Basic Biosafety Principles. EH&S Academy Brenda J. Wong, UCSD Biosafety Officer October 2009

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1 Basic Biosafety Principles EH&S Academy Brenda J. Wong, UCSD Biosafety Officer October 2009

2 What is Biosafety? Safety from exposure to Infectious Agents Smallpox

3 What is Biosafety? Sunday, Sep. 20, 2009 Did the Plague Kill Illinois Scientist? By AP (AP / CHICAGO) The University of Chicago Medical Center says the infection that killed a scientist may be connected to bacteria he researched that causes the plague. The university said Saturday that its researcher studied the genetics of harmful bacteria including Yersinia pestis, which causes the illness. He died Sept. 13. His name and age haven't been released The medical center says the bacteria he worked with was a weakened strain that isn't known to cause illness in healthy adults. The strain was approved by the Centers for Disease Control and Prevention for laboratory studies. An autopsy found no obvious cause of death but did find the presence of the bacteria. More tests are planned. No other illnesses have been reported.

4 Biosafety in Various Disciplines Biosafety is related to several fields ECOLOGY: referring to imported life forms not indigenous to the region (Reggie the alligator) AGRICULTURE: reducing the risk of alien viral or transgenic genes, or prions such as BSE/"MadCow ; reducing the risk of food bacterial contamination MEDICINE: referring to organs or tissues from biological origin, or genetic therapy products, virus; levels of lab containment protocols BSL-1, 2, 3, 4 in rising order of danger CHEMISTRY: i.e., nitrates in water, PCB levels affecting fertility EXOBIOLOGY: i.e., NASA's policy for containing alien microbes that may exist on space samples - sometimes called "biosafety level 5"

5 Biosafety in Academic Research Research Universities: Promoting safe laboratory practices, and procedures; proper use of containment equipment and facilities; provides advice on laboratory design and risk assesment of experiments involving infectious agents, rdna in-vitro and in-vivo. Bottom Line: Risk & Containment

6 Biohazard Symbol Charles Baldwin at National Cancer Institute at NIH. Symbol to be memorable but meaningless so it could be learned. Blaze orange most visible under harsh conditions

7 Biosafety Issues Laboratory Safety Bloodborne pathogens (BBP) Recombinant DNA (rdna) Biological waste disposal Infectious substance and diagnostic specimen shipping

8 Biosafety Issues (con t.) Respiratory Protection Bioterrorism and Select agents Mold and indoor air quality Occupational safety and health in the use of research animals Biohazards used in animal models

9 Biohazardous Materials Viruses Bacteria Fungi Chlamydiae/Rickettsiae Prions Recombinant DNA

10 Biohazardous materials Transgenic Plants, Animals and Insects

11 Transgenic Insects

12 Biohazardous Materials Human and Primate Cells, Tissues, and Body Fluids Brain Tissue from Demented Patients Viral Vectors Replication deficient viruses

13 Biosafety Concepts Biosafety In Microbiological and Biomedical Laboratories BMBL (acronym) CDC/NIH Publication Safety Guidelines Regulations of Institution receives NIH funding Code of Practice and Gold Standard in Industry anl Gold Standard Clinical & Research Lab. HHS Publication No. (CDC)

14 The New BMBL Early print edition. Emphasis on Risk & Containment

15 The BMBL continues to be published by the CDC and the NIH 5th edition is now at the printers sfty/bmbl5/bmbl5toc.htm Biosafety Concepts The BMBL

16 Are the NIH Guidelines Optional? Guidelines does not mean optional They are a term and condition of NIH funding for recombinant DNA research. From Kathryn Harris, NIH, OBA

17 Biosafety Concepts from the BMBL Principles of Biosafety Practice and Procedures Standard Practices Special Practices & Considerations Safety Equipment Facility Design and Construction Increasing levels of protection

18 Principles of Biosafety Biosafety Levels 1-4 (BSL) Increasing levels of employee and environmental protection Guidelines for working safely in research & medical laboratory facilities Animal Biosafety Levels 1-4 (ABSL) Laboratory animal facilities Animal models that support research Guidelines for working safely in animal research facilities

19 Biosafety Concepts The BMBL (1) Standard Microbiological Practices Most important concept / Strict adherence Aware of potential hazard Trained & proficient in techniques Supervisors responsible for: Appropriate Laboratory facilities Personnel & Training Special practices & precautions Occupational Health Programs

20 Biosafety Issues The BMBL (2) Safety Equipment Primary Containment Barrier Minimize exposure to hazard Prevent contact / Contain aerosols Engineering controls/ equipment Personal Protective Equipment (PPE) Gloves, gowns, Respirator, Face shield, Booties Biological Safety Cabinets Covered or ventilated animal cage systems

21 Biosafety Concepts The BMBL (3) Facility Design and Construction Secondary Barrier/ Engineering controls Contributes to worker protection Protects outside the laboratory Environment & Neighborhood Ex. Building & Lab design, Ventilation, Autoclaves, Cage wash facilities, etc.

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23 Laboratory Design Warehouse Type Lab

24 Discussion What are some of the negatives and positives of this open lab concept?

25 Biosafety Level-1 Concepts of Biosafety Biosafety Level-1 (BSL-1 or ABSL-1) Well characterized agents Agents not known to cause disease (in healthy human adults; now healthy immunocompetent adults) Prophylactic treatment available Open bench procedures Animals in open cage system or open environment (outdoors) Good laboratory practices

26 Risk Group 1 Agents E.coli K-12 Transgenic Plants Plasmids Fungi Mold Yeast

27 BSL-1 Practices Bench-top work allowed Daily Decontamination Manual pipetting Required Handwashing Red bag waste Bio cabinet not required (unless creating aerosols) 2 containment

28 Risk Group 2 Agents Human or Primate Cells Herpes Simplex Virus Replication Incompetent Attenuated Human Immunodeficiency Virus Patient specimens

29 Practices & Procedures Agents associated w/ human disease Treatment for disease available BSL-2 Practices Concepts of Biosafety Agent poses moderate hazard to personnel and environment Direct contact or exposure Percutaneous exposure Scratch, Puncture, Needle stick Mucus membrane exposure Eyes, Mouth, open cut

30 BSL-2 Practices Limited access to lab when work in progress Daily decontamination Mechanical pipetting Labcoat, safety glasses and gloves required Red bag & sharps containers required

31 BSL-2 Practices (con t) Biohaz. Sign posted at entrance to lab Label all equipment (incubators, freezers, etc.) TC room negative air flow Documented training Baseline serology or prevaccination may be required

32 Risk Group 3 Agents Human Immunodeficiency Virus Mycobacterium tuberculosis Coxiella burnetii

33 Biosafety Level 3 Working in High Containment Biosafety Level-3 (BSL-3 or ABSL-3) Indigenous or exotic agents Aerosol transmission Serious health effects Treatment may or may not exist

34 BSL-3 Practices Public access NOT permitted Daily decontamination after spill and upon completion of experiment Autoclave required and waste is disposed at the end of day Required foot activated handwashing sink and controls No sharps unless absolutely necessary

35 BSL-3 Practices (con t) Aerosol minimization procedures required Wrap around disposable clothing is required. Specialized equipment may be required depending upon procedures Biohaz. Signs and labels posted Air flow from low hazard to high hazard Pressure Mapping

36 BSL-3 Practices (con t) Bench top work not permitted Documented training and personnel competency certification (for BSL-3 procedures) Baseline serology Spills report immediately and treat accordingly Vaccinations/post exposure protocols and SOP s, Biosafety Manual, Biosafety Officer

37 UCSD s BSL-3

38 Biosafety Level-4 Working in High Containment Biosafety Level-4 Builds on BSL-3/ ABSL-3 practices Maximum containment facilities Pressurized Containment Suite BSL-3 + Class III Biosafety Cabinet Chemical decontamination showers Liquid effluent collection / decontamination No BSL-4 labs exist at UCSD

39 Biosafety Level 4 Lassa Fever Virus Ebola Hemmorrhagic Fever Virus Marburg Virus Herpes B Virus

40 Biosafety Concepts Working in High Containment Biosafety Level-4 (BSL-4 or ABSL-4) Dangerous/exotic agents Life threatening disease Aerosol transmission Agents of unknown risk of transmission or health affects No known treatment

41 Animal Biosafety Level-4 Working in High Containment

42 General Good Lab Technique Hygienic Practices No Smoking, Eating, Applying cosmetics, lip balm, contacts Wash hands after procedures Decontaminate lab bench before and after work

43 General Operational Practices Proper attire Minimum lab coat, safety glasses, gloves Plan your work Know in advance what you are working with Read available resources (MSDS)

44 Animal Containment Points CDC CDC & UCSD Courtesy of Paul Vinson, CDC

45 Discussion # 2 Based on what you know about Biosafety Levels, Practices and Operational Controls, what are some discussion issues for conducting Biohazard risk assessments? How do you approach risks when addressing a particular organism?

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47 Risk Assessment In-Vitro In-Vivo Human Clinical Trial

48 Addressing Risk Assessments What is the organism? Is it Wild-type, attenuated, irradiated, or chemically treated? Look at kill data or kill curves. What is the max. concentration, volume, infectious dose? What is the work space like? Aerosolizing procedures? How do they contain their aerosols?

49 Risk Assessment, con t Are personnel trained? Do personnel understand the organism, infectious dose and symptoms? What are their experimental procedures? Will they be transporting the material? Shipping intra, interstate or international? Are they doing tissue culture? Do they have adequate containment equipment? Tom Pugh

50 Risk Assessment, Con t Are they doing this work in-vivo? Have you consulted and discussed this with the Vets and IACUC to determine special needs and housing? Waste issues addressed? Pregnancy issues with the organisms?

51 Risk Assessment, con t Do they share their Tissue Culture room? Do they have more than 1 Biosafety Cabinet? Occupational Health informed and set up to receive patient or offer counseling?

52 Accidental Spills Evacuate area, alert personnel and cordon off so that aerosols may settle Don PPE; Cover with paper towels and apply bleach (1 part bleach : 9 parts water Allow min contact time Wipe up working towards center Use tongs if broken glass is involved Is Recombinant DNA involved?

53 First Aid Measures Splash to Eye or Needlestick Injury Rinse thoroughly for 15 minutes at the eyewash or sink Call Occupational Medicine Call EH&S to report exposure

54 What Helps?

55 Resources UCSD Biosafety: Biological Safety MSDS: NIH BMBL: See Handout for additional resources..

56 Questions? Brenda Wong Cell: Additional Resources: Biosafety Resources.doc

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