HiTrap Heparin HP, 1 ml and 5 ml

Size: px
Start display at page:

Download "HiTrap Heparin HP, 1 ml and 5 ml"

Transcription

1 GE Healthcare Instructions AS HiTrap affinity columns HiTrap Heparin HP, 1 ml and 5 ml HiTrap Heparin HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign.

2 Code No. Designation No. supplied HiTrap Heparin HP 5 1 ml HiTrap Heparin HP 1 5 ml HiTrap Heparin HP 5 5 ml Connectorkit Connectors supplied Usage No. supplied 1/16" male/luer female Connection of syringe to top of HiTrap column 1 Tubing connector Connection of tubing (e.g. Peristaltic flangeless/m6 female Pump P1) to bottom of HiTrap column* 1 Tubing connector Connection of tubing (e.g. Peristaltic flangeless/m6 male Pump P1) to top of HiTrap column** 1 Union 1/16" female/ Connection to original FPLC System M6 male through bottom of HiTrap column 1 Union M6 female/ Connection to original FPLC System 1/16" male through top of HiTrap column 1 Stop plug female, 1/16" Sealing bottom of HiTrap column 2, 5 or 7 * Union 1/16" female/m6 male is also needed. ** Union M6 female/1/16" male is also needed. p.

3 Table of contents 1. Description 3 2. Operation 6 3. Purification 7 4. Scaling up 9 5. Storage 9 6. Further information 9 7. Ordering information 10 p.

4 1. Description Medium properties The nature of Heparin Sepharose High Performance makes it a very versatile tool for the separation of many proteins e.g. DNA binding proteins, growth factors, coagulation protein synthesis factors and steroid receptors. The ligand in Heparin Sepharose High Performance is a naturally occuring sulfated glucosaminoglycan which is extracted from the native proteoglycan of porcine intestinal mucosa. Heparin consists alternating units of uronic acid and D-glucosamine, most of which are substituted with one or two sulfate groups. The molecular weight of the polymer is distributed over the range Heparin is covalently coupled to highly cross-linked agarose beads. The coupling method gives high capacity and high performance. The medium is stable over the ph range 5 10, and tolerates all commonly used aqueous buffers. The properties of the product are summarized in Table 1. Column The columns are made of polypropylene, which is biocompatible and noninteractive with biomolecules. The top and bottom frits are manufactured from porous polyethylene. It is delivered with a stopper on the inlet and a snap-off end on the outlet. The separation can be easily achieved using a syringe with the supplied luer adaptor, a peristaltic pump, or in a chromatography system such as ÄKTAdesign system. Note: To prevent leakage it is essential to ensure that the adaptor is tight. The column cannot be opened or refilled. p. 4

5 Table 1. HiTrap Heparin HP characteristics Column volumes 1 ml and 5 ml Column dimensions i.d. h cm (1 ml) and cm (5 ml) Ligand Heparin Degree of substitution ~10 mg heparin/ml medium Binding capacity ~3 mg antithrombin III (bovine)/ml medium Mean particle size 34 µm Bead structure Highly cross-linked spherical agarose Max. back pressure 0.3 MPa, 3 bar Max. flow rates 4 ml/min and 20 ml/min for 1 ml and 5 ml column respectively Rec. flow rates ml/min and 1 5 ml/min for 1 ml and 5 ml column respectively Chemical stability All commonly used buffers ph stability* Long term 5 10 Short term 5 10 Storage 4 C to 30 C in 20% ethanol * The ranges given are estimates based on our knowledge and experience. Please note the following: ph stability, long term refers to the ph interval where the medium is stable over a long period of time without adverse effects on its subsequent chromatographic performance. ph stability, short term refers to the ph interval for regeneration, cleaning-in-place and sanitization procedures. p.

6 2. Operation The column can be operated with a syringe, peristaltic pump or a chromatography system. Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand; e.g. in its interaction with coagulation factors. Heparin has also a function as a high capacity cation exchanger due to its anionic sulphate groups. Recommended elution conditions for both cases is to increase the ionic strength. Elution using a continuous or step gradient with NaCl, KCl or (NH 4 ) 2 SO 4 up to M is most frequently used. Buffer preparation Water and chemicals used for buffer preparation should be of high purity. It is recommended to filter the buffers by passing them through a 0.45 µm filter before use. Recommended buffers: Binding buffer: 10 mm sodium phosphate, ph ~7 Elution buffer: 10 mm sodium phosphate, 1 2 M NaCl, ph ~7 Sample preparation The sample should be adjusted to the composition of the binding buffer. This can be done by either diluting the sample with binding buffer or by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 column. The sample should be filtered through a 0.45 µm filter or centrifuged immediately before it is applied to the column. p.

7 3. Purification The recommended flow rate for HiTrap Heparin HP is ml/min or 1 5 ml/min for 1 ml or 5 ml column respectively. 1. Fill the syringe or pump tubing with binding buffer. Remove the stopper and connect the column to the syringe (with the provided adaptor), or pump tubing, drop to drop to avoid introducing air into the column. 2. Remove the snap-off end at the column outlet. 3. Wash out the preservative and equilibrate the column with 10 column volumes of binding buffer. 4. Apply the sample, using a syringe fitted to the luer adaptor or by pumping it onto the column. 5. Wash with 5 10 column volumes of binding buffer or until no material appears in the effluent. 6. Elute with 5 10 column volumes of elution buffer using a continuous or step gradient. 7. The purified fractions can be desalted using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 column if necessary (Table 2). Note: If a P-1 pump is used a max flow rate of 1 3 ml/min can be run on a HiTrap 1 ml column packed with Sepharose High Performance media. p. 7

8 Table 3. Prepacked columns for desalting and buffer exchange. Code No Column Loading volume Elution volume Comments Application HiTrap ml ml Prepacked with For desalting and buffer Desalting Sephadex G-25 exchange of protein Superfine. Requires extracts (M r >5000). a syringe or pump to run HiPrep Up to ml Prepacked with For desalting and 26/10 15 ml Sephadex G-25 buffer exchange of Desalting Fine. Requires a protein extracts pump to run. (M r >5000) PD ml 3.5 ml Prepacked with For desalting and Desalting Sephadex G-25. buffer exchange Requires only of protein extracts gravity to run. (M r >5000) NICK 0.1 ml 0.4 ml Prepacked with For separation of Sephadex G-25. proteins (M r >5000) and nicktranslated DNA from radiolabelled nucleotides not shorter than 120 mers, and similar separations NAP ml 1.0 ml Prepacked with For purification of NAP ml 1.5 ml Sephadex G-25 proteins (M r >5000), NAP ml 3.5 ml DNA grade. DNA and oligo- Requires only nucleotides greater gravity to run. than 10 bases in length. p.

9 4. Scaling up For quick scale-up of purification, two or three HiTrap Heparin HP 1 ml or 5 ml columns can be connected in series (backpressure will increase). It is also possible to scale up using the prepacked HiPrep Heparin 16/10 FF column (20 ml). This column is prepacked with Heparin Sepharose Fast Flow. Note: Heparin Sepharose Fast Flow is based on 90 um beads and is another matrix compared to Heparin Sepharose High Performance (34 um beads). It may therefore be necessary with some optimization of the purification method to get exactly the same purity of the target protein. 5. Storage Wash the column with 5 column volumes 20% ethanol at 1 ml/min (HiTrap 1 ml column) or at 5 ml/min (HiTrap 5 ml column). Store the column in 20% ethanol at 4 to 30ºC. 6. Further information For further information, visit Useful handbooks are available, see ordering information. p.

10 7. Ordering information Product No. Supplied Code No. HiTrap Heparin HP 5 1 ml HiTrap Heparin HP 1 5 ml HiTrap Heparin HP 5 5 ml Related products No. Supplied Code No. HiPrep Heparin 16/10 FF 1 (20 ml) HiTrap Desalting 5 5 ml HiTrap Desalting ml* HiPrep 26/10 Desalting 1 (53 ml) HiPrep 26/10 Desalting 4 (53 ml) PD-10 Desalting Column * Special package. Delivered on specific customer order. Accessories No. Supplied Code No. 1/16" male/luer female* Tubing connector flangeless/m6 female* Tubing connector flangeless/m6 male* Union 1/16" female/m6 male* Union M6 female /1/16" male* Union luerlock female/m6 female HiTrap/HiPrep, 1/16" male connector for ÄKTAdesign Stop plug female, 1/16" Fingertight stop plug, 1/16" * One connector included in each HiTrap package. Two, five, or seven stop plugs female included in HiTrap packages depending on the product. One fingertight stop plug is connected to the top of each HiTrap column at delivery. p. 10

11 Related literature No. Supplied Code No. Affinity Chromatography Handbook, Principles and Methods Affinity Chromatography Columns and Media Selection Guide Convenient Protein Purification, HiTrap Column Guide p. 11

12 GE Healthcare Bio-Sciences AB Björkgatan Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire, HP7 9NA UK GE Healthcare Bio-Sciences Corp. 800 Centennial Avenue P.O. Box 1327 Piscataway, NJ USA GE Healthcare Bio-Sciences KK Sanken Bldg Hyakunincho Shinjuku-ku Tokyo Japan GE, imagination at work and GE monogram are trademarks of General Electric Company. ÄKTAdesign, Drop Design, FPLC, HiPrep, HiTrap, NICK, NAP, Sephadex, and Sepharose are trademarks of GE Healthcare companies General Electric Company All rights reserved. First published 1991 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information. imagination at work AS 02/2007 Elanders Östervåla 2007

HiTrap NHS-activated HP

HiTrap NHS-activated HP GE Healthcare Life Sciences Instructions 71-7006-00 AW HiTrap affinity columns HiTrap NHS-activated HP 1 ml and 5 ml HiTrap NHS-activated HP is a prepacked ready to use, column for preparative affinity

More information

HiTrap Desalting, 5 ml

HiTrap Desalting, 5 ml Instructions 71-7154-00 AM HiTrap Desalting, 5 ml HiTrap Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior

More information

HiTrap SP HP, 1 ml and 5 ml HiTrap Q HP, 1 ml and 5 ml

HiTrap SP HP, 1 ml and 5 ml HiTrap Q HP, 1 ml and 5 ml GE Healthcare Instructions 71-7149-00 AN HiTrap ion exchange columns HiTrap SP HP, 1 ml and 5 ml HiTrap Q HP, 1 ml and 5 ml HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange

More information

HiTrap MabSelect SuRe 1 ml and 5 ml

HiTrap MabSelect SuRe 1 ml and 5 ml GE Healthcare Instructions 11-0034-89 AD HiTrap affinity columns HiTrap MabSelect SuRe 1 ml and 5 ml HiTrap MabSelect SuRe is a ready to use column, prepacked with MabSelect SuRe an alkali-tolerant protein

More information

HiTrap Chelating HP, 1 ml and 5 ml

HiTrap Chelating HP, 1 ml and 5 ml GE Healthcare Instructions 71-7005-00 AY HiTrap affinity columns HiTrap Chelating HP, 1 ml and 5 ml HiTrap Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The

More information

HiTrap Desalting HiPrep 26/10 Desalting

HiTrap Desalting HiPrep 26/10 Desalting GE Healthcare Data File 28-9137-87 AA Desalting columns HiTrap Desalting Introduction HiTrap Desalting is a prepacked, ready to use 5 ml column for fast and convenient group separations between high and

More information

Phenyl Sepharose High Performance

Phenyl Sepharose High Performance GE Healthcare Life Sciences Data file 18-1172-87 AD Hydrophobic interaction chromatography Phenyl Sepharose High Performance Butyl Sepharose High Performance Phenyl Sepharose High Performance and Butyl

More information

HiTrap SP HP, 1 ml and 5 ml HiTrap Q HP, 1 ml and 5 ml

HiTrap SP HP, 1 ml and 5 ml HiTrap Q HP, 1 ml and 5 ml Instructions 71-7149-00 AP HiTrap SP HP, 1 ml and 5 ml HiTrap Q HP, 1 ml and 5 ml HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations,

More information

Benzamidine Sepharose 4 Fast Flow (high sub) HiTrap Benzamidine FF (high sub)

Benzamidine Sepharose 4 Fast Flow (high sub) HiTrap Benzamidine FF (high sub) GE Healthcare Data File 18-1139-38 AC Affinity chromatography Benzamidine Sepharose 4 Fast Flow (high sub) HiTrap Benzamidine FF (high sub) HiTrap Benzamidine FF (high sub) are prepacked, ready to use

More information

Q Sepharose High Performance

Q Sepharose High Performance GE Healthcare Life Sciences Data file 18-1172-88 AC Ion Exhange Chromatography Q Sepharose High Performance SP Sepharose High Performance Q Sepharose High Performance and SP Sepharose High Performance

More information

Glutathione Sepharose High Performance GSTrap HP

Glutathione Sepharose High Performance GSTrap HP GE Healthcare Data File 8-74-32 AC Affinity chromatography Glutathione Sepharose High Performance GSTrap HP Glutathione Sepharose High Performance is a high performance affinity medium for the purification

More information

Code No. Designation Type of Gel No. supplied Connectors Description Column

Code No. Designation Type of Gel No. supplied Connectors Description Column instructions HiTrap HiTrap DEAE FF, 1 ml and 5 ml HiTrap CM FF, 1 ml and 5 ml HiTrap Q FF, 1 ml and 5 ml HiTrap SP FF, 1 ml and 5 ml HiTrap ANX FF (high sub), 1 ml and 5 ml HiTrap ion exchange columns

More information

HiTrap MabSelect SuRe,

HiTrap MabSelect SuRe, Instructions 11-0034-89 AF HiTrap MabSelect SuRe, 1 ml and 5 ml HiTrap MabSelect SuRe is a ready-to-use column, prepacked with MabSelect SuRe, an alkali-tolerant protein A-derived medium for capturing

More information

HiTrap affinity columns

HiTrap affinity columns instructions HiTrap affinity columns HiTrap Chelating HP, 1 ml and 5 ml HiTrap TM Chelating HP is a pre-packed ready to use, disposable column for preparative affinity chromatography. The special design

More information

His Buffer Kit is intended for research use only, and should not be used in any clinical or in vitro procedures for diagnostic purposes.

His Buffer Kit is intended for research use only, and should not be used in any clinical or in vitro procedures for diagnostic purposes. GE Healthcare Instructions 28-4010-39 AC His Buffer Kit Intended use His Buffer Kit is intended for research use only, and should not be used in any clinical or in vitro procedures for diagnostic purposes.

More information

Code No. Designation No. supplied

Code No. Designation No. supplied instructions HiTrap HiTrap Phenyl FF (high sub), 1 ml and 5 ml HiTrap Phenyl FF (low sub), 1 ml and 5 ml HiTrap Phenyl HP, 1 ml and 5 ml HiTrap Butyl FF, 1 ml and 5 ml HiTrap Octyl FF, 1 ml and 5 ml HiTrap

More information

Phenyl Sepharose CL-4B

Phenyl Sepharose CL-4B GE Healthcare Instructions 71-7080-00 AE Hydrophobic interaction chromatography Phenyl Sepharose CL-4B Phenyl Sepharose TM CL-4B is a separation medium for hydrophobic interaction chromatography (HIC).

More information

HiTrap IEX Selection Kit

HiTrap IEX Selection Kit Instructions 71-5017-52 AK HiTrap IEX Selection Kit HiTrap SP FF, 1 ml HiTrap CM FF, 1 ml HiTrap Q FF, 1 ml HiTrap DEAE FF, 1 ml HiTrap ANX FF (high sub), 1 ml HiTrap Q XL, 1 ml HiTrap SP XL, 1 ml HiTrap

More information

Instructions AH

Instructions AH Instructions 71-5017-51 AH HiTrap DEAE FF, 1 ml and 5 ml HiTrap CM FF, 1 ml and 5 ml HiTrap Q FF, 1 ml and 5 ml HiTrap SP FF, 1 ml and 5 ml HiTrap ANX FF (high sub), 1 ml and 5 ml HiTrap SP FF, HiTrap

More information

GE Healthcare. GammaBind G Sepharose

GE Healthcare. GammaBind G Sepharose GE Healthcare Instructions 71-7057-00 AH Affinity Media GammaBind G Sepharose GammaBind G Sepharose is GammaBind G, Type 2, covalently immobilized to Sepharose 4B by the cyanogen bromide method. GammaBind

More information

HiTrap MabSelect, 1 ml and 5 ml HiTrap MabSelect Xtra, 1 ml and 5 ml

HiTrap MabSelect, 1 ml and 5 ml HiTrap MabSelect Xtra, 1 ml and 5 ml Instructions 28-4084-14 AD HiTrap MabSelect, 1 ml and 5 ml HiTrap MabSelect Xtra, 1 ml and 5 ml HiTrap MabSelect and HiTrap MabSelect Xtra are 1 ml and 5 ml ready to use columns, prepacked with MabSelect

More information

Convenient Purification of Monoclonal Antibodies using HiTrap rprotein A FF

Convenient Purification of Monoclonal Antibodies using HiTrap rprotein A FF GE Healthcare Convenient Purification of Monoclonal Antibodies using HiTrap rprotein A FF M. Carlsson, A. Heijbel, and A-C. Häggqvist GE Healthcare AB, Björkgatan 3, SE-751 84 Uppsala, Sweden Abstract

More information

HiTrap rprotein A FF HiTrap Protein A HP HiTrap Protein G HP

HiTrap rprotein A FF HiTrap Protein A HP HiTrap Protein G HP Data file 11-0035-58 AB Affinity chromatography HiTrap rprotein A FF HiTrap Protein A HP HiTrap Protein G HP HiTrap rprotein A FF, HiTrap Protein A HP and HiTrap Protein G HP are part of the range of prepacked,

More information

HiTrap Capto adhere, 1 ml and 5 ml

HiTrap Capto adhere, 1 ml and 5 ml Instructions 28-9063-51 AC HiTrap Capto adhere, 1 ml and 5 ml Capto adhere is a multimodal BioProcess medium for intermediate purification and polishing of monoclonal antibodies after capture on Protein

More information

2 5 ADP Sepharose 4B. GE Healthcare Life Sciences

2 5 ADP Sepharose 4B. GE Healthcare Life Sciences GE Healthcare Life Sciences Instructions 71-7092-00 AF Affinity chromatography 2 5 ADP Sepharose 4B 2 5 ADP Sepharose 4B interacts strongly with NADP + -dependent dehydrogenases. Selective elution with

More information

Ni Sepharose excel, 100 ml and 500 ml HisTrap excel, 1 ml and 5 ml

Ni Sepharose excel, 100 ml and 500 ml HisTrap excel, 1 ml and 5 ml GE Healthcare Life Sciences Chromatography medium 29-0138-67 AC Prepacked columns Ni Sepharose excel, 100 ml and 500 ml HisTrap excel, 1 ml and 5 ml Ni Sepharose excel is an immobilized metal ion affinity

More information

SOURCE 15HIC. GE Healthcare. SOURCE 15ETH, SOURCE 15ISO, SOURCE 15PHE, SOURCE 15PHE 4.6/100 PE (Tricorn ), Characteristics

SOURCE 15HIC. GE Healthcare. SOURCE 15ETH, SOURCE 15ISO, SOURCE 15PHE, SOURCE 15PHE 4.6/100 PE (Tricorn ), Characteristics GE Healthcare Data file 18-1128-86 AC High-performance hydrophobic interaction chromatography SOURCE 15HIC SOURCE 15ETH, SOURCE 15ISO, SOURCE 15PHE, SOURCE 15PHE 4.6/100 PE (Tricorn ), Resource ETH, Resource

More information

Affinity purification using ÄKTA start

Affinity purification using ÄKTA start GE Healthcare Life Sciences Affinity purification using ÄKTA start Training cue card This protocol will help you understand the practical principles of affinity chromatography by taking you step-by-step

More information

HiTrap HIC columns. Instructions AG

HiTrap HIC columns. Instructions AG Instructions 11-0034-52 AG HiTrap HIC columns HiTrap Phenyl FF (high sub), 1 ml and 5 ml HiTrap Phenyl FF (low sub), 1 ml and 5 ml HiTrap Phenyl HP, 1 ml and 5 ml HiTrap Butyl FF, 1 ml and 5 ml HiTrap

More information

Gel filtration using ÄKTA start

Gel filtration using ÄKTA start GE Healthcare Life Sciences Gel filtration using ÄKTA start Training cue card This protocol will help you understand the practical principles of gel filtration by taking you step-by-step through the purification

More information

Albumin & IgG Depletion SpinTrap

Albumin & IgG Depletion SpinTrap GE Healthcare Albumin & IgG Depletion SpinTrap Albumin & IgG Depletion SpinTrap is designed for depletion of albumin and IgG from human plasma or serum. Product booklet Code: 28-9480-20 Page finder 1.

More information

Protein A Mag Sepharose Xtra Protein G Mag Sepharose Xtra

Protein A Mag Sepharose Xtra Protein G Mag Sepharose Xtra GE Healthcare Instructions 28-9670-57 AA Mag Sepharose Protein A Mag Sepharose Xtra Protein G Mag Sepharose Xtra Protein A Mag Sepharose Xtra and Protein G Mag Sepharose Xtra are available in the following

More information

Instructions AD Hydrophobic interaction

Instructions AD Hydrophobic interaction GE Healthcare Instructions 56-1191-01 AD Hydrophobic interaction Phenyl Sepharose 6 Fast Flow (low sub) Phenyl Sepharose 6 Fast Flow (high sub) Butyl-S Sepharose 6 Fast Flow Butyl Sepharose 4 Fast Flow

More information

Glutathione Sepharose 4 Fast Flow GSTPrep FF 16/10 GSTrap FF

Glutathione Sepharose 4 Fast Flow GSTPrep FF 16/10 GSTrap FF GE Healthcare Data file 8-74-85 AC Affinity chromatography Glutathione Sepharose 4 Fast Flow GSTPrep FF 6/ GSTrap FF Glutathione Sepharose 4 Fast Flow is an affinity chromatography medium for the isolation

More information

Gelatin Sepharose. instructions

Gelatin Sepharose. instructions instructions Gelatin Sepharose Gelatin Sepharose 4B is gelatin coupled to Sepharose 4B by the cyanogen bromide method. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein

More information

Protein A Mag Sepharose Xtra Protein G Mag Sepharose Xtra

Protein A Mag Sepharose Xtra Protein G Mag Sepharose Xtra GE Healthcare Data file 28-9768-1 AA Protein sample preparation Protein A Mag Sepharose Xtra Xtra products are magnetic beads designed for efficient, high capacity small-scale purification/screening of

More information

Columns C 10, C 16, C 26

Columns C 10, C 16, C 26 GE Healthcare Instructions 59-5261-00 AL Columns C 10, C 16, C 26 The C-series columns are designed for Standard Chromatography. The eluent inlet pieces incorporate a reverse funnel design that distributes

More information

Ab SpinTrap Ab Buffer Kit

Ab SpinTrap Ab Buffer Kit GE Healthcare Data File 28-9020-30 AB Protein Sample Preparation Ab SpinTrap Ab Buffer Kit The Ab SpinTrap and the Protein G HP SpinTrap are identical columns. The difference between the article numbers

More information

HiScreen MabSelect SuRe HiScreen MabSelect SuRe LX HiScreen MabSelect HiScreen MabSelect Xtra

HiScreen MabSelect SuRe HiScreen MabSelect SuRe LX HiScreen MabSelect HiScreen MabSelect Xtra GE Healthcare Life Sciences Instructions 28-9339-49 AD HiScreen affinity columns HiScreen MabSelect SuRe HiScreen MabSelect SuRe LX HiScreen MabSelect HiScreen MabSelect Xtra Introduction HiScreen MabSelect

More information

rprotein A GraviTrap Protein G GraviTrap rprotein A/Protein G GraviTrap

rprotein A GraviTrap Protein G GraviTrap rprotein A/Protein G GraviTrap GE Healthcare Life Sciences Data file 28-9921-04 AA rprotein A GraviTrap Protein G GraviTrap rprotein A/Protein G GraviTrap Protein sample preparation rprotein A GraviTrap, Protein G GraviTrap, and rprotein

More information

His Mag Sepharose excel

His Mag Sepharose excel GE Healthcare Life Sciences Instructions 29-0163-14 AC Protein sample preparation His Mag Sepharose excel His Mag Sepharose excel is a magnetic immobilized metal ion affinity chromatography (IMAC) medium

More information

Ni Sepharose 6 Fast Flow HisPrep FF 16/10 HisTrap FF

Ni Sepharose 6 Fast Flow HisPrep FF 16/10 HisTrap FF Data file 11-8-86 AF Affinity chromatography Ni Sepharose 6 Fast Flow HisPrep FF 16/1 HisTrap FF Purifying histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC) continues

More information

Table of contents. p. 2

Table of contents. p. 2 GE Healthcare Instructions 56-1190-98 AE Gel Filtration Media Sephacryl S-100 High Resolution Sephacryl S-200 High Resolution Sephacryl S-300 High Resolution Sephacryl S-400 High Resolution Sephacryl S-500

More information

Blue Sepharose CL-6B. instructions

Blue Sepharose CL-6B. instructions instructions Blue Sepharose CL-6B Blue Sepharose CL-6B is Cibacron Blue F3G-A covalently attached to Sepharose CL-6B by the triazine coupling method. The structure of the blue dye makes it a very versatile

More information

Arginine Sepharose 4B

Arginine Sepharose 4B instructions Arginine Sepharose 4B Arginine Sepharose 4B is L-arginine immobilized to Sepharose 4B by an epoxy coupling method via a long hydrophilic spacer and stable ether and alkylamine bonds. L-arginine

More information

Capto * Blue and Capto Blue (high sub)

Capto * Blue and Capto Blue (high sub) GE Healthcare Life Sciences Data file 28-9369-6 AC Affinity chromatography Capto * Blue and (high sub) and (high sub) are affinity chromatography media (resins) for the capture of human serum albumin,

More information

HisTrap FF crude, 1 ml and 5 ml

HisTrap FF crude, 1 ml and 5 ml GE Healthcare Instructions 11-0012-38 AF HisTrap affinity columns HisTrap FF crude, 1 ml and 5 ml HisTrap FF crude is a ready to use column, prepacked with precharged Ni Sepharose 6 Fast Flow. This prepacked

More information

Protein A HP SpinTrap

Protein A HP SpinTrap GE Healthcare Instructions 28-9067-70 AB Protein A HP SpinTrap Protein A HP SpinTrap contains 16 prepacked Protein A HP SpinTrap columns 1 plastic bottom cap removal tool Instructions for use Introduction

More information

Glutathione Sepharose and prepacked columns

Glutathione Sepharose and prepacked columns Data file 28-9941-47 AB Affinity chromatography Glutathione Sepharose and prepacked columns Glutathione Sepharose 4B, Glutathione Sepharose 4 Fast Flow (FF), and Glutathione Sepharose High Performance

More information

Glutathione Sepharose and prepacked columns

Glutathione Sepharose and prepacked columns GE Healthcare Life Sciences Data file 28-9941-47 AA Affinity chromatography Glutathione Sepharose and prepacked columns Glutathione Sepharose 4B, Glutathione Sepharose 4 Fast Flow (FF), and Glutathione

More information

Capto Blue and Capto Blue (high sub)

Capto Blue and Capto Blue (high sub) Data file 28-9369-6 AD Affinity chromatography Capto Blue and (high sub) and (high sub) are affinity chromatography media (resins) for the capture of human serum albumin (HSA), as well as purification

More information

Streptavidin Mag Sepharose

Streptavidin Mag Sepharose GE Healthcare Life Sciences Data file 28-9921-05 AB Protein sample preparation Streptavidin Mag Sepharose Streptavidin Mag Sepharose (Fig 1) is a magnetic bead for simple and efficient enrichment of target

More information

data file Affinity chromatography

data file Affinity chromatography data file Affinity chromatography HiTrap Chelating HP is one of a range of prepacked, ready to use, 1 ml and 5 ml columns for preparative metal chelate affinity chromatography. Fast, simple, and easy separations

More information

Sample preparation for analysis of proteins, peptides and carbohydrates

Sample preparation for analysis of proteins, peptides and carbohydrates GE Healthcare Life Sciences Sample preparation for analysis of proteins, peptides and carbohydrates Desalting, Buffer Exchange, Cleanup, Concentration Selection guide The Trap platform The Trap platform

More information

5 AMP Sepharose 4B. instructions

5 AMP Sepharose 4B. instructions instructions 5 AMP Sepharose 4B 5' AMP Sepharose 4B interacts strongly with NAD + -dependent dehydrogenases and ATP-dependent enzymes. Selective elution with gradients of NAD + or NADP + has allowed the

More information

G Trap Ion Exchange Selection Kit

G Trap Ion Exchange Selection Kit G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name G Trap Ion Exchange Selection Kit CM, DEAE, Q and SP Agarose Fast Flow columns (Cat. # 786

More information

HisTrap HP Kit. instructions

HisTrap HP Kit. instructions instructions HisTrap HP Kit Caution! Contains nickel. May produce an allergic reaction. for purification of His-tagged proteins HisTrap HP Kit is a kit for convenient, fast, and efficient purification

More information

SOURCE 15Q, SOURCE 15S, and prepacked column formats

SOURCE 15Q, SOURCE 15S, and prepacked column formats GE Healthcare Life Sciences Data file 18-1123-65 AF Ion exchange chromatography SOURCE 15Q, SOURCE 15S, and prepacked column formats SOURCE 15Q and SOURCE 15S are high performance ion exchange chromatography

More information

AVB Sepharose High Performance

AVB Sepharose High Performance GE Healthcare Life Sciences Data file 28-927-54 AB Custom designed media AVB Sepharose High Performance AVB Sepharose High Performance is an affinity chromatography medium (resin) designed for the purification

More information

His GraviTrap. GE Healthcare. Operation

His GraviTrap. GE Healthcare. Operation GE Healthcare Data File 11-0036-90 AA GraviTrap column His GraviTrap His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography

More information

Two-step purification with ÄKTA pure, or ÄKTA avant, using loop collection Cue Card

Two-step purification with ÄKTA pure, or ÄKTA avant, using loop collection Cue Card Two-step purification with ÄKTA pure, or ÄKTA avant, using loop collection Cue Card Contents Principles 2 Setup for multi-step purification 2 UNICORN methods 3 How to add user defined phases to a method

More information

MabSelect SuRe. GE Healthcare. Alkali-stabilized protein A-derived medium for capture of monoclonal antibodies. High stability in alkaline conditions

MabSelect SuRe. GE Healthcare. Alkali-stabilized protein A-derived medium for capture of monoclonal antibodies. High stability in alkaline conditions GE Healthcare Data File 11-11-65 AB Affinity chromatography MabSelect SuRe Alkali-stabilized protein A-derived medium for capture of monoclonal antibodies MabSelect SuRe (Superior Resistance) is a member

More information

Phenyl Sepharose High Performance Butyl Sepharose High Performance

Phenyl Sepharose High Performance Butyl Sepharose High Performance Instructions 71-7129-00 AF Hydrophobic interaction Phenyl Sepharose High Performance Butyl Sepharose High Performance Phenyl Sepharose TM High Performance and Butyl Sepharose High Performance are members

More information

CNBr-activated Sepharose 4 Fast Flow

CNBr-activated Sepharose 4 Fast Flow GE Healthcare Instructions 71-5000-15 AD Pre-activated media CNBr-activated Sepharose 4 Fast Flow Introduction The preparation and use of affinity chromatography media by coupling biospecific ligands to

More information

Protein A HP MultiTrap

Protein A HP MultiTrap GE Healthcare Instructions 28-9067-71 AC Protein A HP MultiTrap Protein A HP MultiTrap contains 4 prepacked Protein A HP MultiTrap 96-well filter plates Instructions for use Introduction Protein A HP MultiTrap

More information

Streptavidin HP SpinTrap Streptavidin HP MultiTrap Streptavidin HP SpinTrap Buffer Kit

Streptavidin HP SpinTrap Streptavidin HP MultiTrap Streptavidin HP SpinTrap Buffer Kit GE Healthcare Data File 28-9067-91 AB Protein Sample Preparation Streptavidin HP SpinTrap Streptavidin HP MultiTrap Streptavidin HP SpinTrap Buffer Kit Streptavidin HP SpinTrap and Streptavidin HP MultiTrap

More information

IEX Sepharose Fast Flow labpacks and prepacked columns

IEX Sepharose Fast Flow labpacks and prepacked columns GE Healthcare Data file -77- Ion exchange chromatography IEX Sepharose Fast Flow labpacks and prepacked columns The popularity of Sepharose Fast Flow ion exchangers reflects the leading role they play

More information

PlasmidSelect Xtra PlasmidSelect Xtra Screening Kit PlasmidSelect Xtra Starter Kit

PlasmidSelect Xtra PlasmidSelect Xtra Screening Kit PlasmidSelect Xtra Starter Kit Data file 8-494-87 AB Plasmid purification media PlasmidSelect Xtra PlasmidSelect Xtra Screening Kit PlasmidSelect Xtra Starter Kit Purified plasmid DNA is required in increasingly larger quantities to

More information

CNBr-activated Sepharose 4B

CNBr-activated Sepharose 4B GE Healthcare Instructions 71-7086-00 AF Affinity media CNBr-activated Sepharose 4B CNBr-activated Sepharose 4B is a pre-activated medium for immobilization of ligands containing primary amines. It provides

More information

Regeneration Scouting Kit

Regeneration Scouting Kit GE Healthcare Life Sciences Instruction 22-0618-29 AC Biacore Regeneration Scouting Kit Product description Order code: Contents: Storage: Safety: BR-1005-56 Ethylene glycol (p.a.), 11 ml Glycine-HCl,

More information

Protein G HP SpinTrap / Ab Spin Trap

Protein G HP SpinTrap / Ab Spin Trap GE Healthcare Life Sciences Protein G HP SpinTrap / Ab Spin Trap Product booklet Codes: 28-9031-34 28-4083-47 Page finder 1. Legal 3 2. Handling 4 2.1. Safety warnings and precautions 4 2.2. Storage 4

More information

Sephacryl S-100, S-200, S-300, S-400, S-500 High Resolution

Sephacryl S-100, S-200, S-300, S-400, S-500 High Resolution GE Healthcare Sephacryl S-100, S-200, S-300, S-400, S-500 High Resolution Instructions Sephacryl 2 Instructions 52-2086-00 AK Contents 1. Introduction 5 2. To prepare the medium suspension 6 3. To pack

More information

Heparin Sepharose 6 Fast Flow

Heparin Sepharose 6 Fast Flow GE Healthcare Instructions 71-7190-00 AE Affinity media Heparin Sepharose 6 Fast Flow Heparin is a naturally occurring glycosaminoglycan which serves as an effective affinity binding and ion exchange ligand

More information

HiTrap IMAC HP, 1 ml and 5 ml

HiTrap IMAC HP, 1 ml and 5 ml Instructions 28-4046-23 AE HiTrap IMAC HP, 1 ml and 5 ml HiTrap IMAC HP is prepacked with IMAC Sepharose High Performance. The column is ideal for purification of histidine-tagged proteins and other proteins/peptides

More information

Protein A HP SpinTrap

Protein A HP SpinTrap GE Healthcare Protein A HP SpinTrap Product booklet Code: 28-9031-32 Page finder 1. Legal 3 2. Handling 4 2.1. Safety warnings and precautions 4 2.2. Storage 4 2.3 Expiry 4 3. Introduction 5 4. General

More information

illustra NAP-5 Columns Gravity flow columns for the purification of oligonucleotides and small DNA fragments, desalting and buffer exchange

illustra NAP-5 Columns Gravity flow columns for the purification of oligonucleotides and small DNA fragments, desalting and buffer exchange GE Healthcare illustra NAP-5 Columns Gravity flow columns for the purification of oligonucleotides and small DNA fragments, desalting and buffer exchange Product booklet See back cover for quick reference

More information

BabyBio S BabyBio Q BabyBio DEAE

BabyBio S BabyBio Q BabyBio DEAE DATA SHEET BabyBio S BabyBio Q BabyBio DEAE BabyBio S, BabyBio Q and BabyBio DEAE are pre-packed, ready to use ion exchange columns for easy and convenient purification of proteins. Rapid method screening

More information

Ni Sepharose 6 Fast Flow HisPrep FF 16/10 HisTrap FF

Ni Sepharose 6 Fast Flow HisPrep FF 16/10 HisTrap FF GE Healthcare Data file 11-8-86 AE Ni Sepharose 6 Fast Flow HisPrep FF 16/1 HisTrap FF Affinity chromatography Purifying histidine-tagged recombinant proteins by immobilized metal affinity chromatography

More information

Capture of human single-chain Fv (scfv) fusion protein on Capto L affinity medium

Capture of human single-chain Fv (scfv) fusion protein on Capto L affinity medium GE Healthcare Life Sciences Application note 29-0144-56 AA Affinity chromatography Capture of human single-chain Fv (scfv) fusion protein on Capto L affinity medium We describe the capture of a single-chain

More information

SOURCE 15Q and SOURCE 15S

SOURCE 15Q and SOURCE 15S GE Healthcare Instructions 18-1060-43 AF Ion exchange media SOURCE 15Q and SOURCE 15S SOURCE 15Q and SOURCE 15S are strong anion and cation exchange media based on polystyrene/divinyl benzene monodisperse

More information

GSTrap FF, 1 ml and 5 ml

GSTrap FF, 1 ml and 5 ml Instructions 71-5016-96 AM GSTrap FF, 1 ml and 5 ml GSTrap FF columns are prepacked 1 ml and 5 ml HiTrap columns for convenient, one-step purification of glutathione S-transferase (GST) tagged proteins

More information

Superdex 200 Increase columns

Superdex 200 Increase columns GE Healthcare Life Sciences Data file 29-452-69 AA Size exclusion chromatography Superdex 2 Increase columns Superdex 2 Increase prepacked columns (Fig 1) are designed for size exclusion chromatography

More information

Connecting tubing (e.g. Peristaltic 1 Pump P1) to bottom of HiTrap column 1

Connecting tubing (e.g. Peristaltic 1 Pump P1) to bottom of HiTrap column 1 GE Healthcare Life Sciences Instructions 28-9574-96 AC Affinity chromatography HiTrap TALON crude, 1 ml and 5 ml TALON Superflow TALON Superflow chromatography medium is recommended for batch/ gravity-flow

More information

Instruction AB

Instruction AB GE Healthcare Life Sciences Instruction 28-9242-34 AB Biacore Biotin CAPture Kit Product description Order code: Contents: Storage: Kit capacity: 28-9202-33 (Biotin CAPture Kit) 28-9202-34 (Biotin CAPture

More information

Polishing of monoclonal antibodies using Capto S ImpAct

Polishing of monoclonal antibodies using Capto S ImpAct Application note 29-83-27 AA Ion exchange chromatography Polishing of monoclonal antibodies using Capto S ImpAct Capto S ImpAct chromatography medium (resin) is a strong cation exchanger (CIEX). The medium

More information

Superdex 200 Increase columns

Superdex 200 Increase columns Data file 29-42-69 AC Size exclusion chromatography Superdex 2 Increase columns Superdex 2 Increase prepacked columns (Fig 1) are designed for size exclusion chromatography (SEC)/high resolution gel filtration

More information

Superdex prep grade. GE Healthcare Life Sciences

Superdex prep grade. GE Healthcare Life Sciences GE Healthcare Life Sciences Instructions 18-1060-29 AE Gel filtration chromatography Superdex prep grade Superdex prep grade (pg) is a preparative gel filtration chromatography medium; part of BioProcess

More information

MabSelect SuRe pcc. Medium characteristics. GE Healthcare. Affinity chromatography. Optimized for continuous chromatography applications

MabSelect SuRe pcc. Medium characteristics. GE Healthcare. Affinity chromatography. Optimized for continuous chromatography applications GE Healthcare Affinity chromatography MabSelect SuRe pcc MabSelect SuRe pcc affinity chromatography medium (resin) offers exceptional capacity at short residence time, making it well-suited for applications

More information

Capto Q Capto ViralQ Capto S

Capto Q Capto ViralQ Capto S GE Healthcare Data File 11-25-76 AD Ion exchange chromatography Capto ViralQ Capto Q and are strong ion exchange media for packed bed chromatography that increase speed and throughput in capture and intermediate

More information

HiLoad Superdex 30 prep grade HiLoad Superdex 75 prep grade HiLoad Superdex 200 prep grade

HiLoad Superdex 30 prep grade HiLoad Superdex 75 prep grade HiLoad Superdex 200 prep grade GE Healthcare Life Sciences Data file 8--52 AE Gel filtration HiLoad Superdex 3 prep grade HiLoad Superdex 75 prep grade HiLoad Superdex 2 prep grade HiLoad columns are XK laboratory columns prepacked

More information

Streptavidin HP SpinTrap Streptavidin HP MultiTrap

Streptavidin HP SpinTrap Streptavidin HP MultiTrap GE Healthcare Data File 28-9067-91 AA Protein enrichment Streptavidin HP SpinTrap Streptavidin HP MultiTrap Streptavidin HP SpinTrap and Streptavidin HP MultiTrap toolkits (Fig 1) are prepacked, single-use

More information

PlasmidSelect Xtra PlasmidSelect Xtra Screening Kit PlasmidSelect Xtra Starter Kit

PlasmidSelect Xtra PlasmidSelect Xtra Screening Kit PlasmidSelect Xtra Starter Kit GE Healthcare Data File 28-494-87 AA Plasmid purification media PlasmidSelect Xtra PlasmidSelect Xtra Screening Kit PlasmidSelect Xtra Starter Kit Purified plasmid DNA is required in increasingly larger

More information

Predictable scale-up through column design and robust packing methodology

Predictable scale-up through column design and robust packing methodology GE Healthcare Predictable scale-up through column design and robust packing methodology Johan Karlsson, Johan Tschöp, and Joakim Svensson. Posters presented at IBC BioProcess International Conference and

More information

Capto Q. GE Healthcare. Media characteristics. High flow rates and low backpressure in large-scale columns

Capto Q. GE Healthcare. Media characteristics. High flow rates and low backpressure in large-scale columns GE Healthcare Data File 11-25-76 AB Ion exchange chromatography Capto Q is a strong anion exchange medium for packed bed chromatography that allows increased speed and throughput in capture and intermediate

More information

High-throughput screening and optimization of a multimodal polishing step in a monoclonal antibody purification process

High-throughput screening and optimization of a multimodal polishing step in a monoclonal antibody purification process GE Healthcare Application note 28-99- AB High-throughput process development High-throughput screening and optimization of a multimodal polishing step in a monoclonal antibody purification process Key

More information

Bio-Scale Mini Affi-Prep Protein A Cartridges, 1 and 5 ml. Instruction Manual. Catalog # ,

Bio-Scale Mini Affi-Prep Protein A Cartridges, 1 and 5 ml. Instruction Manual. Catalog # , Bio-Scale Mini Affi-Prep Protein A Cartridges, 1 and 5 ml Instruction Manual Catalog # 732-4600, 732-4602 Table of Contents Section 1...Introduction...1 Section 2 Section 3 Section 4 Connecting to Bio-Rad

More information

MabSelect. GE Healthcare. Characteristics

MabSelect. GE Healthcare. Characteristics GE Healthcare Data File 18-1149-94 AE Affinity chromatography is a BioProcess affinity medium for capturing monoclonal antibodies from large volumes of feed by packed bed chromatography. offers the following

More information

TALON Superflow and prepacked formats

TALON Superflow and prepacked formats Data file 28-9664-1 AC Affinity chromatography TALON Superflow and prepacked formats TALON Superflow is a cobalt-based immobilized metal affinity chromatography medium (IMAC) offering enhanced selectivity

More information

Protein G HP SpinTrap Protein G HP MultiTrap Protein A/G SpinTrap Buffer Kit

Protein G HP SpinTrap Protein G HP MultiTrap Protein A/G SpinTrap Buffer Kit GE Healthcare Data File 28-9067-90 AB Protein Sample Preparation Protein G HP SpinTrap Protein A/G SpinTrap Buffer Kit Protein G HP SpinTrap and (Fig 1) are designed for the enrichment of proteins of interest,

More information

MabSelect SuRe. MabSelect. GE Healthcare

MabSelect SuRe. MabSelect. GE Healthcare GE Healthcare Instructions 11-0026-01 AC Affinity Chromatography MabSelect SuRe MabSelect SuRe is a novel, alkali-tolerant protein A-derived medium for capturing monoclonal antibodies from large volumes

More information