Conversion of Sucrose into Palatinose with Immobilized Serratia Plymuthica Cells
|
|
- Noah Lane
- 6 years ago
- Views:
Transcription
1 7 Bulgarian Journal of Agricultural Science, (6), 7-76 National Centre for Agrarian Sciences Conversion of Sucrose into Palatinose with Immobilized Serratia Plymuthica Cells A. KRASTANOV, D. BLAZHEVA and Toshiomi YOSHIDA University of Food Technology, Department of Biotechnology, BG - 4 Plovdiv, Bulgaria ICBiotech, Osaka University, Osaka, Japan Abstract KRASTANOV, A., D. BLAZHEVA and Toshiomi YOSHIDA, 6. Conversion of sucrose into palatinose with immobilized Serratia plymuthica cells. Bulg. J. Agric. Sci., : 7-76 Palatinose (isomaltulose) can be used as a substitute of sucrose. Here we describe a simple and effective method of converting concentrated sucrose solutions into palatinose using immobilized Serratia plymuthica cells. The cells were immobilized in alginate beads and on chitin. A conversion rate of up to 99 % was achieved. The process was carried out in a batch and continuous mode in a column. The effect of temperature and flow rate on the conversion rate and the productivity of the column was investigated. Key words: Palatinose, Serratia plymuthica, immobilized cells, alginate, chitin, conversion rate, productivity Introduction Palatinose (isomaltulose, 6-O-αglucopiranosyl-D-fructose) is a structural isomer of sucrose with similar physicochemical, chemical and organoleptic properties. It is not metabolized by the bacteria causing caries and after its consumption the levels of the blood monosaccharides remain low (Goda et al., 983 and Minami et al., 99). Due to this facts palatinose is successfully applied as a sucrose alternative and the interest towards its production on an industrial scale is growing. Lately, the technologies employing immobilized cells for conversion of sucrose into paltinose have almost completely replaced the conventional chemical and enzyme technologies, and the annual production of palatinose exceeds 5 t. At present, as potential microorganisms, which can be used in an immobilized form for the conversion of sucrose into palatinose, are thoroughly studied Protaminobacter rubrum (Mizutani, 99) and Erwinia rhapontici (Cheetham et al., 985). Taking into consideration the investigations of a number of scientists
2 7 (McAllister et al., 99 and Veronese et al., 999) on the biosynthesis and the properties of α-glucosyltransferase (sucrose isomerase) from Serratia plymuthica, a matter of interest was the application of immobilized cells of this microorganism for bioconversion of sucrose into palatinose. Materials and Methods A. Krastanov, D. Blazheva and T. Yoshida The standard inoculum of Serratia plymuthica ATCC 598 cells was prepared in 5 cm 3 nutrient medium with the following composition (%): sucrose - 5., beef extract -.3, bacto peptone -., yeast extract -.5, NaCl -.3, Na HPO 4 -. until the culture reached OD 6 =.4-.. The inoculum (%) was then transferred into cm 3 nutrient medium with the same composition (ph 6.8) and the cells were cultivated at 8 C. The cell suspension was centrifuged at 8 rpm for min. The biomass was analyzed for enzyme activity and immobilized in alginate and on the surface of chitin. The immobilization in alginate (3%) was realized through adding of the alginate dropwise in a CaCl solution. The immobilization on the surface of chitin was carried out through mixing of the cell suspension (.5 g/cm 3 ) with chitin and glutraldehyde, which was followed by several hours of stirring at room temperature and ph 6.. The conversion of sucrose into palatinose with the immobilized in alginate cells was accomplished in a batch enzyme reactor, and with those immobilized on chitin - in a tubular reactor (H=3 mm, D=5mm, V=5cm 3 ) with equipment for temperature and flow rate control. The enzyme activity was determined through the incubation of cm 3 enzyme solution (or a definite quantity of immobilized preparation) with cm 3 (5 cm 3 for the immobilized preparations) 4% sucrose solution in 5 mm phosphate buffer with ph 6. at temperature 3 C. The palatinose content was measured by the Somogy-Nelson method as a reducing sugar. The activity of the free cells was analyzed after their ultrasonic disintegration and the determination of the sucrose isomerase activity of the liquid phase. The conversion of sucrose into palatinose was studied through thin layer chromatography by the Adachi method (Adachi, 965). Results and Discussion Initially our attention was directed to some aspects of the biosynthesis of intracellular sucrose isomerase by Serratia plymuthica ATCC 598. The data presented on Figure shows, that the enzyme is synthesized along with the biomass until the 8 h of the development of the cells. After that its production was maintained at the same level until the 48 h and decreases after further cultivation. On the basis of this results were immobilized S. plymuthica cells in different growth phases of their development. The immobilized preparations, which were obtained from cell cultivated for -4 h had the highest specific enzyme activity (Figure ). It is interesting, that the specific activity of the cells cultivated for 4 h was lower. This is probably due to some structural changes in the cell wall, which hinder the diffusion of the substrate to the enzyme. In order to increase the specific activity of the immobilized in alginate preparations they were placed in liquid nutrient medium with the same composition and in the same conditions as those during the
3 Conversion of Sucrose into Palatinose with Immobilized Serratia Plymuthica Cells Biomass, g/l (ww) Time, h Biomass, g/l (ww) Activity, U/g (ww) Activity, mg (Palatinose)/g(gel).min h h 4h 4h Activity of the immobilized cells Activity of the immobilized cells after Fig.. Activity of the immobilized preparations with different cell age Fig.. Microbial growth and biosynthesis of intracellular sucrose isomerase during the cultivation of Serratia plymuthica ATCC 598 cultivation of the free cells. Therefore, for 8 h of treatment the specific activity increased with 5-3% (Figure ). Further treatment led to the development of cells outside the gel, which was especially well exhibited during the immobilization of cells, cultivated for 4 h. These preparations showed the biggest increase of specific activity (resulting from the increase of the viable cell counts in the gel matrix), but they proved to be instable because of disintegration of the gel beads. With the obtained immobilized cells was conducted a successful conversion of sucrose (% solution) into palatinose in a batch reactor. The results are presented on Figure 3 and Figure 4. A dependency was determined between the conversion rate and the gel: substrate solution proportion (m/v). With the decrease of the quantity of the substrate solution in relation to the weight of the immobilized preparation a higher conversion rate could be achieved for a shorter reaction time. When this proportion was :, the gel beads were - mm in diameter, at the optimal temperature 4-43 C, for 6 min was reached 99% conversion rate (Figure 4). The described immobilized preparations demonstrated good operational stability. The activity of the immobilized cells
4 74 A. Krastanov, D. Blazheva and T. Yoshida :7 :4 :4 after 6h Conversion rate, % 45 4 Palatinose content, mg/ml Time, min 75 5 Fig. 3. Conversion of sucrose into palatinose with immobilized in alginate beads cells at different gel: substrate solution proportion, m/v Fig. 4. Conversion of sucrose into palatinose with immobilized in alginate beads cells at gel: substrate solution proportion (m/v) : (- 3 min reaction time; - 5 min reaction time) did not change significantly after 6 h of continuous conversion of sucrose into palatinose (Figure 5). The conversion of sucrose into palatinose with immobilized on the surface of chitin S. plymuthica cells was conducted in a continuous tubular reactor. A flow rate of ml/min (. column volumes/ h) provided simultaneously the best productivity and the highest content of palatinose in the reaction medium (Figure 6). The obtained conversion rate, palatinose content in the eluate respectively, was higher than the described in literature analogous systems, employing immobilized Erwinia rhapontici cells (Cheetham, 98). The influence of temperature and sucrose concentration on the conversion rate and the productivity of the column with immobilized on chitin S. plymuthica cells was investigated. Maximal productivity and conversion rate were detected at 4-43 o C. The conversion rate of the % sucrose solutions was almost two times bigger than that of the 4% solutions and consequently the concentration of the sucrose solutions had comparatively weak effect on the productivity of the column (Figure 7). A better stability
5 Conversion of Sucrose into Palatinose with Immobilized Serratia Plymuthica Cells Productivity, mg/min Palatinose content, mg/ml Activity, % Temperature, o C Flow rate, ml/min Fig. 5. Temperature profile of the immobilized in alginate Serratia plymuthica cells Fig. 6. Influence of the flow rate on the productivity of the column reactor and the palatinose content in the outlet solution after the immobilization Serratia plymuthica cells on chitin Conversion rate, % Productivity, g/l Temperature, o C % sucrose solution Temperature, o C % sucrose solution 4% sucrose solution 4% sucrose solution a b Fig. 7. Influence of the temperature on the conversion rate (a) and the productivity (b) of a column reactor with immobilized on chitin Serratia plymuthica cells
6 76 of the productivity was observed when the process was conducted with more concentrated sucrose solutions at temperatures higher than the optimal. Conclusions As a result of these experiments can be concluded, that the described preparations of immobilized in alginate and on chitin S. plymuthica cells can successfully be used for production of palatinose from concentrated sucrose solutions (up to 4%) in batch and continuous enzyme reactors. The immobilization methods are easy to execute and the produced preparations are stable and have high specific activity. This enables the realization of the conversion process at comparatively high temperatures, which given the specific character of the employed products, has a significant technological importance. Acknowledgement The National Science Fund, Bulgaria sponsored this work. References Adachi, S., 964. Thin-layer chromatography of carbohydrates in the presence of bi-sulfite. Journal of Chromatgraphy, 7: A. Krastanov, D. Blazheva and T. Yoshida Cheetham, P., C. E. Imber, and J. Isherwood, 98. The formation of isomaltulose by immobilized Erwinia rhapontici. Nature, 99: Cheetham, P., C. Garrett and J. Clark, 985. Isomaltulose production using immobilized cells. Biotechnology and Bioengineering, 7: Goda, T. and N. Hosoya, 983. Hydrolysis of palatinose by rat intestinal sucraseisomaltase complex. Journal of Japanese Society of Nutrition and Food Science, 36: McAllister, M, C. T. Kelly, E. Doyle and W. M. Fogarty, 99. The isomaltulose synthesizing enzyme of Serratia plymuthica. Biotechnology Letters, (9): Minami, T, T. Fujiwara, T. Ooshima, Y. Nakajima and S. Hamada, 99. Interaction of structural isomers of sucrose in the reaction between sucrose and glucosyltransferases from mutans streptococci. Oral Microbiology and Immunology, 5: Mitzutani, T., 99. Preparation and use of palatinose oligosaccharides. New Food Industry, 33: 9-. Veronese, T and P. Perlot, 999. Mechanism of sucrose conversion by the sucrose isomerase of Serratia plymuthica ATCC 598. Enzyme and Microbial Technology, 4: Received June,, 6; accepted September, 8, 6.
Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.9 No.5, Issue of October 15, 2006 2006 by Pontificia Universidad Católica de Valparaíso -- Chile Received December 28, 2005 / Accepted April 25,
More informationINVESTIGATION ON CONVERSION OF FLOWER WASTES INTO BIOETHANOL AND PERFORMANCE EVALUATION ON SINGLE CYLINDER IC ENGINE
INVESTIGATION ON CONVERSION OF FLOWER WASTES INTO BIOETHANOL AND PERFORMANCE EVALUATION ON SINGLE CYLINDER IC ENGINE COLLEGE : BAPUJI INSTITUTE OF ENGINEERING AND TECHNOLOGY, DAVANGERE DEPARTMENT : MECHANICAL
More informationBio-Surfactant Production by Pseudomonasaeruginosa ATCC 9027 and It s Application in Microbial Enhanced Oil Recovery
International Refereed Journal of Engineering and Science (IRJES) ISSN (Online) 2319-183X, (Print) 2319-1821 Volume 5, Issue 11 (November 2016), PP.50-54 Bio-Surfactant Production by Pseudomonasaeruginosa
More informationETHANOL PRODUCTION FROM YAM BEAN USING YEAST Saccharomyces cerevisiae TISTR 5339
ETHANOL PRODUCTION FROM YAM BEAN USING YEAST Saccharomyces cerevisiae TISTR 5339 Waesarat Soontornchaiboon 1, Orawan Chunhachart 2, Ratchapol Pawongrat 1,* 1 Bioproduct Sciences program, Department of
More informationOptimization of Corynebacterium glutamicum immobilization process on bacterial cellulose carrier and its application for lysine fermentation
IOSR Journal of Engineering (IOSRJEN) ISSN (e): 2250-3021, ISSN (p): 2278-8719 Vol. 04, Issue 07 (July. 2014), V4 PP 33-38 www.iosrjen.org Optimization of Corynebacterium glutamicum immobilization process
More informationOptimization of Bacillus Subtilis Natto Immobilization Process on Alginate Chitosan Complex and Its Application for Nattokinase Fermentation
International Journal of Pharmaceutical Science Invention ISSN (Online): 2319 6718, ISSN (Print): 2319 670X Volume 5 Issue 3 June 2016 PP.25-30 Optimization of Bacillus Subtilis Natto Immobilization Process
More informationPRODUCTION OF D-TAGATOSE, A NOVEL LOW CALORIES SWEETENER BY A NEW ACETIC ACID BACTERIUM, Asaia bogorensis NRIC 0311 T
PRODUCTION OF D-TAGATOSE, A NOVEL LOW CALORIES SWEETENER BY A NEW ACETIC ACID BACTERIUM, Asaia bogorensis NRIC 0311 T Chotirot Sudto 1, Duangtip Moonmangmee 1,* Somporn Moonmangmee 2, Osao Adachi 3 and
More informationThe optimization of fermentation conditions particularly physical and chemical
4.1 Preamble The optimization of fermentation conditions particularly physical and chemical parameters are of primary importance in the development of any fermentation process owing to their impact on
More informationFrom waste to fuel: bioconversion of domestic food wastes to energy carriers
From waste to fuel: bioconversion of domestic food wastes to energy carriers M. Alexandropoulou 1,2, N. Menis 1, G. Antonopoulou 2, I. Ntaikou 2, G. Lyberatos 1,2 1 School of Chemical Engineering, National
More informationCell Growth and DNA Extraction- Technion igem HS
Growing Cells and DNA Extraction Goals 1. Become familiar with the process of growing bacteria 2. Get to know the DNA extraction process 3. Perform miniprep in the lab Keywords 1. Growth stages 6. Techniques
More informationResearch Article Pullulan Production by Aureobasidiumpullulans ATCC Cells Adsorbed onto Cellulose Anion and Cation Exchangers
International Scholarly Research Network ISRN Microbiology Volume 2012, Article ID 140951, 4 pages doi:10.5402/2012/140951 Research Article Pullulan Production by Aureobasidiumpullulans ATCC 201253 Cells
More informationContinuous Ethanol Production with Immobilized Ca-Alginate Cells Technique in Packed Bed Bioreactor using Bacteria: Z. mobilis and Mutated Z.
Continuous Ethanol Production with Immobilized Ca-Alginate Cells Technique in Packed Bed Bioreactor using Bacteria: Z. mobilis and Mutated Z.mobilis Raden Darmawan a*, Tri Widjaja a, Tontowi Ismail a,
More informationBACTERIAL BIOFILMS FORMATION AT AIR LIQUID INTERFACES
Innovative Romanian Food Biotechnology Vol. 5, Issue of December, 009 009 by Dunărea de Jos University Galaţi Received September 1, 009/ Accepted November 8, 009 RESEARCH ARTICLE BACTERIAL BIOFILMS FORMATION
More informationHomework #3. From the textbook, problems 9.1, 9.2, 9.3, 9.10, In 9.2 use q P = 0.02 g P / g cell h.
Homework #3 From the textbook, problems 9.1, 9.2, 9.3, 9.10, 9.15 In 9.2 use q P = 0.02 g P / g cell h. In 9.10 the factor k s is k d, the kinetic factor for the cell death. Also, use r=0 for part (b)
More informationBioreactors and Fermenters. Biometrix Corporation (800)
Bioreactors and Fermenters Biometrix Corporation (800)-890-89 1 Course Objectives This lesson will discuss bioreactors including basic operations, typical instrumentation configurations and calibration
More informationKluyveromyces Marxianus Biofilm in Cheese Whey Fermentation for Bioethanol Production
Kluyveromyces Marxianus Biofilm in Cheese Whey Fermentation for Bioethanol Production Yogesh Joshi, Beatrice Senatore, Massimo Poletto Dipartimento di Ingegneria Chimica e Alimentare, University of Salerno,
More informationA COMPARATIVE STUDY OF KINETIC IMMOBILIZED YEAST PARAMETERS IN BATCH FERMENTATION PROCESSES
European Journal of Advanced Research in Biological and Life Sciences Vol. No., 7 ISSN -98 A COMPARATIVE STUDY OF KINETIC IMMOBILIZED YEAST PARAMETERS IN BATCH FERMENTATION PROCESSES Terkida Prifti (Vaso)
More informationSOME ASPECTS OF CARBOHYDRATE METABOLISM AND PRODUCTION OF GLYCOSYLTRANSFERASES FROM MUTANT STRAIN LEUCONOSTOC MESENTEROIDES M2860
SOME ASPECTS OF CARBOHYDRATE METABOLISM AND PRODUCTION OF GLYCOSYLTRANSFERASES FROM MUTANT STRAIN LEUCONOSTOC MESENTEROIDES M2860 T. Vasileva 1, V. Bivolarski 1, I. Ivanova 2 and I. Iliev 1 1 Plovdiv University,
More informationUsage of food industry by-products as raw materials in lactic acid fermentation
Usage of food industry by-products as raw materials in lactic acid fermentation Jelena Pejin 1, Miloš Radosavljević 1, Milana Pribić 1, Sunčica Kocić-Tanackov 1, Dragana Mladenović 2, Aleksandra Djukić-Vuković
More informationLARGE SCALE PRODUCTION OF LACCASE BY PLEUROTUS OSTREATUS IMI IN BIOREACTOR
LARGE SCALE PRODUCTION OF LACCASE BY PLEUROTUS OSTREATUS IMI 395545 IN BIOREACTOR ABSTRACT In this chapter large scale laccase production from Pleurotus ostreatus IMI 395545 in a bench top bioreactor was
More informationIMMOBILIZATION OF CATALASE BY CROSS-LINKING OF PERMEABILIZED YEAST CELLS IN A PROTEIN SUPPORT
IMMOBILIZATION OF CATALASE BY CROSS-LINKING OF PERMEABILIZED YEAST CELLS IN A PROTEIN SUPPORT B.S. Kubal and S.F. D Souza Nuclear Agriculture and Biotechnology Division Bhabha Atomic Research Centre This
More informationUsing household food waste as a source of energy in a single-chamber microbial fuel cell
Using household food waste as a source of energy in a single-chamber microbial fuel cell Antonopoulou G. 1,2, Ntaikou I. 1,2, Alexandropoulou M. 1,2, Tremouli A. 1, Pastore C. 3, Bitonto L. 3, Bebelis
More informationA STUDY ON DENITRIFICATION IN A FLUIDIZED BED BIOREACTOR
Refereed Proceedings The 13th International Conference on Fluidization - New Paradigm in Fluidization Engineering Engineering Conferences International Year 2010 A STUDY ON DENITRIFICATION IN A FLUIDIZED
More informationGENERALIZED NET FOR CARBON DIOXIDE MONITORING OF FERMENTATION PROCESSES. Olympia Roeva, Tania Pencheva, Stoyan Tzonkov
Годишник на секция Информатика Съюз на учените в България Том 1, 2008, 93-97 Annual of Informatics Section Union of Scientists in Bulgaria Volume 1, 2008, 93-97 GENERALIZED NET FOR CARBON DIOXIDE MONITORING
More informationCourse Title. Master of Science Program in Industrial Biotechnology (International Program)
เอกสารแนบ ๑๐ Course Title Master of Science Program in Industrial Biotechnology (International Program) Master Degree: Master of Science Program in Industrial Biotechnology (International Program) Academic
More informationA novel approach to Protein A capture chromatography to help decrease cost of clinical manufacturing and reduce supply chain risk
PRODUCT BULLETIN POROS MabCapture A resins A novel approach to Protein A capture chromatography to help decrease cost of clinical manufacturing and reduce supply chain risk Introduction As cell culture
More informationENGINEERING ESCHERICHIA COLI FOR BIOFUEL PRODUCTION
ENGINEERING ESCHERICHIA COLI FOR BIOFUEL PRODUCTION Benchmarking with Butanol in Microbes Kajan Srirangan, Lamees Akawi, Lyndia Stacey, Cheryl Newton, Perry Chou and Marc Aucoin The Clostridium acetobutylicum
More informationFuture Perspectives of Antibody Manufacturing
BioProduction 2005 Amsterdam Future Perspectives of Antibody Manufacturing John Birch Lonza Biologics Monoclonal Antibodies A Success Story Fastest growing segment of the pharmaceutical market Sales forecast
More informationAppendix. Medium Composition. Peptone - 0.5gm (gram) Yeast extract - 0.5gm. Beef extract - 0.1gm. NaCl - 0.5g. Agar - 2gm. ph Starch - 0.
Appendix Medium Composition Nutrient Agar Peptone - 0.5gm (gram) Yeast extract - 0.5gm Beef extract - 0.1gm NaCl - 0.5g Agar - 2gm Distilled water - 100ml ph - 7.0 Starch Agar Starch - 0.5 Peptone - 0.5
More informationPHEN 612 SPRING 2008 WEEK 4 LAURENT SIMON
PHEN 612 SPRING 2008 WEEK 4 LAURENT SIMON Bioreactors Breads, yogurt, cheeses, etc Recombinant DNA techniques are used to make cheese. Fermentation is a microbial process that is used to produce food products
More informationSanitization and endotoxin clearance in AxiChrom columns
Application note 8-990-4 AE Chromatography columns Sanitization and endotoxin clearance in AxiChrom columns Columns used in cgmp environments need to maintain very high standards of hygiene. AxiChrom chromatography
More informationBiotechnology : Unlocking the Mysterious of Life Seungwook Kim Chem. & Bio. Eng.
Biotechnology : Unlocking the Mysterious of Life 2004 Seungwook Kim Chem. & Bio. Eng. Biotechnology in movies Biotechnology is An area of applied bioscience and technology which involves the practical
More informationEffect of glucose and ammonium chloride supplementation and phosphate buffer on Escherichia coli DH5α growth in LB Lennox medium
Effect of glucose and ammonium chloride supplementation and phosphate buffer on Escherichia coli DH5α growth in LB Lennox medium Wenfa Ng Department of Chemical and Biomolecular Engineering, National University
More informationProduction and Optimization of α-amylase by Bacillus licheniformis ATCC 6346 in Lab Bench-Scale Fermenter
Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res.,, ():9- (http://scholarsresearchlibrary.com/archive.html) ISSN : 3 368 CODEN (USA) : JMBRB4 Production
More informationSyamsul Falah Suryani Azmi Azhari. Department of Biochemistry Faculty of Matemathics and Natural Sciences Bogor Agricultural University
Bioethanol Production from Falcata (Paraserianthes falcataria) Wood by Enzymatic Delignification and Simultaneous Saccharification Fermentation using Immobilized Cells Syamsul Falah Suryani Azmi Azhari
More informationEnzymes. 13. Explain the active site theory to examine enzyme function
Name: 2.2 Cell Metabolism Objectives At the end of this sub section students should be able to: 2.2.1 Metabolism 1. Define the term: metabolism. 2.2.2 Sources of energy 2. State that solar energy is source
More informationVALORIZATION OF MAIZE SILAGE AND MUNICIPAL WASTEWATER SLUDGES: ENERGY PRODUCTION ENHANCED BY ULTRASONIC AND MICROWAVE PRE-TREATMENTS
VALORIZATION OF MAIZE SILAGE AND MUNICIPAL WASTEWATER SLUDGES: ENERGY PRODUCTION ENHANCED BY ULTRASONIC AND MICROWAVE PRE-TREATMENTS B. Aylin ALAGÖZ Prof. Dr. Orhan YENİGÜN Prof. Dr. Ayşen ERDİNÇLER Boğaziçi
More informationENTRAPMENT OF BACILLUS LICHENIFORMIS CELLS IN ALGINATE GEL FOR PROTEOLYTIC ENZYMES PRODUCTION
Lucrări ştiinńifice Zootehnie şi Biotehnologii, vol. 41(1) (2008), Timişoara ENTRAPMENT OF BACILLUS LICHENIFORMIS CELLS IN ALGINATE GEL FOR PROTEOLYTIC ENZYMES PRODUCTION ENTRAPAREA CELULELOR DE BACILLUS
More informationEffect of the start-up length on the biological nutrient removal process
Water Pollution IX 521 Effect of the start-up length on the biological nutrient removal process F. J. Fernández 1, J. Villaseñor 1 & L. Rodríguez 2 1 Department of Chemical Engineering, ITQUIMA, University
More informationPhysiological Characterization of a Bacillus licheniformis Strain in Chemostat Cultivations
Physiological Characterization of a Bacillus licheniformis Strain in Chemostat Cultivations Carla Pinto Department of Chemical Engineering, Lund University, Sweden June 11, 2012 Abstract The aim of the
More informationProduction of Bacillus licheniformis ATCC alkaline protease in batch, repeated batch and continuous culture
Malaysian Journal of Microbiology, Vol 6(2) 2010, pp. 156-160 Production of Bacillus licheniformis ATCC 21415 alkaline protease in batch, repeated batch and continuous culture Samia A. Ahmed * and Ahmed
More informationCapture of mouse monoclonal antibodies
Capture of mouse monoclonal antibodies by cation exchange chromatography Life Science Products Processing Capture of mouse monoclonal antibodies from cell culture supernatant by cation exchange chromatography
More informationProduction and purification of enzyme Xylanase by Aspergillus niger
ISSN: 2319-7706 Volume 3 Number 10 (2014) pp. 664-668 http://www.ijcmas.com Original Research Article Production and purification of enzyme Xylanase by Aspergillus niger S.Subbulakshmi and Priya R Iyer*
More informationEnzymatic synthesis of levan polysaccharide by Bacillus licheniformis levansucrase. Imen Dahech*, Rania Bredai, Karima Srih
ISSN : 0974-7427 Volume 8 Issue 5 Enzymatic synthesis of levan polysaccharide by Bacillus licheniformis levansucrase Imen Dahech*, Rania Bredai, Karima Srih Biochemistry laboratory, Faculty of sciences
More informationImmobilized Cells of Recombinant Escherichia coli Strain for Continuous Production of L-aspartic Acid
Polish Journal of Microbiology 2011, Vol. 60, No 2, 105 112 ORGINAL PAPER Immobilized Cells of Recombinant Escherichia coli Strain for Continuous Production of L-aspartic Acid GRAŻYNA SZYMAŃSKA*, BOGUSŁAW
More informationContents. Preface XI Nomenclature XIII. Part I Basic Concepts and Principles 1
V Preface XI Nomenclature XIII Part I Basic Concepts and Principles 1 1 Introduction 3 1.1 Background and Scope 3 1.2 Dimensions and Units 4 1.3 Intensive and Extensive Properties 6 1.4 Equilibria and
More informationFig. 5.1(a) and Fig. 5.1(b), on page 3 of the insert, show some changes that take place in the fermenter over the first 6 days.
1 A batch fermenter is used during the production of beer. Fig. 5.1(a) and Fig. 5.1(b), on page 3 of the insert, show some changes that take place in the fermenter over the first 6 days. (a) (i) Describe
More informationPetrifilm RYM. Interpretation Guide. Rapid Yeast and Mould Count Plate. Brand
Petrifilm Brand Interpretation Guide The 3M Petrifilm Rapid Yeast and Mould Count Plate is a sample-ready culture medium system that contains nutrients supplemented with antibiotics, a cold-water-soluble
More informationProduction and Purification of Lactase of Kluyveromyces Fragilis from Whey
Arch. Imt. RAZI (1998) 48-49 Production and Purification of Lactase of Kluyveromyces Fragilis from Whey Golcbin far F., Madani R., Youseti J.V. and Safavieb S. Biotechnology Deparunent Razi Vaccine & Serum
More informationOperating Instructions Heparin Mimetic Affinity
DexS Cellufine MAX DexS is a new chromatography resin incorporating Dextran sulfate polymer surface modification. This non-animal derived pseudo affinity ligand can be used instead of immobilized Heparin
More informationApplication of green fluorescent protein signal for effective monitoring of fermentation processes
Available online atwwwscholarsresearchlibrarycom Archives of Applied Science Research, 214, 6 (6):17-111 (http://scholarsresearchlibrarycom/archivehtml) ISSN 975-58X CODEN (USA) AASRC9 Application of green
More informationGlutathione Agarose Resin User s Guide
Glutathione Agarose Resin User s Guide DESCRIPTION Glutathione Agarose Resin is used to purify recombinant derivatives of glutathione S-transferases or glutathione binding proteins. Resins are products
More information2.4 TYPES OF MICROBIAL CULTURE
2.4 TYPES OF MICROBIAL CULTURE Microbial culture processes can be carried out in different ways. There are three models of fermentation used in industrial applications: batch, continuous and fed batch
More informationOptimization of deproteinization modes of chitine-containing raw materials in producing chitosane from micellar mushrooms of Aspergillus species
Academia Journal of Biotechnology 6(2): 042-046, February 208 DOI: 0.543/ajb.207.04 ISSN 235-7747 208 Academia Publishing Research Paper Optimization of deproteinization modes of chitine-containing raw
More informationLaboratory experiments of lead biosorption by self-immobilized Rhizopus nigricans pellets in the batch stirred tank reactor and the packed bed column
A. KOGEJ and A. PAVKO, Lead Biosorption, Chem. Biochem. Eng. Q. 15 (2) 75 79 (2001) 75 Laboratory experiments of lead biosorption by self-immobilized Rhizopus nigricans pellets in the batch stirred tank
More informationProduction of Biofuels and Value-Added Products
Metabolically engineered microbial systems and the conversion of agricultural biomass into simple sugars Microbial for the production Systems of biofuels For and The valueadded products Production of Biofuels
More informationBIOTECHNOLOGY. Course Syllabus. Section A: Engineering Mathematics. Subject Code: BT. Course Structure. Engineering Mathematics. General Biotechnology
BIOTECHNOLOGY Subject Code: BT Course Structure Sections/Units Section A Section B Unit 1 Unit 2 Unit 3 Unit 4 Unit 5 Unit 6 Unit 7 Section C Section D Section E Topics Engineering Mathematics General
More informationOptimization of Fermentation processes Both at the Process and Cellular Levels. K. V. Venkatesh
Optimization of Fermentation processes Both at the Process and Cellular Levels 'Simultaneous saccharification and fermentation of starch to lactic acid' K. V. Venkatesh Department of Chemical Engineering
More informationExtraction of enzymes from activated sludge
Waste Management and the Environment IV 249 Extraction of enzymes from activated sludge D. Nabarlatz 1, J. Vondrysova 2, P. Jenicek 2, F. Stüber 1, J. Font 1, A. Fortuny 3, A. Fabregat 1 & C. Bengoa 1
More informationAcknowledgement - 7th World Congress of Chemical Engineering Abstract Title (max 150 char): Abstract Text (max 2500 char): Topic
7th World Congress of Chemical Engineering Acknowledgement - 7th World Congress of Chemical Engineering 59338 Family Name First Name Title (Prof, Dr, Mr, Mrs, Ms) Institution/Company Department Address
More informationBioreactor System ERT 314. Sidang /2011
Bioreactor System ERT 314 Sidang 1 2010/2011 Chapter 2:Types of Bioreactors Week 2 Choosing the Cultivation Method The Choice of Bioreactor Affects Many Aspects of Bioprocessing. Product concentration
More informationАquaphotomics in biotechnology - new tools and opportunities
Аquaphotomics in biotechnology - new tools and opportunities Prof. Albert Krastanov UNIVERSITY OF FOOD TECHNOLOGIES AQUAPHOTOMICS time-efficient allows rapid, chemical-free, noninvasive in vivo assessment
More informationChapter 3 SCREENING AND SELECTION OF STRAIN FOR ALKALINE PROTEASE PRODUCTION BY SUBMERGED FERMENTATION
Chapter 3 SCREENING AND SELECTION OF STRAIN FOR ALKALINE PROTEASE PRODUCTION BY SUBMERGED FERMENTATION - 42 - 3.1 MATERIAL AND METHODS 3.1.1 Isolation of bacterial strains for alkaline protease production
More informationPolyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762
Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762 Wenfa Ng Department of Chemical and Biomolecular Engineering, National University of Singapore Abstract Email: ngwenfa771@hotmail.com
More informationMolecular characterization and phylogenetic analysis of protease producing Streptomyces sp. isolated from mangrove sediments
Molecular characterization and phylogenetic analysis of protease producing Streptomyces sp. isolated from mangrove sediments M. Parthasarathy and J. Joel Gnanadoss* Department of Plant Biology and Biotechnology,
More informationProduction of Cellulase on Mixtures of Xylose and Cellulose in a Fed-Batch Process
Production of Cellulase on Mixtures of Xylose and Cellulose in a Fed-Batch Process Ali Mohagheghi, Karel Grohmann, and Charles E. Wyman Biotechnolog y Research Branch, Solar Fuels Research Division, Solar
More informationDetermination of the kinetic parameters of batch fermentation of Lactobacillus paracasei RN5 with probiotic potential
Determination of the kinetic parameters of batch fermentation of Lactobacillus paracasei RN5 with probiotic potential Rositsa Denkova, Bogdan Goranov, Zapryana Denkova, Georgi Kostov, Ivanka Georgieva
More informationSTUDIES ON THE EFFECTS OF END PRODUCT INHIBITION OVER LACTIC ACID BACTERIA UNDER HIGH CELL DENSITY CULTIVATION PROCESS
Int. J. Chem. Sci.: 8(5), 21, S92-S99 STUDIES ON THE EFFECTS OF END PRODUCT INHIBITION OVER LACTIC ACID BACTERIA UNDER HIGH CELL DENSITY CULTIVATION PROCESS G. SREEKUMAR *, SOUNDARAJAN KRISHNAN a and R.
More informationAFFINITY GST PURIFICATION
DESCRIPTION Glutathione Agarose Resin is used to purify recombinant derivatives of glutathione S-transferases or glutathione binding proteins. are products that allow batch or column purifications. Purification
More informationFormation of Reducing Sugars from Sucrose by Erwinia Species
Journal of Genercrl Microbiology (I 975), 88, 36 737 I Printed ii7 Great Britain 367 Formation of Reducing Sugars from Sucrose by Erwinia Species By BARBARA M. LUND Agricultural Research Council Food Research
More informationOptimization of ethanol production from cheese whey powder by Kluyveromyces fragilis using factorial design and response surface methodology
Proceedings of the 10th International Chemical and Biological Engineering Conference - CHEMPOR 008 Braga, Portugal, September 4-6, 008 E.C. Ferreira and M. Mota (Eds.) Optimization of ethanol production
More informationContinuous bioremediation of phenol polluted air in an external loop airlift bioreactor with packed bed Hossein Nikakhtari 1 and Gordon A.
CONTINUOUS BIOREMEDIATION OF PHENOL POLLUTED AIR IN AN EXTERNAL LOOP AIRLIFT BIOREACTOR 211 Continuous bioremediation of phenol polluted air in an external loop airlift bioreactor with packed bed Hossein
More informationChapter 5. Alginate affinity purification of PG
Chapter 5 Alginate affinity purification of PG 1 5.0 Significance and focus of the work IMP improved the specific activity of PG from SmF culture broth but did not improve the specific activity of SSF-PG
More informationINFLUENCE OF CULTIVATION MEDIA ON HALOBACTERIA I. GROWTH AND BIOMASS FORMATION
INFLUENCE OF CULTIVATION MEDIA ON HALOBACTERIA I. GROWTH AND BIOMASS FORMATION MIRONESCU Monica *, MIRONESCU Ion Dan *, JÂŞCANU Vasile *, POSTEN Clemens ** * Lucian Blaga University of Sibiu *University
More information5 th Semester Syllabus
5 th Semester Syllabus NAME: COURSE: B.Sc. COLLEGE: IQ Education Roll #: Class Room: DNA Typing, Proteomics & Beyond (DPB-501) 1. DNA Typing DNA polymorphisms: the basis of DNA typing, Minisatellite analysis,
More informationAFFINITY GST PURIFICATION
DESCRIPTION Glutathione Agarose Resin is used to purify recombinant derivatives of glutathione S-transferases or glutathione binding proteins. are products that allow batch or column purifications. Purification
More informationINFLUENCE OF DIFFERENT CONDITIONS FOR BIOFILM FORMATION ON THE OXIDATION ACTIVITY OF ACIDITHIOBACILLUS FERROOXIDANS
INFLUENCE OF DIFFERENT CONDITIONS FOR BIOFILM FORMATION ON THE OXIDATION ACTIVITY OF ACIDITHIOBACILLUS FERROOXIDANS V. Mamatarkova Sofia University St. Kliment Ohridski, Faculty of Biology, Department
More informationPROTEIN L AGAROSE BEADS
DESCRIPTION Protein L is an immunoglobulin-binding protein that was isolated from the bacteria Peptostreptococcus magnus and provides a convenient way to separate immunoglobulins from a variety of sources.
More information4.4 MICROBIOLOGICAL METHOD FOR THE ESTIMATION OF. The microbiological assay was performed by using the test
109 4.4 MICROBIOLOGICAL METHOD FOR THE ESTIMATION OF AMOXICILLIN The microbiological assay was performed by using the test organism Staphylococcus aureus. The strain was isolated from soil and allowed
More informationImmobilization of a Thermostable á-amylase on Calcium Alginate Beads from Bacillus Subtilis KIBGE-HAR
Australian Journal of Basic and Applied Sciences, 3(3): 2883-2887, 2009 ISSN 1991-8178 Immobilization of a Thermostable á-amylase on Calcium Alginate Beads from Bacillus Subtilis KIBGE-HAR 1 2 1 1 Aliya
More informationSimultaneous saccharification and fermentation of Arundo donax - Comparison of feeding strategies
Simultaneous saccharification and fermentation of Arundo donax - Comparison of feeding strategies Bhargav Prasad Kodaganti Abstract Department of Chemical Engineering, Lund University, Sweden September,
More informationCell free xanthan gum production using continuous recycled packed fibrous-bed bioreactor-membrane
Malaysian Journal of Microbiology, Vol () 008, pp. - Cell free xanthan gum production using continuous recycled packed fibrous-bed bioreactor-membrane Rosalam S *. Krishnaiah D. and Bono A. Advanced membrane
More informationBabyBio S BabyBio Q BabyBio DEAE
DATA SHEET BabyBio S BabyBio Q BabyBio DEAE BabyBio S, BabyBio Q and BabyBio DEAE are pre-packed, ready to use ion exchange columns for easy and convenient purification of proteins. Rapid method screening
More informationIncrease of Xylitol Production Rate by Controlling Redox Potential in Candida parapsilosis
Increase of Xylitol Production Rate by Controlling Redox Potential in Candida parapsilosis Deok-Kun Oh, 1 Sang-Yong Kim, 2 Jung-Hoe Kim 3 1 Department of Food Science and Technology, Woosuk University,
More informationInt. J. Pharm. Sci. Rev. Res., 32(2), May June 2015; Article No. 26, Pages:
Research Article Enhanced Production of Cellulase on Different Fruit Peel Under Submerged Fermentation M. Kannahi*, S. Elangeswari PG and Research Department of Microbiology, Sengamala Thayaar Educational
More informationLactic acid fermentation of a combined agro-food waste substrate
Lactic acid fermentation of a combined agro-food waste substrate Ljiljana Mojović *, Aleksandra Djukić-Vuković*, Dragana Mladenović *, Jelena Pejin** * University of Belgrade, Faculty of Technology and
More informationA Discovery Laboratory Investigating Bacterial Gene Regulation
Chapter 8 A Discovery Laboratory Investigating Bacterial Gene Regulation Robert Moss Wofford College 429 N. Church Street Spartanburg, SC 29307 mosssre@wofford.edu Bob Moss is an Associate Professor of
More informationMOISTURE REQUIREMENTS OF BACTERIA
II. MOISTURE REQUIREMENTS OF BACTERIA INFLUENCE OF TEMPERATURE, ph, AND MALATE CONCENTRATION ON REQUIREMENTS OF Aerobacter aerogenes' R. J. WODZINSKI2 AND W. C. FRAZIER Department of Bacteriology, University
More informationPRODUCTION, CHARACTERIZATION AND PURIFICATION FROM
PRODUCTION, CHARACTERIZATION AND PURIFICATION OF XYLANASE FROM FROM Staphylococcus aureus MBXi-K4 Indah Wijayanti Bogor Agricultural University 1 INTRODUCTION ABSORBTION OF NUTRIENT PERFORMANCE OF POULTRY
More informationInvertase Assay Kit. Catalog Number KA assays Version: 03. Intended for research use only.
Invertase Assay Kit Catalog Number KA1629 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General
More informationEffect of microbial activity on dimethyl sulfoxide degradation in an air-lift bioreactor
Waste Management and the Environment VI 43 Effect of microbial activity on dimethyl sulfoxide degradation in an air-lift bioreactor S.-C. J. Hwang 1, Y.-H. Lin 2, Y.-C. Chang 1 & S.-Y. He 1 1 Department
More informationQUESTIONSHEET 1. The diagram shows a method of screening fungi for the production of an antibiotic. fungus A fungus B fungus C [2] ...
QUESTIONSHEET 1 The diagram shows a method of screening fungi for the production of an antibiotic. test fungus petri dish containing nutrient agar 1 2 3 4 5 6 streaks of different test bacteria The diagrams
More informationEffects of Oxygen Supply and Mixed Sugar Concentration on D-Ribose Production by a Transketolase-Deficient Bacillus subtilis SPK1
J. Microbiol. Biotechnol. (2013), 23(4), 560 564 http://dx.doi.org/10.4014/jmb.1212.12021 First published online January 27, 2013 pissn 1017-7825 eissn 1738-8872 Effects of Oxygen Supply and Mixed Sugar
More informationProduction of cellulase by submerged and solid-state cultures and yeasts selection for conversion of lignocellulose to ethanol
Romanian Biotechnological Letters Vol. 14, No. 2, 2009, pp. 4275-4281 Copyright 2009 Bucharest University Printed in Romania. All rights reserved Romanian Society of Biological Sciences ORIGINAL PAPER
More informationContinuous Xylose Fermentation by Candida shehatae in a Two-Stage Reactor
In: Scott, Charles D., ed. Proceedings of the 9th symposium on biotechnology for fuels and chemicals; 1987 May 5-8; Boulder, CO. In: Applied Biochemistry and Biotechnology. Clifton, NJ: Humana Press; 1988:
More informationPOTENTIAL LIPID PRODUCTION OF OLEAGINOUS YEAST LIPOMYCES STARKEYI FROM GLUCOSE AND XYLOSE
POTENTIAL LIPID PRODUCTION OF OLEAGINOUS YEAST LIPOMYCES STARKEYI FROM GLUCOSE AND XYLOSE Noppan Peawsuphon, Anusith Thanapimmetha, Maythee Saisriyoot, Penjit Srinophakun * *Department of chemical Engineering,
More informationProduction of Ethanol by Fed-Batch Fermentation
Pertanika J. Sci. & Technol. 17 (2): 399 408 (2009) ISSN: 0128-7680 Universiti Putra Malaysia Press Production of Ethanol by Fed-Batch Fermentation Ngoh Gek Cheng 1*, Masitah Hasan 1, Andri Chahyo Kumoro
More informationImprovement of fermentative production of exopolysaccharides from Aureobasidium pullulans under various conditions
Korean J. Chem. Eng., 31(8), 1433-1437 (2014) DOI: 10.1007/s11814-014-0064-9 INVITED REVIEW PAPER INVITED REVIEW PAPER pissn: 0256-1115 eissn: 1975-7220 Improvement of fermentative production of exopolysaccharides
More informationSimulation of a reactor for glucose isomerization to fructose by immobilized glucose isomerase with continuous enzyme renewal
Biotechnology Techniques, Vol, No 2, February 997, pp. 99 03 Simulation of a reactor for glucose isomerization to fructose by immobilized glucose isomerase with continuous enzyme renewal A. Converti*,
More information