MagListo 5M Tissue Total RNA Extraction Kit

Transcription

1 User s Guide MagListo 5M Tissue Total RNA Extraction Kit K-3612 K-3613

2 MagListo TM 5M Tissue Total RNA Extraction Kit MagListo 5M Tissue Total RNA Extraction Kit Kit for the extraction of total RNA from a wide range of Tissue types using MagListo TM User s Guide K-3612 K Version No.: 1.1 ( ) Please read all the information in booklet before using the unit Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: sales@bioneer.co.kr MagListo is a trademark of Bioneer Corporation. Copyright Bioneer Corporation. All Rights Reserved.

3 MagListo TM 5M Tissue Total RNA Extraction Kit Contents I. Overview 1 II. Kit Components 1 III. Storage 2 IV. Intended Use 2 V. Safety Warnings and Precautions 2 VI. Warranty and Liability 3 VII. Technical Assistance 3 VIII. Quality Management 3 IX. Product Specifications 4 X. Principle 4 XI. Materials and Equipment Needed But Not Provided 5 Choice of the right Magnetic Separation Rack 5 XII. Protocols Before you begin 6 RNA Extraction from Animal Tissue 7 RNA Extraction from Cultured Cells 9 ONE-Step RNA Cleanup 12 RNA Cleanup Protocol 13 XIII. Troubleshooting Guide 14 XIV. Ordering Information 15 XV. Explanation Symbols 16 -

4 I. Overview Description MagListo 5M Tissue Total RNA Extraction Kit is an innovative product to extract total RNA from a wide range of tissue types. Additionally this kit can be used for a sample of cultured cells. This kit is designed to be applicable to mini and midi scale extraction of tissue total RNA by using a proper volume of each solution suggested in this User s Guide. MagListo 5M Tissue Total RNA Extraction Kit provides high-quality RNA in unprecedented short period of time. MagListo Magnetic Separation Rack, which is also available from Bioneer (TM-1010 to 1030), will greatly enhance user s convenience and save processing time by removing the necessity of centrifugation and minimizing pipetting steps. Features and Benefits -Magnetic NanoBeads enable the rapid extraction of RNA as short as 10 min. -No need of expensive instruments. -One kit serves either mini or midi scale extraction. Applications Applicable to RNA extraction step for assays requiring RNA, including, but not limited, RT-PCR, cdna synthesis, Northern, dot, and slot blot analyses, Microarrays, and RNAseq II. Kit Components MagListo 5M Tissue Total RNA Extraction Kit *K-3612 ** K-3613 Buffer 1 (Binding) 4 ml x 1 ea 25 ml x 2 ea Buffer 2 (1 st Washing) 5.5 ml x 1 ea 55 ml x 1 ea Buffer 3 (2 nd Washing) 2.0 ml x 1 ea 20 ml x 1 ea Buffer 4 (Elution) 1.8 ml x 1 ea 25 ml x 1 ea Magnetic NanoBeads - RNA 1 ml x 1 ea 1.8 ml x 6 ea *mini - 8 RXNs, midi 1 RXN **mini 100 RXNs, midi 10 RXNs 1

5 III. Storage MagListo 5M Tissue Total RNA Extraction Kit is stored dry at room temperature and can be stored for up to one year if it remains sealed. Buffers may form precipitates during storage. If this occurs, please warm the buffer to 37 until the precipitates are completely dissolved. IV. Intended Use MagListo 5M Tissue Total RNA Extraction Kit is intended for research use only. This kit is not intended for human or veterinary diagnostics use. V. Safety Warnings and Precautions Please inquire BIONEER s Customer Service Center to obtain a copy of the Material Safety Data Sheet (MSDS) for this product. Before, during and after use of this kit as described in this User s Guide, all potentially hazardous materials (i.e. materials that may have come in contact with genetically recombinant samples) including tubes, tips and materials should be processed and disposed of according to applicable and appropriate regulations of the municipality/government in which this product is being used. A user must also be equipped with basic experimental techniques required for correct execution of the experiments described in this User s Guide. Some applications that may be performed with this kit may infringe upon existing patents in certain countries. The purchase of this kit does not include or provide a license to perform patented applications. Users may be required to obtain a license depending on country and application. We do not condone nor recommend the unlicensed use of a patented application. 2

6 VI. Warranty and Liability All BIONEER products are manufactured and tested under strict quality control protocols. BIONEER guarantees the quality of all directly manufactured products during the warranty period of one year from the date of purchase. If any issues are discovered relating to compromise in product quality, immediately contact BIONEER s Customer Service Center (order@bioneer.com). BIONEER does not assume liability for misuse of the product, i.e. usage of the product for any purposes other than its intended purpose as described in the User s Guide. BIONEER assumes liability under the condition that users disclose all information related to the problem to BIONEER in written form within 30 days of occurrence. VII. Technical Assistance At Bioneer, we pride ourselves on being responsive to your needs. If you have any questions or would like to find out more information about MagListo products, please contact us. We look forward to hearing from you! Technical Support For all technical questions and troubleshooting on Bioneer products and applications. Tel: sales@bioneer.com - In North America Tel: support@bioneer.us.com VIII. Quality Management Every aspect of our quality management system from product development, production to quality assurance and supplier qualification meets the world-class standards. Each lot of MagListo 5M Tissue Total RNA Extraction Kit is carefully tested by the quality control team. 3

7 IX. Product Specifications Mini scale Midi scale Starting sample 20 mg tissue or 3 x 10 6 cells 200 mg tissue or 2 x 10 7 cells Extraction time ~ 10 min ~ 15 min Minimum elution volume 50 µl 500 µl Expected purity A 260/280 > 1.9 *RNA content can vary greatly according to tissue type. X. Principle The MagListo 5M Tissue Total RNA Extraction Kit is designed for the extraction of high-quality total RNA from cultured cells. Chemotropic agents in Binding Buffer contains guanidine hydrochloride and guanidine thiocyanate, which remove water molecules around RNA and silica-coated magnetic beads surface resulting in RNA captured by silica coated magnetic beads. The magnetic beads and RNA complexes are pulled and fixed on the tube wall using a magnetic force, followed by being washed with Washing Buffers to remove debris and excessive salts. The captured RNAs are then eluted by Elution Buffer, an aqueous solution with optimal ph. Sample Lysis Binding Washing Elution 4

8 XI. Materials and Equipment Needed But Not Provided 1. Table-top microcentrifuge, 16,000 x g (>13,000 rpm) (mini scale) 2. Centrifuge with rotor capable of 3,000 x g (midi) ml or 2 ml tube (mini scale) ml tube (midi scale) 5. Vortex mixer 6. Absolute ethanol 7. Blow dryer or heat gun or dry oven 8. MagListo Magnetic Separation Rack Choice of the right Magnetic Separation Rack Tube MagListo Magnetic Separation Rack Cat.no 1.5 ml or 2 ml microcentrifuge tube MagListo -2 Magnetic Separation Rack TM ml centrifuge tube MagListo -50 Magnetic Separation Rack TM-1030 (Note) Please refer to the ordering information table on the latter part of the manual which contains the appropriate catalog number for specific size of tubes. 5

9 XII. Protocols Before you begin 1. MagListo 5M Tissue Total RNA Extraction Kit contains chaotropic salt. You should take appropriate laboratory safety precautions and wear gloves and lab goggles when handling. 2. The relative centrifugal force (RCF) is calculated in g as follows: RCF = 1.12 x r x (rpm/1,000) 2 Where r is the radius of a rotor in cm, and rpm is the speed of the rotor in revolutions per minute. 3. Buffer 2 (1 st Washing) and Buffer 3 (2 nd Washing) are supplied as concentrated solutions. Before using for the first time, add absolute ethanol as indicated on the bottles. 4. To inhibit RNase activity, we recommend adding β-mercaptoethanol to Buffer 1 (Binding) before use. Add 10 µl β-mercaptoehanol (>99%) per 1 ml Buffer 1 (Binding). 5. How to use MagListo Magnetic Separation Rack. - Attachment Combine the magnet plate to the stand. Stand 1 Magnet plate - Discard solution Discard solution by inverting the MagListo rack. The silicone immobilizer inside the stand holds the tubes from falling in an upside down position. When discard solution, invert the rack completely for the solution not to smear on the rack. 2 - Detachment Push up the magnet plate gently. 3 6

10 RNA Extraction from Animal Tissue in Mini ( 20 mg of tissue) or Midi ( 200 mg of tissue) Scale Tissue Lysis & homogenization (Either Step 1 or Step 2 & 3) 1. Disruption and homogenization using a rotor-stator homogenizer : Place the weighed (fresh, frozen, or RNAlater -stabilized) tissue in a suitable-sized vessel. Add 400 µl (mini) / 4 ml (midi) of Buffer 1 (Binding) to the tissue sample. Immediately disrupt and homogenize the tissue using a conventional rotor-stator homogenizer until it becomes uniformly homogeneous and transfer it to a new 2 or 1.5 ml tube (go to step 4). 2. Disruption using a mortar and pestle followed by homogenization using a needle and syringe: Immediately place the weighed (fresh, frozen, or RNAlater -stabilized) tissue in liquid nitrogen, and grind thoroughly with a mortar and pestle. Decant tissue powder and liquid nitrogen into an RNase-free, liquid-nitrogen-cooled 2 ml tube. Allow the liquid nitrogen to evaporate, but do not allow the tissue to thaw (go to step 3). 3. Add 400 µl (mini) / 4 ml (midi) of Buffer 1 (Binding) to the tissue sample, then completely homogenize the tissue by pipetting or vortexing for at least 1 min. (Note) Insufficient homogenization can decrease the RNA purification yield, and also cause clogging of Magnetic NanoBeads in the following steps. For the sufficient homogenization of the lysate, make the lysate passed through blunt 20-gauge needle (0.9 mm diameter) 5 to 10 times. A. For mini scale extraction, please transfer the lysate to a 2 ml (or 1.5 ml) tube. B. For midi scale extraction, please transfer the lysate to a 50 ml tube. RNA binding 4. Add 200 µl (mini) / 2 ml (midi) of absolute ethanol to the tube and mix by vortexing or shaking. 5. Add 100 µl (mini) / 1 ml (midi) of Magnetic NanoBeads solution to the tube and mix by vortexing or shaking. (Note) Please vortex Magnetic NanoBeads solution well before use. 6. Place the tube in MagListo -2 (mini) / MagListo -50 (midi) Magnetic Separation Rack with the magnet plate attached and invert the tube 3-4 times gently until the beads tightly bind to magnet. 7. Keeping the tubes in MagListo rack with the magnet attached, discard the supernatant and remove the remaining supernatant carefully using a paper towel by blotting. (Optional: If performing optional ONE Step RNA Clean up, follow steps (page 12) after performing this step.) 7

11 Washing 8. Detach the magnet plate from MagListo rack. Add 800 µl (mini) / 8 ml (midi) of Buffer 2 (1 st Washing) to the tube. Close the cap and mix by vortexing or shaking until the beads are fully resuspended. 9. Place the tubes in MagListo rack with the magnet plate attached and invert the tube 3-4 times gently until the beads tightly bind to the magnet. 10. Keeping the tubes in the MagListo rack with the magnet attached, discard the supernatant and completely remove the remaining supernatant using a paper towel by blotting. 11. Detach the magnet plate from MagListo rack. Add 800 µl (mini) / 8 ml (midi) of Buffer 3 (2 nd Washing) to the tube. Close the cap and mix by vortexing or vigorous shaking until the beads are fully resuspended. 12. Repeat the above steps 9 and 10 for additional washing. 13. Detach the magnet plate from MagListo rack. Add 800 µl (mini) / 8 ml (midi) of absolute ethanol to the tube. Close the cap and mix by vortexing or shaking until the beads are fully resuspended. 14. Repeat the above steps 9 and 10 for additional washing. Drying 15. With a heat gun or a blow dryer, completely dry the beads with the tube open 3 cm away from the top of the tube. (mini: >1 min) (Note) Alternatively, the beads can be dried with a dry oven at 65 for current times. (mini: >5 min, midi: >15 min) Please use the clean bench during the drying procedure to prevent RNase or other aerosol contamination. Elution 16. Add >50 μl (mini) / >500 μl (midi) of Buffer 4 (Elution) to the tube with the magnet plate detached and resuspend RNA by vortexing or pipetting. 17. Incubate the tube at for 2 min. Warm up the tube with a heat block, a heat gun or a blow dryer. 18. Vortex the tube for 15 sec. 19. Attach the magnet plate to MagListo rack and invert the tube 3-4 times gently until the beads tightly bind to the magnet. 20. Keeping the tube in MagListo rack with the magnet attached, carefully take the supernatant containing RNA to a sterile microcentrifuge tube. 21. Discard the used Magnetic NanoBeads. Do not reuse the beads. 8

12 RNA Extraction from Cultured Cells in Mini ( 3 x 10 6 cells) or Midi ( 2 x 10 7 cells) Scale Harvest of cells 1. Cells grown in suspension: Count the cell number, then centrifuge the proper number of cells at 300 xg for 5 min. Discard supernatant carefully and go to lysis & homogenization (go to step 3). 2. Cells grown in a monolayer: There are 2 different ways to collect cells grown in a monolayer. 2a. Direct cell lysis on the Culture Dish: Completely remove Cell Culture Medium and go to lysis & homogenization (go to step 3). (Remaining medium may inhibit the RNA extraction) 2b. Harvesting cells with trypsin: Remove Cell Culture Medium and wash the monolayer with DPBS. Add 0.1%-0.25% typsin to the washed cell monolayer. When the cells are detached, add Cell Culture Medium to inactivate the typsin. Transfer the cells into a RNase-free tube and centrifuge at 300 xg for 5 min. Discard supernatant carefully and go to lysis & homogenization (go to step 3). Lysis & homogenization 3. Add 400 µl (mini) / 4 ml (midi) of Buffer 1 (Binding) to the cell culture dish or the collected cell pellet and completely homogenize the cells by pipetting or vortexing for at least 1 min. (Note) Insufficient homogenization can decrease the RNA purification yield, and also cause clogging of Magnetic NanoBeads in the following steps. For the sufficient homogenization of the lysate, make the lysate passed through blunt 20-gauge needle (0.9 mm diameter) 5 to 10 times. A. For mini scale extraction, please transfer the lysate to a 2 ml (or 1.5 ml) tube. B. For midi scale extraction, please transfer the lysate to a 50 ml tube. RNA binding 4. Add 200 µl (mini) / 2 ml (midi) of absolute ethanol to the tube and mix by vortexing or shaking. Add 100 µl (mini) / 1 ml (midi) of Magnetic NanoBeads solution to the tube and mix by vortexing or shaking. (Note) Please vortex Magnetic NanoBeads solution well before use. 5. Place the tube in MagListo -2 (mini) / MagListo -50 (midi) Magnetic Separation Rack with the magnet plate attached and invert the tube 3-4 times gently until the beads tightly bind to magnet. 6. Keeping the tubes from MagListo rack with the magnet attached, discard the supernatant and remove the remaining supernatant using a paper towel by blotting. (Optional: If performing optional ONE Step RNA Cleanup, follow steps (page 12) after performing this step) 9

13 Washing 7. Detach the magnet plate from MagListo rack. Add 800 µl (mini) / 8 ml (midi) of Buffer 2 (1 st Washing) to the tube. Close the cap and mix by vortexing or shaking until the beads are fully resuspended. 8. Place the tubes in MagListo rack with the magnet plate attached and invert the tube 3-4 times gently until the beads tightly bind to the magnet. 9. Keeping the tubes in the MagListo rack with the magnet attached, discard the supernatant and completely remove the remaining supernatant using a paper towel by blotting. 10. Detach the magnet plate from MagListo rack. Add 800 µl (mini) / 8 ml (midi) of Buffer 3 (2 nd Washing) to the tube. Close the cap and mix by vortexing or vigorous shaking until the beads are fully resuspended. 11. Repeat the above step 9 and 10 for additional washing. 12. Detach the magnet plate from MagListo rack. Add 800 µl (mini) / 8 ml (midi) of absolute ethanol to the tube. Close the cap and mix by vortexing or shaking until the beads are fully resuspended. 13. Repeat the above step 9 and 10 for additional washing. Drying 14. With a heat gun or a blow dryer, completely dry the beads with the tube open 3 cm away from the top of the tube. (mini: >1 min) (Note) Alternatively, the beads can be dried with a dry oven at 65 for current times. (mini: >5 min, midi: >15 min) Please use the clean bench during the drying procedure to prevent RNase or other aerosol contamination. Elution 15. Add >50 μl (mini) / >500 μl (midi) of Buffer 4 (Elution) to the tube with the magnet plate detached and resuspend by vortexing or pipetting. 16. Incubate the tube at for 2 min. Warm up the tube with a heat block, a heat gun or a blow dryer. 17. Vortex the tube for 15 sec. 18. Attach the magnet plate to MagListo rack and invert the tube 3-4 times gently until the beads tightly bind to the magnet. 19. Keeping the tube in MagListo rack with the magnet attached, carefully take the supernatant containing RNA to a sterile microcentrifuge tube. 20. Discard the used Magnetic NanoBeads. Do not reuse the beads. 10

14 Summary of reagent volumes required in each step of Tissue Total RNA Extraction. Step Buffer Mini scale ( 20 mg tissue) Midi scale ( 200 mg tissue) Tissue Lysis Buffer 1 (Binding) 400 μl 4 ml Addition of Ethanol Absolute ethanol 200 μl 2 ml RNA Binding Magnetic NanoBeads - RNA 100 μl 1 ml 1 st Washing Buffer 2 (1 st Washing) 800 μl 8 ml 2 nd Washing Buffer 3 (2 nd Washing) 800 μl 8 ml 3 rd Washing Absolute ethanol 800 μl 8 ml Elution Buffer 4 (Elution) >50 μl >500 μl Summary of reagent volumes required in each step of Cell Total RNA Extraction Step Buffer Mini scale ( 3 x 10 6 cells) Midi scale ( 2 x 10 7 cells) Cell Lysis Buffer 1 (Binding) 400 μl 4 ml Addition of Ethanol Absolute ethanol 200 μl 2 ml RNA Binding Magnetic NanoBeads - RNA 100 μl 1 ml 1 st Washing Buffer 2 (1 st Washing) 800 μl 8 ml 2 nd Washing Buffer 3 (2 nd Washing) 800 μl 8 ml 3 rd Washing Absolute ethanol 800 μl 8 ml Elution Buffer 4 (Elution) >50 μl >500 μl 11

15 ONE-Step RNA Clean Up Protocol This protocol is to remove DNA for use in certain applications. 1. Detach the magnet plate from MagListo rack. Add 800 µl (mini) / 8 ml (midi) of absolute ethanol to the tube. Close the cap and mix by vortexing or shaking until the beads are fully resuspended. 2. Place the tube in MagListo -2 (mini) / MagListo -50 (midi) with the magnet plate attached and invert the tube 3-4 times gently until the beads tightly bind to the magnet. 3. Keeping the tube from MagListo rack with the magnet attached, discard the supernatant and remove the remaining supernatant using a paper towel by blotting. 4. With a heat gun or a blow dryer, completely dry the beads with the tube open 3 cm away from the top of the tube. (mini: >2 min, midi: >5 min) (Note) Alternatively, the beads can be dried with a dry oven at 65 for current times. (mini: > 5 min, midi: >15 min) Please use the clean bench during the drying procedure to prevent RNase or other aerosol contamination. 5. Add DNAse Reaction Buffer and RNase-Free DNase to the tube. (mini: up to 70 µl, midi: up to 700 µl) 6. Detach the magnet plate from MagListo rack and Close the cap and mix by vortexing or shaking until the beads are fully resuspended. 7. Place on the benchtop (20 30 C) for 20 min. 8. Add 300 µl (mini) / 3 ml (midi) scale extraction of Buffer 2 (1 st Washing) and 300 µl (mini) / 3 ml (midi) scale extraction of absolute ethanol to the tube. Close the cap and mix by vortexing or shaking until the beads are fully resuspended. 9. Place the tube in MagListo rack with the magnet plate attached and invert the tube 3-4 times gently until the beads tightly bind to the magnet. 10. Keeping the tube in MagListo rack with the magnet attached, discard the supernatant and remove the remaining supernatant using a paper towel by blotting. 11. Go to 2 nd Washing step 11 (Page 8, 10) and follow the instruction described above for the remaining steps. 12

16 RNA Clean up Protocol This protocol is to remove enzymes, buffers, or chemical inhibitors and concentrate RNA for use in certain applications. 1. Transfer RNase-free water into RNA sample to a volume of 100 µl. 2. Add 100 µl of Buffer 1 (Binding) and mix thoroughly. 3. Add 200 µl of absolute ethanol and mix well. 4. Add 100 µl of Magnetic NanoBeads solution to the tube and mix by vortexing or shaking (Note) Please vortex Magnetic NanoBeads solution well before use. 5. Place the tube in MagListo rack with the magnet plate attached and invert the tube 3-4 times gently until the beads tightly bind to the magnet. 6. Keeping the tube in MagListo rack with the magnet attached, discard the supernatant and remove the remaining supernatant using a paper towel by blotting. 7. Go to 1 st Washing step 8 (Page 8, 10) and follow the instruction described above for the remaining steps. 13

17 XIII. Troubleshooting Guide Comments and suggestions Insufficient homogenization: (Refer to Page 7) Add Buffer 1 (Binding) to the tissue (cells) and completely homogenize the tissue (cells) by vortexing for at least 1 min or increase the vortexing time for the sufficient homogenization up to 5 to 10 minutes. Use of an excess amount of sample: Use the proper amount of samples according to the instructions of this User s Guide. Low or no yield Insufficient beads dried: Beads must be completely dried. Any leftover of ethanol can decrease the RNA purification yield. Insufficient incubation during the Elution step: RNA could remain attached to the beads if the incubation time is not enough. To prevent this, please repeat the elution process with the increased incubation time. Incomplete removal of cell culture medium: The best approach is to remove the medium as much as possible. Any leftover in the medium can lead to the inhibition of RNA extraction. Aggregation of Magnetic NanoBeads RNA degradation Inhibition of downstream enzymatic reactions Low A 260/280 ratio Insufficient homogenization: (Refer to Page 7) Add Buffer 1 (Binding) to the tissue (cells) and completely homogenize the tissue (cells) by vortexing for at least 1min or increase the vortexing time for the sufficient homogenization. Use of an excess amount of sample: Use the proper amount of samples according to the instructions of this User s Guide. RNase contamination: Use a heat gun or a blow dryer in a clean bench to prevent the contamination of RNase in the air. Use RNase-free pipette tips and change the gloves frequently. Inappropriate handling of harvested samples: If the extraction is not performed right after harvesting the samples, store the samples at -80 or lysis the samples with Buffer 1 (Binding) and then store at -80. Insufficient beads dried: Beads must be completely dried. Any leftover in the ethanol can decrease the RNA purification yield. Insufficient washing during the beads suspension process: Insufficient suspension of beads during the washing step causes salts to remain in the purified RNA. Recommendation is to suspend the beads thoroughly during the washing process. Insufficient beads dried: Beads must be completely dried. Any leftover in the ethanol can decrease the RNA purification yield. Insufficient washing during the beads suspension process: Insufficient suspension of beads during the washing step causes salts to remain in the purified RNA. Recommendation is to suspend the beads thoroughly during the washing process. 14

18 XIV. Ordering Information Cat no. Product Description Size K-3601SM/ K-3601/ K-3600 K-3602/ K-3603 K-3604/ K-3605 K-3606/ K-3607 K-3608/ K-3609 K-3610/ K-3611 K-3612/ K-3613 K-3614/ K-3615 MagListo 5M Plasmid Extraction Kit MagListo 5M Genomic DNA Extraction Kit MagListo 5M Plant Genomic DNA Extraction Kit MagListo 5M Gel Extraction Kit MagListo 5M PCR Purification Kit MagListo 5M Cell Total RNA Extraction Kit MagListo 5M Tissue Total RNA Extraction Kit MagListo 5M Forensic Sample DNA Extraction Kit 8 rxn / 100 rxn/ 500 rxn (mini*) 8 rxn / 100 rxn (mini*) 8 rxn / 100 rxn (mini*) 8 rxn / 100 rxn (mini*) 8 rxn / 100 rxn (mini*) 8 rxn /100 rxn (mini*) 8 rxn /100 rxn (mini*) 8 rxn /100 rxn (mini) TM-1000 MagListo -8Ch Magnetic Separation Rack 1 ml tube x 8 holes TM-1010 MagListo -2 Magnetic Separation Rack 2 ml tube x 8 holes TM-1020 MagListo -15 Magnetic Separation Rack 15 ml tube x 6 holes TM-1030 MagListo -50 Magnetic Separation Rack 50 ml tube x 3 holes TM-1040 MagListo -96 Magnetic Separation Rack 96 well plate x 1 ea TM-1100 MagListo Magnetic Separation Rack Bundle Set Each of MagListo TM -2, -15, -50, and -96 (4 racks) K-3601-A Blow Dryer 1 ea HT-15-NG 1.5 ml microcentrifuge tube 500 ea / pk HT-20-NG 2 ml microcentrifuge tube 500 ea / pk ml tube 500 ea / pk ml tube 500 ea / pk ml tube with 8-cap strip 100 ea / pk 15

19 XV. Explanation Symbols Catalog Number Contains sufficient for (n) tests USE BY Consult Instruction For Use Caution, Batch code consult accompanyi ng Temperatu re Limitation Research Use Only documents Manufactur er Caution, Potential Biohazard DO NOT REUSE 16

20 [Note] 17

21 8-11 Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302, Republic of Korea (Korea: ) Marina Village PKWY, Suite 110, Alameda, CA 94501, USA (Toll-free) us.bioneer.com Korea Bio Park BLDG #B-702, 700 Daewangpangyo-ro, Bundang-gu, Seongnam-si Gyeonggi-do, 13488, Republic of Korea