Rheological and biochemical analyses on blood coagulation ~ Discovery of a new pathway under stagnant flow conditions ~
|
|
- Mervin Powers
- 6 years ago
- Views:
Transcription
1 Rheological and biochemical analyses on blood coagulation ~ Discovery of a new pathway under stagnant flow conditions ~ Computer and Information Division, RIKEN Hiroki Iwata Supramolecular Science Laboratory, RIKEN Makoto Kaibara Biomolecular Characterization Division, RIKEN Naoshi Dohmae Koji Takio
2 tissue factor VIIa Ca 2+ Extrinsic system Gelation reaction XII XI IX XIIa X XIa IXa Xa Intrinsic system prothrombin thrombin XIII XIIIa Ca 2+ fibrinogen (gelation) fibrin (crosslinks) fibrin clot fibrinolysis
3 Stasis Erythrocyte aggregation High hematocrit Factor IX? ein thrombosis Blood coagulation Erythrocyte Factor IXa Vein thrombosis Activation of coagulation factor IX on erythrocyte membrane
4 Time of onset of coagulation of blood sample Blood sample Whole blood PFP PRP PFP + Granulocytes PFP + Erythrocytes Cell number (cells/ l) Platelets < 100 Platelets < 1-40 x 10 4 (Leukocytes x 10 3 ) Granulocytes x 10 3 Erythrocytes < 5 x x 10 6 > 4 x 10 6 Coagulation time (min) not coagulated not coagulated S.Kawakami, et al.: Biorheology, 32(5): , 1995
5 Coagulation of coagulation factor-deficient PFP supplemented with erythrocytes PFP sample PFP (normal) Factor VII-, XI or XII-deficient Factor IX-, VIII- or X-deficient Coagulation time (min) not coagulated S.Kawakami, et al.: Biorheology, 32(5): , 1995
6 Factor XI Factor IX Factor X SDS-PAGE analysis of activation of coagulation factors by erythrocyte membrane
7 Purification Procedure Erythrocytes Dissolved with detergent Anion exchange chromatography Heparin-affinity chromatography SBTI-affinity chromatography A extract (single band in SDS-PAGE analysis) A complex with 1-protease inhibitor Determination of amino acid sequence
8 Affinity chromatography on heparin sepharose column (3) (1) (4) (5) A: 50mM TrisHCl B: A + 0.8M NaCl (1) (2) (3) (4) (5) Factor IX Factor IXa
9 Purified by SBTI-affinity chromatography (kda) (1)(2) complex 1-PI (1) Neutrophil elastase (2) Purified enzyme Complex formation with 1-protease inhibitor (1) (2) (3) (4) (5) (6) (7) 1-PI concentratio (1) non added (2) 0.40ppm (3) 0.80ppm (4) 1.60ppm (5) 3.20ppm (6) 6.40ppm (7) 12.8ppm Analysis of N-terminal sequence
10 N-Terminal amino acid sequence of factor IX-activating enzyme on erythrocyte membrane Amino acid sequence of human leukocyte elastase Underlined: the observed amino acid sequence
11 Sites of Factor IX cleaved by the extract
12 Hematocrit (%) F-IX F-IXa Effect of hematocrit on activation of factor IX
13 sec -1 Viscosity (mpa sec) sec -1 Coagulation Time (min) Time (min) Shear rate (sec -1 ) Effect of flow shear rate on coagulation time
14 0.6 sec sec -1 F-IX F-IXa Time (min) Time (min) Effect of flow shear rate on activation of factor IX
15 Logarithmic damping factor (LDF) normal diabetes Time (min) F-IX F-IXa diabetes normal Time (min) Time (min) Coagulation time and enzymatic activity of erythrocyte from different donors
16 oagulation reaction Factor IX Activation Enzymatic activity Factor XIa Erythrocyte elastase hemical reaction Ala-Ala-Pro-Val-MCA Enzymatic activity Ala-Ala-Pro-Val AMC Fluorescence
17 Observation of erythrocytes labeled with elastase-specific fluorogenic substrate
18 tissue factor VIIa Ca 2+ Extrinsic system Gelation reaction stasis rythrocyte aggregation high hematocrit IX X IXa Xa prothrombin thrombin XIII XIIIa Ca 2+ fibrinogen (gelation) fibrin (crosslinks) fibrin clot XI XIa XII XIIa Intrinsic system fibrinolysis
19 Conclusion 1. Factor IX is activated by enzyme on erythrocyte membrane. The enzymes are homologous to leukocyte elastase, The enzyme activates Factor IX by cleaving several bonds which sites are close to the bonds cleaved by Factor XIa. 2. Identification of Factor IX activating enzyme on erythrocyte membrane could provide a frame work for defining roles in cardiovascular coagulation and for refining strategies in pharmaceutical development.
Coagulation Mechanisms Dr. Nervana Bayoumy
Coagulation Mechanisms Dr. Nervana Bayoumy Associate Professor Department of Physiology Objectives At the end of this lecture you should be able to: 1. Recognize the different clotting factors 2. Understand
More informationGeneral Principles of. Hemostasis. Kristine Krafts, M.D.
General Principles of Hemostasis Kristine Krafts, M.D. Hemostasis is a balancing act! pro-ting plugs up holes in blood vessels anti-ting keeps ting under control Pro-Clotting Pro-Clotting vessels platelets
More informationIntroduction Hemostasis: Tourniquet Test & Bleeding Time. Hematology-Immunology System Faculty of Medicine Universitas Padjadjaran LOGO
Introduction Hemostasis: Tourniquet Test & Bleeding Time Hematology-Immunology System Faculty of Medicine Universitas Padjadjaran LOGO Hemostasis A series of reactions that function to stop bleeding, maintaining
More informationCoagulation Cascade. TF-VIIa. Tissue Factor + VII. XIIa. Prothrombin. XIa. IXa. VIIIa. Thrombin. XL- Fibrin. XIII Monomer. Fibrinogen.
Coagulation Cascade Intrinsic Pathway Extrinsic Pathway Contact Activation XIIa XI XIa Prekallikrein HMW Kininogen IX IXa Ca 2+ Anticoagulation proteins: Protein C, Protein S, Antithrombin III, TFPI TF
More informationSerine Proteases and their Inhibitors
ugo Kubinyi, www.kubinyi.de Serine Proteases and their Inhibitors ugo Kubinyi Germany E-Mail kubinyi@t-online.de omepage www.kubinyi.de ugo Kubinyi, www.kubinyi.de Serine Proteases of Physiological Importance
More informationCoagulation in perspective: Blood management. Objectives
Coagulation in perspective: Blood management Julie Wegner, PhD jawrbl@gmail.com Objectives To gain a basic understanding of the following: 1. Coagulation components and processes Why patients bleed. 2.
More informationThese handouts are only meant as a guide while you follow the presentation on the screen. Sometimes the speaker will change some of the slides.
These handouts are only meant as a guide while you follow the presentation on the screen. Sometimes the speaker will change some of the slides. If you would like the 1 slide per page handouts, please ask
More informationEnzyme Research Laboratories product list 2018
Milan Analytica AG Baslerstrasse 15 4310 Rheinfelden Switzerland www.milananalytica.ch Enzyme Research Laboratories product list 2018 Product # Sizes/quantity Human Proteins Human Prothrombin Prothrombin
More informationEvaluation of Complex Coagulation Cases: Case-Based Illustrations of Important Issues
Evaluation of Complex Coagulation Cases: Case-Based Illustrations of Important Issues Kristi J. Smock, MD Associate Professor of Pathology University of Utah Health Sciences Center Medical Director, Hemostasis/Thrombosis
More informationSign up to receive ATOTW weekly -
BLOOD PHYSIOLOGY PART 2 ANAESTHESIA TUTORIAL OF THE WEEK 231 11 TH JULY 2011 Dr Karen Hayes Royal Devon & Exeter Correspondence to: kmhayes@hotmail.co.uk QUESTIONS Before continuing, try to answer the
More informationThe Role Of Point of Care Coagulation Testing (POCCT) in Patient Blood Management. Elham Khalaf Adeli
The Role Of Point of Care Coagulation Testing (POCCT) in Patient Blood Management Elham Khalaf Adeli Overview PBM, bleeding management, Coagulation POC Testing Review of laboratory conventional coagulation
More informationBLOOD COAGULATION. 1. The initial phase of the process is vascular constriction. This limits the flow of blood to the area of injury.
BLOOD COAGULATION The ability of the body to control the flow of blood following vascular injury is paramount to continued survival. The process of blood clotting and then the subsequent dissolution of
More informationEvolution of the Clotting Cascade. Kalyan Banda MD Hematology Fellows Conference 01/13/2017
Evolution of the Clotting Cascade Kalyan Banda MD Hematology Fellows Conference 01/13/2017 Who am I? Born in St. Petersburg, Russia Trivial injuries led to life threatening bleeds all of my life Usually
More informationUsing TEG in the ED, OR, and ICU. Don H. Van Boerum, MD, FACS
Using TEG in the ED, OR, and ICU Don H. Van Boerum, MD, FACS Trauma Surgeon, Director of Surgical Critical Care, Co-Director of Shock Trauma ICU, Intermountain Medical Center, Intermountain Healthcare
More informationPlatelet Factor IV- Heparin Antibodies. Presenter: Michael J. Warhol, M.D.
Platelet Factor IV- Heparin Antibodies Presenter: Michael J. Warhol, M.D. Learning Objectives Describe the mechanism of interaction between Heparin and Platelet Factor 4 Review the chemistry of Heparin
More informationCHROMOGENIC SUBSTRATE TECHNOLOGY. Giovanni Russi
CHROMOGENIC SUBSTRATE TECHNOLOGY Giovanni Russi What is a chromogenic substrate? A peptide linked to a chromophore The peptide is formed by 3-5 residues The chromophore is p-nitroaniline (p-na) The residues
More informationMethods of Biomaterials Testing. Special Biology: Blood & Vessel
Methods of Biomaterials Testing Special Biology: Blood & Vessel Blood Compatibility Mainly: Inhibition of blood clot formation Materials in Bloodflow Venous catheter Dialysis filter Dialysis shunt Heart
More informationWhy measure thrombin generation? How does measurement of thrombin generation work? Sample material / pre-analytics Test performance Applications
Why measure thrombin generation? How does measurement of thrombin generation work? Sample material / pre-analytics Test performance Applications Revision V02/2009 Theory of coagulation, 1905 thromboplastin
More informationSpecial Coagulation - APC Resistance. DiaPharma Group, Inc. Customer Service: Technical Support:
Special Coagulation - APC Resistance DiaPharma Group, Inc. Customer Service: 1.800.526.5224 Technical Support:1.800.447.3846 www.diapharma.com 1 Review of Hemostasis Overview Pathways of coagulation, anticoagulation,
More informationA MANUFACTURING PROCESS TO OBTAIN A HIGHLY-PURIFIED TRIPLE-SECURED FIBRINOGEN PRODUCT
A MANUFACTURING PROCESS TO OBTAIN A HIGHLY-PURIFIED TRIPLE-SECURED FIBRINOGEN PRODUCT Maduell P, Domingo N, López L, Gensana M, Caballero S, Belda P, Gajardo R, Grancha S, Ristol P R&D Area, Instituto
More informationLABORATORY APPROACH TO BLEEDING DISORDERS DR NISHANTH PG 1 ST YEAR DEPARTMENT OF PATHOLOGY
LABORATORY APPROACH TO BLEEDING DISORDERS DR NISHANTH PG 1 ST YEAR DEPARTMENT OF PATHOLOGY 1 WHEN IS THE LAB REQUIRED TO INVESTIGATE FOR A POSSIBLE BLEEDING DISORDER? Clinically suspected bleeding tendency
More informationBasic coagulation applications and case studies
Basic coagulation applications and case studies Jing Jin Clinical laboratory Scientist (MLS, ASCP) - Coagulation/Hematology Stanford University Hospital and Clinics 1 Agenda Overview about 3 major phases
More informationAdv Pathophysiology Unit 4 Page 1 of 10. Learning Objectives for this file:
Adv Pathophysiology Unit 4 Page 1 of 10 Learning Objectives for this file: Topics include structure & function of: 1. Coagulation process platelets, clotting factors 2. Coagulation cascade formation of
More informationLaboratory investigation in the Bleeding Patient. Dr Craig Taylor Consultant Haematologist May 2016
Laboratory investigation in the Bleeding Patient Dr Craig Taylor Consultant Haematologist May 2016 Introduction Bleeding is common May consume significant resources Crossmatched blood Lab results may be
More informationHaemostasis. The function of haemostasis is: to prevent blood loss from injured vessels. to stop bleeding. to prevent thrombosis
Haemostasis Haemostasis The function of haemostasis is: to prevent blood loss from injured vessels to stop bleeding to prevent thrombosis Haemostasis blood endothelium basement membrane subendothelium
More informationHemostasis. by Mohie-Aldien Elsayed
Hemostasis by Mohie-Aldien Elsayed Hemostasis Hemostasis is defined as the process that maintains the flowing blood in a fluid state and confined to the circulatory system Coagulation prevents excess blood
More informationPlasma Testing in the Clinical Coagulation Laboratory: New drugs, new problems.
Test Plasma Testing in the Clinical Coagulation Laboratory: New drugs, new problems. Karen A. Moffat BEd, MSc, ART, FCSMLS(D) Technical Specialist, Coagulation, HRLMP Assistant Professor, Department of
More informationLina Al-Lawama. Rama Al-Ashqar. Malik Al-Zohlof
2 Lina Al-Lawama Rama Al-Ashqar Malik Al-Zohlof Anticoagulant drugs Recap Last lecture we were talking about antiplatelet drugs, we mentioned 5 drugs : Aspirin which is used alone with patents that have
More informationManaging the Risks Associated with Anticoagulant Therapy. Steve McGlynn Specialist Principal Pharmacist (Cardiology) NHS Greater Glasgow and Clyde
Managing the Risks Associated with Anticoagulant Therapy Steve McGlynn Specialist Principal Pharmacist (Cardiology) NHS Greater Glasgow and Clyde Background Identified as high risk medicines Wide range
More informationImmunomodulation at Cardiac Surgery. William T McBride MD
Immunomodulation at Cardiac Surgery William T McBride MD March 2005 Basic Structure of Immune System Immune response Adaptive specific memory Innate non-specific no memory ADAPTIVE (specific) b INNATE
More informationTable of Contents. Introduction. Worldwide Market and Technology Overview
Table of Contents Introduction Worldwide Market and Technology Overview A. Major Routine and Special Coagulation Tests 1. Introduction 2. Activated Partial Thromboplastin Time (APTT) 3. Alpha-2 Antiplasmin
More informationLinking in silico and in vitro experiments to identify and evaluate a biomarker for enoxaparin activity
Linking in silico and in vitro experiments to identify and evaluate a biomarker for enoxaparin activity Abhishek Gulati What is enoxaparin? Low molecular weight heparin anticoagulant Used to minimise the
More informationCh 13: Blood. What does blood do? Transport: Regulation: Protection:
Ch 13: Blood What does blood do? Transport: Regulation: Protection: SLOs Describe the composition of plasma and list the major functions of plasma proteins. Map hematopoiesis, starting from a pluripotent
More informationP. W. HOWIE M.D., M.R.C.O.G. first method of activation is the intrinsic system. Hageman factor, which in turn activates a series of
Postgraduate Medical Journal (May 1979) 55, 362-366 Blood clotting and fibrinolysis in pregnancy P. W. HOWIE M.D., M.R.C.O.G. MRC Unit of Reproductive Biology, Edinburgh Summary During normal pregnancy,
More informationF11 (Human) ELISA Kit
F11 (Human) ELISA Kit Catalog Number KA1400 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General Information...
More informationPhysiology Unit 3 HEMATOLOGY
Physiology Unit 3 HEMATOLOGY In Physiology Today Hematocrit Percentage of blood volume that is erythrocytes 45% in men 42% in women Average blood volume in is 5 L Plasma Large amounts of organic/inorganic
More informationSensoLyte Rh110 Factor Xa Assay Kit *Fluorimetric*
SensoLyte Rh110 Factor Xa Assay Kit *Fluorimetric* Catalog # 72207 Kit Size 100 Assays (96-well plate) Optimized Performance: This kit is optimized to detect Factor Xa enzyme activity Enhanced Value: It
More informationCurrent laboratory practices in the diagnosis and management of haemophilia
Current laboratory practices in the diagnosis and management of haemophilia Marc Jacquemin University of Leuven BSTH 2013 Detection of severe haemophilia A Validation of the FVIII assay for the detection
More informationProduct Data Sheet - TRUEMAB
888.267.4436 techsupport@origene.com www.origene.com Name:F13A1 (Factor XIIIa) mouse monoclonal antibody, clone OTI3E3 (formerly 3E3) Product Data Sheet - TRUEMAB Catalog: TA800346 Components: F13A1 (Factor
More informationImproving Outcomes of Cardiac Surgery with a Point-of-Care Based Transfusion Algorithm
Improving Outcomes of Cardiac Surgery with a Point-of-Care Based Transfusion Algorithm Summer Syed (local PI) Keyvan Karkouti Deputy Anesthesiologist-in-Chief, Toronto General Hospital, University Health
More informationCoagulation Laboratory: Methods, Standards & Cost Effective Testing Part 1
Coagulation Laboratory: Methods, Standards & Cost Effective Testing Part 1 Donna D. Castellone, MS, MT (ASCP) SH Clinical Project Manager Hematology and Hemostasis Siemens Healthcare Diagnostics Disclosures
More informationThe Cardiovascular System: Blood
The Cardiovascular System: Blood The Functions of Blood General Overview Provides a system for rapid transport within the body Nutrients Hormones Waste products Respiratory gases Cells & Blood Components
More informationSensoLyte Rh110 Factor Xa Assay Kit *Fluorimetric*
SensoLyte Rh110 Factor Xa Assay Kit *Fluorimetric* Revision Number: 1.1 Last updated: October 2014 Catalog # Kit Size AS-72207 100 Assays (96-well plate) Optimized Performance: This kit is optimized to
More informationProduct Data Sheet - TRUEMAB
888.267.4436 techsupport@origene.com www.origene.com Name:F13A1 (Factor XIIIa) mouse monoclonal antibody, clone OTI1H2 (formerly 1H2) Product Data Sheet - TRUEMAB Catalog: TA800305 Components: F13A1 (Factor
More informationLecture 8: Affinity Chromatography-III
Lecture 8: Affinity Chromatography-III Key words: Chromatography; Affinity chromatography; Protein Purification During this lecture, we shall be studying few more examples of affinity chromatography. The
More informationLVHN Scholarly Works. Lehigh Valley Health Network. Joseph G. Ottinger RPh, MS, MBA, BCPS Lehigh Valley Health Network,
Lehigh Valley Health Network LVHN Scholarly Works Department of Pharmacy Retrospective Evaluation of Delayed Administration of Fondaparinux in Providing Comparable Safety and Efficacy Outcomes in Patients
More informationInhibition of Plasmin Generation in Plasma by Heparin, Low Molecular Weight Heparin. and a Covalent Antithrombin-Heparin Complex (ATH)
Inhibition of Plasmin Generation in Plasma by Heparin, Low Molecular Weight Heparin and a Covalent Antithrombin-Heparin Complex (ATH) Gabriela Chang, H. BSc A Thesis Submitted to the School of Graduate
More informationActivation and specificity of Thrombin. Giulia Pavani
Activation and specificity of Thrombin Giulia Pavani Summary Regulation of a Serine Protease: Thrombin Zymogen Enzyme Substrate Specificity Staphylocoagulase Bacteria know how a protease works (much more
More informationDisclosures. I have received research funding from: I have received consulting fees/honoraria from: Novo Nordisk CSL-Behring Boehringer Ingelheim
Test Disclosures I have received research funding from: Novo Nordisk CSL-Behring Boehringer Ingelheim I have received consulting fees/honoraria from: Novo Nordisk CSL-Behring Baxter The Medicines Company
More informationHaematologY. Dr Tom Bate Again!
HaematologY Dr Tom Bate Again! THE FRCA What you need to know Blood Groups ~30 blood group systems recognised (ISBT) ABO, Rhesus, Lewis, Kell, Duffy, Kidd MNS system, Kell system The ABO antigens are the
More informationChanges in coagulation and tissue plasminogen activator after the treatment of cerebral infarction with lumbrokinase
Clinical Hemorheology and Microcirculation 23 (2000) 213 218 213 IOS Press Changes in coagulation and tissue plasminogen activator after the treatment of cerebral infarction with lumbrokinase Lirong Jin
More informationLaboratory Investigation of Challenging Cases. Laura A. Worfolk, Ph.D Scientific Director, Coagulation
Laboratory Investigation of Challenging Cases Laura A. Worfolk, Ph.D Scientific Director, Coagulation Coagulation Cascade XII XIIa XI HMWK/Prekallikrein XIa VII Injury IX X IXa VIII Xa X TF TF/VIIa Prothrombin
More informationTransfusion & Non-transfusion Approaches to Bleeding
Transfusion & Non-transfusion Approaches to Bleeding Maureane Hoffman, MD, PhD Professor of Pathology, Duke University and Director, Transfusion Service and Hematology Laboratory Durham Veterans Affairs
More informationOERMATAN SULFATE: A NEW CONCEPT IN ANTITHROMBOTIC THERAPY
OERMATAN SULFATE: A NEW CONCEPT IN ANTITHROMBOTIC THERAPY by JOANNE VAN RYN-McKENNA, B.Sc., M.Sc. A Thesis Submitted to the School of Graduate Studies in Partial Fulfilment of the Requirements for the
More informationChromogenic and Fluorogenic Substrates
Chromogenic and Fluorogenic Substrates for enzymes involved in Haemostasis Chromogenic and Fluorogenic Substrates The PEFACHROME and PEFAFLUOR line of chromogenic and fluorogenic peptide substrates is
More informationWestmead Hospital XENOTRANSPLANTATION Making Pigs Fly
Westmead Hospital XENOTRANSPLANTATION Making Pigs Fly Philip O Connell Mismatch Between Islet Donors and Recipients 150,000 190 45 There are not enough donors Why Pig as a Donor Few Ethical Problems Potential
More informationSonoclot Analyzer User Guide
Sonoclot Analyzer User Guide for Cardiopulmonary Bypass Surgery Rev 1. Sienco, Inc. 5721 Arapahoe Ave, Unit A1-A, Boulder, CO 833 USA 33-42-1148 1-8-432-1624 Fax 33-379-443 www.sienco.com e-mail: sienco@sienco.com
More informationAssayMax Human Factor XI ELISA Kit
AssayMax Human Factor XI ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing
More information*NCCT is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. Program, provider #122.
COURSE DESCRIPTION Have you ever wondered why the blood that a phlebotomist collects in a tube will form a clot unless certain chemicals are added to the tube in advance to prevent it? Have you ever wondered
More informationSysmex Educational Enhancement and Development No
SEED Haematology Sysmex Educational Enhancement and Development No 3 2014 The Thrombin Time test and Reptilase test what is their role in coagulation testing? The purpose of this newsletter is to provide
More informationCommon Inherited Bleeding Disorders
CAA 2015 Annual Conference Common Inherited Bleeding Disorders Bob Miller, A October 8, 2015 VWF has two jobs Loosely bound to protect FVIII and tether to site of injury All other coagulation factors also
More informationIntroduction to Protein Purification
Introduction to Protein Purification 1 Day 1) Introduction to Protein Purification. Input for Purification Protocol Development - Guidelines for Protein Purification Day 2) Sample Preparation before Chromatography
More informationCOMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Thrombin Generation Assay. Kit Insert
COMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Thrombin Generation Assay Kit Insert Version: February 2018 Summary Thrombin is a key enzyme of the coagulation cascade. Its measurement
More informationCritical Quality Attributes for Blood Products. Tim Lee, Ph.D. OBRR/CBER/FDA 12 January 2009 WCBP
Critical Quality Attributes for Blood Products Tim Lee, Ph.D. OBRR/CBER/FDA 12 January 2009 WCBP 1 Disclaimer The views expressed during this presentation are my own and may not be those of the FDA 2 Blood
More informationPXBioVisioN. Protease Inhibition. Analysis of Peptides as Surrogates for Protease Activity to Monitor Protease Inhibition in-vivo
PXBioVisioN Protease Inhibition Analysis of Peptides as Surrogates for Protease Activity to Monitor Protease Inhibition in-vivo Peptides are Products of Protease Activity Peptidomics is defined as the
More informationLocal vasoconstriction. is due to local spasm of the smooth muscle (symp. reflex) can be maintained by platelet vasoconstrictors
Hemostasis Hemostasis ( hemo =blood; sta= remain ) is the stoppage of bleeding, which is vitally important when blood vessels are damaged. Following an injury to blood vessels several actions may help
More informationHematology Emergencies: Problems with Platelets
Hematology Emergencies: Problems with Platelets Christian Cable, MD, FACP Associate Professor of Medicine Division of Hematology & Oncology Texas A&M HSC College of Medicine Scott & White Healthcare Fundamentals
More informationEffect of Fibrinogen and Ca2+ on the Thrombin-Catalyzed Proteolytic Event That Triggers Activation of Factor XI11
Effect of Fibrinogen and Ca2+ on the Thrombin-Catalyzed Proteolytic Event That Triggers Activation of Factor XI11 JULES A. SHAFER,' SIDNEY D. LEWIS," TODD J. JAN US,^ AND LASZLO LOR AND^ 'Department of
More informationAssessing Higher Order Structure and Comparability of Protein Therapeutics
Assessing Higher Order Structure and Comparability of Protein Therapeutics a Regulator s Perspective Evi Struble, PhD Pharmacologist CBER/OBRR/DH 1 Disclaimer This presentation reflects the views of the
More informationAn Analysis of the Robustness and Fragility of the Coagulation System
Complex Adaptive Systems Resilience, Robustness, and Evolvability: Papers from the AAAI Fall Symposium (FS-10-03) An Analysis of the Robustness and Fragility of the Coagulation System Nathan Menke MD PhD
More informationPharmacology Lecture 5. Anticoagulants
Pharmacology Lecture 5 Anticoagulants General overview Anti-thrombotic Drugs Antiplatlets Anticoagulants Fibrinolytics Anticoagulants Indirect Thrombin Inhibitors Anti-thrombotic effect is exerted by interaction
More informationISIS PHARMACEUTICALS. ISIS-FXI Rx Program Update. Webcast December 8, 2014
ISIS PHARMACEUTICALS ISIS-FXI Rx Program Update Webcast December 8, 2014 Introduction Stan Crooke, M.D., Ph.D. CEO and Chairman, Isis Pharmaceuticals 2 Forward Looking Language Statement This presentation
More information73 Coagulation Laboratory: Methods, Standards and Cost Effective Testing (Part 1) Donna Castellone MS, MT(ASCP)SH
73 Coagulation Laboratory: Methods, Standards and Cost Effective Testing (Part 1) Donna Castellone MS, MT(ASCP)SH 2011 Annual Meeting Las Vegas, NV AMERICAN SOCIETY FOR CLINICAL PATHOLOGY 33 W. Monroe,
More informationProtein analysis. Dr. Mamoun Ahram Summer semester, Resources This lecture Campbell and Farrell s Biochemistry, Chapters 5
Protein analysis Dr. Mamoun Ahram Summer semester, 2015-2016 Resources This lecture Campbell and Farrell s Biochemistry, Chapters 5 Bases of protein separation Proteins can be purified on the basis Solubility
More informationUMass Memorial Medical Center Worcester, MA
Poster #22 UMass Memorial Medical Center Worcester, MA Vascular Surgery Cardiac Catheterization Interventional Radiology Cardiac Surgery Heparin Therapy in Clinical Practice Heparin Therapy Excessive or
More informationPrimary hemostasis. Vascular endothelium Vasoconstriction : local tissue factor, nervous system
Primary hemostasis Vascular endothelium Vasoconstriction : local tissue factor, nervous system Platelet Plug Platelet Adhesion Platelet Activation Platelet Aggregation Platelet Plug Formation Secondary
More informationSensoLyte 520 Factor Xa Assay Kit *Fluorimetric*
SensoLyte 520 Factor Xa Assay Kit *Fluorimetric* Catalog # 72208 Kit Size 100 Assays (96-well plate) Optimized Performance: This kit is optimized to detect Factor Xa enzyme activity Enhanced Value: It
More informationANTITHROMBIN FACTOR Xa
2014 ATITRMBI FACTR Xa The Coamatic Antithrombin kits are simple, accurate and convenient. All methods are based on inhibition. Allows accurate determination of antithrombin in patients receiving heparin
More informationClumsy Coagulation Communication
Clumsy Coagulation Communication Let s Blame the Lab! Lab Clinician Communication Barriers and opportunities Where are the errors made? How do we enhance patient experience? George A Fritsma MS, MLS, Your
More informationCase 7 A Storage Protein From Seeds of Brassica nigra is a Serine Protease Inhibitor Last modified 29 September 2005
Case 7 A Storage Protein From Seeds of Brassica nigra is a Serine Protease Inhibitor Last modified 9 September 005 Focus concept Purification of a novel seed storage protein allows sequence analysis and
More informationCharacterization of antithrombin-specific RNA aptamers for use in anticoagulant therapy
Characterization of antithrombin-specific RNA aptamers for use in anticoagulant therapy Yolanda M. Fortenberry, Ph.D. Hematology 2014 Conference Johns Hopkins University School of Medicine What are aptamers?
More informationReverse the New Anticoagulants? Mitchell J Daley, PharmD, BCPS
Reverse the New Anticoagulants? Mitchell J Daley, PharmD, BCPS Clinical Pharmacy Specialist, Critical Care University Medical Center Brackenridge / Dell Seton Medical Center at the University of Texas
More informationLaboratory Investigation of Challenging Cases. Laura A. Worfolk, Ph.D Scientific Director, Coagulation
Laboratory Investigation of Challenging Cases Laura A. Worfolk, Ph.D Scientific Director, Coagulation Disclosures Employee of Quest Diagnostics Case focus: Importance of knowing test limitation/interpretations
More informationPurification of (recombinant) proteins. Pekka Lappalainen, Institute of Biotechnology, University of Helsinki
Purification of (recombinant) proteins Pekka Lappalainen, Institute of Biotechnology, University of Helsinki Physical properties of proteins that can be applied for purification -size -charge (isoelectric
More informationPurification: Step 1. Lecture 11 Protein and Peptide Chemistry. Cells: Break them open! Crude Extract
Purification: Step 1 Lecture 11 Protein and Peptide Chemistry Cells: Break them open! Crude Extract Total contents of cell Margaret A. Daugherty Fall 2003 Big Problem: Crude extract is not the natural
More informationPurification: Step 1. Protein and Peptide Chemistry. Lecture 11. Big Problem: Crude extract is not the natural environment. Cells: Break them open!
Lecture 11 Protein and Peptide Chemistry Margaret A. Daugherty Fall 2003 Purification: Step 1 Cells: Break them open! Crude Extract Total contents of cell Big Problem: Crude extract is not the natural
More informationResearch Article Massive Exploration of Perturbed Conditions of the Blood Coagulation Cascade through GPU Parallelization
BioMed Research International, Article ID 863298, 20 pages http://dx.doi.org/10.1155/2014/863298 Research Article Massive Exploration of Perturbed Conditions of the Blood Coagulation Cascade through GPU
More informationUvA-DARE (Digital Academic Repository) Factor XI as target for antithrombotic therapy van Montfoort, M.L. Link to publication
UvA-DARE (Digital Academic Repository) Factor XI as target for antithrombotic therapy van Montfoort, M.L. Link to publication Citation for published version (APA): van Montfoort, M. L. (2014). Factor XI
More informationCase 7 A Storage Protein From Seeds of Brassica nigra is a Serine Protease Inhibitor
Case 7 A Storage Protein From Seeds of Brassica nigra is a Serine Protease Inhibitor Focus concept Purification of a novel seed storage protein allows sequence analysis and determination of the protein
More informationLaboratory Monitoring of Anticoagulation
Michael Smith, Pharm. D., BCPS, CACP East Region Pharmacy Clinical Manager Hartford HealthCare Learning Objectives Explain the role of common laboratory tests used in monitoring of anticoagulation therapy.
More informationMECHANICAL AND LYTIC STABILITY OF THE FIBRIN MESH MODIFIED WITH VESSEL WALL COMPONENTS. Ph.D. Thesis ZSOLT ROTTENBERGER
MECHANICAL AND LYTIC STABILITY OF THE FIBRIN MESH MODIFIED WITH VESSEL WALL COMPONENTS Ph.D. Thesis ZSOLT ROTTENBERGER Molecular Medicine Doctoral School Semmelweis University Supervisor: Dr. Kraszimir
More informationPrimePCR Assay Validation Report
Gene Information Gene Name Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID coagulation factor XIII, A1 polypeptide F13A1 Human This gene encodes the coagulation
More informationSuitability of analytical methods for detection of thrombogenic factor XIa in Privigen
Suitability of analytical methods for detection of thrombogenic factor XIa in Privigen Absence of thrombogenic factors in Privigen M. Moses, K. Ruhwedel, H.-A. Stöhr, L. Duse, A. Feussner, W. Wormsbächer,
More informationHaemostasis Antibodies
HAEMOSTASIS ANTIBODIES FROM CEDARLANE www.cedarlanelabs.com NEW! The study of haemostasis and thrombosis has become one of the most rapidly growing fields in medical research. An unprecedented amount of
More informationThe Coagulation Workup In The Office Setting
CAA Conference alm Springs 2012 The Coagulation Workup In The Office Setting CAA Conference alm Springs, October 2012 Robert Miller, A-C Henoch-Schoenlein urpura 1 CAA Conference alm Springs 2012 2 CAA
More informationHaemostasis Antibodies
HAEMOSTASIS ANTIBODIES FROM CEDARLANE www.cedarlanelabs.com NEW! The study of haemostasis and thrombosis has become one of the most rapidly growing fields in medical research. An unprecedented amount of
More informationHemostasis and Thrombosis
Hemostasis Hemostasis and Thrombosis Normal hemostasis is a consequence of tightly regulated processes that maintain blood in a fluid state in normal vessels, yet also permit the rapid formation of a hemostatic
More informationExtracting Pure Proteins from Cells
Extracting Pure Proteins from Cells 0 Purification techniques focus mainly on size & charge 0 The first step is homogenization (grinding, Potter Elvejhem homogenizer, sonication, freezing and thawing,
More informationR.Li, C.Swaelens, F.Vandermijnsbrugge, B.Cantinieaux BSTH Laboratory of haematology, Porte de Hal,
Institut J. Bordet Normal with Actin-FS avoids intrinsic pathway factors assays in the presence of an isolated prolongation of (Platelin-LS) without hemorrhagic history R.Li, C.Swaelens, F.Vandermijnsbrugge,
More information