Title: Description of the First Escherichia coli Clinical Isolate Harboring Colistin-
|
|
- Emily Abigayle Paul
- 6 years ago
- Views:
Transcription
1 AAC Accepted Manuscript Posted Online 24 October 2016 Antimicrob. Agents Chemother. doi: /aac Copyright 2016, American Society for Microbiology. All Rights Reserved. 1 2 Title: Description of the First Escherichia coli Clinical Isolate Harboring Colistin- Resistance mcr-1 Gene from the Indian Subcontinent Mashkoor Mohsin 1#, Shahbaz Raza 1, Nicole Roschanski 2, Sebastian Guenther 2, Aamir Ali 3,4, Peter Schierack Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan Institute of Animal Hygiene and Environmental Health, Freie Universitaet Berlin, Berlin, Germany Faculty of Environment and Natural Sciences, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany 12 4 National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan Running title: Colistin resistance from human in the Indian Subcontinent Keywords: Polymyxins, mcr-1, ESBL, CTX-M-15, IncI2, Indian Subcontinent # Corresponding Author: Mashkoor Mohsin, Institute of Microbiology, University of Agriculture, Faisalabad 38000, Pakistan. Tel: mashkoormohsin@uaf.edu.pk 1
2 The first report of plasmid-mediated colistin resistance among Enterobacteriaceae in China sounded alarms in the medical community worldwide (1). Since, E. coli isolates harboring colistin resistance encoded by the mcr-1 gene have been globally reported (2). To our knowledge, we hereby report the first detection of mcr-1 gene in a human E. coli isolate from the Indian subcontinent In February 2016, a total of 29 extended-spectrum beta-lactamase (ESBL)-producing E. coli isolates recovered from patients related to wound infections admitted to Burn & Reconstructive Surgery Centre, Allied Hospital Faisalabad, Pakistan were tested for plasmidmediated colistin resistance. Phenotypic resistance to polymyxin B was determined by vitek-2 compact system (biomérieux, Germany) followed by determination of the colistin MIC by broth microdilution in 96-well plate test panel (Micronaut S, Merlin, Bornheim-Hersel, Germany). One colistin resistant E. coli isolate (X26) with MIC of 4 μg/ml was found from a 35-year old male patient suffering from burn-associated wounds. Presence of the mcr-1 gene was confirmed by TaqMan-based real-time PCR (3). The isolate exhibited multidrug resistance, but was susceptible to carbapenems (Table 1). The presence of the bla CTX-M-15 gene in E. coli X26 was confirmed by PCR and sequencing (4) The plasmid-carriage of E. coli isolate X26 was analyzed using a conventional plasmid profile analysis (5). The conjugative transfer ability of the colistin resistance was investigated by plasmid conjugation experiments using sodium azide resistant E. coli K12- J53 as a recipient strain. Transconjugants were selected on LB agar plates supplemented with colistin (4 μg/ml) and sodium azide (100 μg/ml) and subsequently confirmed by PCR for the mcr-1 gene. PCRbased plasmid replicon typing of transconjugants showed IncI2 type. PFGE was performed to determine genetic relatedness of the E. coli X26 with the recently reported mcr-1 carrying ESBL-E. coli isolate PK-13 of ST354 recovered from wild migratory bird in Pakistan (6). Multi-locus sequence typing (MLST) was carried out using the method developed by Wirth et 2
3 47 al. (7). MLST results revealed E. coli X26 as a new ST Colistin has become the last line of defense for the treatment of infections caused by Gramnegative bacteria, in particular carbapenem-resistant Enterobacteriaceae (CRE). The presence of plasmid-mediated colistin resistance in Indian subcontinent where there is already burden of CRE, is worrisome as it threatened the use of last resort antibiotics. Recently, we have reported the mcr-1 gene in E. coli isolated from a wild migratory bird in Pakistan (6). The plasmid size (~ 63 kb) as well as the replicon type of E. coli X26 correspond to that described by Liu and colleagues (1) and our recent findings (6). Thus, indicating that IncI2 represent a major plasmid type involved in the spread of colistin resistance in our region. The PFGE pattern and ST type showed no genetic relatedness between E. coli X26 and our recently reported strain (6), suggesting that different clonal types are involved in the dissemination of mcr-1 gene. In conclusion, the presence of the plasmid-mediated colistin resistance in a clinical isolate, adds to the worry as this potent gene may spread to other susceptible bacteria, making pan-resistant pathogens Acknowledgements: Mashkoor Mohsin was supported by postdoctoral fellowship from the Alexander von Humboldt Foundation, Germany. 64 Funding Infromation 65 This study received no specific funding
4 69 References Liu Y-Y, Wang Y, Walsh TR, Yi L-X, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu L-F, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu J- H, Shen J Emergence of plasmid-mediated colistin resistance mechanism MCR- 1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 16: Castanheira M, Griffin MA, Deshpande LM, Mendes RE, Jones RN, Flamm RK Detection of mcr-1 among Escherichia coli Clinical Isolates Collected Worldwide as Part of the SENTRY Antimicrobial Surveillance Program in 2014 and Antimicrob Agents Chemother 60: Irrgang A, Roschanski N, Tenhagen B-A, Grobbel M, Skladnikiewicz-Ziemer T, Thomas K, Roesler U, Kasbohrer A Prevalence of mcr-1 in E. coli from Livestock and Food in Germany, PLoS One 11:e Ewers C, Grobbel M, Stamm I, Kopp PA, Diehl I, Semmler T, Fruth A, Beutlich J, Guerra B, Wieler LH, Guenther S Emergence of human pandemic O25:H4- ST131 CTX-M-15 extended-spectrum-beta-lactamase-producing Escherichia coli among companion animals. J Antimicrob Chemother 65: Schaufler K, Wieler LH, Semmler T, Ewers C, Guenther S ESBL-plasmids carrying toxin-antitoxin systems can be cured of wild-type Escherichia coli using a heat technique. Gut Pathog 5: Mohsin M, Raza S, Roschanski N, Schaufler K, Guenther S First description of plasmid-mediated colistin-resistant extended-spectrum β-lactamase-producing Escherichia coli in a wild migratory bird from Asia. Int J Antimicrob Agents 48:
5 Wirth T, Falush D, Lan R, Colles F, Mensa P, Wieler LH, Karch H, Reeves PR, Maiden MC, Ochman H, Achtman M Sex and virulence in Escherichia coli: an evolutionary perspective. Mol. Microbiol 60:
6 114 Table 1: Antibiotic resistance profile of E. coli X Antimicrobial Agents MIC (μg/ml) 1 Amikacin 4 Aztreonam >64 Ciprofloxacin >4 Colistin 4 Cefepime >64 Ceftazidime >64 Cefotaxime >64 Fosfomycin 16 Gentamicin >16 Imipenem 0.25 Meropenem 0.25 Marbofloxacin >4 Piperacillin/Tazobactam >128 Tetracyclin 4 Tigecycline 5 6
7 Tobramycin >16 Trimethoprim/sulfamethoxazole > MIC s were determined using the Vitek-2 system (biomérieux) with except for colistin, which was performed using broth microdilution in 96-well plate test panel (Micronaut S, Merlin, Bornheim-Hersel, Germany)
Antimicrobial Agents and Chemotherapy New Data Letter
AAC Accepted Manuscript Posted Online 15 August 2016 Antimicrob. Agents Chemother. doi:10.1128/aac.01519-16 Copyright 2016, American Society for Microbiology. All Rights Reserved. 1 Antimicrobial Agents
More informationGenome analysis of the carbapenem- and colistin-resistant Escherichia coli isolate NRZ14408
AAC Accepted Manuscript Posted Online 17 January 2017 Antimicrob. Agents Chemother. doi:10.1128/aac.02359-16 Copyright 2017 American Society for Microbiology. All Rights Reserved. 1 2 Genome analysis of
More informationSUMMARY. Key words: antibioticresistance, Enterobacteriaceae, ESBL, CTX-M,
SUMMARY Key words: antibioticresistance, Enterobacteriaceae, ESBL, CTX-M, The doctoral thesis entitled Prevalence of Enterobacteriaceae producing extendedspectrum beta-lactamases (ESBL) isolated from broilers
More informationEcology of bla NDM and mcr-1
Editorial Page 1 of 5 Ecology of bla NDM and mcr-1 Jose Francisco Delgado-Blas, Bosco R. Matamoros, Bruno Gonzalez-Zorn Department of Animal Health and VISAVET, Universidad Complutense de Madrid, Madrid,
More informationSupplementary appendix
Supplementary appendix This appendix formed part of the original submission and has been peer reviewed. We post it as supplied by the authors. Supplement to: Quan J, Li X, Chen Y, et al. Prevalence of
More informationDetection and characterization of extended spectrum β-lactamase producing Escherichia coli from poultry of eastern India
Detection and characterization of extended spectrum β-lactamase producing Escherichia coli from poultry of eastern India Dr. Samiran Bandyopadhyay Scientist Indian Veterinary Research Institute Eastern
More informationUse of Molecular Assays for Resistance Detection
Use of Molecular Assays for Resistance Detection Antimicrobial resistance and susceptibility are complex, and current in vitro methods have been developed to predict a microorganism s response to antibacterial
More informationWhen Carbapenem-Hydrolyzing ß-Lactamase KPC. attributed to outer-membrane protein deficiency coupled with plasmid-mediated
AAC Accepts, published online ahead of print on 18 July 2011 Antimicrob. Agents Chemother. doi:10.1128/aac.00719-11 Copyright 2011, American Society for Microbiology and/or the Listed Authors/Institutions.
More informationDissemination of transposon Tn6001 in carbapenem-non-susceptible and extensively drug-resistant Pseudomonas aeruginosa in Taiwan
Journal of Antimicrobial Chemotherapy (2009) 64, 1170 1174 doi:10.1093/jac/dkp341 Advance Access publication 22 September 2009 Dissemination of transposon Tn6001 in carbapenem-non-susceptible and extensively
More informationPrevalence and molecular characterization of clinical isolates of Escherichia coli expressing an AmpC phenotype
J Antimicrob Chemother 2010; 65: 460 464 doi:10.1093/jac/dkp484 Advance publication 22 January 2010 Prevalence and molecular characterization of clinical isolates of Escherichia coli expressing an AmpC
More informationOngoing epidemic of bla VIM-1 -positive Klebsiella pneumoniae in Athens, Greece: a prospective survey
Journal of Antimicrobial Chemotherapy (2008) 61, 59 63 doi:10.1093/jac/dkm443 Advance Access publication 13 November 2007 Ongoing epidemic of bla VIM-1 -positive Klebsiella pneumoniae in Athens, Greece:
More informationCuring antibiotic resistance in vivo. Muhammad Kamruzzaman
Curing antibiotic resistance in vivo Muhammad Kamruzzaman Occurrence of resistance -Some bacteria are naturally resistant to certain antibiotics -Gene mutation -Horizontal transfer of antibiotic resistance
More informationColistin resistance Lyngby, DK, April 4, Dik Mevius, Wageningen Bioveterinary Research (CVI- Lelystad)
Colistin resistance Lyngby, DK, April 4, 2018 Dik Mevius, Wageningen Bioveterinary Research (CVI- Lelystad) 2 Discovery of a gene encoding for plasmid mediated colistin resistance MCR-history Retrospective
More informationCurriculum Vitae. Abbas Maleki, Ph.D. Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
Curriculum Vitae Abbas Maleki, Ph.D Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran E-mail: abbasmaleki_ilam@yahoo.com maleki-a@medilam.ac.ir Tel: +989187419401 Personal
More informationResistance, Yonsei University College of Medicine, Seoul, Korea; and 2 Department of
AAC Accepts, published online ahead of print on March 0 Antimicrob. Agents Chemother. doi:./aac.0- Copyright 0, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
More informationOccurrence of Plasmid- and Chromosome-encoded mcr-1 in. Water-borne Enterobacteriaceae in China. Running head: Occurrence of mcr-1 in water bacteria
AAC Accepted Manuscript Posted Online 30 May 2017 Antimicrob. Agents Chemother. doi:10.1128/aac.00017-17 Copyright 2017 American Society for Microbiology. All Rights Reserved. 1 2 Occurrence of Plasmid-
More informationDetection of aac(6 0 )-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from Tel Aviv, Israel
Journal of Antimicrobial Chemotherapy (2009) 64, 718 722 doi:10.1093/jac/dkp272 Advance Access publication 4 August 2009 Detection of aac(6 0 )-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from
More informationSupplementary webappendix
Supplementary webappendix This webappendix formed part of the original submission and has been peer reviewed. We post it as supplied by the authors. Supplement to: Falgenhauer L, Waezsada S-E, Yao Y, et
More informationThe Antimicrobial Resistance Laboratory Network (ARLN) Providing CRE Colonization Testing to a State Lab in a Neighboring Region June 4, 2018
The Antimicrobial Resistance Laboratory Network (ARLN) Providing CRE Colonization Testing to a State Lab in a Neighboring Region June 4, 2018 Kimberlee Musser, PhD Chief of Bacterial Disease Northeast
More informationCharacterization of a novel metallo-β-lactamase variant, GIM-2, from a clinical isolate
AAC Accepts, published online ahead of print on 29 December 2014 Antimicrob. Agents Chemother. doi:10.1128/aac.05062-14 Copyright 2014, American Society for Microbiology. All Rights Reserved. 1 2 Characterization
More informationEmergence and persistence of integron structures harbouring VIM genes in the Children s Memorial Health Institute, Warsaw, Poland,
Journal of Antimicrobial Chemotherapy (2009) 63, 269 273 doi:10.1093/jac/dkn512 Advance Access publication 18 December 2008 Emergence and persistence of integron structures harbouring VIM genes in the
More informationCarbapenem-resistant Enterobacteriaceae (CRE): Surveillance and Response. David Selvage, MHS, PA-C New Mexico Department of Health March 15, 2016
Carbapenem-resistant Enterobacteriaceae (CRE): Surveillance and Response David Selvage, MHS, PA-C New Mexico Department of Health March 15, 2016 What are Carbapenem-resistant Enterobacteriaceae (CRE)?
More informationSupplementary Information for
Supplementary Information for Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis J. Choi 1, H. Y. Jeong 2,3, G. Y. Lee 2,4, S. Han 1, S.
More informationPrevalence and Molecular Characteristics of Carbapenemase-Producing Enterobacteriaceae From Five Hospitals in Korea
Original Article Clinical Microbiology Ann Lab Med 6;6:9- http://dx.doi.org/.4/alm.6.6.6.9 ISSN 4-86 eissn 4-84 Prevalence and Molecular Characteristics of Carbapenemase-Producing Enterobacteriaceae From
More informationCRE Laboratory Testing and CRE Lab Testing Recommendations in-depth recommendations on CRE laboratory detection
December 2014 Dear Laboratory Director, The Illinois Department of Public Health (IDPH) amended the Control of Communicable Diseases Code (77 Ill. Adm. Code 690) to require reporting of Carbapenem-Resistant
More informationBiosurveillance in a Global Age: Harnessing the Expertise of the Broad Scientific Community
UNCLASSIFIED//APPROVED FOR PUBLIC RELEASE Biosurveillance in a Global Age: Harnessing the Expertise of the Broad Scientific Community NDIA Biosurveillance Conference Dr. C. Nicole Rosenzweig US Army, Edgewood
More informationThe Prevalence of TEM-1 gene causing resistance to beta-lactam antibiotics in Klebsiella pneumoniae isolates from clinical samples and plasmid curing
Available online at www.ijmrhs.com ISSN No: 2319-5886 International Journal of Medical Research & Health Sciences, 2016, 5, 11:557-561 The Prevalence of TEM-1 gene causing resistance to beta-lactam antibiotics
More informationAAC Accepts, published online ahead of print on 2 June 2008 Antimicrob. Agents Chemother. doi: /aac
AAC Accepts, published online ahead of print on June 00 Antimicrob. Agents Chemother. doi:.11/aac.00175-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More information*Kotgire Santosh A and Tankhiwale Nilima. Sciences University Sawangi(M) Wardha, Maharashtra, India *Author for Correspondence
PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY PROFILE OF METALLO-β-LACTAMASES (M-β-L) PRODUCING PSEUDOMONAS AREUGINOSA FROM RURAL HOSPITAL: COMPARISON OF TWO DISK DIFFUSION METHODS *Kotgire Santosh A and
More informationOccurrence and Detection of AmpC β-lactamases among Enterobacteriaceae in a Tertiary Care Centre in Trivandrum, India
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 08 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.708.023
More informationH. Wu, B.-G. Liu, J.-H. Liu, Y.-S. Pan, L. Yuan and G.-Z. Hu
Phenotypic and molecular characterization of CTX-M-14 extended-spectrum β-lactamase and plasmid-mediated ACT-like AmpC β-lactamase produced by Klebsiella pneumoniae isolates from chickens in Henan Province,
More informationEmergence of pan-resistance in KPC-2 carbapenemase-producing Klebsiella pneumoniae in Crete, Greece: a close call
J Antimicrob Chemother 2016; 71: 1207 1212 doi:10.109/jac/dkv467 Advance Access publication 26 January 2016 Emergence of pan-resistance in KPC-2 carbapenemase-producing Klebsiella pneumoniae in Crete,
More informationEmergence of Ceftolozane-Tazobactam Resistant Pseudomonas aeruginosa During Treatment
AAC Accepted Manuscript Posted Online 25 September 2017 Antimicrob. Agents Chemother. doi:10.1128/aac.01183-17 Copyright 2017 American Society for Microbiology. All Rights Reserved. 1 2 Version Date: 09/20/2017
More informationANTIMICROBIAL SUSCEPTIBILITY INCREASING COMPLEXITY FOR TREATMENT OF URINARY
TRACT INFECTIONS This specimen was stated to be "from a urine culture" and was to be identified to the species level and tested for antimicrobial susceptibility. The culture contained an Escherichia coli
More informationOriginal Research article Prevalence of Metallo-betalactamases (MBL) producing Pseudomonas aeruginosa in a Tertiary care Hospital.
Original Research article Prevalence of Metallo-betalactamases (MBL) producing Pseudomonas aeruginosa in a Tertiary care Hospital. Dr Anil Rajput*, Dr Bhavin Prajapati **, Dr.Bimal Chauhan***, Dr Atit
More informationACCEPTED. Laboratory Medicine, Kosin University College of Medicine, , 34 Amnam-Dong,
AAC Accepts, published online ahead of print on 21 May 2007 Antimicrob. Agents Chemother. doi:10.1128/aac.00279-07 Copyright 2007, American Society for Microbiology and/or the Listed Authors/Institutions.
More informationESBLs and KPCs: Impact of Revised CLSI Breakpoints on testing and Reporting Algorithms
ESBLs and KPCs: Impact of Revised CLSI Breakpoints on testing and Reporting Algorithms Stephen G. Jenkins, Ph.D. Director, Clinical Microbiology Laboratories New York/Presbyterian Hospital Weill Cornell
More informationMolecular susceptibility testing
Molecular susceptibility testing Dr Andrew Ginn Supervising Scientist Antimicrobial Resistance Reference Laboratory ICPMR, Westmead Hospital Resistance genes Gram negatives Transmissible; e.g. ESBLs, MBLs,
More informationWELCOME. to the CDS WORKSHOP
WELCOME to the CDS WORKSHOP Sydney 2010 Excel Spreadsheet for Registration Recent Additions to the CDS Doripenem 10mg disc A carbapenem claimed to be more active against Pseudomonas than Meropenem Daptomycin:
More informationOriginal Article Detection of integrons in Escherichia coli producing plasmid-mediated AmpC β-lactamases
Int J Clin Exp Med 2019;12(2):1690-1696 www.ijcem.com /ISSN:1940-5901/IJCEM0079457 Original Article Detection of integrons in Escherichia coli producing plasmid-mediated AmpC β-lactamases Juanjuan Ding,
More informationDepartment of Microbiology, University College of Medical Sciences & Guru Tegh Bahadur Hospital & *
Indian J Med Res 122, October 2005, pp 330-337 Phenotypic characteristics of clinical isolates of Klebsiella pneumoniae & evaluation of available phenotypic techniques for detection of extended spectrum
More informationImipenem-susceptible, meropenem-resistant Klebsiella pneumoniae producing
AAC Accepts, published online ahead of print on 15 December 2014 Antimicrob. Agents Chemother. doi:10.1128/aac.04330-14 Copyright 2014, American Society for Microbiology. All Rights Reserved. 1 Imipenem-susceptible,
More informationAntibiotics to Treat Multidrug-Resistant Bacterial Infections
Antibiotics to Treat Multidrug-Resistant Bacterial Infections Copyright 2017 Tetraphase Pharmaceuticals, Inc. C O M P A N Y P R E S E N T A T I O N A p r i l 2 0 1 7 TTPH Forward-Looking Statements and
More informationFirst identification of coexistence of bla NDM-1 and bla CMY-42 among Escherichia coli ST167 clinical isolates
Zhang et al. BMC Microbiology 2013, 13:282 RESEARCH ARTICLE Open Access First identification of coexistence of bla NDM-1 and bla CMY-42 among Escherichia coli ST167 clinical isolates Xueqing Zhang 1, Danping
More informationOIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : 2017-01-23 09:20:05 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Salmonellosis
More informationACCEPTED. Variant CTX-M-59 in a Neonatal Intensive Care Unit in. Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh,
AAC Accepts, published online ahead of print on 1 March 00 Antimicrob. Agents Chemother. doi:./aac.0-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationExtended-spectrum b-lactamases of Escherichia coli and Klebsiella pneumoniae screened by the VITEK 2 system
Journal of Medical Microbiology (2011), 60, 756 760 DOI 10.1099/jmm.0.024075-0 Extended-spectrum b-lactamases of Escherichia coli and Klebsiella pneumoniae screened by the VITEK 2 system Maria José Espinar,
More informationEmergence of Enterobacteriaceae co-producing CTX-M-15, ArmA and PMQR in Poland
Original papers Emergence of Enterobacteriaceae co-producing CTX-M-15, ArmA and PMQR in Poland Katarzyna Piekarska A,D,F, Katarzyna Zacharczuk C, Tomasz Wołkowicz C, Natalia Wolaniuk B, Magdalena Rzeczkowska
More informationCombatting AMR: diagnostics
Combatting AMR: diagnostics Professor Neil Woodford Antimicrobial Resistance & Healthcare Associated Infections (AMRHAI) Reference Unit Crown copyright Gonorrhoea: a paradigm for better diagnostics International
More informationBMC Research Notes. Open Access RESEARCH ARTICLE
DOI 10.1186/s13104-017-2467-2 BMC Research Notes RESEARCH ARTICLE Open Access Expansion of highly stable bla OXA-10 β-lactamase family within diverse host range among nosocomial isolates of Gram-negative
More informationLauren A. Darling1#, Ann M. Evans1, Kathleen A. Stellrecht1,2, Seela M. Nattanmai1,
JCM Accepted Manuscript Posted Online 20 September 2017 J. Clin. Microbiol. doi:10.1128/jcm.01185-17 Copyright 2017 American Society for Microbiology. All Rights Reserved. 1 JCM Letter to the Editor Submission
More informationHigh prevalence of plasmid-mediated 16S rrna methylase gene rmtb among Escherichia coli clinical isolates from a Chinese teaching hospital
RESEARCH ARTICLE Open Access Research article High prevalence of plasmid-mediated 16S rrna methylase gene rmtb among Escherichia coli clinical isolates from a Chinese teaching hospital Fang-you Yu 1, Dan
More informationJ. Appl. Environ. Biol. Sci., 5(12) , , TextRoad Publication
J. Appl. Environ. Biol. Sci., 5(2)262-268, 205 205, TextRoad Publication ISSN: 2090-4274 Journal of Applied Environmental and Biological Sciences www.textroad.com Study of routine antibiotic Resistance
More informationPHICO THERAPEUTICS. SASPject: First in a new class of novel biological antibacterials. Dr Adam Wilkinson R&D Manager
PHICO THERAPEUTICS SASPject: First in a new class of novel biological antibacterials Dr Adam Wilkinson R&D Manager Phico Founded 2000 by CEO Dr Heather Fairhead 20 employees Raised 13 M from 140 shareholders
More informationJOHN DEMPSEY HOSPITAL Farmington, Connecticut ANTIBIOTIC SUSCEPTIBILITY PROFILES for INPATIENT Bacterial Isolates
JOHN DEMPSEY HOSPITAL Farmington, Connecticut 2017 ANTIBIOTIC SUSCEPTIBILITY PROFILES for INPATIENT Bacterial Isolates **GROUPED BY CULTURE SOURCES** (data from 1/1/17 1/1/18) Prepared by: UCHC/JDH Antimicrobial
More informationDeveloping Novel Antibacterials to Treat Multi-drug Resistant Gram-negative Bacterial Infections. 13 th Needham Healthcare Conference April 8, 2014
Developing Novel Antibacterials to Treat Multi-drug Resistant Gram-negative Bacterial Infections 13 th Needham Healthcare Conference April 8, 2014 Forward Looking Statements Forward-Looking Statements
More informationCRE is not the first organism we ve had that has become resistant to antibiotics, so why is it so important? CRE resistance is complex because it can
1 Enterobacteriaceae are a large family of bacteria that are a normal part of a person's digestive system (2). Examples include Escherichia coli and species of the genera Klebsiella, Enterobacter, Serratia,
More informationMolecular epidemiology and drug resistant mechanism in carbapenem-resistant Klebsiella pneumoniae isolated from pediatric patients in Shanghai, China
RESEARCH ARTICLE Molecular epidemiology and drug resistant mechanism in carbapenem-resistant Klebsiella pneumoniae isolated from pediatric patients in Shanghai, China Xingyu Zhang 1, Di Chen 2, Guifeng
More informationExtended-Spectrum β-lactamases Producing Escherichia coli Strains Monitored Over 4 Years in The University Hospital in Košice, Slovakia
Current Research in Microbiology Original Research Paper Extended-Spectrum β-lactamases Producing Escherichia coli Strains Monitored Over 4 Years in The University Hospital in Košice, Slovakia 1 Viera
More informationAssociation of gyra mutation in Mycobacterium tuberculosis isolates with phenotypic ofloxacin resistance detected by resazurin microtiter assay
Association of gyra mutation in Mycobacterium tuberculosis isolates with phenotypic ofloxacin resistance detected by resazurin microtiter assay Dr Asho Ali King Abdul Aziz University Jeddah, Saudi Arabia
More informationby author How to effectively report laboratory findings to clinicians (Breakpoints and Interpretation)
How to effectively report laboratory findings to clinicians (Breakpoints and Interpretation) A Vatopoulos National School of Public Health & Central Public Health Laboratory KEELPNO Antibiotic Activity
More informationINNOVATIVE SCIENCE DRIVES ANTIBIOTIC LEADERSHIP. Corporate Presentation October 2018 Nasdaq: AKAO
INNOVATIVE SCIENCE DRIVES ANTIBIOTIC LEADERSHIP Corporate Presentation October 2018 Nasdaq: AKAO Forward Looking Statements This presentation contains forward-looking statements. All statements other than
More informationThe biomérieux solution. VITEK2 : A challenge with ESBL ESBL. Karen Bush
International Newsletter n 4 December 2003 Through the IDENTIFYING RESISTANCE Newsletter, biomérieux s ambition is to contribute to the awareness and progress in the field of resistance to antibiotics.
More informationDrug Susceptibility Pattern of Extraintestinal Pathogenic E.Coli Isolated from Various Clinical Specimens
International Journal of Research Studies in Microbiology and Biotechnology (IJRSMB) Volume 2, Issue 1, 2016, PP 22-27 ISSN 2454-9428 (Online) www.arcjournals.org Drug Susceptibility Pattern of Extraintestinal
More informationGenomic epidemiology of bacterial pathogens. Sylvain BRISSE Microbial Evolutionary Genomics, Institut Pasteur, Paris
Genomic epidemiology of bacterial pathogens Sylvain BRISSE Microbial Evolutionary Genomics, Institut Pasteur, Paris Typing Population genetics Analysis of strain diversity within species Aim: Local epidemiology?
More informationInternational Journal of Molecular and Clinical Microbiology
International Journal of Molecular and Clinical Microbiology 6(2) (2016) 687-692 International Journal of Molecular and Clinical Microbiology Prevalence of extended-spectrum ß-lactamase-producing Escherichia
More informationand IncN3-type plasmids in a Klebsiella pneumoniae isolate from Korea
AAC Accepted Manuscript Posted Online 7 December 2015 Antimicrob. Agents Chemother. doi:10.1128/aac.02532-15 Copyright 2015, American Society for Microbiology. All Rights Reserved. 1 2 Complete sequences
More informationMature Pseudomonas aeruginosa Biofilms Prevail Compared to Young Biofilms in the
AAC Accepts, published online ahead of print on 9 July 2012 Antimicrob. Agents Chemother. doi:10.1128/aac.00650-12 Copyright 2012, American Society for Microbiology. All Rights Reserved. 1 2 Mature Pseudomonas
More informationLink to article, DOI: / es Publication date: Document Version Publisher's PDF, also known as Version of record
Downloaded from orbit.dtu.dk on: Sep 07, 2018 Detection of mcr-1 encoding plasmid-mediated colistin-resistant Escherichia coli isolates from human bloodstream infection and imported chicken meat, Denmark
More informationABSTRACT IDENTIFICATION AND CHARACTERIZATION OF. CARBAPENEM HYDROLYSING β-lactamase KPC AMONG ENTEROBACTERIACEAE: A REPORT FROM NORTH INDIA
IDENTIFICATION AND CHARACTERIZATION OF CARBAPENEM HYDROLYSING β-lactamase KPC AMONG ENTEROBACTERIACEAE: A REPORT FROM NORTH INDIA ORGINAL ARTICLE, Vol-3 No.2 Asian Journal of Medical Science, Volume-3(2012)
More informationExperience of Transitioning from Phenotype to Genotype using Whole Genome Sequencing for Antimicrobial Resistance Surveillance.
Experience of Transitioning from Phenotype to Genotype using Whole Genome Sequencing for Antimicrobial Resistance Surveillance. Dr. Muna Anjum Molecular Lead: Antimicrobial Resistance and Enteric Pathogens,
More informationAre There Non-Carbapenem β-lactam Options for Treating ESBL Infections?
CIDEIM Are There Non-Carbapenem β-lactam Options for Treating ESBL Infections? Pranita D. Tamma, M.D., M.H.S. Assistant Professor, Pediatrics Director, Pediatric Antimicrobial Stewardship Program CIDEIM
More informationIndian J Med Res 140, November 2014, pp
Indian J Med Res 140, November 2014, pp 679-685 Comparison of pulsed-field gel electrophoresis & repetitive sequence-based PCR methods for molecular epidemiological studies of Escherichia coli clinical
More informationKey words: ESBLs; Ceftriaxone; Escherichia coli; MICs; Bacteremia
JCM Accepts, published online ahead of print on 16 April 2014 J. Clin. Microbiol. doi:10.1128/jcm.00716-14 Copyright 2014, American Society for Microbiology. All Rights Reserved. 1 1 2 Title: Determining
More information4.1.1 Multidrug resistance: Curing and Mechanism
4.0 Results and Discussion (Part II) 4.1 Results 4.1.1 Multidrug resistance: Curing and Mechanism Bacterial resistance to antibiotics is one of the most critical problems faced by the physicians all over
More informationCloning and Characterization of E. meningoseptica Beta Lactamase
Cloning and Characterization of E. meningoseptica Beta Lactamase Authors: Lindsey Purcell, Jessica Matts, Patricia Canaan* Department of Biochemistry and Molecular Biology Abstract Elizabethkingia meningoseptica
More informationA Verification Study for Implementing the Revised CLSI Breakpoints. Summary. Breakpoint Differences Cephalosporin Breakpoints for Enterobacteriaceae
A Verification Study for Implementing the Revised CLSI Breakpoints Jean B. Patel, PhD, D(ABMM) Deputy Director, Office of Antimicrobial Resistance National Center for Emerging and Zoonotic Infectious Disease
More informationBeef Industry Safety Summit Renaissance Austin Hotel 9721 Arboretum Blvd. Austin, TX March 1-3
1 USDA, Food Safety and Inspection Service Beef Industry Safety Summit - 2016 Renaissance Austin Hotel 9721 Arboretum Blvd. Austin, TX 78759 March 1-3 Uday Dessai MPH, MS, PhD Senior Public Health Advisor
More informationA Verification Study for Implementing the Revised CLSI Breakpoints. Summary. Glossary CDC 1
A Verification Study for Implementing the Revised CLSI Breakpoints Jean B. Patel, PhD, D(ABMM) Deputy Director, Office of Antimicrobial Resistance National Center for Emerging and Zoonotic Infectious Disease
More informationCME/SAM. Clinical Laboratory Detection of AmpC β-lactamase Does It Affect Patient Outcome?
Microbiology and Infectious Disease / Laboratory Detection of AmpC β-lactamase Clinical Laboratory Detection of AmpC β-lactamase Does It Affect Patient Outcome? Kenneth H. Rand, MD, 1 Bradley Turner, MD,
More informationDetection and molecular characterization of extended spectrum of beta lactamase (ESBL) producing Escherichia coli
ISSN: 2319-7706 Volume 2 Number 8 (2013) pp. 196-205 http://www.ijcmas.com Original Research Article Detection and molecular characterization of extended spectrum of beta lactamase (ESBL) producing Escherichia
More informationEpidemic Plasmid Carrying bla CTX-M-15 in Klebsiella penumoniae in China
Epidemic Plasmid Carrying bla CTX-M-15 in Klebsiella penumoniae in China Chao Zhuo 1 *, Xiao-qiang Li 1, Zhi-yong Zong 2, Nan-Shan Zhong 1 1 State Key Laboratory of Respiratory Diseases, Guangzhou Medical
More informationBiofilm Protocol Optimization For Pseudomonas aeruginosa. Introduction. Materials and Methods. Culture Media, Incubation Time, and Biofilm Measurement
Biofilm Protocol Optimization For Pseudomonas aeruginosa Culture Media, Incubation Time, and Biofilm Measurement Introduction In addition to the conventional arsenal of antibiotic resistance mechanisms
More informationOXA-type beta-lactamases among extended-spectrum cephalosporin-resistant Pseudomonas aeruginosa isolates in a university hospital in southern Taiwan
OXA-type J Microbiol ESBLs Immunol in P. Infect aeruginosa 2006;39:130-134 OXA-type beta-lactamases among extended-spectrum cephalosporin-resistant Pseudomonas aeruginosa isolates in a university hospital
More informationAbstract. Introduction
ORIGINAL ARTICLE 10.1111/j.1469-0691.2009.02893.x Comparative assessment of inoculum effects on the antimicrobial activity of amoxycillin clavulanate and piperacillin tazobactam with extended-spectrum
More informationInvestigation of Klebsiella pneumoniae Isolates Producing SHV-12 and SHV-11 β-lactamases in Korean Hospitals
J. Microbiol. Biotechnol. (2009), 19(2), 000 000 doi: 10.4014/jmb.0808.472 First published online 3 June 2009 Investigation of Klebsiella pneumoniae Isolates Producing SHV-12 and SHV-11 β-lactamases in
More informationPrevalence and spread of extendedspectrum b-lactamase-producing Enterobacteriaceae in Ngaoundere, Cameroon. Abstract. Editor: R.
RESEARCH NOTE BACTERIOLOGY Prevalence and spread of extendedspectrum b-lactamase-producing Enterobacteriaceae in Ngaoundere, Cameroon C. Lonchel Magoue 1, P. Melin 1, J. Gangoue-Pieboji 2, M.-C. Okomo
More information12 th EURL-AR Workshop
12 th EURL-AR Workshop The EURL protocol on mcr-1, -2, -3, -4, -5 Ana Rita Rebelo Research Assistant Colistin resistance in Enterobacteriaceae Mechanisms pmra/pmrb mutations not totally defined at this
More informationNovel genetic environment of the plasmid-mediated KPC-3 gene detected in Escherichia coli and Citrobacter freundii isolates from China
Eur J Clin Microbiol Infect Dis (2011) 30:575 580 DOI 10.1007/s10096-010-1124-7 ARTICLE Novel genetic environment of the plasmid-mediated KPC-3 gene detected in Escherichia coli and Citrobacter freundii
More informationCURRICULUM VITAE. Dr. James O. Ogutu, PhD
CURRICULUM VITAE Synopses: Personal details Areas of professional specialization Areas of research interest Academic profile Employment and work experience Key skills, abilities and clinical lab proficiencies
More informationINNOVATIVE SCIENCE DRIVES ANTIBIOTIC LEADERSHIP. Corporate Presentation June 2018 Nasdaq: AKAO
INNOVATIVE SCIENCE DRIVES ANTIBIOTIC LEADERSHIP Corporate Presentation June 2018 Nasdaq: AKAO Forward Looking Statements This presentation contains forward-looking statements. All statements other than
More informationReceived 24 June 2008/Returned for modification 12 August 2008/Accepted 14 September 2008
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Dec. 2008, p. 4268 4273 Vol. 52, No. 12 0066-4804/08/$08.00 0 doi:10.1128/aac.00830-08 Copyright 2008, American Society for Microbiology. All Rights Reserved. High
More informationNEXT STEP TO STOP THE SPREAD OF ANTIMICROBIAL RESISTANCE. Patricia Ruiz Garbajosa Servicio de Microbiología Hospital Universitario Ramón y Cajal
NEXT STEP TO STOP THE SPREAD OF ANTIMICROBIAL RESISTANCE Patricia Ruiz Garbajosa Servicio de Microbiología Hospital Universitario Ramón y Cajal ANTIMICROBIAL RESISTANCE AND HEALTHCARE-ASSOCIATED INFECTION
More informationPERANAN MIKROBIOLOGI DALAM DIAGNOSIS PENYAKIT INFEKSI. dr. Agus Eka Darwinata, Ph.D.
PERANAN MIKROBIOLOGI DALAM DIAGNOSIS PENYAKIT INFEKSI dr. Agus Eka Darwinata, Ph.D. CLINICAL MICROBIOLOGY Clinical microbiology is the discipline of detection, characterization, and quantification of
More informationEvaluation of the molecular epidemiology of ESBL-producing Escherichia coli associated with blood stream infections in China
Evaluation of the molecular epidemiology of ESBL-producing Escherichia coli associated with blood stream infections in China Anna Olsson Master Degree Project in Infection Biology, 30 credits. Spring 2017
More informationANTIMICROBIAL SUSCEPTIBILITY TESTING: ADVANCED
ANTIMICROBIAL SUSCEPTIBILITY TESTING: ADVANCED Romney Humphries, Ph.D., DABMM Section Chief, Clinical Microbiology University of California Los Angeles Lost Angeles, CA Susan E. Sharp, Ph.D., DABMM, FAAM
More informationORIGINAL ARTICLE /j x
ORIGINAL ARTICLE 10.1111/j.1469-0691.2008.02030.x Metallo-b-lactamase-producing Pseudomonas aeruginosa isolated from a large tertiary centre in Kenya J. D. D. Pitout 1,2,3, G. Revathi 4, B. L Chow 1, B.
More informationEducational Workshops 2016
Educational Workshops 2016 Case One: Outbreak of CPE We are grateful to Suzy Fitzgerald, Consultant Microbiologist, St Vincent s University Hospital, Dublin for composing this case. 2011 Spring 2011 Sporadic
More informationEzy MIC Strip FEATURES AND ADVANTAGES
Imipenem with & without EDTA Ezy MIC Strips (IPM+EDTA/IPM) (Imipenem + EDTA: 1-64 mcg/ml) (Imipenem : 4-256 mcg/ml) Antimicrobial Susceptibility Testing For In Vitro Diagnostic use EM078 Not for Medicinal
More informationCharacterization, Antibiotic Resistance Pattern and Detection of Metalloβ-lactamases and Amp C in Pseudomonas aeruginosa in a Tertiary Care Hospital
Original Research Characterization, Antibiotic Resistance Pattern and Detection of Metalloβ-lactamases and Amp C in Pseudomonas aeruginosa in a Tertiary Care Hospital Amit Rajshekar Ugargol 1, Shilpa Karnum
More information