PCR & Molecular Biology

Transcription

1 PCR & Molecular Biology Certified High-Quality Products for PCR, Molecular Biology and Research Liquid Handling For over years

2 SARSTEDT International Your Partner in medicine and science worldwide Table of Contents Sarstedt quality symbols A warranty of reliability and reproducibility pp. 4 Biosphere plus Purity Certificate p. 4 PCR Performance Tested Purity Certificate p. 4 Pipette tips Crucial for precise dosing pp Aerosol filters Protection against carry-over contamination p. 5 Quality guarantee by conformity testing at regular intervals p. 6 Superior shielding with the special ShaftProtect 10μl tip p. 7 Filter tips Pipetting small volumes from μl pp. 8-9 Filter tips Pipetting sample volumes from 1 1,250μl pp Tip SystemBox - Central pipetting station and reload trays p. 12 Tip StackPack / Starter Kit the economic reload system p.13 Ordering information on quality pipette tips with and without filter pp Compatibility Table pp For over years Robotic tips Tips for Biomek pipetting platforms p. 20 Multiply reaction tubes PCR tubes, strips of 8 tubes pp Product description of Multiply PCR tubes, strips and lids p. 21 Ordering Information for Multiply PCR tubes, strips and lids p. 22 Accessories: The interchangeable RackSystem for sample preparation p. 23 Multiply PCR plates White and transparent options in 96/384 well format pp A B C D E F G H White PCR plates optimized for qpcr pp Ordering information for white PCR plates and optical lids p. 26 Transparent PCR plates, w/o skirt, with half and full skirt pp Ordering information for transparent PCR plates and lids p. 29 Compatibility Table pp Deep well MegaBlock Free from DNA, DNase/RNase and endotoxins p. 32 Adhesive sealing tapes For qpcr/pcr, automation and sample storage p Highly transparent, adhesive qpcr tape p. 33 Transparent tape for end point PCR/storage, strongly adhesive p. 34 Aluminum tape for PCR and sample storage p. 34 Pierceable tape for DNA sequencers, HPLC, autosamplers p. 35 SafeSeal micro tubes 0.5/1.5 and 2.0 ml with secure cap locking feature pp UV transparent single-use cuvettes For photometric analyses 220 nm pp

3 Sarstedt quality symbols for laboratory products High purity Biosphere plus filter tips for pipetting Clean room conditions, skilled personnel in protective clothing as well as automated processes form the basic prerequisites for award of the certified Sarstedt quality standards, PCR Performance Tested and Biosphere plus. Since 1995, we have provided our customers with a product quality tailored to their specific applications to ensure utmost reproducibility in their analyses. Our certification standards are continually adjusted to research requirements providing reliable performance in routine work at any time. Protection against carry-over and contamination Even the most meticulous pipetting may generate aerosols during aspiration of the sample, contaminating the pipette and any sample material to follow. This cross-contamination cannot be tolerated when working with expensive, hazardous, or irreplaceable samples or when using highly sensitive methods. In order to prevent aerosol contamination along with the resulting consequences (falsified results, additional expenditure in time and cost) Sarstedt incorporates special porous elements in its range of filter tips. PCR Performance Tested quality Sarstedt s quality and purity standard PCR Performance Tested has been created to accommodate the specific requirements and needs involved in working with nucleic acids (e.g. qpcr, PCR, purification and storage). Thanks to this quality grade, customers can use Sarstedt disposables directly in nucleic acid analytics. PCR Performance Tested products meet the following purity criteria that are certified by an independent laboratory: DNA-free DNase-/RNase-free PCR inhibitor-free Reliability with selected filter materials The filter element integrated in the pipette tips consists of a porous plastic material featuring defined hydrophobic properties and no self-sealing additives. The sponge-like material is densely composed of randomly arranged channels (see picture on left), enabling air to pass for precision when using air displacement pipettes. Simultaneously, the complex structure of the porous filter prevents aerosols from entering the pipette shaft. We guarantee compliance with the following threshold values: Human DNA <0.5 pg/μl Bacterial DNA <0.02 pg/μl DNase <1x10-5 U/μl RNase <1x10-9 Kunitz units/μl Biosphere plus quality - the Plus in safety In recent years, scientific analysis methods have been continuously refined so that individual molecules can be identified. As sensitivity increases, disposables must meet even more sophisticated requirements so that users can be sure to reliably exclude even the smallest contamination. The new Biosphere plus quality constitutes a purity standard that enables utmost protection against any possible contamination. In addition to our high-purity production conditions, all products certified Biosphere plus are subjected to a validated decontamination process to ensure values significantly lower than the thresholds guaranteed for the PCR Performance Tested quality. Furthermore, Biosphere plus products are ATP- and pyrogen-free as well as sterile in accordance with ISO Biosphere plus products meet the following purity criteria that are certified by an independent laboratory: Sterile DNA-free DNase-/RNase-free PCR Inhibitor-free ATP-free Pyrogen-free We guarantee compliance with the following threshold values: Human DNA <5.0 fg/μl Bacterial DNA <0.2 fg/μl DNase <5x10-7 U/μl RNase <5x10-11 Kunitz units/μl ATP <1x10-12 mmol/μl Pyrogens < EU/ml Sterility validated according to ISO Technical modifications reserved Technical modifications reserved Cross section of a porous filter element (40-fold magnification) piston pipette shaft sealing surface of pipette tip aerosol sample aerosol-free filter Measuring the efficiency of filter inserts in Biosphere plus filter tips* Both the PCR-based method and indirect, radioactive measurement of the plasmid DNA with P-32 marked ATP are reliable methods for detecting carry-over contamination of DNA aerosols likely to occur in practical pipetting applications. With both methods, the hydrophobic filter inserts with the selected porosity used in Sarstedt Biosphere plus filter tips were found to provide protection against aerosol contamination. The superior efficiency of the filter in Biosphere plus filter tips is based on a complex mechanism of absorption, surface quality, thickness, strength and shape. * Refer to our Application Report Shielding Effect of Filter Inserts in Pipette Tips for additional details Added protection with Biosphere plus purity quality Maximum biological purity: free from DNA, DNase, RNase, PCR inhibitors, ATP and pyrogens Batch-specific certification is available upon request Convenient and aseptic packaging of individual tip boxes Each tip box is additionally and aseptically protected in a special sterile bag packaging Easy opening of bag packaging without aid via notch at short bag side (see picture) 4 5

4 The pipette tip Crucial for precise dosing A calibrated system: Pipette and pipette tips The precision of the pipetting process is primarily determined by the optimal compatibility of the pipette and pipette tip. One of the leading manufacturers of pipette tips, Sarstedt s main objective for years has been to provide customers with tips in optimal, reliable quality to fit most marketable pipette brands. The quality of a tip is defined by the properties of the material used, the design and optimum fit on the individual pipettes. Quality guarantee: Precision and accuracy for small volumes In particular, the ongoing trend towards ever decreasing small sample volumes inevitably involves increasing demands on the dosing system. At regular intervals, Sarstedt tips are checked for maximum precision and reliability in combination with the pipettes of leading manufacturers (e.g. Eppendorf, Gilson). In addition, the tips are subjected to a defined procedure of comprehensive in-process conformity testing in accordance with the international standard ISO 8655 for piston pipettes. Note on availability of a Declaration of Conformity for pipette/pipette tip combinations Precise pipetting of micro volumes - superior precision and contamination protection with the ShaftProtect 10 μl tip for Gilson P2/P10 With most extra long tips the dead volume is extended, which creates adverse effects on performance - but ShaftProtect has been especially developed for Gilson P2/P10 pipettors. ShaftProtect 10μl 2μl ShaftProtect 10 μl tip Filter tip μl Extra-long tip with ShaftProtect for Gilson P2/P10 to protect the inner tube wall against the generally non-sterile pipette shaft Identical precision and accuracy thanks to a very small dead volume comparable to the short tip Enhanced dosing features Especially fine, extra-long 'ShaftProtect' 10 μl tip for easy dosing of even the smallest volumes Easy visual control Calibration rings facilitate visual control for micro volumes Conformity-tested to ISO 8655 Protected, precise and accurate pipetting down to the base of 1.5 / 2.0 ml micro tubes with the ShaftProtect 10 μl tip for Gilson P2/P10 The Declaration of Conformity for piston pipettes indicates the combination of pipette and pipette tip to which it applies attesting precision and reliability and can be made available on request. The logo on the label indicates that a standard Declaration of Conformity is available for a particular pipette tip. Flexibility & convenient handling With the dual position tip-ejector* (see illustration), the extended ShaftProtect tip covers as much of the pipette shaft as the short Dual position tip-ejector twisted by 180 (Pos. 1/Pos. 2) in axial direction allowing choose between the long and short tips * All new (since 2003) P2/P10 pipettors from Gilson are equipped with dual position tip-ejector, allowing the use of short tip xxx and Gilson long xxx. Older P2/P10 models need the Gilson Service Kit 2. ShaftProtect 10μl tip sterile non-sterile Standard 10μl tip Dual position tip-ejector Pos Pipette cone sealing position Dual position tip-ejector Pos

5 The pipette tip Crucial for precise dosing Pipetting small sample volumes μl Working routinely with micro volumes can adversely affect the pipette / pipette tip dosing system. Therefore, tips to 10 μl feature the following product benefits of enhanced visual control and dosing: Enhanced pipetting safety due to calibrated system - conformity-tested to ISO 8655 Easy visual control for micro volumes - calibration rings facilitate visual control of the pipetted volume Enhanced dosing features - fine, thin tip facilitates dosing and visual volume control Pipetting micro volumes from specially designed tip for perfect fit on Eppendorf pipettes Tip with ergonomic and flexible cone, enhanced sealing properties and outstanding precision on Eppendorf pipettes. The cone geometry offers exceptional fit and effortless dosing with new generation single- or multi-channel pipettes (e.g. Research plus pipettes). The tip is also compatible with identical pipette models from other manufacturers. Filter tip Compatible with μl Eppendorf Research, Research plus, Research pro, Xplorer, Reference 2 - and Finnpipette Digital μl, as well as models of identical design / μl 2 μl Filter tip / μl / μl Mini Gilson tip for Gilson P2/P10 or Eppendorf Research 2.5 μl with finely drawn tip and calibration rings in 2 and 10 μl tips, as well as for versions of identical design from Finnpipette, Biohit and Socorex. Conformity tested to ISO μl 10μl 2μl 46 mm tip length provides protected pipetting down to the bottom of deep vials Cone geometry optimized for 8- and 12-channel pipettes Graduation rings without interfering ridges for easy dosing into vials Extra thin tip for enhanced dosing of micro volumes Visual control of micro volumes Graduation rings at 2, 10 and Conformity tested to ISO μl 2 μl Filter tip * μl Universally compatible with Gilson, Eppendorf, or identical designs, e.g. Biohit, Finnpipette, Abimed and Brand for volumes between 0.1 and 10 μl. The fine, extra-long tip and calibration rings facilitate dosing and visual volume control. * Compatible with Eppendorf Reference 2 - and identical pipette models from other manufacturers Filter tip μl Type crystal tip for Eppendorf μl, Reference/Research or identical designs, e.g. Finnpipette. Conformity tested to ISO 8655 Conformity tested to ISO

6 The pipette tip Crucial to precise dosing Pipetting sample volumes μl Pipetting large sample volumes up to 1,250 μl /.213 Filter tip / μl Standard pipette tip for pipettes from Eppendorf, Gilson, Biohit, Brand, Socorex as well as identical designs, and additional filter tip option of volume ( ). Two pipette tips with graduation rings for volumes between 1 and 1,250 μl Saves space as a large volume range is covered with 2 tips only Color-coded trays enable easy volume identification Highly transparent tip material for enhanced pipetting control Conformity tested to ISO 8655 Filter tip μl Standard pipette tip for pipettes from Eppendorf, Gilson, Biohit, Finnpipette, Socorex and identical designs μl 100 μl 50 μl 10 μl Filter tip μl, extra long Extra long and slim 200 μl tip for 20, 50, 100 and 200 μl pipettes. Designed for universal use on a pipette volume range between 2 and 200 μl: Eppendorf Reference, Research, Biohit mline, -Proline, -Proline Electronic, HTL, Labnet, Finnpipette, Nichiryo Benchmate, Gilson, Rainin, CappAero, CappTronic Electronic and identical designs. - Soft, flexible cone for easy assembly and ejection - 77 mm length for reaching into tubes - One pipette accommodates 4 different volumes - Graduation rings without interfering ridges at 10, 50, 100 and 200 μl for visual control of the defined pipetting volume Conformity tested to ISO Filter tip ,000 μl Standard tip for Eppendorf, Gilson, Finnpipette, Biohit, Brand, Socorex and identical designs. - Empty box without pipette tips , 10 pcs./case - Graduation rings at 100, 500 and 1,000 μl Conformity tested to ISO Filter tip μl 300 μl 200 μl 100 μl 50 μl Specially suited for multi-channel pipettes, e.g. from Eppendorf, Brand, Finnpipette, Biohit. Also compatible with older multi-channel pipettes up to 200 μl mm tip with 4 graduations for visual control of pre-set pipetting volume at 50, 100, 200 and 300 μl. Conformity tested to ISO ,000 μl 500 μl 200 μl Filter tip ,250 μl, extra long Extra long (103 mm) and slim pipette tip, universally suited for use with single-channel pipettes, e.g. from Gilson, Biohit, Nichyrio and Finnpipette. Particularly recommended for applications in Deep Well blocks, Cluster tubes or 5 ml CryoPure tubes for which the traditional 1,000 μl tip is too short or too thick. Compatible with multi-channel pipette Finnpipette Novus 100 to 1,200 μl. 3 Graduations for visual control of pre-set pipetting volume at 200, 500 and 1,000 μl. - Empty box without pipette tips , 8 pcs./case 10 11

7 Tip SystemBox - Methodical tip processing The Tip SystemBox: Central Pipetting Station Flexible and cost-effective Simple and practical Tip StackPack reload system Tips are preloaded in stacked trays of 96 tips each. The base section and cover are designed for multiple use in combination with the 5 or 6 tip trays. As a result, composite materials and storage space are reduced by more than 50%. Space-saving Economic and environmentally friendly Easy to use Reduced waste volumes Tip StackPack Starter Kit (3 x Tip StackPack + 1 Tip SystemBox) Ordering Information for Tip StackPack see page 15 Empty 96-tip refill trays and bulk tips Suitable for manually racking bulk tips for autoclaving and convenient handling.* Empty reload tray for use in the Tip SystemBox Users purchasing tips from our range loose in bags can load tips into empty trays (grey, yellow, orange) in the Tip SystemBox. Place empty trays into the Tip SystemBox and load with tips. Autoclave tips in the Tip SystemBox, if required.* Ordering Information Empty Reload Trays Colored trays for easy tip volume identification Tip StackPack Efficient handling of 96-tip trays, reduced packaging and storage space Order No. Tray color code Tip type** / Volume (pcs./case) Economic and environmentally friendly Easy transfer of individual layers of 96-tip trays into the Tip SystemBox for easy handling or autoclaving* Safety labels applied on both sides can be easily perforated to access the next layer of tips Trays can be placed into the Tip SystemBox without further adjustment Tips can also be used directly from the stack Ordering Information Tip SystemBox Order No Description Tip SystemBox, empty (without Pipette tips) pcs./case 6 Safety labels applied on both sides can be easily perforated to access the next layer of 96 tips A, C / 200 μl D, J, N / E long / 10 μl 25 Tip StackPack Starter Kit The Tip StackPack Starter Kit contains 3 Tip StackPacks and 1 Tip SystemBox each Ordering Information Tip StackPack Starter Kit Order No. Volume Pipette tip color /case E / 10 μl L / 300 μl 20 * Important notes on autoclaving: See page 20 ** Pipette type see pages 18 to μl μl yellow μl * Important notes on autoclaving: See page 20 3 x Tip StackPack + 1 x Tip SystemBox 12 13

8 Ordering Information - Quality tips with filter Ordering Information - Quality tips without filter Filter tips (illustrations 1:1) Order No. Max. Sample Volume/ Color Quality Grade Tips without filter (illustrations 1:1) Order No. Max. Sample Volume/ Color Quality Grade Type E Type E μl, μl, μl, Type E long μl, Type D μl, μl, 96/box 480/inner box 96/box 480/inner box 480/bag (loose) 4,800/case 96/box 480/inner box 96/box 480/inner box 480/bag (loose) 4,800/case Type N , 480/inner box Type J Type A Type A , , , , μl, μl, Type O extra long μl, 480/bag (loose) 4,800/case 96/box 480/inner box 96/box 480/inner box 480/bag (loose) 4,800/case 96/box 480/inner box 480/bag (loose) 4,800/case 96/box 960/case Type C μl, 480/inner box Type L Type B μl, μl, ,000 μl, ,000 μl, Type M 1,250 μl, ,250 μl, 480/bag (loose) 4,800/case 96/box 480/inner box 100/box 500/inner box 1,000/case 200/bag (loose) 2,000/case 96/box 768/case 384/bag (loose) 3,840/case Type E ,000/bag, 10,000/case /box, μl, 96/box, 480/inner case, Tip StackPack /tray, 576/StackPack, 2,304/case Tip StackPack Starter Kit Type E long μl, 500/bag, 10,000/case /box, Type D ,000/bag, 10,000/case , 96/box, /box, 480/inner case, Type N ,000/bag, 10,000/case , 96/box, /box, 480/inner case, Type J ,000/bag, 10,000/case , 96/box, /box, 480/inner case, Type A /bag, 10,000/case /box, μl, 96/box, 480/inner case, Tip StackPack /tray, 480/StackPack, Tip StackPack Starter Kit Type O extra long μl, 96/box, 960/case Type C /bag, 10,000/case /box, 480/inner case, 250 μl, Tip StackPack 96/tray, 480/StackPack, Tip StackPack Starter Kit Type L /bag, 5,000/case μl, 96/box, /box, 480/inner case, Type B /bag, 5,000/case ,000 μl, 100/box, 1,000/case /box, 500/inner case, 1,000/case Type M /bag, 3,840/case ,250 μl, 96/box, 3,840/case /box, 768/case Scale 1:2 Scale 1:2 Scale 1: /bag, 2,500/case 5 ml /Box, 500/case /bag, 2,500/case 5 ml /box, 500/case /bag, 1,000/case 10 ml /box, 250/case Refer to pp. 16 to 19 or see Catalog for further information 14 15

9 Compatibility Table - Pipette Tips Eppendorf Pipettes Thermo Finnpipettes Gilson Pipettes Eppendorf Reference Eppendorf Research Eppendorf Research plus Eppendorf Research pro Xplorer Finnpipette Digital / F1 / F2 Finnpipette Focus Finnpipette Novus Bio Control Pipetman / Ultra / Concept Certified pipette tips with filter (illustrations 60%) μl μl μl μl μl μl μl μl μl MC 500-5,000 μl 1-10 ml μl μl MC μl MC μl μl MC 500-5,000 μl 1-10 ml μl μl MC μl μl MC μl μl MC 50-1,000 μl 50-1,200 μl MC 100-5,000 μl μl MC μl F μl F μl μl Univ μl MC 2- Univ μl MC μl Univ μl Univ μl MC μl μl μl 1-10 μl μl μl μl MC 100-1,200 μl MC μl MC P2/U μl P10/U μl C8/C μl P20/U20 2- P100/U μl P200/U μl U μl P1.000/U ,000 μl P5.000/U ,000 μl P10/U ml 2.5 μl μl μl 10 μl 10 μl 300 μl 200 μl 200 μl ,000 μl 1,250 μl μl 10 μl 200 μl Quality pipette tips without filter* (illustrations 60%) Eppendorf Reference Eppendorf Research Eppendorf Research plus Eppendorf Research pro Xplorer Finnpipette Digital / F1 / F2 Finnpipette Focus Finnpipette Novus Bio Control Pipetman / Ultra / Concept μl ,250 μl 1,000 μl 300 μl 250 μl ml Scale 1: ml Scale 1: ml Scale 1: Legend: 10 = max. pipetting volume = limited pipetting volume = not recommended MC = not checked = multi-channel pipette Pipette suppliers occasionally rework or carry out technical modifications to the pipette cones of their pipettors so that the information provided in the Compatibility Table constitutes a recommendation only. In case of doubt, free sample tips are available on request to test the optimum fitting and ejection behavior of our tips when used on your pipettes. * Important notes on autoclaving see page

10 Compatibility Table - Pipette Tips Biohit Pipettes Brand Pipettes Socorex Manual / Electronic Pipettes mline Mechanical Proline Mechanical eline Electronic Transferpette Digital Transferpette S Transferpette Electronic Calibra Manual Micro 822 C. M. Multi 852 Acura Manual Micro 825 Acura Electro Micro 925 A. 835/ 935e Acura Multi 855/955e Certified pipette tips with filter (illustrations 60%) μl μl MC μl μl μl MC 100-5,000 μl μl μl 5-50 μl 5-50 μl MC μl μl MC μl MC 100-5,000 μl μl μl 50-1,000 μl 50-1,200 μl MC 100-5,000 μl μl μl μl μl μl μl MC μl μl μl μl MC μl μl μl MC 1-10 ml μl μl MC 2-1- MC μl μl 1-10 μl 1-10 μl μl μl 1-10 μl μl μl μl μl 1-10 μl μl μl μl ml μl MC 5-50 μl MC μl MC 10 μl 10 μl 10 μl 2.5 μl μl μl μl μl ,000 μl 1,250 μl μl 10 μl 200 μl Quality pipette tips without filter* (illustrations 60%) mline Mechanical Proline Mechanical eline Electronic Transferpette Digital Transferpette S Transferpette Electronic Calibra Manual Micro 822 C. M. Multi Acura Manual Micro 825 Acura Electro Micro A. 835/ 935e Acura Multi 855/955e 200 μl ,250 μl 1,000 μ 300 μl 250 μl ml Scale 1: ml Scale 1: ml Scale 1: Legend: 10 = max. pipetting volume = limited pipetting volume = not recommended MC = not checked = multi-channel pipette Pipette suppliers occasionally rework or carry out technical modifications to the pipette cones of their pipettors so that the information provided in the Compatibility Table constitutes a recommendation only. In case of doubt, free sample tips are available on request to test the optimum fitting and ejection behavior of our tips when used on your pipettes. * Important notes on autoclaving see page

11 Pipettenspitzen tips for für Biomek Biomek pipetting Pipettierroboter platforms Reliable quality Multiply reaction tubes for PCR The quality solution for automated liquid handling Each production batch is tested for functionality and precision on original robotic instruments to ensure a consistently superior product performance All tips are certified DNase-, RNase- and human DNA-free Sterile tips are also tested to be non-pyrogenic The colored trays enable easy identification of tips and volumes Pipette tips for Biomek Product / Volume, tray Tip with filter, sterile Tip without filter, non-sterile Tip without filter, sterile Enhanced design features of the Multiply tubes ensure work is risk-free from contamination and non-tiring compared to traditional reaction tubes. Prevents accidental contact with the inner surface of the lid - Easy to open - Anti-contamination shield prevents potential contamination * ** ** 96/rack *4,800/case **9,600/case Frosted, flat lid with large writing area 35 μl 50 μl ** * ** 96/rack *4,800/case **9,600/case - The frosted surface prevents smearing even when processed in a thermocycler. - The space-saving lid design allows complete utilization of the heating block capacity. 175 μl 250 μl /rack 9,600/case 1,000 μl /rack 3,600/case Sample preparation/storage in the RackSystem pipetting station - Small recess near the top of the Multiply PCR tubes allows them to be easily removed from the work tray. - The small, roughened recess can also be used as an additional writing area. Pipette tips for Biomek FX (P) /NX (P) Description for RackSystem see page 23 Product / Volume, tray Tip with filter, sterile Tip without filter, non-sterile Tip without filter, sterile * * ** 96/rack *4,800/case **9,600/case product range: 35 μl 50 μl /rack 4,800/case - On request, we also offer conveniently packaged small quantities of products certified to the sterile Biosphere plus, or PCR Performance Tested quality standard - Variety of options - Optimum heat transfer thanks to thin-walled reaction space 175 μl 250 μl /rack 4,800/case - Compatible on thermocyclers with 0.2 and 0.5 ml block formats - Enables micro centrifugation in most commonly used micro centrifuges (with adapter, as required) Important notes on autoclaving: Products made from PP can be autoclaved up to 121 C without significantly impairing their mechanical properties. Users are to test for themselves if autoclaving may have any effect on other characteristic product features so as to influence the individual application concerned

12 Ordering Information Multiply PCR Tubes Accessories: Interchangeable RackSystem for 0.2 ml Multiply PCR tubes PCR single tubes Product Order No. Volume Description Sterile Color Biosphere plus Multiply -Pro 0.5 Biosphere plus sterile Quality Grade Pieces per bag 100 pieces 500 pcs./inner case 1,000 pcs./case Safe and fast handling of Multiply tubes Flexible, two-piece system comprising a work tray and support base Easily inserted in the thermocycler, no need to remove tubes Convenient 96 well microtiter format to fit any processing system Stackable design provides space-saving specimen storage ml pieces 2,000 pcs./case Multiply -Pro 0.5 mixed colors pieces 3,000 pcs./case ml Biosphere plus Multiply -Pro 0.2 Biosphere plus sterile 250 pieces 1,000 pcs./inner case 2,000 pcs./case Multiply -Pro pieces 2,000 pcs./case Flexible, compact work station for sample preparation and space-saving specimen storage Suitable for all Multiply tubes PCR tube 8-strips with and without lid Product Order No. Volume Description Sterile Color Multiply -μstrippro, optically clear flat cap Multiply -μstrippro, optically clear flat cap, low profile Quality Grade Strips per bag strips 1,200 strips/case strips 1,200 strips/case Sample preparation and PCR applications ml Multiply -μstrippro, dome cap strips 1,200 strips/case mixed colors 120 strips 1,200 strips/case Assembly 0.2 ml Multiply tube loading......or, alternatively, Multiply PCR plate loading. Ice-bath pipetting Specimen centrifugation in completely assembled RackSystem......fast and easy placement of specimens into the thermocycler Multiply -μstrip strips 480 strips/case mixed colors 120 strips 480 strips/case Position empty PCR work tray to rest securely on the support base. Align single tubes to 45 for easy handling. The PCR plate comprises 96 tubes in 12 strips of 8 tubes each. RackSystem can be used as a pipetting station. Compatible with any microtiter tray rotor. After amplification, reposition PCR tray onto stackable support base for space-saving storage. Lid strips for Multiply -μstrip and Multiply PCR plate Product Order No. Description Compatible with Sterile Color Quality Grade Strips per bag Ordering Information PCR RackSystem Illustration Order No. Product Description Description Multiply -μstrip Biosphere plus sterile ( / 12 strips 240 strips/case strips of 8 lids, ) flat, and highly transparent, for real-time PCR Multiply PCR plate (72.985) strips 480 strips/case PCR work tray for 0.2 ml PCR tubes for 96 tubes red Support base for PCR work tray blue 5/bag 5/case green yellow 22 23

13 A B 1 C 2 D E 3 F 4 G H A B C D E F G H White PCR plates for quantitative PCR Multiply PCR plates made from polypropylene, white White wells for improved fluorescence reflection Certified PCR Performance Tested Thin-walled reaction tubes for quick temperature transfer Fits thermocyclers processing 96 and 384 well block formats Barcode labeling on plates with half or full skirt is available on request 96 Well Multiply PCR plate, without skirt level - NEW: Easy to cut - Raised well rims, optimized for Heat-Sealing films - Fits most commonly used thermocyclers - Can be sealed with qpcr film (see p. 33) or optically clear strips of 8 lids (see p. 26) Cost-effective miniaturization of qpcr assay in white Multiply PCR plates Fluorescence-based applications like qpcr, particularly those involving small volumes, benefit from the significantly improved reflection characteristics of the white wells. Specimens of low DNA concentration, too, can be more easily detected (see Fig. 1). Compared to transparent wells, a 10-fold fluorescence level can be measured with white wells. Thanks to maximum signal reflection, C t values are obtained earlier (see Fig. 2). In white plates sealed with qpcr compatible tapes, the reagent volume (SYBR Green) per well can be reduced from 25 μl to 15 μl without significant changes in sensitivity and stability, enabling a 40% decrease in qpcr reagent costs. In addition, the white plates minimize adverse effects like cross talk that would increase well-to-well variability in transparent wells. 96 Well Multiply PCR plate with half skirt, flat deck - Recommended for Applied Biosystems (ABI) thermocyclers and DNA sequencers - Flat deck enables safe sealing with tapes - Raised well rims, optimized for Heat-Sealing films - Can be sealed with qpcr film (see p. 33) or optically clear strips of 8 lids (see p. 26) C t values for the detection of plasmid copies in white and transparent plates at reduced reaction volumes Increased sensitivity due to maximum signal reflection C t values ,000, ,000 10,000 1, Fig. 1 Number of plasmid copies C t at 15 μl (white) C t at 25 μl (transparent) Fluorescence Fig. 2 Cycles 96 Well Multiply PCR plate with full skirt, low profile well design - Enhanced stability for automated systems - Suitable for real-time systems - Raised well rims, optimized for Heat-Sealing films - Can be sealed with qpcr film (see p. 33) or optically clear strips of 8 lids (see p. 26)

14 A B C D E F G H A B 1 C 2 D E 3 F 4 G H White PCR plates for quantitative PCR Quality in series 96 and 384 Well Multiply PCR plates Lightcycler 480 PCR plate with 96 wells Multiply PCR plates made from polypropylene - Raised well rims, optimized for Heat-Sealing films - Can be sealed with qpcr film (see p. 33) or optically clear strips of 8 lids (see below) Certified 'PCR Performance Tested' Quick temperature transfer due to thin-walled reaction tubes Fits thermocyclers processing 96 and 384 well block formats Raised well rims, optimized for Heat-Sealing films Plates with half and full skirt; Barcode available on request Well Multiply PCR plate, without skirt, pre-assembled with strips of 8 tubes Lightcycler 480 PCR plate with 384 wells - Max. volume: 30 μl per well - Raised well rims, optimized for Heat-Sealing films - Can be sealed with qpcr film (see p. 33) - Available in the Biosphere plus quality, individually wrapped sterile - Suitable for real-time PCR - Can be sealed with optically clear strips of 8 lids (order no , see p. 29) - Tray from polycarbonate - Suitable for use in RackSystem, see p Well Multiply PCR plate, without skirt White Multiply PCR plates and lid strips for 96 Well plates Product Order No. Special feature Description Quality Grade Color - Easy to cut - Raised well rims, optimized for Heat-Sealing films - Fits most commonly used thermocyclers - Can be sealed with optically clear strips of 8 lids (see p. 29) - Can be sealed with qpcr films (see pp ) Optimized for qpcr 96 Well, without skirt white 50 pcs./inner case 200 pcs./case Optimized for qpcr 96 Well, with half skirt and low profile white 25 pcs./inner case 100 pcs./case Optimized for qpcr 96 Well, with full skirt, low profile for Fast PCR white 50 pcs./inner case 200 pcs./case 96 Well Multiply PCR plate with half skirt Optimized for qpcr Optimized for qpcr Optimized for qpcr ABI style, 96 Well, with half skirt, low profile for Fast PCR Lightcycler 480, 96 Well, with half skirt Lightcycler 480, 384 Well, with full skirt white white white 25 pcs./inner case 100 pcs./case 25 pcs./inner case 100 pcs./case 50 pcs./inner case 100 pcs./case - Recommended for Applied Biosystems (ABI) thermocyclers and DNA sequencers - Suitable for real-time PCR - Can be sealed with optically clear strips of 8 lids (see p. 29) - Can be sealed with qpcr films (see pp ) - Also available with barcode label ( ) Optimized for qpcr strips of 8 lids, flat for 96 Well plates, highly transparent 300 pcs./inner case 1,200 pcs./case 26 27

15 A B 1 C 2 D E 3 F 4 G H A B C D E F G H Quality in series 96 and 384 Well Multiply PCR plates Ordering Information Multiply PCR plates & lid strips 96 Well Multiply PCR plate with half skirt, with flat deck Multiply PCR plates, transparent - Recommended for Applied Biosystems (ABI) thermocyclers and DNA sequencers - Flat deck for easy and safe sealing of wells with tapes - Suitable for real-time PCR - Can be sealed with optically clear strips of 8 lids (see p. 29) - Can be sealed with qpcr films (see pp ) Product Order No. Well/Max. Volume / 0.3 ml Description Sterile Color Biosphere plus Multiply PCR plate without skirt, pre-assembled, raised wells Biosphere plus, sterile Quality Grade 1 pcs./bag 20 pcs./case / 0.3 ml Multiply PCR plate without skirt pcs./inner case 100 pcs./case 96 Well Multiply PCR plate with full skirt, 'low profile' well design - Enhanced stability for automated systems - Suitable for real-time PCR - Can be sealed with optically clear strips of 8 lids (see p. 29) - Can be sealed with qpcr films (see pp ) / 0.3 ml / 0.3 ml / 0.3 ml with barcode label Multiply PCR plate, with half skirt and flat deck Multiply PCR plate with half skirt Multiply PCR plate with half skirt pcs./inner case 100 pcs./case 25 pcs./inner case 100 pcs./case 25 pcs./inner case 100 pcs./case / 0.2 ml Multiply PCR plate with skirt 'low profile' pcs./inner case 200 pcs./case 96 Well Multiply PCR plate with half skirt, optimized for ABI Fast PCR System / 0.2 ml Multiply Fast PCR plate 'low profile' pcs./inner case 100 pcs./case - Low profile design reduces dead volume and cycle times - Suitable for real-time systems - Can be sealed with optically clear strips of 8 lids (see p. 29) - Can be sealed with qpcr films (see pp ) / 40 μl Lid strips for Multiply PCR plates Multiply PCR plate with skirt pcs./bag 50 pcs./case Product Order No. Description Compatible with Sterile Color Quality Grade Strips per bag 384 Well Multiply PCR plate with robust skirt - 40 μl maximum volume per well - 8 apertures in the frame facilitate positioning and removal of the plate from the heating block - Can be sealed with qpcr films (see pp ) strips of 8 lids, flat, highly transparent, for real-time PCR strips of 8 lids, flat, highly transparent, for real-time PCR Multiply PCR plate Multiply PCR plates Biosphere plus, sterile strips 12 strips 240 strips/case 300 strips/inner case 1,200 strips/case

16 A B 1 C 2 D E 3 F 4 G H A B 1 C 2 D E 3 F 4 G H Multiply PCR plates - Compatibility Table PCR plate range - Compatibility Table PCR plate range - Compatibility Table Model 96 Well without skirt, pre-assembled 96 Well without skirt 96 Well with half skirt, high profile 96 Well with full skirt, low profile 96 Well with half skirt, Fast PCR, low profile 384 Well with full skirt Model 96 Well without skirt, preassembled 96 Well without skirt 96 Well with half skirt, high profile 96 Well with full skirt, low profile 96 Well with half skirt, Fast PCR, low profile Lightcycler 480, 96 Well, full skirt 384 Well with full skirt Lightcycler 480, 384 Well, full skirt Order No. Standard PCR plates Amersham Bioscience MegaBace 1000 DNA Analysis System (old Model) MegaBace 1000 DNA Analysis System (new Model) MegaBace 4000 DNA Analysis System MegaBace 5000 DNA Analysis System Applied Biosystems ABI 2720 ABI Prism 7000, 7700 ABI Prism 7300, 7500, 7700 real-time PCR-System ABI Prism 7900HT real-time PCR-System ABI Prism 7500 Fast real-time PCR-System ABI Prism 7900 Fast real-time PCR-System ABI Prism 7900HT Fast real-time PCR-System ABI Prism 9800 Fast PCR-System GeneAmp 9600 GeneAmp 9700 GeneAmp 7500/5700 PE 2700 PE 9600 PE 9700 StepOnePlus Fast real-time PCR-System Seq. ABI Prism 310 Genetic Analyzer * Seq. ABI Prism 3100/3130 (XL) Genetic Analyzer * Seq. ABI 3500/3700/3730 (XL) DNA Analyzers * Veriti 96 Well / 384 Well Veriti Fast 96 Well Analytik Jena qtower 2.0/2.2 FlexCycler 96 Well FlexCycler 2 96 Well SpeedCycler 2 96 Well Biometra TOptical TRobot 96 Well TRobot 384 Well TProfessional Family 96 Well TProfessional Family 384 Well * with compatible adapter from ABI Order No. Standard PCR plates Bio-Rad/MJ Research CFX 96, real-time icycler, real-time iq5, real-time MyCycler Opticon 2 System, real-time / C1000 Serie / S1000 Serie PTC-200 PTC-225/220/221 PTC-100 Corbet Research / Qiagen Palm Cycler Eppendorf Mastercycler nexus Mastercycler ep realplex Mastercycler Gradient Mastercycler pro Ericom Deltacycler SingleBlock TwinBlock MWG Primus 96 Primus 384 The Q-Lifecycler Roche Lightcycler 96 Lightcycler 480 Stratagene / Agilent Technologies Gradient Temp. Cycler Mx3000/4000 Multiplex, QPCR System Robocycler 96/384 Techne Flexigene Genius OMN-E PCR Express Primus 96 Px2/PxE Quantica, real-time TC412/TC512 Touchgene Gradient Legend: = recommended = not recommended = not checked 30 31

17 96 Deep well MegaBlock Adhesive sealing tapes for qpcr/pcr, automation and sample storage MegaBlock 96 Well The MegaBlock 96 Well is ideal for automated processing of sample volumes up to 2.2 ml or handling large quantities of backup samples. Wells with alphanumeric coordination system Raised wells on 0.5 ml ml options Each well 100% leak-proof tested Free from human DNA, DNase/RNase, endotoxins Ideal for long-term storage of samples Also suited for heat sealing Sealing foils/mats available MegaBlock 0.5 / 1.2 / 2.2 ml, PP Solvent-resistant, even to DMSO Autoclavable* For backup storage of pharmaceutical substances For DNA isolation, enzyme and tissue culture applications 2.2 ml square cavities with round bottom wells MegaBlock MegaBlock 1.2 ml, PS transparent Transparent for viewing Ideal for long-term storage of blood samples Pierceable mat cover Order No. Description Volume/ Material round, raised wells 0.5 ml PP 56 pcs./case round, raised wells 1.2 ml PS transparent 32 pcs./case round, raised wells 1.2 ml PP 32 pcs./case square wells 2.2 ml PP 32 pcs./case Adhesive sealing tapes for qpcr Hermetical sealing of micro test plates made from polypropylene, polystyrene and polycarbonate to exclude evaporation and protect samples during applications, storage and transport requires tailor-made tape materials. The Sarstedt range includes a variety of sealing tapes specially designed to meet the sophisticated requirements of PCR, storage of active substances, and High Throughput Screening. All tapes are produced under clean room conditions to exclude contamination with DNase/ RNase and nucleic acids. Highly transparent adhesive tape for quantitative PCR The 50 μm polyolefin film is coated with a smear-free, crystal clear adhesive that shows minimal adhesion at room temperature and is, therefore, exceptionally easy to handle. Thanks to the initially low stickiness, the film on the PCR plate can be easily shifted to the correct position, if necessary. The strong adhesive effect is brought about during PCR and as a result of the heat produced by the cover, minimizing loss by evaporation. Highly transparent film ideally suited for real-time PCR and other fluorescence-based applications Excellent sealing properties with minimized evaporation values due to innovative adhesive No distortion under thermal strain / prevents cross contamination Does not stick to skin or gloves during application Compatible with aqueous solutions and organic solvents like DMSO, acetonitrile and methanol Functional sealing range: -40 C to 110 C Lids and tapes for MegaBlock Order No. Description Cover for 0.5 ml and 1.2 ml MegaBlock, pierceable, 250 pcs./case; suitable for , and pcs./inner case; 10 pcs./bag Cover for 2.2 ml MegaBlock, suitable for pcs./case; 50 pcs./inner case; 10 pcs./bag Silicone mat with pattern adhesive, pre-scored, 240 pcs./case; suitable for 0.5 ml and 1.2 ml MegaBlock 20 pcs./inner case; 10 pcs./bag Aluminium foil, pierceable 100 foils/inner case Acetate film, transparent 100 foils/inner case Adhesive tape, 96-fold perforation, pierceable 100 foils/inner case Strongly adhesive tape, transparent 100 foils/inner case Ordering Information Order No. Description Highly transparent, suited for qpcr with pressure sensitive adhesive 100 tapes/inner case Refer to pp for a detailed description of , , , * Important notes on autoclaving on p

18 Adhesive sealing tapes for qpcr/pcr, automation and sample storage Transparent adhesive tape for end point PCR and sample storage, strongly adhesive The tape is made from a 50 μm polyester film coated with a special adhesive for immediate adhesive effect. Transparent tape with pattern adhesive for multiple applications The PTFE tape for 96-well plates has red-tinted adhesive applied in a pattern to provide adhesive-free zones above each well. Tapes are optically clear with virtually no autofluorescence and are suitable for a broad range of applications, chemicals, and temperatures. Efficient anti-evaporation protection due to strongly adhesive effect Easily removable due to firm end tabs Compatible with aqueous solutions and organic solvents Functional sealing temperature range: -20 to 110 C Ordering Information Order No. Description Packaing Optically clear with virtually no autofluorescence Easily punctured with as little as 0.5 lb force without coring or tearing Adhesive-free zones minimize potential contamination Excellent chemical compatibility One tape fits all concept use for qpcr, PCR, cold storage, HPLC, autosampling, HTS, and proteomics Functional sealing temperature range -80 C to 120 C Transparent adhesive tape for end point PCR and qpcr transparent 100 tapes/inner case The optically transparent tape is made from a 50 μm polyolefin film with perforated end tabs for easy removal. Optically transparent film for standard and real-time PCR Removable without leaving any residue and ideally suited for sample storage to -70 C Compatible with aqueous solutions and organic solvents like DMSO, acetonitrile and methanol Functional sealing temperature range for PCR: up to 105 C Ordering Information Order No. Description Silicone sealing mat with pattern adhesive for storage and frequent sampling The flat, dimple-free 30 mil thick medical grade silicone mat for 96-well plates has redtinted adhesive applied in a pattern to provide adhesive-free zones above each well. Silicone mats are pre-scored for easy piercing without coring, tearing, or obstruction of autosampler probes. Slits reliably reseal after multiple injections, eliminating the need for multiple tapes per plate. Easy to securely apply Pre-scored targets perfect for autosampling, HPLC, and frequent sampling Superior resealability after multiple injections Adhesive-free zones minimize potential contamination Excellent chemical compatability; DMSO resistant Functional sealing temperature range -80 C to 120 C Recommended tape options transparent 100 tapes/inner case Order No. Product description Application Special features Pierceable Material Functional temperature range fold perforated cover tape Processing/DNAsequencer, HPLC, autosampling Easy to pierce, 96 perforated opening w/o adhesive yes Polyester -20 C to 110 C Aluminium foil for PCR and sample storage The heat-resistant, robust, pierceable 38 μm aluminium foil stands out for efficient antievaporation protection and resistance to solvents. Perforated end tabs can be easily removed after attaching the foil Transparent PCR tape, strongly adhesive Transparent PCR and qpcr tape PCR, sample storage PCR, real-time PCR Strongly adhesive, eliminates edges, fringing Good optical transparency, high chemical resistance no Polyester -20 C to 110 C no Polyolefin -70 C to 105 C Tape can be pierced with probes and pipette tips Removable without leaving any residue Ideally suited for storing sample material/compounds to -70 C Compatible with aqueous solutions and organic solvents like DMSO, acetonitrile and methanol Functional sealing temperature range for PCR: up to 105 C Ordering Information Order No. Description aluminium 100 tapes/inner case Adhesive aluminium foil Adhesive, optically highly transparent qpcr tape Transparent tape with pattern adhesive Silicone mat with pattern adhesive prescored Sample storage real-time PCR, fluorescence analyzes qpcr, PCR, cold storage, HPLC, autosampling, HTS Storage, HPLC, autosampling Pierceable light protection for samples, high chemical resistance Highly transparent, pressure-sensitive adhesive, minimal evaporation rates Adhesive-free zones, optically clear, easy to pierce Adhesive-free zones, superior resealability after multiple injections yes Aluminium -70 C to 105 C no Polyolefin -40 C to 110 C yes PTFE -80 C to 120 C yes Silicone -80 C to 120 C 34 35

19 SafeSeal micro tubes with push cap Minimizing the risk of contamination To reduce storage time in open bags and inherent contamination risks, Biosphere plus SafeSeal Micro Tubes are packaged 50 pieces per bag. For most stringent demands in sample preparation, individually wrapped SafeSeal tubes in Biosphere plus quality are available. Optionally available non-sterile 0.5 / 1.5 and 2.0 ml SafeSeal Micro Tubes in PCR Performance Tested quality: DNA-, DNase-, RNase- and PCR inhibitor-free. Fig.: SafeSeal Micro Tube individually wrapped 0.5 / 1.5 and 2.0 ml Micro Tubes with sterile Biosphere plus purity are routinely used when DNA-, DNase-, RNase-, PCR inhibitor-, ATP-free and non-pyrogenic conditions are essential. Superior to standard reaction tubes, Biosphere plus Micro Tubes have been developed and continually enhanced to suit the requirements in medical and molecular-biological research. Secure SafeSeal cap locking Ordering Information Micro Tubes with SafeSeal push cap The 0.5 / 1.5 and 2.0 ml SafeSeal Micro Tube features a closing mechanism which prevents unintentional cap opening under thermal stress. Wide hinge for easy and precise closure Large writing space on lid Centrifugation up to 30,000 x g (2.0 ml Micro Tubes up to 25,000 x g) Volume 0.5 ml 1.5 ml 2.0 ml Description Order No. Order No. Order No /bag 500/inner case 2,000/case /bag 1,000/inner case 2,000/case /bag 1,000/inner case 2,000/case /bag 250/inner case 500/case /bag 250/inner case 500/case /bag 250/inner case 500/case individually wrapped 50/inner case 100/case individually wrapped 60/inner case 120/case individually wrapped 60/inner case 120/case 36 37

20 UV-transparent disposable cuvettes for photometric analyzes in the UV range 220 nm The plastic material of the half micro cuvette enables measurements in the UV range from wave lengths of 220 nm. Contamination caused by the repeated rinsing processes required for quartz glass cuvettes can be avoided by using the UV-transparent disposable cuvette. Apart from non-certified UV cuvettes, our range includes a certified option individually sealed with a cap and packed in a Styrofoam container to protect from scratches. Typical applications and product features Photometric analysis of nucleic acids at 260 nm and proteins at 280 nm Low UV absorbance for DNA, RNA and protein quantification Compatible with photometers of 8.5 mm and 15 mm light center height: Eppendorf Biophotometer, ThermoSpectronic, Perkin Elmer, Bio-Rad, Analytik Jena etc. Minimizes absorbance deviations due to consistently accurate optical quality Economical low-cost alternative to quartz cuvettes Certified UV cuvettes (DNA-/RNase-/Protein-free) individually sealed with caps optionally available for safe sample storage and prevention of contamination Technical specification Min. sample volume: Optical path length: Basic absorbance: External dimensions (W x D x H): Light center height (LCH): 50 μl 10 mm A at 260 nm A at 280 nm 12.5 x 12.5 x 45 mm 8.5 mm and 15 mm For measurements in the short-wave UV range below 300 nm, the material-related self-absorptive characteristics of PS and acrylic (PMMA) cuvettes increase distinctively as their transmission ration decreases. Ordering Information UV Cuvettes Order No. Length LCH Wavelength in nm UV cuvette Transmission in % Acrylic cuvette Polystyrene cuvette mm 100 pcs. in Styrofoam box; 400 pcs./case mm 100 pcs. ind. sealed with lid, in Styrofoam box; 400 pcs./case certified DNA-/RNase-/Protein-free mm 100 pcs. in Styrofoam box; 400 pcs./case mm 100 pcs. ind. sealed with lid, in Styrofoam box; 400 pcs./case certified DNA-/RNase-/Protein-free nm Transmission values in individual cuvettes UV cuvette Acrylic cuvette Polystyrene cuvette 38 39

21 Please call if you require more information This document may contain information on products that may not be available in particular countries Technical modifications reserved SARSTEDT Inc St. James Church Road P.O. Box 468 Newton, NC Tel. (800) Fax (828)