Classical (Mendelian) Genetics. Gregor Mendel
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1 Classical (Mendelian) Genetics Gregor Mendel
2 Vocabulary Genetics: The scientific study of heredity Allele: Alternate forms of a gene/factor. Genotype: combination of alleles an organism has. Phenotype: How an organism appears. Dominant: An allele which is expressed (masks the other). Recessive: An allele which is present but remains unexpressed (masked) Homozygous: Both alleles for a trait are the same. Heterozygous: The organism's alleles for a trait are different.
3 History Principles of genetics were developed in the mid 19th century by Gregor Mendel an Austrian Monk Developed these principles without ANY scientific equipment - only his mind. Experimented with pea plants, by crossing various strains and observing the characteristics of their offspring. Studied the following characteristics: Pea color (Green, yellow) Pea shape (round, wrinkled) Flower color (purple, white) Plant height (tall, short) Made the following observations (example given is pea shape) When he crossed a round pea and wrinkled pea, the offspring (F1 gen.) always had round peas. When he crossed these F1 plants, however, he would get offspring which produced round and wrinkled peas in a 3:1 ratio.
4 Laws of Inheretance Law of Segregation: When gametes (sperm egg etc ) are formed each gamete will receive one allele or the other. Law of independent assortment: Two or more alleles will separate independently of each other when gametes are formed
5 Punnett Squares Genetic problems can be easily solved using a tool called a punnett square. Tool for calculating genetic probabilities A punnett square
6 Monohybrid cross (cross with only 1 trait) Problem: Using this is a several step process, look at the following example Tallness (T) is dominant over shortness (t) in pea plants. A Homozygous tall plant (TT) is crossed with a short plant (tt). What is the genotypic makeup of the offspring? The phenotypic makeup?
7 1. Determine alleles of each parent, these are given as TT, and tt respectively. 2. Take each possible allele of each parent, separate them, and place each allele either along the top, or along the side of the punnett square. Punnet process
8 Punnett process continued Lastly, write the letter for each allele across each column or down each row. The resultant mix is the genotype for the offspring. In this case, each offspring has a Tt (heterozygous tall) genotype, and simply a "Tall" phenotype.
9 Punnett process continued Lets take this a step further and cross these F1 offspring (Tt) to see what genotypes and phenotypes we get. Since each parent can contribute a T and a t to the offspring, the punnett square should look like this.
10 Punnett process continued Here we have some more interesting results: First we now have 3 genotypes (TT, Tt, & tt) in a 1:2:1 genotypic ratio. We now have 2 different phenotypes (Tall & short) in a 3:1 Phenotypic ratio. This is the common outcome from such crosses.
11 Dihybrid crosses Dihybrid crosses are made when phenotypes and genotypes composed of 2 independent alleles are analyzed. Process is very similar to monohybrid crosses. Example: 2 traits are being analyzed Plant height (Tt) with tall being dominant to short, Flower color (Ww) with Purple flowers being dominant to white.
12 Dihybrid cross example The cross with a pure-breeding (homozygous) Tall,Purple plant with a pure-breeding Short, white plant should look like this. F1 generation
13 Dihybrid cross example continued Take the offspring and cross them since they are donating alleles for 2 traits, each parent in the f1 generation can give 4 possible combination of alleles. TW, Tw, tw, or tw. The cross should look like this. (The mathematical foil method can often be used here) F2 Generation
14 Dihybrid cross example continued Note that there is a 9:3:3:1 phenotypic ratio. 9/16 showing both dominant traits, 3/16 & 3/16 showing one of the recessive traits, and 1/16 showing both recessive traits. Also note that this also indicates that these alleles are separating independently of each other. This is evidence of Mendel's Law of independent assortment
15 Chromosomes and Classical Genetics Walter Sutton in 1902 proposed that chromosomes were the physical carriers of Mendel's alleles Problems arose however regarding the following question: Why are the number of alleles which undergo independent assortment greater than the number of chromosomes of an organism? This was explained understanding of 2 additional factors; Sex Linkage and crossing over
16 Sex Linkage All chromosomes are homologous except on sex chromosomes. Sex chromosomes are either X or Y. If an organism is XX, it is a female, if XY it is male. If a recessive allele exists on the X chromosome. It will not have a corresponding allele on the Y chromosome, and will therefore always be expressed
17 Sex linkage example Recessive gene for white eye color located on the X w chromosome of Drosophila. All Males which receive this gene during fertilization (50%) will express this. If a female receives the X w chromosome. It will usually not be expressed since she carries an X chromosome with the normal gene
18 Human Sex Linkage Hemophilia: Disorder of the blood where clotting does not occur properly due to a faulty protein. Occurs on the X chromosome, and is recessive. Thus a vast majority of those affected are males. First known person known to carry the disorder was Queen Victoria of England. Thus all those affected are related to European royalty.
19 Hemophilia and Royalty
20 Other Factors: Multiple Alleles Phenotypes are controlled by more than 1 allele. Eg. Blood types are regulated by 3 separate genes. ABO Blood typing Humans have multiple types of surface antigens on RBC's The nature of these surface proteins determines a person's Blood Type. There are 3 alleles which determine blood type I A, I B, or I O. This is referred to as having multiple alleles Human blood types are designated as A, B or O. Type A denotes having the A surface antigen, and is denoted by I A Type B denotes having the B surface antigen, and is denoted by I B Type O denotes having neither A or B surface antigen, and is denoted by I O There are several blood type combinations possible A B AB (Universal recipient) O (Universal donor)
21 Blood & Immunity A person can receive blood only when the donor's blood type does not contain any surface antigen the recipient does not. This is because the recipient has antibodies which will attack any foreign surface protein. Thus, Type AB can accept any blood types because it will not attack A or B surface antigens. However, a type AB person could only donate blood to another AB person. They are known as Universal Recipients. Also, Type O persons are Universal donors because they have NO surface antigens that recipients' immune systems can attack. Type O persons can ONLY receive blood from other type O persons. There is another blood type factor known as Rh. People are either Rh+ or Rh- based on a basic dominant/recessive mechanism. Not usually a problem except with pregnancy. It is possible that an Rh- mother can carry an Rh+ fetus and develop antibodies which will attack & destroy the fetal blood This usually occurs with 2nd or 3rd pregnancies, and is detectable and treatable.
22 Other Factors: Incomplete Dominance Some alleles for a gene are not completely dominant over the others. This results in partially masked phenotypes which are intermediate to the two extremes.
23 Other Factors: Continuous Variation Many traits may have a wide range of continuous values. Eg. Human height can vary considerably. There are not just "tall" or "short" humans
24 Other Factors Gene interaction: Many biological pathways are governed by multiple enzymes, involving multiple steps. If any one of these steps are altered. The end product of the pathway may be disrupted. Environmental effects: Sometimes genes will not be fully expressed owing to external factors. Example: Human height may not be fully expressed if individuals experience poor nutrition.
25 The Average American Phenotype
26 The Average Northern Guilford High School Phenotype
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