UltraClean BloodSpin DNA Isolation Kit

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1 UltraClean BloodSpin DNA Isolation Catalog No. Quantity Preps Preps Instruction Manual Please recycle Version:

2 Table of Contents Introduction... 3 Protocol Overview... 3 Flow Chart... 4 Equipment Required... 5 Contents & Storage... 5 Precautions & Warnings... 5 Protocols: Protocol...6 Detailed Protocol (Describes what is happening at each step)...7 Vacuum Manifold Protocol...9 Hints & Troubleshooting Guide...11 Contact Information...13 Other Quality Products Available

3 Introduction The UltraClean BloodSpin DNA Isolation is designed to rapidly isolate genomic and mitochondrial DNA from whole blood (fresh, frozen or stored at 4 C), buffy coat, swabs, or cultured cells. Use this kit for isolating Genomic DNA from 200 µl of whole blood or liquid body fluid, or 20 μl of avian blood. This spin filter kit yields PCR ready DNA. Protocol Overview UltraClean silica spin column products utilize the novel MO BIO Laboratories flat bottom spin column design, which provides improved sample processing and yields. The bucket configuration allows for enhanced sample flow and membrane drying after wash steps since the entire membrane is accessible to air flow. Silica technology provides a robust and fast way to purify nucleic acids without the use of organic solvents or cesium chloride gradients. Purification of DNA is performed with 200 µl of fluid solution (whole blood, swab lysate, cells or body fluids) mixed with proteinase K and a chaotropic salt solution that enhances lysis of cells and capture of DNA to the silica filter membrane. The sample is heated for 10 minutes and then mixed with ethanol to optimize the binding conditions for DNA to silica using centrifugation. The membrane is washed with solutions that remove proteins and salt, and DNA is eluted in 10 mm Tris 1mM EDTA ph 8.5 buffer and is ready to use in downstream applications including PCR and real-time PCR. High Throughput Options MO BIO offers a vacuum based protocol for faster processing without centrifugation for the DNA binding and column washing steps for Spin Filters. The MO BIO PowerVac Manifold allows for processing of up to 20 spin filter preps at a time using the PowerVac Mini Spin Filter Adapters. The UltraClean -htp 96 Well BloodSpin DNA Isolation is available for processing up to 2 x 96 samples using a centrifuge capable of spinning two 96 Well Blocks stacked (13 cm x 8 cm x 5.5 cm) at 2500 x g. This kit is for research purposes only. Not for diagnostic use. Other Related Products Catalog No. Quantity BiOstic Bacteremia DNA Isolation PowerVac Manifold manifold PowerVac Mini System unit + 20 adapters PowerVac Mini Spin Filter Adapters adapters 20 adapters 3

4 4

5 Equipment Required Microcentrifuge (13,000 x g) 65 C water bath Pipettor (volumes required 10 l l), vortex Optional Reagents RNase A (MO BIO Catalog# ; 1 ml) Reagents Required but not Included 100% ethanol and 70% ethanol (for the PowerVac Manifold protocol only) Contents Catalog# Catalog# Component Catalog# Amount Catalog# Amount Proteinase K Solution PKS 550 μl PKS 2.75 ml Solution B ml ml Solution B ml ml Solution B ml x 28 ml Solution B ml x 30 ml Solution B ml ml Spin Filters SF SF ml Collection Tubes T T 750 Storage The Proteinase K is stable at room temperature, however, for long term storage we recommend 4 C. All other kit reagents should be stored at room temperature (15-30 C). Precautions Please wear gloves when using this product. Avoid all skin contact with reagents in this kit. In case of contact, wash thoroughly with water. Do not ingest. See Material Safety Data Sheets for emergency procedures in case of accidental ingestion or contact. All MSDS information is available upon request ( ) or on our web site at Reagents labeled flammable should be kept away from open flames and fire. The Center for Disease Control, the Food and Drug Administration, and the American Hospital Association recommend universal precautions when working with blood and body fluids. To prevent contact with potentially infectious pathogens, it is recommended that workers protect themselves from contact with these fluids by using suitable barrier protection which includes gloves. Effective barrier protection should be used during this procedure. WARNING: Solution B2, B3, and B4 are flammable. Avoid contact with bleach or other oxidizers. Do not use bleach to clean the inside of the PowerVac Manifold or to rinse the PowerVac Mini Spin Filter Adapters when attached to the manifold. 5

6 Protocol Please wear gloves at all times 1. Add 200 μl of whole blood or body fluids to a 2 ml Collection Tube (provided) and add 10 µl of Proteinase K. Note: (Optional: RNase A can be added at this step. Use 2 µl of 20 mg/ml.) 2. Add 200 µl of Solution B1 and mix by vortexing for 15 seconds. 3. Incubate the sample at 65 C for 10 minutes. Centrifuge briefly to collect the lysate. 4. Add 200 µl of Solution B2 and vortex for 15 seconds. Centrifuge briefly to collect the sample from the lid. 5. Transfer the lysate to the Spin Filter and centrifuge for 1 minute at 13,000 x g. 6. Transfer Spin Filter to new 2 ml Collection Tube (provided). 7. Add 500 µl of Solution B3 to Spin Filter. Centrifuge for 30 seconds at 13,000 x g 8. Remove the Spin Filter and discard the flow through. Place Spin Filter back into the same 2 ml Collection Tube. 9. Add 500 µl of Solution B4 to Spin Filter. Centrifuge 30 seconds at 13,000 x g. 10. Remove the Spin Filter and discard the flow through. Place Spin Filter back into the same 2 ml Collection Tube. 11. Centrifuge again for 30 seconds at 13,000 x g to dry the Spin Filter membrane. 12. Carefully remove Spin Filter and transfer to a new 2 ml Collection Tube (provided). 13. Add µl of Solution B5. Note: To increase yields, incubate for 5 minutes at 65 C. 14. Centrifuge 1 minute at 13,000 x g. 15. Remove Spin Filter unit and close tube lid. Genomic DNA in tube is now ready to use for any application. Thank you for choosing the UltraClean BloodSpin DNA Isolation. 6

7 Detailed Protocol (Describes what is happening at each step) Please wear gloves at all times 1. Add 200 μl of whole blood or body fluids to a 2 ml Collection Tube (provided) and add 10 µl of Proteinase K. Note: (Optional: RNase A can be added at this step. Use 2 µl of 20 mg/ml.) What s happening: Proteinase K breaks down the cell wall and helps lyse the cells. To ensure complete removal of RNA, add RNase A at this step. No incubation is necessary. 2. Add 200 µl of Solution B1 and mix by vortexing for 15 seconds. What s happening: Solution B1 is a lysis reagent. It contains a chaotropic salt and detergent. 3. Incubate the sample at 65 C for 10 minutes. Centrifuge briefly to collect the lysate. What s happening: Heat helps denature proteins and completes lysis. 4. Add 200 µl of Solution B2 and vortex for 15 seconds. Centrifuge briefly to collect the sample from the lid. What s happening: Solution B2 contains 100% ethanol and provides optimal conditions for DNA binding. 5. Transfer the lysate to the Spin Filter and centrifuge for 1 minute at 13,000 x g. What s happening: DNA binds to the silica membrane in the spin filter because it is in a high salt condition. The liquid flow through contains unwanted cell material such as denatured proteins and RNA.. 6. Transfer Spin Filter to new 2 ml Collection Tube (provided). 7. Add 500 µl of Solution B3 to Spin Filter. Centrifuge for 30 seconds at 13,000 x g What s happening: Solution B3 is a salt based wash solution that cleans the DNA bound to the Spin Filter. 8. Remove the Spin Filter and discard the flow through. Place Spin Filter back into the same 2 ml Collection Tube. 9. Add 500 µl of Solution B4 to Spin Filter. Centrifuge 30 seconds at 13,000 x g. What s happening: Solution B4 is an ethanol based wash solution that cleans the DNA bound to the Spin Filter. It removes all residual salt contaminants. 10. Remove the Spin Filter and discard the flow through. Place the Spin Filter back into the same 2 ml Collection Tube. 11. Centrifuge again for 30 seconds at 13,000 x g to dry the Spin Filter membrane. What s happening: The Spin Filter is dried of ethanol to allow for maximal DNA release from the Spin Filter membrane during elution. 12. Carefully remove Spin Filter and transfer to a new 2 ml Collection Tube (provided). 13. Add µl of Solution B5. Note: To increase yields, incubate for 5 minutes at 65 C. 7

8 14. Centrifuge 1 minute at 13,000 x g. 15. Remove Spin Filter unit and close tube lid. Genomic DNA in tube is now ready to use for any application. What s happening: Solution B5 is 10mM Tris 1mM EDTA. As it passes through the Spin Filter, the DNA is released (eluted) off the filter and it passes into the 2 ml Collection Tube. The DNA is released because it will not stay bound to the Spin Filter when there is no salt present. Thank you for choosing the UltraClean BloodSpin DNA Isolation. 8

9 Vacuum Protocol using the PowerVac Manifold Please wear gloves at all times For each sample lysate, use one Spin Filter column. Keep the Spin Filter in the attached 2 ml Collection Tube and continue using the Collection Tube as a Spin Filter holder until needed for the Vacuum Manifold Protocol. Label each Collection Tube top and Spin Filter column to maintain sample identity. If the Spin Filter becomes clogged during the vacuum procedure, you can switch to the procedure for purification of the DNA by centrifugation. You will need to provide 70% ethanol for step 4 and 5 of this protocol and 100% ethanol for step For each prep, attach one aluminum PowerVac Mini Spin Filter Adapter (MO BIO Catalog# or ) into the Luer-Lok fitting of one port in the manifold. Gently press a Spin Filter column into the PowerVac Mini Spin Filter Adapter until snugly in place. Ensure that all unused ports of the vacuum manifold are closed. Note: Aluminum PowerVac Mini Spin Filter Adapters are reusable. 2. Transfer 650 l of prepared sample lysate (from step 5) to the Spin Filter column. 3. Turn on the vacuum source and open the stopcock of the port. Hold the tube in place when opening the stopcock to keep the spin filter steady. Allow the lysate to pass through the Spin Filter column. Continue until all of the lysate has been loaded onto the Spin Filter column. Close the one-way Luer- Lok stopcock of that port. Note: If Spin Filter Columns are filtering slowly, close the ports to samples that have completed filtering to increase the pressure to the other columns. 4. Load 800 l of 70% ethanol into the Spin Filter so that it completely fills the column. Open the stopcock while holding the column steady. Allow the ethanol to pass through the column completely. Close the stopcock. 5. Repeat step Load 500 l of Solution B3 into the Spin Filter. Open the stopcock and allow the wash solution to pass through the column completely. Close the stopcock. 7. Load 500 l of Solution B4 into the Spin Filter. Open the stopcock and allow the wash solution to pass through the column completely. Close the stopcock. 8. Load 800 l of 100% ethanol into the Spin Filter so that it completely fills the column. Open the stopcock while holding the column steady. Allow the ethanol to pass through the column completely. Close the stopcock. 9. Turn off the vacuum source and open an unused port to vent the manifold. If all 20 ports are in use, break the vacuum at the source. Make certain that all vacuum pressure is released before performing the next step. It is important to turn off the vacuum at the source to prevent backflow into the columns. 10. Remove the Spin Filter column and place in the original labeled 2 ml Collection Tube. Place into the centrifuge and spin at 13,000 g for 1 minute to completely dry the membrane. 9

10 11. Transfer the Spin Filter column to a new 2 ml Collection Tube and add 100 l of Solution B5 to the center of the white filter membrane. 12. Centrifuge at room temperature for 30 seconds at 10,000 x g. 13. Discard the Spin Filter column. The DNA in the tube is now ready for any downstream application. No further steps are required. We recommend storing DNA frozen (-20 to -80 C). Solution B5 contains 10 mm Tris, 1mM EDTA. To concentrate the DNA see the Hints & Troubleshooting Guide. Thank you for choosing the UltraClean BloodSpin DNA Isolation. 10

11 Hints and Troubleshooting Guide Clogging of the Silica Spin Filter When using 200 μl of normal human blood, the silica membrane will not clog when following the protocol as directed. If clogging does occur, the following may be the reason: Lysis may have been incomplete. Make sure to mix well after adding Solution B1 and ensure that the Proteinase K Solution was added. Check that the digestion temperature was set correctly. Do not use more than 200 μl of human blood and not more than 20 μl of avian blood. For other animal species, start with 100 μl of blood if you are unsure of the expected yields or white blood cell count. Blood must be treated with anti-coagulants before processing to avoid blood clots. Do not use with clotted blood. If blood contains clots, remove the clots and use only the liquid portion of the sample. Low DNA Yield The average yield for 200 μl of human blood is 4-6 μg of DNA. DNA yields may be lower if the sample has been stored for an extended period of time or has been freeze/thawed multiple times. The following may also apply if yields are below average: A low yield may occur if the lysis is not complete. Make sure to mix well after adding Solution B1 and ensure that the Proteinase K Solution was added. Check that the digestion temperature was set correctly. Do not pre-mix the Proteinase K Solution and the Solution B1 before using. Proteinase K activity may be reduced. Do not skip the Solution B4 wash step. Incomplete removal of salt will prevent release of DNA from the silica filter membrane. DNA has Low A 260/280 Ratio The ratio for pure DNA should be A 260/280 reading below 1.6 may have significant protein contamination. Low A 260/280 ratios may be caused by the following: Make sure to perform the Solution B3 wash before the Solution B4 wash to remove protein contaminants. If using a Nanodrop, blank the instrument with Solution B5. A low ratio may also occur when the sample is measured by UV spectrophotometry in water. The low ph of water can influence the 280 reading and cause reduced sensitivity to protein contamination(1). Re-measure the 260/280 diluting the RNA for measurement in 10 mm Tris ph *Wilfinger, W.W., Mackey, M., and Chomczynski, P. (1997) Effect of ph and ionic strength on the spectrophotometric assessment of nucleic acid purity. BioTechniques 22, 474. DNA Floats Out of Well When Loaded on a Gel This usually occurs because residual Solution B4 remains in the final sample. Do not skip the final dry spin centrifugation step and you may extend the dry spin to 1-2 minutes if ethanol removal is a problem due to humidity in your lab. Ethanol precipitation (described in Concentrating the DNA ) is the best way to remove residual Solution B4. 11

12 Hints and Troubleshooting Guide cont. Concentrating the DNA The final volume of eluted DNA will be 200 μl. The DNA may be concentrated by adding 8 μl of 5M NaCl and inverting 3-5 times to mix. Next, add 400 μl of 100% cold ethanol and invert 3-5 times to mix. Centrifuge at 13,000 x g for 15 minutes at room temperature. Decant all liquid. (If sterile DNA is desired, wash the DNA pellet with 70% cold ethanol. Be sure not to disturb the pellet.) Remove residual ethanol in a speed vac, dessicator, or ambient air. Resuspend precipitated DNA in sterile water or TE buffer. Cleaning of the PowerVac Mini Spin Filter Adapters It is recommended to rinse the PowerVac Mini Spin Filter Adapters promptly after use to avoid salt build up. To clean the PowerVac Mini Spin Filter Adapters, rinse each adapter with DI water followed by 70% ethanol and flush into the manifold base. Alternatively, remove the adapters and wash in laboratory detergent and DI water. PowerVac Mini Spin Filter Adapters may be autoclaved. Do not use bleach to clean the PowerVac Mini Spin Filter Adapters while attached to the PowerVac Manifold. Bleach should never be mixed with solutions containing guanidine and should not be used to clean the PowerVac Manifold. For more information on cleaning the PowerVac Manifold, please refer to the PowerVac Manifold manual. 12

13 Contact Information Technical Support: Phone MO BIO Laboratories, Inc. Toll Free , or Fax: Mail: MO BIO Laboratories, Inc, 2746 Loker Ave West, Carlsbad, CA Ordering Information: Direct: Phone MO BIO Laboratories, Inc. Toll Free , or Fax: Mail: MO BIO Laboratories, Inc, 2746 Loker Ave West, Carlsbad, CA For the distributor nearest you, visit our web site at 13

14 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to to request a catalog. DNA Purification and Gel Extraction Catalog No. Quantity RNA Isolation Continued Catalog No. Quantity PowerClean DNA Clean-Up UltraClean Plant RNA Isolation preps UltraClean 15 DNA Purification preps Genomic DNA Isolation Catalog No. Quantity UltraClean PCR Clean-Up preps PowerFood Microbial DNA Isolation preps UltraClean -htp 96 Well PCR Clean- Up x 96 preps 12 x 96 preps BiOstic Bacteremia DNA Isolation UltraClean GelSpin DNA Extraction preps 2 Plasmid DNA Isolation Catalog No. Quantity UltraClean 6 Minute Mini Plasmid Prep preps UltraClean Standard Mini Plasmid Prep preps 2 4 x 96 preps 12 x 96 preps 20 preps 10 preps 20 preps 100 preps 2 BiOstic FFPE Tissue DNA Isolation BiOstic Paraffin Removal Reagent x 25 ml PowerMax Soil DNA Isolation preps PowerSoil DNA Isolation preps UltraClean -htp 96 Well Plasmid Prep PowerSoil -htp 96 Well Soil DNA Isolation x 96 preps 12 x 96 preps UltraClean Midi Plasmid Prep UltraClean Soil DNA Isolation preps UltraClean Maxi Plasmid Prep UltraClean -htp 96 Well Soil DNA x 96 preps Isolation x 96 preps UltraClean Endotoxin-Free Mini UltraClean Mega Soil DNA Isolation preps Plasmid Prep UltraClean Endotoxin-Free Midi preps PowerClean DNA Clean-Up Plasmid Prep UltraClean Endotoxin-Free Maxi preps UltraClean Fecal DNA Isolation Plasmid Prep preps UltraClean Endotoxin Removal kit PowerMicrobial Midi DNA Isolation preps UltraClean Endotoxin-Free Ethanol kit PowerMicrobial Maxi DNA Isolation preps Precipitation UltraClean Endotoxin Removal ml UltraClean Microbial DNA Isolation Reagent Endotoxin-Free Sodium Chloride ml UltraClean -htp 96 Well Microbial x 96 preps DNA Isolation x 96 preps Endotoxin-Free Centrifuge Tubes each/2 ml PowerPlant DNA Isolation preps each/15 ml each/50 ml RNA Isolation Catalog No. Quantity LifeGuard Soil Stabilization Solution ml ml L L On-Spin Column DNase I (RNase- Free) BiOstic Stabilized Blood RNA Isolation BiOstic Blood Total RNA Isolation RNA PowerSoil DNA Elution Accessory RNA PowerSoil Total RNA Isolation UltraClean Microbial RNA Isolation UltraClean Tissue & Cells RNA Isolation UltraClean Plant DNA Isolation UltraClean -htp 96 Well Plant DNA Isolation UltraClean Tissue & Cells DNA Isolation preps UltraClean -htp 96 Well Tissue DNA Isolation preps preps UltraClean Blood DNA Isolation (Non-Spin) preps UltraClean Blood DNA Isolation (Processes 1,000 ml of Blood) preps UltraClean Blood DNA Isolation Plus RNase (Processes 1,000 ml of Blood) UltraClean BloodSpin DNA Isolation UltraClean -htp 96 Well BloodSpin DNA Isolation x 96 preps 12 x 96 preps 2 4 x 96 preps 12 x 96 preps preps kit kit x 96 preps 12 x 96 preps 14

15 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to to request a catalog. Genomic DNA Isolation Continued Catalog No. Quantity UltraClean Forensic DNA Isolation isolations isolations PowerWater DNA Isolation NF RapidWater DNA Isolation UltraClean Water DNA Isolation (0.45µm filters) UltraClean Water DNA Isolation (0.22 µm filters) UltraClean Water DNA Isolation (No filters) NF NF NF NF NF (No filters) (0.22 μm) (0.45 μm) 100 preps (No filters) (0.22 μm) (0.45 μm) (No filters) (0.22 μm) (0.45 μm) 100 preps (No filters) (0.22 μm) (0.45 μm) 10 preps 25 preps 10 preps 25 preps 10 preps 25 preps Microbiological Culture Media Catalog No. Quantity TB DRY Powder Growth Media LB Broth Powder Growth Media, ph LB Agar Powder Growth Media, ph LB Broth (Lennox) Powder Growth Media, ph 7 LB Agar (Lennox) Powder Growth Media, ph 7 Soybean-Casein Digest Medium (TSB), USP Soybean-Casein Digest Agar Medium (TSA), USP Yeast Extract Tryptone Agar, Bacteriological Grade Other Reagents and Lab Other Reagents and Lab Accessories Continued Catalog No. Quantity 100 bp DNA Ladder µg 1 kb DNA Ladder µg UltraClean Agarose, Molecular Biology Grade UltraClean MS-8 Agarose UltraClean Forensic Agarose UltraClean Low Melt Agarose UltraClean Low Melt Sieve Agarose Ethidium Bromide Solution Ethidium Bromide Destaining Tea Bags Bromophenol Blue Gel Loading Buffer Bromophenol Blue/Xylene Cyanol Gel Loading Buffer g 100 g 50 g 100 g 50 g 100 g 50 g 100 g 50 g 100 g 1 ml 10 ml bags TAE Buffer, 50X (Tris-acetate-EDTA) TBE Buffer, 10X (Tris-borate-EDTA) RNase-Free Gloves 1555-XS 1555-S 1555-M 1555-L UltraClean Lab Cleaner OmniTaq DNA Polymerase Enzyme OmniTaq DNA Polymerase 2x Master Mix Accessories Catalog No. Quantity Omni KlenTaq DNA Polymerase Enzyme 20 bp DNA Ladder µg Omni KlenTaq DNA Polymerase 2x Master Mix ml 5 x 1 ml 1 ml 5 x 1 ml 100 ml 500 ml 1 liter 100 ml 500 ml 1 liter bag of 100 bag of 100 bag of 100 bag of ml squeeze bottle 500 ml spray bottle 1 liter bottle reactions (10 U/µl) reactions (5 x 1.25 ml/tube) reactions (25 U/µl) reactions (5 x 1.25 ml/tube)

16 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to to request a catalog. Other Reagents and Lab Accessories Continued Catalog No. Quantity DNase (RNase-Free) mg units Proteinase K Ribonuclease A (25 mg/ml) PCR Water Molecular Biology Grade Water DEPC Treated Water mg 2 ml (20 mg/ml) 1 ml 5 ml 1 ml 5 x 1 ml 10 x 1 ml 10 ml bottle 200 ml 5 x 200 ml 200 ml 5 x 200 ml Instrumentation and Accessories Continued Catalog No. Quantity Glass 50 ml Bead Tubes, 0.1 mm Glass 15 ml Bead Tubes, 1.0 mm Ceramic 15 ml Bead Tubes, 1.4 mm Ceramic 50 ml Bead Tubes, 1.4 mm Metal 50 ml Bead Tubes, 2.38 mm PowerMix 15 ml Bead Tubes Endotoxin-Free Water ml 50 ml 100 ml 500 ml PowerMix 50 ml Bead Tubes ml Collection Tubes T T T Instrumentation and Accessories Catalog No. Quantity BagMixer 400 VW unit 2 ml Screw Cap Tubes E 200 & caps BagFilter 400 P Box of ml Collection Tubes T 25 BagPage Box of ml Centrifuge Tubes T 25 Precellys 24 Homogenizer, 120V unit Spin Filters (in 1.9 ml ) SF SF SF Ceramic Bead Tubes, 1.4 mm bead Endotoxin-Free Centrifuge Tubes filters 100 filters 250 filters 100 each/2 ml 50 each/15 ml 25 each/50 ml Ceramic Bead Tubes, 2.8 mm bead 15 ml Midi Spin Filters SF 25 spin filters Glass Bead Tubes, 0.5 mm bead Vortex-Genie 2 Vortex (120V) V 1 unit Glass Bead Tubes, 0.1 mm bead Vortex-Genie 2 Vortex (220V) V unit Metal Bead Tubes, 2.38 mm bead Vortex Adapter, holds 12 ( ml) V1 1 unit 2.0 ml Tough Tubes with Cap Vortex Adapter, holds 6 (5 ml) V1-5 1 unit Carbide Bead Tubes, 0.25 mm x 0.5 ml Vortex Adapter, holds 4 (15 ml) V unit Garnet Bead Tubes, 0.15 mm x 0.5 ml Vortex Adapter, holds 2 (50 ml) V unit Garnet Bead Tubes, 0.70 mm x 2 ml Vortex Adapter, holds 24 ( ml) V unit Garnet + ¼ Ceramic 15 ml Bead Power Supply w/timer, (120V) unit Tubes, 0.70 mm Garnet + ¼ Ceramic 50 ml Bead Tubes, 0.70 mm ` Power Supply w/timer, (220V) unit Glass 15 ml Bead Tubes, 0.1 mm Polycarbonate Single-sided Comb mm x 3 well 1 mm x 8 well 1 mm x 10 well 1 mm x 12 well 16

17 Other Quality Products Available from MO BIO Laboratories, Inc. For more product and detailed information go to to request a catalog. Instrumentation and Accessories Continued Catalog No. Quantity Polycarbonate Dual-sided Comb mm x 8 well/16 well mm x 10 well/14 well mm x 8 well/16 well mm x 10 well/14 well Teflon Single-sided Comb mm x 3 well mm x 8 well mm x 10 well mm x 12 well Teflon Dual-sided Comb mm x 8 well/16 well mm x 10 well/14 well mm x 8 well/16 well mm x 10 well/14 well Instrumentation and Accessories Continued Catalog No. Quantity 96 Well Plate Shaker (220V) unit Plate Adapter Set set Tube Adapter Set set Mini Horizontal Gel System each Vacuum Pump (120V) unit Mini Horizontal Gel Caster, 3 place each Vacuum Pump (220V) unit Mini Horizontal Gel Tray each UltraVac Manifold unit 96 Well Plate Shaker (120V) unit 17

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