COURSE OUTLINE. School of Engineering Technology and Applied Science. Biological Technology Industrial Microbiology. Advanced Biotechnology
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1 COURSE OUTLINE SCHOOL: School of Engineering Technology and Applied Science DEPARTMENT: Applied Biological and Environmental Science (ABES) PROGRAM: Biological Technology Industrial Microbiology COURSE TITLE: Advanced Biotechnology COURSE CODE: BI 305 TOTAL COURSE HOURS: 75 (15 weeks X 5 hours) PRE-REQUISITES/CO-REQUISITES: CH 222 and BI 312 COURSE ELIGIBILITY FOR PRIOR LEARNING ASSESSMENT AND RECOGNITION (PLAR): Yes ORIGINATED BY: REVISED BY: Paula Demacio, Ph.D, August 2007 Paula Demacio, Ph.D DATE: December 2008 APPROVED BY: Semester: 2009 Winter Chairperson/Dean Students should keep all course outlines for each course taken at Centennial College. These may be used to apply for transfer of credit to other educational institutions. A fee may be charged for additional or replacement copies. 1/14/2009 THIS COURSE ADHERES TO ALL COLLEGE POLICIES (See College Calendar) Page 1
2 COURSE DESCRIPTION: Classroom and laboratory instruction will focus on the theory and practical aspects of advanced biotechnology topics and their applications in commercial and academic research initiatives. Major lecture topics will include genetic engineering, bioinformatics, the Human Genome Project, and pharmacogenetics. The lecture portion of this course will be provided in mixed-mode delivery format, such that 2 hours per week will be comprised of inclass lecture material, and 1 hour per week will be online/self-directed learning. In the laboratory component of the course, the student will integrate concepts and techniques acquired in this and previous courses and apply these to topics such as advanced PCR applications, protein quantification and analysis, ELISA and microarray analysis. COURSE LEARNING OUTCOMES: 1. Describe the theory and methods behind the generation of genetically engineered plants and animals. 2. Identify advantages, disadvantages and ethical issues surrounding genetic engineering. 3. Define pharmacogenetics and explain the relationship between drugs, diseases and genes. 4. Demonstrate the ability to use web-based bioinformatics software to retrieve, identify and analyze DNA and protein sequences. 5. Describe how protein function is determined by structure and use advanced protein visualization software to manipulate 3-D protein structures. 6. Describe the theory behind advanced PCR applications and successfully amplify and analyze multiple DNA samples using the Polymerase Chain Reaction. 7. Select an advanced biotechnology topic and present a well-organized scientific poster to the college community. 8. Simulate a gene-expression microarray experiment and successfully interpret results. ESSENTIAL EMPLOYABILITY SKILLS (EES): This course supports the students ability to: Communicate clearly, concisely, and correctly in the written, spoken, and visual form that fulfills the purpose and meets the needs of the audience. Respond to written, spoken, or visual messages in a manner that ensures effective communication. Locate, select, organize, and document information using appropriate technology and information systems. Analyze, evaluate, and apply relevant information from a variety of sources. Show respect for the diverse opinions, values, belief systems, and contributions of others. Interact with others in groups or teams in ways that contribute to effective working relationships and the achievement of goals. Manage the use of time and other resources to complete projects. Take responsibility for one s own actions, decisions, and consequences. PRIOR LEARNING ASSESSMENT & RECOGNITION PROCESS (ES): This course is eligible for PLAR through the Registrar and SETAS offices. Assessment of portfolio and/or testing may be discussed with faculty. EVALUATION & GRADING SYSTEM: Lecture Midterm Test 20% Assignments 15% Final Exam 25% Lab Reports/Assignments/Quizzes/Performance 30% Biotechnology Poster 10% Students must achieve passing grades in both the lecture and lab components in order to successfully complete this course. 1/14/2009 THIS COURSE ADHERES TO ALL COLLEGE POLICIES (See College Calendar) Page 2
3 STUDENT ACCOMMODATION: All students have the right to study in an environment that is free from discrimination and/or harassment. It is College Policy to provide accommodation based on grounds defined in the Ontario Human Rights Code. Accommodation may include changes or modifications to standard practices. Students with disabilities who require academic accommodations must register with the Centre for Student with Disabilities. Please see the Centre for Students with Disabilities for details. Students requiring accommodation based on human rights grounds should talk with their professors as early as possible. Details are available on the Centennial College website ( ). If students are unable to write an examination due to a medical problem or unforeseen family problems, they should immediately contact their professor or program Chair for advice. In exceptional and well documented circumstances (e.g. unexpected family problems, serious illness, or death of a close family member), students should be able to write a make-up examination to replace an examination missed. TEXT AND OTHER INSTRUCTIONAL/LEARNING MATERIALS: No Required Text Lab Materials will be made available to students via Blackboard. Suggested Reading: DNA Science, A First Course (2 nd Edition), David A. Micklos, Greg A. Freyer with David A. Crotty, Cold Spring Harbor Laboratory Press, 2003, ISBN # MOLECULAR BIOTECHNOLOGY Principles and Applications of Recombinant DNA 2 nd Ed., B.R. Glick and J.J. Pasternak, American Society of Microbiology ISBN: USE OF DICTIONARIES Dictionary use is not permitted in test or examination settings, but may be used during regular class work. 1/14/2009 THIS COURSE ADHERES TO ALL COLLEGE POLICIES (See College Calendar) Page 3
4 College Policies POLICY STATEMENTS The following statements are selected from Centennial College policies approved by the Board of Governors. Student Responsibilities Students are expected to know the contents of the course outline and to discuss with the professor any areas where clarification is required. Students should keep all course outlines for each course taken at Centennial College. These may be used to apply for transfer of credit to other educational institutions. A fee may be charged for additional or replacement copies. Other Policies Students should familiarize themselves with all College Policies that cover students rights, responsibilities, and the Academic Appeal process. For further information, consult the Academic Matters Section in the full-time and Continuing Education calendars. The Academic Appeal Application form is available from any Enrolment Services Office. Proof of Student Status Students must produce official photo identification at any time during the semester when requested to do so by any professor. (The official piece is the Centennial Student Card.) Continuing Education students do not have Centennial Student Cards, and so they may use other forms of photo identification, such as a driver s license, health card, or other government-issued photo identification. Final Examinations When writing a test or examination, students must put their official photo-id cards in full view for review by the invigilator. Students who do not have official photo-id will be permitted to write the examination with a substitute photo-id, but they will be required to produce photo-id at the program or department office within 24 hours or the next business day following the examination, or else the examination results will be void. More Final Examination Policies are available at Academic Progression Policy for Diploma and Certificate Programs: College Academic Standings will be applied. Please see Academic Policies and Procedures, Full-Time Calendar. Faculty Consultation Professors are available to see students outside of class time. Students can contact professors via voice mail, , or through their program or department office. Information regarding how to contact teachers will be provided at the beginning of the course and is also available in the program or department office. Human Rights Statement It is the policy of the College that all programs will strive for a learning, teaching, and working environment that promotes inclusion, understanding, and respect for all students and employees, consistent with the Ontario Human Rights Code and Centennial College's Statement of Diversity. 1/14/2009 THIS COURSE ADHERES TO ALL COLLEGE POLICIES (See College Calendar) Page 4
5 TOPICAL OUTLINE WEEK TOPIC/CONTENT LEARNING OUTCOMES 1 Genetic Engineering: Transgenic Plants: Production and Applications Risks and Advantages of Genetic Engineering Describe the theory and methods (Agrobacterium transformation, Gene Gun, protoplast injection and microfibres) behind the generation of genetically engineered plants. Discuss applications, advantages, disadvantages and ethical issues surrounding transgenic plants. 2 Genetic Engineering: Knockout and Transgenic Mice Describe the theory and methods behind the production of genetically engineered mice. Compare and contrast the experimental objectives and technical approaches of transgenic versus knockout mice. Discuss applications, advantages, disadvantages and ethical issues surrounding transgenic animals. 3 Protein-Protein Interactions: Yeast Two-Hybrid Assay Explain the basic premise/theory behind the yeast two-hybrid assay. Describe the experimental steps of a yeast two-hybrid assay (including specific plasmid and yeast strain characteristics, colony selection strategies, plasmid isolation techniques, etc.). Discuss advantages and disadvantages of the yeast two-hybrid approach. 4 Bioinformatics, The Human Genome Project, Biological Databases 5 Single Nucleotide Polymorphisms and Pharmacogenetics 6 Complete unfinished lecture material/review 7 Test #1 20% Define bioinformatics and state the main goals of the field. Outline the goals, insights/findings, and ethical aspects of the Human Genome Project. Compare and contrast the sequencing strategies of the public versus private sequencing efforts in the Human Genome Project. Discuss the role of alternative splicing in human genomic/proteomic complexity. Define a SNP. Use an example to explain how SNPs can alter protein structure and function. Define pharmacogenetics and explain how SNP analysis is involved in personalized medicine. 8 MIcroarrays Analyze the microarray approach to gene expression analysis, including both the theory and methods behind gene chip production, mobile probe preparation, experimental design, and results analysis. Simulate a gene-expression microarray experiment using online and paper simulations, and successfully interpret results. Reading Week 1/14/2009 THIS COURSE ADHERES TO ALL COLLEGE POLICIES (See College Calendar) Page 5
6 WEEK TOPIC/CONTENT LEARNING OUTCOMES 9 Guest Speaker: Biotechnology and the Pharmaceutical Industry 10 Field Trip 11 Proteomics Biotechnology Poster Day Explain how protein function is determined by structure. Retrieve and manipulate 3-D protein structures from online databases. Select an advanced biotechnology topic and present a well-organized scientific poster to the college community. 12 Bioinformatics Special Topics Discuss the theory, methodology and rationale underlying genomics applications such as barcoding life, metagenomics, and computer-aided drug design (CADD). Outline the steps in a protein expression profiling experiment, explain its benefits in a diagnostic setting, and outline its advantages over a gene expression assay. Define Systems biology. 13 College Closed-No lecture 14 TBA/Review 15 Final Exam 25% **the order or content of some material is subject to change based on scheduling of guest speakers and/or site visit 1/14/2009 THIS COURSE ADHERES TO ALL COLLEGE POLICIES (See College Calendar) Page 6
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