition of the HotZyme Newsletter. As we approach
More informationENVE 424 Anaerobic Treatment
ENVE 424 Anaerobic Treatment Lecture 3 The Microbiology of Anaerobic Treatment 2012 2013 Fall 27-28 Sept 2012 Assist. Prof. A. Evren Tugtas Anaerobic Digestion Ref: Gerardi M. H. The Microbiology of Anaerobic
More informationTransforming Industries With High Performance Enzymes. David P. Weiner, Ph.D. December 10, 2013
Transforming Industries With High Performance Enzymes David P. Weiner, Ph.D. December 10, 2013 Global Challenges & Opportunities 9000 8000 7000 Global Population (millions) 6000 5000 4000 3000 2000 1000
More informationENVIRONMENTAL PARAMETERS OF GROWTH
ENVIRONMENTAL PARAMETERS OF GROWTH The growth and survival of microorganisms are affected by the chemical and physical conditions of the external environment. Environmental factors which have significant
More informationTHERMOPHILIC ENZYMES FOR BIOMASS CONVERSION
Bioenergy- II: Fuels and Chemicals from Renewable Resources THERMOPHILIC ENZYMES FOR BIOMASS CONVERSION Dr. Francesco La Cara Institute of Protein Biochemistry C.N.R. Naples - Italy THERMOPHILIC ENZYMES
More informationENVIRONMENTAL PARAMETERS OF GROWTH
ENVIRONMENTAL PARAMETERS OF GROWTH The growth and survival of microorganisms are affected by the chemical and physical conditions of the external environment. Environmental factors which have significant
More informationContribution of Oxidative Enzymes to the Degradation of Cellulose by Filamentous Fungi. William Templeton Beeson IV
Contribution of Oxidative Enzymes to the Degradation of Cellulose by Filamentous Fungi by William Templeton Beeson IV A dissertation in submitted in partial satisfaction of the requirements for the degree
More informationProduction of Biofuels AO Olaniran
Production of Biofuels AO Olaniran Department Microbiology University of KwaZulu- Natal (Westville Campus) Microorganisms may be used to convert waste products, plants, or microbial biomass into liquid
More informationAspergillus oryzae. Applied and Environmental Microbiology, May 2006, p , Vol. 72, No. 5
Aspergillus oryzae Applied and Environmental Microbiology, May 2006, p. 3448-3457, Vol. 72, No. 5 Speaker: Chia-wen Chen Advisor: Huang, C.-T 2007/10/30 1 Aspergillus oryzae Kingdom Phylum Subphylum Class
More informationM I C R O B I O L O G Y
ninth edition TORTORA FUNKE CASE M I C R O B I O L O G Y a n i n t r o d u c t i o n 6 Microbial Growth PowerPoint Lecture Slide Presentation prepared by Christine L. Case Microbial Growth Microbial growth
More informationImportance. Prokaryotes vs. Eukaryotes. Viruses: a form of life or not?
1 Importance Microorganisms (esp. bacteria) plays a key role in the decomposition and stabilization of organic matter Control of diseases caused by pathogenic organisms of human origin Prokaryotes vs.
More informationBRITISH BIOMEDICAL BULLETIN
Journal Home Page www.bbbulletin.org BRITISH BIOMEDICAL BULLETIN Original Bioinformatics Analysis of α-amylase Three-Dimensional Structure in Aspergillus oryzae Javad Sharifi-Rad,2, Fatemeh Taktaz* 3 and
More informationThermogladius calderae gen. nov., sp. nov., an anaerobic, hyperthermophilic crenarchaeote from a Kamchatka hot spring
International Journal of Systematic and Evolutionary Microbiology (2016), 66, 1407 1412 DOI 10.1099/ijsem.0.000916 Thermogladius calderae gen. nov., sp. nov., an anaerobic, hyperthermophilic crenarchaeote
More informationInternational Journal of Advanced Research in Biological Sciences ISSN : Research Article
International Journal of Advanced Research in Biological Sciences ISSN : 2348-8069 www.ijarbs.com Research Article Isolation and characterization of thermophilic bacteria of a hot water spring source,
More informationWhole genome sequencing of butyrateproducing
Whole genome sequencing of butyrateproducing bacteria Jakub Mrazek, Francesco Strozzi, Katerina Fliegerova, Jiri Simunek, Hana Sechovcova, Diego Grilli, Jan Kopecny IAPG CAS, Prague, Czech Republic Budapest,
More informationResearch Article A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans
Archaea Volume 15, Article ID 978632, 6 pages http://dx.doi.org/10.1155/15/978632 Research Article A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans
More informationchondroitin sulfate (Td max. ~42 h) laminarin (Td max. ~47 h) mannose (Td max. ~34 h) time [h]
.4 chondroitin sulfate (Td max. ~42 h) laminarin (Td max. ~47 h) mannose (Td max. ~34 h) optical density (6 nm).3.2.1. 5 1 15 2 25 time [h] Supplementary Figure S1 Growth curves of Polaribacter sp. Hel1_33_49.
More informationResearch Article PRODUCTION OF A THERMOSTABLE EXTRACELLULAR PROTEASE FROM THERMOPHILIC BACILLUS SPECIES
Research Article PRODUCTION OF A THERMOSTABLE EXTRACELLULAR PROTEASE FROM THERMOPHILIC BACILLUS SPECIES S. Suman *1 and K. Ramesh 1 1 Department of Pharmaceutical Biotechnology, Karnataka College of Pharmacy,
More informationGenomic and proteomic approaches to explore the diversity of cellwall-degrading enzymes
Genomic and proteomic approaches to explore the diversity of cellwall-degrading enzymes Rendez-Vous Protéomique 2009 April 16, 2009 Adrian Tsang Concordia University The future Graphics obtained from U.S.
More informationTranscriptomic analysis of lignocellulosic biomass degradation by the anaerobic fungal isolate Orpinomyces sp. strain C1A
DOI 10.1186/s13068-015-0390-0 Biotechnology for Biofuels RESEARCH Transcriptomic analysis of lignocellulosic biomass degradation by the anaerobic fungal isolate Orpinomyces sp. strain C1A M. B. Couger
More informationFaculty Mentor, Department of Microbiology and Molecular Genetics, Oklahoma State University
Mapping Microbial Diversity of Culturable Strains in Oklahoma Authors: Abigail Grathwohl, Casey Johnson, Samantha Shafer, and Dr. Wouter Hoff * Abstract: Understanding the bacterial community that surrounds
More informationBiodiversity of Thermophilic Prokaryotes with Hydrolytic Activities in Hot Springs of Uzon Caldera, Kamchatka (Russia)
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jan. 2009, p. 286 291 Vol. 75, No. 1 0099-2240/09/$08.00 0 doi:10.1128/aem.00607-08 Copyright 2009, American Society for Microbiology. All Rights Reserved. Biodiversity
More informationModeling of Anaerobic Digestion of Sludge
Engineering Conferences International ECI Digital Archives Wastewater and Biosolids Treatment and Reuse: Bridging Modeling and Experimental Studies Proceedings Spring 6-8-2014 Modeling of Anaerobic Digestion
More informationABSTRACT. agricultural waste, non-edible parts of crops, and grasses into fermentable sugars.
ABSTRACT OZDEMIR, INCI. Role of S-layer Homology Domain Proteins in Plant Biomass Conversion to Biofuels by Extremely Thermophilic Caldicellulosiruptor species. (Under the direction of Robert M. Kelly.)
More information1. ADHERE AND DEFEND: Our bacterium has entered the host. Now it needs to adhere and get past the normal microbiota.
North Seattle College Stage 02 Colonization and Infection This explanatory model will tell the story of how one bacterium adheres to a host and, through binary fission, ends up making two daughter cells.
More informationEMPOWERING BIOINNOVATION BIO PACIFIC RIM SAN DIEGO 12/9/2014 NAGIB M. WARD, EXECUTIVE VP
EMPOWERING BIOINNOVATION BIO PACIFIC RIM SAN DIEGO 12/9/2014 NAGIB M. WARD, EXECUTIVE VP DEINOVE CREATES RENEWABLE, RESPONSIBLE AND COST-EFFECTIVE INDUSTRIAL PROCESSES OUR AMBITION Replace petroleum-based
More informationThe HotZyme Newsletter
Issue 01 2015 The HotZyme Newsletter SYSTEMATIC SCREENING FOR NOVEL HYDROLASES FROM HOT ENVIRONMENTS From the Editor Dear readers, Welcome to the 13th and the last edition of the HotZyme Newsletter. HotZyme
More informationBiological Wastewater Denitrification by Thermophilic Bacteria
Yakhlef et al. 147 Journal Academica Vol. 2(3), pp. 147-151, September 29 2012 - Microbiology - ISSN 2161-3338 online edition www.journalacademica.org 2012 Journal Academica Foundation Full Length Research
More informationNorth American Consortium for Genomics of Fibrolytic Ruminal Bacteria
North American Consortium for Genomics of Fibrolytic Ruminal Bacteria Ohio State University (Lead Institution) Mark Morrison The Institute for Genomics Research (TIGR) Karen Nelson, Sean Daugherty, Bill
More informationClassification of Microorganisms
PowerPoint Lecture Presentations prepared by Bradley W. Christian, McLennan Community College C H A P T E R 10 Classification of Microorganisms The Study of Phylogenetic Relationships Taxonomy is the science
More informationIncreasing Protein Stability
Increasing Protein Stability Stability depends on the extent of disulfide bond the presence of certain amino acids at the N terminus (Amino acids added to N terminus of β-galactosidase) (Table 6.4) Met,
More informationECO Ultra 10X Concentrate
ECO Ultra 10X Concentrate A superior Multi-Action Microbial Consortium MULTI-ACTION MICROBIAL CONSORTIUM Eco Ultra concentrate microbial consortium demonstrates superior enzyme performance for use in multiple
More informationPROCESS OPTIMIZATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE ENZYME FROM BACILLUS ALTITUDINIS (MCCB 0014)
Academic Sciences International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 975-1491 Vol 4, Issue 3, 212 Research Article PROCESS OPTIMIZATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE
More informationIntroduction to Industrial Biotechnology. Lecture 5 Consolidated bioprocessing
Introduction to Industrial Biotechnology Lecture 5 Consolidated bioprocessing Learning outcomes Understand the costs of an cellulosic biofuel plant Appreciate the concept of consolidated bioprocessing.
More informationMOLECULAR IDENTIFICATION OF THE MOST COMMON CELLULASE PRODUCING-BACILLUS FROM FOREST SOILS
: 2250-2256 ISSN: 2277 4998 MOLECULAR IDENTIFICATION OF THE MOST COMMON CELLULASE PRODUCING-BACILLUS FROM FOREST SOILS TOULAMI S 1, JORSHARI S 1, FAZELTOLAMI H 2, PAST S 3, SEYED ALI ROTEH SM 4, ASOUDEHMOGHANLOO
More informationA novel biocatalyst for Cellulosic Ethanol Production
University of Massachusetts Amherst ScholarWorks@UMass Amherst Conference on Cellulosic Biofuels September 2008 A novel biocatalyst for Cellulosic Ethanol Production Susan Leschine UMass Amherst, suel@microbio.umass.edu
More informationInsights into high-efficiency lignocellulolytic enzyme production by Penicillium oxalicum GZ-2 induced by a complex substrate
Liao et al. Biotechnology for Biofuels 2014, 7:162 RESEARCH Open Access Insights into high-efficiency lignocellulolytic enzyme production by Penicillium oxalicum GZ-2 induced by a complex substrate Hanpeng
More informationComparison of aerobic and anaerobic cellulose degrading microorganisms in sediments of Sippewissett Salt March
Comparison of aerobic and anaerobic cellulose degrading microorganisms in sediments of Sippewissett Salt March Victoria Grießmeier Karlsruhe Institute of Technology (KIT) MBL Microbial Diversity Course
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(4):498-503 Optimization of culture conditions for Keratinase
More informationMicrobial Biotechnology agustin krisna wardani
Microbial Biotechnology agustin krisna wardani 1. The Structure of Microbes Microbes (microorganisms) are tiny organisms that are too small to be seen individually by the naked eye and must be viewed with
More informationTable of Contents 1. CHAPTER I INTRODUCTION 1 [A] GLUCOSE ISOMERASE 1
Table of Contents 1. CHAPTER I INTRODUCTION 1 [A] GLUCOSE ISOMERASE 1 1.1 Enzymatic versus chemical isomerization 4 1.2 Glucose Isomerase producing microorganisms 5 1.3 Structure of Glucose Isomerase 8
More informationSupplementary Figure Legends
Supplementary Figure Legends Figure S1. A) Proportion of different functional C-groups of soil organic matter in one control and two burn locations. Error bars indicate the standard error of mean. B) Indices
More informationECO FFWD Concentrate 10X
ECO FFWD Concentrate 10X A superior biological treatment for cleaning & waste applications MULTIACTION MICROBIAL CONSORTIUM Eco FFWD Concentrate s microbial consortium demonstrates superior enzyme performance
More informationVALORPLUS: VALORISING BIOREFINERY BY-PRODUCTS. FP7 EC KBBE-CALL 7- Project No
VALORPLUS: VALORISING BIOREFINERY BY-PRODUCTS FP7 EC KBBE-CALL 7- Project No. 613802 VALORPLUS: VALORISING BIOREFINERY BY-PRODUCTS Valorisation of biorefinery by-products leading to closed loop systems
More informationNext Generation Sequencing Techniques in Food Microbiology Remco Kort, Professor,
Next Generation Sequencing Techniques in Food Microbiology Remco Kort, Professor, Microbiology & Systems Biology, Netherlands Institute for Applied Scientific Research (TNO) Microbe-Host Interactions VU
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:10.1038/nature12212 Supplementary Discussion Contamination Assessment We evaluated the amount of human contamination in our viral DNA preparations by identifying sequences
More informationInvestigation of anaerobic digestion in a two-stage bioprocess producing hydrogen and methane
15 th European biosolids and organic resources, Leeds, Nov. 2010 : Two-stage anaerobic digestion S. Hiligsmann 1 15 th European biosolids and organic resources Leeds 15-17 th nov. 2010 Investigation of
More informationBacterial Requirements. Growth and Nutrition
Bacterial Requirements Growth and Nutrition Bacterial Reproduction Reproduction Binary Fission Budding Fragmenting Function Increase number of cells Genetic recombination possible End result : Growth Generation
More information"From the Investigation of marine model species to biotechnological developments, a knowledgebased
"From the Investigation of marine model species to biotechnological developments, a knowledgebased approach" Catherine Boyen OECD Global Forum on Biotechnology: Marine Biotechnology Enabling Solutions
More informationExecutive Summary New Energy Company of Indiana CRADA Completed 1997 Public Release 1999
Executive Summary New Energy Company of Indiana CRADA Completed 1997 Public Release 1999 CRADA Background The CRADA between the National Renewable Energy Laboratory and the New Energy Company of Indiana
More informationIndex. Index 419. B Bacillus mojavensis, Bacterial cells, 14, 17, 21, 103, 104, 147, 182, 183, 214, 354, 360, 370, 398 isolation, 14, 370
Index 419 Index 13 C NMR, in vivo, 173 175, 183 186, 396, 402 16S rdna, 3 8, 25, 30, 31, 56 58, 159, 160, 235, 412 amplification, 23 27, 62, 78 81, 237 240, 325, 407, 408, 411 library construction, 78
More informationBiogas Production Microbial Teamwork and Enzymology
Biogas Production Microbial Teamwork and Enzymology Live H. Hagen KBM, Norwegian University of Life Sciences (NMBU), Ås, Norway Contact: live.hagen@nmbu.no CenBio Final Conference Ås Campus 13-14 March
More informationInt.J.Curr.Res.Aca.Rev.2016; 4(3): 39-47
Biotechnological Applications of Hyperthermophilic Enzymes Raj Kumar Pandey 1 *, Anupam Barh 2, Dinesh Chandra 3, Satish Chandra 3, Vishakha Pandey 4, Pankaj 1 and Lakshmi Tewari 1 1 Department of Microbiology,
More informationAnswers to Module 1. An obligate aerobe is an organism that has an absolute requirement of oxygen for growth.
Answers to Module 1 Short Answers 1) What is an obligate aerobe? An obligate aerobe is an organism that has an absolute requirement of oxygen for growth. What about facultative anaerobe? 2) Distinguish
More informationScreening and selection of a novel microbe for esterase production
Chapter 3 Screening and selection of a novel microbe for esterase production 3.1. Introduction Microorganisms can be considered as a treasure trove of useful enzymes. Majority of biologically relevant
More informationActivity 2.3 Life sciences, biotechnologies and biochemistry for sustainable non-food products and processes
Activity 2.3 Life sciences, biotechnologies and biochemistry for sustainable non-food products and processes Presentación Tema 2 FP7 CDTI, Madrid 11 de mayo de 2011 INFO-DAY KBBE Bruxelles, 15 July 2011
More informationModule F06FB08. To gain knowledge about enzyme technology and production of enzymes and
Module F06FB08 Enzyme technology Introduction and Production of enzymes This module would focus on enzyme technology which deals with the enzymes, the metabolic catalysts and their use in various Industries.
More informationChapter 6: Microbial Growth
Chapter 6: Microbial Growth 1. Requirements for Growth 2. Culturing Microorganisms 3. Patterns of Microbial Growth 1. Requirements for Growth Factors that affect Microbial Growth Microbial growth depends
More informationInstant download and all chapter of Test bank for Microbiology An Introduction 12th Edition by Tortora
Instant download and all chapter of Test bank for Microbiology An Introduction 12th Edition by Tortora Link download full: http://testbankair.com/download/test-bank-for-microbiology-anintroduction-12th-edition-by-tortora/
More informationISOLATION AND MOLECULAR CHARACTERIZATION OF THERMOTOLERANT BACTERIA FROM MANIKARAN THERMAL SPRING OF HIMACHAL PRADESH, INDIA ABSTRACT
ISOLATION AND MOLECULAR CHARACTERIZATION OF THERMOTOLERANT BACTERIA FROM MANIKARAN THERMAL SPRING OF HIMACHAL PRADESH, INDIA Ambika Verma 1*, & Poonam Shirkot 2 1*Ph.D Scholar, Department of Biotechnology,
More informationENZYME TECHNOLOGY TO DRIVE PROFITS IN BIOGAS PRODUCTION Sandra Hinz, PhD, DuPont Industrial Biosciences 5 July 2017
ENZYME TECHNOLOGY TO DRIVE PROFITS IN BIOGAS PRODUCTION Sandra Hinz, PhD, DuPont Industrial Biosciences 5 July 2017 TWO EARLY PIONEERS IN MODERN INDUSTRIAL BIOTECHNOLOGY Genencor Genentech & Corning form
More informationBioconversion of agricultural residues and bio-based waste streams
Bioconversion of agricultural residues and bio-based waste streams DST Science-meets-Industry Workshop: Organic waste 26-27 November 2014 Brett Pletschke Rhodes University Biobased economy, Bioeconomy,
More informationEnzymatic Activity of Marine Lactobacillus Species from South East Coast of India.
Enzymatic Activity of Marine Lactobacillus Species from South East Coast of India. Vinola Jennifer and Govindasamy Thiruneelakandan, Department of Microbiology, Srimad Andavan Arts and Science College,
More informationBench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems
Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems Ramchuran, Santosh Published: 2005-01-01 Link to publication Citation for
More informationBrief summary of prepgem and forensicgem Properties. ZyGEM: Sept
Brief summary of prepgem and forensicgem Properties ZyGEM: Sept. 2007. SUMMARY OF forensicgem AND prepgem PROPERTIES Enzyme Description and purity The key component of forensicgem and prepgem is a neutral
More informationBiotechnology & Biofuels. Dr. Simon K.-M. R. Rittmann
Biotechnology & Biofuels Dr. Simon K.-M. R. Rittmann What is Biotechnology? Ø Any technological application using biological systems, living organisms, or derivatives thereof, to manufacture or modify
More informationORFs, unknown function GC (%) # of ORFs. Fen-75cm Fn (91) Nitrosopumilus maritimus
Table S1 Comparison of genome properties for 10 distinct bins identified from two metagenomes. The mean coverage is calculated by dividing total read counts with read length. ORF, open reading frame. Nearest
More informationFigure S1. Phylogenetic tree comparing c-type cytochrome proteins from Ferroglobus placidus to c-type cytochromes from other bacterial and archaeal
Fiure S1. Phyloenetic tree comparin c-type cytochrome proteins from Ferrolobus placidus to c-type cytochromes from other bacterial and archaeal species. 1 Fiure S2. Alternative complex III operons found
More informationCONTENTS VOLUME IX. Marine Biotechnology 1 Indrani Karunasagar, University of Agricultural Sciences, India
CONTENTS VOLUME IX Marine Biotechnology 1 Indrani Karunasagar, University of Agricultural Sciences, India 1. Scope of Marine Biotechnology 2. Industries Based on Marine Biotechnology 3. Scientific Studies
More informationMicrobial communities in restored freshwater wetlands. Susannah Green Tringe DOE Joint Genome Institute INTECOL, June 5, 2012
Microbial communities in restored freshwater wetlands Susannah Green Tringe DOE Joint Genome Institute INTECOL, June 5, 2012 Talk outline Project motivation and background Metagenomics and carbon cycling
More informationProduction Of Cellulase From Corn Cobs By Aspergillus niger Under Submerged Fermentation
International Journal of ChemTech Research CODEN (USA): IJCRGG ISSN : 0974-4290 Vol.6, No.5, pp 2900-2904, Aug-Sept 2014 Production Of Cellulase From Corn Cobs By Aspergillus niger Under Submerged Fermentation
More informationIndustrial Application of Biotechnology
Industrial Application of Biotechnology 2004 Seungwook Kim Chem. & Bio. Eng. WJ Thieman and MA Palladino, Introduction to Biotechnology(2004), Pearson. Reference SR Barnum, Biotechnology;An Introduction(1998),
More informationInt. J. Pharm. Sci. Rev. Res., 32(2), May June 2015; Article No. 26, Pages:
Research Article Enhanced Production of Cellulase on Different Fruit Peel Under Submerged Fermentation M. Kannahi*, S. Elangeswari PG and Research Department of Microbiology, Sengamala Thayaar Educational
More informationАquaphotomics in biotechnology - new tools and opportunities
Аquaphotomics in biotechnology - new tools and opportunities Prof. Albert Krastanov UNIVERSITY OF FOOD TECHNOLOGIES AQUAPHOTOMICS time-efficient allows rapid, chemical-free, noninvasive in vivo assessment
More informationSafety evaluation of technical enzyme products. legislation. 09 June 2009 Brussels
Safety evaluation of technical enzyme products with regards to the REACH legislation 09 June 2009 Brussels ENZYMES What enzymes are Enzymes are proteins with highly specialized catalytic function Produced
More informationENVE 424 Anaerobic Treatment. Review Lecture Fall Assist. Prof. A. Evren Tugtas
ENVE 424 Anaerobic Treatment Review Lecture 2012-2013 Fall Assist. Prof. A. Evren Tugtas Basics of Microbiology Principles of microbiology is applied to the solution of environmental problems Treatment
More informationIntegrative Biorefinery
Integrative Biorefinery research@ibl Conversion of renewable plant biomass to fuels and chemicals: Bio-process Verawat Champreda, Ph.D Enzyme Technology Laboratory Integrative Biorefinery Laboratory National
More informationMicrobially Mediated Plant Salt Tolerance and Microbiome based Solutions for Saline Agriculture
Microbially Mediated Plant Salt Tolerance and Microbiome based Solutions for Saline Agriculture Contents Introduction Abiotic Tolerance Approaches Reasons for failure Roots, microorganisms and soil-interaction
More informationTable 4.1 Linnaean Classification System of Organisms.
Table.1 Linnaean Classification System of rganisms. Domain Kingdom Phylum Class rder Family Genus Species Table.2 Comparison of prokaryotic and eukaryotic cells. Characteristic Prokaryotic Eukaryotic Cell
More informationCTB3365x Introduction to Water Treatment
CTB3365x Introduction to Water Treatment W3a Oxidation fundamentals and kinetics Merle de Kreuk Do you already question yourself how soluble components are removed from sewage? And what the brown suspension
More informationHigh-level Expression and Characterization of Thermostable Esterase from Thermoanaerobacter Tengcongensis in Escherichia coli
High-level Expression and Characterization of Thermostable Esterase from Thermoanaerobacter Tengcongensis in Escherichia coli Ruobing Deng, Xun Li, Hong Gao, Fei Wang China College of Chemical Engineering,
More informationExtracellular Amylase Activity from Endophytic Streptomyces griseoflavus P4
346 Chiang Mai J. Sci. 2012; 39(2) Chiang Mai J. Sci. 2012; 39(2) : 346-350 http://it.science.cmu.ac.th/ejournal/ Short Communication Extracellular Amylase Activity from Endophytic Streptomyces griseoflavus
More informationSome Industrially Important Microbes and Their Products
2 Some Industrially Important Microbes and Their Products 2.1. ENZYME PRODUCING MICROBES Type of enzyme Substrate Microorganism Amylase Starch Saccharomyces diastaticus Protease Proteins Bacillus sp. Lipase
More informationEnzymes and biotechnology: could we overcome modern challenges? 2nd International Conference on Genomics & Pharmacogenomics
Enzymes and biotechnology: could we overcome modern challenges? 2nd International Conference on Genomics & Pharmacogenomics Dr. Junio Cota VTT Brasil LTDA 2 SUMMARY What is VTT? The OMICS Era New Enzyme
More informationUMWEKO GmbH, Dr Konrad Schleiss
3. Biology of anaerobic digestion (after Peter Weiland, Institute of Technology and Biosystems Engineering Federal Agricultural Research Centre, Braunschweig, Germany) Microbiology and biochemistry of
More informationEnzymes and Cellular Regulation
Why? Enzymes and Cellular Regulation What are the factors that regulate the rate at which enzymes catalyze reactions? Digestive enzymes are protein-based biological catalysts that play important roles
More informationIntroduction to BIOFUELS. David M. Mousdale. CRC Press. Taylor & Francis Group Boca Raton London New York
Introduction to BIOFUELS David M. Mousdale CRC Press Taylor & Francis Group Boca Raton London New York CRC Press is an imprint of the Taylor & Francis Croup, an informa business Contents Preface Acknowledgments
More informationGROUND-WATER MICROBIOLOGY AND GEOCHEMISTRY
J8Q5-900 GROUND-WATER MICROBIOLOGY AND GEOCHEMISTRY Second Edition Francis H. Chapelle United States Geological Survey JOHN WILEY & SONS, INC. New York / Chichester / Weinheim / Brisbane / Singapore /
More informationMarine meta/genomic libraries: establishing resources for MAMBA project
Marine meta/genomic libraries: establishing resources for MAMBA project about 70% of the Earth surface marine environments Marine animals Microorganisms (Yakimov et al., 2004) Deep-sea corals Desmophilum
More informationVectors for Gene Cloning: Plasmids and Bacteriophages
Vectors for Gene Cloning: Plasmids and Bacteriophages DNA molecule must be able to replicate within the host cell to be able to act as a vector for gene cloning, so that numerous copies of the recombinant
More informationMICROBIAL BIOPROSPECTING
MICROBIAL BIOPROSPECTING MICROBIAL BIOPROSPECTING Brazil, due to its large diversity of biomes and ecosystems, its large territorial extension, and its location between the tropics, where the planet s
More informationProduction and purification of enzyme Xylanase by Aspergillus niger
ISSN: 2319-7706 Volume 3 Number 10 (2014) pp. 664-668 http://www.ijcmas.com Original Research Article Production and purification of enzyme Xylanase by Aspergillus niger S.Subbulakshmi and Priya R Iyer*
More informationProduction and characterization of cellulolytic enzymes by Pleurotus florida
Production and characterization of cellulolytic enzymes by Pleurotus florida Mycosphere Goyal M * and Soni G Department of Biochemistry, Punjab Agricultural University, Ludhiana-141004. Punjab, India Goyal
More informationPRODUCTION OF CELLULOLYTIC ENZYMES PROCESS WITH TRICHODERMA ATROVIRIDE MUTANTS FOR THE BIOMASS-TO-BIOETHANOL. Krisztina Kovács
PRODUCTION OF CELLULOLYTIC ENZYMES WITH TRICHODERMA ATROVIRIDE MUTANTS FOR THE BIOMASS-TO-BIOETHANOL PROCESS Cellulase Production, Enzymatic Hydrolysis and Simultaneous Saccharification and Fermentation
More informationShewanella violacea. Pressure regulation of quinol oxidase expression in Piezophilic. M. Hassan Qureshi
Shewanella violacea M. Hassan Qureshi Moderately Shewanella violacea o c S. violacea cyoa cyob cyoc cyod cyoe S. violacea Shewanella violacea Pressure regulation of quinol oxidase expression in Piezophilic
More information14 th Lecture Biogas and Biohydrogen
Biotechnology and Energy Conservation Prof. Dr.oec.troph. Ir. Krishna Purnawan Candra, M.S. Program Magister Ilmu Lingkungan Universitas Mulawarman 14 th Lecture Biogas and Biohydrogen The Aim: Students
More informationProf. Arkady P.Sinitsyn
Enzyme complex for highly efficient saccharification of lignocellulosic feedstocks Prof. Arkady P.Sinitsyn A.N.Bach Institute of Biochemistry, Russian Academy of Sciences German-Russian Forum Biotechnology
More information