Bacillus subtilis as an Alternative Source of Beta-glucosidase

Size: px
Start display at page:

Download "Bacillus subtilis as an Alternative Source of Beta-glucosidase"

Transcription

1 Article International Journal of Modern Cellular and Molecular Biology, 2014, 3(1): 1-9 International Journal of Modern Cellular and Molecular Biology Journal homepage: ISSN: Florida, USA Bacillus subtilis as an Alternative Source of Beta-glucosidase Bagudo, A. I 1*, A. U. Argungu 2, Aliero, A. A 1., S 1., Suleiman, N., 3 Kalpana, S. 1 1 Microbiology Unit, Department of Biological Sciences, Kebbi State University of Science and Technology, Aliero, Nigeria. 2 Department of Biochemistry, Kebbi State University of Science and Technology, Aliero, Nigeria. 3 Department of Veterinary Biochemistry Usmanu Danfodiyo University Sokoto, Nigeria. * Author to whom correspondence should be addressed; aamadee96@gmail.com, Article history: Received 22 November 2013, Received in revised form 27 December 2013, Accepted 16 January 2014, Published Abstract: Bacillus species of soil origin are known to produce a wide variety of extracellular enzymes which are of vast agricultural and industrial uses. One gram (1g) each of soil obtained from a local farm lands in Aliero, Kebbi State was tenfold serially diluted then 0.1ml of 10 5 and 10 6 dilutions was inoculated into primary media followed by inoculation in to production media. The bacterial isolate was identified as Bacillus subtilis which was used in this study for the production of Beta-glucosidase. The enzyme activity was determined using Dinitrosalicylic (DNS) method. Highest production by B. subtilis was obtained following ten(10) hours of incubation. The optimum temperature for the activity of Betaglucosidase produced was obtained at 60 C with a concentration of 0.55 mg/ml. Optimum ph was obtained at ph 7.0 with a concentration of 0.67 mg/ml. The results of this study revealed that B. subtilis is a potential source of extracellular Beta-glucosidase at high temperatures suitable for industrial uses. Keywords: Farm lands, Bacillus subtilis, Beta-glucosidase, Industrial applications

2 2 1. Introduction Huge amount of agricultural, domestics and industrial cellulosic wastes have been accumulating and are continuously deposited into our environment. Celluloses are considered as the most important renewable resource for bioconversion (Kapdan et al., 2006). Many Cellulosic substances were hydrolyzed to simple sugars for making Single Cell Protein, sweeteners etc. It has been become the economic interest to develop an effective method to hydrolyze the cellulosic biomass (Peng et al., 2011). One of the important sources for a number of extracellular enzymes is soil microorganisms. Soils, rice straw, wheat straw and naturally decaying organic matters that contain cellulose materials are the best sources to isolate these microorganisms. A number of bacteria have been reported for their cellulolytic activities. The type of extracellular enzyme formed may depend on the composition of the medium and culture conditions; therefore selection of appropriate organism for a particular enzyme plays a key role in high productivity of desirable enzyme (Christakopoulos et al., 1994). The genus Bacillus contains a number of industrially important species and at present approximately half of the industrial productions of the enzymes are obtained from the strains of Bacillus sp. These strains are specific producers of extracellular cellulases and can be cultivated under high temperature and at various ph conditions to give rise to products that are in turn, stable in a wide range of harsh environments (Chen et. al., 2006). Cellulase is composed of several enzymes complex which functions together to hydrolyze cellulose to its sub-units component i.e. glucose. Cellulases produced by some bacteria tend to be cell associated and form tight multi enzyme complexes called cellulosome, on the surface of the cell. Cellulosome exhibits a cellulose binding function that allows the bacterial cell to bind closely to its substrate. Cellulosome is microcellular machine, whose component interacts in a synergistic manner to catalyze the efficient degradation of cellulose (Bayer, et al., (2007).). The enzyme has great economic significance. An efficient cellobiose hydrolysis requires a large amount of Beta-glucosidase for the utilization of cellulose residues, on an industrial scale. Beta-glucosidases are heterogeneous enzymes that have aroused considerable interest primarily because of their involvement in the biological saccharification of cellulosic material. For industrial use, isolation and characterization of new high yielding strains using less costly carbon source still remains a challenge (Zhang et. al., 2006). The aim of this work is therefore to identify bacterial as a source of Beta-glucosidase for industrial applications. 2. Materials and Methods 2.1. Sample Collection and Processing Soil samples collected from different cultivations farms lands in Aliero local Government, Kebbi State, Nigeria for screening of Bacillus species producing Beta-glucosidase were brought to

3 3 Microbiology laboratory, Kebbi State University of Sciences and Technology, Aliero. Soil samples were heat-shocked briefly at 80 o C for 15 minutes to kill vegetative cells and non-spore former (Saraswati et al., 2012). Serial dilutions of each soil sample were made in sterilized distilled water and 0.1 ml of 10-5 and 10 6 dilutions of each sample was spread on the surface of modified Han s medium containing (Yeast extract 2g/L, Cellulose 5g/L, Peptone 2g/L and Agar 15g/L). The plates were flooded with Congo red to see the cellulolytic activity of isolated strain after incubated at 35 o C for 48 h Cellulase producing microorganism showed a zone of clearance on this agar (Christakopoulos,et al., 1994), figures 1 and 2. Figure 1. showing plate 10 5 dilutions Figure 2. showing plate 10 6 dilutions 2.2. Identification of Bacillus The selected isolates were identified to the genus level using morphological and biochemical methods. Morphological examination was observed by a light microscope and Gram strain. The conventional biochemical tests used for identification of Bacillus are motility, Voges-Proskauer, Nitrate reduction, acid and/gas production from glucose, Mannitol fermentation, Hydrolysis of (Starch, Gelatin or Tyrosine), Casein Hydrolysis, Catalase test and Citrate utilization test and Propionate utilization (Bergey s Manual, (2000) Identification of Cellulase Producing Bacillus The colonies showing clear zone were sub-cultured and re-streaked on the first medium again to have pure cultures. The purified isolates were preserved at - 80 o C in glycerol solution and kept on slants at 4 o C for further study. 100 ml of autoclaved production medium (Second medium) with following composition: MgSO4.7H2O 0.3g/L, NH4SO4 1.0g/L, K2HPO4, 3.6g/L, Na2HPO4. 12H2O 18.8g/L, NaCl 0.4g/L and Sucrose 10.0g/L was inoculated with 1 ml of test culture and incubated in rotary shaker at 20, 000 rpm at 37 C for 72 hours (Kalaichelvan, et al., (2012) Extraction of Enzyme from Bacteria

4 4 The selected isolates were incubated at 37 C for 24 h in 50 ml of 8% (w/v) starch medium in 250 ml conical flask and placed in a shaker incubator operated at 120 rpm at 30 C. The extracellular enzyme solutions were obtained by centrifugation at 5000 rpm for 20 min using a high speed centrifuge. The supernatant obtained was collected and used as enzyme source and used in enzyme assays (Singh et al., 1998) Characterization of the Enzyme Measurement of optimum ph for enzyme activity ph stability of the enzyme was determined by the incubation of enzyme for 30 minutes at room temperature with buffer solutions of varying ph range. The ph range of the phosphate buffers used was of ph The residual activity was then determined at 600C under standard assay conditions (Liu, et al., 2008; and Qu, et al., 2008) Measurement of optimum period for enzyme production The effect of time course on cells growth and Beta-glucosidase production were determined by measuring the absorbance of cells growth and determining the cellulase activity at different time intervals. The results indicated that maximum Beta-glucosidase activity was observed after 10 hours of fermentation with sucrose as a carbon source. After 10 hours a decline in growth and Beta-glucosidase activity was observed (Maki et al., 2009) Measurement of optimum temperature for enzyme activity The optimum temperature stability of the enzyme was measured by the incubation of enzyme in 0.05M phosphate buffer of ph 7.0 for 2 hour at temperature ranging from o C. The residual activity was then determined at 60 O C using standard assay conditions (Lo et al., 2009; and Bai et al., 2009) Measurement of Beta-glucosidase-activity Measurement of the amount of reducing sugars released by dinitro-salicyclic acid (DNS) was used to assay the activity of beta-glucosidase. 0.5 ml, each of salicyclic acid and diluted enzymes respectively and 0.05 M phosphate buffer at ph 7.0 was mixed in a test tube and incubated at 60 o C for 10 minutes and this is followed by the addition of 3 ml of DNS reagent to the enzyme mixture and heated in water bath at 100 o C for 15 minutes and 0.5 ml of salicin, 3.0 ml DNS and 0.5 ml of the enzyme of the same dilution was used as a reference blank to estimate the reducing sugars. The mixture was then placed in boiling water bath for 15 minutes and cooled to room temperature. The absorbance was then measured at 600 nm. The absorbance of the sample corrected by subtraction of enzyme blank was used to calculate glucose concentration from the glucose standard curve (Mallier 1959).

5 5 3. Results The appearance of white-creamy and dry colonies was indicative of the growth of Bacillus-like bacteria and number of colonies ranged from to CFU g 1 and on gram staining, gram positive, motile, Voges-poskrauer negative, Nitrate reduction positive, acid and gas production from Glucose,, Starch Hydrolysis and Gelatin Hydrolysis positive rods were observed. The effect of temperature on the cell growth and Beta-glucosidase production was studied by growing the organism at different temperature. As fermentation temperature was increased from 40ºC to 65ºC, cell growth and Beta-glucosidase activity in the culture supernatant gradually increased from 0.50 U/mL to 1.72 U/mL (table 1). After 60ºC, the cell growth and Beta-glucosidase activity gradually decrease. Since the maximum Beta-glucosidase activity was observed at 60ºC, this temperature was used in the subsequent experiments to determine the effect of other parameters for growth and Beta-glucosidase production. Table 1: Measurement of optimum temperature for Cells growth and enzyme production Temperature (ºC) Cells growth (O/D) Beta-glucosidase activity u/ml The effect of initial ph of the culture medium on the cell growth and Beta-glucosidase production was studied by growing the organism at different ph values ranging from 4.5 to 8.5 at 60ºC. As the ph of the culture medium increased from 4.5 to 7.0, Beta-glucosidase activity increased from to U/mL. The maximum cell growth was measured at ph 7.0. Increasing the ph to 8.5, the Betaglucosidase activity was reduced to U/mL. Since maximum Beta-glucosidase activity, again this ph 7.0 was chose, so the ph of the medium was selected as 7.0 (table 2). Different carbon sources on cell growth and Beta-glucosidase production was studied, a number of carbon sources at a concentration of 0.5% were used. The maximum yield of Beta-glucosidase activity was recorded after 10 hours of fermentation when sucrose was used as a carbon source. Sucrose was the best amongst the carbon sources i.e. glucose, lactose, cellobiose and avicel. The maximum Betaglucosidase activity was U/mL, when sucrose was used as the carbon source (table 3). The

6 6 minimum cell growth and Beta-glucosidase activity was observed when avicel was used as carbon source. Therefore, sucrose was selected as the carbon source for the production of Beta-glucosidase. Table 2: Measurement of optimum ph for Cells growth and enzyme production Medium ph Cells growth (O/D) Beta-glucosidase activity U/mL Table 3: Effects of different carbon source for cells growth and enzyme production Carbon source Cells growth (O/D) Beta-glucosidase activity U/mL Glucose Sucrose Lactose Cellulobiose Avicel Discussion Production and partial characterization of Beta-glucosidase by Bacillus subtilis isolated from a local farm lands was investigated. Bacillus spp forms one of the major soil saprophytes as reported by Olajuyigbe et al. (2005) who identified twenty five bacterial isolates from soil of which nine were identified as Bacillus species. The occurrence of cellulolytic organisms in soil agrees with earlier reports of (Rehena et al., 1989) that soil is known to be a reserve of Bacillu sp producing Beta-glucosidase. The effect of incubation period on growth and Beta-glucosidase production by B. subtilis revealed that

7 7 production begins to increase from six hours (6hrs) to peak at ten hours (10hrs) at optical density of to and with enzyme activity of to u/ml. After 10hrs of incubation in the shaker incubator these parameters tend to decrease (Table-4). Similar reports were observed by Rehena et al., Increase in incubation period resulted in decrease in the production of Beta-glucosidase by B. subtilis. This may be due to the fact that after maximum production of Beta-glucosidase enzyme (maximum incubation time), there was production of other byproducts and a depletion of nutrients. These by-products inhibited the growth of the organisms and hence, enzyme formation (Christakopoulos, 1994). Table 4: Measurement of optimum time for Cells growth and enzyme production Period (hr) Cells growth (O/D) Beta-glucosidase activity u/ml Temperature and ph are the most important factors, which markedly influence enzyme activity. B. sutilis had an enzyme activity of 0.50 mg/ml at 40 C and at optical density of As the temperature increased to 60 C, the enzyme activity increased (1.72 u/ml). This was followed by a sharp decrease in enzyme activity at 650 C. Further increase in temperature was followed by a decrease in enzyme activity. The bacterium showed high level of cell growth and Beta-glucosidase production when grown at ph 7.0 in the presence of sucrose for 10 hours. Above this ph the production was decreased (Table-3). Maximum Beta-glucosidase yield (0.67 u/ml/min) was achieved at ph 7 by B. subtilis although, ph 4-10 supported amylase production (Figure 3). The results suggest that maximum Betaglucosidase activity was observed at neutral ph. But considerable amount of activity ( u/ml/min) was obtained at alkaline ph showing the wide application nature of the amylase enzyme identified. This result is in agreement with Oyeleke and Oduwole (2009) and Daniel et al., (2010). Who stated in their report that most bacterial enzymes function between a ph range of 6 and 8. The results suggest that there is stimulation of enzymes at a neutral ph. Similar observations were made by Olajuyigbe and Ajele (2005) who recorded optimum ph of 8.0 for Bacillus species. The study revealed the potential of agricultural wastes capability to produce Beta-glucosidase by B. subtilis.

8 8 5. Conclusion From the results of this work it has revealed that soil and Bacillus subtilis is a good reserved for the isolation of Cellulase producing microorganism for industrial application, this could be due to abundance of cellulosic materials in it. References Bayer, E. A., Lamed, R., Himmel, M. E., (2007). The potential of cellulases and cellulosomes for cellulosic wastes management. Curr Opin Biotechnol; 18: Bergey s Manual of determinative bacteriology (2000). Actinomycetales. 9 th edition. Chen, W. M., Tseng Z. J., Lee K.S., Chang J. S., (2005). Fermentative hydrogen production with Clostridium butyricum CGS5 isolated from anaerobic sewage sludge. International Journal of Hydrogen Energy; 30: Christakopoulos, P., P.W. Goodenough, D. Kekos, B.J. Macris, M. Cleayssens and M.K. Bhat, Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus. Eur. J. Biochem., 224: Daniel, R. M., Peterson, M. E., Danson, M. J., (2010). The molecular basis of the effect of temperature on enzyme activity. Biochem. J., 425(2): Kapdan, L. K., Kaegi F. (2006). Bio-hydrogen production from wastes materials. Enzyme Microbial Technology; 38: Liu, Y., Yu, P., Song, X., Qu, Y., (2008). Hydrogen production from cellulose by co-culture of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17. International Journal of Hydrogen Energy; 33: Lo, Y. C., Saratale, G. D., Chen, W. M., Bai, M. D., Chang, J.S. (2009) Isolation of celluloseehydrolytic bacteria and applications of the cellulolytic enzymes for cellulosic biohydrogen production. Enzyme Microb Tech., 44: Maki, M., Leung, K. L., Qin, W. (2009). The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass. Int J Biol Sci., 5(5): Miller, G. L., (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem., 31: Olajuyigbe, F. M., Ajele, T. O., (2005). Production dynamics of extracellular protease from Bacillus species African Journal of Biotechnology., 4(8): Oyeleke, S. B., Oduwole A. A., (2009). Production of amylase by bacteria isolated from a Cassava wastes dump site in Minna, Niger State, Nigeria. Afr. J. Microbiol. Res., 3(4):

9 9 Rehena, F., Ventaksubbiah, L., Naud, K., (1989). Preliminary studies on the production of Thermo stable amylase by mesophilic strain of Bacillus licheniformis. Chem. Microbiology Technol. Leberism, 12: Saraswati Bai, M., Ravi kumar, D. J., Mukesh kumar, P. Balashanmugam, M. D., Bala kumaran, P.T. Kalaichelvan., (2012). Cellulase production by Bacillus subtilis isolated from cow dung. Archives of Applied Science Research, 4 (1): Singh, V. K. and A. Kumar., (1998), Production and purification of an intracellular cellulase from Bacillus brevis VS-1. Biochem. Mol. Biol. Int., 45: Peng, L., Chen, Y. (2011) Conversion of paper sludge to ethanol by separate hydrolysis and fermentation (SHF) using Saccharomyces cerevisiae. Biomass Bioenerg, 35: ZhangY. H. P., Himmel M. E., Mielenz J. R., (2006) Outlook for cellulose improvement: Screening and selection strategies. Biotechnol Adv., 24:

Isolation, partial purification and characterization of α-amylase from Bacillus subtilis

Isolation, partial purification and characterization of α-amylase from Bacillus subtilis Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res., 2014, 4 (1):49-54 (http://scholarsresearchlibrary.com/archive.html) ISSN : 2231 3168 CODEN (USA)

More information

Chapter 3 SCREENING AND SELECTION OF STRAIN FOR ALKALINE PROTEASE PRODUCTION BY SUBMERGED FERMENTATION

Chapter 3 SCREENING AND SELECTION OF STRAIN FOR ALKALINE PROTEASE PRODUCTION BY SUBMERGED FERMENTATION Chapter 3 SCREENING AND SELECTION OF STRAIN FOR ALKALINE PROTEASE PRODUCTION BY SUBMERGED FERMENTATION - 42 - 3.1 MATERIAL AND METHODS 3.1.1 Isolation of bacterial strains for alkaline protease production

More information

Isolation and Characterization of Protease Producing Bacteria from Rhizosphere Soil and Optimization of Protease Production Parameters

Isolation and Characterization of Protease Producing Bacteria from Rhizosphere Soil and Optimization of Protease Production Parameters ISSN: 2319-7706 Special Issue-2 (2015) pp. 58-64 http://www.ijcmas.com Original Research Article Isolation and Characterization of Protease Producing Bacteria from Rhizosphere Soil and Optimization of

More information

Molecular characterization and phylogenetic analysis of protease producing Streptomyces sp. isolated from mangrove sediments

Molecular characterization and phylogenetic analysis of protease producing Streptomyces sp. isolated from mangrove sediments Molecular characterization and phylogenetic analysis of protease producing Streptomyces sp. isolated from mangrove sediments M. Parthasarathy and J. Joel Gnanadoss* Department of Plant Biology and Biotechnology,

More information

Research Article PRODUCTION OF A THERMOSTABLE EXTRACELLULAR PROTEASE FROM THERMOPHILIC BACILLUS SPECIES

Research Article PRODUCTION OF A THERMOSTABLE EXTRACELLULAR PROTEASE FROM THERMOPHILIC BACILLUS SPECIES Research Article PRODUCTION OF A THERMOSTABLE EXTRACELLULAR PROTEASE FROM THERMOPHILIC BACILLUS SPECIES S. Suman *1 and K. Ramesh 1 1 Department of Pharmaceutical Biotechnology, Karnataka College of Pharmacy,

More information

Int. J. Pharm. Sci. Rev. Res., 32(2), May June 2015; Article No. 26, Pages:

Int. J. Pharm. Sci. Rev. Res., 32(2), May June 2015; Article No. 26, Pages: Research Article Enhanced Production of Cellulase on Different Fruit Peel Under Submerged Fermentation M. Kannahi*, S. Elangeswari PG and Research Department of Microbiology, Sengamala Thayaar Educational

More information

Isolation and Characterization of Protease Producing Bacteria from Soil Samples of District Kohat, Pakistan

Isolation and Characterization of Protease Producing Bacteria from Soil Samples of District Kohat, Pakistan Journal of Bio-Molecular Sciences (JBMS) (2014) 2(1): 1-5. Isolation and Characterization of Protease Producing Bacteria from Soil Samples of District Kohat, Pakistan Imran Shah 1, Nasir Azam 1, Ghias

More information

Production of bacterial amylase by Bacillus species isolated from rice husk dumpsites in Sokoto metropolis, Nigeria

Production of bacterial amylase by Bacillus species isolated from rice husk dumpsites in Sokoto metropolis, Nigeria Available online at http://ajol.info/index.php/ijbcs Int. J. Biol. Chem. Sci. 5(1): 380-385, February 2011 ISSN 1991-8631 Short Communication http://indexmedicus.afro.who.int Production of bacterial amylase

More information

Isolation and screening of protease producing bacteria from different market soils

Isolation and screening of protease producing bacteria from different market soils International Journal of Research in BioSciences Volume 7 Issue 3, pp. (35-40), June 2018 Available online at http://www.ijrbs.in ISSN 2319-2844 Research Paper Isolation and screening of protease producing

More information

Accepted 9 December, 2011

Accepted 9 December, 2011 Vol. 8(9), pp. 872-877, 26 February, 14 DOI: 1.5897/AJMR11.1273 ISSN 1996-8 14 Academic Journals http://www.academicjournals.org/ajmr African Journal of Microbiology Research Full Length Research Paper

More information

Cellulosic Conversion to Bioethanol from Pongamia Pod A Biodiesel Industry Waste

Cellulosic Conversion to Bioethanol from Pongamia Pod A Biodiesel Industry Waste International OPEN ACCESS Journal Of Modern Engineering Research (IJMER) Cellulosic Conversion to Bioethanol from Pongamia Pod A Biodiesel Industry Waste Yashaswi R.Metri 1, Dr.Bharati S.Meti 2 Department

More information

MOLECULAR IDENTIFICATION OF THE MOST COMMON CELLULASE PRODUCING-BACILLUS FROM FOREST SOILS

MOLECULAR IDENTIFICATION OF THE MOST COMMON CELLULASE PRODUCING-BACILLUS FROM FOREST SOILS : 2250-2256 ISSN: 2277 4998 MOLECULAR IDENTIFICATION OF THE MOST COMMON CELLULASE PRODUCING-BACILLUS FROM FOREST SOILS TOULAMI S 1, JORSHARI S 1, FAZELTOLAMI H 2, PAST S 3, SEYED ALI ROTEH SM 4, ASOUDEHMOGHANLOO

More information

Thermostable 휶 -Amylase Activity from Thermophilic Bacteria Isolated from Bora Hot Spring, Central Sulawesi

Thermostable 휶 -Amylase Activity from Thermophilic Bacteria Isolated from Bora Hot Spring, Central Sulawesi Journal of Physics: Conference Series PAPER OPEN ACCESS Thermostable 휶 -Amylase Activity from Thermophilic Bacteria Isolated from Bora Hot Spring, Central Sulawesi To cite this article: F M Gazali and

More information

International Journal of Pharma and Bio Sciences

International Journal of Pharma and Bio Sciences Research Article Biotechnology International Journal of Pharma and Bio Sciences ISSN 0975-6299 ISOLATION, IDENTIFICATION, PARTIAL PURIFICATION, OPTIMIZATION AND CHARACTERIZATION OF ALKALINE PROTEASE FROM

More information

ISOLATION AND SCREENING OF AMYLASE PRODUCING BACTERIA FROM DIFFERENT MARKET SOILS

ISOLATION AND SCREENING OF AMYLASE PRODUCING BACTERIA FROM DIFFERENT MARKET SOILS Biotechnology Society www.bti.org.in ISSN 974-1453 Research Article ISOLATION AND SCREENING OF AMYLASE PRODUCING BACTERIA FROM DIFFERENT MARKET SOILS M Tamilelakkiya and Radha Palaniswamy* Department of

More information

Production and characterization of biosurfactant from Pseudomonas spp

Production and characterization of biosurfactant from Pseudomonas spp ISSN: 2319-7706 Volume 4 Number 1 (2015) pp. 245-253 http://www.ijcmas.com Original Research Article Production and characterization of biosurfactant from Pseudomonas spp S.K.Arora 2, Jitesh Sony 1, Anayata

More information

BIODEGRADATION OF CRUDE OIL BY GRAVIMETRIC ANALYSIS

BIODEGRADATION OF CRUDE OIL BY GRAVIMETRIC ANALYSIS BIODEGRADATION OF CRUDE OIL BY GRAVIMETRIC ANALYSIS Chithra. S 1, Hema Shenpagam. N 2 1,2 PG and Research Department of Microbiology, Hindusthan College of Arts and Science,Coimbatore, Tamilnadu, (India)

More information

Pretreatment Methods for Banana Peel as a Substrate for the Bioproduction of Ethanol in SHF and SSF

Pretreatment Methods for Banana Peel as a Substrate for the Bioproduction of Ethanol in SHF and SSF Pretreatment Methods for Banana Peel as a Substrate for the Bioproduction of Ethanol in SHF and SSF Nuttiya Chantawongsa Division of Biochemical Technology, School of Bioresources and Technology, King

More information

AN ENZYMATIC PROCESS OF BIOETHANOL PRODUCTION USING AGRICULTURAL WASTES BY Saccharomyces cerevisiae (MTCC 173) AND Zymomonas mobilis (MTCC 2427)

AN ENZYMATIC PROCESS OF BIOETHANOL PRODUCTION USING AGRICULTURAL WASTES BY Saccharomyces cerevisiae (MTCC 173) AND Zymomonas mobilis (MTCC 2427) AN ENZYMATIC PROCESS OF BIOETHANOL PRODUCTION USING AGRICULTURAL WASTES BY Saccharomyces cerevisiae (MTCC 173) AND Zymomonas mobilis (MTCC 2427) A. Pranavya 1, C. Saravanamurugan 1 and S. Rajendran 2.

More information

PROCESS OPTIMIZATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE ENZYME FROM BACILLUS ALTITUDINIS (MCCB 0014)

PROCESS OPTIMIZATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE ENZYME FROM BACILLUS ALTITUDINIS (MCCB 0014) Academic Sciences International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 975-1491 Vol 4, Issue 3, 212 Research Article PROCESS OPTIMIZATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE

More information

Degradation assessment of low density polythene (LDP) and polythene (PP) by an indigenous isolate of Pseudomonas stutzeri

Degradation assessment of low density polythene (LDP) and polythene (PP) by an indigenous isolate of Pseudomonas stutzeri Journal of Scientific & Industrial Research Vol. 63, March 2004, pp 293-296 Degradation assessment of low density polythene (LDP) and polythene (PP) by an indigenous isolate of Pseudomonas stutzeri Anjana

More information

Isolation of Cellulolytic Fungi and their Degradation on Cellulosic Agricultural Wastes

Isolation of Cellulolytic Fungi and their Degradation on Cellulosic Agricultural Wastes Volume 2, Issue 8 January 214 458 RESEARCH ARTICLE ISSN: 2278-5213 Isolation of Cellulolytic Fungi and their Degradation on Cellulosic Agricultural Wastes Sivakumaran Sivaramanan Post Graduate Institute

More information

Bacterial strain and growth condition

Bacterial strain and growth condition International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.1, No.2, pp 344-348, April-June 2009 Microbial Production of Hydrogen from Sugarcane Bagasse using Bacillus Sp. T.R.Manikkandan

More information

Production of Extracellular Protease and Determination of Optimise Condition by Bacillus Licheniformis BBRC

Production of Extracellular Protease and Determination of Optimise Condition by Bacillus Licheniformis BBRC CHEMICAL ENGINEERING TRANSACTIONS Volume 21, 2010 Editor J. J. Klemeš, H. L. Lam, P. S. Varbanov Copyright 2010, AIDIC Servizi S.r.l., ISBN 978-88-95608-05-1 ISSN 1974-9791 DOI: 10.3303/CET1021242 1447

More information

SELECTED QUESTIONS F ROM OLD MICRO 102 QUIZZES PART I EXPERIMENTS 1 THROUGH 7

SELECTED QUESTIONS F ROM OLD MICRO 102 QUIZZES PART I EXPERIMENTS 1 THROUGH 7 SELECTED QUESTIONS F ROM OLD MICRO 102 QUIZZES PART I EXPERIMENTS 1 THROUGH 7 Question numbers refer to the applicable experiment. Questions with blanks are multiple true-false questions unless otherwise

More information

IMPACT OF MEDIA ON ISOLATION OF DEXTRANASE PRODUCING FUNGAL STRAINS

IMPACT OF MEDIA ON ISOLATION OF DEXTRANASE PRODUCING FUNGAL STRAINS Journal of Scientific Research Vol. 55, 2 : 7-76 Banaras Hindu University, Varanasi ISSN : 447-9483 IMPACT OF MEDIA ON ISOLATION OF DEXTRANASE PRODUCING FUNGAL STRAINS Priyanka Jaiswal and Santosh Kumar

More information

Characterization of the Lactobacillus isolated from different curd samples

Characterization of the Lactobacillus isolated from different curd samples African Journal of Biotechnology Vol. 11(79), pp. 14448-14452, 2 October, 2012 Available online at http://www.academicjournals.org/ajb DOI: 10.5897/AJB11.310 ISSN 1684 5315 2012 Academic Journals Full

More information

ALKALINE PROTEASE PRODUCTION BY THERMOPHILIC AND ALKALOPHILIC HALOTOLERANT BACILLUS SP. STRAIN TD : A PROMISING ENZYME PRODUCER FOR BIOTECHNOLOGICAL APPLICATION Dhara Desai $ and Trupti K Vyas* *Food Quality

More information

INVESTIGATION ON CONVERSION OF FLOWER WASTES INTO BIOETHANOL AND PERFORMANCE EVALUATION ON SINGLE CYLINDER IC ENGINE

INVESTIGATION ON CONVERSION OF FLOWER WASTES INTO BIOETHANOL AND PERFORMANCE EVALUATION ON SINGLE CYLINDER IC ENGINE INVESTIGATION ON CONVERSION OF FLOWER WASTES INTO BIOETHANOL AND PERFORMANCE EVALUATION ON SINGLE CYLINDER IC ENGINE COLLEGE : BAPUJI INSTITUTE OF ENGINEERING AND TECHNOLOGY, DAVANGERE DEPARTMENT : MECHANICAL

More information

Application of green fluorescent protein signal for effective monitoring of fermentation processes

Application of green fluorescent protein signal for effective monitoring of fermentation processes Available online atwwwscholarsresearchlibrarycom Archives of Applied Science Research, 214, 6 (6):17-111 (http://scholarsresearchlibrarycom/archivehtml) ISSN 975-58X CODEN (USA) AASRC9 Application of green

More information

Production and purification of enzyme Xylanase by Aspergillus niger

Production and purification of enzyme Xylanase by Aspergillus niger ISSN: 2319-7706 Volume 3 Number 10 (2014) pp. 664-668 http://www.ijcmas.com Original Research Article Production and purification of enzyme Xylanase by Aspergillus niger S.Subbulakshmi and Priya R Iyer*

More information

Production and characterization of cellulolytic enzymes by Pleurotus florida

Production and characterization of cellulolytic enzymes by Pleurotus florida Production and characterization of cellulolytic enzymes by Pleurotus florida Mycosphere Goyal M * and Soni G Department of Biochemistry, Punjab Agricultural University, Ludhiana-141004. Punjab, India Goyal

More information

Effect of Toluene on Fungal Growth and Amylase Production-A Step towards Exploration of Enzymes for Industrial Applications

Effect of Toluene on Fungal Growth and Amylase Production-A Step towards Exploration of Enzymes for Industrial Applications International Review of Applied Engineering Research. ISSN 2248-9967 Volume 4, Number 2 (2014), pp. 117-122 Research India Publications http://www.ripublication.com/iraer.htm Effect of Toluene on Fungal

More information

Amylase activity of a starch degrading bacteria isolated from soil receiving kitchen wastes

Amylase activity of a starch degrading bacteria isolated from soil receiving kitchen wastes African Journal of Biotechnology Vol. 7 (18), pp. 3326-3331, 17 September, 2008 Available online at http://www.academicjournals.org/ajb ISSN 1684 5315 2008 Academic Journals Full Length Research Paper

More information

Production of Protease and Growth Characteristics of Aspergillus sydowii. Corresponding Author

Production of Protease and Growth Characteristics of Aspergillus sydowii. Corresponding Author Nature and Science, 11;9(5) Production of Protease and Growth Characteristics of Aspergillus sydowii 1 Arun Kumar Sharma, Vinay Sharma and 3 Jyoti Saxena 1 & Department of Bioscience and Biotechnology,

More information

Optimization of Protease Enzyme Production Using Bacillus Sp. Isolated from Different Wastes

Optimization of Protease Enzyme Production Using Bacillus Sp. Isolated from Different Wastes Botany Research International 2 (2): 83-87, 2009 ISSN 1995-8951 IDOSI Publications, 2009 Optimization of Protease Enzyme Production Using Bacillus Sp. Isolated from Different Wastes 1 1 Udandi Boominadhan,

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(6):872-876 Effect of critical medium components on antimicrobial

More information

Reduction of Ferric Compounds by Soil Bacteria

Reduction of Ferric Compounds by Soil Bacteria No. 3, Volume 10 of the Jouml of General Microbiology was issued on 10 June 1954. BROMFIELD, S. M. (1954). J. gen. Microbiol. 11, 14. Reduction of Ferric Compounds by Soil Bacteria BY S. M. BROMFIELD Microbiology

More information

MATERIALS & METHODS Microorganisms and cultivation. Keywords: Corncob; Cellulosic hydrolysates; Streptomyces sp.; Reducing sugar; Bioethanol

MATERIALS & METHODS Microorganisms and cultivation. Keywords: Corncob; Cellulosic hydrolysates; Streptomyces sp.; Reducing sugar; Bioethanol Pretreatment and hydrolysis of cellulosic agricultural wastes with a cellulaseproducing Streptomyces for bioethanol production Chuan-Liang Hsu a, Ku-Shang Chang b, Yi-Huang Chang b, Hung-Der Jang b, *

More information

Isolation and identification of cellulose degrading bacteria and optimization of the cellulase production

Isolation and identification of cellulose degrading bacteria and optimization of the cellulase production International Journal of Research in Biosciences Vol. 5 Issue 3, pp. (58-67), July 2016 Available online at http://www.ijrbs.in ISSN 2319-2844 Research Paper Abstract Isolation and identification of cellulose

More information

Applying the mutation of Bacillus subtilis and the optimization of feather fermentation medium to improve Keratinase activity

Applying the mutation of Bacillus subtilis and the optimization of feather fermentation medium to improve Keratinase activity Advances in Biological Chemistry, 2012, 2, 64-69 http://dx.doi.org/10.4236/abc.2012.21008 Published Online February 2012 (http://www.scirp.org/journal/abc/) ABC Applying the mutation of Bacillus subtilis

More information

Isolation and characterization of a solvent tolerant alkaliphilic marine bacteria

Isolation and characterization of a solvent tolerant alkaliphilic marine bacteria International Journal of Scientific & Engineering Research Volume 3, Issue 8, August-2012 1 Isolation and characterization of a solvent tolerant alkaliphilic marine bacteria Roselin Alex*, and Sarita G.

More information

Pelagia Research Library. Feather Waste biodegradation as a source of Amino acids

Pelagia Research Library. Feather Waste biodegradation as a source of Amino acids Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2011, 1 (2):56-63 ISSN: 2248 9215 Feather Waste biodegradation as a source of Amino acids Avinash Srivastava,

More information

Optimization of Cellulolytic Bacteria from Cellulose Waste Materials and its Activity

Optimization of Cellulolytic Bacteria from Cellulose Waste Materials and its Activity Research Article Optimization of Cellulolytic Bacteria from Cellulose Waste Materials and its Activity T. Vinotha, N. Uma Maheswari* PG and Research Department of Microbiology, Sengamala Thaayar Educational

More information

Growth kinetic and modeling of ethanol production by wilds and mutant Saccharomyces cerevisae MTCC 170

Growth kinetic and modeling of ethanol production by wilds and mutant Saccharomyces cerevisae MTCC 170 Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2015, 5(4):1-6 ISSN: 2248 9215 CODEN (USA): EJEBAU Growth kinetic and modeling of ethanol production by wilds

More information

Production Of Protease By Submerged Fermentation Using Rhizopus Microsporus Var Oligospous

Production Of Protease By Submerged Fermentation Using Rhizopus Microsporus Var Oligospous ISPUB.COM The Internet Journal of Microbiology Volume 9 Number 1 Production Of Protease By Submerged Fermentation Using Rhizopus Microsporus Var Oligospous L Sarao, M Arora, V Sehgal, S Bhatia Citation

More information

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB 4789.

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB 4789. Translated English of Chinese Standard: Translated by: www.chinesestandard.net Email: Wayne@ChineseStandard.net National Standard of the People s Republic of China GB National food safety standard Food

More information

ALKALINE PROTEASE PRODUCTION BY PSEUDOMONAS AERUGINOSA ISOLATED FROM THE GUT OF PILA OVATA

ALKALINE PROTEASE PRODUCTION BY PSEUDOMONAS AERUGINOSA ISOLATED FROM THE GUT OF PILA OVATA Journal of Global Biosciences Vol. 2(5), 213, pp. 126-131 http://mutagens.co.in ALKALINE PROTEASE PRODUCTION BY PSEUDOMONAS AERUGINOSA ISOLATED FROM THE GUT OF PILA OVATA Ariole, C.N. and E. Ilega Department

More information

AMYLASE SYNTHESIS IN ELEVATED LEVEL BY OPTIMIZING FERMENTATION PARAMETERS FROM ASPERGILLUS VERSICOLOR

AMYLASE SYNTHESIS IN ELEVATED LEVEL BY OPTIMIZING FERMENTATION PARAMETERS FROM ASPERGILLUS VERSICOLOR ISSN:2249-1236 INTERNATIONAL JOURNAL OF RESEARCH AND REVIEWS IN PHARMACY AND APPLIED SCIENCES Research Article AMYLASE SYNTHESIS IN ELEVATED LEVEL BY OPTIMIZING FERMENTATION PARAMETERS FROM ASPERGILLUS

More information

Effects of Micronutrients on the Production of Heparinase from Bacillus lentus

Effects of Micronutrients on the Production of Heparinase from Bacillus lentus Effects of Micronutrients on the Production of Heparinase from Bacillus lentus Dhanalakshmi.R 1, Manasa.A 2, Ramesh Babu.N.G 3 1, 2 Department of Biotechnology, Adhiyamaan College of Engineering, Hosur,

More information

Volume: 2: Issue-3: July-Sept ISSN EFFECT OF NITROGEN SOURCES ON MICROBIAL PRODUCTION OF XYLITOL. K. Srivani 1 and Y.

Volume: 2: Issue-3: July-Sept ISSN EFFECT OF NITROGEN SOURCES ON MICROBIAL PRODUCTION OF XYLITOL. K. Srivani 1 and Y. Volume: 2: Issue-3: July-Sept -2011 ISSN 0976-4550 EFFECT OF NITROGEN SOURCES ON MICROBIAL PRODUCTION OF XYLITOL K. Srivani 1 and Y. Pydi Setty 2 1 Department of Chemical Engineering, National Institute

More information

GUIDELINES FOR WRITING A LAB REPORT FOR BIOL 215L (MICROBIOLOGY FOR HEALTHCARE PROFESSIONALS)

GUIDELINES FOR WRITING A LAB REPORT FOR BIOL 215L (MICROBIOLOGY FOR HEALTHCARE PROFESSIONALS) GUIDELINES FOR WRITING A LAB REPORT FOR BIOL 215L (MICROBIOLOGY FOR HEALTHCARE PROFESSIONALS) Your lab report will focus only on your unknown bacteria, which you collected, cultured, isolated, analyzed,

More information

Some Industrially Important Microbes and Their Products

Some Industrially Important Microbes and Their Products 2 Some Industrially Important Microbes and Their Products 2.1. ENZYME PRODUCING MICROBES Type of enzyme Substrate Microorganism Amylase Starch Saccharomyces diastaticus Protease Proteins Bacillus sp. Lipase

More information

Comparison of Acidic and Basic Pretreatment on Saccharification of Water Hyacinth

Comparison of Acidic and Basic Pretreatment on Saccharification of Water Hyacinth Comparison of Acidic and Basic Pretreatment on Saccharification of Water Hyacinth Sathvik Varma V 1, Hari Narayanan T R 2, Kamala K 3, Shivashankar B 4, Jagadish H Patil 5 Corresponding author, UG Chemical

More information

SECONDARY COLONY FORMATION BY BACILLUS SUBTILIS ON EOSINE

SECONDARY COLONY FORMATION BY BACILLUS SUBTILIS ON EOSINE SECONDARY COLONY FORMATION BY BACILLUS SUBTILIS ON EOSINE METHYLENE BLUE AGAR K. K. SHAH' AND V. N. IYER2 Microbiology Department, S. B. Garda College, Navsari, India Received for publication November

More information

Results and Discussion

Results and Discussion 4.0. RESULTS AND DISCUSSION: The results obtained during the course of present study are presented and discussed in this chapter. This part of the thesis elucidates isolation of microbes from different

More information

INTRODUCTION water-soluble Figure 1.

INTRODUCTION water-soluble Figure 1. INTRODUCTION Natural waters contain bacteria. The aerobic gram negative bacillus of the genera Psedomonas, Alcalignes, and Flavobacterium are common in natural waters. Many of these bacteria are able to

More information

Solid-state fermentation of waste cabbage by Penicillium notatum NCIM NO-923 for production and characterization of cellulases

Solid-state fermentation of waste cabbage by Penicillium notatum NCIM NO-923 for production and characterization of cellulases Journal of Scientific & Industrial Research 714 Vol. 68, August 29, pp. 714-718 J SCI IND RES VOL 68 AUGUST 29 Solid-state fermentation of waste cabbage by Penicillium notatum NCIM NO-923 for production

More information

Isolation of Salmonella from Natural Sources Representing High Potential for Biofilm Formations

Isolation of Salmonella from Natural Sources Representing High Potential for Biofilm Formations Isolation of Salmonella from Natural Sources Representing High Potential for Biofilm Formations Manutsawee Tammakritsada and Tatsaporn Todhanakasem Faculty of Biotechnology, Assumption University, Bangkok,

More information

BIOACCUMULATION OF LEAD METAL IONS FROM PAINT INDUSTRY EFFLUENTS BY INDIGENOUS BACTERIA

BIOACCUMULATION OF LEAD METAL IONS FROM PAINT INDUSTRY EFFLUENTS BY INDIGENOUS BACTERIA BIOACCUMULATION OF LEAD METAL IONS FROM PAINT INDUSTRY EFFLUENTS BY INDIGENOUS BACTERIA PROJECT REFERENCE NO. : 37S0091 COLLEGE : SAPTHAGIRI COLLEGE OF ENGINEERING,BANGALORE BRANCH : BIOTECHNOLOGY GUIDE

More information

Screening of α-amylase producing bacteria from tannery wastes of Hazaribag, Bangladesh

Screening of α-amylase producing bacteria from tannery wastes of Hazaribag, Bangladesh Jahangirnagar University J. Biol. Sci. 5(2): 1-10, 2016 (December) Screening of α-amylase producing bacteria from tannery wastes of Hazaribag, Bangladesh Md. Rayhan Islam, Omit Kumer Mondol 1, Md. Saimoon

More information

Parametric Optimization of Media for the Crude Oil Degrading Bacteria Isolated from Crude Oil Contaminated Site

Parametric Optimization of Media for the Crude Oil Degrading Bacteria Isolated from Crude Oil Contaminated Site ISSN: 2319-7706 Volume 4 Number 2 (2015) pp. 322-328 http://www.ijcmas.com Original Research Article Parametric Optimization of Media for the Crude Oil Degrading Bacteria Isolated from Crude Oil Contaminated

More information

Isolation, Characterization, Identification and Screening of Amylase Producing Bacteria from Soil under System of Rice Intensification (SRI) Technique

Isolation, Characterization, Identification and Screening of Amylase Producing Bacteria from Soil under System of Rice Intensification (SRI) Technique Available online at www.ijpab.com Shruti and Banik Int. J. Pure App. Biosci. 4 (5): 153-159 (2016) ISSN: 2320 7051 DOI: http://dx.doi.org/10.18782/2320-7051.2372 ISSN: 2320 7051 Int. J. Pure App. Biosci.

More information

Alternative Feed-stocks for Bioconversion to Ethanol: a techno-commercial appraisal

Alternative Feed-stocks for Bioconversion to Ethanol: a techno-commercial appraisal Alternative Feed-stocks for Bioconversion to Ethanol: a techno-commercial appraisal Subhash Chand Formerly, Professor & Head: Department of Biochemical Engineering & Biotechnology Indian Institute of Technology

More information

Efficiency of Waste Banana Peels in Bio-ethanol Production

Efficiency of Waste Banana Peels in Bio-ethanol Production www.stmjournals.com Efficiency of Waste Banana Peels in Bio-ethanol Production Apurva Barve 1, *, Kishori Tarfe 2 1 Department of Environmental Science, Institute of Science, Mumbai, Maharashtra, India

More information

Microbial production of protease by Bacillus Cereus using cassava waste water

Microbial production of protease by Bacillus Cereus using cassava waste water Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2014, 4(2):19-24 ISSN: 2248 9215 CODEN (USA): EJEBAU Microbial production of protease by Bacillus Cereus using

More information

Isolation and screening of bacteria associated with fermented cassava peels for linamarase production

Isolation and screening of bacteria associated with fermented cassava peels for linamarase production IJAMBR 5 (2017) 20-26 ISSN 2053-1818 Isolation and screening of bacteria associated with fermented cassava peels for linamarase Adeleke B. S.*, Olaniyi O. O. and Akinyele B. J. Department of Microbiology,

More information

Production and Optimal Performance Studies of Glucose Isomerase from Agricultural Raw Materials

Production and Optimal Performance Studies of Glucose Isomerase from Agricultural Raw Materials Global Advanced Research Journal of Microbiology (ISSN: 2315-5116) Vol. 1(7) pp. 108-119, August, 2012 Available online http://garj.org/garjm/index.htm Copyright 2012 Global Advanced Research Journals

More information

Production and Optimization of α-amylase by Bacillus licheniformis ATCC 6346 in Lab Bench-Scale Fermenter

Production and Optimization of α-amylase by Bacillus licheniformis ATCC 6346 in Lab Bench-Scale Fermenter Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res.,, ():9- (http://scholarsresearchlibrary.com/archive.html) ISSN : 3 368 CODEN (USA) : JMBRB4 Production

More information

The optimization of fermentation conditions particularly physical and chemical

The optimization of fermentation conditions particularly physical and chemical 4.1 Preamble The optimization of fermentation conditions particularly physical and chemical parameters are of primary importance in the development of any fermentation process owing to their impact on

More information

Enzymatic synthesis of levan polysaccharide by Bacillus licheniformis levansucrase. Imen Dahech*, Rania Bredai, Karima Srih

Enzymatic synthesis of levan polysaccharide by Bacillus licheniformis levansucrase. Imen Dahech*, Rania Bredai, Karima Srih ISSN : 0974-7427 Volume 8 Issue 5 Enzymatic synthesis of levan polysaccharide by Bacillus licheniformis levansucrase Imen Dahech*, Rania Bredai, Karima Srih Biochemistry laboratory, Faculty of sciences

More information

STUDIES ON THE CELL WALL LYTIC ENZYMES PRODUCED BY STREPTOMYCES SPECIES PART 1. THE STRAINS AND THEIR LYTIC ACTIVITY TOWARD SACCHAROMYCES

STUDIES ON THE CELL WALL LYTIC ENZYMES PRODUCED BY STREPTOMYCES SPECIES PART 1. THE STRAINS AND THEIR LYTIC ACTIVITY TOWARD SACCHAROMYCES J. Gen. Appl. Microbiol. Vol. 6, No. 1, 1960 STUDIES ON THE CELL WALL LYTIC ENZYMES PRODUCED BY STREPTOMYCES SPECIES PART 1. THE STRAINS AND THEIR LYTIC ACTIVITY TOWARD SACCHAROMYCES AKIRA FURUYA and YONOSUKE

More information

Production, Isolation and Purification of Peroxidase Using Bacillus Subtilis

Production, Isolation and Purification of Peroxidase Using Bacillus Subtilis 214 1 st International Congress on Environmental, Biotechnology, and Chemistry Engineering IPCBEE vol.64 (214) (214) IACSIT Press, Singapore DOI: 1.7763/IPCBEE. 214. V64. 5 Production, Isolation and Purification

More information

STUDIES ON ISOLATION, CHARACTERIZATION AND GROWTH OF CHLORPYRIFOS DEGRADING BACTERIA FROM FARM SOIL

STUDIES ON ISOLATION, CHARACTERIZATION AND GROWTH OF CHLORPYRIFOS DEGRADING BACTERIA FROM FARM SOIL 220 STUDIES ON ISOLATION, CHARACTERIZATION AND GROWTH OF CHLORPYRIFOS DEGRADING BACTERIA FROM FARM SOIL 1. Diksha Sateesh Bakre Research scholar Dept. of Biotechnology and Microbiology Karnatak University

More information

Production Of Cellulase From Corn Cobs By Aspergillus niger Under Submerged Fermentation

Production Of Cellulase From Corn Cobs By Aspergillus niger Under Submerged Fermentation International Journal of ChemTech Research CODEN (USA): IJCRGG ISSN : 0974-4290 Vol.6, No.5, pp 2900-2904, Aug-Sept 2014 Production Of Cellulase From Corn Cobs By Aspergillus niger Under Submerged Fermentation

More information

Optimization of Solid State Fermentation Conditions for the Production of Cellulase by Using Trichoderma Reesei

Optimization of Solid State Fermentation Conditions for the Production of Cellulase by Using Trichoderma Reesei Available online at www.scholarsresearchlibrary.com European Journal of Applied Engineering and Scientific Research, 212, 1 (4):196-2 (http://scholarsresearchlibrary.com/archive.html) ISSN: 2278 41 Optimization

More information

Final text for addition to The International Pharmacopoeia

Final text for addition to The International Pharmacopoeia March 2012 3.3.2 MICROBIOLOGICAL EXAMINATION OF NON-STERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS Final text for addition to The International Pharmacopoeia This monograph was adopted at the Forty-sixth

More information

á62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS

á62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS USP 40 Microbiological Tests / á62ñ Microbiological Examination 1 á62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS INTRODUCTION The tests described hereafter

More information

Volume: I: Issue-2: Aug-Oct ISSN SCREENING AND CHARACTERIZATION OF PROTEASE FROM BACILLUS SP

Volume: I: Issue-2: Aug-Oct ISSN SCREENING AND CHARACTERIZATION OF PROTEASE FROM BACILLUS SP Volume: I: Issue-2: Aug-Oct -2010 ISSN 0976-4550 SCREENING AND CHARACTERIZATION OF PROTEASE FROM BACILLUS SP Siddalingeshwara K.G 1, Uday.J 2, Huchesh C.H 2, Puttaraju H.P 2, Karthic J 3, Sudipta K.M 1,

More information

Effects of ph on Inoculum Production of Aspergillus tubingensis on the Acid Rice Media

Effects of ph on Inoculum Production of Aspergillus tubingensis on the Acid Rice Media Effects of ph on Inoculum Production of Aspergillus tubingensis on the Acid Rice Media Bambang Irawan 1,*, Dea Putri Andeska 1, CN Ekowati 1, Yulianty 1 and Sutopo Hadi 2,# 1 Department of Biology, Faculty

More information

Lab Date Experiment Reports, Midterms, Reminders

Lab Date Experiment Reports, Midterms, Reminders Lab Date Experiment Reports, Midterms, Reminders Lab 1 Lab 2 Jan 5-6 Jan 7-8 o Registration, introductory remarks, safety lecture, etc. Sterile technique. o Expt 1: Microbes in the environment o Expt 2:

More information

Improvements in Bioethanol Production Process from Straw

Improvements in Bioethanol Production Process from Straw Improvements in Bioethanol Production Process from Straw Heike Kahr,*, Alexander G. Jäger Upper Austria University of Applied Sciences Research and Development Ltd, Campus Wels Stelzhamerstrasse, A- Wels,

More information

Ameer Khusro* Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai, Tamil Nadu, India. Abstract. 2.

Ameer Khusro* Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai, Tamil Nadu, India. Abstract. 2. Statistical Approach for Optimization of Independent Variables on Alkali-Thermo Stable Protease Production from Bacillus Licheniformis Strain BIHPUR 0104 Ameer Khusro* Department of Plant Biology and Biotechnology,

More information

Supporting Information. Cationic Conjugated Polymers-Induced Quorum Sensing of Bacteria Cells

Supporting Information. Cationic Conjugated Polymers-Induced Quorum Sensing of Bacteria Cells Supporting Information Cationic Conjugated Polymers-Induced Quorum Sensing of Bacteria Cells Pengbo Zhang, Huan Lu, Hui Chen, Jiangyan Zhang, Libing Liu*, Fengting Lv, and Shu Wang* Beijing National Laboratory

More information

INVITRO COMPATIBILITY EVALUATION FOR THE BIOCONVERSION OF DOMESTIC SOLID WASTES BY MIXED CULTURES OF MICRO-ORGANISMS

INVITRO COMPATIBILITY EVALUATION FOR THE BIOCONVERSION OF DOMESTIC SOLID WASTES BY MIXED CULTURES OF MICRO-ORGANISMS INVITRO COMPATIBILITY EVALUATION FOR THE BIOCONVERSION OF DOMESTIC SOLID WASTES BY MIXED CULTURES OF MICRO-ORGANISMS Ashwitha Kodaparthi 1, Pavan Kumar Pindi 2, A. Swaroopa Rani 3 1 Department of Microbiology,

More information

Production of cellulase by submerged and solid-state cultures and yeasts selection for conversion of lignocellulose to ethanol

Production of cellulase by submerged and solid-state cultures and yeasts selection for conversion of lignocellulose to ethanol Romanian Biotechnological Letters Vol. 14, No. 2, 2009, pp. 4275-4281 Copyright 2009 Bucharest University Printed in Romania. All rights reserved Romanian Society of Biological Sciences ORIGINAL PAPER

More information

GB Translated English of Chinese Standard: GB

GB Translated English of Chinese Standard: GB Translated English of Chinese Standard: GB4789.35-2016 www.chinesestandard.net Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.35-2016 National food safety standard

More information

Enrichment, Isolation and Identification of Hydrocarbon Degrading Bacteria

Enrichment, Isolation and Identification of Hydrocarbon Degrading Bacteria International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 6 (2015) pp. 708-713 http://www.ijcmas.com Original Research Article Enrichment, Isolation and Identification

More information

CHAPTER III SCREENING, ISOLATION AND DETERMINATION OF ANTIMICROBIAL SPECTRA OF ACTINOMYCETES

CHAPTER III SCREENING, ISOLATION AND DETERMINATION OF ANTIMICROBIAL SPECTRA OF ACTINOMYCETES CHAPTER III SCREENING, ISOLATION AND DETERMINATION OF ANTIMICROBIAL SPECTRA OF ACTINOMYCETES (a) Collection of Samples: A total of nine samples were collected in sterile containers for the systematic screening

More information

Research Article. Production of extracellular protease using bacillus species from the red soil and optimization of protease activity

Research Article. Production of extracellular protease using bacillus species from the red soil and optimization of protease activity Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 5, 7(8):446-453 Research Article ISSN : 975-7384 CODEN(USA) : JCPRC5 Production of extracellular protease using bacillus

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(4):498-503 Optimization of culture conditions for Keratinase

More information

The effect of acid pretreatment on bio-ethanol and bio-hydrogen production from sunflower straw

The effect of acid pretreatment on bio-ethanol and bio-hydrogen production from sunflower straw nd International Conference on Sustainable Solid Waste Management The effect of acid pretreatment on bio-ethanol and bio-hydrogen production from sunflower straw G. Antonopoulou 1, G. Dimitrellos 1, D.

More information

Study on Activity and Stability of Proteases from Bacillus Sp. Produced by Submerged Fermentation

Study on Activity and Stability of Proteases from Bacillus Sp. Produced by Submerged Fermentation 2283 Int. J. Adv. Biol. Biom. Res, 2014; 2 (7), 2283-2287 IJABBR- 2014- eissn: 2322-4827 International Journal of Advanced Biological and Biomedical Research Journal homepage: www.ijabbr.com Original Article

More information

Activity 5.1.5: Student Resource Sheet

Activity 5.1.5: Student Resource Sheet Activity 5.1.5: Student Resource Sheet Biochemical tests are the most definitive way to identify bacterial species. Each biochemical test helps determine a property or characteristic specific to a certain

More information

beta-glucosidase Assay Kit

beta-glucosidase Assay Kit beta-glucosidase Assay Kit Catalog Number KA1611 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...

More information

Influence of media components and ph on the production of alpha amylase from Bacillus sp. SJC B03

Influence of media components and ph on the production of alpha amylase from Bacillus sp. SJC B03 Indian Journal of Applied Microbiology ISSN (Online): 2454 289X, ISSN (Print): 2249 8400 Copyright 2017 IJAM, Chennai, India Volume 20 Number 1 January - June 2017, pp. 55-62 Influence of media components

More information

Characterization of an Alginolytic Marine Bacterium from Decaying Rishiri-kombu Laminaria japonica var. ochotensis

Characterization of an Alginolytic Marine Bacterium from Decaying Rishiri-kombu Laminaria japonica var. ochotensis Nippon Suisan Gakkaishi 58(1), 141-145 (1992) Characterization of an Alginolytic Marine Bacterium from Decaying Rishiri-kombu Laminaria japonica var. ochotensis Tomoo Sawabe,* Yoshio Ezura,* and Takahisa

More information

BACTERIAL BIOFILMS FORMATION AT AIR LIQUID INTERFACES

BACTERIAL BIOFILMS FORMATION AT AIR LIQUID INTERFACES Innovative Romanian Food Biotechnology Vol. 5, Issue of December, 009 009 by Dunărea de Jos University Galaţi Received September 1, 009/ Accepted November 8, 009 RESEARCH ARTICLE BACTERIAL BIOFILMS FORMATION

More information

Chapter 6: Microbial Growth

Chapter 6: Microbial Growth Chapter 6: Microbial Growth 1. Requirements for Growth 2. Culturing Microorganisms 3. Patterns of Microbial Growth 1. Requirements for Growth Factors that affect Microbial Growth Microbial growth depends

More information

Pathogenic Bacteria. culture media. Components of the Typical Culture Medium: Culture Media Importance:

Pathogenic Bacteria. culture media. Components of the Typical Culture Medium: Culture Media Importance: Level4 Lab2: Pathogenic Bacteria culture media Microorganisms, like all other living organisms, require basic nutrients for sustaining their life. All microorganisms have the same basic requirements but

More information