(Spin Column) For purification of DNA and RNA from formalin-fixed, paraffin-embedded tissue sections. Instruction for Use
|
|
- Marylou Wilcox
- 6 years ago
- Views:
Transcription
1 AmoyDx FFPE DNA/RNA Kit (Spin Column) For purification of DNA and RNA from formalin-fixed, paraffin-embedded tissue sections Instruction for Use Instruction Version: B1.3 Revision Date: September 2015 Store kit dry at room temperature (15~25 ) 0 / 6
2 Introduction The AmoyDx FFPE DNA/RNA Kit provides silica-based membrane and special lysis buffer system for FFPE tissue DNA/RNA extraction effectively. This kit is specially designed for simultaneously isolation and purification of DNA and RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The purified DNA/RNA is suitable for downstream applications such as reverse transcription, RT-PCR, and real-time quantitative RT-PCR (qrt-pcr). Principle FFPE specimen tissue sections are first deparaffinized with xylene/ethanol method, then incubated in Buffer RTL and Proteinase K solution, to release DNA and RNA from the sections. After centrifugation RNA is in the supernatant, and DNA is in underlying the precipitates. DNA Extraction: The precipitates incubated in Buffer DTL and Proteinase K solution to completely release DNA. A short incubation in Buffer DES at a higher temperature partially reverses formalin crosslinking of the released nucleic acids, improving DNA yield and quality as well as DNA performance in downstream assays. The lysate is mixed with Buffer DTB and ethanol to provide appropriate binding conditions for DNA, then the mixture is applied to a DNA spin column, where the DNA binds to the membrane and impurities are removed with wash buffer. The DNA is eluted in Buffer DTE. RNA Extraction: The supernatant incubated at 80 to partially reverse formalin crosslinking of the released nucleic acids, improving RNA yield and quality as well as RNA performance in downstream enzymatic assays. Next, genomic DNA in the solution is removed with the DNase I. The lysate is mixed with Buffer RTB and ethanol to provide appropriate binding conditions for RNA, and the sample is then applied to a RNA spin column, where the total RNA binds to the membrane and impurities are removed with wash buffer. The total RNA is eluted in buffer RTE. Kit Contents This kit contains sufficient reagents to perform 36 tests (Table 1). Table 1 Kit Contents Item Amount Tube No. DNA Spin Columns 36 RNA Spin Columns 36 Collection Tubes (2 ml) 2 72 Centrifugal Tubes (1.5 ml) 2 72 Buffer RTL 10 ml 1 Proteinase K Solution 1.8 ml 2 DNase I Magic Buffer 1.5 ml 3 DNase I (30 U/µL) 40 µl 4 Buffer RTB 15 ml 5 Buffer RTW (concentrate) 2 7 ml 6 Buffer RTE 8 ml 7 RNase-free Water 1.5 ml 8 Buffer DTL 10 ml 1 1 / 6
3 Note: Buffer DES 900 µl 2 Buffer DTB 10 ml 3 Buffer DW1 (concentrate) 13 ml 4 Buffer DW2 (concentrate) 6 ml 5 Buffer DTE 8 ml 6 Instruction for Use 1 1. Buffer RTB, Buffer DTB and Buffer DW1 contain guanidine salt, not compatible with disinfectants containing bleach or acidic solutions. 2. For the first time use, add 21 ml ethanol (96~100%) into Buffer RTW (concentrate), add 17 ml ethanol (96~100%) into Buffer DW1 (concentrate) and mix thoroughly; add 24 ml ethanol (96~100%) into Buffer DW2 (concentrate ) and mix thoroughly. Tick the check box on the bottle label. 3. Store the DNase I (30U/µL) at -20±5 upon receipt will be helpful for keeping the activity. For the first time use, add 360 µl RNase-free Water into DNase I (30 U/µL) to obtain 3 U/µL solution, mix well by pipetting gently up and down. Equipment and Reagents Not Supplied With Kits 1. Sterile, RNase-free pipet tips 2. Ethanol (96~100%) 3. Xylene 4. RNaseA (optional) 5. Water bath or heated orbital incubator (37~90 adjustable) 6. Microcentrifuge (10000~13000 g adjustable) 7. Vortexer 8. Palm centrifuge 9. Recommend: microtome suitable for sectioning paraffin-embedded tissue that is capable of producing 5~10 μm sections. Storage and Expiry Date The shelf life of the kit is 12 months. The kit should be stored dry at room temperature (15~25 ). Once opened, this reagent is stable at room temperature until the expiry. Starting material Standard formalin-fixation and paraffin-embedding procedures always result in significant fragmentation of nucleic acids. To limit the extent of DNA/RNA fragmentation, be sure to: 1. Fix tissue samples in 4~10% neutral formalin solution as quickly as possible after surgical removal. 2. Use a fixation time of 14~24 hours (longer fixation time leads to more severe DNA/RNA fragmentation, resulting in poor performance in downstream assays). 3. Thoroughly dehydrate samples prior to embedding (residual formalin maybe inhibit the digestion of the Proteinase K). 4. The starting material for DNA/RNA purification should be freshly cut sections of FFPE tissue, each with a thickness of up to 5~10 μm. (Thicker sections may result in lower DNA/RNA yields, even after prolonged incubation with proteinase K). 2 / 6
4 5. The FFPE tissue area should be 0.5~1 cm 2. If the FFPE tissue surface area is less than 0.5 cm 2, please use more sections. 6. FFPE sample s storage time should be less than three years. Guidelines for Sectioning Paraffin Blocks To use this kit, it needs 5~10 μm sections of the tissue in paraffin block. You may use any method for sectioning the paraffin blocks. General guidelines for sectioning paraffin blocks are outlined below: 1. Avoid nuclease contamination by using a clean, sharp microtome blade and tweezers. 2. When multiple samples are processed, clean the microtome blade and tweezers with RNase-inactivating agents to avoid cross-contamination of nucleic acids and RNases. UV irradiation for 10 minutes is recommended after cleaning. 3. Always wear latex or nitrile gloves. 4. Cut 5~10 μm thick sections from trimmed paraffin blocks with a tissue surface area about 0.5~1 cm 2. General Handling of RNA Observe the following guidelines to prevent RNase contamination and maximize the RNA yield: 1. Use disposable, sterile plastic ware. 2. Use sterile, new pipette tips and microcentrifuge tubes. 3. Wear latex or nitrile gloves while handling reagents and RNA samples to prevent RNase contamination from the surface of the skin. 4. Use proper microbiological aseptic technique when working with RNA. 5. Use proper method to remove RNase contamination from surfaces. Safety Information Follow the safety guidelines below when using the kit. 1. Treat all reagents supplied in the kit as potential irritants. 2. Wear a lab coat, disposable gloves, and protective goggles. 3. If a buffer spill occurs, clean with a suitable laboratory detergent and water. 4. Dispose any tissue in designated biohazard containers. 5. If a spill contains potentially infectious reagents, clean the affected area first with laboratory detergent and Protocol water, then with 1% (v/v) sodium hypochlorite or a suitable laboratory disinfectant. 1. Deparaffinization 1.1 Using a scalpel, trim excess paraffin off the sample block. 1.2 Cut sections with a thickness of 5~10 μm and a surface area between 0.5~1 cm Immediately place 2~5 sections in a 1.5 ml centrifugal tube. 1.4 Add 1 ml xylene, close the lid and vortex vigorously for 10 seconds. 1.5 Centrifuge at g for 2 min at room temperature. 1.6 Remove the supernatant by pipetting (do not remove any of the pellet). 1.7 Add 1mL ethanol (96~100%) to the pellet, and mix by vortexing for 10 seconds. 1.8 Centrifuge at g for 2 min at room temperature. 1.9 Remove the supernatant by pipetting (do not remove any of the pellet) Open the tube and incubate at room temperature for 10 min, or at 37 for 5 min. Make sure all residual ethanol has evaporated before proceeding. 3 / 6
5 2. Tissue Lysis 2.1 Add 200 µl Buffer RTL and 25 µl Proteinase K Solution into the tube above, mix gently by pipetting up and down. 2.2 Incubate at 56 for 15 min. 2.3 Centrifuge at g for 2 min. 2.4 Transfer 180 µl supernatant to a clean 1.5 ml centrifugal tube for RNA extraction. 2.5 The remaining solution and precipitates are used for DNA extraction. 3. DNA Extraction For the first time use, please add 17 ml ethanol (96~100%) into Buffer DW1 (concentrate), add 24 ml ethanol (96~100%) into Buffer DW2 (concentrate), and mark it clearly. Before the DNA extraction, please check the reagents without leakage. Shake the reagents gently to mix the solutions. If the reagents contain precipitates, dissolved by heating at Add 140 µl Buffer DTL and 15 µl Proteinase K Solution into the underlying precipitates, mix gently by pipetting up and down. 3.2 Incubate at 56 for 1 hour for lyse the sample tissue. If the tissue has not been completely lysed, or need higher concentration of DNA, incubate for further time or overnight. 3.3 Add 10 µl Buffer DES, mix gently by vortexing and briefly centrifuge for 5~10 seconds. Transfer the centrifugal tube to heated orbital incubator and incubate at 90 for 2 hour. 3.4 Briefly centrifuge for 5~10 seconds. If RNA-free genomic DNA is required, allow the sample to cool to room temperature, add 2 µl RNase A (100 mg/ml) and incubate for 5 min at room temperature. 3.5 Add 200 µl Buffer DTB and 200 µl ethanol (96~100%), mix by vortexing. 3.6 Briefly centrifuge for 5~10 seconds. 3.7 Transfer the entire lysate to the DNA Spin Column (in a 2 ml collection tube) without wetting the rim, close the lid, and centrifuge at g for 1 min. 3.8 Discard the flow-through in collection tube. 3.9 Add 600 µl Buffer DW1 to DNA Spin Column, centrifuge at g for 1 min Discard the flow-through in collection tube Add 600 µl Buffer DW2 to DNA Spin Column, centrifuge at g for 1 min Discard the collection tube with flow-through Place the DNA Spin Column in a clean 2 ml collection tube, centrifuge at g for 3 min Discard the collection tube with flow-through Place the DNA Spin Column in a clean 1.5 ml centrifugal tube Apply 30~100 µl Buffer DTE to the center of the membrane. Do not touch the membrane Incubate at room temperature (15~25 ) for 2~5 min Centrifuge at g for 1 min The eluted DNA is immediately ready for use or for storage under -20. Note: Decrease the volume of Buffer DTE properly to obtain a higher concentration of DNA. 4. RNA Extraction For the first time use, please add 21 ml ethanol (96~100%) into Buffer RTW (concentrate), and mark it clearly. For the first time use, please add 360 µl RNase-free Water into DNase I (30 U/µL) to obtain 3 U/µL solution, and mix well by pipetting gently up and down. DNase I working solution should be stored at 4 / 6
6 -20±5. Before the RNA extraction, please check the reagents without leakage; shake the reagents gently to mix the solution. If the reagents contain a precipitate, dissolved by heating at Transfer the centrifugal tube which contains 180 µl supernatant to heated orbital incubator and incubate at 80 for 15 min. 4.2 Allow the sample to cool to room temperature, then briefly centrifuge for 5~10 seconds. 4.3 According to the ratio of 20 µl DNase I Magic Buffer and 10 µl DNase I (3 U/µL) per sample, mix DNase I Magic Buffer and DNase I (3 U/µL) by pipetting up and down to get DNase I working solution. 4.4 Add 30 µl DNase I working solution to the sample, mix gently by pipetting up and down. 4.5 Stand at room temperature for 15 min. 4.6 Add 340 µl Buffer RTB and 750 µl ethanol (96~100%), mix thoroughly by pipetting gently up and down. 4.7 Briefly centrifuge for 5~10 seconds. 4.8 Transfer 650 µl lysate to the RNA Spin Column (in a 2 ml collection tube) without wetting the rim, close the lid, and centrifuge at g for 30 seconds. 4.9 Discard the flow-through in collection tube Transfer the remaining solution to the RNA Spin Column, without wetting the rim, close the lid Centrifuge at g for 30 second Discard the flow-through in collection tube Add 600 µl Buffer RTW to RNA Spin Column, centrifuge at g for 30 seconds Discard the flow-through in collection tube Add 600µL Buffer RTW to RNA Spin Column, centrifuge at g for 30 seconds Discard collection tube with flow-through Place the RNA Spin Column in a clean 2 ml collection tube, centrifuge at g for 5 min Discard the collection tube with flow-through Place the RNA Spin Column in a clean 1.5 ml centrifugal tube Apply 30~80 µl Buffer RTE to the center of the membrane. Do not touch the membrane Stand at room temperature (15~25 ) for 1~3 min Centrifuge at g for 1 min The eluted RNA is ready for use. If it s not used immediately, it should be stored under -70. Note: Decrease the volume of Buffer RTE properly to obtain a higher concentration of RNA. Warnings and Precautions 1. Please read the instruction carefully and become familiar with all components of the kit prior to use. 2. The extracted DNA and RNA quality is subject to the influence of such factors as sample source, sampling process, formalin fixation, paraffin embedding and storage conditions. 3. Sample quality has a high impact on quality and amount of the purified DNA and RNA. 4. Due to fixation and embedding conditions, nucleic acids in FFPE samples are usually heavily fragmented and chemically modified by formaldehyde. DNA/RNA purified from FFPE samples should not be used in downstream applications that require full-length DNA/RNA. 5. Buffer RTB, Buffer DTB and Buffer DW1 contain guanidine salt, which can form highly reactive 5 / 6
7 compounds when combined with bleach. Do not add bleach or acidic solutions directly to the sample-preparation waste. If the liquid containing this buffer is spilt, clean with suitable laboratory detergent and water. 6. Unless otherwise indicated, perform all steps of the procedure at room temperature (15~25 ). 7. For the first time use, make sure that Buffer RTW (concentrate), Buffer DW1 (concentrate) and Buffer DW2 (concentrate) are diluted with the ethanol (96~100%) as described and mixed well. 8. Do not exchange and mix up the kit contents with different batches. 9. We recommend the use of aerosol-barrier filter pipet tips; avoid touching the membrane with the pipet tips. Open only one tube at a time, and take care to avoid generating aerosols. 10. Only trained professionals should use this kit. Please wear suitable lab coat and disposable gloves. 11. The used kit and flow-through fractions may contain hazardous waste and should be disposed of properly. Notes 1. Symbol for "AUTHORISED REPRESENTATIVE IN THE EUROPEAN COMMUNITY" 2. Symbol for "IN VITRO DIAGNOSTIC MEDICAL DEVICE" 3. Symbol for "KEEP DRY" 4. Symbol for "THIS WAY UP" 5. Symbol for "FRAGILE,HANDLE WITH CARE" Information of European Authorised Representative Wellkang Ltd t/a Wellkang Tech Consulting Suite B, 29 Harley Street, London W1G 9QR United Kingdom 6 / 6
QIAamp DNA FFPE Tissue Handbook
April 2010 QIAamp DNA FFPE Tissue Handbook For purification of genomic DNA from formalin-fixed, paraffin-embedded tissues Sample & Assay Technologies Trademarks: QIAGEN, QIAamp, MinElute, Qproteome, REPLI-g,
More informationReliaPrep FFPE gdna Miniprep System
TECHNICAL MANUAL ReliaPrep FFPE gdna Miniprep System Instructions for Use of Products A2351 and A2352 Revised 12/15 TM352 ReliaPrep FFPE gdna Miniprep System All technical literature is available at: www.promega.com/protocols/
More informationQIAamp DSP DNA FFPE Tissue Kit Handbook
February 2017 QIAamp DSP DNA FFPE Tissue Kit Handbook Version 1 50 For in vitro diagnostic use 60404 QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, GERMANY R3 1062689EN Sample to Insight QIAamp DSP DNA FFPE
More informationcelldatasci.com/rnastorm RNAstorm RNA Isolation Kit for FFPE Tissue Samples Sample Kit (20 extractions)
celldatasci.com/rnastorm info@celldatasci.com RNAstorm RNA Isolation Kit for FFPE Tissue Samples Sample Kit (20 extractions) Support: Email: support@celldatasci.com Phone: 650.285.2376 (option 2) Toll-free:
More informationFor simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues
1. TIANamp DNA/RNA Isolation Kit For simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues www.tiangen.com/en RP090603 TIANamp DNA/RNA Isolation Kit Kit Contents
More informationProduct # Specifications. Kit Specifications Maximum Column Binding Capacity (gdna) Maximum Column Loading Volume.
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com FFPE DNA Purification Kit Product # 47400 Product Insert Norgen
More informationEZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK
EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 8 Rev 05/0/03 EZ-0 Genomic DNA Kit Handbook Table of Contents Introduction Limitations of Use Features Applications
More informationE.Z.N.A. mirna Kit. R preps R preps R preps
E.Z.N.A. mirna Kit R7034-00 5 preps R7034-01 50 preps R7034-02 200 preps August 2011 E.Z.N.A. Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
More informationTIANamp Marine Animals DNA Kit
TIANamp Marine Animals DNA Kit For isolation of genomic DNA from marine animal tissues www.tiangen.com/en DP121221 TIANamp Marine Animals DNA Kit (Spin Column) Cat. no. DP324 Kit Contents Contents DP324-02
More informationGenUP Virus DNA/RNA Kit
Product Insert GenUP Virus DNA/RNA Kit LOT: See product label EXPIRY DATE: See product label ORDERING INFORMATION PRODUCT GenUP Virus DNA/RNA Kit CAT. NO. BR0701101 BR0701102 BR0701103 SIZE 10 preps 50
More informationNote: for laboratory research use only. RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Signalway Biotechnology
Note: for laboratory research use only RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Cat. #: RP1202 (50preps) Signalway Biotechnology I. Kit Content, Storage Condition and Stability Content
More informationCATS RNA-seq v2 library preparation protocol on RNA from FFPE-samples
CATS RNA-seq v2 library preparation protocol on RNA from FFPE-samples INTRODUCTION The following protocol offers a streamlined solution for whole transcriptome sequencing studies from human/ mouse/rat
More informationISOLATE II Blood DNA Kit. Product Manual
ISOLATE II Blood DNA Kit Product Manual 2 Product Manual www.bioline.com/isolate Blood DNA Kit ISOLATE II Blood DNA Kit ISOLATE II Blood DNA Kit 1 Kit contents 04 2 Description 04 3 Storage 05 4 Safety
More informationBlood DNA Extraction Kit
H A N D B O O K Blood DNA Extraction Kit NP-BD-050 050 Preps www.genetixbiotech.com Contents Page No COMPONENTS Kit Contents Reagents, Consumables and Equipment not provided with the kit SAFETY INSTRUCTIONS
More informationUrine DNA Isolation Maxi Kit (Slurry Format) Product # 50100
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation Maxi Kit (Slurry Format) Product # 50100 Product
More informationEZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK
EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 5.0 Rev 03/25/205 Table of Contents Introduction 2 Limitations of Use 2 Features 2 Applications 2 Storage 2
More informationPurification of cytoplasmic RNA from animal cells using the RNeasy Mini Kit
QIAGEN Supplementary Protocol: Purification of cytoplasmic RNA from animal cells using the RNeasy Mini Kit This protocol requires the RNeasy Mini Kit. IMPORTANT: Please consult the Safety Information and
More informationTIANamp Yeast DNA Kit
TIANamp Yeast DNA Kit For isolation of genomic DNA from yeast cells www.tiangen.com/en DP121221 TIANamp Yeast DNA Kit Kit Contents (Spin Column) Cat. no. DP307 Contents Buffer GA Buffer GB Buffer GD Buffer
More informationFor Research Use Only Ver
INSTRUCTION MANUAL ZR-Duet DNA/RNA MiniPrep Plus Catalog No. D7003 Highlights Efficient isolation and separation of DNA and RNA from any cells, tissue, blood, and biological fluids. High quality DNA and
More informationQIAamp Fast DNA Stool Mini Handbook
March 2014 QIAamp Fast DNA Stool Mini Handbook For fast purification of genomic DNA from stool samples Sample & Assay Technologies QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of
More informationPreAnalytiX Supplementary Protocol
PreAnalytiX Supplementary Protocol Purification of total RNA from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues This protocol is for
More informationCells and Tissue DNA Isolation Kit (Magnetic Bead System) 50 Preps Product # 59100
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cells and Tissue DNA Isolation Kit (Magnetic Bead System) 50 Preps
More informationKit Components Product # (50 samples) Wash Solution A Elution Buffer B
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cells and Tissue DNA Isolation Kit Product # 53100 Product Insert
More informationE.Z.N.A. Stool DNA Kit. D preps D preps D preps
E.Z.N.A. Stool DNA Kit D4015-00 5 preps D4015-01 50 preps D4015-02 200 preps April 2013 E.Z.N.A. Stool DNA Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-RNA Whole Blood Catalog Nos. R1201 Highlights Purify high-quality total RNA (including small/micro RNAs) from whole and partitioned blood samples. Compatible with commonly used
More informationE.Z.N.A. Tissue RNA Kit. R preps R preps
E.Z.N.A. Tissue RNA Kit R6688-00 5 preps R6688-01 50 preps May 2015 E.Z.N.A. Tissue RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Important Notes...4 Homogenization
More informationE.Z.N.A. PF Micro RNA Kit. R preps R preps
E.Z.N.A. PF Micro RNA Kit R7036-00 5 preps R7036-01 50 preps June 2013 E.Z.N.A. PF Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4
More informationPlasmid Midiprep Plus Purification Kit. Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only
Plasmid Midiprep Plus Purification Kit Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only 1 Description: The Plasmid Midiprep Plus Purification Kit provides simple
More informationE.Z.N.A. Stool DNA Kit. D preps D preps D preps
E.Z.N.A. Stool DNA Kit D4015-00 5 preps D4015-01 50 preps D4015-02 200 preps July 2017 E.Z.N.A. Stool DNA Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage
More informationPresto Mini RNA Bacteria Kit
Instruction Manual Ver. 03.22.17 For Research Use Only Presto Mini RNA Bacteria Kit RBB004, RBBD004 (4 Preparation Sample Kit) RBB050, RBBD050 (50 Preparation Kit) RBB100, RBBD100 (100 Preparation Kit)
More informationLysis Buffer A Wash Solution A Elution Buffer B Mini Filter Spin Columns 50 Collection Tubes 50 Elution tubes (1.7 ml) 100 Product Insert 1
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation Kit (Slurry Format) Product # 48800 Product
More informationE.Z.N.A. Blood DNA Mini Kit. D preps D preps D preps
E.Z.N.A. Blood DNA Mini Kit D3392-00 5 preps D3392-01 50 preps D3392-02 200 preps January 2017 E.Z.N.A. Blood DNA Mini Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage
More informationProtocol for DNA extraction from FFPE Samples
25, ave Georges Lemaître - B-6041 Gosselies (Belgique) Tel : +32 (0)71 37 85 27 - Fax : +32 (0)71 34 78 79 Protocol for DNA extraction from FFPE Samples Step 1. Paraffin Removal 1 Equilibrate a heat block
More informationPowerSoil DNA Isolation Kit
PowerSoil DNA Isolation Kit Catalog No. Quantity 12888-50 50 Preps 12888-100 100 Preps Instruction Manual Introduction The PowerSoil DNA Isolation Kit* is comprised of a novel and proprietary method for
More informationMD60002 MD MD62002
MagSi-DNA saliva Art.No. MD60002 MD61002 - MD62002 Product Manual Version 1.0 22/01/2015 Table of Contents 1. General Information...3 1.1 Intended Use...3 1.2 Kit specifications...3 1.3 Basic principle...3
More informationKit Specifications 45 g. 45 g of RNA 8 L
RNA Clean-Up and Concentration Micro-Elute Kit Product # 61000 Product Insert Norgen s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration
More informationSpecifications. Kit Specifications. Alcohol Precipitation: Up to 100 ml Column Purification: Up to 5 ml Column Binding Capacity 25 µg
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com BAC DNA MiniPrep Kit Product # 18050 Product Insert The BAC DNA
More informationE.Z.N.A. Water DNA Kit. D preps D preps D preps
E.Z.N.A. Water DNA Kit D5525-00 5 preps D5525-01 50 preps D5525-02 200 preps April 2017 E.Z.N.A. Water DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
More informationPresto Mini gdna Bacteria Kit
Instruction Manual Ver. 02.10.17 For Research Use Only Presto Mini gdna Bacteria Kit Advantages GBB004 (4 Preparation Sample Kit) GBB100/101 (100 Preparation Kit) GBB300/301 (300 Preparation Kit) Sample:
More informationAverage Yields* Yeast DNA Yeast RNA Time to Complete 10 Purifications * Yield will vary depending on the type of sample processed
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Fungi/Yeast RNA/DNA Purification Kit Product # 35800 Product Insert
More informationRNA Clean-Up and Concentration Kit Product # 23600, 43200
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com RNA Clean-Up and Concentration Kit Product # 23600, 43200 Product
More informationUltraClean Midi Plasmid Prep Kit
UltraClean Midi Plasmid Prep Kit Catalog No. Quantity 12700-20 20 Preps Instruction Manual Please recycle Version: 05232014 1 Table of Contents Introduction... 3 Protocol Overview... 3 Flow Chart... 4
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-RNA Miniprep Plus Kit Catalog Nos. R1057T, R1057, & R1058 Highlights Purify high-quality total RNA (including small/micro RNAs) from any cells, tissues, blood and biological fluids.
More informationProduct # 24700, 24750
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Genomic DNA Isolation Kit Product # 24700, 24750 Product Insert
More informationPlant/Fungi Total RNA Purification Kit Product # 25800, 31350, 25850
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plant/Fungi Total RNA Purification Kit Product # 25800, 31350,
More informationPlease read all the information in booklet before using the unit
100 Please read all the information in booklet before using the unit Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email:
More informationSize-Select Kit for NGS Library Preparation Product # 53600
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Size-Select Kit for NGS Library Preparation Product # 53600 Product
More informationE.Z.N.A. MicroElute Genomic DNA Kit. D preps D preps D preps
E.Z.N.A. MicroElute Genomic DNA Kit D3096-00 5 preps D3096-01 50 preps D3096-02 200 preps December 2013 E.Z.N.A. MicroElute Genomic DNA Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3
More informationE.Z.N.A. Bacterial RNA Kit. R preps R preps
E.Z.N.A. Bacterial RNA Kit R6950-00 5 preps R6950-01 50 preps July 2017 E.Z.N.A. Bacterial RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Before Beginning...4
More informationULTRAPrep PLASMID DNA KIT
ULTRAPrep RNA Tissue Total RNA isolation from cultured human and animal cells ULTRAPrep RNA Cell Culture Total RNA isolation from cultured human and animal cells 2 2 6-024-01-0 6-024-02-0 6-021-01-0 6-021-02-0
More informationMag-Bind Universal Pathogen 96 Kit. M x 96 preps M x 96 preps
M4029-00 1 x 96 preps M4029-01 4 x 96 preps August 2015 Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4 Tissue Protocol...5 Serum/Stool Protocol...9
More informationPlasma/Serum RNA/DNA Purification Mini Kit Product# 55200
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plasma/Serum RNA/DNA Purification Mini Kit Product# 55200 Product
More informationSupplementary File 3: DNA and RNA isolation
Supplementary File 3: DNA and RNA isolation Q-CROC-02 Biopsy protocol For the purposes of this protocol, four needle core biopsies (NCBs) of lymph node tissue are isolated from each patient using a 16G
More informationManual innuprep Micro RNA Kit
Manual Order No.: 845-KS-2030010 10 reactions 845-KS-2030050 50 reactions 845-KS-2030250 250 reactions Publication No.: HB_KS-2030_e_120116 This documentation describes the state at the time of publishing.
More informationCytoplasmic & Nuclear RNA Purification Kit Product # 21000, 37400
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cytoplasmic & Nuclear RNA Purification Kit Product # 21000, 37400
More informationIntroduction. Principle
Contents Introduction............................................................ 2 Principle.............................................................. 2 Storage and Stability.....................................................
More informationContents. Introduction Methods Appendix Kit Contents and Storage...iv. System Overview... 1 Experimental Outline...
Genomed User Manual ii Contents Kit Contents and Storage...iv Introduction... 1 System Overview... 1 Experimental Outline... 3 Methods... 4 General Information... 4 Purifying gdna from Blood and Body Fluids
More informationFastGene Plasmid Mini Kit For isolation of high copy and low copy plasmid DNA
FastGene Plasmid Mini Kit For isolation of high copy and low copy plasmid DNA Cat.No.: FG-90402 for 100 preparations Cat.No.: FG-90502 for 300 preparations STORAGE Store at room temperature (15-25 C) FastGene
More informationPinpoint Slide DNA Isolation System Catalog No. D3001
INSTRUCTIONS Pinpoint Slide DNA Isolation System Catalog No. D3001 Highlights Easily isolates genomic DNA in any targeted microscopic tissue area on a slide. The simple procedure combines Pinpoint tissue
More informationsbeadex maxi plant kit
sbeadex maxi plant kit Catalogue numbers 41602 and 41620 (For research use only. Not for use in diagnostic procedures.) Contents 1. Introduction 2. Kit contents and storage conditions 2.1 sbeadex maxi
More informationFosmidMAX DNA Purification Kit
Cat. No. FMAX046 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 204 10/2012 1 EPILIT204 Rev. A
More informationAmoyDx PIK3CA Five Mutations Detection Kit
AmoyDx PIK3CA Five Mutations Detection Kit Detection of five mutations in PIK3CA gene Instruction for Use Instruction Version: B2.2 Revision Date: June 2016 Store at -20±5 Background For: ADx-PI01 Phosphatidylinositol
More informationUltraClean Endotoxin-Free Mini Plasmid Prep Kit
UltraClean Endotoxin-Free Mini Plasmid Prep Kit Catalog No. Quantity 12311-100 100 Preps Instruction Manual Please recycle Version: 11172015 1 Table of Contents Introduction... 3 Protocol Overview... 3
More informationManual innuprep Virus DNA Kit
Manual Order No.: 845-- reactions 845-- reactions 845-- reactions Publication No.: HB_KS-_e_1 This documentation describes the state at the time of publishing. It needs not necessarily agree with future
More informationHighPrep Blood & Tissue DNA Kit
MAGBIO ACCELERATING genomic research HighPrep Blood & Tissue DNA Kit Manual Revision v1.1 Catalog Nos. HPBTS-D16, HPBTS-D96, HPBTS-D96X4 Genomic DNA isolation from 20-250 μl of blood, lysate of tissues,
More informationBiOstic FFPE Tissue RNA Isolation Kit (For use with formalin-fixed and paraffin-embedded tissues)
BiOstic FFPE Tissue RNA Isolation Kit (For use with formalin-fixed and paraffin-embedded tissues) Catalog No. Quantity 13250-50 50 preps Instruction Manual Please recycle Version: 02032014 1 Table of Contents
More informationFFPE DNA Extraction kit
FFPE DNA Extraction kit Instruction Manual FFPE DNA Extraction kit Cat. No. C20000030, Format 50 rxns Version 1 / 14.08.13 Content Introduction 4 Overview of FFPE DNA extraction workflow 4 Kit contents
More informationfoodproof Sample Preparation Kit III
For food testing purposes FOR IN VITRO USE ONLY Version 1, June 2015 For isolation of DNA from raw material and food products of plant and animal origin for PCR analysis Order No. S 400 06.1 Kit for 50
More informationE.Z.N.A. Blood DNA Maxi Kit. D preps D preps
E.Z.N.A. Blood DNA Maxi Kit D2492-00 2 preps D2492-03 50 preps April 2014 E.Z.N.A. Blood DNA Maxi Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4
More informationGenomic Mini Versatile kit for genomic DNA purification from various sources. version 0517
Genomic Mini Versatile kit for genomic DNA purification from various sources. version 0517 50 isolations, 250 isolations Cat. # 116-50, 116-250 Kit for DNA isolations from z tissues, bacteria, cell culture.
More informationfoodproof Magnetic Preparation Kit III
For food testing purposes FOR IN VITRO USE ONLY foodproof Magnetic Preparation Kit III Version 3, July 2015 For the automated isolation of DNA from raw material and food products of plant and animal origin
More informationHiYield TM Genomic DNA Extraction Kit
HiYield TM Genomic DNA Extraction Kit CONTENTS Genomic DNA Extraction Kit...... 1 Blood/Bacteria/Cultured Cells Cat.No. YGB50 // YGB100 // YGBM25 Blood Protocol..... 4 Cultured Cells Protocol...... 5 Bacterial
More informationBACMAX DNA Purification Kit
Cat. No. BMAX044 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 212 10/2012 1 EPILIT212 Rev. A
More informationManual innuprep Virus DNA/RNA Kit
Manual Order No.: 845-- reactions 845-- reactions 845-- reactions Publication No.: HB_KS-_e_1 This documentation describes the state at the time of publishing. It needs not necessarily agree with future
More informationQIAGEN GeneRead QIAact Panel Cleanup Kit Handbook
QIAGEN GeneRead QIAact Panel Cleanup Kit Handbook June 2017 For cleanup of DNA during construction of targeted, molecularly bar-coded libraries for digital sequencing with next-generation sequencing (NGS)
More informationCatalog No. SA-40002: 50 preparation SA-40001: 100 preparation
BDtract TM Genomic DNA Isolation Kit Instruction Manual Catalog No. SA-40002: 50 preparation SA-40001: 100 preparation Maxim Biotech, Inc. 780 Dubuque Avenue, So. San Francisco, CA 94080, U.S.A. Tel: (800)
More informationMMLV Reverse Transcriptase 1st-Strand cdna Synthesis Kit
MMLV Reverse Transcriptase 1st-Strand cdna Synthesis Kit Cat. No. MM070150 Available exclusively thru Lucigen. lucigen.com/epibio www.lucigen.com MA265E MMLV Reverse Transcriptase 1st-Strand cdna Synthesis
More informationPowerMax Soil DNA Isolation Kit
PowerMax Soil DNA Isolation Kit Catalog No. Quantity 12988-10 10 Preps Instruction Manual Inhibitor Removal Technology (IRT) is a registered trademark of MO BIO Laboratories, Inc. and is covered by the
More informationAbsolutely RNA Microprep Kit
Absolutely RNA Microprep Kit INSTRUCTION MANUAL Catalog #400805 Revision A For In Vitro Use Only 400805-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this product. No
More informationEZ-DNA. Instructions for Use. Genomic DNA Isolation Reagent. Product Description. Kit Reagent. Reagent Required But Not Supplied.
EZ-DNA Genomic DNA Isolation Reagent Cat. No.: 20-600-50 Store at: Room Temperature Instructions for Use Protocol for Genomic DNA Isolation Tissue Specific Recommendations for the Use of EZ-DNA Assessing
More informationTECHNICAL BULLETIN. GenElute mrna Miniprep Kit. Catalog MRN 10 MRN 70
GenElute mrna Miniprep Kit Catalog Numbers MRN 10, MRN 70 TECHNICAL BULLETIN Product Description The GenElute mrna Miniprep Kit provides a simple and convenient way to purify polyadenylated mrna from previously
More informationProduct # Specifications. Kit Specifications Maximum DNA Input. 200 g Maximum DNA Volume Input Final Endotoxin Levels
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Endotoxin Removal Kit (Midi) Product # 52200 Product Insert Norgen
More informationPureLink RNA Micro Kit
PureLink RNA Micro Kit For purification of total RNA from variety of samples in low elution volume Catalog no. 12183-016 Version B 19 December 2008 100003660 User Manual Table of Contents Kit Contents
More informationProduct # Kit Specification. Kit Specifications Maximum Column Binding Capacity 50 µg Maximum Column Loading Volume 650 µl
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Fungi/Yeast Genomic DNA Isolation Kit Product # 27300 Product
More informationUrine DNA Isolation 96-Well Kit (Slurry Format) Product # 27100
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation 96-Well Kit (Slurry Format) Product # 27100
More informationGeneaid DNA Isolation Kit (Yeast)
Geneaid DNA Isolation Kit (Yeast) GEY100, GEY300 Advantages Sample: up to 2 10 8 yeast and other fungus species Yield: high yield, high quality DNA (A260/A280 = 1.8-2.0) Format: scalable DNA precipitation
More informationPreAnalytiX Supplementary Protocol
PreAnalytiX Supplementary Protocol Purification of full-length proteins from sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue This protocol describes using the Qproteome FFPE Tissue kit
More informationE.Z.N.A. Total RNA Kit II. R preps R preps R preps
E.Z.N.A. Total RNA Kit II R6934-00 5 preps R6934-01 50 preps R6934-02 200 preps September 2015 E.Z.N.A. Total RNA Kit II Table of Contents Introduction...2 Illustrated Protocol...3 Kit Contents/Storage
More informationFast Ion. Plasmid Midi Advanced Kit (incl. Thimble) Plasmid Midi Advanced Kit. Protocol Book. Ion Exchange-High Yield, High Purity, Transfection-grade
Fast Ion Plasmid Midi Advanced Kit (incl. Thimble) Ion Exchange-High Yield, High Purity, Transfection-grade Plasmid Midi Advanced Kit Ion Exchange-High Yield, High Purity, Transfection-grade Protocol Book
More informationReliaPrep gdna Tissue Miniprep System
TECHNICAL MANUAL ReliaPrep gdna Tissue Miniprep System Instruc ons for Use of Products A2050, A2051 and A2052 Revised 8/13 TM345 ReliaPrep gdna Tissue Miniprep System All technical literature is available
More informationAmoyDx JAK2 Mutation Detection Kit
AmoyDx JAK2 Mutation Detection Kit Detection of V617F mutation in the JAK2 oncogene Instructions For Use For Research Use Only and For Reference Only Instructions Version: B1.2 Date of Revision: June 2016
More informationKit for total DNA isolation from yeast
Cat. No. EM10 Version: 1.2013 Kit for total DNA isolation from yeast EXTRACTME is a registered trademark of BLIRT S.A. www.dnagdansk.com Cat. No. EM10 I. INTENDED USE The EXTRACTME DNA YEAST kit is designed
More informationMag-Bind Stool DNA 96 Kit. M x 96 preps M x 96 preps
Mag-Bind Stool DNA 96 Kit M4016-00 1 x 96 preps M4016-01 4 x 96 preps February 2016 Mag-Bind Stool DNA 96 Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
More informationThermo Scientific GeneJET NGS Cleanup Kit
PRODUCT INFORMATION Thermo Scientific GeneJET NGS Cleanup Kit #K0851, #K0852 www.thermoscientific.com/onebio #K0851, K0852 Lot Expiry Date _ CERTIFICATE OF ANALYSIS Thermo Scientific GeneJET NGS Cleanup
More informationHiPer Gel Extraction Teaching Kit (Column Based)
HiPer Gel Extraction Teaching Kit (Column Based) Product Code: HTBM010 Number of experiments that can be performed: 10 Duration of Experiment Agarose Gel Electrophoresis: 1 hour Protocol: 1 hour Agarose
More informationUser manual Invisorb Spin Plant Mini Kit
User manual Invisorb Spin Plant Mini Kit For genomic DNA purification from up to 100 mg wet and up to 60 mg of dried plant materia lfrom a wide variety of plant species as well as from food samples of
More informationTotal RNA Purification Micro Kit Product # 35300
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Total RNA Purification Micro Kit Product # 35300 Product Insert
More informationHigh Pure PCR Template Preparation Kit for preparation of 100 nucleic acid samples Cat. No
for preparation of 100 nucleic acid samples Cat. No. 1 796 88 Principle Cells are lysed during a short incubation with Proteinase K in the presence of a chaotropic salt (guanidine HCl), which immediately
More informationPureLink Plant Total DNA Purification Kit
USER GUIDE PureLink Plant Total DNA Purification Kit For purification of DNA from plant Catalog Number K1830-01 Document Part Number 25-0757 Publication Number MAN0000478 Revision 2.0 For Research Use
More informationPureSpin DNA Clean Up Kit
PureSpin DNA Clean Up Kit Micro Columns INSTRUCTION MANUAL KIT COMPONENTS For Research Use Only PureSpin DNA Clean Up Kit, Micro Columns w/out Caps (Kit Size) OD2080 (50 Preps.) OD2080-2 (200 Preps.) Storage
More information