A Sub-picogram Quantification Method for Desmopressin in Plasma using the SCIEX Triple Quad 6500 System

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "A Sub-picogram Quantification Method for Desmopressin in Plasma using the SCIEX Triple Quad 6500 System"

Transcription

1 A Sub-picogram Quantification Method for Desmopressin in Plasma using the SCIEX Triple Quad 6500 System A high-throughput method for detecting ultra-low levels (0.5 pg/ml) of a therapeutic peptide in human plasma using an SCIEX Triple Quad 6500 LC-MS/MS System and UHPLC Chromatography Rahul Baghla 1, Swati Guttikar 2, Dharmesh Patel 2, Abhishek Gandhi 2, Anoop Kumar 1, and Manoj Pillai 1 1 SCIEX, 121, Udyog Vihar, Phase IV, Gurgaon, Haryana, India 2 Veeda Clinical Research India, Ahmadabad, India Key challenges of desmopressin quantitation Impaired sensitivity in complex matrices Very low-level peptide detection (sub-pg/ml) can be suppressed by high background and competing ions in biological samples. Poor data quality Precision and accuracy can be compromised at low peptide levels, giving results below accepted bioanalytical standards. Key benefits of peptide quantitation on the SCIEX Triple Quad 6500 LC-MS/MS System Technology High sensitivity Very low level peptide detection in human plasma (at sub pg/ml concentrations) is enabled by IonDrive System Technology. Unique features of the SCIEX Triple Quad 6500 System for low-level peptide detection IonDrive Turbo V Source Increased ionization efficiency and heat transfer contribute to sensitivity enhancements, including improved signal-to-noise. IonDrive QJet Ion Guide Increased ion sampling improves method efficiency and ruggedness. IonDrive High Energy Detector Innovative detector technology boosts dynamic range and sensitivity. Excellent precision and accuracy at the LOQ level Data quality (for LOQ, LQC, MQC and HQC levels) met or exceeded USFDA bioanalytical method validation criteria. High throughput High sensitivity was achieved under high-flow conditions (0.750 ml/min), optimal for multi-sample analysis. Figure 2: Unique features of SCIEX Triple Quad 6500 System. Figure 1: SCIEX Triple Quad 6500 System. Introduction Low-level peptide detection has a number of applications in clinical studies and in the pharmaceutical discovery and development processes, highlighting the increasing relevance of sensitive and selective mass spectrometric platforms in the bioanalytical laboratory. Regulatory requirements demand intensive and rigorous quantitation of therapeutic peptides during pharmacokinetic, bioequivalence, and metabolic studies. In addition, drug discovery and development strategies seek to monitor and quantitate peptide biomarkers in complex biological samples, necessitating highly-selective separations of low concentration analytes from high background noise and prominent levels of competing ions. The SCIEX Triple Quad 6500 LC-MS/MS System, equipped with IonDrive System Technology for enhanced detector performance, has demonstrated particular strength in the detection of low-

2 level amounts of small molecules, and in this study, we extend the augmented signal-to-noise, broad dynamic range, and the efficient method development capacities of the SCIEX Triple Quad 6500 System to the detection of sub-picogram levels of a therapeutic peptide under high-throughput conditions. We have developed a reliable, fast, and sensitive method for the detection of a nine-amino-acid peptide, desmopressin (1-desamino-8-D-arginine, vasopressin), which is structurally similar to the hormone arginine vasopressin, but contains a deaminated first amino acid and dextro-arginine (rather than levo-) in the eighth position. Therapeutically, desmopressin reduces urine production, restricting water elimination from the kidneys by binding to the V2 receptors in renal-collecting ducts, thereby facilitating increased reabsorption. The longer half-life of desmopressin over vasopressin offers some therapeutic advantages, and typical doses of desmopressin to treat diabetes insipidus and bedwetting range between to 1.20 mg per day, resulting in very low plasma concentrations. In this bioanalytical study, we have established a sensitive and selective LC-MS/MS method for the quantitation of desmopressin in human plasma, detecting peptide levels as low as pg/ml with excellent accuracy and precision. This technique should facilitate additional mass spectrometric method development for accurate quantitation of a range of therapeutic peptides in biological matrices on the SCIEX Triple Quad 6500 System. Materials and Methods Sample Preparation Plasma samples (1000 µl) containing 2% desmopressin standard and 50µL internal standard were vortexed and spiked with 50µL of orthophosphoric acid (OPA). Samples were extracted on weak cation exchange cartridges conditioned with methanol followed by 100mM ammonium acetate solution. After loading, samples were washed in three steps: 1) 2% OPA:methanol (80:20 v/v); 2) 2% NaOH:Methanol (60:40 v/v); and 3) water:methanol (60:40 v/v). Analytes were eluted with 5% acetic acid in methanol, dried under nitrogen at 40 ºC, and reconstituted with 0.1% acetic acid (150 µl) prior to analysis by mass spectrometry. Chromatography LC System: GL Sciences LC 800 System Column: Agilent 300 Extend C 18 (150 x 2.1 mm, 3.5 μm) Column Temp.: 40 C Injection: 50 μl Flow Rate: ml/min Mobile phase: A) water, 0.1 % acetic acid B) acetonitrile, 0.1% acetic acid Gradient: Time/min A% B% Mass Spectrometry Analysis of desmopressin and desmopressin-d 5 required different mass spectrometry settings (Table 1). The MRM transition monitored for desmopressin was m/z 525.4/328.0 and 537.9/328.0 at a dwell time of 50 msec. Five replicate injections were performed at all concentrations. Data System: Interface: SCIEX Triple Quad 6500 System IonDrive Turbo V Source in positive ion mode Figure 3. Structure of desmopressin. Table 1. Compound-dependent parameters for desmopressin anddesmopressin-d 5 on the SCIEX Triple Quad 6500 System Instrument Parameter Desmopressin Desmopressin-d5 CUR TEM 600 C 600 C ISV GS GS CAD DP EP CE CXP Figure 4. Structure of internal standard, desmopressin-d 5.

3 Data processing All SCIEX Triple Quad 6500 System data was processed using MultiQuant Software.. The concentration curves were analyzed using a linear fit with a 1/x 2 weighting. Data acquired on the SCIEX Triple Quad 6500 System was processed using the quantitation tools within Analyst 1.6 Software. Results and Discussion Method analysis and data quality The desmopressin quantitative assay was validated by generating an internal standard curve using standards alone and standards spiked into human plasma. Left side pane of Figure 5 shows representative peaks for A) blank extract, B) plasma spiked with 0.5 pg/ml desmopressin and the right side pane of Figure 5 shows the MRM response from the internal standard. Standard concentrations varied from 0.5 to 100 pg/ml generating an LLOQ in plasma of 0.5 pg/ml resulting in a signal to noise ratio of 60.7 (Figure 6). Reproducibility of the assay was assessed by multiple technical replicates of the same sample (n = 6, Figure 7) on an LLOQ quality control sample of 0.5 pg/ml. The calibration curve for desmopressin in plasma shows excellent linearity over 2.5 orders of magnitude concentration range with an r value of >0.99 (Figure 8). Figure 6: High signal-to-noise ratio for desmopressin.. The signalto-noise ratio was calculated for desmopressin extracted from plasma at LLOQ level (0.500 pg/ml in plasma, S/N = 60.7). Figure 5. Desmopressin MRM signal (shown in left side ) panes for multiple concentrations and Desmopressin D5 MRM signal (Shown in right side panes). Figure 7: Desmopressin technical replicates. Chromatograms of six LLOQ quality control samples (0.502 pg/ml) for precision and accuracy calculations are shown (Table 2).

4 The data collected for a single calibration curve are presented in Table 2. Analyte retention time and internal standard peak retention times were consistent, with both eluting at approximately 2.6 min. The calculated concentration correlates well with the actual spikedanalyte concentration in plasma matrix with a percent accuracy of the standard curve very close to 100% for all concentrations of standard, and the quality control samples had a percent accuracy of 110%. Table 3 shows the individual statistics for three separate batch runs of desmopressin. Data from Table 2 are taken from Batch 3. Table 4 shows the mean values for the percent accuracy and % CV for three separate batch runs. For the LLOQ quality control, the mean accuracy was calculated to be 108% with a %CV of 10.5%. The percent recovery and plasma matrix effect were evaluated by comparing the peak areas for standard curve samples with and without plasma (Table 5). The mean percent recovery was calculated to be 93%. The recovery of the internal standard was calculated to be 78% (Table 6). Figure 8: Calibration curve of desmopressin in plasma from pg/ml to pg/ml. The method has shown excellent linearity over the concentration range with r = Table 2. Data quality analysis of desmopressin from human plasma samples Sample ID Sample Type Analyte Retention Time (min) Analyte Peak Area IS Retention Time (min) IS Peak Area Area Ratio Analyte Conc. (pg/ml) Calculated Conc. (pg/ml) % Accuracy AQM Unknown , N/A N/A BLANK 01 Blank #DIV/0! N/A #DIV/0! N/A BLANK+IS 01 Unknown ,418 0 N/A No Peak N/A STD A 01 Standard , , STD B 01 Standard , , STD C 01 Standard , , STD D 01 Standard , , STD E 01 Standard , , STD F 01 Standard , , STD G 01 Standard , , STD H 01 Standard , , LLOQ QC 01 Qual. Control , , LQC 01 Qual. Control , , MQC 01 Qual. Control , , HQC 01 Qual. Control , , LLOQ QC 02 Qual. Control , , LQC 02 Qual. Control 2.6 5, , MQC 02 Qual. Control , , HQC 02 Qual. Control , , LLOQ QC 03 Qual. Control , , LQC 03 Qual. Control , , MQC 03 Qual. Control , , HQC 03 Qual. Control , , LLOQ QC 04 Qual. Control , , LQC 04 Qual. Control , , MQC 04 Qual. Control , , HQC 04 Qual. Control , , LLOQ QC 05 Qual. Control , , LQC 05 Qual. Control , , MQC 05 Qual. Control , , HQC 05 Qual. Control , , LLOQ QC 06 Qual. Control , , LQC 06 Qual. Control , , MQC 06 Qual. Control , , HQC 06 Qual. Control , , Table 2. Full analysis of precision and accuracy measurements for desmopressin (batch 1 samples) in human plasma.

5 Table 3: Precision and accuracy for multi-batch desmopressin technical replicates DESMOPRESSIN Nominal Concentration (pg/ml) LLOQ QC LQC MQC HQC PA BATCH Mean S.D (+/-) C.V. (%) % Nominal N PA BATCH Mean S.D (+/-) C.V. (%) % Nominal N PA BATCH Mean S.D (+/-) C.V. (%) % Nominal N Table 3: Precision and accuracy calculations for individual batches of desmopressin samples. Table 4. Precision and accuracy of desmopressin technical replicates measured as a single group DESMOPRESSIN Nominal Concentration (pg/ml) LLOQ QC LQC MQC HQC Mean S.D (+/-) C.V. (%) % Nominal N Table 4: Mean precision and accuracy calculations for desmopressin for three batches of measurements from different days. Table 5. Recovery of desmopressin from human plasma, batch 03 LQC RESPONSE MQC RESPONSE HQC RESPONSE Sample ID Extracted Unextracted Extracted Unextracted Extracted Unextracted 01, 013 5,923 6, , , , ,315 02, 014 6,645 6, , , , ,138 03, 015 6,572 6, , , , ,730 04, 016 5,879 6, , , , ,201 05, 017 5,823 6, , , , ,383 06, 018 5,263 5, , , , ,647 Mean 6, , , , ,735.7 S.D , , , C.V N % Recovery Mean SD (+/-) CV(%) N 3 Table 5: Recovery of desmopressin from plasma at three different concentrations, LQC, MQC and HQC, was 92.72%.

6 Conclusions A highly sensitive and high-throughput bioanalytical method was developed and validated for the detection of ultra-low-levels of the therapeutic peptide, desmopressin, in human plasma on the SCIEX Triple Quad 6500 LC-MS/MS System. Method sensitivity for desmopressin detection was exceptional (0.5 pg/ml or 2.5 fg on column), and demonstrated high-reproducibility and cost effectiveness with good precision and accuracy. Analyte recovery is 92.7%, even under high-throughput conditions. Total run time for each sample was only 5 min, using a flow rate rapid enough for high-throughput analysis in the bioanalytical laboratory. References Friedman, F and Weiss JP. Desmopressin in the treatment of nocturia: clinical evidence and experience. Therapeutic Advances in Urology. 2013; 5(6): Neudert, L, Zaugg, M, Wood, S, Struwe, P. A high sensitivity dual solid phase extraction LC-MS/MS assay for the determination of the therapeutic peptide desmopressin in human plasma. Celerion white paper. Acknowledgements The authors are indebted to Dr.Venu Madhav, Chief Operating Officer (COO), Veeda Clinical Research, India, for his encouragement and support for the successful completion of the work. AB Sciex is doing business as SCIEX AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX is being used under license. RUO-MKT A Headquarters 500 Old Connecticut Path Framingham, MA USA Phone sciex.com International Sales For our office locations please call the division headquarters or refer to our website at sciex.com/offices

A Sub-picogram Quantification Method for Desmopressin in Plasma using the AB SCIEX Triple Quad 6500 System

A Sub-picogram Quantification Method for Desmopressin in Plasma using the AB SCIEX Triple Quad 6500 System A Sub-picogram Quantification Method for Desmopressin in Plasma using the AB SCIEX Triple Quad 6500 System A high-throughput method for detecting ultra-low levels (0.5 pg/ml) of a therapeutic peptide in

More information

Improving the Detection Limits for Highly Basic Neuropeptides Using CESI-MS

Improving the Detection Limits for Highly Basic Neuropeptides Using CESI-MS Improving the Detection Limits for Highly Basic Neuropeptides Using CESI-MS Stephen Lock SCIEX UK Overview Who Should Read This: Senior Scientists and Lab Directors with an Interest in Protein Analysis

More information

Analysis of the Massachusetts Cannabis Pesticides List Using the SCIEX QTRAP System

Analysis of the Massachusetts Cannabis Pesticides List Using the SCIEX QTRAP System Analysis of the Massachusetts Cannabis Pesticides List Using the SCIEX QTRAP 6500+ System Evoking the power of MS3 to reduce matrix background in cannabis extracts Craig M. Butt 1, Robert Di Lorenzo 2,

More information

High Levels of Sensitivity and Robustness for Complex and Demanding Matrices SCIEX TRIPLE QUAD 5500 SYSTEM

High Levels of Sensitivity and Robustness for Complex and Demanding Matrices SCIEX TRIPLE QUAD 5500 SYSTEM High Levels of Sensitivity and Robustness for Complex and Demanding Matrices SCIEX TRIPLE QUAD 5500 SYSTEM The SCIEX Triple Quad 5500 System is designed to deliver a high level of sensitivity and robustness

More information

Universal Solution for Monoclonal Antibody Quantification in Biological Fluids Using Trap-Elute MicroLC-MS Method

Universal Solution for Monoclonal Antibody Quantification in Biological Fluids Using Trap-Elute MicroLC-MS Method Universal Solution for Monoclonal Antibody Quantification in Biological Fluids Using Trap-Elute MicroLC-MS Method Featuring the SCIEX QTRAP 6500+ LC-MS/MS System with OptiFlow Turbo V source and M5 MicroLC

More information

OptiFlow Quant Solution for High-Sensitivity Quantitation

OptiFlow Quant Solution for High-Sensitivity Quantitation OptiFlow Quant Solution for High-Sensitivity Quantitation The Intelligent Union of Sensitivity, Ease-of-Use, and Robustness MicroFlow Sensitivity Analytical Flow Usability Transform Your Quantitation Microflow

More information

Ultra-Sensitive Quantification of Trastuzumab Emtansine in Mouse Plasma using Trap-Elute MicroLC MS Method

Ultra-Sensitive Quantification of Trastuzumab Emtansine in Mouse Plasma using Trap-Elute MicroLC MS Method Ultra-Sensitive Quantification of Trastuzumab Emtansine in Mouse Plasma using Trap-Elute MicroLC MS Method Featuring SCIEX QTRAP 6500+ System with OptiFlow Turbo V source and M5 MicroLC system Lei Xiong,

More information

MultiQuant Software 2.0 with the SignalFinder Algorithm

MultiQuant Software 2.0 with the SignalFinder Algorithm MultiQuant Software 2.0 with the SignalFinder Algorithm The Next Generation in Quantitative Data Processing Quantitative analysis using LC/MS/MS has benefited from a number of advancements that have led

More information

ACCURACY THE FIRST TIME. Bring Your Esoteric Assays In-House Routinely SCIEX DIAGNOSTICS MASS SPECTROMETERS

ACCURACY THE FIRST TIME. Bring Your Esoteric Assays In-House Routinely SCIEX DIAGNOSTICS MASS SPECTROMETERS ACCURACY THE FIRST TIME Bring Your Esoteric Assays In-House Routinely SCIEX DIAGNOSTICS MASS SPECTROMETERS Your routine diagnostic testing lab requires accurate trace level quantitation of multiple compounds

More information

ACCURACY THE FIRST TIME. Reliable testing with performance, productivity and value combined AB SCIEX 3200MD SERIES MASS SPECTROMETERS

ACCURACY THE FIRST TIME. Reliable testing with performance, productivity and value combined AB SCIEX 3200MD SERIES MASS SPECTROMETERS ACCURACY THE FIRST TIME Reliable testing with performance, productivity and value combined AB SCIEX 3200MD SERIES MASS SPECTROMETERS Reliability, reproducibility, and confidence in your data Your routine

More information

A Sensitive and Robust Method for the Quantification of Goserelin in Plasma Using Micro-Elution Plates

A Sensitive and Robust Method for the Quantification of Goserelin in Plasma Using Micro-Elution Plates A Sensitive and Robust Method for the Quantification of Goserelin in Plasma Using Micro-Elution Plates Tirupateswara B. Rao, P. Veeranjaneyulu, Sudarshan Mantha, and Dr. Gopal Vaidyanathan Waters Corporation,

More information

High Speed, Ultra-High Sensitivity, and Robustness Needed for the Quantitation of Pharmaceuticals in Blood Plasma. Application Note. Authors.

High Speed, Ultra-High Sensitivity, and Robustness Needed for the Quantitation of Pharmaceuticals in Blood Plasma. Application Note. Authors. High Speed, Ultra-High Sensitivity, and Robustness Needed for the Quantitation of Pharmaceuticals in Blood Plasma Application Note Drug discovery and development: Drug metabolism and pharmacokinetics (DMPK)

More information

See More, Much More Clearly

See More, Much More Clearly TRIPLETOF 6600 SYSTEM WITH SWATH ACQUISITION 2.0 See More, Much More Clearly NEXT-GENERATION PROTEOMICS PLATFORM Every Peak. Every Run. Every Time. The Next-generation Proteomics Platform is here: The

More information

Clinical IVD LC-MS/MS Systems Class l Medical Devices and CE-marked LC-MS/MS In Vitro Diagnostic Systems

Clinical IVD LC-MS/MS Systems Class l Medical Devices and CE-marked LC-MS/MS In Vitro Diagnostic Systems Clinical IVD LC-MS/MS Systems Class l Medical Devices and CE-marked LC-MS/MS In Vitro Diagnostic Systems Diagnostics Capitalize on the Benefits of Mass Spectrometry for Your Clinical Laboratory Mass spec

More information

Simplifying CE-SDS Data Processing

Simplifying CE-SDS Data Processing Simplifying CE-SDS Data Processing Approach for Mitigating Product Peak Migration Time Drift Samuel Shepherd 1, KieranKumar Mistry 1, Whitney Lane Smith 2, Nicholas Bond 1, Vivian Lindo 1, Sam Fox 3, Jim

More information

Improving Sensitivity in Bioanalysis using Trap-and-Elute MicroLC-MS

Improving Sensitivity in Bioanalysis using Trap-and-Elute MicroLC-MS Improving Sensitivity in Bioanalysis using Trap-and-Elute MicroLC-MS Using the SCIEX M3 MicroLC system for Increased Sensitivity in Antibody Quantitation Remco van Soest and Lei Xiong SCIEX, Redwood City,

More information

The Overview of LC-MS Quantitative Solutions for Biotherapeutic Analysis

The Overview of LC-MS Quantitative Solutions for Biotherapeutic Analysis The Overview of LC-MS Quantitative Solutions for Biotherapeutic Analysis Featuring SCIEX Instrumentation and Technologies Lei Xiong, Elliott Jones SCIEX, Redwood City, California, USA Figure 1. Summary

More information

SCIEX OS Software One Touch Productivity

SCIEX OS Software One Touch Productivity SCIEX OS Software One Touch Productivity Accuracy, Simplicity and Efficiency What SCIEX OS Software is all About Mass spectrometry and the data challenge Mass spectrometry continues to provide more comprehensive

More information

Made for Routine Food, Environment, and Forensic Testing. The X500R QTOF System

Made for Routine Food, Environment, and Forensic Testing. The X500R QTOF System Made for Routine Food, Environment, and Forensic Testing. The X500R QTOF System RUO-MKT-03-2843-A We knew what you were looking for in a mass spec system, and we delivered on all fronts, starting with

More information

Improving Sensitivity for an Immunocapture LC-MS Assay of Infliximab in Rat Plasma Using Trap-and-Elute MicroLC-MS

Improving Sensitivity for an Immunocapture LC-MS Assay of Infliximab in Rat Plasma Using Trap-and-Elute MicroLC-MS Improving Sensitivity for an Immunocapture LC-MS Assay of in Rat Plasma Using Trap-and-Elute MicroLC-MS Using the SCIEX M3 MicroLC system for Increased Sensitivity in Antibody Quantitation Remco van Soest

More information

Benefits of 2D-LC/MS/MS in Pharmaceutical Bioanalytics

Benefits of 2D-LC/MS/MS in Pharmaceutical Bioanalytics Application Note Small Molecule Pharmaceuticals Benefits of 2D-LC/MS/MS in Pharmaceutical Bioanalytics Avoiding Matrix Effects Increasing Detection Sensitivity Authors Jonas Dinser Daiichi-Sankyo Europe

More information

The LC-MS/MS Workhorse. SCIEX 4500 Series Mass Spectrometers

The LC-MS/MS Workhorse. SCIEX 4500 Series Mass Spectrometers The LC-MS/MS Workhorse SCIEX 4500 Series Mass Spectrometers The SCIEX 4500 Series takes the legendary API 4000 platform and intelligently re-engineers it to set a new benchmark for reliable quantitation.

More information

MultiQuant Software Version 3.0

MultiQuant Software Version 3.0 MultiQuant Software Version 3.0 Improving Data Quality and Processing Throughput with Better Peak Integration, Quantitative and Qualitative Compound Review for the Analysis of Food, Drinking Water, and

More information

Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS

Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS SCIEX Triple Quad 4500 LC/MS/MS system and Beckman Coulter Biomek 4000 Workstation Lekh Sharma 1, Michael

More information

AB SCIEX TripleTOF 5600 SYSTEM. High-resolution quant and qual

AB SCIEX TripleTOF 5600 SYSTEM. High-resolution quant and qual AB SCIEX TripleTOF 5600 SYSTEM High-resolution quant and qual AB SCIEX TripleTOF 5600 SYSTEM AB SCIEX is a global leader in the development of life science analytical technologies that help answer complex

More information

On-line SPE-LC/MS/MS to Detect Organonitrogen and Triazine Pesticides at 10ng/L in Drinking Water

On-line SPE-LC/MS/MS to Detect Organonitrogen and Triazine Pesticides at 10ng/L in Drinking Water application note On-line SPE-LC/MS/MS of Pesticides On-line SPE-LC/MS/MS to Detect Organonitrogen and Triazine Pesticides at 10ng/L in Drinking Water API 3200 LC/MS/MS System Overview The feasibility of

More information

Quantitatitive Analysis of Phosphorothioate Oligonucleotide in Human Plasma Using LC-MS/MS with On-Line Extraction

Quantitatitive Analysis of Phosphorothioate Oligonucleotide in Human Plasma Using LC-MS/MS with On-Line Extraction Laixin Wang, Sherry Liu, Qiuying Zhu, Scott Reuschel and Min Meng Tandem Labs Quantitatitive Analysis of Phosphorothioate Oligonucleotide in Human Plasma Using LC-MS/MS with On-Line Extraction Introduction

More information

Pharmacokinetic Analysis of the mab Adalimumab by ELISA and Hybrid LBA/LC/MS: A Comparison Study

Pharmacokinetic Analysis of the mab Adalimumab by ELISA and Hybrid LBA/LC/MS: A Comparison Study Pharmacokinetic Analysis of the mab Adalimumab by ELISA and Hybrid LBA/LC/MS: A Comparison Study Featuring the SCIEX BioBA Solution Xi Qu 1, Shuyu Hou 1, Meghan McCann 1, Caroline Becker 1, Xun Wang 1,

More information

Rapid Peptide Catabolite ID using the SCIEX Routine Biotransform Solution

Rapid Peptide Catabolite ID using the SCIEX Routine Biotransform Solution Rapid Peptide Catabolite ID using the SCIEX Routine Biotransform Solution Rapidly Identify Major Catabolites with the SCIEX X500R QTOF System and MetabolitePilot TM 2.0 Software Ian Moore and Jinal Patel

More information

World-leading LC/MS/MS technology for quantitation and identification API LC/MS/MS System

World-leading LC/MS/MS technology for quantitation and identification API LC/MS/MS System World-leading LC/MS/MS technology for quantitation and identification API 3000 LC/MS/MS System rel Accelerate your drug development cycle The API 3000 LC/MS/MS System leads the field in sensitivity, specificity,

More information

Multiplexing Two Different Food Residue Methods using HILIC and Reversed Phase Chromatography in the Same LC-MS/MS Run

Multiplexing Two Different Food Residue Methods using HILIC and Reversed Phase Chromatography in the Same LC-MS/MS Run Multiplexing Two Different Food Residue Methods using HILIC and Reversed Phase Chromatography in the Same LC-MS/MS Run Adrian Taylor and André Schreiber AB SCIEX Concord, Ontario (Canada) Overview Multiplexing

More information

10. Validated Normal Phase HPLC Method for the Determination. Fulvestrant is primarily used in the treatment of hormone receptor

10. Validated Normal Phase HPLC Method for the Determination. Fulvestrant is primarily used in the treatment of hormone receptor 229 10. Validated Normal Phase HPLC Method for the Determination of Fulvestrant in Pharmaceutical Dosage Forms 10.1 Introduction Fulvestrant is primarily used in the treatment of hormone receptor positive

More information

METABOLOMICS. Biomarker and Omics Solutions FOR METABOLOMICS

METABOLOMICS. Biomarker and Omics Solutions FOR METABOLOMICS METABOLOMICS Biomarker and Omics Solutions FOR METABOLOMICS The metabolome consists of the entirety of small molecules consumed or created in the metabolic processes that sustain life. Metabolomics is

More information

Validation of Analytical Method of Irbesartan Plasma in Vitro by High Performance Liquid Chromatography-Fluorescence

Validation of Analytical Method of Irbesartan Plasma in Vitro by High Performance Liquid Chromatography-Fluorescence Journal of Life Sciences 6 (2012) 726-731 Validation of Analytical Method of Irbesartan Plasma in Vitro by High Performance Liquid Chromatography-Fluorescence Harmita, Yahdiana Harahap and I. Kadek Arya

More information

The Importance of Temperature Control in Capillary Electrophoresis of Carbohydrates

The Importance of Temperature Control in Capillary Electrophoresis of Carbohydrates The Importance of Temperature Control in Capillary Electrophoresis of Carbohydrates Introducing Temperature Gradient Capillary Electrophoresis Andras Guttman, 1 Marton Szigeti 2 and Jeff Chapman 1 1 SCIEX

More information

Tyler Trent, Applications Sales Specialist; Teledyne Tekmar Page 1

Tyler Trent, Applications Sales Specialist; Teledyne Tekmar Page 1 Application Note Method Validation of the AutoMate-Q40 for Pesticide Residues in Grapes Tyler Trent, Applications Sales Specialist; Teledyne Tekmar Page 1 Abstract The QuEChERS (Quick-Easy-Cheap-Effective-Rugged-Safe)

More information

Quantitation of Microcystins and Nodularins in Water Samples Using LC-MS/MS

Quantitation of Microcystins and Nodularins in Water Samples Using LC-MS/MS Quantitation of Microcystins and Nodularins in Water Samples Using LC-MS/MS Craig Butt1, April Quinn-Paquet1, and Christopher Borton2 SCIEX (Framingham, MA) 2 SCIEX (Redwood City, CA) 1 Overview This application

More information

Exploring Extra Sensitivity Using ionkey/ms with the Xevo G2-XS Q-Tof HRMS for Small Molecule Pharmaceutical Analysis in Human Plasma

Exploring Extra Sensitivity Using ionkey/ms with the Xevo G2-XS Q-Tof HRMS for Small Molecule Pharmaceutical Analysis in Human Plasma Exploring Extra Sensitivity Using ionkey/ms with the Xevo G2-XS Q-Tof HRMS for Small Molecule Pharmaceutical Analysis in Human Plasma Yun Wang Alelyunas, Mark D. Wrona, Jim Murphy, Angela Doneanu, Gregory

More information

Time, min

Time, min Determination of Melamine in Full Fat milk. Introduction: This document describes the first experiments to determine Melamine in raw cow milk (8-10% fat). Due to the limited time the application is divided

More information

Repeatability of C100ht Biologics Analyzer, for High Throughput Glycan Screening

Repeatability of C100ht Biologics Analyzer, for High Throughput Glycan Screening Repeatability of C100ht Biologics Analyzer, for High Throughput Glycan Screening Marcia Santos, Tingting Li, Mervin Gutierrez, Anna Lou, and Clarence Lew SCIEX Separations, Brea, CA Introduction The ability

More information

Determination of Pesticide Residue in Vegetables Using the AutoMate-Q40. Application Note. Abstract. Introduction

Determination of Pesticide Residue in Vegetables Using the AutoMate-Q40. Application Note. Abstract. Introduction Application Note Abstract Determination of Pesticide Residue in Vegetables Using the AutoMate-Q40 Page 1 QuEChERS is a Quick-Easy-Cheap-Effective-Rugged-Safe extraction method that has been developed for

More information

Background. Ferring Pharmaceuticals

Background. Ferring Pharmaceuticals Background Ferring Pharmaceuticals Founded in 195 and have since then worked with peptides Today, focus are on peptides as well as proteins Many of our peptides are agonists targeting the oxytocin or vasopressin

More information

Application Note. Abstract. Introduction. Experimental Instrument Conditions. Evaluation of Prohibited Dyes in Food : By An Automated Extraction

Application Note. Abstract. Introduction. Experimental Instrument Conditions. Evaluation of Prohibited Dyes in Food : By An Automated Extraction Application Note Abstract Evaluation of Prohibited Dyes in Food : By An Automated Extraction By: Tyler Trent P a g e 1 The Sudan dyes Sudan I, Sudan II, Sudan III and Sudan IV all belong to the compound

More information

Introduction. Benefits of the SWATH Acquisition Workflow for Metabolomics Applications

Introduction. Benefits of the SWATH Acquisition Workflow for Metabolomics Applications SWATH Acquisition Improves Metabolite Coverage over Traditional Data Dependent Techniques for Untargeted Metabolomics A Data Independent Acquisition Technique Employed on the TripleTOF 6600 System Zuzana

More information

Robustness of the Agilent Ultivo Triple Quadrupole LC/MS for Routine Analysis in Food Safety

Robustness of the Agilent Ultivo Triple Quadrupole LC/MS for Routine Analysis in Food Safety Technical Overview Robustness of the Agilent Ultivo Triple Quadrupole LC/MS for Routine Analysis in Food Safety Authors Mark Sartain, Theresa Sosienski, and Dan Hui Dorothy Yang Agilent Technologies, Inc.

More information

Quantification of Host Cell Protein Impurities Using the Agilent 1290 Infinity II LC Coupled with the 6495B Triple Quadrupole LC/MS System

Quantification of Host Cell Protein Impurities Using the Agilent 1290 Infinity II LC Coupled with the 6495B Triple Quadrupole LC/MS System Application Note Biotherapeutics Quantification of Host Cell Protein Impurities Using the Agilent 9 Infinity II LC Coupled with the 6495B Triple Quadrupole LC/MS System Authors Linfeng Wu and Yanan Yang

More information

Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE

Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE Application Note AN880 Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE EXPRESS ABN Prior to LC-MS/MS Analysis Page 1 Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE EXPRESS ABN Prior

More information

DRUG DISCOVERY & 2 September 2010

DRUG DISCOVERY & 2 September 2010 LC-MS/MS QUANTITATION IN DRUG DISCOVERY & DEVELOPMENT Ludmila Alexandrova 2 September 2010 1 For personal use only. Please do not reuse or reproduce without the author s permission. 2 Presentation Outline

More information

Quantitative analysis of Adalimumab using nano-surface and molecular-orientation limited (nsmol) proteolysis and LC/MS/MS

Quantitative analysis of Adalimumab using nano-surface and molecular-orientation limited (nsmol) proteolysis and LC/MS/MS PO-CON1686E Quantitative analysis of Adalimumab using nano-surface and molecular-orientation limited (nsmol) ASMS 2016 WP 634 Deepti Bhandarkar (1), Rashi Kochhar (1), Shailendra Rane (1), Shailesh Damale

More information

Determination of Table 3 Compounds by LC/MS/MS Chemours Fluoroproducts Analytical Method Revision date: 4/26/2018

Determination of Table 3 Compounds by LC/MS/MS Chemours Fluoroproducts Analytical Method Revision date: 4/26/2018 Determination of Table 3 Compounds by LC/MS/MS Chemours Fluoroproducts Analytical Method Revision date: 4/26/2018 Instrument Setup (Note: Trapping column installed on Line A. Delay column installed between

More information

Accelerating Throughput for Targeted Quantitation of Proteins/Peptides in Biological Samples

Accelerating Throughput for Targeted Quantitation of Proteins/Peptides in Biological Samples Technical Note Accelerating Throughput for Targeted Quantitation of Proteins/Peptides in Biological Samples Biomarker Verification Studies using the QTRAP 5500 Systems and the Reagents Triplex Sahana Mollah,

More information

Tabisam Khan 1, David R. Little 1, Liyu Yang 2, Jiwen Chen 2, Parya Nouri 2, Samy Tadros 2, Patrick J. Rudewicz 2

Tabisam Khan 1, David R. Little 1, Liyu Yang 2, Jiwen Chen 2, Parya Nouri 2, Samy Tadros 2, Patrick J. Rudewicz 2 AIMS High throughput bioanalysis plays a central role in the drug discovery and development process. The resulting data are used to determine a drug candidate's pharmacokinetic parameters that are crucial

More information

HARMONIZATION TEAM A6 (STABILITY) UPDATE. Yoshiaki Ohtsu 8 March 2012

HARMONIZATION TEAM A6 (STABILITY) UPDATE. Yoshiaki Ohtsu 8 March 2012 HARMONIZATION TEAM A6 (STABILITY) UPDATE Yoshiaki Ohtsu 8 March 2012 Contents 1. General update 2. Scientific discussion point 1. Stability of presence of co administered or co formulated drugs 2. Incurred

More information

Separation of Recombinant Human Erythropoietin (rhepo) using the European Pharmacopoeia Method on the PA 800 plus Pharmaceutical Analysis System

Separation of Recombinant Human Erythropoietin (rhepo) using the European Pharmacopoeia Method on the PA 800 plus Pharmaceutical Analysis System Separation of Recombinant Human Erythropoietin (rhepo) using the European Pharmacopoeia Method on the PA 800 plus Pharmaceutical Analysis System Marcia R Santos Global Tactical Marketing, Beckman Coulter

More information

More Samples. Better Decisions Faster.

More Samples. Better Decisions Faster. More Samples. Better Decisions Faster. High Throughput Glycan Screening and Identification C100HT Biologics Analyzer Screening large sample cohorts is challenging in many ways. The neutral charge of glycans

More information

Simplifying ImmunoAffinity Capture Workflow

Simplifying ImmunoAffinity Capture Workflow CAE TUDY MART Digest ImmunoAffinity (IA) Kit implifying ImmunoAffinity Capture Workflow Rapid, ensitive, LC-RM Quantitative Analysis of Proteins in Plasma As pharmaceuticals grow more efficacious, reporting

More information

Biotherapeutic Peptide Mapping Information Dependent Acquisition (IDA) Method

Biotherapeutic Peptide Mapping Information Dependent Acquisition (IDA) Method Biotherapeutic Peptide Mapping Information Dependent Acquisition (IDA) Method Routine biotherapeutic accurate mass peptide mapping analysis on the X500B QTOF System Method details for the routine peptide

More information

Rapid Soft Spot Analysis using the SCIEX Routine Biotransform Solution

Rapid Soft Spot Analysis using the SCIEX Routine Biotransform Solution Rapid Soft Spot Analysis using the SCIEX Routine Biotransform Solution Rapidly Identify Top Metabolites with the SCIEX X500R QTOF System and MetabolitePilot TM 2.0 Software Shaokun Pang and Ian Moore SCIEX,

More information

A Rapid and Reproducible Immuno-MS Platform from Sample Collection to Quantitation of IgG

A Rapid and Reproducible Immuno-MS Platform from Sample Collection to Quantitation of IgG PO-CON1457E A Rapid and Reproducible Immuno-MS Platform from Sample Collection to Quantitation of IgG ASMS 14 ThP766 Rachel Lieberman 1, David Colquhoun 1, Jeremy Post 1, Brian Feild 1, Scott Kuzdzal 1,

More information

Analysis of Intact Monoclonal Antibodies Using an M3 MicroLC with the TripleTOF 6600

Analysis of Intact Monoclonal Antibodies Using an M3 MicroLC with the TripleTOF 6600 Procedures Analysis of Intact Monoclonal Antibodies Using an M3 MicroLC with the TripleTOF 66 Robust and Sensitive Workflow for Qualitative and Quantitative Analysis of Biotherapeutic IgGs Khatereh Motamedchaboki,

More information

BioBA High Capacity Enrichment Sample Preparation Kit:

BioBA High Capacity Enrichment Sample Preparation Kit: BioBA High Capacity Enrichment Sample Preparation Kit: Biologics Bioanalysis Made Easy Ian Moore 1, Chang Liu 1, Gary Impey 1, Suma Ramagiri 1 1 Sciex, Concord, Canada Common Immunocapture Sample Extraction

More information

Updating Solid Phase Extraction Methods: Tips and Tricks for Improving Existing and New SPE Methods using Method Development

Updating Solid Phase Extraction Methods: Tips and Tricks for Improving Existing and New SPE Methods using Method Development Updating Solid Phase Extraction Methods: Tips and Tricks for Improving Existing and New SPE Methods using Method Development Golnar Javadi Application Engineer Sample Preparation Products Spp-support@agilent.com

More information

Bioanalytical Concentrations Below the LLOQ:

Bioanalytical Concentrations Below the LLOQ: Bioanalytical Concentrations Below the LLOQ: Should they be reported? arguments against What are regulatory and technical issues? What should we do to verify a lower LLOQ? Jeff Duggan and Jim Hilbert Boehringer

More information

High-Throughput Glycan Screening to Remove Biopharmaceutical Development Bottlenecks

High-Throughput Glycan Screening to Remove Biopharmaceutical Development Bottlenecks High-Throughput Glycan Screening to Remove Biopharmaceutical Development Bottlenecks Mark Lies, Marcia Santos, and Tingting Li SCIEX Separations, Brea, CA Introduction Development of biopharmaceuticals

More information

A TFC MS/MS Method for the Determination of the Cholinesterase Sensitive Analyte Rivastigmine in Human Plasma

A TFC MS/MS Method for the Determination of the Cholinesterase Sensitive Analyte Rivastigmine in Human Plasma White Paper A TFC MS/MS Method for the Determination of the Cholinesterase Sensitive Analyte Rivastigmine in Human Plasma Lars Neudert, MSc, Senior Scientist Method Development Laurence Meunier, PhD, Senior

More information

Biotherapeutic Non-Reduced Peptide Mapping

Biotherapeutic Non-Reduced Peptide Mapping Biotherapeutic Non-Reduced Peptide Mapping Routine non-reduced peptide mapping of biotherapeutics on the X500B QTOF System Method details for the routine non-reduced peptide mapping of a biotherapeutic

More information

Improved SPE for UPLC/MS Determination of Diquat and Paraquat in Environmental

Improved SPE for UPLC/MS Determination of Diquat and Paraquat in Environmental Improved SPE for UPLC/MS Determination of Diquat and Paraquat in Environmental Samples Michael S.Young, Jeremy C. Shia, Kim vantran, Kevin M. Jenkins and Masayo Yabo Waters Corporation 34 Maple Street,

More information

Isotopic Resolution of Chromatographically Separated IdeS Subunits Using the X500B QTOF System

Isotopic Resolution of Chromatographically Separated IdeS Subunits Using the X500B QTOF System Isotopic Resolution of Chromatographically Separated IdeS Subunits Using the X500B QTOF System Fan Zhang 2, Sean McCarthy 1 1 SCIEX, MA, USA, 2 SCIEX, CA USA Analysis of protein subunits using high resolution

More information

Above and beyond the extraordinary

Above and beyond the extraordinary AB SCIEX QTRAP 5500 System Above and beyond the extraordinary Above and beyond the competition speed and sensitivity that solve problems other systems cannot Powerful synergy results when the world s most

More information

Pesticide Residues in Produce Analyzed by Targeted MRM HR Full- Scan Acquisition and Processing

Pesticide Residues in Produce Analyzed by Targeted MRM HR Full- Scan Acquisition and Processing Pesticide Residues in Produce Analyzed by Targeted MRM HR Full- Scan Acquisition and Processing MRM HR for Concurrent Quantitation, Library Searching, and High-Confidence ID Confirmation KC Hyland SCIEX,

More information

Strategies for Phospholipid Removal using Polymer-based SPE

Strategies for Phospholipid Removal using Polymer-based SPE Strategies for Phospholipid emoval using Polymer-based SPE Lee Williams, Helen Lodder, Matthew Cleeve, Scott Merriman, Steve Jordan, ichard Calverley, Steve Plant, Joanna Smith Introduction Sample preparation

More information

Q40. This automated platform will streamline the two part QuEChERS method from the liquid extraction through the cleanup.

Q40. This automated platform will streamline the two part QuEChERS method from the liquid extraction through the cleanup. Application Note Abstract With the rise in popularity of QuEChERS, due to its reliable multiresidue methods it has driven the need for automation of the QuEChERS extraction to increase productivity and

More information

A Rapid imethod TM Test for Analysis of Four Immunosuppressants

A Rapid imethod TM Test for Analysis of Four Immunosuppressants imethod Test A Rapid imethod TM Test for Analysis of Four Immunosuppressants The following description outlines the instrument requirements and expected results obtainable from the Spark Holland imethod

More information

Quantitation of cyanotoxins in drinking water according to EPA 544 guidelines

Quantitation of cyanotoxins in drinking water according to EPA 544 guidelines APPLICATION NOTE 64968 Quantitation of cyanotoxins in drinking water according to EPA 544 guidelines Authors Ali Haghani, 1 Andy Eaton, 1 Neloni Wijeratne, Claudia Martins 1 Eurofins Eaton Analytical,

More information

Application Note. Abstract. Authors. Food Safety

Application Note. Abstract. Authors. Food Safety Determination of Chloramphenicol, Florfenicol, and Thiamphenicol in Honey Using Agilent SampliQ PT Solid-Phase Extraction Cartridges and Liquid Chromatography-Tandem Mass Spectrometry Application Note

More information

Monitoring for Microcystins in Raw Water Supply Reservoirs Using the Agilent 6410 Triple Quadrupole LC/MS

Monitoring for Microcystins in Raw Water Supply Reservoirs Using the Agilent 6410 Triple Quadrupole LC/MS Monitoring for Microcystins in Raw Water Supply Reservoirs Using the Agilent 641 Triple Quadrupole LC/MS Application Note Environmental Author Toni Hall Wessex Water Bath, UK Abstract A method for the

More information

APPLICATION NOTE. Abstract. Introduction

APPLICATION NOTE. Abstract. Introduction APPLICATION NOTE Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS SCIEX Triple Quad 4500 LC/MS/MS system and Beckman Coulter Biomek 4000 Workstation Lekh

More information

CLSI C60: Assay Validation & Post-Validation Monitoring

CLSI C60: Assay Validation & Post-Validation Monitoring CLSI C60: Assay Validation & Post-Validation Monitoring Ross J. Molinaro, MT(ASCP), PhD, DABCC, FACB Medical Director Core Laboratory, Emory University Hospital Midtown Emory Clinical Translational Research

More information

A fast and simple workflow for surrogate peptide bioanalysis: NISTmAb case study

A fast and simple workflow for surrogate peptide bioanalysis: NISTmAb case study APPLICATION NOTE 21767 A fast and simple workflow for surrogate peptide bioanalysis: NISTmAb case study Authors Jon Bardsley, Thermo Fisher Scientific, Runcorn, UK Keywords Biopharmaceutical, bioanalytical,

More information

Quantification of genotoxic "Impurity D" in Atenolol by LC/ESI/MS/MS with Agilent 1200 Series RRLC and 6410B Triple Quadrupole LC/MS

Quantification of genotoxic Impurity D in Atenolol by LC/ESI/MS/MS with Agilent 1200 Series RRLC and 6410B Triple Quadrupole LC/MS Quantification of genotoxic "Impurity D" in Atenolol by LC/ESI/MS/MS with Agilent 12 Series RRLC and 641B Triple Quadrupole LC/MS Application Note Manufacturing Process Development Author Siji Joseph Agilent

More information

UPLC/MS/MS Method for the Quantification of Trastuzumab in Human Serum at the 5-nM Level Using Xevo TQD MS and ACQUITY UPLC H-Class System

UPLC/MS/MS Method for the Quantification of Trastuzumab in Human Serum at the 5-nM Level Using Xevo TQD MS and ACQUITY UPLC H-Class System UPLC/MS/MS Method for the Quantification of Trastuzumab in Human Serum at the 5-nM Level Using Xevo TQD MS and ACQUITY UPLC H-Class System Catalin Doneanu and Robert Plumb Waters Corporation, Milford,

More information

AB SCIEX 4500 SERIES MASS SPECTROMETERS. The new workhorse for LC/MS/MS AB SCIEX 4500 SERIES MASS SPECTROMETERS

AB SCIEX 4500 SERIES MASS SPECTROMETERS. The new workhorse for LC/MS/MS AB SCIEX 4500 SERIES MASS SPECTROMETERS AB SCIEX 4500 SERIES MASS SPECTROMETERS The new workhorse for LC/MS/MS AB SCIEX 4500 SERIES MASS SPECTROMETERS The new AB SCIEX 4500 Series takes the legendary API 4000 platform and intelligently re-engineers

More information

MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics

MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics Product Bulletin MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics With Unique QTRAP System Technology Overview Targeted peptide quantitation is a rapidly growing

More information

A Generic Approach to the Extraction of Multi-functional Drugs using Mixed-mode SPE with LC-MS/MS Analysis

A Generic Approach to the Extraction of Multi-functional Drugs using Mixed-mode SPE with LC-MS/MS Analysis A Generic Approach to the Extraction of Multi-functional Drugs using Mixed-mode SPE with LC-MS/MS Analysis Matthew Cleeve, Scott Merriman, Lee Williams, Steve Jordan, Richard Calverley, Joanna Smith &

More information

Evaluation by Competent Authorities

Evaluation by Competent Authorities LANXESS Deutschland GmbH Chlorophene 07/2007 ACTIVE SUBSTANCE RESIDUES IN ANIMAL AND HUMAN BODY FLUIDS AND TISSUES JUSTIFICATION FOR NON-SUBMISSION OF DATA Official use only Other existing data [ ] Technically

More information

Size Exclusion Chromatography of Biosimilar and Innovator Insulin Using the Agilent AdvanceBio SEC column

Size Exclusion Chromatography of Biosimilar and Innovator Insulin Using the Agilent AdvanceBio SEC column Size Exclusion Chromatography of Biosimilar and Innovator Insulin Using the Agilent AdvanceBio SEC column Application Note Bio-Pharmaceutical Authors M. Sundaram Palaniswamy and Andrew Coffey Agilent Technologies,

More information

Practical Aspects of Dried Blood Spot (DBS) Bioanalytical Assay Development and Application. Qin C. Ji CASSS, 09 September 2010

Practical Aspects of Dried Blood Spot (DBS) Bioanalytical Assay Development and Application. Qin C. Ji CASSS, 09 September 2010 Practical Aspects of Dried Blood Spot (DBS) Bioanalytical Assay Development and Application Qin C. Ji CASSS, 09 September 2010 1 Outline Introduction Potential advantages of DBS DBS assay development Result

More information

A Novel Dried Matrix Microsampling. Eliminates the Volume Based Hematocrit Bias Associated with DBS Sub-punch Workflows

A Novel Dried Matrix Microsampling. Eliminates the Volume Based Hematocrit Bias Associated with DBS Sub-punch Workflows A Novel Dried Matrix Microsampling Device that Eliminates the Volume Based Hematocrit Bias Associated with DBS Sub-punch Workflows Stuart Kushon1; Allen Bischofberger1; Anna Carpenter1; Philip Denniff2;

More information

Monitoring for Microcystins in Raw Water Supply Reservoirs Using the Agilent 6410 Triple Quadrupole LC/MS

Monitoring for Microcystins in Raw Water Supply Reservoirs Using the Agilent 6410 Triple Quadrupole LC/MS Monitoring for Microcystins in Raw Water Supply Reservoirs Using the Agilent 641 Triple Quadrupole LC/MS Application Note Environmental Author Toni Hall Wessex Water Bath, UK Abstract A method for the

More information

MasterView Software. Turn data into answers reliable data processing made easy ONE TOUCH PRODUCTIVITY

MasterView Software. Turn data into answers reliable data processing made easy ONE TOUCH PRODUCTIVITY MasterView Software Turn data into answers reliable data processing made easy ONE TOUCH PRODUCTIVITY Mass spectrometers especially high-resolution, accurate mass instruments produce a significant amount

More information

New Approaches to Quantitative Proteomics Analysis

New Approaches to Quantitative Proteomics Analysis New Approaches to Quantitative Proteomics Analysis Chris Hodgkins, Market Development Manager, SCIEX ANZ 2 nd November, 2017 Who is SCIEX? Founded by Dr. Barry French & others: University of Toronto Introduced

More information

The Agilent Metabolomics Dynamic MRM Database and Method

The Agilent Metabolomics Dynamic MRM Database and Method The Agilent Metabolomics Dynamic MRM Database and Method White Paper Author Mark Sartain Agilent Technologies, Inc. Santa Clara, California, USA Introduction A major challenge in metabolomics is achieving

More information

ProteinPilot Report for ProteinPilot Software

ProteinPilot Report for ProteinPilot Software ProteinPilot Report for ProteinPilot Software Detailed Analysis of Protein Identification / Quantitation Results Automatically Sean L Seymour, Christie Hunter SCIEX, USA Powerful mass spectrometers like

More information

Protocol for Quantitative Determination of Residual Solvents in Cannabis Concentrates Prepared by: Amanda Rigdon, May 23 rd, 2016

Protocol for Quantitative Determination of Residual Solvents in Cannabis Concentrates Prepared by: Amanda Rigdon, May 23 rd, 2016 Protocol for Quantitative Determination of Residual Solvents in Cannabis Concentrates Prepared by: Amanda Rigdon, May 23 rd, 2016 1.0 Method background: This method employs full evaporation technique headspace

More information

Biologics Bioanalysis

Biologics Bioanalysis Biologics Bioanalysis GUIDE TO INNOVATION Biologics Bioanalysis Guide to Innovation Pharmaceutical companies have leveraged advancements in basic science perhaps more than any other industry. With the

More information

EPA Method 540: Selected Organic Contaminants Using Agilent Plexa Cartridges and the Agilent 6460 Triple Quadrupole LC/MS

EPA Method 540: Selected Organic Contaminants Using Agilent Plexa Cartridges and the Agilent 6460 Triple Quadrupole LC/MS EPA Method 540: Selected Organic Contaminants Using Agilent Plexa Cartridges and the Agilent 6460 Triple Quadrupole LC/MS Application Note Environmental Authors Don Noot and Ralph Hindle Vogon Laboratory

More information

API 3200 LC/MS/MS system

API 3200 LC/MS/MS system www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment API 3200 LC/MS/MS system Reliability, Reproducibility, and Confidence in Your

More information

Development and evaluation of Nano-ESI coupled to a triple quadrupole mass spectrometer for quantitative proteomics research

Development and evaluation of Nano-ESI coupled to a triple quadrupole mass spectrometer for quantitative proteomics research PO-CON138E Development and evaluation of Nano-ESI coupled to a triple quadrupole mass spectrometer for quantitative proteomics research ASMS 213 ThP 115 Shannon L. Cook 1, Hideki Yamamoto 2, Tairo Ogura

More information