Generating Forensic DNA Profiles
|
|
|
- Benedict Lawson
- 6 years ago
- Views:
Transcription
1 Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences Generating Forensic DNA Profiles Dan E. Krane Wright State University - Main Campus, dan.krane@wright.edu Follow this and additional works at: Part of the Biology Commons, Medical Sciences Commons, and the Systems Biology Commons Repository Citation Krane, D. E. (2012). Generating Forensic DNA Profiles.. This Presentation is brought to you for free and open access by the Biological Sciences at CORE Scholar. It has been accepted for inclusion in Biological Sciences Faculty Publications by an authorized administrator of CORE Scholar. For more information, please contact corescholar@
2 Generating forensic DNA profiles Dan E. Krane, Wright State University, Dayton, OH Forensic DNA Profiling Video Series Forensic Bioinformatics (
3 Three generations of DNA testing RFLP AUTORAD Allele = BAND DQ-alpha TEST STRIP Allele = BLUE DOT Automated STR ELECTROPHEROGRAM Allele = PEAK
4 Two additional DNA tests Mitochondrial DNA mtdna sequence Sensitive but not discriminating Y-STRs Useful with mixtures Paternally inherited
5 DNA content of biological samples Trillions of cells Roughly 100 cells Each cell contains 6 to 7 pg of DNA DNA profiling kits generally recommend using between 500 and 1,000 pg of template DNA That works out to roughly 100 to 200 cells
6 What is a picogram? 1 gram = 1/4 th of a packet of sugar 1 milligram = a single crystal of sugar 1 nanogram = one 1000 th of a crystal of sugar 1 picogram = one billionth of a gram
7 What is a microliter? 1 liter = half of a bottle of a soft drink 1 milliliter = 1000 th of a liter (about a thimble full) 100 microliters = one drop 1 microliter = one millionth of a liter
8 Basic terminology: Genetics DNA Polymorphism ( many forms ) Regions of DNA which differ from person to person Locus (plural = loci) Site or location on a chromosome Allele Different variants which can exist at a locus
9 Basic terminology: Technology Amplification or PCR (Polymerase Chain Reaction) A technique for replicating DNA in the laboratory ( molecular Xeroxing ) Region to be amplified defined by primers Electrophoresis A technique for separating molecules according to their size
10 Automated STR Test
11 Crime Scene Samples & Reference Samples Extract and purify DNA Differential extraction in sex assault cases attempts to isolate DNA from sperm cells
12 Extract and Purify DNA Add primers and other reagents
13 Setting up an amplification Pipettors are used to transfer microliter quantities of liquids Final reaction volumes are typically 10 or 20 microliters There are no good visual clues that a solution contains DNA or that a reaction is proceeding correctly
14 PCR Amplification DNA regions flanked by primers are amplified
15 PCR Amplification Targeted regions are doubled with each round of amplification. Instead of needle in a haystack, after 28 rounds of amplification there is a needle-stack with a piece of hay. Amplified DNA fragments are fluorescently labeled.
16 The ABI 310 Genetic Analyzer
17 ABI 310 Genetic Analyzer: Capillary Electrophoresis DNA pulled towards the positive electrode DNA separated out by size: Large DNA moves slowly Small DNA moves faster Color of STR detected and recorded as it passes the detector Detector Window
18 Profiler Plus: Raw data
19 Profiler Plus DNA profile
20 Reading an electropherogram Peaks correspond to alleles D3 vwa BLUE FGA Amelogenin XX = female XY = male Red = ROX size standard Electropherogram Amelogenin D8 D21 D18 GREEN D5 D13 D7 YELLOW bps RED
21 Reading an electropherogram NUMBER OF PEAKS 1 peak = homozygous 2 peaks = heterozygous 3 or more peaks = mixed sample (?) POSITION OF PEAK Smaller alleles on left Larger alleles on right HEIGHT OF PEAK Proportional to amount of allele S M A L L L A R G E
22 Profiler Plus D3S1358 vwa FGA AMEL D8S1179 D21S11 D18S51 D5S818 D13S317 D7S820
23 SGM+ D3S1358 vwa D16S539 D2S1338 AMEL D8S1179 D21S11 D18S51 D19S433 THO1 FGA
24 Profiler Plus DNA profile
25 What weight should be given to a DNA profile match? Do they have the same source? Is the match a coincidence? Has an error occurred?
26 Generating forensic DNA profiles Dan E. Krane, Wright State University, Dayton, OH Forensic DNA Profiling Video Series Forensic Bioinformatics (
STR Profiling Matching Criteria: Establishment and Importance of a Cell Line Database
STR Profiling Matching Criteria: Establishment and Importance of a Cell Line Database Margaret Kline Applied Group Biochemical Science Division Chemical Science and Technology Laboratory National Institute
Mutations during meiosis and germ line division lead to genetic variation between individuals
Mutations during meiosis and germ line division lead to genetic variation between individuals Types of mutations: point mutations indels (insertion/deletion) copy number variation structural rearrangements
Procedure for Casework DNA Interpretation
Procedure for Casework DNA Interpretation 1.0 Purpose The purpose of this document is to provide guidelines for the interpretation of autosomal DNA results when amplified with Identifiler Plus. 2.0 Scope
Essential Elements of a Defense-Review of DNA Testing Results
Wright State University CORE Scholar Biological Sciences Faculty Publications Biological Sciences 10-11-2006 Essential Elements of a Defense-Review of DNA Testing Results Dan E. Krane Wright State University
FORENSIC GENETICS. DNA in the cell FORENSIC GENETICS PERSONAL IDENTIFICATION KINSHIP ANALYSIS FORENSIC GENETICS. Sources of biological evidence
FORENSIC GENETICS FORENSIC GENETICS PERSONAL IDENTIFICATION KINSHIP ANALYSIS FORENSIC GENETICS Establishing human corpse identity Crime cases matching suspect with evidence Paternity testing, even after
Basic Steps of the DNA process
As time pasted technology has improve the methods of analyzing DNA. One of the first methods for the analysis of DNA is known as Restriction Fragment Length Polymorphism (RFLP). This technique analyzed
Procedure for GeneMapper ID-X for Casework
Procedure for GeneMapper ID-X for Casework 1.0 Purpose-This procedure specifies the steps for performing analysis on DNA samples amplified with AmpFlSTR Identifiler Plus using the GeneMapper ID-X (GMID-X)
Data Quality Worth Sharing
Product Bulletin Human Identification AmpFlSTR NGM and NGM SElect PCR Amplification Kits Next generation amplification chemistries including the 5 new loci from the expanded European Standard Set Enhanced
AmpF STR NGM PCR Amplification Kit - Overview
AmpF STR NGM PCR Amplification Kit - Overview The most advanced STR kits optimized for analysis of forensic casework and database samples in Europe Data Quality Worth Sharing! 9/7/2011 Life Technologies
The Evolution of Short Tandem Repeat (STR) Multiplex Systems
Ann M. Lins, Cynthia J. Sprecher, Katherine A. Micka, Dawn R. Rabbach, Jennifer A. Taylor, Jeffrey W. Bacher, Laura F. Hennes, Allan Tereba, Trent Gu, Elizabeth A. Amiott, Nadine A. Nassif and James W.
John M. Butler and Peter M. Vallone National Institute of Standards and Technology
DNA Biometrics: i Standards and Technology John M. Butler and Peter M. Vallone National Institute of Standards and Technology NDIA Biometrics Conference (Arlington VA) NDIA Biometrics Conference (Arlington,
Laboratory Exercise 4. Multiplex PCR of Short Tandem Repeats and Vertical Polyacrylamide Gel Electrophoresis.
Laboratory Exercise 4 4 Multiplex PCR of Short Tandem Repeats and Vertical Polyacrylamide Gel Electrophoresis B A C K G R O U N D The human genome contains over 3000 million base pairs, which are distributed
Authenticating human cell lines using the Identifiler kits and capillary electrophoresis platforms
APPLICATION NOTE Identifiler kits and SeqStudio Genetic Analyzer Authenticating human cell lines using the Identifiler kits and capillary electrophoresis platforms In this application note, we show: The
Internal Validation of the Promega PowerPlex Fusion System with the Applied Biosystems 3130xl Genetic Analyzer
Internal Validation of the Promega PowerPlex Fusion System with the Applied Biosystems 3130xl Genetic Analyzer Roy Al Ahmar, B.S. Marshall University Forensic Science Center DNA Laboratory Overview What
4.1. Genetics as a Tool in Anthropology
4.1. Genetics as a Tool in Anthropology Each biological system and every human being is defined by its genetic material. The genetic material is stored in the cells of the body, mainly in the nucleus of
Further Reading - DNA
Further Reading - DNA DNA BACKGROUND What is DNA? DNA (short for deoxyribonucleic acid ) is a complex molecule found in the cells of all living things. The blueprint for life, DNA contains all the information
AUTOMATION OF DNA ISOLATION, QUANTITATION, AND PCR SETUP
AUTOMATION OF DNA ISOLATION, QUANTITATION, AND PCR SETUP Paraj Mandrekar, Laura Flanagan, Robert McLaren and Allan Tereba Promega Corporation, Madison, WI Abstract As a result of increased casework due
Short Tandem Repeat (STR) Analysis
Maj Gen (R) Suhaib Ahmed, HI (M) Short tandem repeats (STR) are randomly distributed DNA sequences in which 2-6bp are tandemly repeated. These are scattered on all chromosomes including the autosomes as
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR Ref. PCR1 1. OBJECTIVE OF THE EXPERIMENT The objective of this experiment is to introduce students to the principles and practice of Polymerase Chain Reaction (PCR)
AmpFlSTR COfiler PCR Amplification Kit
USER BULLETIN AmpFlSTR COfiler PCR Amplification Kit Publication Number 4306116 Rev. H Revision Date August 2012 SUBJECT: Kit Overview, Developmental Validation, and Performance Validation Product overview......................................................
The effect of the BlueStar blood reagent on DNA typing.
FRENCH DEFENSE DEPARTMENT GENDARMERIE NATIONALE CRIMINAL RESEARCH INSTITUTE BIOLOGY DEPARTMENT The effect of the BlueStar blood reagent on DNA typing. BLUESTAR 2001 by Aspirant BRENIAUX CONTENTS INTRODUCTION.
An Introduction to Forensic DNA Analysis
2/7/2013 HC70A: Genetic Engineering in Medicine, Agriculture & Law 1 UCLA Dr. Bob Goldberg HC70A: Genetic Engineering in Medicine, Agriculture & Law An Introduction to Forensic DNA Analysis Presented by
User Bulletin. Veriti 96-Well Thermal Cycler AmpFlSTR Kit Validation. Overview
User Bulletin Veriti 96-Well Thermal Cycler AmpFlSTR Kit Validation June 2009 SUBJECT: AmpFlSTR PCR Amplification Kit validation on the Veriti 96-Well Thermal Cycler with 0.2 ml sample block format In
TWGDAM Validation of AmpF STR PCR Amplification Kits for Forensic DNA Casework
Cydne L. Holt, 1 Ph.D.; Martin Buoncristiani, 2 M.P.H.; Jeanette M. Wallin, 1 M.P.H.; Theresa Nguyen, 1 B.S.; Katherine D. Lazaruk, 1 Ph.D.; and P. S. Walsh, 1 M.P.H. TWGDAM Validation of AmpF STR PCR
AGENDA for 10/11/13 AGENDA: HOMEWORK: Due end of the period OBJECTIVES:
AGENDA for 10/11/13 AGENDA: 1. Finish 1.2.3 DNA Analysis Analyzing DNA Samples Using Current Forensic Methods OBJECTIVES: 1. Demonstrate the steps of gel electrophoresis 2. Analyze restriction fragment
The Polymerase Chain Reaction. Chapter 6: Background
The Polymerase Chain Reaction Chapter 6: Background PCR Amplify= Polymerase Chain Reaction (PCR) Invented in 1984 Applications Invention of PCR Kary Mullis Mile marker 46.58 in April of 1983 Pulled off
PCR Laboratory Exercise
PCR Laboratory Exercise Advance Protocol (updated 1/2018) Introduction Detection of TPA-25 Alu by PCR A Human DNA Fingerprinting Lab Protocol 1994 Cold Spring Harbor Laboratory DNA Learning Center In this
Unit 2- DNA Analysis
Unit 2- DNA Analysis Discovery of DNA structure 1950 s Rosalind Franklin & Maurice Wilkins photograph DNA using x-ray diffraction 1 Discovery of DNA structure 1953 James Watson & Francis Crick develop
AGENDA for 10/10/13 AGENDA: HOMEWORK: Due end of the period OBJECTIVES: Due Fri, 10-11
AGENDA for 10/10/13 AGENDA: 1. 1.2.3 DNA Analysis Analyzing DNA Samples Using Current Forensic Methods OBJECTIVES: 1. Demonstrate the steps of gel electrophoresis 2. Analyze restriction fragment length
Chapter 7 DNA Fingerprinting By the end of this chapter you will be able to:
Chapter 7 DNA Fingerprinting By the end of this chapter you will be able to: explain how crime scene evidence is collected and processed to obtain DNA describe how radioactive probes are used in DNA fingerprinting
The Real CSI: Using DNA to Identify Criminals and Missing Persons
The Real CSI: Using DNA to Identify Criminals and Missing Persons San Jose State University May 2, 2012 Overview Forensic DNA in the media perceptions and reality The power and limitations of nuclear (STR)
Genetics module. DNA Structure, Replication. The Genetic Code; Transcription and Translation. Principles of Heredity; Gene Mapping
Genetics module Lectures DNA Structure, Replication The Genetic Code; Transcription and Translation Principles of Heredity; Gene Mapping Controlling Gene Expression Mutation and Cancer Textbook: Introduction
This is a typical chromatogram generated by automated sequencing.
DNA TECHNOLOGY AND FORENSICS Introduction: DNA (Deoxyribonucleic Acid) is a molecule that is the main part of your chromosomes, which carry your hereditary material. The molecule is shaped like a twisted
STR Interpretation Guidelines
Introduction: STR Interpretation Guidelines The interpretation of results in casework is necessarily a matter of professional judgment and expertise. Not every situation can or should be covered by a pre-set
GenPlex HID Training Class I
Rixun Fang GenPlex HID Training Class I Outline of Presentation Introduction GenPlex HID kit Experimental plan Class schedule Forensic SNP Analysis GenPlex HID Training Class I 2 Potential Forensic Applications
Uniparental disomy (UPD) analysis of chromosome 15
YOUR INNOVATIVE RESEARCH Analysis of UPD by STR-PCR Uniparental disomy (UPD) analysis of chromosome 15 Applied Biosystems 3500xL Genetic Analyzer Introduction Researchers at the Laboratory of Medical Genetics
Population Genetics (Learning Objectives)
Population Genetics (Learning Objectives) Define the terms population, species, allelic and genotypic frequencies, gene pool, and fixed allele, genetic drift, bottle-neck effect, founder effect. Explain
The Polymerase Chain Reaction. Chapter 6: Background
The Polymerase Chain Reaction Chapter 6: Background Invention of PCR Kary Mullis Mile marker 46.58 in April of 1983 Pulled off the road and outlined a way to conduct DNA replication in a tube Worked for
DNA analysis. Anja Bye Post doktor. K.G. Jebsen Senter for Hjertetrening. Institutt for Sirkulasjon og Bildediagnostikk Det Medisinske Fakultet NTNU
DNA analysis Anja Bye Post doktor K.G. Jebsen Senter for Hjertetrening Institutt for Sirkulasjon og Bildediagnostikk Det Medisinske Fakultet NTNU Focus of this lecture What is DNA? Comparing DNA from different
Comparison of the Promega Power Y and AB AmpFlSTR YFiler kits and the Subsequent Validation of YFiler.
Comparison of the Promega Power Y and AB AmpFlSTR YFiler kits and the Subsequent Validation of YFiler. Dr. Timothy P McMahon Armed Forces DNA Identification Laboratory Rockville, Md 301-319 319-0238 Timothy.mcmahon@afip.osd.mil
PowerPlex ESX 17 Fast System for Use on the Applied Biosystems Genetic Analyzers
TECHNICAL MANUAL PowerPlex ESX 17 Fast System for Use on the Applied Biosystems Genetic Analyzers Instructions for Use of Products DC1710 and DC1711 Revised 8/17 TMD040 PowerPlex ESX 17 Fast System for
Latest Tools for Human Identification
Latest Tools for Human Identification Lisa Calandro, Senior Forensic Scientist Applied Markets Division To hear the audio portion of this seminar, please call the phone number for your country: Australia-1800002092
Manipulating DNA. Nucleic acids are chemically different from other macromolecules such as proteins and carbohydrates.
Lesson Overview 14.3 Studying the Human Genome Nucleic acids are chemically different from other macromolecules such as proteins and carbohydrates. Nucleic acids are chemically different from other macromolecules
NRAS Codon 61 Mutation Analysis Reagents
NRAS Codon 61 Mutation Analysis Reagents User Manual V1.0 Cat No. GP19 32 reactions 1 CONTENTS Introduction 4 Overview of Mutector TM Assay 5 Materials Provided 6 Materials Required 7 Equipment Required
AmpFlSTR NGM SElect PCR Amplification Kit. User Guide
AmpFlSTR NGM SElect PCR Amplification Kit User Guide Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this
PowerPlex. Y System Validation
PowerPlex Y System Validation By Patricia M. Fulmer, Dawn Rabbach, Kimberly Huston, Curtis Knox and Cynthia Sprecher, Promega Corporation Abstract We have improved the manufacturing process for the PowerPlex
The ABCs & 123s of DNA Testing
The ABCs & 123s of DNA Testing By Jenny S Cheung & Allison Lewis Legal Aid Society DNA Unit Training for Federal Defenders October 7, 2016 1 TOPICS What is DNA How do they test DNA Discovery in a DNA case
Analyzing Y-STR mixtures and calculating inclusion statistics
Analyzing Y-STR mixtures and calculating inclusion statistics Rick W. Staub, Ph.D. and Cassie Johnson, M.S. Orchid Cellmark Inc. Dallas, TX ABSTRACT Forensic evidence samples analyzed with Y-STR multiplexes
CE and CEC. 1 Introduction. Electrophoresis 2004, 25,
Electrophoresis 2004, 25, 2227 2241 2227 Pieris Koumi Helen E. Green Susan Hartley Darren Jordan Sharon Lahec Richard J. Livett Kam W. Tsang David M. Ward The Forensic Science Service, Solihull, UK Evaluation
Genetics Lecture 16 Forensics
Genetics Lecture 16 Forensics DNA Forensics Genetics is arguably the most influential science today dramatically affecting technologies in fields as diverse as agriculture, archaeology, medical diagnosis,
Development of a Global STR Multiplex for Human Identification Analysis
Development of a Global STR Multiplex for Human Identification Analysis Jonathan Tabak Life Technologies 1 Agenda Introduction Multiplex Configuration Strategy Application-Specific Development and Testing
TECHNICAL MANUAL. GenePrint 24 System. Instructions for Use of Product B1870 6/16 TM465
TECHNICAL MANUAL GenePrint 24 System Instructions for Use of Product B1870 6/16 TM465 GenePrint 24 System All technical literature is available at: www.promega.com/protocols/ Visit the web site to verify
NRAS Mutation Analysis Reagents (Codons 12 and 13)
NRAS Mutation Analysis Reagents (Codons 12 and 13) User Manual V1.1 Cat No. GP18 32 reactions 1 CONTENTS Introduction 4 Overview of Mutector TM Assay 5 Materials Provided 6 Materials Required 7 Equipment
Maximizing the Performance of Capillary Electrophoresis Systems The Maximizing Data Quality Series Part 4
Maximizing the Performance of Capillary Electrophoresis Systems The Maximizing Data Quality Series Part 4 Introduction Since the introduction of the ABI PRISM 310 Genetic Analyzer in 1996, capillary electrophoresis
Investigator Quantiplex HYres Kit
These specifications are for the procurement of a validation study at GBI Division of Forensic Sciences facilities of two commercially available kits used in forensic DNA analysis. The two kits to be validated
Lecture Four. Molecular Approaches I: Nucleic Acids
Lecture Four. Molecular Approaches I: Nucleic Acids I. Recombinant DNA and Gene Cloning Recombinant DNA is DNA that has been created artificially. DNA from two or more sources is incorporated into a single
Sequence structure of 12 novel Y chromosome microsatellites and PCR amplification strategies
International Congress Series 1239 (2003) 425 429 Sequence structure of 12 novel Y chromosome microsatellites and PCR amplification strategies Anna González-Neira a, Paula Sánchez-Diz a, Leonor Gusmão
SeCore SBT Sequence Based Typing
MOLECULAR TYPING Product Brochure SeCore SBT Sequence Based Typing Key Benefits SeCore HLA typing kits combine the accuracy of bidirectional sequencing and the power and flexibility of our improved sequence
Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit
Product Bulletin Human Identification Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit The Quantifiler kits produce reliable and reproducible results, helping to
PCR Techniques. By Ahmad Mansour Mohamed Alzohairy. Department of Genetics, Zagazig University,Zagazig, Egypt
PCR Techniques By Ahmad Mansour Mohamed Alzohairy Department of Genetics, Zagazig University,Zagazig, Egypt 2005 PCR Techniques ISSR PCR Inter-Simple Sequence Repeats (ISSRs) By Ahmad Mansour Mohamed Alzohairy
Sanger Sequencing & Fragment Analysis Services à la carte
Sanger Sequencing & Fragment Analysis Services à la carte www.seqme.eu SEQme 2017 All rights reserved The trademarks and names of other companies and products mentioned in this brochure are the property
Laboratory Validation. Chapter 16
Laboratory Validation Chapter 16 Importance The DNA profile is used to convict or free a suspect It must be perfect! No mistakes like we have in regular laboratories all the time Also DNA evidence must
Forensic DNA analysis
FORENSIC DNA ANALYSIS: A PRIMER FOR COURTS 1 Forensic DNA analysis A PRIMER FOR COURTS 2 FORENSIC DNA ANALYSIS: A PRIMER FOR COURTS Forensic DNA analysis: a primer for courts Issued: November 2017 DES4928
The use of mitochondrial DNA and short tandem repeat typing in the identification of air crash victims. Nucleic acids.
Electrophoresis 1999, 20, 1707±1711 1707 William Goodwin 1 Adrian Linacre 2 Peter Vanezis 1 1 Human Identification Centre, Department of Forensic Medicine and Science, University of Glasgow, Glasgow, UK
Molecular Markers CRITFC Genetics Workshop December 9, 2014
Molecular Markers CRITFC Genetics Workshop December 9, 2014 Molecular Markers Tools that allow us to collect information about an individual, a population, or a species Application in fisheries mating
LATE-PCR. Linear-After-The-Exponential
LATE-PCR Linear-After-The-Exponential A Patented Invention of the Laboratory of Human Genetics and Reproductive Biology Lab. Director: Lawrence J. Wangh, Ph.D. Department of Biology, Brandeis University,
ON-CHIP AMPLIFICATION OF GENOMIC DNA WITH SHORT TANDEM REPEAT AND SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS
ON-CHIP AMPLIFICATION OF GENOMIC DNA WITH SHORT TANDEM REPEAT AND SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS David Canter, Di Wu, Tamara Summers, Jeff Rogers, Karen Menge, Ray Radtkey, and Ron Sosnowski Nanogen,
MRC-Holland MLPA. Description version 12; 27 November 2015
SALSA MLPA probemix P079-A3 OTC Lot A3-1015. As compared to previous version (lot A2-0211), 5 reference probes have been replaced. Also, 8 reference probes and the TSPAN7 flanking probe have been removed.
Simplified Low Copy Number Dna Analysis By Post Pcr Purification
University of Central Florida Electronic Theses and Dissertations Masters Thesis (Open Access) Simplified Low Copy Number Dna Analysis By Post Pcr Purification 2006 Pamela Smith University of Central Florida
The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters.
Evaluation of forensic DNA mixture evidence: protocol for evaluation, interpretation, and statistical calculations using the combined probability of inclusion The Harvard community has made this article
DNA Mixture Interpretation Workshop Michael D. Coble, PhD. Current SWGDAM Guidelines
DNA Mixture Interpretation Workshop Michael D. Coble, PhD Current SWGDAM Guidelines Hierarchy of Rules for Forensic DNA Labs Validation Audits United States FBI (DAB) Quality Assurance Standards NDIS Procedures
Chapter 10 Analytical Biotechnology and the Human Genome
Chapter 10 Analytical Biotechnology and the Human Genome Chapter Outline Enzyme tests and biosensors DNA-based tests DNA analysis technologies Human genome and genome-based analytical methods 1 Enzyme-based
EVALUATION OF THEPRECISIONID SYSTEM FOR TARGETED SEQUENCING OF DNA AND RNA MARKERS FOR HUMAN IDENTITY AND BODY FLUID IDENTIFICATION
EVALUATION OF THEPRECISIONID SYSTEM FOR TARGETED SEQUENCING OF DNA AND RNA MARKERS FOR HUMAN IDENTITY AND BODY FLUID IDENTIFICATION www. tourisminbarcelona.com Jack Ballantyne HIDS, Barcelona, Spain 2016
Mentype DIPscreen Manual
Mentype DIPscreen Manual The entry to a quantitative chimerism analysis In-Vitro-Diagnostics 25 100 400 Version July 2015 45-45410-0025 45-45410-0100 45-45410-0400 Batch Code Biotype Diagnostic GmbH Moritzburger
Validation of PowerPlex Y23 PCR Amplification System for Forensic Casework
Validation of PowerPlex Y23 PCR Amplification System for Forensic Casework Jessica Tokarz (B.S.) 1, Sarah Foster (B.S.) 2, Scott Milne (B.S.) 2, Pamela Staton (PhD) 1, Kathleen Press (M.S.F.S.) 3 1 Marshall
BotanoTech. S c ience N o t ebook. Comparative Plant Genomics Study
BotanoTech S c ience N o t ebook Comparative Plant Genomics Study Table of Contents Introduction & Question... 2 Page Research Phenotype...... 3 Hypothesis. 4 Materials & Methods. 5 Part I Genomic DNA
HiPer Real-Time PCR Teaching Kit
HiPer Real-Time PCR Teaching Kit Product Code: HTBM032 Number of experiments that can be performed: 10 Duration of Experiment Protocol: 1.5 hours Storage Instructions: The kit is stable for 12 months from
PV92 PCR Bio Informatics
Purpose of PCR Chromosome 16 PV92 PV92 PCR Bio Informatics Alu insert, PV92 locus, chromosome 16 Introduce the polymerase chain reaction (PCR) technique Apply PCR to population genetics Directly measure
Q1 (1 point): Explain why a lettuce leaf wilts when it is placed in a concentrated salt solution.
Short questions 1 point per question. Q1 (1 point): Explain why a lettuce leaf wilts when it is placed in a concentrated salt solution. Answer: Water is sucked out of the cells by osmosis (this reduces
BC2004 Review Sheet for Lab Exercises 7-11 Spring Semester 2005
BC2004 Review Sheet for Lab Exercises 7-11 Spring Semester 2005 Lab Exercise 7 Drosophila crosses, three weeks Vocabulary: phenotype, genotype, gene, allele, locus (loci), sex chromosomes, autosomes, homozygous,
Book chapter appears in:
Mass casualty identification through DNA analysis: overview, problems and pitfalls Mark W. Perlin, PhD, MD, PhD Cybergenetics, Pittsburgh, PA 29 August 2007 2007 Cybergenetics Book chapter appears in:
1a. What is the ratio of feathered to unfeathered shanks in the offspring of the above cross?
Problem Set 5 answers 1. Whether or not the shanks of chickens contains feathers is due to two independently assorting genes. Individuals have unfeathered shanks when they are homozygous for recessive
minipcr Forensics Lab: Analysis of the D1S80 VNTR
minipcr Forensics Lab: Analysis of the D1S80 VNTR Instructor s Guide Contents 1. Synopsis p 2 2. Learning goals and skills developed p 3 3. Standards alignment p 4 4. Scenario overview p 6 5. Laboratory
Lesson 3 Gel Electrophoresis of Amplified PCR Samples and Staining of Agarose Gels
Lesson 3 Gel Electrophoresis of Amplified PCR Samples and Staining of Agarose Gels What Are You Looking At? Before you analyze your PCR products, let s take a look at the target sequence being explored.
Notice of Amendment of the FBI s STR Population Data Published in 1999 and 2001
Notice of Amendment of the FBI s STR Population Data Published in 1999 and 2001 Recently, new amplification kits that expand the number of loci in a multiplex reaction have become commercially available.
Absolute Human Telomere Length Quantification qpcr Assay Kit (AHTLQ) Catalog # reactions
Absolute Human Telomere Length Quantification qpcr Assay Kit (AHTLQ) Catalog #8918 100 reactions Product Description Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect
rjlflemmers, LUMC, Leiden, The Netherlands 6/3/2010
Genotyping of the SSLP The simple sequence length polymorphism (SSLP) 3.5 kb proximal to D4Z4 is studied by PCR and the sequence is localized between positions 1532 and 1694 of AF117653 (see figure below).
Assessment of DNA transfer Involving Routine Human Behavior
University of North Texas Health Science Center UNTHSC Scholarly Repository Theses and Dissertations 5-2010 Assessment of DNA transfer Involving Routine Human Behavior Shamika Kelley University of North
Bio Rad PCR Song Lyrics
Bio Rad PCR Song Lyrics There was a time when to amplify DNA, You had to grow tons and tons of tiny cells. (Oooh) Then along came a guy named Dr. Kary Mullis, Said you can amplify in vitro just as well.
Genetic Fingerprinting
Genetic Fingerprinting Introduction DA fingerprinting In the R & D sector: -involved mostly in helping to identify inherited disorders. In forensics: -identification of possible suspects involved in offences.
Introduction. Thomas Hunt Morgan. Chromosomes and Inheritance. Drosophila melanogaster
Chromosomes and Inheritance 1 4 Fig. 12-10, p. 244 Introduction It was not until 1900 that biology finally caught up with Gregor Mendel. Independently, Karl Correns, Erich von Tschermak, and Hugo de Vries
RFLP: Restriction Fragment Length Polymorphism
RFLP: Restriction Fragment Length Polymorphism Various endonucleases: 6 cutters and 4 cutters Enzyme Source Recognition Sequence Cut EcoRI Escherichia coli 5'GAATTC 5'---G/AATTC---3' EcoRII Escherichia
Session 3 Cloning Overview & Polymerase Chain Reaction
Session 3 Cloning Overview & Polymerase Chain Reaction Learning Objective: In this lab exercise, you will become familiar with the steps of a polymerase chain reaction, the required reagents for a successful
JS 190- Population Genetics- Assessing the Strength of the Evidence Pre class activities
JS 190- Population Genetics- Assessing the Strength of the Evidence I. Pre class activities a. Quiz then Review Assignments and Schedule II. Learning Objectives a. Overview of Validation Developmental
Certificate of Analysis of the Holotype HLA 24/7 Configuration A1 & CE
Certificate of Analysis of the Holotype HLA 24/7 Configuration A1 & CE Product name Holotype HLA 24/7 Configuration A1 & CE Reference number H52 LOT number 00027 (N4/002-P5/006-E1/004-R2/006) Expiration
Introduction to PCR. Kristen Wolslegel Manager, Education Programs BABEC
Introduction to PCR Kristen Wolslegel Manager, Education Programs BABEC What is DNA? It encodes within its structure the hereditary information that determines the form and function of all known living
Genes and human health - the science and ethics
Deoxyribonucleic acid (DNA) - why is it so important? Genes and human health - the science and ethics DNA is essential to all living organisms, from bacteria to man, as it contains a code which specifies
GENETICS: BIOLOGY HSA REVIEW
GENETICS: BIOLOGY HSA REVIEW HSA Review A. Matching: On the lines provided, write the letter of the definition of each term. a. genetics f. gamete b. trait g. probability c. hybrid h. Punnett square d.