Product Datasheet. Acetylcholinesterase/ACHE Antibody (HR2) NB Unit Size: 200uL. Store at -20C. Avoid freeze-thaw cycles.

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1 Product Datasheet Acetylcholinesterase/ACHE Antibody (HR2) NB Unit Size: 200uL Store at -20C. Avoid freeze-thaw cycles. Publications: 1 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 3/19/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Acetylcholinesterase/ACHE Antibody (HR2) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer 200uL 1 mg/ml Store at -20C. Avoid freeze-thaw cycles. Monoclonal HR2 0.05% Sodium Azide IgG2b Protein A purified PBS Page 1 of 5 v.20.1 Updated 3/19/2018 Product Description Host Gene ID 43 Gene Symbol Species Reactivity Notes Marker Specificity/Sensitivity Immunogen Product Application Details Applications Mouse ACHE Human, Bovine, Feline, Rabbit, Mouse (Negative), Rat (Negative) No cross-reactivity with Frog. Early Neuronal Development Marker This antibody does not detect butyrylcholinesterase (BChE). Purified human cerebellar acetylcholinesterase. ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation Recommended Dilutions ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:50 Application Notes Images IHC: Staining of AChE in human brain samples results in staining of nerve fibers and terminals. Cannot be used in WB to detect AChE. Not suitable for Flow. Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB ] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in U251 Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

3 Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB ] - Both normal and cancer biopsies of deparaffinized human Cerebellum tissue. Page 2 of 5 v.20.1 Updated 3/19/2018 Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB ] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in Hela Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated. Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HR2) [NB ] - Analysis of Acetylcholinesterase using Anti-Acetylcholinesterase Monoclonal Antibody (HR2) shows staining in Neuro-2a Cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated. Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB ] - Both normal and cancer biopsies of deparaffinized human Rectum tissue.

4 Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HR2) [NB ] - Both normal and cancer biopsies of deparaffinized human Brain tissue. Page 3 of 5 v.20.1 Updated 3/19/2018 Publications Zeineh MM, Chen Y, Kitzler HH et al. Activated iron-containing microglia in the human hippocampus identified by magnetic resonance imaging in Alzheimer disease. Neurobiol Aging 2015 Sep [PMID: ] (Human)

5 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NBL HAF007 NB720-B NBP Acetylcholinesterase/ACHE Overexpression Lysate (Native) Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] (Preabsorbed) Mouse IgG2b Isotype Control (MPC-11) Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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