LabChip 90 System with DataViewer Software

Transcription

1 Revolutionizing DNA and Protein Gel Electrophoresis LabChip 90 System with DataViewer Software The Proven Alternative to Slab Gels. The massive flow of information emerging from today s genomic and proteomic research has created an urgent need for more effective ways to generate and analyze data. DNA and protein analysis has traditionally been performed using slab gel electrophoresis. However, agarose slab gel and SDS-PAGE methodologies are time-consuming, labor-intensive and prone to variability. The LabChip 90 System offers researchers an automated alternative by streamlining the multiple, manual steps of slab gel electrophoresis, while also providing the throughput and data quality essential for life science laboratories today.

2 LabChip Technology Advantages of LabChip Technology Automated Sampling from 96- and 384-Well Plates Transfer plates from a thermal cycler directly into the LabChip 90 System, no sample prep or transfer needed Less Hands-on Time Reduces the manual labor associated with gels and frees up valuable resources Superior Data Quality More precise, accurate and reproducible data than gels Faster Time to Result Quantitative sizing and concentration data are automatically reported as each sample is analyzed Direct Generation of Digital Data Eliminates the need for photodocumentation Versatility DNA and protein analysis can be performed using the same system Marker Well Higher Sample Throughput Complete analysis for hundreds of samples in just a few hours; no further processing required LabChip Electrophoresis Vacuum Well Sipper DNA Chip. The blue wells are filled with a mixture of sieving polymer and fluorescent dye. The green well contains internal DNA markers. Sampling. Vacuum is applied. This pulls the sample onto the chip through the sipper, and also draws the internal DNA markers to mix with the sample. How Does it Work? LabChip electrophoresis is performed on a small, microfluidic chip. Prior to DNA or protein analysis, reagents are loaded into the individual wells of the chip. These wells are connected to small plates of quartz etched with tiny microchannels about the size of a human hair. When the chip is loaded into the LabChip 90 System, its wells interface with platinum electrodes that provide voltage and current control. The system robot moves the microtiter plate wells directly under the chip s capillary sipper, and approximately 150 nl of sample is aspirated onto the chip. Individual sample analytes are separated electrophoretically and the bands are detected via laserinduced fluorescence. Sizing and concentration for each band are determined using both a ladder and internal markers. Because the sipper is rinsed between samples, cross-contamination or carryover is eliminated. Markers flow toward the Vacuum Well Detection Point Separation Channel Injection Intersection Injection. Voltage drives the samplemarker mix across the injection intersection where a pinch current is applied to inject a small plug into the separation channel. Separation. Voltage is applied to perform electrophoresis in the separation channel. The individual DNA fragments are stained and then separated based on size. Separated Bands Let Us Make Your LabChip 90 Data Review and Reporting Easier DataViewer makes it so easy to compare data from multiple LabChip 90 sample plates. Compare wells within a plate, across multiple plates, even wells from 96 well plates against 384 well plates! Developed with ease of use in mind, DataViewer provides multiple analytical functions, selectable at the click of a tab. Data can be view as a Virtual Gel, Graph, or Data Summary Table. The DataViewer software is the key to building digital summaries of experimental results, which can be exported and captured in reports, or shared with colleagues.

3 DataViewer DNA and Protein Analysis Software DataViewer allows users to compare data from several different sample plates. Users can compare wells within a plate, across multiple plates, even wells from 96 well plates against 384 well plates! As with the current HT software, data can be displayed in Virtual Gel, Graph, or Data Summary Table formats. Users can either manually select the wells of interest (wells, rows, columns, plates) or use an automated selection function to select wells by data result (size, peak height, concentration, etc.) Data screening templates can be stored for use on subsequent analyses. Data 'collections' which are produced by a particular analysis can be saved and exported for future reference, or for sharing with colleagues. Import LabChip 90 Data Files With Ease Use Filter Functions to Analyze Data The DataViewer Filter Function allows for results to be automatically derived by any of seven variables. Filters can be combined by easily selected logic operations. Filters can be named and stored for reuse as templates. Each plate well with at least one data peak that corresponds to the filter set is highlighted for easy observation, and the data are displayed in the Gel, Graph, and Table tabs. Open multiple data files and compare results in either a traditional gel view, or use electropherograms. Your choice of data display is only a click away. DataViewer operates on standard CLA files generated by LabChip HT. Export Graphics or Tables Export functions are built in to make it easy to capture the digitized data in reports, or share with colleagues. Peak Tables export to a CSV format text file. Text files are a convenient means to pull data into a wide variety of lab applications. DataViewer is ideal for: Comparing multiple LabChip 90 data files Filtering LabChip 90 data by variable and value range Exporting formatted results tables to reports or LIMS

4 LabChip HT Software Quantitative Results in Seconds LabChip HT Software is a far more precise, accurate and reliable method of generating data than gel scanning and photodocumentation. The LabChip 90 System automatically calculates the size and concentration of each protein or DNA fragment and provides digital results. Data are generated in just seconds per sample and are reported real-time as each sample is processed. Results are displayed in a tabular format, an electropherogram view and in a gel-like image. The virtual gel image resembles traditional slab gel results and automatically aligns lanes to make visual comparison easier Multiple gel views allow you to see a row of samples or the entire 96- or 384-well plate Start a run anywhere on the plate or select specific subsections of the plate for analysis Flag up to ten expected proteins or DNA fragments per sample Data overlay capabilities make it easy to compare samples within a plate Gel images, electropherograms and results tables can be copied and pasted into reports and presentations Switch between DNA and protein analysis easily by selecting one of several preconfigured assays Automatically export tabulated results or raw data in text format Import sample information prior to starting analysis Plate bar codes can be scanned and stored with the data file

5 DNA Analysis RFLP DNA inserts were amplified, restriction digested and analyzed using the LabChip DNA assay and a 4% agarose slab gel. The high quality of data generated by the LabChip 90 System is well suited for automated DNA fingerprinting assays. Protein Analysis Crude Lysates For comparative purposes, crude lysate samples were analyzed using the LabChip protein assay and using an 8-16% SDS-PAGE gradient gel. As shown in the virtual gel image, the LabChip 90 System data are highly reproducible and the bands of interest are better resolved kda 48 kda Top - LabChip 90 System data. The green bands in the LabChip 90 System virtual gel image are upper and lower internal markers. Bottom - 4% agarose gel image. Samples, LabChip 90 System data and agarose gel image provided by BD Pharmingen/Dyax Corporation, San Diego, CA. Left LabChip 90 System virtual gel image. Expressed protein size reported at 47.7 kda. Right - SDS-PAGE gel image. Expressed protein at 48 kda. Samples, LabChip 90 System and SDS-PAGE data provided by Structural GenomiX, San Diego, CA. PCR Products PCR fragments were analyzed on the LabChip 90 System and on different concentrations of agarose gels. The LabChip 90 System easily resolved the 140 bp and 210 bp fragments as well as the 400 bp and 420 bp fragment pair. The slab gels were not able to achieve this resolution. Antibodies Alternating samples of reduced and non-reduced IgG antibody were analyzed. The data quality of the LabChip 90 System makes it ideal for QC monitoring of antibody production. + Left LabChip 90 System PCR fragment data. The virtual gel image also displays the DNA assay ladder that is sipped prior to each row of samples. Right 0.8% and 2% agarose gel images: lane 1 LabChip DNA assay ladder, lane 2 PCR fragments, lane 3 - PhiX174/ Hinf1. The red band on the virtual gel image is the internal marker and the dark blue band is an SDS peak. Sizing and concentration data for each protein can be viewed by rolling the cursor over the band of interest.

6 LabChip 90 System Assays Precision DNA and Protein Analysis Because sample loading, injection, and separation are precisely controlled on the chip, LabChip electrophoresis provides highly accurate analytical data. Lane-to-lane and plate-to-plate results therefore are extremely reproducible. LabChip assays cover a wide variety of comparable gel concentrations, so a much broader sample sizing range can be achieved using just one DNA or protein assay. Fragments close in size that are often presented as broad bands on slab gels are distinctly resolved using the LabChip assays. Low concentrations of DNA or protein in the presence of other, more concentrated species, are easily detected. Since only nanoliters of sample are used, the remaining sample can be used for other experiments. The LabChip 90 System can analyze up to two DNA samples or one protein sample in about a minute. LabChip kits come with one chip and all the reagents needed to perform analysis of either protein or DNA samples.

7 Specifications LabChip 90 System Plate Formats 96- and 384-well Dimensions 147 cm (58 in) H x 57 cm (22.5 in) W x 61 cm (24 in) D Excitation/Emission Wavelengths 635 and 700 nm Weight 136 kg (300 lbs) Power requirements V AC, 47/63 Hz Power consumption 360 VA (360 W) Operating humidity range 5 85% relative, noncondensing Operating temperature range C ( F) DataViewer Analysis Software System Requirements The minimum PC requirements are as follows: Pentium II processor 400 MHz or higher Windows NT Operating System, Service Service Pack 6 or Windows 2000 Service Pack Operating System 128 MB RAM 500 MB free hard disk space CD ROM drive SVGA display monitor, 1024 x 768, 256 colors IE 5.5 or later Network Interface Card Ordering Information LabChip 90 with LabChip HT and DataViewer Software DataViewer Analysis Software Bar Code Scanner Option HT DNA 12K LabChip Kit HT DNA 5K SE30 LabChip Kit HT DNA 5K SE55 LabChip Kit HT Protein 200 LabChip Kit

8 68 Elm Street Hopkinton, MA Tel: Caliper Life Sciences, Inc. All rights reserved. Caliper, the Caliper logo and DataViewer are tradenames and/or trademarks of Caliper Life Sciences, Inc. LC90-BR-01 Jul07