Sanquin Blood Grouping Reagents

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1 Sanquin Blood Grouping Reagents Blood grouping reagents are essential tools for diagnostic laboratories specialised in serology. The broad range of reagents developed by Sanquin Reagents is used in several serological tests, especially focused on red blood cells and platelets. These diagnostic tests are of the highest importance before a blood transfusion (with donor red blood cells or platelets) is given, or to prevent immunisation during pregnancy. Highlights of Sanquin blood grouping reagents are: Cellbind is Sanquin s innovative technique. In this affinity column technique antibodies bound to red blood cells will, after centrifugation, be captured at the top of the gel in the microcolumns. One card for all tests! Magister: fully automated immunohaematology analyser in combination with Cellbind. PeliPIP is a software program for the identification of irregular antibodies. Column panels for use in column agglutination techniques. A broad range of monoclonal reagents for typing rare blood groups. Pelicontrol: a whole blood control for use in all manual and automated typing and screening techniques. External Quality Control program in combination with PeliCase 1. MASPAT: Cross-matching assay for the detection of platelet antibodies in serum. Reagents for typing blood group antigens on erythrocytes AB0 blood grouping reagents order description 3x10ml K11883 Pelikloon anti-a (IgM) Saline; slide/spin tube/mtp 10x10ml K Pelikloon anti-a (IgM) Saline; slide/spin tube/mtp 3x10ml K11893 Pelikloon anti-b (IgM) Saline; slide/spin tube/mtp 10x10ml K Pelikloon anti-b (IgM) Saline; slide/spin tube/mtp 3x10ml K11903 Pelikloon anti-a,b (IgM) Saline; slide/spin tube/mtp 10x10ml K Pelikloon anti-a,b (IgM) Saline; slide/spin tube/mtp Lectines order description 5ml K1328 anti-a1 (Dolichos biflorus) Saline; slide/spin tube 3ml K1327 MTP = Microtiter plate anti-h (Ulex europaeus) Saline; spin tube

2 Rhesus blood grouping reagents 10ml K1155 Pelikloon anti-d (IgM) Saline; slide/spin tube/mtp 10x10ml K Pelikloon anti-d (IgM) Saline; slide/spin tube/mtp 10ml K1153 Pelikloon anti-d enhanced (IgG/IgM/albumin) one phase reagent for direct detection of (weak) D- positive erythrocytes and D-variants Saline; spin tube/mtp 10ml K1157 Pelikloon anti-d Mix (IgG/IgM) two phase reagent: IgM: direct agglutination (weak) D-positive erythrocytes IgG: agglutination weak D and D variants in the IAT Saline/IAT; slide/spin tube/mtp 10x10ml K Pelikloon anti-d Mix (IgG/IgM) Saline/IAT; slide/spin tube/mtp 5ml K1195 Pelikloon anti-c (IgM) Saline; slide/spin tube/mtp 5ml K1196 Pelikloon anti-c (IgM) Saline; slide/spin tube/mtp 5ml K1191 Pelikloon anti-e (IgM) Saline; slide/spin tube/mtp 5ml K1197 Pelikloon anti-e (IgM) Saline; slide/spin tube/mtp 2ml K1201 Pelikloon anti-c w (IgM) Saline; spin tube 10 ml K1113 Pelikloon anti- CDE (IgM/IgG) Saline; slide/spin tube/mtp Second line rhesus phenotyping reagents (for clone s see website) 5ml K1202 Pelikloon anti-c (IgM) Saline; slide/spin tube/mtp 5ml K1203 Pelikloon anti-c (IgM) Saline; slide/spin tube/mtp 5ml K1204 Pelikloon anti-e (IgM) Saline; slide/spin tube/mtp 5ml K1205 Pelikloon anti-e (IgM) Saline; slide/spin tube/mtp MTP = Microtiter plate; Rare blood grouping reagents 5ml K1199 Pelikloon anti-k (IgM) Saline; slide/spin tube/mtp 2ml K1300 Pelikloon anti-jk a (IgM) Saline; spin tube 2ml K1301 Pelikloon anti-jk b (IgM) Saline; spin tube 2ml K1302 Pelikloon anti-m (IgG) Saline; spin tube 2ml K1303 Pelikloon anti-n (IgG) Saline; spin tube 2ml K1304 Pelikloon anti-s (IgM) Saline; spin tube 2ml K1305 Pelikloon anti-p1 (IgM) Saline; spin tube 2ml K1306 Pelikloon anti-le a (IgM) Saline; spin tube 2ml K1307 Pelikloon anti-le b (IgM) Saline; spin tube 2ml K1310 Pelikloon anti-k (IgM) Saline; spin tube Polyclonal 3ml K1334 anti-kp a (IgG) IAT; spin tube 3ml K1335 anti-kp b (IgG) IAT; spin tube 3ml K1336 anti-fy a (IgG) IAT; spin tube 3ml K1337 anti-fy b (IgG) IAT; spin tube 3ml K1340 anti-lu a (IgG) IAT; spin tube 3ml K1341 anti-lu b (IgG) IAT; spin tube

3 3ml K1343 anti-s (IgG) IAT; spin tube 3ml K1344 anti-wr a (IgG) IAT; spin tube MTP = Microtiter plate; Control reagents for blood typing Control monoclonal reagents 10 ml K1156 Pelikloon control Control on false-positive results, obtained with monoclonal blood grouping reagents 10 ml K1152 Pelikloon enhanced control Control on false-positive results, obtained with anti-d enhanced reagent Control polyclonal reagents 10ml K1146 pooled AB serum Control on false-positive results, obtained with polyclonal blood grouping reagents Reagents for the indirect antiglobulin test (Coombs sera) Polyspecific order description 10ml K1193 polyspecific anti-human serum polyclonal anti-igg & monoclonal anti-c3d 10ml K1194 polyspecific anti-human serum (green solution) polyclonal anti-igg & monoclonal anti-c3d Monospecific (LISS or albumin)-iat/dat; spin tube (LISS or albumin)-iat/dat; spin tube 10ml K1131 monospecific anti-human IgG (LISS or PEG)-IAT/DAT; spin tube 10ml K1124 monospecific anti-human IgG (green solution) (LISS or PEG)-IAT/DAT; spin tube 5ml K1377 Pelikloon anti-human C3d DAT; spin tube 5ml K1378 Pelikloon anti-human C3c and -C3d DAT; spin tube 5ml K1375 monospecific anti-human IgA DAT; spin tube 5ml K1376 monospecific anti-human IgM DAT; spin tube Control 10ml K1192 Coombs control reagent serum with a weak anti-d activity to control the performance of the IAT ; DAT=Direct antiglobulin test Albumin/(LISS or PEG)-IAT; spin tube Agglutination potentiating reagents BSA (Bovine Serum Albumin) 10ml K1106 BSA 22% BSA-IAT; spin tube 10ml K1107 BSA 30% BSA-IAT; spin tube PEG (Polyethylene glycol) 20ml K1159 PEG % solution PEG-IAT; spin tube

4 10x20ml K PEG % solution PEG-IAT; spin tube LISS (modified Low Ionic Strength Solution) 10ml K1110 PeliLISS modified low ionic strength solution, containing high molecular additives LISS-IAT; spin tube Enzyme order description 5ml K1121 Bromelin (lyophilized) enzyme-test; spin tube Reagent red blood cells (3% cell suspension) Red blood cells for confirmation of AB0 groups Confirmation of AB0 groups (AB0 reverse group) order description 10ml K1140 erythrocytes A1 Saline; slide/spin tube 10ml K1142 erythrocytes B Saline; slide/spin tube Control cells order description 10ml K1141 erythrocytes A2 Saline; slide/spin tube 10ml K1143 erythrocytes 0, D-positive Saline; slide/spin tube 10ml K1144 erythrocytes 0, D-negative Saline; slide/spin tube Storage solution red blood cells order description 10ml K1150 Storage solution for erythrocytes as mentioned above Red blood cells for anti-human globulin control Coombs control cells are 3% red cell suspensions with blood group 0, D-positive and are sensitised with anti-d IgG antibodies. order description 10ml K1145 Coombs control cells DAT/IAT; spin tube 10ml K1138 Coombs control cells, strong sensitised ; DAT=Direct antiglobulin test DAT/IAT; spin tube Red blood cells for antibody detection 2 cell screening panel order description 2x10ml K1388 Screening set 1+2 IAT; spin tube 3 cell screening panel (from Bga-, Bgb- and Bgc negative donors) order description 3x10ml K1148 Screening panel 123 IAT; spin tube Red blood cells for the detection of Wra-antibodies order description 3ml K1393 Wra + cells IAT; spin tube Red blood cells for antibody identification In each Sanquin identification panel we offer a k-negative cell, R2RZ cell, Kpa-positive cell, Lua-positive cell and one Wra-positive cell. 16 cell identification panel order description 16x3ml K1385 Makropanel 16 panel of 16x3ml red cell suspension IAT; spin tube

5 16x3ml K1384 Makropanel 16P panel of 16x3ml Papain treated cels IAT; spin tube Column Screen - and Identification panels (0.8% cell suspension) Donor red blood cells for antibody detection in column agglutination systems (not for use in Cellbind!). 3x10ml K5010 Column Screen 123. Three ready-for-use red cells suspensions (from Bga-, Bgb- and Bgc negative donors) for the detection of unknown erythrocyte antibodies 16x3ml K5020 Column Panel 16. Sixteen ready-for-use red cell suspensions (from 16 donors) for the identification of unknown erythrocyte antibodies

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