MICROBIOLOGY 20 (course #3124)

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1 Stephen Brown (instructor) Los Angeles Mission College, Fall 2008 Lab: TTh 5:30-6:55, INST Lecture: TTh 7:00-8:25, INST 2001 voic office hours: TTh 4:00-5:25 Bungalow B MICROBIOLOGY 20 (course #3124) Course Description: This course is an introduction to the fundamental principles of microbiology. The laboratory portion of the course covers microscopic and cultural techniques for studying and identifying microorganisms. Prerequisites: Successful completion of any of the following courses: required - Biology 3 or 6, AND Chemistry 51, 65 or 101 (or the equivalent) recommended English 28 (or the equivalent) Course Objectives: Upon completion of this course a successful student will be able to: Employ microbiology terminology correctly in the laboratory and workplace. Analyze the differences between prokaryotic and eukaryotic cells. List subcellular structures and organelles of prokaryotic and eukaryotic cells and their functions, draw the structure of prokaryotic and eukaryotic cells and label their parts. Compare and contrast different eukaryotic organisms including fungi, algae, and multicellular parasites using the compound light microscope, and describe important features. Describe the underlying mechanisms of chemical reactions in microbes, including energy and material flow. Compare and contrast the effect of environmental factors on microbial growth (ph, temperature, pressure, radiation, oxygen concentration, osmotic pressure, and nutrients). Differentiate important events of the four growth stages of bacterial populations (lag, log, stationary, and decline). Describe the processes of DNA replication, mutations, transcription, and translation. Distinguish among mechanisms of genetic recombination in bacteria (transformation, transduction, and conjugation). Organize and name the major taxonomic categories of bacteria, fungi, viruses, algae, protozoa, and helminths. Demonstrate knowledge of the different features of prokaryotes, their different taxonomical groupings and to use reference sources for microbial identifications. Analyze the traits of important microbiological eukaryotes and their clinical and environmental relevance.

2 Explain viral structure and the major steps in the life cycle of an animal virus. Describe the structure of a bacteriophage and distinguish between lytic and lysogenic life cycles. Examine and distinguish the stages of infectious disease (incubation, prodromal, illness, decline, and convalescence). Explain Koch s postulates for infectious diseases and exceptions to them. Distinguish between bacterial endotoxins and exotoxins and give examples of each. Describe microbial mechanisms to enter host, evade host defenses, and cause cellular damage. Explain the role of the nonspecific host defenses against microbial infection. Examine the features of the five categories of immunoglobulins (IgA, IgD, IgM, IgG, and IgE). Analyze specific host defenses against microbial infection, and distinguish between humoral and cell-mediated immunity. Discuss cells and cell fragments found in human blood and describe their functions in nonspecific and specific host responses. Distinguish among the major categories of immune disorders in humans, their signs and symptoms and the genetic and environmental factors that may play a role in the etiology of such diseases. Compare and contrast the major categories of antimicrobial drugs. Summarize the major diseases that affect different human organ systems due to specific microbial pathogens. Student Learning Outcomes: Evaluate and summarize a peer-reviewed article in microbiology or immunology and write a report using proper terminology. Identify an unknown bacterium using lab skills learned in the course. Required Books and Materials: BOOKS: Microbiology: An Introduction. Tortora, Funke, & Case, 9 th ed Pearson-Benjamin Cummings (ISBN ) Laboratory Experiments in Microbiology. Johnson & Case, 8 th ed Pearson-Benjamin Cummings (ISBN ) MATERIALS: bound notebook (graph ruled), Sharpie pen, colored pencil set 6 Scantron 882-E forms, 10 Scantron 815-E forms, 4 blue books (or Scantron 886-E) OTHER RESOURCES: (website for the course text) (Mission College department website)

3 Course Grade: Your Course Grade will be weighted as follows: 65% Lecture 35% Laboratory. 8 (of 10) Quizzes 12% of Grade (120 points) 2 (of 3) Exams 30% of Grade (300 points) Final Exam 18% of Grade (180 points) Article Review 5% of Grade (50 points) 2 Lab Exams 20% of Grade (200 points) Unknown Identifications 10% of Grade (100 points) Lab Notebook/Participation 5% of Grade (50 points) 1000 points total LECTURE: The lowest 2 quiz scores and lowest exam score will be dropped (final exam can NOT be dropped). The Final Exam will be comprehensive with approximately half of the questions relating to previous material. Exams and quizzes will consist of multiple choice, short answer and essay questions. Multiple choice questions are to be answered on SCANTRON forms. Short answer and essay questions are to be answered in Blue Books. Any make-up exams or quizzes will be short answer, essay ONLY. LAB: Students are expected to have read each lab exercise BEFORE class, and to be prepared to answer any lab report questions at the beginning of each lab. Completed lab notebook entries and questions are to be shown to the instructor at the end of each lab. Lab participation involves being present and doing the lab exercises, and completing a notebook entry for the day. Each student will receive scores reflecting the number of labs and lab entries completed (e.g., 80% completed = 40 pts). Grading Scale: 900+ pts (90-100%) A pts (78-89%) B pts (65-77%) C pts (55-64%) D pts (below 55%) F Attendance Policy: Attendance is required and roll will be taken. You are responsible for information, exam announcements, date changes, etc., presented in class, whether or not you are present. Students missing more than 2 consecutive classes may be dropped. Students given add slips must complete the process by Friday September 12 th. Students withdrawing from the class must do so by: Monday September 15 th to receive a refund Sunday September 28 th to avoid receiving a W on your transcript Sunday November 23 rd to receive a W and avoid a non-passing grade

4 Special Accommodations: If you require special accommodations, religious or ADA, inform me IN WRITING within the first two weeks of the semester. You will need to provide the appropriate documentation after which we will arrange to meet your needs. Cheating/Academic Dishonesty: Each student is expected to do his/her own work on all assignments, reports, examinations, etc. Cheating in any form will at a minimum result in ZERO points for the assignment or test, and may result in an "F" for the course. Here is a list of some actions that are considered cheating: Talking during an exam Copying answers from someone else's paper Using notes of any kind during an exam Showing a fellow student your exam or passing information Turning in someone else's work (plagiarism) Providing your work for someone else to copy Taking a call or text-message on your cell phone (please turn them off!) If you have a question during an exam, quietly walk up to the instructor and whisper your question. Translation dictionaries are not permitted during exams. Exiting the room during an exam is not permitted. Recommendations for succeeding in this Class: This is a demanding class covering a lot of information. Here are some suggestions: do NOT fall behind in the course, keep up with the material on a weekly basis each time you study, spend at least 10 minutes reviewing previous lessons (this is the secret to long term memory) read the relevant chapters in your textbook, hi-lite pertinent lines and add these notes to your class notes (always write as you read) use associations to help you remember things create flash cards and form study groups if you find that helpful know the key terms (you can t answer questions correctly if you don t!) at a minimum, you should learn the course material 3 times in order to retain it well for the exams and quizzes: 1) comprehend the class material during the lecture 2) read the corresponding material in the text while reviewing your notes 3) review your notes and key terms before the exams

5 LECTURE SCHEDULE (tentative) Week Date LECTURE TOPIC (textbook reading)* 1 Sep 2 Introduction, History of Microbiology (ch. 1) Review of Chemical Principles (ch. 2) Sep 4 Microbial Classification and Identification (ch. 10) Microscopy (ch. 3) 2 Sep 9 Eukaryotic, Prokaryotic Cell Structure & Function (ch. 4) Sep 11 QUIZ: Microbial Metabolism (ch. 5) 3 Sep 16 Microbial Growth (ch. 6) Sep 18 QUIZ: Bacterial Gene Transfer (ch. 8) 4 Sep 23 Survey of the Domains Bacteria & Archaea (ch. 11) Sep 25 EXAM #1 (chapters 3-6, 8, 10 & 11) 5 Sep 30 The Fungi (ch. 12, pp ) Oct 2 Parasitic Protozoa & Helminths (ch. 12, pp ) 6 Oct 7 QUIZ: Bacteriophage Viruses (ch. 13, pp ) Oct 9 Animal Viruses & Prions (ch. 13, pp ) 7 Oct 14 QUIZ: Control of Microbial Growth (ch. 7) Oct 16 Antimicrobial Drugs (ch. 20) 8 Oct 21 QUIZ: Microbial Mechanisms of Pathogenicity (ch. 15) Oct 23 EXAM #2 (chapters 7, 12, 13, 15 & 20) 9 Oct 28 Non-specific (Innate) Defenses of the Host (ch. 16) Oct 30 Adaptive Immunity: T cells & Antigen Presentation (ch. 17) 10 Nov 4 QUIZ: Cell-Mediated and Humoral Immune Responses (ch. 17) Nov 6 Vaccination & Diagnostic Immunology (ch. 18) 11 Nov 11 QUIZ: Disorders of the Immune System (ch. 19) Nov 13 Principals of Epidemiology (ch. 14) 12 Nov 18 QUIZ: Selected Bacterial Pathogens (ch ) Nov 20 Selected Bacterial Pathogens (cont d) (ch ) 13 Nov 25 EXAM #3 (chapters 14, 16-19, 21-26) Nov 27 THANKSGIVING HOLIDAY 14 Dec 2 Selected Viral Pathogens (ch ) Dec 4 QUIZ: Selected Eukaryotic Pathogens (ch ) 15 Dec 9 Environmental Microbiology (ch. 27, pp ) Dec 11 QUIZ: Microbiology in Foods & Industry (ch. 28) 16 Dec 18 Comprehensive FINAL EXAM * The lecture notes will be available through the department website ( in PDF format or by . If you prefer the notes to be sent to you on a weekly basis by , send an to me at <BrownST@lamission.edu> and ask to be put on the list for Micro 20. The sooner you do so, the sooner you ll get the notes!

6 LABORATORY SCHEDULE (tentative) Week Date LAB TOPIC EXERCISE 1 Sep 2 Lab Orientation Sep 4 Use and Care of the Microscope 1 2 Sep 9 Examination of Living Organisms 2 Sep 11 Preparation of Smears and Simple Staining 3 3 Sep 16 Gram Staining 5 Sep 18 Acid Fast Staining 6 4 Sep 23 Endospore Staining; Microbes in the Environment 7 & 9 Sep 25 Microbes in the Environment (cont); Aseptic Technique 9 & 10 5 Sep 30 Aseptic Technique (cont); Morphological Unknown given 10 & 8 Oct 2 Unknown (cont); Dilution & Streak Technique 8 & 11 6 Oct 7 Dilution & Streak (cont); Fungi Yeasts 11 & 34 Oct 9 Fungi Molds 35 7 Oct 14 Protozoa 37 Oct 16 Osmotic Pressure & ph 21 8 Oct 21 Osmotic Pressure & ph (cont); Growth Control Heat 21 & 22 Oct 23 Growth Control Heat (cont); Growth Control Chemicals 22 & 24 9 Oct 28 Growth Control Chemicals (cont); Biochemical Unknown given (analyze through Nov 13) Oct 30 Carbohydrate Catabolism Nov 4 Carbohydrate Catab. (cont); Fermentation of Carbohydrates 13 & 14 Nov 6 Fermentation (cont); Protein Catabolism I 14 & Nov 11 Protein Catabolism I (cont); Protein Catabolism II 15 & 16 Nov 13 Protein Catabolism II (cont); Respiration 16 &17 Complete Biochemical Unknown Identification 12 Nov 18 Respiration (cont); Rapid ID Methods; Microbes in Water 17, 18 & 52 Nov 20 Rapid ID Methods; Microbes in Water (cont); Antimicrobial Drugs 18 & Nov 25 Antimicrobial Drugs (cont); review for midterm 25 Nov 27 THANKSGIVING HOLIDAY Dec 2 LABORATORY MIDTERM EXAM Dec 4 Microbes in Food: Contamination Dec 9 Microbes in Food: Contamination (cont) 54 Dec 11 review for laboratory final exam Dec 16 LABORATORY FINAL EXAM

7 SCORE SHEET LECTURE POINTS LAB POINTS Quiz #1 Exam I Midterm Lab 1 Lab 21 Quiz #2 Exam II Lab Final Lab 2 Lab 22 Quiz #3 Exam III Unkn. #1 Lab 3 Lab 24 Quiz #4 Final Unkn. #2 Lab 5 Lab 13 Quiz #5 Article Lab 6 Lab 14 Quiz #6 Lab 7 Lab 15 Quiz #7 Lab 9 Lab 16 Quiz #8 Lab 10 Lab 17 Quiz #9 Lab 8 Lab 18 Quiz #10 Lab 11 Lab 52 Lab 34 Lab 25 Lab 35 Lab 54 Lab 37 LECTURE: Each lecture quiz will have 15 possible points (lowest score is dropped). Each of the first 3 exams will have 150 possible points (lowest score is dropped). The final exam will have 180 possible points. The article review will have 50 possible points (details will be provided in handout). LAB: Each lab exam will have 100 possible points. The morphological unknown identification (Unkn. #1) will have 30 possible points, and the biochemical unknown identification (Unkn. #2) will have 70 possible points (details will be provided in handout). You will receive 2 points for each lab and notebook entry completed. To keep track of your performance throughout the course, add up your total points earned and divide by the total possible points thus far in the class. Multiply by 100 to get a percent score to compare with the grade scale on page 3 of the syllabus.

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