Pablo Domínguez de María

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1 Personal Data Name: Date / Place of birth: Pablo Domínguez de María Las Palmas de Gran Canaria. Spain. CURRICULUM VITAE Educational Background Ph.D. in Pharmacy with European Mention, presented both in English and Spanish. Organic & Pharmaceutical Chemistry Department. Faculty of Pharmacy. Universidad Complutense de Madrid. Spain MSc in Pharmacy. Organic & Pharmaceutical Chemistry Department. Faculty of Pharmacy. Universidad Complutense de Madrid. Spain BSc in Pharmacy. Universidad Complutense de Madrid. Spain. Final average score 8.6 (out of 10) BSc in Chemistry. Open University of Spain (UNED). Research positions / Work Experience Jul 2009 Onwards. R&D Group Leader (Biotransformations, Bio-mimetic organocatalytic concepts, and lignocellulose processing). Institute of Technical and Macromolecular Chemistry (ITMC). University of Aachen (RWTH Aachen). Germany. Feb 2005 Jun AkzoNobel BV. Process & Product Technology Department (Central Research Center). Arnhem. The Netherlands. Several positions: Feb 2007 Jun R&D Project Leader. Biotransformations, Organocatalysis, Sustainable Chemistry. Duties: Assessment of industrially feasible (bio-) & (organo- )catalytic processes; new trends concerning White Biotechnology. Feb 2005 Jan Postdoctoral Research Scientist. Program EU Transfer of Knowledge. Project acquisition from Business Units (Biocatalysis) and implementation. Feb 2003 Jan Postdoctoral Research Scientist EU Marie Curie. Degussa AG (Evonik), within Biotechnology Project House (2003) and Service Center Biocatalysis (2004). Hanau-Wolfgang. Germany. R&D of biocatalytic processes for their industrial implementation. Oct 1998 Oct Ph.D. Student. Organic & Pharmaceutical Chemistry Department. Biotransformations group. Faculty of Pharmacy. Universidad Complutense de Madrid ( Spain.

2 May Jun 2000 & May 2001: Pre-doctoral stays. National Hellenic Research Foundation. Institute of Biological Research & Biotechnology. Athens, (Greece) ( Jun 1997 Oct MSc Student. Organic & Pharmaceutical Chemistry Department. Universidad Complutense de Madrid. (Field: Biocatalysis & Biotransformations). Jul 1996 May Internship. Organic & Pharmaceutical Chemistry Department. Universidad Complutense de Madrid. (Field: Biocatalysis & Biotransformations). Languages Spanish: Mother tongue English: Full written and oral command. First Certificate (Cambridge University, 1992). German: Written and oral command. Dutch: Basic knowledge. Awards Award Young Scientist Ibero-American Academy of Pharmacy (2005). Ph.D. Award Premio de Doctorado Abilio Rodríguez Paredes. Faculty of Pharmacy. Universidad Complutense de Madrid. Academic course Award Premio Extraordinario de Bachillerato for pre-university studies, given by the Regional Government of Canary Islands. ( ). Patent Applications 2.- Domínguez de María, P., Trauthwein, H., May, O., Gröger, H., Drauz, K. Verfahren zur Herstellung von enantiomer angereicherten alpha-hydroxyketonen. DE (2005). Process for preparing enantiomerically enriched α-hydroxyketones. WO (A1). 1.- Wallert, S., Grayson, I., Drauz, K., Gröger, H., Domínguez de María, P., Chamouleau, F., Bolm, C. Enzymatische Reaktionen in Gegenwart ionischer Additive. DE Enzymatic reactions in the presence of ionic additives. WO (A1).

3 Peer-reviewed Scientific Publications (*) as corresponding author Domínguez de María, P.*, Bracco, P., Castelhano, L.F., Bargeman, G. Influence of the organocatalyst in the aldol / Mannich-type product selectivities in C-C bond forming reactions. ACS Catalysis. 2011, 1, Domínguez de María, P.*. Recent developments in the biotechnological production of hydrocarbons: Paving the way for bio-based platform chemicals. ChemSusChem Accepted for publication Domínguez de María, P.*, Maugeri, Z. Ionic Liquids in Biotransformations: From proof-of-concept to emerging deep-eutectic-solvents. Current Opinion in Chemical Biology Accepted for publication Domínguez de María, P.*, Shanmuganathan, S. Umpolung catalysis in Benzoin-type and Stetter-type reactions: From enzymatic performances to bio-mimetic organocatalytic concepts. Current Organic Chemistry Accepted for publication Shanmuganathan, S., Natalia, D., van den Wittenboer, A., Kohlmann, C., Greiner, L.*, Domínguez de María, P.* Enzyme-catalyzed C-C bond formation using 2- methyltetrahydrofuran (2-MTHF) as (co)solvent: Efficient and bio-based alternative to DMSO and MTBE. Green Chemistry. 2010, 12, Shanmuganathan, S., Greiner, L., Domínguez de María, P.* Silica-immobilized piperazine: A sustainable organocatalyst for aldol and Knoevenagel reactions. Tetrahedron Letters. 2010, 51, vom Stein, T., Grande, P., Sibilla, F., Commandeur, U., Fischer, R., Leitner, W., Domínguez de María, P.* Salt-assisted organic acid-catalyzed depolymerization of cellulose. Green Chemistry. 2010, 12, Domínguez de María, P.* Minimal Hydrolases: Organocatalytic ring-opening polymerizations catalyzed by naturally-occurring carboxylic acids. ChemCatChem. 2010, 2, Domínguez de María, P.*, van Gemert, R.W., Straathof, A.J.J., Hanefeld, U.* "Biosynthesis of ethers: Unusual or common natural events?". Natural Products Reports. 2010, 27,

4 27.- Hoyos, P., Sinisterra, J.V., Molinari, F., Alcántara, A.R.*, Domínguez de María, P.* "Biocatalytic strategies for the asymmetric synthesis of α-hydroxy ketones". Accounts of Chemical Research, 2010, 43, Gamenara, D.*, Domínguez de María, P*. "Candida spp. redox machineries: An ample biocatalytic platform for practical applications and academic insights". Biotechnology Advances, 2009, 27, Domínguez de María, P.*, Fernández-Álvaro, E., ten Kate, A., Bargeman, G. "Role of apparent pk a of carboxylic acids in lipase-catalyzed esterifications in biphasic systems". Journal of Molecular Catalysis B: Enzymatic. 2009, 59, Domínguez de María, P.*, Martinsson, A. "Ionic Liquid-based method to determine the degree of esterification in cellulose fibers". The Analyst, 2009, 134, Domínguez de María, P.* Nonsolvent applications of Ionic Liquids (RTILs) in Biotransformations and Organocatalysis. Angewandte Chemie International Edition. 2008, 47, ; "Nonsolvents - Anwendungen von ionischen Flüssigkeiten bei Biotransformationen und in der Organokatalyse". Angewandte Chemie, 2008, 120, Beloqui, A., Domínguez de María, P., Golyshin, P., Ferrer, M. "Recent trends in industrial microbiology". Current Opinion in Microbiology, 2008, 11, Zoumpanioti, M., Parmaklis, P., Domínguez de María, P., Stamatis, H., Sinisterra, J.V., Xenakis, A. Esterification and reactions catalyzed by lipases immobilized in organogels. Effect of temperature and substrate diffusion. Biotechnology Letters, 2008, 30, Kourist, R., Domínguez de María, P., Bornscheuer, U.T. Enzymatic synthesis of optically active tertiary alcohols: Expanding the biocatalysis toolbox. ChemBioChem, 2008, 9, Domínguez de María, P., Stillger, T., Pohl, M., Kiessel, M., Liese, A., Gröger, H., Trauthwein, H. Enantioselective C-C bond ligation using recombinant E.coli Whole Cell biocatalysts. Advanced Synthesis & Catalysis, 2008, 350, Domínguez de María, P.*, García-Burgos, C.A., Bargeman, G., van Gemert, R.W. Pig Liver Esterase (PLE) as biocatalyst in organic synthesis: From nature to cloning and to practical applications. Synthesis, 2007, 10, Domínguez de María, P., Pohl, M., Gocke, D., Gröger, H., Trauthwein, H., Stillger, T., Wallter, L., Müller, M. Asymmetric synthesis of aliphatic 2-hydroxy-ketones by enzymatic carboligation of aldehydes. European Journal of Organic Chemistry. 2007, 18,

5 Carboni-Oerlemans, C.*, Domínguez de María, P.*, Tuin, B., Bargeman, G., van der Meer, A.B., van Gemert, R.W. Hydrolase-catalysed synthesis of peroxycarboxylic acids: Biocatalytic promiscuity for practical applications. Journal of Biotechnology, 2006, 126, Domínguez de María, P., Sinisterra, J.V., Tsai, S.W., Alcántara, A.R. Carica papaya lipase: an emerging and versatile biocatalyst. Biotechnology Advances, 2006, 24, Domínguez de María, P., Alcántara, A.R., Carballeira, J.D., de la Casa, R.M., García- Burgos, C.A., Hernáiz, M.J., Sánchez-Montero, J.M., Sinisterra, J.V. Candida rugosa lipase: A traditional and complex biocatalyst. Current Organic Chemistry, 2006, 10, Domínguez de María, P., Sinisterra, J.V., Sánchez-Montero, J.M., Lotti, M., Valero, F., Alcántara, A.R. Acyl transfer strategy for the biocatalytical characterisation of Candida rugosa lipases in organic solvents. Enzyme and Microbial Technology, 2006, 38, Domínguez de María, P., Stillger, T., Wallert, S., Pohl, M., Gröger, H., Drauz, K.H., Trauthwein, H., Liese, A. Preparative enantioselective synthesis of benzoins and (R)-2- hydroxy-1-phenylpropanone by using benzaldehyde lyase. Journal of Molecular Catalysis B: Enzymatic. 2006, 38, Domínguez de María, P., Sánchez-Montero, J.M., Sinisterra, J.V., Alcántara, A.R. Understanding Candida rugosa lipases: an overview. Biotechnology Advances, 2006, 24, Domínguez de María, P.*, Carboni-Oerlemans, C.*, Tuin, B., Bargeman, G., van der Meer, A.B., van Gemert, R.W. Biotechnological applications of Candida antarctica lipase A: State-of-the-art. Journal of Molecular Catalysis B: Enzymatic, 2005, 37, Wallert, S., Drauz, K., Grayson, I., Gröger, H., Domínguez de María, P., Bolm, C. Ionic liquids as additives in the pig liver esterase (PLE) catalysed synthesis of chiral disubstituted malonates. Green Chemistry, 2005, 7, Domínguez de María, P., Kossmann, B., Potgrave, N., Buchholz, S., Trauthwein, H., May, O., Gröger, H. Improved process for the enantioselective hydrolysis of prochiral diethyl malonates catalyzed by Pig Liver Esterase. Synlett, 2005, 11, Domínguez de María, P., Sánchez-Montero, J.M., Alcántara, A.R., Valero, F., Sinisterra, J.V. Rational strategy for the production of new crude lipases from Candida rugosa. Biotechnology Letters, 2005, 27,

6 Alcántara, A.R., Domínguez de María, P., Fernández, M., Hernáiz, M.J., Sánchez- Montero, J.M., Sinisterra, J.V. Resolution of racemic acids, esters and amines by Candida rugosa lipase in slightly hydrated organic media. Food Technology Biotechnology, 2004, 42, Domínguez de María, P., Xenakis, A., Stamatis, H., Sinisterra, J.V. Unexpected reaction profile observed in the synthesis of propyl laurate when using Candida rugosa lipases immobilized in microemulsions based organogels. Biotechnology Letters, 2004, 26, Domínguez de María, P., Xenakis, A., Stamatis, H., Sinisterra, J.V. Lipase Factor (LF) as a characterization parameter to explain the catalytic activity of crude lipases from Candida rugosa, free or immobilised in microemulsion-based organogels. Enzyme and Microbial Technology, 2004, 35, Domínguez de María, P., Martínez-Alzamora, F., Pérez Moreno, S., Valero, F., Rúa, M.L., Sánchez-Montero, J.M., Sinisterra, J.V., Alcántara, A.R. Heptyl oleate synthesis as useful tool to discriminate between lipases, proteases and other hydrolases in crude preparations. Enzyme and Microbial Technology, 2002, 31, de Castro, M.S., Domínguez de María, P., Sinisterra, J.V. Enzymatic amidation and alkoxycarbonylation of amines using native and immobilised lipases with different origins: a comparative study. Tetrahedron, 2000, 56, Domínguez de María, P., Sinisterra, J.V. Causes of unreproducibility of C. rugosa lipase-catalyzed reactions in slightly hydrated organic media. Tetrahedron, 1999, 55, Books Domínguez de María, P. (Ed.). Ionic Liquids in Biotransformations & Organocatalysis: Solvents and Beyond. John Wiley & Sons Inc., Hoboken (NJ, USA). Submitted to Editorial. Publication expected in Scientific Contributions to Book Chapters 5.- Domínguez de María, P., Kohlmann, C. Non-solvent applications of ionic liquids in biotransformations. In "Ionic Liquids in Biotransformations & Organocatalysis: Solvents and Beyond. Dr. Pablo Domínguez de María (Ed). John Wiley & Sons Inc., Hoboken, (NJ, USA) In press.

7 4.- Gamenara, D., Sáenz-Méndez, P., Seoane, G. Domínguez de María, P. Ionic liquids as (co-)solvents for non-hydrolytic enzymes. In "Ionic Liquids in Biotransformations & Organocatalysis: Solvents and Beyond. Dr. Pablo Domínguez de María (Ed). John Wiley & Sons Inc., Hoboken, (NJ, USA) In press. 3.- Domínguez de María, P. Ionic liquids: Definition, applications and context for biotransformations and organocatalysis. In "Ionic Liquids in Biotransformations & Organocatalysis: Solvents and Beyond. Dr. Pablo Domínguez de María (Ed). John Wiley & Sons Inc., Hoboken, (NJ, USA) In press. 2.- Domínguez de María, P. Procesos industriales biocatalizados. In "Fundamentos de Biocatálisis. Nuevos enfoques en Ciencia y Tecnología". Dr. Elizabeth Lewkowicz (Ed). Universidad Nacional de Quilmes (UNQ). Argentina In press. (In spanish). 1.- Hoyos, P., Sinisterra, J.V., Domínguez de María, P., Alcántara, A.R. Hydrolase-based synthesis of enantiopure α-hydroxy-ketones: From racemic resolutions to chemo-enzymatic dynamic kinetic resolutions. In "Biotechnology: Research, Technology and Applications". Nova Science Publishers, Inc. NY, USA. 2009, pp (Chapter 4). Lectures & Seminars (Invited) 2010 Onwards: Regular presentations in industries (undisclosed). December th regional meeting Biocatalysis & Biotransformations. Montevideo (Uruguay). Plenary Conference: Benzaldehyde Lyase (BAL)-catalyzed reactions: From the academic curiosity to robust and sustainable processes May Symposium: Industrial environment of Biocatalysis and sustainable development. Universidad Nacional de Quilmes. Buenos Aires (Argentina). Title of the lecture: Industrial Biocatalysis. May Symposium: Industrial environment of Biocatalysis and sustainable development. Universidad Nacional de Quilmes. Buenos Aires (Argentina). Title of the lecture: Minimal Biocatalysis: Thinking out-of-the-box. Feb Master on Sustainable Chemistry. Universidad Jaume I. Castellón. Spain. Title of the Lecture: Integrating Biocatalysis at Industrial Scale. Sep th Green Chemistry Conference. Zaragoza, Spain. Title of the Lecture: "Industrial C-C bond forming reactions by means of Bio- and Organocatalysis". Jul Course: "Stereoselective Biocatalysis. Applications in Organic Synthesis". Faculty of Chemistry. Universidad de la República. Montevideo (Uruguay). Implementation of a Brainstorm Session on market-oriented practical applications of Biocatalysis. Jul Course: "Stereoselective Biocatalysis. Applications in Organic Synthesis". Faculty of Chemistry. Universidad de la República. Montevideo (Uruguay). Title of the lecture: "Biotransformations at Industrial Scale".

8 (Technical) Skills and Competences (A) Biocatalysis & Biotransformations. Hydrolase-based reactions (hydrolysis & synthesis) in aqueous and organic media. Enzymatic (enantioselective) C-C bond formation (Lyases). Haloperoxidase-based processes. Nitrile-converting enzymes (Nitrilases and Nitrile-hydratases). Pyridoxal-phosphate-dependent enzymes (transaminases, decarboxylases, etc). Redox enzymes. Enzyme screening. Characterization of biocatalysts. Whole Cells processes. Enzyme & Cell Immobilization (adsorption, covalent union, entrapment...). Mono-, biphasic-, and solvent-free systems. Ionic Liquids as (co-)solvents and additives in biotransformations. Deep-eutectic-solvents (DES) as (co-)solvents and additives in biotransformations. (B) Organic Synthesis & Analytics. HPLC/GC. Derivatization of molecules. UV-VIS Spectroscopy. 1 H-NMR / 13 C-NMR/ IR. TLC, silica-purification, development of mono / multi step reactions, etc. Thermogravimetric assays (TGA) and Differential Thermal Analysis (DTA). (C) Organocatalysis. Proline-like (asymmetric) condensations (Enamine and Imminium-based processes). Organocatalytic-based esterifications (Lactic acid-based). Organocatalytic depolymerization of polysaccharides. Combined concepts bio- and organocatalysis. Organocatalysis and Ionic Liquids. Immobilization of organocatalysts. (D) Bio-Based Society and Biomass Processing. Handling with cellulose, lignocellulose and related compounds. Organocatalytic Biocatalytic concepts (dicarboxylic acids, cellulases). Biofuels 1 st, 2 nd, and 3 rd generation. Bio-based production of chemicals. (E) Microbiological & Biochemical Methodologies. Fermentation (Batch conditions) & Donwstream processing (Centrifugation, concentration of broth culture medium, ultrafiltration and lyophilization). Enzymology. Electrophoresis (SDS-PAGE) and Isoelectric focusing. Purification and quantification of proteins. Handling with wild-type / recombinant enzymes and cells.

9 (F) Management Skills. Supervision of Post-doc, PhD, and Diploma (Master) projects. Supervision of technicians and/or laboratory assistants. Team player. Project Leadership / coordination. Projects on demand Experience in budget acquisition. Preparation of research proposals & reporting to customers. Technical consultancy & advisory. (G) Teaching (RWTH Aachen). (WiSe: Winter Semester; SuSe: Summer Semester). WiSe Praktikum Biocatalysis (Master students). WiSe s 09-10; Modern catalysis. Lectures on Biocatalysis (Master Students). SuSe Praktikum Biocatalysis (Lecture of introduction). SuSe Modern aspects of enzyme technology. (Lectures on organic synthesis for biologists and industrial biocatalysis). Courses Organokatalyse für die Synthese. Organized by Prof. Benjamin List (Max Planck Institute, Germany), and Prof. Albrecht Berkessel (University of Köln). Köln, Germany (sep 2008). Advanced Course on environmental Biotechnology. Kluyver Laboratory for Biotechnology. Delft University of Technology. Delft. The Netherlands (jun 2006). Advanced Course on Biocatalysis. Kluyver Laboratory for Biotechnology. Delft University of Technology. Delft. The Netherlands (apr 2005). Structure based and computed aided drug design. Universidad San Pablo CEU. Madrid. Spain. (oct 2000). Others Regular contributions to workshops & congresses (posters, oral communications, etc). Aug Guest Researcher (1 month) at TNO Quality of Life ( Delft, the Netherlands. Joint project TNO AkzoNobel BV (within B-Basic program), involving fermentation of recombinant microorganisms. As of 2006: Regular reviewer for leading scientific magazines. Feb 2005 Jun 2009: Collaboration as industrial partner (AkzoNobel BV) within the Dutch network Biobased Sustainable Industrial Chemistry (B-BASIC) (

10 July 2009 Onwards. Involved in Excellence Cluster Tailor-made Fuels from Biomass, within RWTH Aachen University. March 2010 Onwards. Project Leader and member of management board of DFG- Graduate College BioNoCo ( Biocatalysis in Non-Conventional media ). Computer Skills: Thorough knowledge of operative systems: Windows applications (Word, Powerpoint, Excel, Chemdraw, etc.). Member of the Spanish Biotechnology Society (SEBIOT) since 1998, and of the European Federation of Biotechnology (EFB) since Hobbies: Travelling, reading, writing, chess, squash, cycling, gardening. Last Update, January 2011

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