Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK The Cefas Weymouth Laboratory The Nothe Barrack Road Weymouth DT4 8UB Contact: Miss D Page Tel: +44 (0) Fax: +44 (0) debbie.page@cefas.co.uk Website: Testing performed at the above address only DETAIL OF ACCREDITATION - BIVALVE MOLLUSCS Mussels, cockles, Pacific oysters, native oysters, scallops (whole king, whole queen and processed king) and clams (razor, hard, surf, manila, otter and carpet shell) pre-shucked king scallops (adductor only and adductor roe) Mussels, cockles, Pacific Oysters, Native oysters, scallops (whole King, whole queen and processed king) and clams (razor, surf, manila, otter and hard) Fresh water, algae, blue-green algae food supplements (tablets or powder) and shell fish Chemical Analysis Detection of Domoic acid (amnesic shellfish poisoning toxin, ASP), Paralytic shellfish poisoning toxin (PSP): Semi-quantitative screening and quantitative test Lipophilic Marine Toxins (DSP) : Quantitative test Determination of Cyanotoxins: Quantitative test MC-RR MC-LA MC-LY MC-LF MC-LW MC-YR MC-WR MC-HilR MC-HtyR MC-LR Asp 3 -MC-LR Asp 3 -MC-RR Nodularin Documented in-house methods: SOPs 1180, 1744, 1200, 1243 and 1723, by HPLC-UV SOPs 1180, 1744, 1784, 1785, 1788 and 2168 by HPLC-FLD based on AOAC Official method SOPs 1180, , 2099 and 2101 by LC-MS/MS SOP 2239 by LC-MS/MS Assessment Manager: KH Page 1 of 5
2 Bivalve mollusc digestive gland Bivalve Mollusc Digestive Gland Crustacean Tissue Molecular Biology Quantification of norovirus genogroup I and II RNA Quantification of Hepatitis A virus RNA white spot syndrome virus (WSSV) DNA (absence or Cyprinid Herpes virus type III (CyHV-3) DNA (absence or Spring Viraemia of Carp virus (SVCV) RNA (absence or SOP 1330 by real-time reverse transcriptase PCR using Mx3005P Stratagene thermocycler SOP 1330 using real-time RT-PCR based on ISO :2017 by TaqMan chemistry using the Stratagene Mx3005P thermal cycler SOP 2076 by robotic extraction (Qiagen BioRobot EZ1 or EZ1 advanced XL) with Qiagen DNA tissue kit followed by conventional PCR as stated in the OIE manual of Diagnostic Tests for Aquatic Animals. Products direct sequenced using the ABI Bg Dye Terminator Kit v3.1 and ABI 3500xl genetic analyser SOP 2152 Prepared by robotic a published PCR method. Products direct sequenced using the ABI Big SOPs 2063, Prepared by robotic EZ1 Advanced XL) with Qiagen conventional PCR as stated in the OIE manual of Diagnostic Tests for Aquatic Animals. Products direct sequenced using the ABI Big Dye Terminator kit v3.1 Assessment Manager: KH Page 2 of 5
3 Fish tissues Fish tissues Crustacean tissues Molecular Biology (cont d) Koi Herpes virus (KHV) DNA (absence or Viral Haemorrhagic septicaemia virus (VHSV) RNA (absence or Infectious Haematopoietic Necrosis virus (IHNV) RNA (absence or Yellow Head Virus (YHV) RNA (absence or SOPs Prepared by robotic conventional PCR as stated in the OIE manual of Diagnostic Tests for Aquatic Animals. Followed by a nested PCR designed in house. Products direct sequencing using the ABI Big SOP 2108 Prepared by robotic published conventional RT-PCR methods. Products direct sequencing using the ABI Big SOP Prepared by robotic conventional RT-PCR as stated in the OIE manual of Diagnostic Tests for Aquatic Animals. Products direct sequencing using the ABI Big SOP Prepared by robotic RNA Tissue kit, followed by conventional RT-PCR as stated in the OIE manual of Diagnostic Tests for Aquatic Animals. Products direct sequencing using the ABI Big Assessment Manager: KH Page 3 of 5
4 Fish, Shellfish and Crustacean tissue Bivalve Molluscs Molecular Biology (cont d) Detection and identification of viruses, bacteria, fungi and parasites Microbiological Tests Enumeration: Escherichia coli Detection: Salmonella spp (excluding S. typhi) SOPs 2008, 2006, 2007,1537, 1742,1546 by robotic extraction, conventional PCR including RT- PCR and sequence analysis using ABI 3130 analyser under Flexible Scope SOP 2064 SOP 1175, based on ISO/TS :2005 SOP 1176 Vibrio parahaemolyticus SOP 1333 Brain, Kidney and Spleen Testing Virological Tests Isolation and identification of Viral Haemorrhagic Septicaemia virus (VHSV) Isolation and identification of Infectious Haematopoietic Necrosis virus (IHNV) SOP s 1248, 1279, 1125 and 1259 by tissue culture and ELISA in accordance with Commission Decision (EU) 2015/1554, Council SOP s 1248, 1279, 1125 and 1263 by tissue culture and IFAT in accordance with Commission Decision (EU) 2015/1554, Council Assessment Manager: KH Page 4 of 5
5 FISH, Bivalve Mollusc and Crustacean tissues Brain, Kidney and Spleen Fish tissues (homogenate supernatants) Fish, shellfish, crustacean tissues Fish, shellfish, crustacean tissues Fish tissue and homogenates Virological Tests (cont d) Isolation and identification of spring Viraemia of Carp (SVC) virus Isolation of Epizootic haematopoietic necrosis virus (EHNV) Detection and Identification of Spring Viraemia of Carp virus (SVCV) RNA Detection and Identification of Koi Herpes virus (KHV) DNA Isolation and identification of Infectious Salmon Anaemia virus (ISAV) Parasitology Tests SOP s 1248, 1279, 1125 and 1259 by tissue culture and ELISA in accordance with Council Directive 2006/88/EC (based on OIE Manual of Diagnostic Tests for Aquatic Animals, 2009) SOP s 1248, 1279 and 1125 by tissue culture (BF2 and EPC cells) in accordance with Council SOPs 2063,1245, 2008, 2006,2007, 1537, 1742 and 1546 by RT-PCR. Guidance from the Aquatic Animal Health Directive - Council Directive 2006/88 (based on OIE Manual of Diagnostic tests for Aquatic Animals 2006) SOPs 1493,1245, 2008, 2006,2007, 1742 and 1546 by PCR. Guidance from the Aquatic Animal Health Directive - Council Directive 2006/88 (based on OIE Manual of Diagnostic tests for Aquatic Animals 2006) SOPs 1248, 1279, 1125,1263 & 1268 by tissue culture (TO and SHK cells) and IFAT in accordance with Commission Decision (EU) 2015/1554, Council Directive 2006/88/EC (based on OIE Manual of Diagnostic Tests for Aquatic Animals 2009) Salmonids fins Identification of Gyrodactylus salaris SOP 1735 by light microscopy END Assessment Manager: KH Page 5 of 5
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