PureSpin. Gel DNA Purification Kit. Micro Columns INSTRUCTION MANUAL. KIT COMPONENTS For Research Use Only. PureSpin.

Size: px
Start display at page:

Download "PureSpin. Gel DNA Purification Kit. Micro Columns INSTRUCTION MANUAL. KIT COMPONENTS For Research Use Only. PureSpin."

Transcription

1 PureSpin Gel DNA Purification Kit Micro Columns INSTRUCTION MANUAL KIT COMPONENTS For Research Use Only PureSpin Gel DNA Purification Kit, Micro Columns w/out Caps (Kit Size) OD2030 (50 Preps) OD (200 Preps) Storage Temperature Agarose Dissolving Buffer 50ml 2x100ml Room Temperature DNA Wash Buffer 1 (concentrate) 6ml 24ml Room Temperature DNA Elution Buffer 1ml 4ml Room Temperature DNA PureSpin Micro Columns Room Temperature Collection Tubes Room Temperature

2 FEATURES Quick, 15 minute high-yield recovery of ultra-pure DNA from agarose gels. Innovative PureSpin Micro Column design. Minimal elution volumes ( 6μl) and complete sample recovery with no carryover. Eluted DNA is well suited for use in DNA ligation, sequencing, labeling, PCR, etc. CONTENTS Contents p. 2 Specifications p. 3 Product Description p. 4 Buffer Preparation p. 5 Protocols p. 5 Troubleshooting p. 6 Ordering Information p. 8 Related Products p. 8 Notes from the cover: Integrity of kit components is guaranteed for up to one year from date of purchase. Reagents are routinely tested on a lot-to-lot basis to ensure they provide the highest performance and reliability. 1 Ethanol must be added prior to use as indicated on the DNA Wash Buffer label. Page 2

3 SPECIFICATIONS Sample Sources: DNA Purity: DNA Size Limits: DNA Recovery: Product Detergent Tolerance: Equipment Needed: Format: Purify DNA from agarose gel. Pure DNA is especially well suited for sequencing and ligation reactions. Approximately 50bp to 23kb. Usually up to 5µg total DNA per column can be eluted into as little as 6µl of low salt DNA Elution Buffer or water. For DNA 50bp to 10kb, the recovery is 70-90%. For DNA 11kb to 23kb, the recovery is 50-70%. 5% Triton X-100, 5% Tween-20, 5% Sarkosyl, 0.1% SDS. Microcentrifuge, vortex. DNA PureSpin Micro Column Notes: This product is for research use only and should only be used by trained professionals. It is not intended for use in diagnostic procedures. Some reagents included with this kit are irritants. Wear protective gloves and eye protection. Follow the safety guidelines and rules enacted by your research institution or facility. Page 3

4 Page 4 PRODUCT DESCRIPTION The OmniGenX PureSpin Gel DNA Purification Kit quickly and easily purifies DNA from agarose gels. Start by adding the Agarose Dissolving Buffer to the gel slice containing your DNA sample and then transfer to the supplied DNA PureSpin Micro Column. There is no need to add any organic denaturants or chloroform. The PureSpin column technology purifies DNA in just 15 minutes and is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc. Pvull Recovered by digestomnigenx Kit Blunt-End Ligation M kb bp - Blunt-end ligation of DNA fragments purified using the OmniGenX PureSpin Gel DNA Purification Kit. Fragments from plasmid DNA digested with Pvu II restriction endonuclease were purified, then mixed and ligated for the indicated times. Effectiveness of the OmniGenX PureSpin Gel DNA Purification Kit. Lanes: M: DNA Ladder; 1-5: DNA from ladder that was excised and recovered from gel. OmniGenX gel purification kits are offered in single column format (for DNA fragments up to 23kb). The OmniGenX PureSpin Gel DNA Purification Kits are also available for large DNA (up to 200kb) gel recovery. DNA PureSpin Micro Column Page 4

5 BUFFER PREPARATION Add 24ml 100% ethanol (26ml 95% ethanol) to the 6ml DNA Wash Buffer concentrate (OD2080-WB6). Add 96ml 100% ethanol (104ml 95% ethanol) to the 24ml DNA Wash Buffer concentrate (OD2080-WB). PROTOCOLS 1. Cut and remove the DNA fragment 1 from the agarose gel using a razor blade, scalpel or other device and transfer it into a 1.5ml microcentrifuge tube. 2. Add Agarose Dissolving Buffer in a ratio of 3 : 1 to each volume of agarose excised from the gel (e.g. for 100µl (mg) of agarose gel slice add 300µl of Agarose Dissolving Buffer). 3. Proceed by incubating at C for 5-10 minutes until the gel slice is completely dis solved 2. For DNA fragments > 8kb, following the incubation step, add one additional volume of water to the mixture for better DNA recovery (e.g., 100µl agarose, 300µl Agarose Dissolving Buffer, and 100µl water). 4. Transfer the completely dissolved agarose solution to a DNA PureSpin Micro Column in a Collection Tube. 5. Centrifuge for seconds at 10,000-16,000 x g. Discard the flow-through Add 200µl of DNA Wash Buffer to the column and centrifuge for 30 seconds at 10,000-16,000 x g. Discard the flow-through. Repeat the wash step. 7. Dispense 6µl DNA Elution Buffer or water 4 directly to the column matrix. Place column into a 1.5ml tube and centrifuge for seconds at 10,000-16,000 x g to elute DNA. Ultra-pure DNA is now ready for use. Notes: 1 The amount of agarose excised from the gel should be as small as possible. 2 Do not incubate above 60 C. It is important that the gel slice dissolve completely. This can be facilitated by gentle mixing during the incubation. 3 Remove the flow-through by aspiration. Avoid contamination of the collection tube rim. 4 Elution of DNA from the column is dependent on ph and temperature. If water is used, make sure the ph is >6.0. Waiting 1 minute prior to elution may improve the yield of larger (> 6kb) DNA. For even larger DNA (> 10kb), the total yield may be improved by eluting the DNA with C DNA Elution Buffer. Page 5

6 TROUBLESHOOTING Low Recovery: Agarose is not Fully Dissolved Small globules of undissolved agarose could interfere with DNA recovery by clogging the column and leeching salts into the eluate. Gel Incubation Temperature Above 60 C DNA may be become denatured during incubation if dissolved at a higher temperature. For optimal results, dissolve the gel slice between C. Ethanol Addition Make sure ethanol has been added to the DNA Wash Buffer concentrate. Ethanol Evaporation Cap the bottle tightly to prevent evaporation over time. Inproper Addition of Elution Buffer Add DNA Elution Buffer directly to the column matrix and not to the walls of the column. Elution buffer requires contact with the matrix for at least 1 minute for large DNA 10kb. Elution Conditions DNA elution is dependent on ph, temperature, and time. For large genomic DNA ( 50kb), apply heated elution buffer (60-70 C) and incubate for several minutes prior to elution. Multiple Elutions Additional elutions may be performed for quantitatively higher recovery but lower final DNA concentration. This is recommended for DNA 10kb. Page 6

7 Low A 260 /A 230 Ratios Page 7 Contamination Be sure that the tip of the column does not come into contact with the flowthrough when removing from the collection tube. Salts from the flowthrough can contaminate a sample resulting in low A 260 / A 230 ratios. Ethanol contamination from the flowthrough can also interfere with DNA elution. OmniGenX DNA PureSpin Micro columns are designed for complete elution with no buffer retention or carryover. Supplier Q Column design causes 2-3μl CARRYOVER OmniGenX DNA PureSpin Column design ensures NO CARRYOVER Multiple Bands Appear in an Agarose Gel Use of DNA Loading Dye If water is used to elute the DNA, 6X Loading Dye containing 1mM EDTA is recommended. Most loading dyes do not contain EDTA and will acidify (ph 4) over time due to some microbial growth. This low ph is enough to cause DNA degradation. Page 7

8 ORDERING INFORMATION Product Description Catalog No. Kit Size (Preps.) PureSpin Gel DNA Purification Kit, Micro Columns w/out Caps OD2030 OD RELATED PRODUCTS For Individual Sale Catalog No. Packaging Agarose Dissolving Buffer OD2030-DB 50ml DNA Wash Buffer (concentrate) OD2080-WB 24ml DNA Elution Buffer OD2080-EB 50ml DNA PureSpin Micro Columns, Uncapped DNA PureSpin Micro Columns, Capped Collection Tubes OX1010 OX OX1030 OX OX1350 OX columns 250 columns 50 columns 250 columns 50 tubes 1000 tubes DISTRIBUTED BY E&K Scientific Products, Inc. Phone Fax Website

PureSpin DNA Clean Up Kit

PureSpin DNA Clean Up Kit PureSpin DNA Clean Up Kit Micro Columns INSTRUCTION MANUAL KIT COMPONENTS For Research Use Only PureSpin DNA Clean Up Kit, Micro Columns w/out Caps (Kit Size) OD2080 (50 Preps.) OD2080-2 (200 Preps.) Storage

More information

Zymoclean Large Fragment DNA Recovery Kit Catalog Nos. D4045 & D4046

Zymoclean Large Fragment DNA Recovery Kit Catalog Nos. D4045 & D4046 INSTRUCTION MANUAL Zymoclean Large Fragment DNA Recovery Kit Catalog Nos. D4045 & D4046 Highlights Quick (15 minute) spin column recovery of large-sized DNA (e.g., genomic, plasmid (BAC/PAC), viral, phage,

More information

Zymoclean Large Fragment DNA Recovery Kit

Zymoclean Large Fragment DNA Recovery Kit INSTRUCTION MANUAL Zymoclean Large Fragment DNA Recovery Kit Catalog Nos. D4045 & D4046 Highlights Quick (15 minute) spin column recovery of large-sized DNA (e.g., genomic, plasmid (BAC/PAC), viral, phage,

More information

Genomic DNA Clean & Concentrator -25 Catalog Nos. D4064 & D4065

Genomic DNA Clean & Concentrator -25 Catalog Nos. D4064 & D4065 INSTRUCTION MANUAL Genomic DNA Clean & Concentrator -25 Catalog Nos. D4064 & D4065 Highlights Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC),

More information

ZR-96 Genomic DNA Clean & Concentrator -5 Catalog Nos. D4066 & D4067

ZR-96 Genomic DNA Clean & Concentrator -5 Catalog Nos. D4066 & D4067 INSTRUCTION MANUAL ZR-96 Genomic DNA Clean & Concentrator -5 Catalog Nos. D4066 & D4067 Highlights 96-well plate recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage,

More information

Genomic DNA Clean & Concentrator -10 Catalog Nos. D4010 & D4011

Genomic DNA Clean & Concentrator -10 Catalog Nos. D4010 & D4011 INSTRUCTION MANUAL Genomic DNA Clean & Concentrator -10 Catalog Nos. D4010 & D4011 Highlights Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC),

More information

Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection

Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator Catalog No. D4080 Highlights Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double

More information

Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection

Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator Catalog No. D4080 Highlights Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double

More information

I. General Information 1, 2 Description Highlights Specifications Product Contents Ordering Information. II. Protocol.. 3, 4. III. List of Products 5

I. General Information 1, 2 Description Highlights Specifications Product Contents Ordering Information. II. Protocol.. 3, 4. III. List of Products 5 INSTRUCTION MANUAL ZR-96 DNA Clean-up Catalog Nos. D4017 & D4018 Contents I. General Information 1, 2 Description Highlights Specifications Product Contents Ordering Information II. Protocol.. 3, 4 III.

More information

BIO BASIC. Gel Extraction Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only

BIO BASIC. Gel Extraction Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only BIO BASIC Gel Extraction Kit 9K-006-0001s (10 preps) 9K-006-0001 (100 preps) 9K-006-0002 (200 preps) For Research Use Only Index Gel Extraction Kit Introduction 3 Important Notes 4 General Protocol 5 Troubleshooting

More information

Bead Type (NaI) Gel Extraction Kits

Bead Type (NaI) Gel Extraction Kits Bead Type (NaI) Contents Kit Contents Principle Important Notes Bead Type (NaI) Gel Extraction Kit Protocol Troubleshooting Guide 2 3 3 4 5 Ordering Information 6 Kit Contents Catalog No. Number of preparations

More information

GenepHlow Gel Extraction Kit

GenepHlow Gel Extraction Kit Instruction Manual Ver. 02.10.17 For Research Use Only GenepHlow Gel Extraction Kit DFG004 (4 Preparation Sample Kit) DFG100 (100 Preparation Kit) DFG300 (300 Preparation Kit) Advantages Convenient: includes

More information

Monarch DNA Gel Extraction Kit

Monarch DNA Gel Extraction Kit NUCLEIC ACID PURIFICATION Monarch DNA Gel Extraction Kit Instruction Manual NEB #T1020S/L Version 1.2 3/17 be INSPIRED drive DISCOVERY stay GENUINE This product is intended for research purposes only.

More information

For Research Use Only Ver

For Research Use Only Ver INSTRUCTION MANUAL Oligo Clean & Concentrator Catalog Nos. D4060 & D4061 Highlights Quick (2 minute) recovery of ultra-pure DNA and RNA oligonucleotides. Complete removal of dyes, salts, enzymes, nucleotides,

More information

Gel Extraction & PCR Purification Combo Kit (Spin-column)

Gel Extraction & PCR Purification Combo Kit (Spin-column) Note: for laboratory research use only. Gel Extraction & PCR Purification Combo Kit (Spin-column) Cat#: DP1501 (50 preps) DP1502 (100 preps) DP1503 (200 preps) BioTeke Corporation I. Kit Content Storage

More information

Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.

Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column. INSTRUCTION MANUAL ZymoPURE Plasmid Miniprep Kit Catalog Nos. D4209, D4210, D4211 & D4212 (Patent Pending) Highlights Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using

More information

Large DNA Fragments Extraction Kit

Large DNA Fragments Extraction Kit Instruction Manual Ver. 04.25.17 For Research Use Only Large DNA Fragments Extraction Kit DFL004 (4 Preparation Sample Kit) DFL100 (100 Preparation Kit) DFL300 (300 Preparation Kit) Advantages Efficient:

More information

BIO BASIC. PCR Purification Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only

BIO BASIC. PCR Purification Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only BIO BASIC PCR Purification Kit 9K-006-0003s (10 preps) 9K-006-0003 (100 preps) 9K-006-0004 (200 preps) For Research Use Only Index PCR Purification Kit Introduction 3 Important Notes 4 General Protocol

More information

Introduction. Kit components. 50 Preps GF-PC-050. Product Catalog No. 200 Preps GF-PC Preps GF-PC Preps SAMPLE

Introduction. Kit components. 50 Preps GF-PC-050. Product Catalog No. 200 Preps GF-PC Preps GF-PC Preps SAMPLE Introduction The GF-1 PCR Clean Up Kit is a system designed for rapid clean up of DNA bands ranging from 100bp to 20kb. The GF-1 PCR Clean Up Kit contains special buffers to provide the correct salt concentration

More information

Select-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085

Select-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085 INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085 Highlights Tunable: Quick 10 minute protocol for size selection can be tuned from 100 bp to 1000 bp with

More information

ZR-96 Oligo Clean & Concentrator Catalog Nos. D4062 & D4063

ZR-96 Oligo Clean & Concentrator Catalog Nos. D4062 & D4063 INSTRUCTION MANUAL ZR-96 Oligo Clean & Concentrator Catalog Nos. D4062 & D4063 Highlights Quick, high-throughput (96-well) recovery of ultra-pure DNA and RNA oligonucleotides. Complete removal of dyes,

More information

Gel/PCR Extraction Kit

Gel/PCR Extraction Kit Gel/PCR Extraction Kit Item No: EX-GP200 (200rxns) Content Content Binding Buffer BD Wash Buffer PE Elution Buffer (10 mm Tris-HCl, ph 8.5) Spin Columns EX-GP200 80 ml 20 mlx3 10 ml 200 each Description

More information

TIANgel Mini DNA Purification Kit

TIANgel Mini DNA Purification Kit TIANgel Mini DNA Purification Kit For DNA purification from agarose and polyacrylamide gels www.tiangen.com/en DP130419 TIANgel Mini DNA Purification Kit Kit Contents (Spin column) Cat. no. DP208 Contents

More information

mdi Nano Gel Extraction Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)

mdi Nano Gel Extraction Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA) Nano Gel Extraction Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax: Industrial

More information

For Research Use Only Ver

For Research Use Only Ver Page 0 INSTRUCTION MANUAL RNA Clean & Concentrator -5 Catalog Nos. R1013 & R1014 (supplied with DNase I) R1015 & R1016 Highlights Quick, 5 minute recovery of ultra pure RNA ( 17 nt) from enzymatic reactions,

More information

Soil DNA Extraction Kit

Soil DNA Extraction Kit Instruction Manual Ver. 09.23.16 For Research Use Only Soil DNA Extraction Kit Advantages IB47800 (4 Preparation Sample Kit) IB47801 (50 Preparation Kit) IB47802 (100 Preparation Kit) Sample: 250-500 mg

More information

mdi Gel Extraction Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)

mdi Gel Extraction Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA) Gel Extraction Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax: Industrial

More information

Eluted DNA is high quality and inhibitor-free making it ideal for PCR and genotyping.

Eluted DNA is high quality and inhibitor-free making it ideal for PCR and genotyping. INSTRUCTION MANUAL ZR DNA-Card Extraction Kit Catalog No. D6040 Highlights Get the DNA out! Simple and efficient procedure for the purification of DNA from samples collected onto Guthrie, FTA, and other

More information

ChIP DNA Clean & Concentrator Catalog Nos. D5201 & D5205

ChIP DNA Clean & Concentrator Catalog Nos. D5201 & D5205 Page 0 INSTRUCTION MANUAL ChIP DNA Clean & Concentrator Catalog Nos. D5201 & D5205 Highlights Quick (2 minute) recovery of ultra-pure DNA from chromatin immunoprecipitation (ChIP) assays, cell lysates,

More information

mdi Micro Gel Extraction Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)

mdi Micro Gel Extraction Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA) Micro Gel Extraction Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax: Industrial

More information

Presto Mini Plasmid Kit

Presto Mini Plasmid Kit Instruction Manual Ver. 03.06.17 For Research Use Only Presto Mini Plasmid Kit PDH004 (4 Preparation Sample Kit) PDH100 (100 Preparation Kit) PDH300 (300 Preparation Kit) Advantages Sample: 1-7 ml of cultured

More information

For Research Use Only Ver

For Research Use Only Ver INSTRUCTION MANUAL Quick-DNA FFPE Kit Catalog Nos. D3067 Highlights Streamlined purification of high-quality FFPE tissue DNA that is ideal for PCR, Next-Gen library prep, enzymatic manipulations, etc.

More information

Description...1 Components...1 Storage... 1 Technical Information...1 Protocol...2 Examples using the kit...4 Troubleshooting...

Description...1 Components...1 Storage... 1 Technical Information...1 Protocol...2 Examples using the kit...4 Troubleshooting... QuickClean II Gel Extraction Kit Cat. No. L00418 Technical Manual No. TM0594 Version: 03042011 I II III IV V VI VII VIII Description......1 Components.....1 Storage.... 1 Technical Information....1 Protocol.....2

More information

Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).

Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate). Page 0 INSTRUCTION MANUAL Quick-DNA Miniprep Kit Catalog Nos. D3024 & D3025 Highlights Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs

More information

ZR DNA Sequencing Clean-up Kit

ZR DNA Sequencing Clean-up Kit INSTRUCTION MANUAL ZR DNA Sequencing Clean-up Kit Catalog Nos. D40 & D4051 Highlights Simple 2 Minute Bind, Wash, Elute Procedure Flexible 6-20 µl Elution Volumes Allow for Direct Loading of Samples with

More information

ZR-96 DNA Clean & Concentrator-5 Catalog Nos. D4023 & D4024

ZR-96 DNA Clean & Concentrator-5 Catalog Nos. D4023 & D4024 ZYMO RESEARCH The Beauty of Science is to Make Things Simple INSTRUCTION MANUAL ZR-96 DNA Clean & Concentrator-5 Catalog Nos. D4023 & D4024 Highlights Quick (15 minute), large-scale recovery of ultra-pure

More information

Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge spin-column.

Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge spin-column. INSTRUCTION MANUAL ZymoPURE II Plasmid Maxiprep Kit Catalog Nos. D4202 & D4203 (Patent Pending) Highlights Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge

More information

Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge spin-column.

Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge spin-column. INSTRUCTION MANUAL ZymoPURE II Plasmid Maxiprep Kit Catalog Nos. D4202 & D4203 (Patent Pending) Highlights Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge

More information

Presto Mini gdna Bacteria Kit

Presto Mini gdna Bacteria Kit Instruction Manual Ver. 02.10.17 For Research Use Only Presto Mini gdna Bacteria Kit Advantages GBB004 (4 Preparation Sample Kit) GBB100/101 (100 Preparation Kit) GBB300/301 (300 Preparation Kit) Sample:

More information

Presto Food DNA Extraction Kit

Presto Food DNA Extraction Kit Instruction Manual Ver. 06.28.17 For Research Use Only Presto Food DNA Extraction Kit Advantages FGD004 (4 Preparation Sample Kit) FGD100 (100 Preparation Kit) FGD300 (300 Preparation Kit) Sample: 200

More information

TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0

TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0 Cat. # 9762 For Research Use TaKaRa MiniBEST Agarose Gel Product Manual Table of Contents I. Description... 3 II. Kit Components... 3 III. Shipping and Storage... 3 IV. Preparation before Use... 4 V. Protocol...

More information

Plus DNA Clean/Extraction Kit

Plus DNA Clean/Extraction Kit Plus DNA Clean/Extraction Kit Cat. # : DP034P/ DP034P-300 Size : 50/300 Reactions Store at RT For research use only 1 Description: The Plus DNA Clean/Extraction Kit is designed to extract DNA fragments

More information

Micro-Elute DNA Clean/Extraction Kit

Micro-Elute DNA Clean/Extraction Kit Micro-Elute DNA Clean/Extraction Kit (PCR Clean-up/Gel Extraction/DNA Clean-Up /Concentration Kit) Cat. # : DP034ME/ DP034ME-300 Size : 50/300 Reactions Store at RT For research use only 1 Description:

More information

Zymoclean Gel DNA Recovery Kit Catalog Nos. D4001T, D4001, D4002, D4007 & D4008

Zymoclean Gel DNA Recovery Kit Catalog Nos. D4001T, D4001, D4002, D4007 & D4008 INSTRUCTION MANUAL Zymoclean Gel DNA Recovery Kit Catalog Nos. D4001T, D4001, D4002, D4007 & D4008 Highlights Quick (15 minute) high-yield recovery of ultra-pure DNA from agarose gels. Column design permits

More information

DNA Visualizer Extraction Kit

DNA Visualizer Extraction Kit DNA Visualizer Extraction Kit Catalog Number D0006 50 reactions Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...

More information

Presto Stool DNA Extraction Kit

Presto Stool DNA Extraction Kit Instruction Manual Ver. 10.21.17 For Research Use Only Presto Stool DNA Extraction Kit Advantages STLD004 (4 Preparation Sample Kit) STLD050 (50 Preparation Kit) STLD100 (100 Preparation Kit) Sample: 180-200

More information

Presto Soil DNA Extraction Kit

Presto Soil DNA Extraction Kit Instruction Manual Ver. 02.23.17 For Research Use Only Presto Soil DNA Extraction Kit Advantages SLD004 (4 Preparation Sample Kit) SLD050 (50 Preparation Kit) SLD100 (100 Preparation Kit) Sample: 250-500

More information

Product # Specifications. Kit Specifications Column Binding Capacity. 10 g Maximum Weight of Gel Slice Average DNA Recovery 70 90%

Product # Specifications. Kit Specifications Column Binding Capacity. 10 g Maximum Weight of Gel Slice Average DNA Recovery 70 90% 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com DNA Gel Extraction Kit Product # 13100 Product Insert The Norgen

More information

For Research Use Only Ver

For Research Use Only Ver INSTRUCTION MANUAL Quick-RNA Fecal/Soil Microbe Microprep Kit Catalog No. R2040 Highlights Quick, 10 minute isolation of total RNA (~10 µg) from various soil and fecal samples using ultra-high density

More information

Kit for total DNA purification and isolation from agarose gels

Kit for total DNA purification and isolation from agarose gels Cat. No. EM26 Version: 1.2015 Kit for total DNA purification and isolation from agarose gels EXTRACTME is a registered trademark of BLIRT S.A. www.dnagdansk.com 2 Cat. No. EM26 I. INTENDED USE The EXTRACTME

More information

GeneJET Gel Extraction Kit #K0691, #K0692. }!tc!!!#!!h~ PA-K069

GeneJET Gel Extraction Kit #K0691, #K0692. }!tc!!!#!!h~ PA-K069 #K0691, #K0692 }!tc!!!#!!h~ PA-K069 CONTENTS page COMPONENTS OF THE KIT... 2 STORAGE AND STABILITY... 2 DESCRIPTION... 2 PRINCIPLE... 3 IMPORTANT NOTES... 3 ADDITIONAL MATERIALS AND EQUIPMENT REQUIRED...

More information

Innovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps.

Innovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps. INSTRUCTION MANUAL ZR BAC DNA Miniprep Kit Catalog Nos. D4048 & D4049 Highlights For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, endonuclease digestion,

More information

Innovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps.

Innovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps. INSTRUCTION MANUAL ZR BAC DNA Miniprep Kit Catalog Nos. D4048 & D4049 Highlights For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, endonuclease digestion,

More information

Kit for total DNA isolation from agarose gels

Kit for total DNA isolation from agarose gels Cat. No. EM08 Version: 1.2013 Kit for total DNA isolation from agarose gels EXTRACTME is a registered trademark of BLIRT S.A. www.dnagdansk.com Cat. No. EM08 I. INTENDED USE The EXTRACTME DNA GEL-OUT kit

More information

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions

More information

The fastest, easiest, most reliable method for purification of up to 1.2 mg of ultra-pure endotoxin-free plasmid DNA.

The fastest, easiest, most reliable method for purification of up to 1.2 mg of ultra-pure endotoxin-free plasmid DNA. INSTRUCTION MANUAL ZymoPURE - Express Plasmid Midiprep Kit Catalog No. D4213 (Patent Pending) Highlights Innovative ZymoPURE - Express pellet-free procedure bypasses the standard cell-pelleting and resuspension

More information

INDEX KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 BENEFITS

INDEX KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 BENEFITS INDEX KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 BENEFITS ERRORE. IL SEGNALIBRO NON È DEFINITO. IMPORTANT NOTES 1 METHOD FOR DNA EXTRACTION FROM AGAROSE GELS 2 METHOD FOR DNA EXTRACTION FROM

More information

Pinpoint Slide RNA Isolation System I Catalog No. R1003

Pinpoint Slide RNA Isolation System I Catalog No. R1003 INSTRUCTION MANUAL Pinpoint Slide RNA Isolation System I Catalog No. R1003 Highlights Allows for the isolation of total RNA from tissue mounted on glass slides. Simple procedure combines Pinpoint tissue

More information

Pinpoint Slide RNA Isolation System I Catalog No. R1003

Pinpoint Slide RNA Isolation System I Catalog No. R1003 INSTRUCTION MANUAL Pinpoint Slide RNA Isolation System I Catalog No. R003 Highlights Allows for the isolation of total RNA from tissue mounted on glass slides. Simple procedure combines Pinpoint tissue

More information

The fastest, simplest method for purifying the highest quality endonuclease-free plasmid DNA.

The fastest, simplest method for purifying the highest quality endonuclease-free plasmid DNA. INSTRUCTION MANUAL Zyppy Plasmid Midiprep Kit Catalog Nos. D4025 & D4026 (Patent Pending) Highlights The fastest, simplest method for purifying the highest quality endonuclease-free plasmid DNA. Pellet-Free

More information

INSTRUCTION MANUAL. ZR Fecal DNA Kit Catalog No. D6010. Highlights. Contents

INSTRUCTION MANUAL. ZR Fecal DNA Kit Catalog No. D6010. Highlights. Contents INSTRUCTION MANUAL ZR Fecal DNA Kit Catalog No. D6010 Highlights Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in as little as 15 minutes including humans, birds,

More information

ZR-96 DNA Sequencing Clean-up Kit Catalog Nos. D4052 & D4053

ZR-96 DNA Sequencing Clean-up Kit Catalog Nos. D4052 & D4053 INSTRUCTION MANUAL ZR-96 DNA Sequencing Clean-up Kit Catalog Nos. D4052 & D4053 Highlights Simple 10 Minute Bind, Wash, Elute Procedure Flexible 15-20 µl Elution Volumes Allow for Direct Loading of Samples

More information

User Bulletin. Shipping & Storage. Gel/PCR DNA Isolation System. Protocol. WARNING: strong acids and oxidants (such as bleach) should not

User Bulletin. Shipping & Storage. Gel/PCR DNA Isolation System. Protocol. WARNING: strong acids and oxidants (such as bleach) should not User Bulletin VER.:17A Shipping & Storage Viogene Gel/PCR DNA Isolation System is shipped and storage at ambient temperature up to 12 months. Gel/PCR DNA Isolation System Purification of small-scale DNA

More information

Quick-DNA Fungal/Bacterial Microprep Kit Catalog No. D6007

Quick-DNA Fungal/Bacterial Microprep Kit Catalog No. D6007 INSTRUCTION MANUAL Quick-DNA Fungal/Bacterial Microprep Kit Catalog No. D6007 Highlights Simple, efficient isolation of DNA (up to 5 µg/prep) from all types of tough-to-lyse fungi (e.g., yeast) and bacteria

More information

For Research Use Only Ver

For Research Use Only Ver INSTRUCTION MANUAL Quick-DNA/RNA FFPE Kit Catalog No. R1009 Highlights High performance sample prep technology for total nucleic acid (DNA & RNA) recovery from the same FFPE tissue sample/section. High

More information

Gel Extraction Mini Spin Column Kit. UltraPrep Gel-Ex. Purification of DNA fragments and plasmids from agarose gels

Gel Extraction Mini Spin Column Kit. UltraPrep Gel-Ex. Purification of DNA fragments and plasmids from agarose gels Gel Extraction Mini Spin Column Kit UltraPrep Gel-Ex Purification of DNA fragments and plasmids from agarose gels 2006 Molzym, all rights reserved 1 UltraPrep Gel-Ex Manual 01/2006 Contents Kit contents

More information

Gene-Spin DNA Purification Kit - V3

Gene-Spin DNA Purification Kit - V3 Protech Technology 波仕特生物科技 Gene-Spin 1-4-3 DNA Purification Kit - V3 (For PCR/DNA clean-up & Gel Extraction) Innovative Tools for Nucleic Acid Purification This Kit is for research purposes only. Not for

More information

Size-Select Kit for NGS Library Preparation Product # 53600

Size-Select Kit for NGS Library Preparation Product # 53600 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Size-Select Kit for NGS Library Preparation Product # 53600 Product

More information

E.Z.N.A. Gel Extraction Kit

E.Z.N.A. Gel Extraction Kit E.Z.N.A. Gel Extraction Kit D2500-00 5 preps D2500-01 50 preps D2500-02 200 preps D2501-00 5 preps D2501-01 50 preps D2501-02 200 preps Manual Date: November 2018 Revision Number: v3.1 E.Z.N.A. Gel Extraction

More information

Bacteria Genomic DNA Purification Kit

Bacteria Genomic DNA Purification Kit Bacteria Genomic DNA Purification Kit Cat #:DP025/ DP025-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Bacteria Genomic DNA Purification Kit provides a rapid, simple, and

More information

PureLink Quick Gel Extraction Kit

PureLink Quick Gel Extraction Kit USER GUIDE PureLink Quick Gel Extraction Kit For purifying DNA fragments from agarose gels Catalog Numbers K2100-12, K2100-25 Revision Date 21 March 2011 Publication Part Number 25-0790 MAN0000487 ii Contents

More information

TIANquick Mini Purification Kit

TIANquick Mini Purification Kit TIANquick Mini Purification Kit For purification of PCR products, 100 bp to 10 kb www.tiangen.com/en DP121221 TIANquick Mini Purification Kit (Spin column) Cat no. DP203 Kit Contents Contents Buffer BL

More information

Plasmid DNA Extraction Miniprep Kit

Plasmid DNA Extraction Miniprep Kit BIO BASIC Plasmid DNA Extraction Miniprep Kit 9K-006-0009s (10 preps) 9K-006-0009 (100 preps) 9K-006-0010 (200 preps) For Research Use Only Index Plasmid DNA Extraction Miniprep Kit Introduction 3 Important

More information

Viral Nucleic Acid Isolation

Viral Nucleic Acid Isolation H A N D B O O K Genetix Biotech Asia Pvt. Ltd. 71/1, First Floor, Shivaji Marg, Najafgarh Road, New Delhi - 110015 Phone : +91-11-45027000 n Fax : +91-11-25419631 E-mail : info@genetixbiotech.com n www.genetixbiotech.com

More information

Quick-DNA Tissue/Insect Microprep Kit Catalog No. D6015

Quick-DNA Tissue/Insect Microprep Kit Catalog No. D6015 INSTRUCTION MANUAL Quick-DNA Tissue/Insect Microprep Kit Catalog No. D6015 Highlights Simple method for the isolation of DNA (up to 5 µg) from fresh, frozen, or stored insect and arthropod specimens in

More information

Innovative centrifugation based procedure omits conventional cell pelleting and re-suspension steps.

Innovative centrifugation based procedure omits conventional cell pelleting and re-suspension steps. INSTRUCTION MANUAL Zyppy -96 Plasmid Miniprep Catalog Nos. D4041, D4042, & D4043 Highlights Innovative centrifugation based procedure omits conventional cell pelleting and re-suspension steps. The fastest

More information

Strep-Spin Protein Miniprep Kit Catalog No. P2004, P2005

Strep-Spin Protein Miniprep Kit Catalog No. P2004, P2005 INSTRUCTION MANUAL Strep-Spin Protein Miniprep Kit Catalog No. P2004, P2005 Highlights Fast protocol to purify Strep-tagged proteins from cell-free extracts Screen your recombinant colonies directly for

More information

State-of-the-art, ultra-high density BashingBeads are fracture resistant and chemically inert.

State-of-the-art, ultra-high density BashingBeads are fracture resistant and chemically inert. INSTRUCTION MANUAL ZR Fecal DNA MiniPrep Catalog No. D6010 Highlights Rapid method for the isolation of inhibitor-free, PCR-quality DNA (up to 25 µg/prep) from fecal samples in as little as 15 minutes

More information

Kit for total DNA isolation from agarose gels

Kit for total DNA isolation from agarose gels Cat. No. EM08.1 Version: 1.2019 NEW VERSION Kit for total DNA isolation from agarose gels EXTRACTME is a registered trademark of BLIRT S.A. www.blirt.eu 2 Cat. No. EM08.1 I. INTENDED USE The EXTRACTME

More information

Product # Specifications. Kit Specifications Column Binding Capacity 50 µg Maximum Column Loading Volume 700 µl

Product # Specifications. Kit Specifications Column Binding Capacity 50 µg Maximum Column Loading Volume 700 µl 344 Merritt Street St. Catharines, ON, Canada L2T 1K6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Leukocyte RNA Purification Kit Product # 21200 Product Insert

More information

Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).

Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate). INSTRUCTION MANUAL Quick-DNA Microprep Kit Catalog Nos. D3020 & D3021 Highlights Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs or cultured

More information

ReliaPrep DNA Clean-Up and Concentration System

ReliaPrep DNA Clean-Up and Concentration System TECHNICAL MANUAL ReliaPrep DNA Clean-Up and Concentration System Instructions for Use of Products A2891, A2892 and A2893 6/18 TM540 ReliaPrep DNA Clean-Up and Concentration System All technical literature

More information

For Research Use Only Ver

For Research Use Only Ver INSTRUCTION MANUAL ZymoBIOMICS DNA/RNA Mini Kit Catalog No. R2002 Highlights Rapid, robust, and simple purification of high quality, inhibitor-free DNA and total RNA (including small/micro RNAs) from any

More information

Strep-Spin Protein Miniprep Kit Catalog No. P2004 & P2005

Strep-Spin Protein Miniprep Kit Catalog No. P2004 & P2005 INSTRUCTION MANUAL Strep-Spin Protein Miniprep Kit Catalog No. P2004 & P2005 Highlights Fast & Simple: Purify Strep-tagged proteins from cell-free extracts using a spin-column in 7 minutes High-Quality:

More information

Zymo-Spin Technology. The microcentrifuge column that revolutionized nucleic acid clean-up

Zymo-Spin Technology. The microcentrifuge column that revolutionized nucleic acid clean-up Zymo-Spin Technology The microcentrifuge column that revolutionized nucleic acid clean-up Overview A Comprehensive Solution for DNA and RNA Clean-Up High-quality, inhibitor-free DNA and RNA is crucial

More information

Quick-DNA Fungal/Bacterial Miniprep Kit Catalog No. D6005

Quick-DNA Fungal/Bacterial Miniprep Kit Catalog No. D6005 INSTRUCTION MANUAL Quick-DNA Fungal/Bacterial Miniprep Kit Catalog No. D6005 Highlights Simple, efficient isolation of DNA (up to 25 µg/prep) from all types of tough-to-lyse fungi (e.g., yeast) and bacteria

More information

Presto 96 Well gdna Bacteria Kit

Presto 96 Well gdna Bacteria Kit Presto 96 Well gdna Bacteria Kit 96GBB02 (2 x 96 well plates/kit) 96GBB04 (4 x 96 well plates/kit) 96GBB10 (10 x 96 well plates/kit) Instruction Manual Ver. 05.04.17 For Research Use Only Advantages Sample:

More information

Chromatrap 96 DNA Micro Elution Purification Kit

Chromatrap 96 DNA Micro Elution Purification Kit V 1.0 Chromatrap 96 DNA Micro Elution Purification Kit High throughput plates for ultra-pure DNA Protocol v1.0 Catalogue no. 500240 ADVANCEMENTS IN EPIGENETICS Contents Kit Components and Storage 3 Additional

More information

BioTeke Corporation. Endotoxin-free Plasmid DNA Mini-preparation Kit. (Spin-column) Note: for laboratory research use only.

BioTeke Corporation. Endotoxin-free Plasmid DNA Mini-preparation Kit. (Spin-column) Note: for laboratory research use only. Note: for laboratory research use only. Endotoxin-free Plasmid DNA Mini-preparation Kit (Spin-column) Cat. # DP2601 (20 preps) DP2602 (50 preps) BioTeke Corporation 1 I. Kit Content Storage and Stability

More information

E.Z.N.A. PF Micro RNA Kit. R preps R preps

E.Z.N.A. PF Micro RNA Kit. R preps R preps E.Z.N.A. PF Micro RNA Kit R7036-00 5 preps R7036-01 50 preps June 2013 E.Z.N.A. PF Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

StrataPrep PCR Purification Kit

StrataPrep PCR Purification Kit StrataPrep PCR Purification Kit INSTRUCTION MANUAL Catalog #400771 and #400773 Revision A For In Vitro Use Only 400771-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this

More information

Quick-DNA Tissue/Insect Miniprep Kit Catalog No. D6016

Quick-DNA Tissue/Insect Miniprep Kit Catalog No. D6016 INSTRUCTION MANUAL Quick-DNA Tissue/Insect Miniprep Kit Catalog No. D6016 Highlights Simple method for the isolation of DNA (up to 25 µg) from fresh, frozen, or stored insect and arthropod specimens in

More information

For Research Use Only Ver

For Research Use Only Ver INSTRUCTION MANUAL ZymoBIOMICS RNA Mini Kit Catalog No. R2001 Highlights Rapid, robust, and simple purification of high quality, inhibitor-free total RNA (including small/micro RNAs) from any sample including

More information

ITS Sequencing in Millepora. 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector

ITS Sequencing in Millepora. 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector Page 1 of 5 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector 1. Digest 1 µg of pbluescript with Eco RI 2. Following digestion, add 0.1 volumes of 3M sodium acetate (ph

More information

For Research Use Only Ver

For Research Use Only Ver INSTRUCTION MANUAL Quick-RNA Whole Blood Catalog Nos. R1201 Highlights Purify high-quality total RNA (including small/micro RNAs) from whole and partitioned blood samples. Compatible with commonly used

More information

Stool DNA Isolation Kit

Stool DNA Isolation Kit Product Information Norgen s provides a convenient and rapid method to isolate total DNA from fresh or frozen stool samples. The universal protocol conveniently allows for the isolation of total genomic

More information

E.Z.N.A. mirna Kit. R preps R preps R preps

E.Z.N.A. mirna Kit. R preps R preps R preps E.Z.N.A. mirna Kit R7034-00 5 preps R7034-01 50 preps R7034-02 200 preps August 2011 E.Z.N.A. Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Quick-DNA Plant/Seed Miniprep Kit Catalog No. D6020

Quick-DNA Plant/Seed Miniprep Kit Catalog No. D6020 INSTRUCTION MANUAL Quick-DNA Plant/Seed Miniprep Kit Catalog No. D6020 Highlights Simple method for the isolation of inhibitor-free, PCR-quality DNA (up to 25 µg/prep) from a variety of plant and seed

More information

E.Z.N.A. FFPE RNA Kit. R preps R preps R preps

E.Z.N.A. FFPE RNA Kit. R preps R preps R preps E.Z.N.A. FFPE RNA Kit R6954-00 5 preps R6954-01 50 preps R6954-02 200 preps January 2015 E.Z.N.A. FFPE RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Kit Specifications 45 g. 45 g of RNA 8 L

Kit Specifications 45 g. 45 g of RNA 8 L RNA Clean-Up and Concentration Micro-Elute Kit Product # 61000 Product Insert Norgen s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration

More information