THE CELLULAR AND MOLECULAR BASIS OF INHERITANCE
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1 Umm AL Qura University THE CELLULAR AND MOLECULAR BASIS OF INHERITANCE Dr. Neda Bogari
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4 EMERY'S ELEMENTS OF MEDICAL GENETICS Peter Turnpenny and Sian Ellard 13 th edition 2008
5 COURSE SYLLABUS A) Genome, Transcriptome, and Proteome. 1.DNA and DNA replication 2.Transcription 3.Translation 4.Genome gene 2.Chromosomes and cell division 1.Human chromosomes morphology and classification 2.Cell cycle division 3.Mitosis 4.Meiosis
6 WHAT IS GENETICS? Genetics is the study of heredity, or what makes organisms differ from one to another The transmission of characteristics from one generation to the next
7 GENETICS Study of inheritance How character or information are passed from one generation to the next
8 CELL The essential unit of life Trillions in each human Differentiated cells Cells that have specialized Only use a small part of genome
9 HUMAN GENOME Where we can find the DNA in the cell Nucleus Mitochondrial What is the human genome project the human genome project is a major international collaborative effort to map a sequence the entire human genome
10 NUCLEUS Contains the genetic material
11 Zygote is stem cell Genome = Transcriptome Proteome 46 cell both somatic cells and zygote contain the same DNA but their gene expressio n profile are different
12 DNA MAKES RNA MAKES PROTEIN Genome is the complete set of genetic instruction in the cell of a particular type of organism including nuclear and mitochondrial DNA. Transcriptome is the entire mrna content of the cell Proteome is the collection of functioning proteins synthesized by a living cell
13 Made of DNA and protein 23 pair Autosomes Pair 1-22 Sex chromosmes X and Y Karyotype Picture of chromsomes chromosome CHROMOSOMES is made of two sister chromatids, which are held together by the centromere
14 GENE 2 to 3 m 3X10 9 bp =3200mb 10bp 10bp 10bp Unit of inheritance Made of DNA Contain instructions for making a specific protein Heterochromatin (AT rich) Inactive Euchromatin (GC rich ) Active
15 DNA Made of building blocks Adenine (A) Thymine (T) Cytosine (C) Guanine (G) Can copy itself Is passed from one generation to another Stores genetic information
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17 Chromosomes, DNA, and Genes Cell Nucleus Chromosomes Gene Protein
18 The hereditary material is present in the nucleus of the cell. Protein synthesis takes place in the cytoplasm. What is the chain of events which leads from the gene to the final products?
19 DNA: THE HEREDITARY MATERIAL A- COMPOSITION Nucleic acid is composed of a long chain of individual molecules called nucleotides. Each nucleotide is composed of nitrogenous base, a sugar molecule and a phosphate molecule.
20 5 end phosphate 5 3 S S S S P C G A T Sugar base One nucleotide
21 STRUCTURE OF BASES Pyrimidines Purines Cytosine Thymine Uracil Adenine Guanine
22 The nitrogenous bases fall into two types, purines and pyrimidines. The Purines include adenine (A) and guanine (G). The pyrimidines include cytosine (C), thymine (T), and uracil (U).
23 sugar Phosphate Deoxyribose
24 B- STRUCTURE OF DNA DNA exists as two strands, twisted together into a right handed helix, called the double helix. Each strand is a polymer of repeating units called nucleotides. The backbone of each chain is formed by phosphodiester bonds between the 3' and 5' carbons of adjacent sugars. The two chains being held together by hydrogen bonds between the nitrogenous bases which point in towards the centre of the helix. The two DNA strands are held together by hydrogen bonds between the bases on each strands, forming base pairs (bp).
25 phosphodiester
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28 The arrangement of the bases in the DNA is not random: G in one chain always pairs with C in the other chain. A always pairs with T i.e. this base pairing forming complementary strands.
29 if DNA with 100pb G=60pb C=?, A=?, T=? STRUCTURE OF DNA 5 S 5 end 3 end P complementary base pairing T A G C 3 3 A T T A 5
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33 The DNA chain end terminated by the 5' carbon atom of the sugar molecule is referred to as the 5' end (5 prime), and the end terminated by the 3' carbon atom is called the 3' end (3 prime). In the DNA duplex the 5' end of one strand is opposite the 3' end of the other.
34 There are two different types of nucleic acid: 1- Ribonucleic acid (RNA) which contains five carbon sugar ribose. 2- Deoxyribonucleic acid (DNA) in which the hydroxyl group at the position 2 of the ribose sugar is replaced by a hydrogen, i.e. an oxygen molecule is lost.
35 SUGAR-PHOSPHATE BACKBONE Phosho-diester link between 3-OH of ribose and 5 phosphate of next nucleotide
36 BASE PAIRING Hydrogen bonds Adenine = Thymine (in DNA) Adenine = Uracil (in RNA) Cytosine =Guanine
37 BASE PAIRING DNA Base Pairs DNA-RNA base pairs A A One strand of DNA RNA
38 BASE PAIRING DNA Base Pairs DNA-RNA base pairs One strand of DNA RNA
39 DNA and RNA both contain A, G & C but T occurs only in DNA while U is only found in RNA. RNA is present in the cytoplasm and in particularly high concentrations in the nucleolus of the nucleus. DNA is found mainly in the chromosomes (nucleus and mitochondria).
40 COMPARISON OF DNA & RNA RNA DNA Sugar Ribose Deoxyribose Bases AUCG ATCG Strand length Short Long No. strands One Two Helix Single Double
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42 SUMMARY
translation The building blocks of proteins are? amino acids nitrogen containing bases like A, G, T, C, and U Complementary base pairing links
The actual process of assembling the proteins on the ribosome is called? translation The building blocks of proteins are? Complementary base pairing links Define and name the Purines amino acids nitrogen
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