Genomics and Gene Recognition Genes and Blue Genes

Transcription

1 Genomics and Gene Recognition Genes and Blue Genes November 3, 2004

2 Eukaryotic Gene Structure eukaryotic genomes are considerably more complex than those of prokaryotes eukaryotic cells have organelles a variety of chemical environments can exist within a cell each cell type typically has a distinct pattern of gene expression (even though the same DNA) there is a significant portion of introns and intergenic space whose role is mostly unknown eukaryotic cells (nuclei) almost always contain two copies of chromosomes animal cell

3 Chromosome Structure a very long, continuous piece of DNA contains many genes, regulatory elements and other intervening nucleotide sequences the uncondensed DNA exists in a quasiordered structure inside the nucleus it wraps around histones (structural proteins) this composite material is called chromatin sheer size and diversity of regulation and functions make eukaryotic DNA very hard to annotate (1) Chromatid (2) Centromere (3) Short arm (4) Long arm.

4 Eukaryotic Genomes

5 Transcription in Eukaryotes much more complex than in prokaryotes a typical mammalian cell has 1,500 times as much DNA than the cell of E. Coli DNA wrapped around histones which limits access of transcription regulatory proteins to promoters eukaryotic transcription requires factors that can recognize the chromatin so that the transcription machinery can access promoters

6 What is Transcription Factor? transcription factor is a complex of about 10 proteins transcriptional regulation coordinates metabolic activity, cell division, embryonic development transcription start is enabled by promoters enhancers response elements

7 Promoters promoters of eukaryotic genes that encode proteins are defined by modules of short conserved sequences (e.g. TATA box, CAAT box, GC box) CAAT box is usually located around position 80 GC box usually contains sequence GGGCGG or its complement GC box is usually found upstream of housekeeping genes genes that encode proteins commonly present in all cells and essential to normal function (they are expressed at relatively stable level in all cells) sets of various sequence modules are embedded in the upstream region of genes they collectively define the promoter every (almost) eukaryotic gene has its own promoter RNA polymerase II is responsible for the transcription of the protein coding genes

8 Promoters

9 Enhancers also called upstream activation sequences, or UASs enhancers are additional regulatory sequences and they assist transcription initiation differ from promoters location of enhancers is not fixed they may be several thousand nucleotides away from the promoter sometimes downstream from the gene bidirectional sequences function in either orientation can be removed and then reinserted in a different orientation without loss of function enhancers are also evolutionarily conserved enhancers are promiscuous stimulate transcription from any nearby promoter enhancer recognition depends on transcription factors

10 Promoters and Enhancers

11 Promoter Consensus Sequences

12 Response Elements response elements are promoter modules in genes responsive to common regulation found in the promoter regions of genes whose transcription is activated in response to a sudden increase in environment temperature -> heat shock proteins toxic heavy metals -> metal response elements heat shock element sequences are recognized by a specific transcription factor (HSTF) located at about +15 from the transcription start site of genes whose expression is dramatically enhanced consensus sequence for HSE is about 14bp long and it can be in introns too

13 Regulatory Influences many genes are subject to a multiplicity of regulatory influences this is achieved via an array of regulatory elements

14 RNA Polymerases there are 3 RNA polymerases in eukaryotic proteins RNA polymerases I and II are involved in transcribing RNA molecules RNA polymerase II transcribes protein coding genes RNA polymerase II DOES NOT directly recognize promoters this task is carried out by transcription factors (e.g. TATA-binding proteins) there are at least 12 TATA associated factors that bind to the nucleotide sequence in specific order transcription initiation site starts with an initiator sequence typically about 6 nucleotides long subtle differences in transcription factors are known to exist among different cell types

15 RNA Polymerases

16 Transcription Factors majority of transcription factors are sequencespecific DNA-binding proteins recognize consensus sequences, e.g. TATA box recognize enhancers

17 DNA Looping because transcription must respond to a variety of regulatory signals, multiple proteins are essential for appropriate regulation of gene expression these regulatory proteins are the sensors of cellular circumstances how do they work? they communicate this information by binding at specific nucleotide sequences DNA is a linear molecule so there is little space for all these proteins to bind all these sites are near transcription initiation site DNA looping enables additional proteins to interact with RNA polymerase II initiation complex DNA loping expands the repertoire of transcriptional regulation mechanism

18 DNA Looping

19 Post-Transcriptional Modification of mrna transcription and translation are separated in eukaryotes transcription occurs on DNA in the nucleus translation occurs on ribosomes in the cytoplasm transcript must move from nucleus into cytoplasm on its way, pre-mrna undergoes processing this primary transcript (hnrna) is converted into mature mrna each mrna encodes ONLY ONE protein (monocistronic RNAs) in prokaryotes, some are polycistronic

20 Post-Transcriptional Processing of mrna prior to processing hnrnas are capped and poly-adenylated Capping a set of chemical alterations at the 5 end of all hnrnas Poly-adenylation the process of replacing the 3 end of an hnrna with approximately 250 A s that are NOT spelled out in the nucleotide sequence of a gene exception: histones lack poly-a tail Splicing removal of often large segments from the interior of hnrna

21 Introns and Exons most genes in higher eukaryotes are split into coding and noncoding regions coding regions exons non-coding regions introns introns are removed from the primary transcript in the process called splicing trna and rrna also get spliced!!! Example: yeast actin gene has only one intron 309bp long, after the 3 rd amino acid chicken ovalbumin gene has 8 exons and 7 introns

22 Introns and Exons mosaic molecules consisting of sequences complementary to several non-contiguous segments of the viral genome Quote from: Adenovirus amazes at Cold Spring Harbor (1977) Nature 268: The notion of the cistron, the genetic unit of function that one thought corresponded to a polypeptide chain, now must be replaced by that of a transcription unit containing regions which will be lost from the mature messenger -- which I suggest we call introns (for intragenic regions) -- alternating with regions which will be expressed -- exons. The gene is a mosaic: expressed sequences held in a matrix of silent DNA, an intronic matrix. Gilbert, W. (1978) Why genes in pieces? Nature 271: 501

23 Open Reading Frames (ORFs) predicting genes is more difficult than in prokaryotes splice sites are hard to predict detecting sufficiently long ORFs is not enough to detect a gene alternative splicing even further complicates the issue ORFs would be useful in eukaryotes ONLY if we had algorithms that could accurately predict splice sites splice sites are very hard to predict, they are tissue specific there are at least 8 different splice signals GU-AG rule is the most common introns are at least 60bp long (to be able to accommodate splicing) introns can be tens of thousands of nucleotides long exons vary in length between about 100 and 2,000bp

24 Introns and Exons

25 Introns and Exons

26 Alternative Splicing majority of eukaryotic genes appear to be processed into a single mrna, but % of human genes give rise to to more than one mrna sequence how? via alternative splicing alternative splicing depends on a cell type and environmental circumstances splicing apparatus itself is made from a variety of snrnas and several proteins variations in splice junctions may reflect specific recognition

27 Alternative Splicing

28 GC Content in Eukaryotic Genomes overall, GC content does not vary as widely as in prokaryotes however, there is a large-scale variation of GC content within eukaryotic genomes it is very important for gene recognition algorithms eukaryotic ORFs are much harder to recognize there is a useful correlation between genes, upstream promoter regions, codon choices, gene length, gene density and GC regions are involved GC rich regions are termed CpG islands and they are very underrepresented as compared to other dinucleotides within DNA sequences CpG islands occur frequently at the 5 ends of genes (-1,500 to +500) with the level of GC content as predicted by chance

29 CpG Islands

30 CpG Islands

31 CpG Islands analysis shows ~45,000 of CpG islands about half of these islands are housekeeping genes many remaining CpG islands are associated with promoters of tissue specific genes CpG islands are rarely found in gene-free regions the reasons are chemical modifications of CpG s into CpA s and TpG s transcription requires un-methylated DNA methylation and acetylation of histones help process of transcription histones lose affinity to bind DNA and thus the chromatin becomes less tightly packed the areas become more accessible to RNA polymerases

32 Codon Usage Bias every organism prefers to use some triplets over others (to code for the same amino acid) Example in yeast Arg is frequently encoded by AGA (48%) although there are four other codons (CGC, CGA, CGG, AGG) fruit flies use CGA in 33% of the cases How do they occur consequence of the abundance of trnas within the organism consequence of the avoiding of stop codons

33 Transposons insertion sequences; jumping genes mobile genetic material that can be moved from one location of a gene and be inserted at another the movement occurs due to the presence of an enzyme which is encoded within transposon itself transposase enzyme coded by one or two genes it catalyses its transposition from one part of the genome to another the enzyme genes are surrounded by repeat segments transposition conservative the number of copies of the repeat does not change replicative copy number increases transposons are more common in bacteria, but are known to exist in eukaryotes as well (~1,000 transposons in human genome)

34 Repetitive Elements many DNA regions contain repetitive sequences typically, large repetitive chunks are divided into tandemly repeated DNA repeats that are interspersed throughout the genome tandemly repeated DNA satellites minisatellites and/or microsatellites Example: 5 CTCTCTCTCT 3 sequence in which the repeat unit is CT 5 ATTCGATTCGATTCG 3 sequence; the repeat unit is ATTCG

35 Tandem Repeats Satellite DNA long, simple sequences (up to 10mbp) with skewed nucleotide compositions repeating fragments of up to 2,000bp Minisatellite DNA not so long as satellites (up to 20kbp) copies of sequences of up to 25bp Microsatellite DNA shorter than minisatellites (up to 150bp) up to 100 copies of sequences of up to 5bp (typically 2-3) TAGTAGTAGTAGTAGTAGTAG..." Example: humans, CA repeats occur once every 10,000bp make 0.5% of human genome

36 Interspersed Repeats scattered randomly throughout genomes propagated by the synthesis of an RNA intermediate - process called retrotransposition there are three steps in retrotransposition an RNA copy of the transposon is transcribed by RNA polymerase (regular transcription step) RNA copy is converted into a DNA molecule by reverse transcriptase reverse transcriptase inserts the DNA copy somewhere else in the genome reverse transcriptase may be acquired through viral infections

37 Eukaryotic Gene Density very small in the human genome: 3% of DNA codes for genes 27% of DNA are promoters, introns, and pseudogenes 70% of DNA??? often called junk DNA unique sequences repetitive sequences genes are far apart the average distance between genes is about 65,000bp in E. Coli the average distance between genes is about 120bp