Simultaneous multi-color, multiphoton fluorophore excitation using dual-color fiber lasers

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Simultaneous multi-color, multiphoton fluorophore excitation using dual-color fiber lasers"

Transcription

1 Multiphoton Microscopy / Fiber Laser Simultaneous multi-color, multiphoton fluorophore excitation using dual-color fiber lasers Matthias Handloser, Tim Paasch-Colberg, Bernhard Wolfring TOPTICA Photonics AG Advanced microscopy techniques provide crucial insights for a deeper understanding of biological and chemical processes. Due to its nonlinear excitation character, multiphoton imaging (e.g. TPEF, SHG, THG) enables a deeper penetration depth and higher spatial resolution than conventional techniques. Here we present a novel approach to two-photon excitation at various wavelengths simultaneously using TOPTICA s FemtoFiber dichro design biomp. The presented technique employs two femtosecond laser pulses at different wavelengths to stimulate a 2-color 2-photon excitation of GFP fluorophores inside the irradiated sample. This excitation corresponds to an excitation at a third, virtual wavelength. In a similar way the laser can excite most conventional fluorophores simultaneously. Fig. 1: TOPTICA S FemtoFiber dichro biomp fiber laser provides two synchronized femtosecond laser pulses at different wavelengths to enable 2-color 2-photon excitation. Introduction Multiphoton microscopy has become a popular method for cellular imaging. The nonlinear character of the fluorophore excitation results in an intrinsic three-dimensional resolution due to the high penetration depth, combined with a higher resolution compared to conventional techniques. For an efficient excitation of fluorescent labels, a pulsed laser source is required. Titanium-sapphire (Ti:sapph) lasers are frequently used due to their relatively reliable pulse parameters and broad wavelength tuning range. Covering about nm with ~100 fs pulses, Ti:sapph lasers enable excitation of most important fluorophores that are used for multiphoton microscopy. Nevertheless there are several disadvantages when using Ti:sapph lasers, especially when Page 1

2 several fluorophores that absorb light at different wavelengths are studied in one sample. In this case it is necessary to tune the wavelength of the laser for each particular fluorophore. In addition, Ti:sapphs are relatively large and require water cooling, as well as regular maintenance. Ultrafast fiber lasers for multiphoton microscopy Femtosecond fiber lasers are an alternative solution for fluorophore excitation in multiphoton microscopy. They are compact, reliable and maintenance-free sources that require no water cooling. They operate at a fixed wavelength, therefore they are the perfect choice for experiments that do not require a wavelength tunability a precondition that is quite often fulfilled, e.g. in two-photon excitation of Alexa 488 using a wavelength of 780 nm. 2-color 2-photon absorption using TOPTICA FemtoFiber dichro biomp To address several fluorophores simultaneously at different wavelengths with fiber lasers, another adapted excitation scheme must be employed. In conventional 2-photon excitation, two photons of identical wavelength are absorbed by the sample, e.g. 2*780 nm or 2*1040 nm, c.f. Fig. 2 (a) and (c). If femtosecond laser pulses of different wavelength overlap temporally and spatially in the laser focus at the sample, a process called 2-color 2-photon excitation can be stimulated. In that case, the sample absorbs two photons of different wavelength, e.g. 780 and 1040 nm, cf. Fig. 2 (b). This excitation corresponds to a conventional 2-photon excitation at a virtual wavelength of λvirt = 890 nm corresponding to the following equation: Fig. 2: Various pathways for two-photon excitation fluorescence (TPEF). On the one hand, two identical photons can be absorbed at different wavelengths each (a) and (c), alternatively absorption of two photons at different wavelengths can stimulate 2-color TPEF (b). TOPTICA s FemtoFiber dichro biomp (cf. Fig. 1) simultaneously provides two intrinsically synchronized laser beams with sub-150 fs pulses at different wavelengths of 780 and 1040 nm out of one box, even from the same aperture. The system includes a semiautomatic time-delay calibration, as well as an adjustable spatial beam-overlap (z-focus alignment) between both laser beams to guarantee for the spatial and temporal overlap of both beams at Page 2

3 one sample position to access the virtual wavelength created by photons of both beams. These features allow to cover all three excitation pathways described in Fig. 2 simultaneously, i.e. exciting fluorophores at nm is feasible without tuning the wavelength. For example GFP, YFP and Alexa fluorophores can all be excited at the same time, with acquisition times that are comparable to single channel imaging. Excitation of GFP-Neutrophil Cells To determine the feasibility of efficient 2-color 2-photon excitation of multiple fluorophores simultaneously, a TILL Photonics Intravital2P confocal microscope has been combined with a TOPTICA FemtoFiber dichro design biomp. The obtained confocal scan map is compared to the resulting image after standard 2- photon excitation by exciting the sample material with a Ti:sapph oscillator at the respective wavelength of 890 nm, serving as reference measurement. Both laser systems share the same optical beam path to allow for proper comparison of the retrieved 2- photon scan map. All images were recorded by appropriately spectral filtering of the 2- photon induced second harmonic signal generated by the investigated fluophores used for labelling the sample material throughout the experiment. A parasagittal section of a mouse brain s hippocampus was prepared as sample, whereas about 10 percent of the neurons express YFP, and neutrophil white blood cells express GFP. The neurons in the hippocampus are pyramidal neurons, which have large cell bodies and a long protrusion, the dendrite. Additionally, there are small protrusions originating from soma and dendrite. These form a dense mesh between the layer of cell bodies and the part that contains only dendrites. Dendrites run in parallel. Neutrophils crawl between cells and dendrites. They are small, have very short protrusions, if at all, and they are less brightly stained. They are most obvious in parts of the images dominated by pyramidal neuron dendrites. Page 3

4 Experiment Experimental results obtained by the described experimental procedure can be seen in Figs By exciting the investigated sample material at 780 nm only no 2-photon excited fluorescence (TPEF) signal is observed (Fig. 3), due to the mismatch of excitation and absorption spectral region. The green protein YFP, used for labelling the material, absorbs light from nm. Fig. 3: Microscopy signal recorded with FemtoFiber dichro design biomp at 780 nm only. No signal is observed since YFP absorbs in spectral range between nm. By exciting the investigated the sample material at a wavelength of 1040 nm only, corresponding to a two photon excitation of 520 nm, a strong TPEF signal is observed from the YFP linked to the neurons as depicted in Fig. 4. Fig. 4: FemtoFiber dichro design biomp at 1040 nm only, leading to 2-photon excited fluorescence of the YFP neurons. Page 4

5 Simultanous excitation of 780 nm and 1040 nm provided by the novel FemtoFiber dichro biomp leads to a strong TPEF signal as shown in Fig. 5. By setting the relative temporal delay of both beams to zero (built in motorized and controlled delay stage) and overlapping the excitation focii spatially (built in adjustment of z- focus position) the laser systems allows to excite the sample additionally at the virtual wavelength of 890 nm. This enables a controlled excition of the GFP linked to the neutrophil blood cells as visualized in Fig. 5 and in the zoom of one region. Fig. 5: TPEF fluorescence map excited simultanously at different wavelength emitted by the FemtoFiber dichro design biomp. Both beams share the same beam path to guarantee a spatial overlap at the sample. By tuning the respective temporal delay of both beams to zero a virtual excitation at 890 nm takes place exciting also the GFP neutrophil cell as shown in the inset. A direct comparison the retrieved SHG-map after excitation directly at 890 nm (Fig. 6) indicates the efficiency of the SHG generation of the virtual wavelength emitted by the FemtoFiber dichro design biomp and serves as reference. Page 5

6 Fig. 6: For comparison, the same section of the sample was studied using a femtosecond Ti:sapph laser at 890 nm for fluorophore excitation, showing a similar result compared to Fig. 5. To show the efficient excitation of GFP at the virtual wavelength via 2-color 2-photon excitation using the FemtoFiber dichro design biomp, the temporal delay between both laser pulses can be altered. If the pulses do not overlap, the previously obtained GFP signal (cf. Fig. 5) vanishes, as shown in Fig. 7. Fig. 7: The FemtoFiber dichro design biomp was used with both colors, 780 and 1040 nm, as previously. In contrast to Fig. 5, the temporal delay was altered, such that the laser pulses do not overlap at the sample anymore. As a consequence, the GFP neutrophil cells vanish, whereas the YFP neurons are still visible. The efficiency of the virtual excitation by calibrated reference measurements and the respective quantum yield for different materials will be evaluated in a next step. Page 6

Confocal Microscopes. Evolution of Imaging

Confocal Microscopes. Evolution of Imaging Confocal Microscopes and Evolution of Imaging Judi Reilly Hans Richter Massachusetts Institute of Technology Environment, Health & Safety Office Radiation Protection What is Confocal? Pinhole diaphragm

More information

Spectral Separation of Multifluorescence Labels with the LSM 510 META

Spectral Separation of Multifluorescence Labels with the LSM 510 META Microscopy from Carl Zeiss Spectral Separation of Multifluorescence Labels with the LSM 510 META Indians living in the South American rain forest can distinguish between almost 200 hues of green in their

More information

Genetically targeted all-optical electrophysiology with a transgenic Credependent

Genetically targeted all-optical electrophysiology with a transgenic Credependent Genetically targeted all-optical electrophysiology with a transgenic Credependent Optopatch mouse Short title: Transgenic Optopatch mouse Shan Lou 1, Yoav Adam 1, Eli N. Weinstein 1,4, Erika Williams 2,

More information

High-throughput three-dimensional (3D) lithographic microfabrication in biomedical applications

High-throughput three-dimensional (3D) lithographic microfabrication in biomedical applications High-throughput three-dimensional (3D) lithographic microfabrication in biomedical applications The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story

More information

A simple introduction to multiphoton microscopy

A simple introduction to multiphoton microscopy Journal of Microscopy, Vol. 243, Pt 3 2011, pp. 221 226 Received 29 April 2011; accepted 28 June 2011 doi: 10.1111/j.1365-2818.2011.03532.x A simple introduction to multiphoton microscopy A. USTIONE &

More information

CENTER FOR BRAIN EXPERIMENT

CENTER FOR BRAIN EXPERIMENT CENTER FOR BRAIN EXPERIMENT Section of Brain Structure Associate Professor: ARII, Tatsuo, PhD 1967 Graduated from Tohoku University, Faculty of Science. Completed the doctoral course in Engineering, Nagoya

More information

A Survey of Laser Types. Gas Lasers

A Survey of Laser Types. Gas Lasers Mihail Pivtoraiko Andrei Rozhkov Applied Optics Winter 2003 A Survey of Laser Types Laser technology is available to us since 1960 s, and since then has been quite well developed. Currently, there is a

More information

Fs- Using Ultrafast Lasers to Add New Functionality to Glass

Fs- Using Ultrafast Lasers to Add New Functionality to Glass An IMI Video Reproduction of Invited Lectures from the 17th University Glass Conference Fs- Using Ultrafast Lasers to Add New Functionality to Glass Denise M. Krol University of California, Davis 17th

More information

Bioinstrumentation Light Sources Lasers or LEDs?

Bioinstrumentation Light Sources Lasers or LEDs? Bioinstrumentation Light Sources Lasers or LEDs? A comprehensive analysis of all the factors involved in designing and building life sciences instrumentation reveals that lasers provide superior performance

More information

Lasers for Microscopy: Major Trends

Lasers for Microscopy: Major Trends Lasers for Microscopy: Major Trends Marco Arrigoni, Nigel Gallaher, Darryl McCoy, Volker Pfeufer and Matthias Schulze, Coherent Inc. Laser development for the microscopy market continues to be driven by

More information

Applicability of Hyperspectral Fluorescence Imaging to Mineral Sorting

Applicability of Hyperspectral Fluorescence Imaging to Mineral Sorting Institute of Industrial Information Technology Applicability of Hyperspectral Fluorescence Imaging to Mineral Sorting Optical Characterization of Materials, March 19, 2015 Sebastian Bauer, M.Sc. (Head:

More information

Flow Cytometry - The Essentials

Flow Cytometry - The Essentials Flow Cytometry - The Essentials Pocket Guide to Flow Cytometry: 1. Know your Cytometer 2. Understanding Fluorescence and Fluorophores 3. Gating Process 4. Controls 5. Optimization 6. Panel Building 7.

More information

AURORA AIRY BEAM LIGHT SHEET IMAGING SYSTEM THE CUSTOM DEVELOPMENT PROGRAMME

AURORA AIRY BEAM LIGHT SHEET IMAGING SYSTEM THE CUSTOM DEVELOPMENT PROGRAMME AURORA AIRY BEAM LIGHT SHEET IMAGING SYSTEM THE CUSTOM DEVELOPMENT PROGRAMME The Custom Development Programme Collaboration breeds innovation Our aim at M Squared Life, a new Biophotonics division within

More information

Imaging of protein crystals with two photon microscopy

Imaging of protein crystals with two photon microscopy Supporting Information Imaging of protein crystals with two photon microscopy Pius Padayatti,*, Grazyna Palczewska,*, Wenyu Sun, Krzysztof Palczewski,# and David Salom Polgenix Inc., Cleveland, Ohio 44106,

More information

Super-resolution Microscopy

Super-resolution Microscopy Semr oc kwhi t epaperser i es : 1. Introduction Super-resolution Microscopy Fluorescence microscopy has revolutionized the study of biological samples. Ever since the invention of fluorescence microscopy

More information

How to perform-control immunostaining experiment - microscopist subjective point of view. Pawel Pasierbek

How to perform-control immunostaining experiment - microscopist subjective point of view. Pawel Pasierbek How to perform-control immunostaining experiment - microscopist subjective point of view. Pawel Pasierbek Immunolabeling and fluorescent detection became such a standard procedure in the biomedical research

More information

Picosecond Transient Absorption Spectroscopy System. picotas

Picosecond Transient Absorption Spectroscopy System. picotas Picosecond Transient Absorption Spectroscopy System Can Easily Measure Short-Lived Intermediates. In most of light induced phenomena, intermediates (transient species) play important roles to determine

More information

Multimodal microscopy with sub-30 fs Yb fiber laser oscillator

Multimodal microscopy with sub-30 fs Yb fiber laser oscillator Multimodal microscopy with sub-30 fs Yb fiber laser oscillator Bai Nie, 1,4 Ilyas Saytashev, 1,4 Andy Chong, 2 Hui Liu, 2 Sergey N. Arkhipov, 1 Frank W. Wise, 2 and Marcos Dantus 1,3,* 1 Department of

More information

Three-Dimensional Laser Writing on the Nanometer Scale

Three-Dimensional Laser Writing on the Nanometer Scale Three-Dimensional Laser Writing on the Nanometer Scale Piezo Drives are Driving Technology Forward Page 1 of 5 The best possible positioning accuracy is now mandatory in many fields of application. The

More information

Multi-source inspection of fiber-reinforced materials

Multi-source inspection of fiber-reinforced materials 19 th World Conference on Non-Destructive Testing 2016 Multi-source inspection of fiber-reinforced materials Tomasz CHADY 1, Krzysztof GORĄCY 1 1 West Pomeranian University of Technology, Szczecin, Poland

More information

Welcome! openmicberkeley.wordpress.com. Open Berkeley

Welcome! openmicberkeley.wordpress.com. Open Berkeley Welcome! openmicberkeley.wordpress.com Agenda Jen Lee: Introduction to FRET Marla Feller: Using FRET sensors to look at time resolved measurements Becky Lamason: Using FRET to determine if a bacterial

More information

Optical Coatings. Photonics 4 Luxury Coatings , Genève. Dr. Andreas Bächli Head of Optical Coatings at RhySearch, Buchs (SG)

Optical Coatings. Photonics 4 Luxury Coatings , Genève. Dr. Andreas Bächli Head of Optical Coatings at RhySearch, Buchs (SG) Optical Coatings Photonics 4 Luxury Coatings 21.06.2017, Genève Dr. Andreas Bächli Head of Optical Coatings at RhySearch, Buchs (SG) RhySearch The Research- and Innovation Center in the Rhine Valley RhySearch

More information

Transmission Mode Photocathodes Covering the Spectral Range

Transmission Mode Photocathodes Covering the Spectral Range Transmission Mode Photocathodes Covering the Spectral Range 6/19/2002 New Developments in Photodetection 3 rd Beaune Conference June 17-21, 2002 Arlynn Smith, Keith Passmore, Roger Sillmon, Rudy Benz ITT

More information

Modification of Glass by FS Laser for Optical/Memory Applications

Modification of Glass by FS Laser for Optical/Memory Applications Modification of Glass by FS Laser for Optical/Memory Applications Kazuyuki Hirao and Kiyotaka Miura Department of Material Chemistry Kyoto University International Workshop on Scientific Challenges of

More information

Laser Welding of Engineering Plastics

Laser Welding of Engineering Plastics Laser Welding of Engineering Plastics Technical Information Further information on individual products: www.ultramid.de www.ultradur-lux.basf.com www.ultrason.de www.plasticsportal.eu/ultraform 2 LASER

More information

Methods of Culturing Microorganisms. Chapter 3. Five Basic Techniques of Culturing Bacteria. Topics

Methods of Culturing Microorganisms. Chapter 3. Five Basic Techniques of Culturing Bacteria. Topics Chapter 3 Topics Methods of Culturing Microorganisms Microscope (History, Types, Definitions) Staining (Gram s) Methods of Culturing Microorganisms Five basic techniques of culturing Media Microbial growth

More information

Live Specimen Microscopy

Live Specimen Microscopy Jens Jens Rietdorf: Rietdorf: This This presentation presentation is is meant meant give give some some general general hints hints for for live live specimen specimen microscopy microscopy Live Specimen

More information

Performance of the Micro Photon Devices PDM 50CT SPAD detector with PicoQuant TCSPC systems

Performance of the Micro Photon Devices PDM 50CT SPAD detector with PicoQuant TCSPC systems Technical Note Performance of the Micro Photon Devices PDM 5CT SPAD detector with PicoQuant TCSPC systems Rolf Krahl, Andreas Bülter, Felix Koberling, PicoQuant GmbH These measurements were performed to

More information

Boundary-breaking acoustic focusing cytometry

Boundary-breaking acoustic focusing cytometry Boundary-breaking acoustic focusing cytometry Introducing the Attune NxT Acoustic Focusing Cytometer a high-performance system that s flexible enough for any lab One of the main projects in my laboratory

More information

PULSED LASER WELDING

PULSED LASER WELDING PULSED LASER WELDING Girish P. Kelkar, Ph.D. Girish Kelkar, Ph.D, WJM Technologies, Cerritos, CA 90703, USA Laser welding is finding growing acceptance in field of manufacturing as price of lasers have

More information

REVIEW OF LASER PLASTIC WELDING PROCESS

REVIEW OF LASER PLASTIC WELDING PROCESS REVIEW OF LASER PLASTIC WELDING PROCESS Kalpesh More 1, Rushikesh Aher 2, Makrand Bharaskar 3 1,2,3 Mechanical, Sandip Institute Technology and Research Centre/Pune University, (India) ABSTRACT There are

More information

Digital resolution enhancement in surface plasmon microscopy

Digital resolution enhancement in surface plasmon microscopy Digital resolution enhancement in surface plasmon microscopy I.I. Smolyaninov 1) *, J. Elliott 2), G. Wurtz 2), A.V. Zayats 2), C.C. Davis 1) 1) Department of Electrical and Computer Engineering, University

More information

Multiphoton lithography based 3D micro/nano printing Dr Qin Hu

Multiphoton lithography based 3D micro/nano printing Dr Qin Hu Multiphoton lithography based 3D micro/nano printing Dr Qin Hu EPSRC Centre for Innovative Manufacturing in Additive Manufacturing University of Nottingham Multiphoton lithography Also known as direct

More information

Double-pulse fluorescence lifetime measurements

Double-pulse fluorescence lifetime measurements Journal of Microscopy, Vol. 186, Pt 3, June 1997, pp. 212 220. Received 15 August 1996; accepted 14 January 1997 Double-pulse fluorescence lifetime measurements A. H. BUIST,* M. MÜLLER,* E. J. GIJSBERS,*

More information

TWO-PHOTON EXCITED FLUORESCENCE OF THE LENS FOR THE DIAGNOSIS OF PRESBYOPIA

TWO-PHOTON EXCITED FLUORESCENCE OF THE LENS FOR THE DIAGNOSIS OF PRESBYOPIA ОПТИКА И СПЕКТРОСКОПИЯ, 29, том 17, 3, с. 495 499 УДК 621.373:375 НАНОФОТОНИКА И БИОМЕДИЦИНА TWO-PHOTON EXCITED FLUORESCENCE OF THE LENS FOR THE DIAGNOSIS OF PRESBYOPIA 29 г. R. Steiner, M. Kessler, O.

More information

Towards Probing Skin Cancer using Endogenous Melanin Fluorescence

Towards Probing Skin Cancer using Endogenous Melanin Fluorescence 8 Towards Probing Skin Cancer using Endogenous Melanin Fluorescence Andra Colbert, McNair Scholar, Virginia State University Faculty Research Advisor: Dr. Ahmed A. Heikal Associate Professor of Bioengineering

More information

FOBA Y-Series. High potential fiber marking lasers: More clarity, less complexity. Your product benefits

FOBA Y-Series. High potential fiber marking lasers: More clarity, less complexity. Your product benefits FOBA Y-Series High potential fiber marking lasers: More clarity, less complexity Laser marking has become one of the preferred methods for product identification, decoration and material processing. Many

More information

Size Estimation of Protein Clusters in the Nanometer Range by Using Spatially Modulated Illumination Microscopy

Size Estimation of Protein Clusters in the Nanometer Range by Using Spatially Modulated Illumination Microscopy FORMATEX 2007 A. Méndez-Vilas and J. Díaz (Eds.) Size Estimation of Protein Clusters in the Nanometer Range by Using Spatially Modulated Illumination Microscopy U. J. Birk 1,2, I. Upmann 1, D. Toomre 3,

More information

Resolution of Microscopes Visible light is nm Dry lens(0.5na), green(530nm light)=0.65µm=650nm for oil lens (1.4NA) UV light (300nm) = 0.13µm f

Resolution of Microscopes Visible light is nm Dry lens(0.5na), green(530nm light)=0.65µm=650nm for oil lens (1.4NA) UV light (300nm) = 0.13µm f Microscopes and Microscopy MCB 380 Good information sources: Alberts-Molecular Biology of the Cell http://micro.magnet.fsu.edu/primer/ http://www.microscopyu.com/ Approaches to Problems in Cell Biology

More information

Chemiluminescence Detection. Using UVP s ChemiDoc-It System

Chemiluminescence Detection. Using UVP s ChemiDoc-It System Chemiluminescence Detection Using UVP s ChemiDoc-It System Protein Detection Agents Chemiluminescence Fluorescence Colorimetric Detection Radioisotopic Detection Chemiluminescence Generation of light through

More information

Monitoring and Optimizing the Lipopolysaccharides-plasmid DNA interaction by FLIM-FRET

Monitoring and Optimizing the Lipopolysaccharides-plasmid DNA interaction by FLIM-FRET Transactions on Science and Technology Vol. 4, No. 3-3, 342-347, 2017 Monitoring and Optimizing the Lipopolysaccharides-plasmid DNA interaction by FLIM-FRET Nur Syahadatain Abdul Razak 1#, Clarence M.

More information

FLOW CYTOMETRY. CyAn ADP. Analyzer

FLOW CYTOMETRY. CyAn ADP. Analyzer FLOW CYTOMETRY CyAn ADP Analyzer Experience the Power of the CyAn ADP and its optimal performance The Power of Detection The Power of Speed The Power of Ease The CyAn ADP Analyzer is the next step in Advanced

More information

Multiphoton Redox Ratio Imaging for Metabolic Monitoring In Vivo

Multiphoton Redox Ratio Imaging for Metabolic Monitoring In Vivo Chapter 11 Multiphoton Redox Ratio Imaging for Metabolic Monitoring In Vivo Melissa Skala and Nirmala Ramanujam Summary Metabolic monitoring at the cellular level in live tissues is important for understanding

More information

Partha Roy

Partha Roy Fluorescence microscopy http://micro.magnet.fsu.edu/primer/index.html Partha Roy 1 Lecture Outline Definition of fluorescence Common fluorescent reagents Construction ti of a fluorescence microscope Optical

More information

Standard Optics Information

Standard Optics Information INFRASIL 301, 302 1. GENERAL PRODUCT DESCRIPTION Heraeus INFRASIL 301 and 302 are optical quartz glass grades manufactured by fusion of natural quartz crystals in an electrically heated furnace. They combine

More information

Greenhouse Gas Measurement Technology

Greenhouse Gas Measurement Technology Greenhouse Gas Measurement Technology Michael Corvese Thermo Fisher Scientific March 25, 2010 Agenda Introduction GHG Regulatory Current Methods Infrared Spectroscopy GC FID Emerging Methods Laser-Based

More information

Flow Cytometry. Flow Cytometry Basics Guide

Flow Cytometry. Flow Cytometry Basics Guide Flow Cytometry Flow Cytometry Basics Guide Table of Contents Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Principles of the Flow Cytometer Fluidics System.... 3 Optics and Detection.... 4 Signal and

More information

Next Level of Super Resolution Fluorescence Microscopy

Next Level of Super Resolution Fluorescence Microscopy Work in your familiar GFP/YFP/RFP system from the first experiment to the nanoimage Bwcon business award winner: Inventor Prof Christoph Cremer Next Level of Super Resolution Fluorescence Microscopy Resolution:

More information

Innovations To Meet Your Needs

Innovations To Meet Your Needs Innovations To Meet Your Needs Cooled CCD Camera 1340 x 1037 pixel resolution for greatest image quality 12-bit precision provides 3 orders of linear dynamic range Windows and Power Macintosh Software

More information

3D Laser Lithography in Biotechnology and Medical Technology

3D Laser Lithography in Biotechnology and Medical Technology 3D Laser Lithography in Biotechnology and Medical Technology High-Precision, Piezo-Based Nanopositioning Systems Advance Technology Page 1 of 6 Laser technology makes it possible to create even very complex

More information

Experimental study on laser marking of alumina

Experimental study on laser marking of alumina Lasers in Manufacturing Conference 2015 Experimental study on laser marking of alumina J. Penide a*, F. Quintero a, F. Arias-González a, A. Fernández b, J. del Val a, R. Comesaña c, A. Riveiro a, F. Lusquiños

More information

Introduction to Fluorescence Jablonski Diagram

Introduction to Fluorescence Jablonski Diagram ntroduction to Fluorescence Jablonski Diagram Excited Singlet Manifold S1 internal conversion S2 k -isc k isc Excited riplet Manifold 1 S0 k nr k k' f nr fluorescence k p phosphorescence Singlet round

More information

LOW TEMPERATURE PHOTONIC SINTERING FOR PRINTED ELECTRONICS. Dr. Saad Ahmed XENON Corporation November 19, 2015

LOW TEMPERATURE PHOTONIC SINTERING FOR PRINTED ELECTRONICS. Dr. Saad Ahmed XENON Corporation November 19, 2015 LOW TEMPERATURE PHOTONIC SINTERING FOR PRINTED ELECTRONICS Dr. Saad Ahmed XENON Corporation November 19, 2015 Topics Introduction to Pulsed Light Photonic sintering for Printed Electronics R&D Tools for

More information

Characterization of laser-material interaction during laser cladding process P.-A. Vetter,* J. Fontaine,* T. Engel," L. Lagrange,& T.

Characterization of laser-material interaction during laser cladding process P.-A. Vetter,* J. Fontaine,* T. Engel, L. Lagrange,& T. Characterization of laser-material interaction during laser cladding process P.-A. Vetter,* J. Fontaine,* T. Engel," L. Lagrange,& T. Marchione^ f^, BID de /a rzcfozre ^7000 France ABSTRACT The interaction

More information

A LIDAR SYSTEM FOR REMOTE MEASUREMENT OF OIL FILM THICKNESS ON SEA SURFACE *

A LIDAR SYSTEM FOR REMOTE MEASUREMENT OF OIL FILM THICKNESS ON SEA SURFACE * A LIDAR SYSTEM FOR REMOTE MEASUREMENT OF OIL FILM THICKNESS ON SEA SURFACE * Jacek Piskozub, Violetta Drozdowska Institute of Oceanology PAS, Sopot, Poland; email: piskozub@iopan.gda Viktor Varlamov Institute

More information

Laser Polymer Welding - No Limits for Colors

Laser Polymer Welding - No Limits for Colors Press Release 'K 2004' BASF, TREFFERT and ROFIN present new laser additives for welding polymers in any color combination: Laser Polymer Welding - No Limits for Colors LUDWIGSHAFEN / BINGEN / STARNBERG

More information

Introduction to N-STORM

Introduction to N-STORM Introduction to N-STORM Dan Metcalf Advanced Imaging Manager Outline Introduction Principles of STORM Applications N-STORM overview Biological Scale Mitochondrion Microtubule Amino Acid 1Å Kinesin 1nm

More information

Time-resolved Measurements Using the Agilent Cary Eclipse Fluorescence Spectrophotometer A Versatile Instrument for Accurate Measurements

Time-resolved Measurements Using the Agilent Cary Eclipse Fluorescence Spectrophotometer A Versatile Instrument for Accurate Measurements Time-resolved Measurements Using the Agilent Cary Eclipse Fluorescence Spectrophotometer A Versatile Instrument for Accurate Measurements Technical Overview Authors Dr. Fabian Zieschang, Katherine MacNamara,

More information

QImaging Camera Application Notes Multicolor Immunofluorescence Imaging

QImaging Camera Application Notes Multicolor Immunofluorescence Imaging QImaging Camera Application Notes Multicolor Immunofluorescence Imaging In order to image localization of intracellular proteins with high specificity, it is frequently necessary to multiplex antibody

More information

Quantum Dot applications in Fluorescence Imaging for Calibration and Molecular Imaging

Quantum Dot applications in Fluorescence Imaging for Calibration and Molecular Imaging Quantum Dot applications in Fluorescence Imaging for Calibration and Molecular Imaging Introduction In this application note, we will discuss the application of quantum dots in fluorescence imaging, both

More information

Practical light microscopy: an introduction

Practical light microscopy: an introduction Practical light microscopy: an introduction Dr. Mark Leake, Oxford University www.physics.ox.ac.uk/users/leake Aim of today s talk: Explanation of the very (very) basics of how a light microscope works

More information

RARE-EARTH DOPED PHOSPHATE GLASSES FOR NEODYMIUM LASER SYSTEMS POSSESSING A GREATLY ENHANCED PUMP POWER CONVERSION

RARE-EARTH DOPED PHOSPHATE GLASSES FOR NEODYMIUM LASER SYSTEMS POSSESSING A GREATLY ENHANCED PUMP POWER CONVERSION Submit To: The American Ceramic Society, 12 Annual Meeting & Exposition April 3 May 3, 2, St. Louis, Missouri RARE-EARTH DOPED PHOSPHATE GLASSES FOR NEODYMIUM LASER SYSTEMS POSSESSING A GREATLY ENHANCED

More information

In-Situ Characterization During MOVPE Growth of III-Nitrides using Reflectrometry

In-Situ Characterization During MOVPE Growth of III-Nitrides using Reflectrometry 18 Annual Report 1999, Dept. of Optoelectronics, University of Ulm In-Situ Characterization During MOVPE Growth of III-Nitrides using Reflectrometry Christoph Kirchner and Matthias Seyboth The suitability

More information

BBO Crystals. Features. Broad phase-matchable second-harmonic-generation (SHG) range from nm to 2500 nm

BBO Crystals. Features. Broad phase-matchable second-harmonic-generation (SHG) range from nm to 2500 nm BBO Crystals Broad phase-matchable second-harmonic-generation (SHG) range from 409.6 nm to 2500 nm Wide transparency range from 189 nm to 2600 nm High damage threshold of 10 J/cm 2 for 10 ns pulse-width

More information

Application of Quantum Mechanics to Biology

Application of Quantum Mechanics to Biology Application of Quantum Mechanics to Biology How can we apply quantum mechanics to biology? Polymers of nucleotides and amino acids - millions of atoms bounded into a large molecule Visual System Must turn

More information

MICROFABRICATION OF OPTICALLY ACTIVE InO X MICROSTRUCTURES BY ULTRASHORT LASER PULSES

MICROFABRICATION OF OPTICALLY ACTIVE InO X MICROSTRUCTURES BY ULTRASHORT LASER PULSES Journal of Optoelectronics and Advanced Materials Vol. 4, No. 3, September 2002, p. 809-812 MICROFABRICATION OF OPTICALLY ACTIVE InO X MICROSTRUCTURES BY ULTRASHORT LASER PULSES Foundation for Research

More information

Chapter 1 Nanophotonics and Single Molecules

Chapter 1 Nanophotonics and Single Molecules Chapter 1 Nanophotonics and Single Molecules W.E. Moerner(*ü ), P. James Schuck, David P. Fromm, Anika Kinkhabwala, Samuel J. Lord, Stefanie Y. Nishimura, Katherine A. Willets, Arvind Sundaramurthy, Gordon

More information

Recent Research Projects:

Recent Research Projects: Recent Research Projects: 1. Musical Tuning Enhanced In-Vitro Micro/Nano Palpation of Cells: A Multi- Modal and Interactive System Engineering Approach for Cellular Pathology Studies Project Summary: In-vitro

More information

Challenges to measuring intracellular Ca 2+ Calmodulin: nature s Ca 2+ sensor

Challenges to measuring intracellular Ca 2+ Calmodulin: nature s Ca 2+ sensor Calcium Signals in Biological Systems Lecture 3 (2/9/0) Measuring intracellular Ca 2+ signals II: Genetically encoded Ca 2+ sensors Henry M. Colecraft, Ph.D. Challenges to measuring intracellular Ca 2+

More information

SAPIENZA Università di Roma Laurea magistrale in Ingegneria delle Nanotecnologie A.A Biophotonics Laboratory Course

SAPIENZA Università di Roma Laurea magistrale in Ingegneria delle Nanotecnologie A.A Biophotonics Laboratory Course SAPIENZA Università di Roma Laurea magistrale in Ingegneria delle Nanotecnologie A.A. 2016-2017 Biophotonics Laboratory Course Prof. Francesco Michelotti SAPIENZA Università di Roma Facoltà di Ingegneria

More information

Imagerie et spectroscopie de fluorescence par excitation non radiative

Imagerie et spectroscopie de fluorescence par excitation non radiative Imagerie et spectroscopie de fluorescence par excitation non radiative comment s affranchir de la limite de diffraction Rodolphe Jaffiol, Cyrille Vézy, Marcelina Cardoso Dos Santos LNIO, UTT, Troyes NanoBioPhotonics

More information

First Electrically Pumped Hybrid Silicon Laser

First Electrically Pumped Hybrid Silicon Laser First Electrically Pumped Hybrid Silicon Laser UCSB Engineering Insights Oct 18 th 2006 Mario Paniccia Intel Corporation 1 Sept 18 th 2006 What We are Announcing Research Breakthrough: 1st Electrically

More information

Fpollutant-free zero air for analytical

Fpollutant-free zero air for analytical Ambient Air Quality Monitoring System AQMS-100 Zero Air Generator PI AQMS-100 provides ultra pure Fpollutant-free zero air for analytical purpose. Remote monitoring and configuration can also be achieved

More information

Photoluminescence Spectroscopy on Chemically Synthesized Nanoparticles

Photoluminescence Spectroscopy on Chemically Synthesized Nanoparticles Photoluminescence Spectroscopy on Chemically Synthesized Nanoparticles Torben Menke 1 Institut für Angewandte Physik und Zentrum für Mikrostrukturforschung Universität Hamburg Diploma Talk, 18.12.27 1

More information

NovoCyte Flow Cytometer

NovoCyte Flow Cytometer NovoCyte Flow Cytometer The Flow Cytometer for Everyone 2 Experience the NovoCyte Advantage Focus on advancing your research. Let the flow cytometer do the rest. NovoCyte Flow Cytometer High Performance

More information

User guide and application notes for Zeiss LSM7 Multiphoton intravital microscope

User guide and application notes for Zeiss LSM7 Multiphoton intravital microscope User guide and application notes for Zeiss LSM7 Multiphoton intravital microscope Biomedicum Imaging Unit (BIU) University of Helsinki Written by Dmitry Molotkov, BIU Edited by Marja Lohela, BIU September

More information

Lighting research Toulouse team (France) Ludovic VANQUIN Ikbal MARGHAD Lydie AREXIS BOISSON

Lighting research Toulouse team (France) Ludovic VANQUIN Ikbal MARGHAD Lydie AREXIS BOISSON 1 Lighting research Toulouse team (France) Ludovic VANQUIN Ikbal MARGHAD Lydie AREXIS BOISSON Plan 2 INTRODUCTION I. Medical Imaging for the diagnosis of the Alzheimer s disease (Ludovic) II.Lighting display:

More information

Photonic Drying Pulsed Light as a low Temperature Sintering Process

Photonic Drying Pulsed Light as a low Temperature Sintering Process Photonic Drying Pulsed Light as a low Temperature Sintering Process Lou Panico Xenon Corporation W E S T E R N M I C H I G A N U N I V E R S I T Y PRESENTATION OVERVIEW What is Printed Electronics Materials

More information

Advances in Intense Pulsed Light Solutions For Display Manufacturing. XENON Corporation Dr. Saad Ahmed Japan IDW 2016

Advances in Intense Pulsed Light Solutions For Display Manufacturing. XENON Corporation Dr. Saad Ahmed Japan IDW 2016 Advances in Intense Pulsed Light Solutions For Display Manufacturing XENON Corporation Dr. Saad Ahmed Japan IDW 2016 Talk Outline Introduction to Pulsed Light Applications in Display UV Curing Applications

More information

Measurement of surface concentration of fluorophores using. fluorescence fluctuation spectroscopy

Measurement of surface concentration of fluorophores using. fluorescence fluctuation spectroscopy Measurement of surface concentration of fluorophores using fluorescence fluctuation spectroscopy A. Delon 1, J. Derouard 1, G. Delapierre and R. Jaffiol 1 (1) Laboratoire de Spectrométrie Physique (UMR

More information

The neov endovascular

The neov endovascular www.neo-laser.com The neov endovascular Surgical power was never so small The neov1470 surgical laser the smallest, lightest weight, and most portable laser system of its power class. DESIGNED FOR SURGERY

More information

Laser Dicing of Silicon: Comparison of Ablation Mechanisms with a Novel Technology of Thermally Induced Stress

Laser Dicing of Silicon: Comparison of Ablation Mechanisms with a Novel Technology of Thermally Induced Stress Dicing of Silicon: Comparison of Ablation Mechanisms with a Novel Technology of Thermally Induced Stress Oliver HAUPT, Frank SIEGEL, Aart SCHOONDERBEEK, Lars RICHTER, Rainer KLING, Andreas OSTENDORF Zentrum

More information

Basics of Plasmonics

Basics of Plasmonics Basics of Plasmonics Min Qiu Laboratory of Photonics and Microwave Engineering School of Information and Communication Technology Royal Institute of Technology (KTH) Electrum 229, 16440 Kista, Sweden http://www.nanophotonics.se/

More information

Microstructuring of Steel and Hard Metal using Femtosecond Laser Pulses

Microstructuring of Steel and Hard Metal using Femtosecond Laser Pulses Available online at www.sciencedirect.com Physics Procedia 12 (2011) 60 66 LiM 2011 Microstructuring of Steel and Hard Metal using Femtosecond Laser Pulses Manuel Pfeiffer a *, Andy Engel a, Steffen Weißmantel

More information

MAKE NO MISTAKE, IT S UV THAT EVERYONE CAN USE. LAMBDA 265 / 365 / 465 UV/Vis Solutions

MAKE NO MISTAKE, IT S UV THAT EVERYONE CAN USE. LAMBDA 265 / 365 / 465 UV/Vis Solutions LAMBDA 265 / 365 / 465 UV/Vis Solutions MAKE NO MISTAKE, IT S UV THAT EVERYONE CAN USE 2 PUTTING OUR FOCUS ON THE NEEDS OF INDUSTRY AND ACADEMIA Based on our long history of UV/Vis leadership, our LAMBDA

More information

GROWTH, OPTICAL AND STRUCTURAL CHARACTERIZATION OF SEMI ORGANIC NON LINEAR OPTICAL THIOSEMICARBAZIDE BORATE (TSCB) SINGLE CRYSTALS

GROWTH, OPTICAL AND STRUCTURAL CHARACTERIZATION OF SEMI ORGANIC NON LINEAR OPTICAL THIOSEMICARBAZIDE BORATE (TSCB) SINGLE CRYSTALS Indian Journal of Pure and Applied Physics (IJPAP) Vol.1.No.1 2013 pp 7-11 available at: www.goniv.com Paper Received :10-04-2013 Paper Published:26-04-2013 Paper Reviewed by: 1. Dr.S.Selvakumar 2. Hendry

More information

SIL-based confocal fluorescence microscope for investigating individual nanostructures

SIL-based confocal fluorescence microscope for investigating individual nanostructures Cent. Eur. J. Phys. 9(2) 2011 293-299 DOI: 10.2478/s11534-010-0098-5 Central European Journal of Physics SIL-based confocal fluorescence microscope for investigating individual nanostructures Research

More information

Search and Characterization of Optical Ceramics and Crystals for Diode-pumped Laser Oscillations

Search and Characterization of Optical Ceramics and Crystals for Diode-pumped Laser Oscillations AFRL-AFOSR-UK-TR-2013-0063 Search and Characterization of Optical Ceramics and Crystals for Diode-pumped Laser Oscillations Professor Tasoltan Basiev Russian Academy of Sciences Laser Materials and Technologies

More information

CREOL, The College of Optics & Photonics, University of Central Florida

CREOL, The College of Optics & Photonics, University of Central Florida Metal Substrate Induced Control of Ag Nanoparticle Plasmon Resonances for Tunable SERS Substrates Pieter G. Kik 1, Amitabh Ghoshal 1, Manuel Marquez 2 and Min Hu 1 1 CREOL, The College of Optics and Photonics,

More information

IWAN W. SCHIE, CENTER FOR BIOPHOTONICS, SCIENCE, AND TECHNOLOGY, UNIVERSITY OF CALIFORNIA DAVIS, SACRAMENTO, CA 95817, USA

IWAN W. SCHIE, CENTER FOR BIOPHOTONICS, SCIENCE, AND TECHNOLOGY, UNIVERSITY OF CALIFORNIA DAVIS, SACRAMENTO, CA 95817, USA IWAN W. SCHIE, CENTER FOR BIOPHOTONICS, SCIENCE, AND TECHNOLOGY, UNIVERSITY OF CALIFORNIA DAVIS, SACRAMENTO, CA 95817, USA THOMAS HUSER DEPARTMENT OF PHYSICS AND CENTER FOR BIOPHOTONICS, UNIVERSITY OF

More information

Cavity Filters. KIGRE, INC., 100 Marshland Road, Hilton Head, SC 29926, USA PH: FAX: Web:

Cavity Filters. KIGRE, INC., 100 Marshland Road, Hilton Head, SC 29926, USA PH: FAX: Web: Cavity Filters Kigre, Inc. s expertise in laser glass technology has led the way in the company s development of a wide range of cavity filter glass for solid-state lasers. Filters are used inside the

More information

arxiv: v3 [physics.med-ph] 10 Aug 2015

arxiv: v3 [physics.med-ph] 10 Aug 2015 3D super-resolved in vitro multiphoton microscopy by saturation of excitation arxiv:1507.01408v3 [physics.med-ph] 10 Aug 2015 Anh Dung Nguyen, 1, Franois Duport, 1 Arno Bouwens, 1 Frédérique Vanholsbeeck,

More information

Supporting Information to Carbon Nanodots Towards a Comprehensive Understanding of their Photoluminescence

Supporting Information to Carbon Nanodots Towards a Comprehensive Understanding of their Photoluminescence Supporting Information to Carbon Nanodots Towards a Comprehensive Understanding of their Photoluminescence Volker Strauss, a, Johannes T. Margraf, a,b, Christian Dolle, c Benjamin Butz, c Thomas J. Nacken,

More information

Delivering top performance. Outstanding value. Diagnostic scalability. ACUSON X150 Ultrasound System.

Delivering top performance. Outstanding value. Diagnostic scalability. ACUSON X150 Ultrasound System. Delivering top performance. Outstanding value. Diagnostic scalability. ACUSON X150 Ultrasound System www.siemens.com/medical Visualize more. Perform more effectively. Diagnose more confidently. Designed

More information

Characterization of Materials with a Combined AFM/Raman Microscope

Characterization of Materials with a Combined AFM/Raman Microscope Characterization of Materials with a Combined AFM/Raman Microscope Marko Surtchev 1, Sergei Magonov 1 and Mark Wall 2 1 NT-MDT America, Tempe, AZ U.S.A. 2 Thermo Fisher Scientific, Madison, WI, U.S.A.

More information

Solar Cells and Photosensors.

Solar Cells and Photosensors. Designing Photonic Crystals in Strongly Absorbing Material for Applications in Solar Cells and Photosensors. Minda Wagenmaker 1, Ebuka S. Arinze 2, Botong Qiu 2, Susanna M. Thon 2 1 Mechanical Engineering

More information

Fluorescent Sensor Detects Microbes in Real Time

Fluorescent Sensor Detects Microbes in Real Time Fluorescent Sensor Detects Microbes in Real Time BioVigilant has developed an optical technology that can determine the quantity and size of particles in liquid or air, and simultaneously determine whether

More information

Azure Biosystems Western Blotting Workflow

Azure Biosystems Western Blotting Workflow Azure Biosystems Western Blotting Workflow PROBE PLAN SEPARATE ANALYZE VISUALIZE PLAN Plan your experiment and choose your detection method Chemiluminescent Western Blotting The most common method for

More information

Extended Time-Lapse Multimodal Microscopy for Tracking Cell and Tissue Dynamics in Skin

Extended Time-Lapse Multimodal Microscopy for Tracking Cell and Tissue Dynamics in Skin Extended Time-Lapse Multimodal Microscopy for Tracking Cell and Tissue Dynamics in Skin Stephen A. Boppart, M.D., Ph.D. Biophotonics Imaging Laboratory Beckman Institute for Advanced Science and Technology

More information