Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 ccredited to Laboratory locations: Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK 22/4 Mill Road Industrial Estate Linlithgow ridge Scotland EH49 7SF Issue No: 029 Issue date: 07 pril 2017 Contact: Dr Jennifer Newton Tel: +44 (0) Fax: +44 (0) lab@expressmicrobiology.co.uk Website: s covered by the organisation and their relevant activities details ctivity code ddress 22/4 Mill Road Industrial Estate Linlithgow ridge Scotland EH49 7SF Local contact Dr Jennifer Newton Tel: +44 (0) Fax: +44 (0) lab@expressmicrobiology.co.uk Microbiological Testing Chemical Testing ddress 42 Mill Road Industrial Estate Linlithgow ridge Scotland EH49 7SF Local contact Dr Jennifer Newton Tel: +44 (0) Fax: +44 (0) lab@expressmicrobiology.co.uk Chemical Testing ssessment Manager: PC3 Page 1 of 8

2 Schedule of ccreditation United Kingdom ccreditation Service Issue No: 029 Issue date: 07 pril 2017 ccredited to DETIL OF CCREDITTION Chemical Tests FOOD and FOOD PRODUCTS Moisture In-house method using oven drying sh in-house method Sodium in-house method ashing followed by flame photometry Protein based on Dumas method Total Dietary Fibre based on OC Total sugars: Fructose, Galactose, Glucose Lactose, Maltose, Sucrose Fatty cid Composition - Saturates - Mono-unsaturates - Poly-unsaturates - Trans fatty acids - Omeg-3 acids - Omega-6 acids by ion chromatography by GC-FID Moisture Total Fat Calculated Values Calculation of salt content Calculation of aqueous salt Carbohydrate by Difference Energy Microwave drying and fat by Nuclear Magnetic Resonance using CEM Smart System Microwave by calculation from sodium (16.1.4) by calculation from sodium (16.1.4) ccording to Food Information Regulations 2014 ssessment Manager: PC3 Page 2 of 8

3 Schedule of ccreditation United Kingdom ccreditation Service Issue No: 029 Issue date: 07 pril 2017 ccredited to FOOD and FOOD PRODUCTS (cont d) Chemical Tests (cont d) Calculated Values (cont d) pparent Meat Content (Stubbs & Moore) pparent Meat Content (Separated) (Stubbs & Moore) dded Water (modified Stubbs & Moore) pparent Fish Content (Stubbs & Moore) pparent Fish Content (Separated) (Stubbs & Moore) Campden & Chorleywood Meat and Meat products: Calculations of Meat Content, dded Water and Connective tissue. Chemical Tests FOOD and FOOD Products and SWS Determination of Gluten using r-biopharm R5 ELIS Ridascreen kit. Fish, Fish Products and Cheese Determination of Histamine by ELIS using Histasure ELIS Fast Track NIML FEEDS and COMPOST / COMPOSTS Microbiological Tests Detection: Documented In-house Methods Salmonella spp. Method No17D based on S EN ISO 6579:2002+1:2007 in accordance with The nimal y- Products(Enforcement) (Scotland) Regulation 2015 and The nimal y-products (Enforcement) (England) Regulation 2015 and The nimal y-products (Enforcement) (Wales) Regulation ssessment Manager: PC3 Page 3 of 8

4 Schedule of ccreditation United Kingdom ccreditation Service Issue No: 029 Issue date: 07 pril 2017 ccredited to Microbiological Tests (cont d) Documented In-house Methods Detection (cont d): ENVIRONMENTL SMPLES oot/sock/overshoe swabs, surface swabs, cloacal swabs, bulked faeces, dust, chick papers/hatcher tray liners and DO organs Salmonella spp (including serotyping groups -G) Method No 17C based on S EN ISO 6579: :2007 in accordance with: Control of Salmonella in Poultry Order (CSPO), (England) 2007, (Wales) 2008 Control of Salmonella in Poultry (reeding, Laying and roiler Flocks) (Scotland) Order 2009 Control of Salmonella in Turkey Order (England) 2009, (Scotland) 2009 and (Wales) 2010 Control of roiler Flocks Order (England) 2009, (Wales) 2009 FOODS ND ENVIRONMENTL SMPLES Microbiological Tests Enumeration: based on standard methods as referenced acillus cereus, presumptive 1) Method No 28bf using PEM selective agar at 37 C for 24 hrs, and subsequently at room temperature for 24hrs. 2) Method No 28b using chromogenic medium CC at 37 C for 24hrs, and confirmation with gram stain, spore stain and haemolysis. Coliforms presumptive and confirmed Method No 26 based on ISO 4832:2006 ssessment Manager: PC3 Page 4 of 8

5 Schedule of ccreditation United Kingdom ccreditation Service Issue No: 029 Issue date: 07 pril 2017 ccredited to FOODS ND ENVIRONMENTL SMPLES (cont d) Microbiological Tests (cont d) Enumeration: (cont d) Clostridium perfringens, confirmed Method No 19 based on S EN ISO 7937:2004 Enterobacteriaceae, presumptive and confirmed Method No 27 based on S ISO 21528:Part 2:2004 Escherichia coli, ß- glucuronidase positive 1) Method No 23 based on ISO :2001 2) Method no 23C based on ISO :2001 using membranes Lactic cid acilli (presumptive) Method No 31 based on S EN ISO 15214:1998 Listeria spp and Listeria monocytogenes confirmed Method No 22MS based on S EN ISO Part 2:1998 1:2004 Pseudomonas spp, presumptive Method No 11 using spread plate on pseudomonas agar (CFC) based on ISO 13720:2010 Coagulase positive Staphylococci, confirmed Method No 16 based on S EN ISO:6888:Part 1:1999 using Staphytec plus latex confirmation Total erobic Colony Count at 30 C 1) Method No. 10 based on S EN ISO :2013 2) Method No. 10 using pour plate technique with Plate count agar at 30 C for 48hrs. Yeasts and Moulds (products with aw >0.95) Method No 20 based on S ISO :2008 ssessment Manager: PC3 Page 5 of 8

6 Schedule of ccreditation United Kingdom ccreditation Service Issue No: 029 Issue date: 07 pril 2017 ccredited to FOODS ND ENVIRONMENTL SMPLES (cont d) Microbiological Tests (cont d) Detection: Escherichia coli, confirmed Method No 23 based on S ISO 7251:2005 Escherichia coli O157, presumptive Method No 32 based on S EN ISO 16654:2001 (without Immunomagnetic separation) Listeria spp and Listeria monocytogenes 1) Method No 22MS based on S EN ISO Part 1:1997 1:2004 2) Method No 22FSPP using Half Fraser roth chromogenic medium (LO) Listeria spp Method No 22 using Oxoid ONE roth-listeria and rilliance Listeria agar (Oxoid Precis method) Salmonella spp, presumptive or confirmed Method No 17 based on S EN ISO 6579: :2007 Salmonella spp Method No 17 ELIS screening test using Solus Salmonella pre-enrichment with Method No 17 confirmatory tests FOOD Eggs only Salmonella spp. Method 17E documented in house method in accordance with ritish Egg Industry Council of Practice for Lion Eggs nnex Q 2013, Issue 1, based ISO 6579: :2007 FOOD Poultry only Campylobacter spp, confirmed Method No 34 based on ISO :2006 for testing poultry only ssessment Manager: PC3 Page 6 of 8

7 Schedule of ccreditation United Kingdom ccreditation Service Issue No: 029 Issue date: 07 pril 2017 ccredited to FOODS (raw and finished products) and ENVIRONMENTL SWS/SPONGES Molecular iology Tests Detection of: In-House Documented Methods Listeria spp DN Method No 22, Qualicon X PCR System Q7 Salmonella spp DN Method No 17, Qualicon X PCR System Q7, OC official method Shellfish, smoked fish, fish products Vibrio spp DN (Vibrio parahaemolyticus, Vibrio cholera, Vibrio vulnificus) Method No 33 by real-time PCR using Qualicon X PCR System Q7 WTERS, piped supplies, swimming pools, recreational waters Microbiological Tests Enumeration: based on the procedures in The Microbiology of Drinking Water (year identified in parenthesis) Total Colony Count at 22 C Method No 1 ( Part 7, 2012) Total Colony Count at 37 C Method No 1(Part 7, 2012) Coliforms, confirmed and presumptive E coli, confirmed and presumptive Pseudomonas aeruginosa, confirmed Enterococci, confirmed Sulphite Reducing Clostridia Method No 7, membrane filtration using MLG (Part 4, 2009) Method No 7, membrane filtration using MLG (Part 4, 2009) Method No 8, membrane filtration (Part 8, 2010) Method No 25w, membrane filtration (Part 5, 2012) Method No 24w, membrane filtration (Part 6, 2009) ssessment Manager: PC3 Page 7 of 8

8 Schedule of ccreditation United Kingdom ccreditation Service Issue No: 029 Issue date: 07 pril 2017 ccredited to WTERS, piped supplies, swimming pools, recreational waters and untreated (excluding seawater) Microbiological Tests (cont d) Detection and Enumeration: Coliforms based on standard methods as referenced Method No 36, using COLILERT (Part 4D 2009) E coli Method No 36, using COLILERT (Part 4D 2009) WTERS Potable, process, cooling towers Enumeration: Isolation and enumeration of Legionella pneumophila sero-groups 1 and 2-14 and Legionella spp Method 35 based on ISO 11731:1998 with serotyping and speciation by Microgen Latex kit END ssessment Manager: PC3 Page 8 of 8

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