RNA Purification Kits

Transcription

1 GMbiolab Co., Ltd. RNA Purification Kits column format and easy to use High yield and high purity Product Info Speedy operation procedure Product Cat. No. Sample RNA Recovery Format Time Total RNA Purification Kit TR01 Animal cells and tissue, bacteria 10 ~100 µg 1 hr Plant Total RNA Purification Kit TR02 Herb and woody plants, fungi 10 ~100 µg 1 hr GM RNAprep pure Blood Purification GDP433 Whole blood Varies depending on sample type 30~40 min GMamp Virus RNA GDP315-R Whole blood, plasma, serum and other cellfree materials Varies depending on sample type 1 hr GMamp Virus DNA/RNA GDP315 Whole blood, plasma, serum and other cellfree materials Varies depending on sample type 1 hr GM Magnetic Viral DNA/RNA GDP438 Serum, plasma and body fluid Varies depending on sample type Magnetic particles 45 min GMprep DNA/RNA Isolation GDP422 Animal cells and tissue Varies depending on sample type 40 min GMprep Mini pure RNA GDP420 Small sized samples such as microdissected cryosections and tissue ~45 µg 40 min mircute micro RNA Isolation GDP 501 Cells, tissues and blood Varies depending on sample type 1 hr Product Cat. No. Sample Trisolution plus TS-100 Cells, tissues, bacteria, plant, yeast and virus GRNzol Reagent GDP405 Cells and tissues GRNzol-A+ Reagent GDP421 Cells and tissues GeneMark 30

2 GeneMark GMbiolab Co., Ltd. Yield: 10~100 μg ultra pure total RNA depending on the sample type and sample size. Purity: High-quality total RNA is purified using spin column format. RNasefree DNase I is included in the kit to digest contaminating gdna in RNA preparation. Total RNA Purification Kit The Total RNA Purification Kit provides specific buffer system and highcapacity binding glass fiber matrix column to purify high purity RNA from bacteria, animal/plant tissues and cells. Northern blotting, qrt-pcr, poly(a) RNA selection, and RNase protection assays. Figure 1. Non-denaturing gel electrophoresis analysis of total RNA purified from various samples using GeneMark Total RNA Purification Kit and related kit from supplier Q. The RNA purified using related kit from supplier Q contains large amount of genomic DNA contamination. RNA can be obtained within an hour. No phenol / chloroform extraction or alcohol/ isopropanol precipitation. Figure 2. Gel electrophoresis analysis of RT-PCR results of RNA purified using GeneMark Total RNA Purification and related kit from supplier Q. Total RNA was purified from zebrafish and zebrafish EF-1α gene was amplified. Genomic DNA contamination is observed in RNA extracted using related kit from supplier Q by Taq only control. Animal tissue, cell culture, bacteria Lyse (Add 100% EtoH) Brand GeneMark A260/A Total Yield (μg) (average) 378 RNA Wash Solution I Q DNase I treatment Figure 3. Total RNA of E.coli DH5α purified using GM Total RNA Purification Kit and purification kit from supplier Q. Total RNA of E.coli DH5α was purified from 1 ml culture and eluted in 50 μl RNase free H 2 O. 2.5 μl of RNA were loaded onto each lane of agarose gel. GeneMark: TR01 Q: supplier Q RNA Wash Solution I RNA Wash Solution II (2 times) Elute Ultrapure total RNA DESCRIPTION Cat.No. REACTION Total RNA Purification Kit TR01 50 Total RNA Purification Kit TR GeneMark

3 Yield: 10~100 μg ultra pure total RNA depending on the sample type and sample size. Purity: High-quality total RNA is purified using glass fiber matrix column. Plant contaminants such as polyphenolics and polysaccharides can be removed by the kit; DNase I is also included to digest contaminating DNA during RNA preparation. RNA can be obtained within an hour. No phenol/chloroform extraction or alcohol/ isopropanol precipitation. Plant Total RNA Purification Kit Plant Total RNA Purification Kit is designed for herbs, woody plants, succulent plants, and fungi. This kit provides two alternative lysis system, Lysis solution A (guanidine thiocyanate) and Lysis solution B (SDS/anti-oxifants). Choose the most suitable lysis system combining with high-capacity binding glass fiber matrix column to purify the total RNA from a wide variety of plant and fungal samples. Northern blotting, qrt-pcr, poly(a) RNA selection, and RNase protection assays. Figure 1. Non-denaturing gel electrophoresis analysis of the total RNA purified from various plant leaf samples using GeneMark Plant Total RNA Purification Kit with Lysis Solution B system, and related kit from supplier Q. The rice RNA purified using related kit from supplier Q contains large amount of genomic DNA contamination. Plant tissue Lyse RT-PCR 0.9 kb gdna PCR kb filter Add 100% EtoH or binding buffer Figure 2. Gel electrophoresis analysis of RT-PCR of rice actin gene. Total RNA was purified using GeneMark Plant Total RNA Purification Kit and related kit from supplier Q. Genomic DNA contamination was observed from RNA purified using kit from supplier Q by both RT and Taq only control. RNA Wash Solution I (spin column) DNase I treatment RNA Wash Solution I RNA Wash Solution II (2 times) DESCRIPTION Cat.No. REACTION Plant Total RNA Purification Kit TR02 50 Plant Total RNA Purification Kit TR Elute Ultrapure plant total RNA GeneMark 32

4 GeneMark GMbiolab Co., Ltd. Purity: Unique DNase I to remove genomic DNA contamination. Unique RNase-free Filtration s CS provides RNA free from other contaminations. RNA can be obtained in 30~40 minutes. Yield: 2~3 μg for 500 ml blood sample. No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradients centrifugation. GM RNAprep pure Blood Purification Kit GM RNAprep Pure Blood Purification Kit provides a fast, simple, and costeffective method for purification of total RNA from blood samples using effective spin column and unique buffer system. The simplicity of the procedure allows rapid parallel processing of multi-sample total RNA purification. High-quality total RNA can be obtained within 30~40 minutes and is free from protein and other contaminations. Northern blotting, dot blot, real-time PCR, RT-PCR, in vitro translation, poly(a) screening, Chip analysis. Supplier A GM Figure 1. Total RNA purified from blood using RNA purification products from GM RNAprep pure Blood od Purification Kit and Supplier A. Total RNA was purified from the blood of object S (S) and object L (L). 500 ng of purified RNA was loaded per lane for gel electrophoresis analysis. DESCRIPTION Cat.No. REACTION GM RNAprep Pure Blood Purification Kit GDP GeneMark

5 High-quality viral total RNA can be obtained within an hour. Sample Volume: 140 μl~560 μl liquid sample is accepted. GMamp Virus RNA Kit GMamp Virus RNA Kit provides a fast, simple, and cost-effective viral RNA miniprep method for viral RNA purification from whole blood, plasma, serum and other cell-free materials. GMamp Virus RNA Kit uses silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Carrier RNA in the kit improves the binding of viral RNA to the GMamp membrane especially in the case of low-titer samples, and limits possible degradation of the viral RNA due to any residual RNase activity. Viral RNA purified using GMamp Virus RNA Kit is immediately ready for use. Phenol extraction and ethanol precipitation are not required. High-quality viral RNA is eluted in a small volume of RNase-free ddh 2 O. Viral (HCV) genotyping research, viral epidemiologic study, viral infectious diseases analysis. No phenol/chloroform extraction and no CsCl gradients, no LiCl or ethanol precipitation. A B Sample Duplicate CT value Average Sample Duplicate CT value Average 1 Duplicate Duplicate Duplicate Duplicate Duplicate Duplicate Duplicate Duplicate Duplicate Duplicate Duplicate Duplicate NTC None - Average NTC None - Average Figure 1. Virus Genomic DNA purified using GMamp Virus DNA/RNA A Kit. Genomic DNA was purified form 200 μl of Avian influenza virus standard antigen that was diluted 100 times (A), or 10,000 times (B). 4 μl of 50 μl eluates were used as template in real-time PCR detection. Real-time PCR were in triplicate for each sample. DESCRIPTION Cat.No. REACTION GMamp Virus RNA Kit GDP315-R 50 GeneMark 34

6 GeneMark GMbiolab Co., Ltd. GMamp Virus DNA/RNA Kit Fast: High-quality viral genomic DNA or total RNA can be obtained within an hour. Sample Volume: 200 μl liquid sample is accepted. GMamp virus DNA / RNA kit provides a fast, simple, and cost-effective virus DNA/RNA miniprep method for virus DNA or RNA purification from plasma, serum and other cell-free materials. GMamp virus DNA / RNA kit is based on silica membrane technology and unique buffer system. Carrier RNA in the kit improves the binding of virus RNA to the membrane especially in the case of low-titer samples, and limits possible degradation of the virus RNA due to any residual RNase activity. High-quality virus DNA / RNA is eluted in a small volume of Rnase-free ddh 2 O. The purified DNA/RNA is ready for use in subsequent applications such as PCR, RT-PCR, real-time PCR, nest PCR, etc. Viral (HCV) genotyping research, viral epimiologic study, viral infectious diseases analysis. No phenol/chloroform extraction and no CsCl gradients, no LiCl or ethanol precipitation. Figure 1. Viral DNA purified using GMamp Virus DNA/RNA A Kit. Viral DNA was purified form serum samples containing Hepatitis E virus 5 x 10 6, 5 x 10 5, 5 x 10 4, 5 x 10 3 copies/ml. Purified performance was analyzed by qrt-pcr detection. DESCRIPTION Cat.No. REACTION GMamp Virus DNA/RNA kit GDP GeneMark

7 Rapid purification of high quality, ready-to-use viral DNA and RNA within 45 minutes. No phenol / chloroform extraction. GM Magnetic Viral DNA/RNA Kit GM Magnetic Viral DNA/RNA Kit is designed for the rapid and reliable purification of viral RNA and viral DNA from serum, plasma and body fluids. Special magnetic beads technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. Nucleic acids (DNA/RNA) will form a complex with magnetic beads in a specially formulated buffer. The beads/nucleic acids complex is separated from lysates using a magnet. Purified DNA/RNA are then eluted when adjust the buffer condition. RT-PCR, Real-time RT-PCR Figure 1. qrt-pcr R result of purified RNA Total RNA was extracted from Bird Flu Standard Antigen (gradient diluted by 50, 100, 200, 400, 800, 1600, 3200, 6400 and 12800) and Bird Flu viral specific primers and probed were used to carry out qrt-pcr. DESCRIPTION Cat.No. Package GM Magnetic Viral DNA/RNA Kit GDP GeneMark 36

8 GeneMark GMbiolab Co., Ltd. Convenient: High-quality DNA and RNA from the same sample. The kit allows the parallel processing of multiple samples in 40~50 minutes. No phenol/chloroform extraction or ethaol precipitation. GMprep DNA/RNA Isolation Kit GMprep DNA/RNA Isolation Kit is designed for purifying both genomic DNA and total RNA from the same cell or tissue sample. The kit is compatible with a wide range of animal cells and tissues. A simple workflow allows the purification of both high-quality DNA and RNA from the same sample in only 40~50 minutes. The purified DNA and RNA are eluted separately and ready to use in any downstream application. RT-PCR, Real-time RT-PCR DESCRIPTION Cat.No. Package GMprep DNA/RNA Isolation Kit GDP Purity: Unique DNase I to digest genomic DNA. No phenol / chloroform extraction or ethanol precipitation. GMprep Mini pure RNA Kit GMprep Mini pure RNA Kit is designed for purification of total RNA from small samples. After genomic DNA removal, high-quality RNA is purified using RNase-free CR1. High quality total RNA can be ready in 30~40 minutes. RT-PCR and real-time RT-PCR, differential display, in vitro translation, poly(a) screening, cdna synthesis, Northern dot and slot blot analysis. Figure 1. qrt-pcr result of purified RNA Total RNA extracted from Hela cells (1 X 10 6, 1 X 10 5, 1 X 10 4, 1 X 10 3, 1 X 10 2, 10) using GMprep pure Micro RNA Kit. DESCRIPTION Cat.No. Package GMprep Mini pure RNA Kit GDP GeneMark

9 GM mircute micro RNA Isolation Kit Efficiency: Efficient enrichment of micro RNA and RNAs <200 bp. RNA can be obtained within an hour. GM mircute micro RNA Isolation Kit is uniquely designed to purify cellular small (20~200 nt) RNA molecules, including mirna, sirna and snrna. The kit can also be used to purify total RNA from samples. The kit utilizes a silicabased two-column system to enrich small RNA from various sample sources. From sample lysate, RNAs greater than 200 bp are bound to the first column. The flow-through, enriched with small RNA molecules, is mixed with a higher concentration of ethanol to increase the binding affinity of small RNA to the membranes of the second column. Bound small RNA molecules are then eluted, ready-to-use, and free of large RNA molecules that can inhibit expression analysis of small RNA molecules. mirna related analysis. Figure 1. qrt-pcr result of purified mirna Figure 2. Purification Comparison of mirna DESCRIPTION Cat.No. Package GM mircute micro RNA Isolation Kit GDP GeneMark 38

10 GeneMark GMbiolab Co., Ltd. Convenient: Suitable for a wide range of sample types and sample sizes. Economic: One solution for purification of RNA, DNA and proteins. TriSolution Plus Reagent TriSolution Plus Reagent is a mixture of phenol, guanidium thiocynate, buffers and stabilizers developed for the simultaneously isolation of total RNA, DNA and proteins from animal and plant tissues, cells and bacteria culture. The entire procedure for total RNA isolation is an improved single-step method developed by Chomczynski and Sacchi. After homogenization of sample and chloroform extraction, three phases are formed (aqueous phase, interphase and organic phase). RNA can be precipitated by isopropanol from aqueous phase, DNA can be recovered by ethanol precipitation from interphase, and proteins are precipitated with isopropanol from organic phase. The reagent also includes a bottle of PS & PG Removal Solution, which can eliminate polysaccharides and proteoglycans contamination, by which the RNA can be dissolve easier and purer for RT-PCR and northern blot application. RNA isolated can be used for general molecular experiments such as Northern analysis, dot hybridization, poly A+ selection, in vitro translation, RNase protection assays, RT-PCR Figure1. RNA purified from rat kidney using TriSolution Plus Reagent. Total RNA was purified from rat kidney stored under different conditions. Lane 1: stored at 37 for 24 hr in RNAfter (GeneMark Cat.No.: RA100) Lane 2: stored at 4 for 30 days in RNAfter. Lane 3: stored at -70 for 30 days without RNAfter. DESCRIPTION Cat.No. Size TriSolution Plus Reagent TS-100-Plus 100 ml TriSolution Plus Reagent TS-200-Plus 200 ml 39 GeneMark

11 Efficient: Performs well with small quantities of tissue (50~100 mg)and cells ( ), and large quantities of tissue (>1 g) and cells (>10 7 ), of human, animals, plants, or bacterial origins. GRNzol Reagent GRNzol Reagent is a ready-to-use reagent for the purification of total RNA from cells and tissues. The reagent is a monophasic solution of phenol and guanidine isothiocyanate. During sample homogenization or lysis, GRNzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Total RNA isolated by GRNzol Reagent is free of protein and DNA contamination. Northern blot analysis, RT-PCR, dot blot hybridization, and molecular cloning. M Sample Sample Size Yield Range Wheat 1 g µg Mouse liver 1 mg 6 10 µg Cultured cells µg E. coli 1 ml 2 10 µg Whole blood 1 ml 3 5 µg Figure 1. Total RNA extracted using GRNzol and related product from supplier A. Total RNA was extracted from Human Jurkat cells. GRNzol yields a higher quantity of high-purity total RNA. Lane 1, 2, 3: RNA extracted using GRNzol Reagent Lane 4, 5, 6: RNA extracted using related product from supplier A. M: Marker DESCRIPTION Cat.No. SIZE GRNzol Reagent GDP405-Try 20 ml GRNzol Reagent GDP ml GRNzol Reagent GDP ml GeneMark 40

12 GeneMark GMbiolab Co., Ltd. RNA can be obtained within an hour. Yield: Higher RNA yields than GRNzol Reagent. GRNzol -A+ Reagent GRNzol A+ Reagent is designed in the base of GRNzol Reagent. The reagent is optimized for isolation of RNA from cell cultures and tissues. The GRNzol A+ Reagent is optimized for lysis of a variety of sample types before RNA isolation with standard homogenization methods and alcohol precipitation. This reagent performs well with small quantities of tissue (50~100 mg) and cells ( ), and large quantities of tissue (>1 g) and cells (>10 7 ), of human, animals, plants, or bacterial origins. Northern blotting, qrt-pcr, poly(a) RNA selection, and RNase protection assays. Figure 1. Real-time PCR results of HPV virus RNA using GRNzol-A+ Reagent 10-fold serial dilutions of total HPV virus RNA (10 2 ~10 5 copies/ml) were quantified by qrt-pcr. Figure 2. Total RNA purified using GRNzol-A+ and related product from supplier A. Total RNA of higher quality was obtained using GRNzol-A+ Reagent. GRNzol-A+ Supllier A DESCRIPTION Cat.No. Size GRNzol-A+ Reagent GDP421-Try 20 ml GRNzol-A+ Reagent GDP ml GRNzol-A+ Reagent GDP ml 41 GeneMark