Cell Lab. Problem: How do bacterial, animal and plant cells differ?
|
|
- Jeffrey Craig
- 6 years ago
- Views:
Transcription
1 Cell Lab Problem: How do bacterial, animal and plant cells differ? Objective: Create a list of characteristics and criteria to identify bacteria, animal and plant cells. Materials: Light microscope Microviewer image of Bacteria Elodea leaf Iodine Unknown B Glass Slide Methylene blue stain Water Frog/Fish Blood Coverslip Toothpick Onion skin Unknown A Procedure: Pre-Lab: 1. Complete the pre-lab questions #1-4. Part 1: Bacterial Cells 1. Obtain a microviewer and microviewer image of a prepared bacterial slide. 2. A generalized bacterial cell has been drawn for you. 3. Label all of the visible parts of the cell. 4. Fill in the appropriate column in Data Table Return the microviewer and microviewer image of a prepared bacterial slide. Part 2: Human Cheek Cells 1. Obtain supplies 1 microscope, 1 slide, 1 coverslip, 1 toothpick 2. Using the flat end of the toothpick, gently scrape the inside of your cheek. (Gently we want to look at cheek cells, not blood cells). 3. Put the cheek cells from the toothpick onto the center of a slide. 4. Go to the Methylene blue station and add one drop of Methylene blue directly on the cheek cells. 5. Add the coverslip. 6. View the cheek cell under low power. Be sure the sample is centered in the field of view. 7. View the cheek cell under medium power. Be sure the sample is centered in the field of view. 8. View the cheek cell under high power. Be sure the sample is centered in the field of view. 9. On your data sheet draw a single cheek cell as you see it under high power. Be sure to include the magnification in the space provided. 10. Label all of the visible parts of the cell. 11. Fill in the appropriate column in Data Table Clean and dry slide and coverslip (remember to save trees use the smallest piece of paper towels possible). 13. Throw away the toothpick (we won t reuse these). Part 3: Elodea Cells 1. Obtain a leaf of the Elodea plant. 2. Put the leaf on the center of the slide try to get it to be as flat as possible. 3. Go to the DI water station and add 1 drop of water to the slide. 4. Add the coverslip. 5. View the Elodea leaf under low power. Be sure the sample is centered in the field of view. 6. View the Elodea leaf under medium power. Be sure the sample is centered in the field of view. 7. View the Elodea leaf under high power. Be sure the sample is centered in the field of view. 8. On your data sheet draw a single Elodea leaf cell as you see it under high power. Be sure to include the magnification in the space provided. 9. Label all of the visible parts of the cell. 10. Fill in the appropriate column in Data Table Clean and dry slide and coverslip (remember to save trees use the smallest piece of paper towels possible). 12. Throw away the Elodea leaf.
2 Part 4: Onion Skin Cells 1. Obtain supplies 1 microscope, 1 slide, 1 coverslip, 1 piece of onion 3. Prepare a slide of the onion skin by: a. Peal a thin layer from the inside of a piece of onion. b. Lay the thin layer on the slide. Try to arrange the sample in a single, unwrinkled layer. c. Go to the Iodine station and add one drop of iodine on the onion skin sample. d. Add a cover slip. e. Discard the rest of the sample piece. 4. View the onion under low power. Be sure the sample is centered in the field of view. 6. View the onion under medium power. Be sure the sample is centered in the field of view. 7. View the onion under high power. Be sure the sample is centered in the field of view. 8. On your data sheet draw a single cell of the onion skin sample as you see it under high power. Be sure to include the magnification in the space provided. 9. Label all of the visible parts of the cell. 10. Fill in the appropriate column in Data Table Clean and dry slide and coverslip (remember to save trees use the smallest piece of paper towels possible). 12. Throw away the onion skin. 13. Return the slide and coverslip. Part 5: Frog/Fish Blood 1. Obtain a prepared slide of the blood sample. 2. View the blood under low power. Be sure the sample is centered in the field of view. 6. View the blood under medium power. Be sure the sample is centered in the field of view. 7. View the blood under high power. Be sure the sample is centered in the field of view. 8. On your data sheet draw a single blood cell from the sample as you see it under high power. Be sure to include the magnification in the space provided. 9. Label all of the visible parts of the cell. 10. Fill in the appropriate column in Data Table Return the prepared slide. Application: Part 6: Unknown A 1. Obtain a prepared slide of Unknown A. 2. View Unknown A under low power. Be sure the sample is centered in the field of view. 3. View Unknown A under medium power. Be sure the sample is centered in the field of view. 4. View Unknown A under high power. Be sure the sample is centered in the field of view. 5. On your data sheet draw a single cell of the Unknown A sample as you see it under high power. Be sure to include the magnification in the space provided. 6. Label all of the visible parts of the cell. 7. Fill in the appropriate column in Data Table Return the prepared slide. Part 7: Unknown B 1. Obtain a prepared slide of Unknown B. 2. View Unknown B under low power. Be sure the sample is centered in the field of view. 3. View Unknown B under medium power. Be sure the sample is centered in the field of view. 4. View Unknown B under high power. Be sure the sample is centered in the field of view. 5. On your data sheet draw a single cell of the Unknown B sample as you see it under high power. Be sure to include the magnification in the space provided. 6. Label all of the visible parts of the cell. 7. Fill in the appropriate column in Data Table Return the prepared slide.
3 Cell Lab Pre-Lab Questions 1. Summarize the steps to create a wet mount slide. Microbiologist: Period Date 2. What objective lens should you start with when you are trying to find your sample? 3. What is the total magnification for the high power objective lens? (Show your work) 4. What was your estimated diameter of the field of view for each power objective lens? Objective Lens Total Magnification Field of View Diameter (mm) Field of View Diameter (µm) Low Medium High Data: 1. Label the cell structures of the bacterium. Bacteria Sample This is a cartoon and not a real image! Magnification: approximately 20000x 2. How do you know the cell in the image above is a bacteria? Use the structures and characteristics to justify your answer.
4 Data Cheek Cells Elodea Cells Onion Skin Cells Blood Cells
5 Application Unknown A Unknown B Additional Box (if needed) Additional Box (if needed)
6 Cell Lab Microbiologist: Period Date Data Table 1 1. Complete the following table based on what you could see in the Cell Lab. (Mark an X if the structure was present and leave it blank if the structure was absent.). 2. Determine if the cell is a Prokaryote or Eukaryote. 3. Estimate the size of a single cell. Cell Part Bacteria Cell Cheek Cell Elodea Cell Onion Cell Blood Cell Unknown A Unknown B Cytoplasm Nucleus Chloroplast Cell wall Cell membrane Estimated size in micrometers 3 µm Prokaryote or Eukaryote Post Lab Questions 1. What structures did you see in all seven samples? 2. a. Which cells were eukaryotes? b. What structures did you see in all the eukaryotic cells? 3. a. Which samples were plant cells? (Use evidence from Data Table 1 to explain your answer!) b. Which samples were animal cells? (Use evidence from Data Table 1 to explain your answer!)
7 4. Classify each unknown as a plant cell, animal cell, or bacteria cell. Use the characteristics from your drawings/observations and criteria from Data Table 1 as evidence in your explanation. (Identifications without explanations are worth zero points!) Sample Classification Explanation Unknown A Unknown B 5. Is the nucleus always found in the center of the cell? 6. Why did we use stains (methylene blue and iodine) when observing the cells under the microscope? 7. Which organelle was moving in the Elodea cells? WHY? 8. Which plant organelle are the onion cells missing? WHY? This lab sign out must be signed before the end of the lab class. Lab Sign Out task initials task initials task initials Partners Lab station is clean and trash is disposed of All equipment cleaned and accounted for Lab equipment returned to correct location
Cells. He who can no longer pause to wonder and stand rapt in awe is as good as dead; his eyes are closed. -Albert Einstein. Studying Cell Variety
Cells He who can no longer pause to wonder and stand rapt in awe is as good as dead; his eyes are closed. -Albert Einstein Objectives: Studying Cell Variety When we have our quiz, you need to be able to
More information-------------------------------------- Biology Name Summary Sbeet Cell Structure Lab Class Date --------------------~ ---------- A. Cork Cells: Wet mount I Higb Power 1. Is it easier to see individual
More informationActivity 2.1. Activity 2.2. Looking at animal cells. Looking at plant cells
Activity 2.1 Looking at animal cells Skills C1, C2 a source of animal cells, for example some macerated liver or scrapings from the lining of the trachea from a set of sheep or other lungs (obtainable
More informationActivity 5.1.4: Gram Staining
Activity 5.1.4: Gram Staining Introduction In the last activity, you isolated the bacteria that was responsible for Anna s illness and performed a gross examination of the resultant colonies. While gross
More informationCells Reproduction and Inheritance
S2 Biology 3 Cells Reproduction and Inheritance Cells Cells are the tiny building blocks that make up all living things. Living things can be unicellular or multicellular. Unicellular = organism made of
More informationCell Biology Homework
Cell Biology Homework NAME: CLASS: 1 1. The diagram below shows two cells. Cell Structure a) Complete the table below to give the name and functions of the parts labelled A, B and C. (2) Letter Part Function
More informationScience Class 7 Topic: From Cells to Organisms Reinforcement Worksheet
Science Class 7 Topic: From Cells to Organisms Reinforcement Worksheet Name: Sec: Date: Q.1 Choose the best answer. 1. The cell occurs in pairs: a) Guard cell b) Palisade cell c) Root hair cell d) Sperm
More informationCell Fractionation Supplement
How to use this supplement: Exercise 8 Cell Fractionation Supplement Read the first four pages of the exercise in your manual (Introduction & Principles). You do not need to complete the prelab exercise
More informationCurriculum Catalog
2017-2018 Curriculum Catalog Biology 2017 Glynlyon, Inc. Table of Contents UNIT 1: TAXONOMY: KEY TO ORGANIZATION... 1 UNIT 2: CHEMISTRY OF LIFE... 2 UNIT 3: CELLS... 3 UNIT 4: CELL DIVISION AND REPRODUCTION...
More informationMethods of Culturing Microorganisms. Chapter 3. Five Basic Techniques of Culturing Bacteria. Topics
Chapter 3 Topics Methods of Culturing Microorganisms Microscope (History, Types, Definitions) Staining (Gram s) Methods of Culturing Microorganisms Five basic techniques of culturing Media Microbial growth
More informationDeciding how to handle waste produced from manufacturing
27 Reclaiming the Metal l a b o r at o ry Deciding how to handle waste produced from manufacturing is challenging. It can be diluted or incinerated, as you saw in previous activities. In some cases, it
More informationComplete the table by giving the letter labelling the organelle that matches the function. Function of organelle
Q1.The diagram shows a eukaryotic cell. (a) Complete the table by giving the letter labelling the organelle that matches the function. Function of organelle Letter Protein synthesis Modifies protein (for
More informationWarning. Warning. Caution
Case label user s manual 書 Thank you for purchasing OLYMPUS Micro cantilever. Please read this manual carefully before use. s in a plastic case are included
More informationPLP 6404 Epidemiology of Plant Diseases Spring 2015 LAB 2 PHASES IN THE DISEASE CYCLE: GREENHOUSE AND LAB EXERCISE
PLP 6404 Epidemiology of Plant Diseases Spring 2015 LAB 2 PHASES IN THE DISEASE CYCLE: GREENHOUSE AND LAB EXERCISE "Variation in Host-Pathogen Interactions and its Effect on Epidemic Development" Purpose:
More informationBiology Test Review Microorganisms
Name: Period: Biology Test Review Microorganisms Use your booklet, notes, & quizzes to complete this review. 1. Define the following terms using a few key words: a. Host cell - victim of the virus b. Retrovirus
More informationChapter 03 - Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of microorganisms
Microbiology: A Systems Approach 4th Edition Cowan Test Bank Completed download: https://testbankreal.com/download/microbiology-systems-approach-4thedition-test-bank-cowan/ (Downloadable package TEST BANK
More informationThrowing the Dice: Teaching the Hemocytometer
Tested Studies for Laboratory Teaching Proceedings of the Association for Biology Laboratory Education Vol. 32, 397-401, 2011 Throwing the Dice: Teaching the Hemocytometer Sarah Salm 1 and Jessica Goldstein
More informationLab: Blood Smear and RBC Count
2014-09- 01 Blood Page 1 of 5 Lab: Blood Smear and RBC Count Aim: Learn to count cells, observe and identify different blood cells in a smear, quantify their proportions and count RBCs per µl (mm^3) using
More informationEXTRA CREDIT - EXPERIMENT G ELECTROCHEMISTRY ACTIVITY OF METALS
EXTRA CREDIT - EXPERIMENT G ELECTROCHEMISTRY ACTIVITY OF METALS INTRODUCTION The objective of this experiment is to develop an abbreviated activity series of metals using: 1. Displacement reactions 2.
More informationMeasuring Total Solids (Total Proteins) Using a Refractometer
Year Group: BVSc3 + Document number: CSL_L01 Equipment list: Measuring Total Solids Equipment for this station: Blood sample in microhaematocrit (capillary) tube Refractometer Gloves Paper towel or tissue
More informationBacteria Transformation
Background Information: PART I: Bacteria are the most common organisms modified by genetic engineers due to the simple structures of bacteria cells compared to those of eukaryotic cells. Engineers are
More informationPreparation of tissues for study
Preparation of tissues for study HISTOLOGY : It is the branch of science which deals with the microscopic study of normal tissue HISTOPATHOLOGY : It is the branch of science which deals with the microscopic
More informationLesson 1.2. Suspect Profiling. Estimated time: One 50 min period. Instructional overview. Instructional objectives. Assessment
Lesson 1.2 Suspect Profiling Estimated time: One 50 min period Instructional overview Lesson 1.2 will introduce students to criminal profiling techniques. Students will use these techniques to investigate
More informationBio EOC Topics for Living Things, Metric Measurement, Microscope and The Scientific Method
Bio EOC Topics for Living Things, Metric Measurement, Microscope and The Scientific Method Laboratory Equipment o Identification of laboratory equipment; for example beaker, triple beam balance, microscope,
More informationReinforcement. Cells and Life CHAPTER 1 LESSON 1
Reinforcement Cells and Life LESSON 1 Directions: In numbers 1 through 4 below, a code letter has been substituted for each letter of the alphabet. To find out what the sentence says, use the following
More informationDNA RESTRICTION ANALYSIS
DNA RESTRICTION ANALYSIS In this experiment, DNA from the bacteriophage Lambda (48,502 base pairs in length) is cut with a variety of restriction enzymes and the resulting fragments are separated using
More informationBacterial Counts - Quantitative Analysis of Microbes
Bacterial Counts - Quantitative Analysis of Microbes Introduction: It is often important to know not only what types of bacteria are in a sample but also how many of them are present. Food manufacturers
More informationPURE CULTURE TECHNIQUES
PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,
More informationHuman Cheek Cell Labeled Diagram
Human Cheek Cell Labeled Diagram Free PDF ebook Download: Human Cheek Cell Labeled Diagram Download or Read Online ebook human cheek cell labeled diagram in PDF Format From The Best User Guide Database
More informationElectron Microscopy (EM) Grid
Anirban Som 25-01-14 Instrumental technique presentation Electron Microscopy (EM) Grid What I will talk about Some basic topics about EM grid Home-made grid preparation Grid cleaning Carbon coating and
More informationProject 7: Wound Cultures and Identification
Project 7: Wound Cultures and Identification Readings: https://labtestsonline.org/understanding/analytes/wound-culture/tab/test Identification of Gram-Positive & Gram-Negative Bacteria Guide to laboratory
More informationRead section 2.2 and take notes then answer the questions: Define:
Due Wednesday, September 6 Read section 2.1 and take notes as you read then answer the questions: Define: Stimulus - Response - Growth - Cell - Energy - Answer: 1. List an example of a response. 2. What
More informationPrimerdesign TM Ltd. Eubacteria. genesig Easy Kit for use on the genesig q reactions. For general laboratory and research use only
Primerdesign TM Ltd Eubacteria genesig Easy Kit for use on the genesig q16 50 reactions For general laboratory and research use only 1 genesig Easy: at a glance guide For each DNA test Component Volume
More informationCreate a model to simulate the process by which a protein is produced, and how a mutation can impact a protein s function.
HASPI Medical Biology Lab 0 Purpose Create a model to simulate the process by which a protein is produced, and how a mutation can impact a protein s function. Background http://mssdbio.weebly.com/uploads/1//7/6/17618/970_orig.jpg
More informationPREPARATION OF HISTOLOGICAL SPECIMENS
PREPARATION OF HISTOLOGICAL SPECIMENS Histo-techniques Preparation of tissue for microscopic examination Series of processes Ultimate aim to make tissue visible as it is Pathology Vs Anatomy Steps vary
More informationPage 1. Name: 1) Which letter indicates a cell structure that directly controls the movement of molecules into and out of the cell?
Name: 1) Which letter indicates a cell structure that directly controls the movement of molecules into and out of the cell? A) A B) B C) C D) D 2) A single-celled organism is represented in the diagram
More informationDETERMINATION of the EMPIRICAL FORMULA
DETERMINATION of the EMPIRICAL FORMULA One of the fundamental statements of the atomic theory is that elements combine in simple whole number ratios. This observation gives support to the theory of atoms,
More informationHow to perform-control immunostaining experiment - microscopist subjective point of view. Pawel Pasierbek
How to perform-control immunostaining experiment - microscopist subjective point of view. Pawel Pasierbek Immunolabeling and fluorescent detection became such a standard procedure in the biomedical research
More informationCell Culture Flasks DATA SHEET
DATA SHEET Cell Culture Flasks In research cell culture flasks are used as a matter of routine for the cultivation of eukaryotic cells. They are optimal products for adherent cells as well as for suspension
More informationEQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY
EQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY a) Autoclave: An autoclave is a device used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 C for around
More informationAnswers to the multiple choice questions are at the bottom of the last page of this document.
Review for Unit Test #2: Cell Parts, Functions and Protein Synthesis, Answers Answers to the multiple choice questions are at the bottom of the last page of this document. 1. Know all of the material on
More informationLambda (λ) DNA Restriction Digest and Electrophoresis Lab
Lambda (λ) DNA Restriction Digest and Electrophoresis Lab Procedure DAY ONE: restriction digestion Today we will be exposing the lambda DNA to restriction enzymes. For background knowledge, make sure you
More informationMicrobe Mission. Casa Grande Science Olympiad Invitational 2011
Microbe Mission Casa Grande Science Olympiad Invitational 2011 Station 1: Parts of a Microscope 1. Label the parts of the microscope. (A-K) 2. What is the greatest magnification, or power, available on
More information3. The arrows in the diagram below indicate the movement materials into and out of a single-celled organism.
Part A Questions 1. In 1910, Thomas Morgan discovered a certain pattern of inheritance in fruit flies known as sex linkage. This discovery extended the ideas of inheritance that Gregor Mendel had discovered
More informationManatee County Sheriff s Office PROPERTY AND EVIDENCE
Manatee County Sheriff s Office PROPERTY AND EVIDENCE 1 What Every LEO Should Know About DNA Evidence 2 Similar to fingerprints DNA is similar to fingerprint analysis in how matches are determined. When
More information2014 Science Supply List Science 500
2014 Science Supply List Science 500 2014 Glynlyon, Inc. Released April 1, 2014 Table of Contents UNIT 1: CELLS... 1 UNIT 2: PLANTS: LIFE CYCLES... 2 UNIT 3: ANIMALS: LIFE CYCLES... 3 UNIT 4: BALANCE IN
More informationIn order to do transformation, the gene to be transferred is placed into a plasmid. This is done with the help of restriction enzymes, 7
Fluorescent Protein Transformation Student Background Genetic transformation occurs when a cell takes up (i.e. takes inside) and expresses a new piece of genetic material DNA. Genetic transformation literally
More informationBIOLOGY 163 LABORATORY. THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014)
BIOLOGY 163 LABORATORY THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014) Bacteria are single-celled prokaryotic organisms. As bacterial cells take in nutrients from their
More informationBacterial Cytology 1 BACTERIAL CYTOLOGY
Bacterial Cytology 1 BACTERIAL CYTOLOGY In the previous two exercises you learned techniques for aseptic manipulation and microscopic observation of microorganisms. In this week's exercise you will employ
More informationScience Supply List Science 500
2017-2018 Science Supply List Science 500 2017 Glynlyon, Inc. Table of Contents UNIT 1: CELLS... 1 UNIT 2: PLANTS: LIFE CYCLES... 2 UNIT 3: ANIMALS: LIFE CYCLES... 3 UNIT 4: BALANCE IN NATURE... 4 UNIT
More informationChapter 10: Classification of Microorganisms
Chapter 10: Classification of Microorganisms 1. The Taxonomic Hierarchy 2. Methods of Identification 1. The Taxonomic Hierarchy Phylogenetic Tree of the 3 Domains Taxonomic Hierarchy 8 successive taxa
More informationPurification of mfp. from an Overnight Culture. Laboratory 17
Purification of mfp from an Overnight Culture When scientists at a therapeutics company, like Amgen, have successfully identified a promising therapeutic protein, two objectives would be to locate and
More informationLab 1A: Microscopy I. Name: Section:
Lab 1A: Microscopy I A response is required for each item marked: (# ). Your grade for the lab 1 report (1A and 1B combined) will be the fraction of correct responses on a 50 point scale[(# correct/# total)
More informationSection A: Prokaryotes Types and Structure 1. What is microbiology?
Section A: Prokaryotes Types and Structure 1. What is microbiology? 2. Compare and contrast characteristics of each bacterial type: Eubacteria and Archaebacteria. Eubacteria Both Archaebacteria 3. Label
More information3.1.4 DNA Microarray Technology
3.1.4 DNA Microarray Technology Scientists have discovered that one of the differences between healthy and cancer is which genes are turned on in each. Scientists can compare the gene expression patterns
More informationtest 7 3. What is the main function of a vacuole in a cell?
test 7 Name: Date: 1. ase your answer(s) to the following question(s) on the diagram below and on your knowledge of biology. The diagram represents a model cell setup. The locations of three different
More informationWorms and their environment
s and their environment Objectives: The student will observe the growth of in different environments. The student will become familiar with biotechnology techniques. Specifically, how to manipulate organisms
More information4_ANALYSIS OF PHYTOPLANKTON SAMPLES
4_ANALYSIS OF PHYTOPLANKTON SAMPLES The identification and counting of phytoplankton cells is something that takes much patience, practice and experience to do correctly. There are a number of taxonomic
More informationSample Preparation 3D Cell Explorer. Version 1.1 July 2015 Sabine Bautz
Sample Preparation 3D Cell Explorer Version 1.1 July 2015 Sabine Bautz Contents 1. Important points to keep in mind for the sample preparation 3 2. Sample preparation specifications and data sheet 4 3.
More informationIntroductory Microbiology Laboratory Manual
Introductory Microbiology Laboratory Manual MB 230 Winter 2018 Lab Rooms: Nash 304/316 Labs start week of 1/08/18 You MUST attend lab during the first week of the term or you will automatically be dropped
More informationA Level. A Level Biology. Cells, Microscopes, Cell Cycle and Immunity Questions. AQA, OCR, Edexcel. Name: Total Marks: Page 1
AQA, OCR, Edexcel A Level A Level Biology Cells, Microscopes, Cell Cycle and Immunity Questions Name: Total Marks: Page 1 Q1.The diagram shows a eukaryotic cell. (a) Complete the table by giving the letter
More informationBrdU IHC Kit. For the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells
K-ASSAY BrdU IHC Kit For the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells Cat. No. KT-077 For Research Use Only. Not for Use in
More informationIntroduction to Bioinformatics
Introduction to Bioinformatics Contents Cell biology Organisms and cells Building blocks of cells How genes encode proteins? Bioinformatics What is bioinformatics? Practical applications Tools and databases
More informationCell Growth and Reproduction
Cell Growth and Reproduction Robert Hooke was the first person to describe cells, in the year 1665. He was looking through his microscope at a piece of cork when he noticed a lot of repeating honeycomb
More informationCONDUCTING A WASTE AUDIT AT CONCORDIA
CONDUCTING A WASTE AUDIT AT CONCORDIA Drafted by: Faisal Shennib, Environmental Coordinator Environmental Health and Safety Last Edited: 2015-02-02 OVERVIEW Waste audits are a critical tool for assessing
More informationFrequency of Keyword Totals - (All LE Regents Exams)
Frequency of Keyword Totals - (All LE Regents Exams) KEYWORD COUNT KEYWORD COUNT ecosystem 58 DNA 48 energy pyramid 19 graph 19 scientific method 19 photosynthesis 43 decomposer 18 human impact 42 clone
More informationAuthor. Intended Audience
Microbial Discovery Activity Build a Bacterium Scavenger Hunt Author Janelle Hare, PhD Biological & Environmental Sciences Morehead State University, Morehead, KY jm.hare@morehead-st.edu Intended Audience
More informationNote that Methylene Blue-stained cultures may require an additional washing step if the second wash is still very blue in appearance.
Introduction: Cell culture in Alvetex Scaffold allows the formation of multilayered, high-density cell populations which approximate the complexity and structure of in vivo tissues. When viewing an unstained,
More informationEffects of Turbidity on Living Things
3.4 Investigate Effects of Turbidity on Living Things Macroinvertebrates can serve as an indicator of water quality in an ecosystem. That is just one example of how living things can serve as an indicator
More informationAseptic Techniques. A. Objectives. B. Before coming to lab
Aseptic Techniques A. Objectives Become familiar with 1. The ubiquity of microorganisms (see Note 1) 2. Aseptic techniques (see Note 2) 3. Standard methods for growing/observing microorganisms (see Note
More informationHeat and Thermal Energy
Next Generation Science Standards NGSS Science and Engineering Practices: Asking questions and defining problems Developing and using models Planning and carrying out investigations Analyzing and interpreting
More informationDepartment of Microbiology, Lab 016 instructions
Protocol for standard FISH and DOPE-FISH for prokaryotes (slightly modified from Amann, 1995, note, for other modifications or other microorganisms like eukaryotes, consult special literature or check
More informationProcedure for Stool Routine & Microscopy
Procedure for Stool Routine & Microscopy : To perform Gross & Physical examination of Stool Variables : Specimen collection, Labelling of specimen, Name, Identifying number, date, time Gross Examination
More informationBi 1x Spring 2014: E. coli Growth Curves
Bi 1x Spring 2014: E. coli Growth Curves 1 Overview In this lab, you will investigate growth of the bacterium E. coli, watching the growth in two ways. First, you will determine growth rate in a solution
More informationNATURE OF MICROBES WORKBOOK
NATURE OF MICROBES WORKBOOK Name: Tutor Group: 1 Microbes and Mankind 4. NATURE OF MICROBES 1. OBJECTIVES: What are microbes and are there different types? How are they seen? How can they be grown? How
More informationTYPES OF CHEMICAL REACTIONS PART I INTRODUCTION
EXPERIMENT 10 (2 Weeks) Chemistry 100 Laboratory TYPES OF CHEMICAL REACTIONS PART I INTRODUCTION It is useful to classify reactions into different types, because products of reactions can be predicted.
More informationElectron Microscopy Sciences
Electron Microscopy Sciences INSTRUCTIONAL MANUAL CAT. 65212-10 Via-Cell Bioaerosol Sampling Cassette P.O. Box 550 s1560 Industry Road s Hatfield PA 19440 1 Introduction Thank you for your purchase of
More informationMICROBIOLOGY 20 (course #3124)
Stephen Brown (instructor) Los Angeles Mission College, Fall 2008 Lab: TTh 5:30-6:55, INST 2016 email: BrownST@lamission.edu Lecture: TTh 7:00-8:25, INST 2001 voicemail: 818-364-7665 office hours: TTh
More informationTotal Test Questions: 66 Levels: Grades Units of Credit: 1.0 STANDARD 2. Demonstrate appropriate use of personal protective devices.
DESCRIPTION Biotechnology is designed to create an awareness of career possibilities in the field of biotechnology. Students are introduced to diagnostic and therapeutic laboratory procedures that support
More informationEutrophication: Too Much of a Good Thing?
Name Class Date Skills Practice Lab DATASHEET FOR IN-TEXT LAB Eutrophication: Too Much of a Good Thing? Plants depend on nutrients such as phosphates and nitrates to survive. However, when people release
More informationI. Conditions for Phagocytosis: Phagocytosis of Paramecium by Pelomyxa carolinensis Amoeba
Bio 219 Homepage I. Conditions for Phagocytosis: Phagocytosis of Paramecium by Pelomyxa carolinensis Amoeba Michael Smith Wheaton College Norton, MA 02766 In collaboration with Samara Audet Original Post:
More informationCytoPainter Golgi Staining Kit Green Fluorescence
ab139483 CytoPainter Golgi Staining Kit Green Fluorescence Instructions for Use Designed for the detection of Golgi bodies by microscopy This product is for research use only and is not intended for diagnostic
More informationUNIT ONE Performance Objective Critical Attributes Benchmarks/Assessment
Curriculum Standard: The student will demonstrate an understanding of the ways biology affects their lives and the industry of agriculture. The student will use the scientific method and research techniques
More informationName Per AP: CHAPTER 27: PROKARYOTES (Bacteria) p559,
AP: CHAPTER 27: PROKARYOTES (Bacteria) p559, 561-564 1. How does the bacterial chromosome compare to a eukaryotic chromosome? 2. What is a plasmid? 3. How fast can bacteria reproduce? 4. What is a bacterial
More informationSlide 1. Welcome to the on-line training course for Waste Management!
Slide 1 Welcome to the on-line training course for Waste Management! Slide 2 Course Information Course Title: Waste Management Non-Clinical Regulations/Standards: PA Department of Health, PA Department
More informationMelting Point 1. Figure 2. A close-up of the "business end" of the Mel-Temp apparatus. Figure 1. The mel-temp device.
Melting Point 1 The temperature at which a solid melts is known as the melting point (MP) of that substance. The melting point is a physical property of a solid and can be used to help identify a substance.
More informationName: Class: Biology Weekly Packet January th, 2013 Tuesday January 22, 2013
Name: Class: Biology Weekly Packet January 22-25 th, 2013 Tuesday January 22, 2013 Graphs The x- axis is horizontal and is the dependent variable. The y- axis is vertical and is the independent variable.
More informationCONSERVATION OF MATTER AND CHEMICAL PROPERTIES
1 CONSERVATION OF MATTER AND CHEMICAL PROPERTIES I. OBJECTIVES AND BACKGROUND The object of this experiment is to demonstrate the conservation of matter- or more particularly, the conservation of "atoms"
More informationExercise 19. Fungi: Molds and Yeasts F10 Or The Rotten World Around Us
Exercise 19 119 Fungi: Molds and Yeasts F10 Or The Rotten World Around Us INTRODUCTION: Student Learning Objectives: After completing this exercise students will: a. Define the terms Saprophyte, Mycosis,
More informationMaterials and Methods Materials Required for Fixing, Embedding and Sectioning. OCT embedding matrix (Thermo Scientific, LAMB/OCT)
Page 1 Introduction Tissue freezing and sectioning is a rapid method of generating tissue samples (cryosections) for histological analysis, and obviates the need for wax embedding. The method is popular
More informationSergeant System s Immune System Defense Team Webquest
Name Sergeant System s Immune System Defense Team Webquest Hello! I m Sergeant Cell and I am in charge of the Special Operations Teams here at the ImmunoSystem Police Station. 2 Thanks so much for coming
More informationMolecular Scissors: Lambda Digest Student Materials
Molecular Scissors: Lambda Digest Student Materials Introduction 2 Pre-Lab Questions. 5 Lab Protocol 6 Data Collection Worksheet. 9 Post-Lab Questions and Analysis.. 10 Plasmid Maps. 13 Last updated: August
More informationASEPTIC TRANSFER & PURE CULTURE TECHNIQUES
ASEPTIC TRANSFER & PURE CULTURE TECHNIQUES GENERAL GUIDELINES & REMINDERS: SAFETY: NO EATING OR DRINKING IN THE LAB! Wash your hands with soap both BEFORE and AFTER lab, and, in addition, when you have
More informationBIOLOGY/HUMAN BIOLOGY BY1
Candidate Name Centre Number Candidate Number 2 GCE AS/A level 1071/01 New AS BIOLOGY/HUMAN BIOLOGY BY1 P.M. MONDAY, 1 June 2009 1 1 2 hours For Examiner s use Question Maximum Mark Mark Awarded 1 8 2
More informationLAB 6: Agarose Gel Electrophoresis of Restriction Digested Plasmid DNA
LAB 6: Agarose Gel Electrophoresis of Restriction Digested Plasmid DNA I. Objectives The purpose of today s lab is to learn how to set up and run an agarose gel, separate DNA fragments on the gel, and
More informationBaraa Ayed AL-Odat. Israa Ayed. Heba kalbouneh
1 Baraa Ayed AL-Odat Israa Ayed Heba kalbouneh Introduction: "lecture #1" The name " histology " is derived from the Greek words: "histo" means a tissue and "logos" means the study of. So, Histology mean
More informationCambridge International Examinations Cambridge International Advanced Subsidiary and Advanced Level
ambridge International Examinations ambridge International Advanced Subsidiary and Advanced Level *8744875516* BIOLOGY 9700/22 Paper 2 AS Level Structured Questions October/November 2016 1 hour 15 minutes
More informationExperiment 3: The Chromatography of Organic Compounds
Experiment 3: The Chromatography of Organic Compounds INTRODUCTION Very often, in an organic synthesis, a reaction will proceed to produce multiple products or perhaps will only partially form the desired
More informationAnalysis of Calcium Carbonate Tablets
Experiment 9 Analysis of Calcium Carbonate Tablets Prepared by Ross S. Nord, Eastern Michigan University PURPOSE To perform a gravimetric exercise to determine weight percent of active ingredient in a
More information