CATALOG 2012/2013. BioTek Microplate Instrumentation Catalog 2012/2013. Wash. Read. Automate. Dispense

Transcription

1 BioTek Microplate Instrmentation Catalog 2012/2013 CATALOG 2012/2013 Read Wash Dispense Atomate

2 Are yo reading, washing, dispensing or atomating a microplate-based process? If so, we have the instrmentation and expertise to help. BioTek Instrments, Inc., headqartered in Winooski, VT, USA, is a worldwide leader in the design, manfactre, and sale of microplate instrmentation and software. BioTek instrmentation is sed to aid in the advancement of life science research, facilitate the drg discovery process, provide rapid and cost-effective indstrial analysis and to enable sensitive and accrate qantification of a wide range of molecles across diverse applications. Or company-wide commitment to qality and vale is backed by sperior cstomer care, technical service centers, scientific application experts, and a knowledgeable sales force. Or commitment and focs helps to ensre yor processes will be rapid, efficient and sccessfl. This catalog provides an overview of or complete line of microplate instrmentation. For more detailed information visit or web site at BioTek is headqartered in Vermont, USA with global sales, service and distribtion spport. BioTek is ISO9001/ ISO13485 Certified, an FDA Registered Medical Device Manfactrer, and has appropriate prodcts in compliance with the EU In Vitro Diagnostic Directive (IVD). Reglatory compliance is ensred with both validation (IQ/OQ/PQ) and 2 FDA 21 CFR Part 11 tools.

3 R E A D NEW W A S H Overview... 4 Synergy NEO... 6 Synergy H Synergy H1 (new option) Synergy Mx Synergy Synergy HT Synergy Reader Comparison Chart FLx PowerWave Eon Epoch ELx ELx Absorbance Reader Comparison Chart Gen5 Data Analysis Software Take3 Micro-Volme Plate Reader Accessories Overview EL NEW 405 Toch NEW 405 LS ELx405 Select Deep Well ELx Washer Comparison Chart Liqid Handling Control Secre Software Washer Accessories D I S P E N S E Overview MltiFlo MicroFill Dispenser Comparison Chart Liqid Handling Control Software Dispenser Accessories P I P E T T E Overview Precision A U T O M A T E Overview NEW BioStack Compliance Applications Spport Global Service and Spport Year History Contact Information

4 4 BioTek Readers Overview

5 Read BioTek offers an extensive range of microplate readers, from the Synergy NEO HTS Mlti-Mode Microplate Reader to the ELx800, a basic ELISA reader sed in tens of thosands of laboratories arond the globe. Inclded in the BioTek reader prodct range are Hybrid readers, mlti-mode readers, florometers, lminometers and a variety of both monochromator-based spectrophotometers and filter-based absorbance readers. For assays like ncleic acid and protein qantification, where very small sample size is critical, BioTek s Take3 Micro-Volme Plate offers the ability to measre mltiple samples as small as 2 µl in monochromator-based mlti-mode and absorbance readers. Many microplate readers come with the powerfl Gen5 Data Analysis Software and are compatible with BioStack atomation prodcts and many third-party integrators, to provide increased throghpt and nattended operation. 5

6 Synergy NEO HTS Mlti-Mode Microplate Reader Synergy NEO is an HTS mltimode microplate reader designed specifically for today s screening and core laboratories. NEO is BioTek s newest advancement in detection technology; it has all the featres expected in an HTS screening instrment, inclding mltiple parallel detectors for ltra-fast measrements, a dedicated filter-based system for live cell assays, and exqisite performance. High Throghpt Synergy NEO can read a 96-well plate in 6 seconds and a 384-well plate in 11 seconds. With a load/ nload plate transfer time of abot 6 seconds per plate, NEO s optional integrated plate stacker makes this the fastest combination on the market, allowing walk-away atomation of short- or long-term assays. Hybrid Technology Optical System Synergy NEO s core is the patent pending Hybrid design that incorporates monochromatorbased and filter-based systems for the tmost flexibility and performance in HTS, research and drg discovery laboratories. Assay Versatility, Alpha Capability Synergy NEO meets today s diverse assay reqirements by incorporating featres like a powerfl 100 mw laser-based excitation sorce for Alpha-based assays (AlphaScreen, AlphaLISA, SreFire ). The Alpha capability of NEO provides speed and sensitivity even for low-signal cellbased assays. Other featres, sch as advanced temperatre control to 65 C, orbital and linear shaking rond ot the powerhose of fnctionality and performance that defines the NEO. Designed for Atomation Ease of se and ser confidence are key to the design of Synergy NEO s niqe filter cbes that are barcode labeled for positive filter ID limiting the possibility of errors and streamlining the workflow. An available camera-based barcode reader can scan both 1D and 2D barcode-labeled plates. Gen5 Software Control The otstanding fnctionality of Synergy NEO is controlled by the powerfl, yet easy-to-se, Gen5 Data Analysis Software. Gen5 s nclttered interface and completely cstomizable otpt options make it an all-inone soltion for any NEO assay reqirement. Typical Applications: XXHTS screening XXDrg absorption and metabolism XXBiologics drg discovery and development XXDrg discovery XXCell proliferation XXCytotoxicity XXBiomarker qantification XXGenetic analysis XXEnvironmental testing XXFood safety XXNcleic acid qantification XXProtein qantification Synergy NEO shown with optional high-speed microplate stacker 6 BioTek Readers Synergy NEO

7 Specifications General Wavelength selection Detection method Read mode Microplate types Temperatre control Shaking Read height Kinetic speed With Stacker, minimm processing time per plate Barcode reader Software Absorbance Light sorce Wavelength selection Wavelength range Bandpass Dynamic range Resoltion Hybrid Technology (patent pending) Qadrple Monochromators and Filter Cbes Florescence, Time-Resolved Florescence, Florescence Polarization, AlphaScreen /AlphaLISA, Lminescence, UV-Visible Absorbance End point, kinetic, spectral scanning, well-area scanning 1- to 1536-well plates Compatible with Take3 Micro-Volme Plates 3º C above ambient to 65º C ±0.5º C at 37º C Linear, orbital, doble orbital Ato Z, 0.1 mm steps, top/bottom (filters), top (mono) 96 well: 6 seconds 384 well: 11 seconds 1536 well: 25 seconds 96 well: 20 seconds 384 well: 25 seconds 1536 well: 39 seconds Mlti-directional, 2D camera-based Gen5 Data Analysis Software Xenon flash lamp Monochromator nm, 1 nm increment 2 nm ( nm), 4 nm (>285 nm) OD OD Florescence Intensity Light sorce Sensitivity Wavelength selection Wavelength range Bandpass Detection system Xenon flash lamp Qadrple monochromator: Florescein 2 pm typical - Top Florescein 2.5 pm typical - Bottom Filter cbes: Florescein 0.5 pm typical - Top Florescein 1 pm typical - Bottom Doble grating monochromators (top/bottom) and, Filter cbes (top/bottom) Monochromators: nm Filters cbes: nm Monochromators: 16 nm excitation / emission Filters: filter-dependent, from 5 nm to >100 nm Two matched PMTs top filter system Low noise PMT bottom filter system Red shifted PMT top/bottom monochromator system Lminescence Sensitivity Wavelength range Dynamic range 5 amol ATP typical (flash) nm >6 decades Florescence Polarization Light sorce Sensitivity Wavelength selection Wavelength range Xenon flash 1 mp at 1 nm florescein typical Filter cbes (top / bottom) nm Time-Resolved Florescence Light sorce Sensitivity Wavelength selection Wavelength range Alpha Light sorce Sensitivity Wavelength selection Read speed Reagent Dispensers Nmber Dispense volme Dead volme Plate geometry Xenon flash Eropim 40 fm typical (384-well low volme plate) Filter cbes (top/bottom) Doble grating monochromator (top / bottom) Filters: nm Monochromators: nm 100 mw 680 nm laser 100 amol LCK peptide Filter cbes 96 well: 30 seconds 384 well: 1 minte, 50 seconds 1536 well: 7 mintes, 20 seconds 2 syringe pmps µl in 1 µl increments 1.1 ml, 100 µl with back flsh 6- to 384-well microplates Dispense precision <2% at µl Dispense accracy ±1 µl or 2% Physical Characteristics Power Dimensions Weight Reglatory 250 Watts max W x 20.7 D x 16.1 H (39 x 52.5 x 41 cm) 78 lbs (35 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 7

8 Synergy H4 Hybrid Mlti-Mode Microplate Reader Synergy H4 is BioTek s most versatile mlti-mode reader, and part of BioTek s patent pending Hybrid reader category, combining monochromator and filter-based wavelength selection in one instrment. Its niqe featres inclde wavelength scanning, variable bandpass selection for increased flexibility, extended incbation range to 65ºC for high-temperatre assays and Alpha detection mode. An optional reagent injection system is available. All Yo Need in One Compact Instrment Synergy H4 covers the broadest range of applications. Eqipped with two main optical systems (monochromator-based and filter- based) and 6 major detection modes (UV-visible absorbance, top/bottom florescence, lminescence, FP, TRF and Alpha) it can rn virtally any microplatebased assay from basic research assays to high throghpt drg discovery assays. Hybrid Technology : Sensitivity and Flexibility Hybrid Technology is BioTek s patent pending design that combines two different optical systems in one compact instrment. This design makes Synergy H4 the ideal instrment for research and drg discovery applications when having to choose between flexibility and performance is not an option. Alpha Detection Mode Alpha-based assays (AlphaScreen, AlphaLISA, SreFire ) are growing in poplarity becase of the workflow simplicity (homogeneos, no-wash assays), and the level of sensitivity they provide for celllar assays. With its niqe optical system, Synergy H4 is one of few readers in its category to provide highperformance detection for Alphabased assays. Typical Applications: Ncleic acid qantification Protein qantification Enzyme kinetics Biomarker qantification ELISAs Genetic analysis Drg discovery Cell proliferation Cytotoxicity Drg absorption and metabolism Biologics drg discovery and development Food safety Biofels research Environmental monitoring 8 BioTek Readers Synergy H4

9 Specifications General Wavelength selection Hybrid Technology (patent pending) Qadrple Monochromators and Filters / Dichroics Florescence Intensity Light sorce Tngsten halogen lamp High energy Xenon flash lamp Detection method Read method Microplate types Temperatre control Shaking Florescence, Time-Resolved Florescence, Florescence Polarization, AlphaScreen/AlphaLISA, Lminescence, UV-Visible Absorbance End point, kinetic, spectral scanning, well-area scanning Monochromator system 1- to 384-wells Filter system 1- to 1536-wells PCR plates Compatible with Take3 Micro-Volme Plate 4 C above ambient to 65 C; ±0.5 C at 37 C Yes Sensitivity Wavelength selection Wavelength range Dynamic range Monochromators: Top: Florescein 2 pm typical (0.2 fmol/well 384-well plate) Bottom: Florescein 2.5 pm typical (0.25 fmol/well 384- well plate) Filters/mirrors: Florescein 1 pm typical (0.1 fmol/well 384-well plate) Doble grating monochromators (Top/Bottom) and Deep blocking filters / dichroic mirrors (Top) Monochromators: nm Filters: nm (850 nm option) Monochromators: 5 decades Filters/mirrors: >6 decades Software Atomation AlphaScreen/AlphaLISA Light sorce Sensitivity Dynamic range Reading speed Detection system Absorbance Light sorce Wavelength selection Gen5 Data Analysis Software Compatible with BioStack and 3rd party atomation Tngsten halogen lamp 100 amol of biotinylated-lck-p peptide, 25 µl/well in 384-well plate >6 decades 2 mintes typical for 96-well plate Ultra low noise PMT Xenon flash lamp Monochromator Bandpass Detection system Reading speed Lminescence Sensitivity Wavelength range Dynamic range Monochromators: Variable 9, 13.5, 17, 20 nm Filters: Filter-dependant, from 5 nm to >100 nm Red shifted PMT for monochromator system Low noise PMT for filter-based system 96 wells: 11 seconds 384 wells: 22 seconds 1536 wells: 42 seconds 10 amol ATP typical (flash) nm >6 decades Florescence Polarization Light sorce Sensitivity Tngsten halogen 3 mp at 1 nm florescein typical Wavelength range Bandpass Dynamic range Resoltion Pathlength correction Monochromator wavelength accracy Monochromator wavelength repeatability OD accracy OD linearity OD repeatability Stray light Reading speed nm, 1 nm increments 2 nm ( nm), 4 nm (>285 nm) OD OD Yes ±2 nm ±0.2 nm <1% at 2.0 OD typical <3% at 3.0 OD typical <1% from 0 to 3.0 OD typical <0.5% at 2.0 OD typical 0.03% at 230 nm typical 96 wells: 11 seconds 384 wells: 22 seconds Wavelength selection Wavelength range Time-Resolved Florescence Light sorce Sensitivity Wavelength selection Wavelength range Reagent Dispensers Nmber Dispense volme Minimm prime volme Physical Characteristics Deep blocking filters / dichroic mirrors (Top) nm ( nm option) High energy Xenon flash lamp Eropim 60 fm typical with filters (6 amol/well in 384-well plate) Deep blocking filters / dichroic mirrors (Top) Doble grating monochromator (Top / Bottom) Filters: nm (850 nm option) Monochromators: nm 2 syringe pmps µl in 1 µl increment 1.1 ml, 100 µl with back flsh Power Volts AC 50/60 Hz Specifications are sbject to change. Dimensions Weight Reglatory 17 W x 20.9 D x 15 H (43.5 x 53.1 x 38.1 cm) 78 lbs (35 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. 9

10 Synergy H1 Hybrid Mlti-Mode Microplate Reader Synergy H1 is part of BioTek s Hybrid reader category; eqipped with both monochromator and filter optical systems. Synergy H1 provides the same combination of flexibility and performance as the Synergy H4, at a very attractive price. An optional reagent injection system is available. Flexibility at a Great Price Synergy H1 is available in a monochromator-only configration. Spporting top and bottom florescence, UV-visible absorbance and lminescence, it is the most cost-effective soltion of its type on the market. Combined with the Take3 Micro- Volme Plate for low volme 2 µl assays, it is the perfect instrment for life-science research laboratories. Patent pending Hybrid Optical System The optional filter modle can trn the Synergy H1 into an advanced Hybrid reader. This patent pending optical design is only available from BioTek. Monochromators provide easeof-se and flexibility, while filters provide increased optical efficiency and sensitivity. NEW! Gas Controller for Live Cell Assays The new Gas Controller for Synergy H1 allows control and monitoring of CO 2 and O 2 levels in the system. The Gas Controller, along with advanced temperatre control to 65 C and orbital shaking, create the ideal physiological environment needed for assays sing live cells. Upgradeable to Advanced Read Modes When eqipped with the optional filter modle, Synergy H1 may be sed for florescence polarization assays as well as Time-Resolved Florescence (TRF) and TR-FRET assays. Typical Applications: Ncleic acid qantification Protein qantification Enzyme kinetics Biomarker qantification ELISAs Yeast kinetic analysis Genetic analysis Drg discovery Cell proliferation Cytotoxicity Drg absorption and metabolism Biologics drg discovery and development Food safety Environmental monitoring Synergy H1 shown with optional Gas Controller modle 10 BioTek Readers Synergy H1

11 Specifications General Wavelength selection Detection method Read method Microplate types Hybrid Technology (patent pending) Qadrple Monochromators and Filters / Dichroics Monochromator system: Florescence, Lminescence, UV-Visible Absorbance Filter system: Florescence, Time-Resolved Florescence, Florescence Polarization, Lminescence End point, kinetic, spectral scanning, well-area scanning 1- to 384-wells Compatible with Take3 Micro-Volme Plate Florescence Intensity Light sorce Sensitivity Wavelength selection Xenon flash lamp Monochromators: Top: Florescein 2.5 pm typical (0.25 fmol/well 384-well plate) Bottom: Florescein 5 pm typical (0.5 fmol/well 384-well plate) Filters/mirrors: Florescein 1 pm typical (0.1 fmol/well 384-well plate) Doble grating monochromators (Top and Bottom) and, Deep blocking bandpass filters / dichroic mirrors (Top) Temperatre control Shaking Software Atomation CO 2 and O 2 control Absorbance Light sorce Wavelength selection Wavelength range Bandpass Dynamic range Resoltion Pathlength correction Monochromator wavelength accracy Monochromator wavelength repeatability OD accracy OD linearity OD repeatability Stray light Reading speed To 45 C; ±0.5 C at 37 C Yes Gen5 Data Analysis Software Compatible with BioStack and 3rd party atomation With optional Gas Controller Xenon flash lamp Monochromator nm, 1 nm increment 4 nm ( nm), 8 nm (>285nm) OD OD Yes ±2 nm ±0.2 nm <1% at 2.0 OD typical <3% at 3.0 OD typical <1% from 0 to 3.0 OD typical <0.5% at 2.0 OD typical 0.03% at 230 nm typical 96 wells: 11 seconds 384 wells: 22 seconds Specifications are sbject to change. Wavelength range Dynamic range Detection system Reading speed Lminescence Sensitivity Wavelength range Dynamic range nm (850 nm option) 5 decades Two PMT detectors: one for monochromator system, one for filter system 96 wells: 11 seconds 384 wells: 22 seconds Monochromator system: 20 amol ATP typical (flash) Filter system: 10 amol ATP typical (flash) nm >6 decades Florescence Polarization Sensitivity Wavelength range Time-Resolved Florescence Light sorce Sensitivity Wavelength range Reagent Dispensers 5 mp at 1 nm florescein typical nm ( nm option) Xenon flash lamp Eropim 100 fm typical with filters (10 amol/well in 384-well plate) nm (850 nm option) Dispense precision <2% at µl Dispense accracy ±1 µl or 2% Nmber Plate geometry Dispense volme Minimm prime volme Physical Characteristics 2 syringe pmps 1- to 384-well microplates µl in 1 µl increments 1 ml, 100 µl with back flsh Power Volts AC 50/60 Hz Dimensions 15.4"W 18.6"D 12.9"H (39.1 x 47.2 x 32.8 cm) Weight Reglatory 50 lbs (22.5 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. 11

12 Synergy Mx Mlti-Mode Microplate Reader Monochromator-based instrments provide ease of se and flexibility, covering a broad range of applications ot of the box. Synergy Mx can be eqipped with the following modlar options: UV-visible absorbance, top florescence, bottom florescence, lminescence, and cvette port and reagent injection system. Ultra Fine-Tned Performance The Synergy Mx is flly monochromator-based, providing wavelength selection with a qadrple grating architectre for ncompromised flexibility. Any assay from the low UV to the near infrared may be atomated on this reader, withot ever needing a new filter. Qadrple Monochromator Optics Both excitation and emission monochromators se a dal grating architectre. This design provides a very niform performance across the spectrm, withot jmps associated with filter-grating designs. Variable Bandpass Selection System Each monochromator is eqipped with for software-selectable slits to vary the excitation and emission bandpass. This adds flexibility to the system: a narrow band pass can be sed for mltiplexed assays, and wider bandpass can be sed to maximize sensitivity. Cvette Port and Temperatre Control to 65ºC These additional featres and options expand the application range of the instrment. The cvette port allows rapid analysis of a few samples, and the extended incbation range enables high-temperatre assays. Typical Applications: Ncleic acid qantification Protein qantification Enzyme kinetics Biomarker qantification ELISAs Genetic analysis Drg absorption and metabolism Cell proliferation Cytotoxicity Food safety Environmental monitoring 12 BioTek Readers Synergy Mx

13 Specifications General Detection method Read method Microplate types Temperatre control Shaking Software Atomation Absorbance Light sorce Wavelength selection Wavelength range Bandpass Dynamic range Resoltion Pathlength correction Monochromator wavelength accracy Monochromator wavelength repeatability OD accracy OD linearity OD repeatability Reading speed Florescence, UV-Visible Absorbance, Lminescence, Time-Resolved Florescence (secondary mode) End point, kinetic, spectral scanning, well-area scanning 1- to 384-wells PCR plates Compatible with Take3 Micro-Volme Plate 4 C above ambient to 65 C; ±0.5 C at 37 C Yes Gen5 Data Analysis Software Compatible with BioStack and 3rd party atomation High energy Xenon flash Doble monochromator nm, 1 nm increment 2 nm ( nm), 4 nm (>285 nm) OD OD Yes ±2 nm ±0.2 nm <1% at 2.0 OD typical <1% from 0 to 3.0 OD typical <0.5% at 2.0 OD typical 96 wells: 11 seconds 384 wells: 22 seconds Florescence Intensity Light sorce Sensitivity Wavelength selection Wavelength range Dynamic range Bandpass Detection system Reading speed Lminescence Sensitivity Wavelength range Dynamic range Detection system Reagent Dispensers Nmber Dispense volme Minimm prime volme High energy Xenon flash Top: Florescein 2 pm typical (0.2 fmol/well 384-well plate) Bottom: Florescein 2.5 pm typical (0.25 fmol/well 384-well plate) Doble grating monochromators (Top and Bottom) nm 5 decades Variable: 9, 13.5, 17, 20 nm Dedicated red shifted PMT 96 wells: 11 seconds 384 wells: 22 seconds 10 amol ATP typical (flash) nm >6 decades Physical Characteristics Low noise PMT 2 syringe pmps µl in 1 µl increment 1.1 ml, 100 µl with back flsh Power Volts AC 50/60 Hz Dimensions Weight Reglatory 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm) 70 lbs (31.5 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 13

14 Synergy 2 Mlti-Mode Microplate Reader The Synergy 2 has been designed for life science research and drg discovery applications. It incorporates enhanced florescence, lminescence and absorbance optics for sperior performance. Advanced read modes sch as florescence polarization and time-resolved florescence are available as individal, pgradeable modles. An optional reagent injection system is available. Best Price / Performance Ratio The Synergy 2 incorporates dedicated, optimized optical paths for each detection mode sing filters for florescence and a monochromator for absorbance. The reslt is excellent performance in all modes, at an attractive price. Sensitive Dichroic-based Florescence Optics The Synergy 2 florescence optics are a step p from the Synergy HT, incorporating dichroic mirrors, which decrease backgrond noise, as well as a liqid-filled emission fiber that increases the system s light collection efficiency. The reslt is higher sensitivity for demanding assays. Dedicated Lminescence Light Path A dedicated liqid-filled light gide copled with a low noise detector provides highperformance lminescence detection, on par with dedicated microplate lminometers. Synergy 2 is DLReady certified by Promega to rn their Dal- Lciferase assay system. Advanced, Modlar Read Modes In addition to the basic read modes available on the Synergy HT, Synergy 2 offers florescence polarization, time-resolved florescence and Alpha detection modes, available as individal, pgradeable modles. Typical Applications: Ncleic acid qantification Protein qantification Enzyme kinetics Biomarker qantification ELISAs Genetic analysis Drg discovery Cell proliferation Cytotoxicity Drg absorption and metabolism Biologics drg discovery and development Food safety Biofels research Environmental monitoring 14 BioTek Readers Synergy 2

15 Specifications General Detection method Read method Microplate types Temperatre control Shaking Software Atomation Florescence, Time-Resolved Florescence, Florescence Polarization, AlphaScreen /AlphaLISA, Lminescence, UV-Visible Absorbance End point, kinetic, spectral scanning, well-area scanning 6- to 1536-wells (lminescence 1- to 384-wells) PCR plates Compatible with Take3 Micro-Volme Plate 4 C above ambient to 65 C; ±0.5 C at 37 C Yes AlphaScreen/AlphaLISA Light sorce Sensitivity Dynamic range Reading speed Detection system Absorbance Light sorce Wavelength selection Wavelength range Bandpass Dynamic range Resoltion Pathlength correction Monochromator wavelength accracy Monochromator wavelength repeatability OD accracy OD linearity OD repeatability Stray light Reading speed Gen5 Data Analysis Software Compatible with BioStack and 3rd party atomation Tngsten halogen lamp 100 amol of biotinylated-lck-p peptide, 25 µl/well in 384-well plate >6 decades 2 mintes typical for 96-well plate Ultra low noise PMT SQ Xenon flash lamp Monochromator nm, 1 nm increment 2.4 nm OD OD Yes ±2 nm ±0.2 nm <1% at 2.0 OD typical <3% at 3.0 OD typical <1% from 0 to 3.0 OD typical <0.5% at 2.0 OD typical 0.03% at 230 nm typical 96 wells: 11 seconds 384 wells: 22 seconds 1536 wells: 42 seconds Florescence Intensity Light sorce Sensitivity Wavelength selection Wavelength range Dynamic range Bandpass Detection system Reading speed Lminescence Sensitivity Wavelength range Dynamic range Detection system Tngsten halogen High energy DPR Xenon flash (optional) Top: Florescein 1 pm typical (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate) Bottom: Florescein 5 pm typical (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) Deep blocking bandpass filters/dichroic mirrors nm with Tngsten lamp (850 nm option) nm with Xenon lamp (850 nm option) >6 decades Filter dependent PMT 96 wells: 11 seconds 384 wells: 22 seconds 1536 wells: 42 seconds 10 amol ATP typical (flash) nm >6 decades Low noise PMT Florescence Polarization Light sorce Sensitivity Wavelength selection Wavelength range Time-Resolved Florescence Light sorce Sensitivity Wavelength selection Wavelength range Reagent Dispensers Nmber Dispense volme Minimm prime volme Physical Characteristics Tngsten halogen High energy DPR Xenon flash (optional) 3 mp at 1 nm florescein typical Deep blocking filters / dichroic mirrors nm ( nm option) High energy DPR Xenon flash Eropim 60 fm typical (12 amol/well 96-well plate; 6 amol/ well 384-well plate) Deep blocking filters/mirrors nm (850 nm option) 2 syringe pmps µl in 1 µl increments 1.1 ml, 100 µl with backflsh Power Volts AC 50/60 Hz Dimensions 17 W x 17.5 D x 14.5 H (43.5 x 44.5 x 37.3 cm) Specifications are sbject to change. Weight Reglatory 60 lbs (27 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. 15

16 Synergy HT Mlti-Mode Microplate Reader The Synergy HT is an entry-level mlti-mode microplate reader sed in thosands of laboratories worldwide. All models come eqipped with top and bottom florescence, UV-visible absorbance and lminescence detection. Temperatre control to 50ºC, shaking and advanced data analysis software are also inclded. An optional reagent injection system is available. Ideal for Basic Research Applications The Synergy HT is the ideal instrment for ncleic acid and protein qantification, enzyme assays, biomarker qantification and ELISA assays, as well as cellbased assays (gene expression, celllar growth, cytotoxicity). Sensitive Filter-based Florescence For excitation and for emission filters are inclded with the reader, and can be sed for top and bottom reading. Bottom reading is sally recommended when working with adherent cells, as it often provides better signal-tobackgrond ratios. Top reading is sally best for assays where the florescence signal comes from the soltion. Flexible Monochromator-based Absorbance All Synergy readers se monochromators for absorbance detection. This provides nlimited wavelength selection from the low UV to the near infrared, in 1 nm steps, and enables spectral scanning. Low-noise Lminescence Detection The Synergy HT can atomate glow and flash lminescent assays, thanks to its optional injection system. Typical assays inclde ATP qantification as well as lciferase gene expression assays. Typical Applications: Ncleic acid qantification Protein qantification Enzyme kinetics Biomarker qantification ELISAs Genetic analysis Cell proliferation Cytotoxicity Drg absorption and metabolism Food safety Environmental monitoring 16 BioTek Readers Synergy HT

17 Specifications General Detection method Florescence, Time-Resolved Florescence (secondary mode), Lminescence, UV-Visible Absorbance Florescence Intensity Light sorce Tngsten halogen SQ Xenon flash Read method Microplate types End point, kinetic, spectral scanning, well-area scanning 6- to 384-wells PCR plates Compatible with Take3 Micro-Volme Plate Sensitivity Wavelength selection Top and Bottom: Florescein 5 pm typical (1 fmol/well 96-well plate) Deep blocking filters Temperatre control Shaking Software 4 C above ambient to 50 C; ±0.5 C at 37 C Yes Gen5 Data Analysis Software Wavelength range Dynamic range Bandpass Detection system nm ( nm option) 5 decades Filter dependent PMT Atomation Absorbance Light sorce Wavelength selection Compatible with BioStack and 3rd party atomation SQ Xenon flash Monochromator Reading speed Lminescence Sensitivity Wavelength range 96 wells: 31 seconds 384 wells: 80 seconds 30 amol ATP typical (flash) nm Wavelength range nm, 1 nm increments Dynamic range >6 decades Bandpass Dynamic range Resoltion Pathlength correction Monochromator wavelength accracy Monochromator wavelength repeatability OD accracy OD linearity OD repeatability Stray light Reading speed 2.4 nm OD OD Yes ±2 nm ±0.2 nm <1% at 2.0 OD typical <3% at 3.0 OD typical <1% from 0 to 3.0 OD typical <0.5% at 2.0 OD typical 0.03% at 230 nm typical 96 wells: 14 seconds 384 wells: 26 seconds Detection system Low noise PMT Time-Resolved Florescence (Secondary Mode) Light sorce Wavelength selection Reagent Dispensers Nmber Dispense volme Minimm prime volme Physical Characteristics SQ Xenon flash (optional) Monochromator 2 syringe pmps µl in 1 µl increments 1.1 ml, 100 µl with backflsh Power Volts AC 50/60 Hz Dimensions Weight Reglatory 16"W x 15"D x 10"H (40.6 x 38 x 25.4 cm) 40 lbs (18 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 17

18 Synergy Reader Comparison Chart Which Synergy Reader is right for yo? Key Featres HTS Readers Synergy NEO Speed/Performance Synergy H4 Performance Hybrid Technology Synergy H1 Monochromator-based UV-visible absorbance Florescence top/bottom Lminescence Filtered lminescence Injectors TRF & TR-FRET Florescence polarization Standard AlphaLISA / AlphaScreen HTS Laser AlphaLISA / AlphaScreen Hybrid Technology Atomation ready/biostack compatible Dal PMT read head Performance Specifications Florescein typical top 0.5 pm 1 pm / 2 pm 1 pm / 2.5 pm Florescein typical bottom 1 pm 2.5 pm 5 pm ATP typical flash lminescence 5 amol 10 amol 10 amol Polarization typical 1 nm Florescein 1 mp 3 mp 5 mp Eropim typical 40 fm 60 fm 100 fm AlphaScreen typical 100 amol 100 amol n/a Fastest read speed 96-/384-well plates 6/11 11/22 11/22 General Specifications Microplate types 1 to to to 384 Gas Controller compatible Barcode reader option Take3 Micro-Volme Plate compatible Cvette port option Temperatre control system to 65ºC to 65ºC to 45ºC Filter capacity 6 filter sets 4 filter sets 2 filter sets Florescence bandpass Filter dependent mono 16 nm Filter dependent mono variable: 9, 13.5, 17, 20 nm Filter dependent mono 16 nm Vale 18 BioTek Readers Synergy Reader Comparison Chart

19 Monochromator-based Filter-based Synergy Mx Synergy H1m Synergy 2 Synergy HT Performance Vale Performance Vale (secondary mode) (secondary mode) (secondary mode) 2 pm 2.5 pm 1 pm 5 pm 2.5 pm 5 pm 5 pm 5 pm 10 amol 20 amol 10 amol 30 amol n/a n/a 3 mp n/a n/a n/a 60 fm n/a n/a n/a 100 amol n/a 11/22 11/22 11/22 14/26 1 to to to to 384 to 65ºC to 45ºC to 65ºC to 50ºC n/a n/a 4 filter sets 4 filter sets Variable: 9, 13.5, 17, 20 nm 16 nm Filter dependent Filter dependent 19

20 FLx800 Florescence Microplate Reader The compact FLx800 florescence reader provides high performance in 6- to 384-well microplates at an attractive price. Options inclde top and bottom detection, temperatre control and reagent injector. Great Price/Performance Ratio The FLx800 ses the same florescence optical system as the Synergy HT: top and bottom bifrcated qartz fibers ensre strong sample excitation and efficient collection of the emitted signal. This reader combines sensitivity, convenience and ease of se all at a great price. Specifications General Detection method Florescence, Lminescence Bottom Reading and FRET Detection The bottom detection system ses a large 5 mm diameter qartz fiber optimized for cell-based assays in 96-well plates and smaller densities. The reader may be eqipped with p to 4 filter sets and may be sed to rn cell-based FRET assays. Read method Microplate types Temperatre control Shaking Software Onboard software Florescence Intensity End point, kinetic and area scanning nder compter control 6- to 384-wells PCR plates (FLx800TBP) 4 C above ambient to 50 C (I models) Yes (I models) Gen5 Data Analysis Software 55 ser-programmable protocols Sensitive Lminescence Detection The FLx800 s detector provides very high sensitivity when rnning lminescent assays. ATP or lciferase can be qantified down to very low concentrations sing the reader s photon integration mode. Light sorce Sensitivity Wavelength selection Wavelength range Dynamic range Lminescence Sensitivity Tngsten halogen Florescein 5 pm typical (1 fmol/well 96-well plate) Deep blocking filters nm (850 nm option) 5 decades 100 amol ATP typical (flash) Florescent Ion Channel Assays A syringe pmp injector is available as an option to atomate florescent ion-channel assays. This system is sed to inject a trigger reagent that indces a fast change in florescent signal. The FLx800 kinetically monitors the signal jst after injection. Typical Applications: Ncleic acid qantification Protein qantification Enzyme kinetics Genetic analysis by florescence Celllar analysis by florescence Wavelength range Dynamic range Reagent Dispensers Nmber Dispense volme Minimm prime volme Physical Characteristics nm 5 decades 1 syringe pmp µl in 1 µl increments 1.1 ml, 100 µl with backflsh Power Volts AC 50/60 Hz Dimensions Weight Reglatory 15"W x 16"D x 9"H (38.1 x x cm) 30 lbs (13.6 kg) Specifications are sbject to change. For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. 20 BioTek Readers FLx800

21 PowerWave Microplate Spectrophotometer The PowerWave HT is a high throghpt, robot friendly microplate spectrophotometer with a very small footprint, ideal for integration into atomated systems. Wavelength selection in 1 nm increments, temperatre control and sperior performance p to 4.0 OD add to its appeal for a variety of assay needs. High Speed, Higher Throghpt In atomation and high throghpt, timing is everything With 8 reading channels, the PowerWave HT can read a 96-well plate in 5 seconds. Low Stray Light Monochromator Optics PowerWave HT s monochromator optics pre-select the measrement wavelength before light goes throgh the sample. This reslts in very low stray light reaching the detector with the added benefit of excellent performance even at high optical densities. Specifications General Detection method Read method Microplate types Absorbance End point, kinetic, spectral, linear scanning 96- and 384-wells Temperatre control 4 C above ambient to 50 C; ±0.5 C at 37 C Shaking Software Atomation Absorbance Light sorce Wavelength selection Wavelength range Yes Gen5 Data Analysis Software Compatible with BioStack and 3rd party atomation Xenon flash lamp Monochromator, selectable 1 nm or greater increments nm BioStack Compatible for Benchtop Atomation When walkaway benchtop atomation is reqired, the PowerWave HT, copled with BioStack, provides a compact system for rapid processing of p to 50 plates at a time. Gen5 Control = Assay Flexibility Bandpass Dynamic range Resoltion Monochromator wavelength accracy Monochromator wavelength repeatability OD accracy OD linearity 5 nm OD OD ±2 nm ±0.2 nm 1% ±0.01 OD typical ±1% typical Gen5 Data Analysis Software not only allows easy control of all the fnctionality of the PowerWave HT, it also spports a vast nmber of applications in absorbance. Qick export to Microsoft Excel or se Gen5 s powerfl data analysis tools to make qick work of the most complex assays. Typical Applications: Enzyme kinetics ELISAs Genetic analysis by colorimetry Celllar analysis by colorimetry Cell proliferation OD repeatability Stray light Reading speed Physical Characteristics 0.5% ±0.005 OD typical 0.03% at 230 nm typical 96 wells: 5 seconds 384 wells: 11 seconds Power Volts AC 50/60 Hz Dimensions Weight Reglatory 8.5 W x 16"D x 8.5"H (21.6 x 40.6 x 21.6 cm) 24 lbs (11 kg) Specifications are sbject to change. For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. BioTek Readers PowerWave 21

22 Eon Microplate Spectrophotometer The Eon Microplate Spectrophotometer is the latest in BioTek s line of high performance, high vale microplate instrments. With its monochromator-based optics, extended incbation range, optimized shaking capability, and optional cvette port, Eon accommodates a wide range of assay applications in the life science laboratory, from DNA, RNA and protein qantification to long rnning kinetic assays. Filterless Wavelength Selection Eon s optical system allows wavelength selection between 200 and 999 nm in as little as 1 nm increments. No filters reqired for a new assay Eon has it covered. It s easy to view the sample s peak wavelengths jst rn a qick spectral scan in the cvette, or in a microplate well. Mltiple Shake Modes For cell assays that need gentle orbital shaking, or samples that need a vigoros shake, Eon has all the options. Choose from orbital, doble orbital or linear shaking. Shaking freqency and dration are all cstomizable for easy assay optimization. Sperior, Advanced Incbation Eon offers BioTek s innovative 4-Zone temperatre control system and takes it p a notch to 65 C. The higher setpoint capability expands application possibilities withot the need for other instrmentation. Micro-Volme, Microplate, Cvette Measrements Eon offers a standard cvette port option, and is compatible with the Take3 and Take3 Trio. With these options, Eon becomes a mltitasking workstation for microvolme ncleic acid or protein qantification, qick cvette measrements or any assay in a 6- to 384-well microplate. Atomation Ready Pair the Eon with BioStack for compact, convenient, walk-away atomation. When more complex rotines reqire integration into a cstom atomated system, Eon is ideally sited to the reqirement. BioTek works closely with atomation providers to offer rapid and seamless integration. Typical Applications: Ncleic acid qantification Protein qantification Micro-volme assays with Take3 Biofel research applications Cell proliferation Cytotoxicity ELISA Enzyme kinetics 22 BioTek Readers Eon

23 Specifications General Detection method Read method Microplate types Other labware Absorbance End point, kinetic, spectral scanning, well area scanning 6- to 384-wells Standard cvette (C model), BioCell and Take3 Micro-Volme Plate Temperatre control 4 C above ambient to 65 C; ±0.5 C at 37 C Shaking Software Options Atomation Absorbance Light sorce Wavelength selection Wavelength range Bandpass Dynamic range Resoltion Monochromator wavelength accracy Monochromator wavelength repeatability OD accracy OD linearity OD repeatability Orbital, doble orbital and linear Gen5 Data Analysis Software Cvette port Compatible with BioStack and 3rd party atomation Xenon flash lamp Monochromator, selectable in 1 nm or greater increments nm 2.4 nm OD OD ±2 nm (microplate and cvette measrements) ±0.2 nm (microplate and cvette measrements) 0.0 to 2.0 OD ±1% ±0.010 OD 2.0 to 3.0 OD ±3% ±0.010 OD (microplate and cvette measrements) 0.0 to 2.0 OD ±1% ±0.010 OD 2.0 to 3.0 OD ±3% ±0.010 OD (microplate and cvette measrements) 0.0 to 2.0 OD ±1% ±0.005 OD 2.0 to 3.0 OD ±3% ±0.005 OD (microplate and cvette measrements) Reading speed Physical Characteristics 96 wells: 8 seconds 384 wells: 13 seconds Power Volts AC 50/60 Hz Dimensions Weight Reglatory 8.5 W x 16 D x 8.5 H (21.6 x 40.6 x 21.6 cm) 24 lbs (11 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 23

24 Epoch Microplate Spectrophotometer Epoch is a monochromatorbased microplate spectrophotometer that offers sperior fnctionality for the life science laboratory at an accessible price. Controlled by the powerfl, yet easy-to-se Gen5 Data Analysis Software, Epoch is designed to be the new lab workhorse for a wide variety of applications. For walk-away atomation, an optional BioStack compatible Epoch is available. 200 nm to 999 nm Wavelength Range The monochromator-based optical system in Epoch allows any wavelength selection between 200 and 999 nm in 1 nm increments. No filters reqired! From low UV ncleic acid measrements to standard ELISA assays, Epoch is ideally sited to the life science laboratory where application flexibility is reqired. 6- to 384-well Microplate Reading Epoch s optical and mechanical systems are designed to provide optimal measrements in a variety of microplates. The area scanning capability provides mltiple measrements across larger diameter wells, reslting in more meaningfl data analysis. Take3 Micro-Volme Plate Compatible When sample size matters, as in critical ncleic acid and protein qantification, the Take3 plate provides p to sixteen 2 µl measrements withot needing to dilte important samples. End Point, Kinetic, Spectral Scanning There s no need to by expensive instrmentation to perform a variety of absorbance measrements. Epoch, driven by Gen5 Data Analysis Software, is the ltimate high-vale system with maximm assay flexibility. Typical Applications: Ncleic acid qantification Protein qantification 260/280 and 260/230 prity measrements ELISA Enzyme kinetics Cytotoxicity Cell proliferation Micro-volme assays with Take3 plate 24 BioTek Readers Epoch

25 Specifications General Detection method Read method Microplate types Other labware Software Atomation Absorbance Wavelength range Bandpass Dynamic range Absorbance End point, kinetic, spectral scan, well area scan 6- to 384-wells Take3 Micro-Volme Plate Gen5 Data Analysis Software Compatible with BioStack and 3rd party atomation (EpochR model) 200 nm nm, selectable in 1 nm increments 5 nm OD Resoltion Monochromator wavelength accracy OD accracy OD linearity OD repeatability ±2 nm 0 to 2 OD: ±1% ±0.010 OD 2 to 2.5 OD: ±3% ±0.010 OD 0 to 2 OD: ±1% ±0.010 OD 2 to 2.5 OD: ±3% ±0.010 OD 0 to 2 OD: ±1% ±0.005 OD 2 to 2.5 OD: ±3% ±0.005 OD Reading speed Physical Characteristics 96 wells: 15 seconds 384 wells: 31 seconds Power Volts AC 50/60 Hz Dimensions Weight Reglatory 12 W x 12.5 D x 7.7 H (30.5 cm x 31.8 cm x 19.6 cm) <15 lbs (6.8 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 25

26 ELx808 Absorbance Microplate Reader Excellent optical performance and sperior incbation are among the top featres of this mlti-channel reader. The ELx808 is sitable for a wide array of applications, from endpoint ELISAs to kinetic cell growth stdies. 4-Zone Temperatre Control For temperatre sensitive assays, there is no better incbation system in this microplate reader class than the ELx808. The natral convection heating is software controlled for consistency and performance over time. Specifications General Detection method Absorbance Read method End point, kinetic, linear scanning Fast Measrement The ELx808 can collect kinetic data in intervals as short as 6 seconds, for the most demanding assays. Gen5 Data Analysis Software provides mltiple kinetic and end point data analysis options for a variety of applications. Sperior Optical Performance The ELx808 can accommodate p to six absorbance filters, and its optical channels are staggered to prevent crosstalk between wells. The reference channel eliminates channel-to-channel variation. This niqe design gives the ELx808 its proven optical performance. Microplate types Temperatre control Shaking Software Onboard software Absorbance Light sorce Wavelength selection Wavelength range Bandpass Dynamic range Resoltion Filter wheel capacity Filters spplied OD accracy OD linearity OD repeatability 96-wells 4-Zone temperatre control to 50 C (ELx808IU) Yes Gen5 Data Analysis Software 55 ser-programmable protocols Tngsten halogen Filters nm nm (ELx808IU) 10 nm typical OD OD 6 positions 4 filters (5 with UV option) <1% at 2.5 OD typical <2% at 3.5 OD typical <1% at 2.5 OD typical <0.5% at 2.5 OD typical <1.5% at 3.5 OD typical Typical Applications: ELISA Enzyme kinetics Endotoxin assays Cell growth stdies Cytotoxicity Protein assays Reading speed Physical Characteristics 96 wells: 8 seconds Power Volts AC 50/60 Hz Dimensions Weight Reglatory 15.5"W x 16"D x 8.75"H (39.4 x 40.6 x 22.2 cm) 30 lbs (13.6 kg) Specifications are sbject to change. For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. 26 BioTek Readers ELx808

27 ELx800 Absorbance Microplate Reader The ELx800 is a compact, robst microplate reader ideally sited for applications within the clinical and life science research laboratories. Reliable And Robst Design These characteristics are the reason there have been more than 10,000 ELx800s placed in laboratories arond the globe. There simply isn t a more reliable reader with proven performance than the ELx800. Gen5 Software Expands Versatility Under compter control by Gen5 Data Analysis Software, ELx800 applications are expanded to inclde kinetic and well area scanning measrements. Data analysis and reporting/exporting featres in Gen5 are completely cstomizable to sit yor laboratory s reqirements. High Performance, High Vale The ELx800 comes with for filters (ser-selectable wavelengths), with a five filter capacity. In addition to standard 96-well microplate reading, the ELx800 offers 6-, 12-, 24-, 48- and optional 384-well microplate reading, to fit a variety of assay needs. Specifications General Detection method Read method Microplate types Software Onboard software Absorbance Light sorce Wavelength selection Wavelength range Bandpass Dynamic range Resoltion Filter wheel capacity Filters spplied OD accracy OD linearity OD repeatability Absorbance End point, kinetic and area scanning nder compter control 6- to 384-wells 60/72/96-well Terasaki plates (NB option) Gen5 Data Analysis Software 55 ser-programmable protocols Tngsten halogen Filters nm nm (UV option) 10 nm OD OD 5 positions 4 filters (5 with UV option) <1% at 2.0 OD typical <1% at 2.0 OD typical <3% at 3.0 OD typical <0.5% at 2.0 OD typical Reading speed Physical Characteristics 96 wells: 30 seconds Typical Applications: ELISA Protein assays Cytotoxicity Power Volts AC 50/60 Hz Dimensions Weight Reglatory 15 W x 16.5 D x 7 H (38.1 x 41.9 x 17.8 cm) 18.5 lbs (8 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. BioTek Readers ELx

28 Absorbance Reader Comparison Chart Which Absorbance Reader is right for yo? Key Featres Monochromator-Based PowerWave HT Eon Epoch Wavelength range (nm) Microplate types 96 and to to 384 Absorbance range (Abs) Temperatre control to 50 C to 65 C Shaking Linear Orbital and Linear Cvette measrement Cvette adapter Cvette port Take3 or cvette adapter ("C" model) Filter capacity n/a n/a n/a Atomation ready/biostack ("R" model) compatible Gen5 Software inclded Take3 Micro-Volme Plate compatible Fastest read speed: 96 wells (seconds) Typical Performance OD accracy 1% OD 0 to 2 OD: ±1% ±0.010 OD 2 to 3 OD: ±3% ±0.010 OD OD linearity ±1% 0 to 2 OD: ±1% ±0.010 OD 2 to 3 OD: ±3% ±0.010 OD OD repeatability 0.5% ±0.005 OD 0 to 2 OD: ±1% ±0.005 OD 2 to 3 OD: ±3% ±0.005 OD 0 to 2 OD: +1% OD 2 to 2.5 OD: +3% OD 0 to 2 OD: +1% OD 2 to 2.5 OD: +3% OD 0 to 2 OD: +1% OD 2 to 2.5 OD: +3% OD Resoltion OD OD OD 28 BioTek Readers Absorbance Reader Comparison Chart

29 Filter-Based ELx808 IU ELx808 ELx800 ELx800 UV ELx800 NB to 96 6 to 96 6 to to 50 C Linear Linear <1% at 2.5 OD <2% at 3.5 OD <1% at 2.5 OD <2% at 3.5 OD <1% at 2.5 OD <1% at 2.5 OD <1% at 2.0 OD <3% at 3.0 OD <0.5% at 2.5 OD <1.5% at 3.5 OD <0.5% at 2.5 OD <1.5% at 3.5 OD <1% at 2.0 OD <1% at 2.0 OD <1% at 2.0 OD <1% at 2.0 OD <3% at 3.0 OD <1% at 2.0 OD <3% at 3.0 OD <0.5% at 2.0 OD <0.5% at 2.0 OD <0.5% at 2.0 OD OD OD OD OD OD 29

30 Gen5 Data Analysis Software Gen5 Data Analysis Software incorporates over 30 years of experience and ser feedback into otstanding microplate reader software. Gen5 is a niqe combination of power and ease-of-se that drives prodctivity and saves time. Use Gen5 to control BioTek s readers and export data, or as a flly integrated processing tool. Beginner-friendly Software Gen5 is bilt arond logical laboratory workflows to read microplates and prodce/analyze data. In Gen5, yo simply click Read Now and follow the prompts. At the end of the read, answer the qestion: Do yo want to export to Excel? With Gen5 yo don t have to spend hors figring ot how to get things done. Powerfl Fnctionality Gen5 comes with powerfl bilt-in tools sch as 4-P and 5-P crve fits with or withot weighting, parallel-line analysis, advanced kinetic analysis, and mch more. The software has been specifically designed to analyze matrices of data that are difficlt to process in Microsoft Excel spreadsheets. Special attention has been placed on reslt presentation so complex data can be displayed in a clean, colorfl way to facilitate data interpretation. Up-to-date Web-based Sample Files A searchable library of sample files with data is available on the BioTek web site. Existing files are kept p-to-date and new files are added on a reglar basis. Gen5 sers can pload files to share their experiments and sample data with other Gen5 sers. Gen5 Secre: Yo Are In Control Gen5 Secre is for yo if secred data storage, ser grop management or 21 CFR part 11 compliance are a core part of yor reqirements. It incldes extra featres sch as 25 licenses per copy, qality control trending modle with Levey-Jennings charts and atomatic notification on trigger events. All-in-one Soltion Control any BioTek reader, atomate the entire process and prodce pblication-ready reports with one integrated, powerfl software. It doesn t get more efficient than this. High-resoltion 99 x 99 area scan Parallel line analysis and EC 50 determinations 30 BioTek Readers Gen5

31 Specifications General Dose-response EC 50 / IC 50 calclation Compatible readers Validation gidelines Software validation package All crrent BioTek microplate readers Validated per US FDA and ISO 9001/ISO reqirements Available Kinetic analysis Spectral analysis Max slope, mean slope, min/max signal, lag time, Y intercept, onset time, area nder the crve, cstom Min/max signal, peak wavelength, integral, cstom Operating system Minimm reqirements Microsoft Windows 7 32 and 64 bits, Windows Vista and Windows XP (Pro editions) Pentim III 500 MHz, 512 MB RAM, 2 GB hard drive space Qalitative analysis Validation and QC QC trending Mlti-ctoff data analysis Levey-Jennings charts (Gen5 Secre) Plate geometries >80 pre-programmed, ser programmable Pass/fail validation criteria Yes Langages available: Reader Control Simltaneos control Pathlength correction English, Chinese, Rssian, Japanese 2 readers On select readers Z analysis Yes Statistics (mean, stdev, %CV) Yes, atomatically calclated on replicates Exporting Format.xls,.xlsx,.xlsm,.csv,.txt Steps per seqence Unlimited Qick export One click any data / graph on screen Detection modes per seqence Mlti-mode kinetic Area scan resoltion Shaking dration Data Analysis Protocol type Sample types Transformation steps Basic transformations Crve fits Unlimited Up to 3 detection modes per kinetic loop Up to 99 x 99 (9,800 data points per well) on select readers 16-mintes stand-alone step; Unlimited continos shaking in kinetic cycles Standard (plates are independent) Calibrator (1 calibration plate, mltiple sample plates) Mlti-Plate (standard and controls on several plates) Paneled (mltiple assays per plate) Standards, assay controls, sample controls (spikes), blanks, samples Unlimited Blanking, ratio, delta, normalization, cstom Linear, polynomial (degree 2 to 6), 4-P, 5-P, Logit-Log, Point to Point, Spline Weighting 1/Y, 1/Y 2, 1/(Std dev Y 2 ), 1/s 2 Parameter constraint Parallel line analysis Estimate all, Y intercept, fixed parameters Standard, non linear analysis for relative potency calclation PowerExport ASCII export Nmber of exports per file Reporting Nmber of reports per file Report type Ato / cstomizable embedded Microsoft Excel spreadsheet Ato / cstomizable text export Unlimited Unlimited Gen5 Secre 21 CFR Part 11 Compliance System administrator Protected fnctions Time-stamped adit trails Electronic signatres Secre record storage Windows athentication notification Per plate: All data regroped per microplate Per sample: All data regroped per sample Creation and maintenance of ser acconts and ser grops Per ser accont Data and protocol adit trails Data and protocols Gen5 files stored in secre shared-access database Single Sign On (SSO) available Atomatic notification based on trigger event Specifications are sbject to change. 31

32 Take3 Micro-Volme Plate Qickly qantify ltra-low volme samples of DNA, RNA and protein. Measre p to 48 samples with volmes as low as 2 µl withot diltion. Take3 can be sed to measre a standard cvette or patented BioCells for qick 1 cm measrements. Low volme, higher throghpt is available with the Take3 Trio. Compatible with Most BioTek Detection Systems Epoch, Eon and Synergy reader fnctionality can easily reach into the micro-volme range sing the Take3 plate. Measre mltiple 2 µl samples, cvettes or BioCells. Adding the Take3 plate to a BioTek detection system creates an incredibly versatile workstation for a variety of applications. Uniqe Robst Constrction and Easy Maintenance The anodized alminm base constrction, precision crafted slides and hydrophobic sample srfaces make pipetting easy and cleanp effortless. For rotine cleaning of the sample srfaces, a laboratory wipe is all that s needed. If a slide becomes damaged, replacement is easy no need to retrn the Take3 to the factory for repair or calibration. Gen5 Take3 Modle: Atomated DNA, RNA and Protein Qantification It coldn t be easier to get mltiple (p to 48) ncleic acid or protein sample reslts. Gen5 s Take3 modle incldes pre-programmed protocols with immediate reslts otpt inclding spectral scans and prity ratios. There s no need for complicated configration or calclation. Typical Applications: Specifications Micro-volme DNA, RNA and protein qantification Micro-volme florescence measrements in Synergy readers Florescent dye incorporation measrements Spectral scanning in micro-volme, cvette or BioCell Take3 Take3 Trio 2 µl sample capacity Detection limit 2 ng/µl dsdna 2 ng/µl dsdna BioCell capacity 2 2 Cvette capacity 1 Specifications are sbject to change. 32 BioTek Readers Take3

33 Reader Accessories BioTek offers a wide range of accessories to help increase prodctivity, expand yor plate reader s capabilities, and maintain the performance of yor BioTek microplate reader system. See or web site for a complete listing of available accessories. Dal Reagent Dispenser Atomate inject/read assays sch as flash lminescence assays (ATP, lciferase) and florescent ion channel assays on all Synergy readers. Instrment Qalification See the Compliance Section on pages for details abot BioTek s prodct qalification tools and services. Filters and Mirrors Synergy H1 Gas Controller The Gas Controller modle for the Synergy H1 allows fll control over CO 2 and O 2 concentrations to modlate the environment for microplate-based live cell assays. A fll range of standard and cstom filters and dichroic mirrors are available for applications from the low UV to the near infrared. Gen5 Secre Software Upgrade to Gen5 Secre for 21 CFR Part 11 compliance, ser management featres, data encryption and mch more. BioStack Microplate Stacker Atomate rotine processes with this compact stacker. BioStack is also compatible with BioTek s liqid handling instrments. A searchable Accessory database, inclding descriptions and photos, is available on 33

34 34 BioTek Washers Overview

35 Wash BioTek is world renowned for manfactring the most reliable and versatile microplate washers on the market. From basic ELISA to sensitive cell washing to bead washing (inclding Lminex xmap technology), the EL406, 405 Toch, 405 LS and ELx50 are configred with many options to meet a myriad of assay reqirements. Each is eqipped with comprehensive and easy-to-se onboard software for the tmost flexibility in operation. BioTek s Liqid Handling Control Software adds the convenience of assay-specific protocol definition in a Windows environment. The EL406, 405 Toch and 405 LS are compatible with BioTek s BioStack Microplate Stacker for increased throghpt and nattended operation. 35

36 EL406 Microplate Washer Dispenser The EL406 Combination Washer Dispenser is the only instrment on the market offering fast microplate washing together with BioTek s niqe Parallel Dispense technologies for optimized liqid handling processes. Unattended Atomation of ELISAs and Cell-based Assays The EL406 integrates 96-, 384- and 1536-well microplate washing with three dispensers in one compact instrment. Now yo can simply press a btton and walk away, or atomate an entire batch by adding a BioStack Microplate Stacker. A range of EL406 models provides configrability for a lab s specific reqirements. Patented Dal-Action Manifold and Ultrasonic Advantage The EL406 incorporates BioTek s Dal-Action manifold for thorogh yet gentle washing of loosely adherent cell layers, and Ultrasonic Advantage for atomated wash manifold maintenance. Parallel Dispense Technologies The EL406 eliminates the need to choose a dispensing technology by offering both peristaltic and syringe pmps on a single platform. Fast and Efficient Biomagnetic Separation and Vacm Filtration The EL406 atomates fll microplate washing of magnetic microspheres sed in an increasing nmber of mltiplex assays. Developed in conjnction with Lminex xmap technology leaders, BioTek s separation modles incorporate high energy neodymim iron boron magnets for speed and efficiency. An available vacm filtration modle makes the EL406 also well sited for polystyrene beads and filtration-to-waste processes. Typical Applications: ELISA atomation MSD assay atomation High content screening immnocytochemistry Cell-based assays FLIPR Ca 2+ flx Magnetic bead assay atomation Polystyrene bead assay atomation Drg transport assays 36 BioTek Washers EL406

37 Specifications General Assays Separation Microplate types Shaking Soak time Magnet Safety/ convenience/ maintenance Atomation Onboard software Software Washing Manifold types Washing speed Flid delivery Vacm filtration Volme range Bffer selection Flow rates Wash cycles Dispense accracy Dispense precision Residal volme Sterilization ELISA Cell-based assays (model dependent) Magnetic bead, polystyrene bead (optional) Mltiplex assays Bead-based ELISA Filtration-to-waste processes (optional) Biomagnetic separation, vacm filtration (optional) 96-, 384- and 1536-wells (model dependent) Low profile and standard height Solid and filter bottom (optional) Programmable in mintes and seconds, p to 60 mintes Intensities - slow, medim, fast or variable Programmable in mintes and seconds, p to 60 mintes High strength 96- and 384-well designs Flat Flat-bottom well - beads plled to band across well bottom Rond-bottom well - beads plled to btton at well bottom Ring - beads plled to 4-zone ring at well bottom Waste and vacm sensing, flid and flow detection Aerosol cover Adjstment tility for plate positioning Overflow protection Pre-programmed maintenance rotines Compatible with BioStack (exclding filter bottom plates) and 3rd party atomation Create, edit or rn mltiple protocols Liqid Handling Control, for PC protocol programming and exection (optional) 96-well washing: 96-tbe (8x12) manifold stainless steel tbes 96-/384-well washing: Dal-Action 96-tbe (8 x 12) manifold stainless steel tbes 384-well washing: Dal-Action 192-tbe (16 x 12) manifold stainless steel tbes 1536-well washing: Two 32-tbe (1 x 32) dispense manifolds - sapphire jeweled 316 stainless steel or 316 stainless steel tbes 128-tbe (4 x 32) aspiration manifold Solid bottom plates: 1 asp./disp. cycle: 300 µl/well, 96 wells, 96-tbe manifold: 13 seconds 100 µl/well, 384 wells, 192-tbe manifold: 17 seconds 10 µl/well, 1536 wells, two 32-tbe manifolds: 36 seconds 96-/384-well washing: One positive displacement pmp 1536-well washing: Two positive displacement syringe drives Selectable vacm levels: 0 to -380 mmhg (final at 30 seconds) Vacm filtration time range: seconds 3-3,000 µl/well (model dependent) Selectable in 1 µl increments Atomatic switching for p to 4 wash bffers (model dependent) 1-11, inclding low flow cell wash rates (model dependent) ±3% typical 3% CV (model dependent) 2 µl/well Chemical Ultrasonic cleaning Spply bottle Bilt-in ltrasonic bath to remove protein and salt crystal bildp 4 L or 10 L (optional) Dispensing - Peristaltic Pmp Manifold types 8-tip cassette (1 x 8) - sapphire jeweled 316 stainless steel, 316 stainless steel or polypropylene plastic tips Dispensing speed Volme range Flid delivery Flow rates Cassette size Sterilization 10 µl/well, 96 wells, 8-tip cassette: 8 seconds 5 µl/well, 384 wells, 8-tip cassette: 12 seconds 1 µl/well, 1536 wells, 8-tip cassette: 27 seconds 1-3,000 µl/well Selectable in 1 µl increments One positive displacement peristaltic pmp Low, medim or high 1 µl: Recommended Volme Range: 1-50 µl Dispense Accracy: ±5.0% typical at 1 µl Dispense Precision: 5.0% CV typical at 1 µl Minimm Prime Volme (30 tbing length): 1.20 ml 5 µl: Recommended Volme Range: 5-2,500 µl Dispense Accracy: ±2.0% typical at 5 µl Dispense Precision: 2.5% CV typical at 5 µl Minimm Prime Volme (30 tbing length): 4.23 ml 10 µl: Recommended Volme Range: µl Dispense Accracy: ±2.0% typical at 10 µl Dispense Precision: 2.0% CV typical at 10 µl Minimm Prime Volme (30 tbing length): 7.36 ml Atoclave, Chemical Dispensing - Syringe Pmp Manifold types Dispensing speed Volme range Flid delivery Flow rates 1-5 Dispense accracy Dispense precision Reagent selection Spply bottle Sterilization Physical Characteristics 96-well dispensing: One 16-tbe (2 x 8) manifold stainless steel tbes 96-/384-well dispensing: Two 16-tbe (1 x 16) manifolds stainless steel tbes 1536-well dispensing: Two 32-tbe (1 x 32) manifolds - sapphire jeweled 316 stainless steel or 316 stainless steel tbes 10 µl /well, 96 wells, 1 x 16 or 1 x 8 tbes: 9 seconds 5 µl /well, 384 wells, 1 x 16 tbes: 11 seconds 3 µl /well 1536 wells, 2 x 32 tbes: 14 seconds 3-3,000 µl/well Selectable in 1 µl increments Minimm Prime Volme: 12 ml Two positive displacement syringe drives ±1 µl typical at 5 µl ±1 µl typical at 20 µl ±1% typical at 100 µl 5% CV typical at 5 µl 2.5% CV typical at 20 µl 1% CV typical at 100 µl Atomatic switching for p to 4 reagents (optional) 1 L or 2 L (model dependent) Atoclave (model dependent), Chemical Power Volts AC 50/60 Hz Dimensions Weight Reglatory 16.5 W x 18 D x 12.5 H (42 x 46 x 32 cm) 32 lbs (14.5 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 37

38 405 Toch Microplate Washer BioTek s 405 Toch Microplate Washer takes plate washing to the next level with an enhanced ser interface, increased convenience and flexible assay applications. Indstry Leading, User Pleasing The 405 Toch Microplate Washer incorporates all the featres and fnctionality of the prior ELx405 models, and improves accessibility throgh its toch screen and extensive onboard software. 96- and 384-well microplate based wash procedres are only two toches away with the easy-to-se interface. Additionally, two USB flash drives provide convenient file transfer, storage and operation. A context sensitive Help system and several instrctional videos are also inclded. The Standard for Atomation The new 405 Microplate Washer makes qick work of any washing assay, and is especially well sited for integration into atomated systems, where the wash process is controlled remotely. The 405 range incldes models for washing 96- and 384-well microplates and a vale added featre set in the 405 LS models. Cell and Bead Assays The 405 is available in varios models for optimized performance with the most sensitive and rigoros assay reqirements. When the protocol calls for washing loosely adherent cells, the Select model is fine-tned with angled dispense tbes, extra low flow rates and niqe X, Y and Z positioning. Magnetic and polystyrene bead washing are effectively accomplished with the 405. Bffer Switching and Atomated Ultrasonic Cleaning BioTek s patent pending Ultrasonic Advantage, an available option for all 405 Microplate Washers, incorporates powerfl ltrasonic cleaning, making the 405 selfmaintaining. Up to 4 bffers may be connected sing the optional internal bffer switch valve system to save valable bench space and improve system sability. Applications in Deep Well Washing The ELx405 Select Deep Well washes 96- and 384-well plates p to 50 mm tall, and is also compatible with standard height plates withot any hardware or software changes. This versatile system is optimal for labs working in deep well blocks and standard plates. Typical Applications: ELISAs MSD assays HCS immne cytochemistry FLIPR Ca 2+ flx Cell-based assays Magnetic and polystyrene bead assays Gene expression assays Cytokine assays ELISPOT assays Plasmid DNA prification Serm/plasma sample preparation Cell signaling phospho flow setp for flow cytometry 38 BioTek Washers 405 Toch

39 Specifications General Assays Separation Microplate types Shaking Soak time Magnet Safety/ convenience/ maintenance Atomation Onboard software/gui Software Interface Operating temperatre Hmidity Electrical Washing Manifold types Washing speed ELISA Cell-based assays (model dependent) Magnetic bead, polystyrene bead (model dependent) Mltiplex assays Bead-based ELISA Filtration-to-waste processes (optional) Biomagnetic separation, vacm filtration 96- and 384-wells (model dependent) Low profile and standard height Deep well p to 50 mm (model dependent) Solid and filter bottom (optional) Filter pore sizes 0.45 µm to 1.2 µm Programmable in mintes and seconds, p to 60 mintes Intensities slow, medim, fast or variable Programmable in mintes and seconds, p to 60 mintes High strength 96- and 384-well designs Choice of two designs, cstom for BioTek microplate washers: Flat Flat-bottom well - beads plled to band across well bottom Rond-bottom well - beads plled to btton at well bottom Ring - beads plled to 4-zone ring at well bottom Waste and vacm sensing, flid and flow detection Aerosol cover Adjstment tility for plate positioning Overflow protection Pre-programmed maintenance rotines BioStack Microplate Stacker p to 50 ANSI/SBS standard height plates (optional) Except filter bottom plates 5.7 High resoltion backlit LED color toch screen Intel Atom processor rnning Windows CE Predefined sample wash protocols ELISA, cell wash, biomagnetic separation and vacm filtration Create or edit mltiple cstom protocols Rn protocols created onboard, downloaded from LHC Software or transferred on USB flash drive Liqid Handling Control (LHC), for PC wash protocol programming and exection (optional) For PC control and BioStack integration 1 USB port, 1 9-pin RS232 port For file transfer 2 USB Flash Memory ports 15 C to 30 C (59 F to 86 F) 10% to 85%, non-condensing Volts AC 50/60 Hz, 8.0 A 96-well washing: 96-tbe (8 x 12) manifold 96-/384-well washing: 96-tbe (8 x 12) Dal-Action manifold 384-well washing: 192-tbe (16 x 12) Dal-Action manifold Solid bottom plates: 96-well: 3 aspirate / dispense cycles, 300 µl/well, 96-tbe manifold, final aspirate: 30 seconds 384-well: 3 aspirate / dispense cycles, 100 µl/well, 96-tbe manifold, final aspirate: 80 seconds 1 aspirate / dispense cycle, 400 µl/well, 192-tbe manifold, final aspirate: 20 seconds Filter bottom plates: Variable, based on wash parameters Flid delivery Vacm filtration Volme range Bffer selection Flow rates Wash cycles 1 to 250 Dispense precision Residal volme Sterilization Ultrasonic cleaning Spply bottle Physical Characteristics Dimensions Weight Reglatory One internal positive displacement pmp Selectable vacm levels (final at 30 seconds): Lowest -38 mmhg Low -113 mmhg Medim -200 mmhg High -390 mmhg Highest -506 mmhg Vacm filtration time range: 5 to 999 seconds 25 to 3,000 µl/well Selectable in 1 µl increments Internal atomatic switching for p to 4 wash bffers (optional) 1-11, inclding low flow cell wash rates (model dependent) 96-well washing 96-tbe (8 x 12) manifold: 3% CV: 300 µl/well (deionized water with 0.1% Tween 20 and FD&C #1 ble dye), rate 6, absorbance read at 630 nm and 450 nm reference 384-well washing 192-tbe (16 x 12) manifold: 4% CV: 80 µl/well (deionized water with 0.1% Tween 20 and FD&C #1 ble dye), rate 7, absorbance read at 630 nm and 450 nm reference Solid bottom plates: 96-well washing 96-tbe (8 x 12) manifold: 2 µl/well: 3-cycle wash, 300 µl/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing 384-well washing 192-tbe (16 x 12) manifold: 2 µl/well: 3-cycle wash, 100 µl/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing Filter bottom plates: 96-well: Average increase weight of plate 1.2 g after dispensing 300 μl to 0.45 μm plate, filtration 30 seconds, low vacm, blotted 384-well: Average increase weight of plate 4.0 g after dispensing 80 μl to 1.2 μm plate, filtration 10 seconds, low vacm, blotted Chemical: 0.5% sodim hypochlorite, 70% isopropyl alcohol or 70% ethanol soltion Bilt-in ltrasonic bath for protein and salt crystal bildp Programmable in hors and mintes, p to 60 mintes (model dependent) 4 L or 10 L (optional) Depth: 14 W x 17 D x 10 H (35.6 x 43.2 x 25.4 cm) With internal bffer switching 36 lbs (16.5 kg) Withot 30 lbs (13.5 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 39

40 ELx50 Microplate Strip Washer The ELx50 Microplate Strip Washer s compact footprint conceals a powerhose of washing capabilities nsrpassed in its class. Exceptional dispense precision and evacation efficiency can be tilized for both 96- and 384-well strip and plate washing. Mltiple Washers in One The ELx50 is a 3-in-1 soltion for 96-well plate formats atomating the wash steps of ELISAs, magnetic bead assays and polystyrene bead assays. The ELx50 can also be eqipped with BioTek s patented Dal-Action manifold allowing independent control of dispense and aspiration manifolds in both 96- and 384-well formats. As a welcome pgrade from manal processing, the ELx50 provides an all-inclsive wash soltion offering consistent performance and nattended operation. Syringe Drive Flid Delivery As a self-contained and programmable washer, the ELx50 allows for complete control of precise flidic delivery from the gentle dripping of a simple sqeeze bottle to the fll force of pressre delivery systems. Comprehensive onboard software makes creating protocols qick and intitive. Atomated Liqid Level Sensing Liqid Level Alert allows the convenience of continos monitoring for both spply and waste bottles. At the beginning and end of a wash protocol, the liqid level is verified to ensre an adeqate bffer remains to complete a wash. Sfficient storage capacity in the waste bottle is also verified. Typical Applications: ELISAs Cell-based assays Magnetic bead assays Polystyrene bead assays ELISPOT assays Mltiplex assays 40 BioTek Washers ELx50

41 Specifications General Assays Separation Microplate types Shaking Soak time Magnet Safety/ convenience/ maintenance Onboard software Washing Manifold types Washing speed ELISA Cell-based assays Magnetic bead, polystyrene bead (model dependent) Mltiplex assays Bead-based ELISA Filtration-to-waste processes (model dependent) Protein arrays Biomagnetic separation, vacm filtration (model dependent) 96- and 384-wells (model dependent) Low profile and standard height Solid and filter bottom (model dependent) Filter pore sizes 0.45 µm to 1.2 µm Programmable in mintes and seconds p to 60 mintes Intensities - slow, medim, fast or variable Programmable in mintes and seconds p to 60 mintes High strength 96-well format Flat Flat-bottom well - beads plled to band across well bottom Rond-bottom well - beads plled to btton at well bottom Ring - beads plled to 4-zone ring at well bottom Waste sensing, flid detection (optional) Aerosol cover Overflow protection Pre-programmed maintenance rotines Create, edit or rn mltiple protocols 96-well washing: 8-tbe (1x8) manifold 12-tbe (1x12) manifold 96-/384-well washing: Dal-Action 16-tbe (1x16) manifold Solid bottom plates: 3 asp./disp. cycles: >300 µl/well, 96 wells, 8-tbe manifold, final aspirate: 130 seconds Filter bottom plates: Variable, based on wash parameters Flid delivery Vacm filtration Volme range Bffer selection Flow rates 1-9 Wash cycles 1-10 Dispense precision Residal volme Sterilization Spply bottle One positive displacement syringe drive Selectable vacm levels (final at 30 seconds): 0.45 µm 96-well plates: Low: -91 mmhg Medim: -150 mmhg High: -313 mmhg 1.2 µm 96-well plates: Low: -95 mmhg Medim: -155 mmhg High: -299 mmhg Vacm filtration time range: seconds 25-3,000 µl/well (model dependent) Selectable in 1 µl increments Atomatic switching for p to 3 wash bffers (model dependent) 3% CV Solid bottom plates: 2 µl/well Filter bottom plates: Average increase weight of plate 1.2 g after dispensing 300 µl to 0.45 µm plate, filtration 30 seconds, low vacm, blotted Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodim hypochlorite soltion 2 L Physical Characteristics Power Volts AC 50/60 Hz Dimensions Weight Reglatory 14 W x 16 D x 6.5 H (35.6 x 40.6 x 16.5 cm) 22 lbs (9.8 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 41

42 Washer Comparison Chart Which Washer is right for yo? EL406 Microplate Washer Dispenser 405 Toch Microplate Washer Key Featres ELISA Cell-based assays Magnetic bead assays Polystyrene bead assays Filtration-to-waste processes Toch screen ser interface USB ports for protocol transfer Performance Specifications Washing speed - 3 aspirate/dispense cycles, 96- well solid bottom plate, 300 µl/well 30 sec 30 sec Dispense precision 3% CV 3% CV Residal volme - solid bottom plate 2 µl/well 2 μl/well Vacm filtration µm 96-well plate 0 mmhg to -380 mmhg -38 mmhg to -506 mmhg General Specifications Microplate types 96, 384 and and 384 Low profile and standard height Solid and filter bottom Deep well Manifold 96-well washing 96-tbe (8x12) 96-tbe (8x12) 96-/384-well washing Dal-Action 96-tbe (8x12) Dal-Action 96-tbe (8x12) 384-well washing Dal-Action 192-tbe (16x12) Dal-Action 192-tbe (16x12) 1536-well washing Dispense: Two 32-tbe (1x32); Aspiration: 128-tbe (4x32) Atomation ready/biostack compatible Atomatic bffer switching Up to 4 Up to 4 Flow rates 1-11, inclding low flow cell wash 1-11, inclding low flow cell wash Volme range µl/well μl/well Microplate shaking Flid and waste detection Flow and vacm detection Overflow protection Pre-programmed maintenance rotines Bilt-in ltrasonic cleaner Aerosol cover Onboard software inclded Liqid Handling Control Software compatible 42 BioTek Washers Washer Comparison Chart

43 405 LS Microplate Washer ELx405 Select Deep Well Washer ELx50 Microplate Strip Washer 30 sec 30 sec 130 sec 3% CV 3% CV 3% CV 2 μl/well 2 μl/well 2 µl/well -38 mmhg to -506 mmhg -95 mmhg to -299 mmhg 96 and and and tbe (8x12) 8-tbe (1x8) or 12-tbe (1x12) Dal-Action 96-tbe (8x12) Dal-Action 96-tbe (8x12) Dal-Action 16-tbe (1x16) Dal-Action 192-tbe (16x12) Up to 4 Up to 4 Up to , inclding low flow cell wash 1-11, inclding low flow cell wash μl/well μl/well µl/well 43

44 Liqid Handling Control Secre Software Liqid Handling Control (LHC ) Secre Software offers fll control of the EL406 Washer Dispenser, 405 Toch and 405 LS Washer and MltiFlo Microplate Dispenser with StepWise protocol creation that even novice sers will find easy to se. 21 CFR Part 11 Compliance To meet the demands of the GxP laboratory, LHC Secre offers featres to ensre compliance to 21 CFR Part 11. Flexible mlti-ser permission levels and electronic protocol and system adit trail signing are all available whenever additional secrity is reqired. Dal Mode Control LHC Software is a powerfl yet flexible interface for se with BioTek s washers and dispensers. Any programming seqence possible onboard the liqid handler may be dplicated from within LHC. Wash and dispense protocols can be created and rn from the PC or downloaded to the instrment s keypad. This is particlarly sefl when the instrment is installed in a biological safety cabinet. Protocols can be locked to avoid nintentional keypad modifications. Atomatic Protocol Validation LHC Software allows a virtally nlimited nmber of methods to be linked together for the most complex liqid handling rotines. Protocols are atomatically validated to ensre feasibility and compatibility with the selected instrment. Robotic integration to commercially available systems is also seamless with Liqid Handling Control. Mlti-ser permission levels 44 BioTek Washers Liqid Handling Control Secre Software

45 Washer Accessories BioTek offers a wide range of accessories to help increase prodctivity, expand yor plate washer s capabilities, and maintain the performance of yor BioTek microplate washer system. See or web site for a complete listing of available accessories. Biomagnetic Separation Dispense/Waste Systems A dispense/waste system is reqired on all 405 Toch, 405 LS and EL406 models. Many selections are available based on throghpt, bottle size and vacm pmp reqirements. A range of high-strength 96- and 384-well format magnets is available in flat and 4-zone ring designs. Peristaltic Pmp Dispenser Cassettes Fast and Efficient Vacm Filtration An available vacm filtration modle atomates the washing of 96-well filter bottom plates. Vacm is adjsted via a range of settings for optimal performance with varios filter pore sizes. A wide selection of EL406 peristaltic pmp cassettes is available with choices in volme ranges, tip materials and bore sizes. Instrment Qalification See the Compliance Section on pages for details abot BioTek s prodct qalification tools and services. BioStack Microplate Stacker Atomate rotine processes with this compact stacker. BioStack is also compatible with BioTek s microplate readers, dispensers and pipetting systems. A searchable Accessory database, inclding descriptions and photos, is available on 45

46 46 BioTek Dispensers Overview

47 Dispense BioTek offers two versatile microplate dispenser platforms, the MltiFlo and the MicroFill. While the MltiFlo featres Parallel Dispense technologies and mltiple reagents, the MicroFill serves as a robst and longstanding soltion for single reagent, syringe-based dispensing. Both are compatible with the BioStack Microplate Stacker for increased throghpt and walk-away atomation. Liqid Handling Control (LHC ) Software expands the MltiFlo s keypad capabilities with a graphical PC interface for advanced control. 47

48 MltiFlo Microplate Dispenser BioTek s MltiFlo Microplate Dispenser replaces p to for independent dispensers in one compact instrment, redcing costs, saving time and simplifying processes. Flly Modlar and Upgradable The MltiFlo is a 1-to-4 reagent, all-in-one dispenser designed on an pgradable platform. Users can prchase one channel now and pgrade to more later when the laboratory s needs change. With its compact footprint and base height of less than 8 inches, the MltiFlo comfortably fits on any lab bench or robotic system. Parallel Dispense Technologies Offering BioTek s niqe combination of peristaltic and microprocessor controlled syringe pmp dispensing, the MltiFlo enables sers to choose which is best for a specific reagent. While peri pmps offer low prime volmes and backflsh capabilities, BioTek s syringe drives are program-and-forget soltions that never reqire recalibration. Up to two of each dispense type is available on a single MltiFlo. Broad Dispense and Microplate Ranges A wide array of plate type settings accommodate 6- to 1536-well formats and p to 50 mm in height. Volmes from 1 μl to 3 ml are accrately and precisely dispensed. The MltiFlo is easily integrated with a BioStack Microplate Stacker, where p to well plates can be atomated with walk-away confidence. Typical Applications: Primary and secondary screening assays Compond storage Genomics and proteomics research Cell-based assays ELISAs 48 BioTek Dispensers MltiFlo

49 Specifications General Nmber of reagents Shaking Soak time Safety/ convenience/ maintenance Atomation Onboard software Software 1-4 (model dependent) Dispensing - Peristaltic Pmp Microplate types Manifold types Dispensing speed Volme range Flid delivery Flow rates Cassette size Recommended replacement interval Programmable in mintes and seconds, p to 60 mintes Intensities slow, medim, fast or variable Programmable in mintes and seconds, p to 60 mintes Adjstment tility for plate positioning Pre-programmed maintenance rotines Compatible with BioStack and 3rd party atomation Create, edit or rn mltiple protocols Liqid Handling Control, for PC protocol programming and exection (optional) 6- to 1536-wells in low profile, standard height and deep well formats (manifold dependent) PCR plates Microtbes Maximm height: 47 or 50 mm (carrier dependent) 8-tip cassette (1 x 8) - sapphire jeweled 316 stainless steel, 316 stainless steel, or polypropylene plastic tips 10 µl/well, 96 wells, 8-tip cassette: 3 seconds 5 µl/well, 384 wells, 8-tip cassette: 7 seconds 1 µl/well, 1536 wells, 8-tip cassette: 21 seconds 1-3,000 µl/well Selectable in 1 µl increments One or two positive displacement peristaltic pmps Low, medim or high Specifications are sbject to change. 1 µl: Recommended Volme Range: 1-50 µl Dispense Accracy: ±5% typical at 1 µl Dispense Precision: 5% CV typical at 1 µl Minimm Prime Volme: ml 5 µl: Recommended Volme Range: 5-2,500 µl Dispense Accracy: ±2% typical at 5 µl Dispense Precision: 2.5% CV typical at 5 µl Minimm Prime Volme: ml 10 µl: Recommended Volme Range: 10-3,000 µl Dispense Accracy: ±2% typical at 10 µl Dispense Precision: 2% CV typical at 10 µl Minimm Prime Volme: ml 1 µl Cassette: 1, well microplates at 5 µl/well 5 µl Cassette: 1, well microplates at 50 µl/well 10 µl Cassette: 1, well microplates at 100 µl/well Sterilization Dispensing - Syringe Pmp Microplate types Manifold types Dispensing speed Volme range Flid delivery Flow rates 1-5 Dispense accracy Dispense precision Minimm prime volme Spply bottle volme Sterilization Atoclave: Up to 50 cycles at temperatres and pressres of 121 C and 1 bar (750 mmhg) Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodim hypochlorite soltion 96-, 384- and 1536-wells in low profile, standard height and deep well formats (manifold dependent) PCR plates Microtbes Maximm height: 47 or 50 mm (carrier dependent) 96-/384-well dispensing: One 16-tbe (2 x 8) manifold 316 stainless steel tbes 96-/384-well dispensing: Two 16-tbe (1 x 16) manifolds 316 stainless steel tbes 1536-well dispensing: Two 32-tbe (1 x 32) manifolds sapphire jeweled 316 stainless steel SB or 316 stainless steel LB tbes 20 µl/well, 96 wells, 1 x16: 5 seconds 20 µl/well, 384 wells, 1 x16: 14 seconds 3 µl/well, 1536 wells, 1 x 32: 14 seconds 3 µl/well, 1536 wells, 2 x 32: 7 seconds 3-3,000 µl/well (manifold dependent) Selectable in 1 µl increments Two positive displacement syringe drives ±1 µl typical at 5 µl ±1 µl typical at 20 µl ±1% typical at 100 µl 5% CV typical at 5 µl 2.5% CV typical at 20 µl 1% CV typical at 100 µl 12 ml Physical Characteristics 1 L or 2 L (model dependent) Atoclave (model dependent): Up to 50 cycles at temperatres and pressres of 121 C and 1 bar (750 mmhg) Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodim hypochlorite soltion Power Volts AC 50/60 Hz Dimensions Weight Reglatory Base instrment: 16.1 W x 10.6 D x 7.6 H (40.9 x 26.9 x 19.3 cm) Base instrment: 14.3 lbs (6.5 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. 49

50 MicroFill Microplate Dispenser With its microprocessorcontrolled syringe drive technology, the MicroFill Microplate Dispenser provides otstanding accracy and precision while dispensing into 24-, 96- and 384-well plates. Low Maintenance Design The MicroFill is an economical, compact and reliable alternative to conventional microplate dispensers. Its microprocessorcontrolled syringe pmp provides optimal dispense performance withot timeconsming recalibration, cassette replacement and maintenance. Syringes are ideal for higher volme filling, with noteworthy speed improvements compared to other dispense technologies. Garanteed Sterility The entire flid path is atoclavable for applications reqiring sterility. The MicroFill s pmp, tbing, dispense manifold and spply bottle are qickly changed for no reagent carryover. User-controlled dispense flow rates allow low- to high-velocity dispensing for both biochemical and cell-based assays. Lowprofile, standard and deep well microplates are all accommodated with a broad volme range from 5 µl to 6 ml. Unattended Operation For increased throghpt, the MicroFill can be integrated with BioTek s BioStack Microplate Stacker or interfaced to third party atomated systems with its available interface software. MicroFill drivers are available from most of today s leading system providers. Typical Applications: Primary and secondary screening assays Compond storage Genomics and proteomics research Cell-based assays ELISAs 50 BioTek Dispensers MicroFill

51 Specifications General Nmber of reagents 1 Microplate types Soak time Safety/convenience/ maintenance Atomation Onboard software 24-, 96- and 384-wells in low profile, standard and deep well formats PCR plates Microtbes Programmable in mintes and seconds, p to 60 mintes Pre-programmed maintenance rotines Compatible with BioStack and 3rd party atomation Create, edit or rn mltiple protocols Software Dispensing - Syringe Pmp Dispensing speed Interface software (optional) for robotic system integration 10 µl/well, 96 wells, 1x16: 4 sec 5 µl/well, 384 wells, 1x16: 7 sec Manifold type Volme range Flid delivery 24-well dispensing: One 8-tbe (1 x 8) manifold 316 stainless steel tbes 96-well dispensing: One 8-tbe (1 x 8) manifold 316 stainless steel tbes 96-/384-well dispensing: One 16-tbe (1 x 16) manifold 316 stainless steel tbes 5-6,000 µl/well (manifold dependent) One positive displacement syringe drive Flow rates 1-5 Dispense accracy ±1 µl typical at 5 µl ±1 µl typical at 20 µl ±1% typical at 100 µl Dispense precision 5% CV typical at 5 µl 2.5% CV typical at 20 µl 1% CV typical at 100 µl Minimm prime volme Spply bottle 10 ml 1 L Sterilization Physical Characteristics Atoclave: Up to 50 cycles at temperatres and pressres of 121 C and 1 bar (750 mmhg) Chemical: 70% ethyl or isopropyl alcohol and 0.5% sodim hypochlorite soltion Power Volts AC 50/60 Hz Dimensions Weight Reglatory 15 W x 18 D x 7 H (38 x 46 x 18 cm) 20 lbs (8.9 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 51

52 Dispenser Comparison Chart Which Dispenser is right for yo? EL406 Microplate Washer Dispenser Key Featres ELISA Cell-based assays Nmber of reagents 1 to 3 Dispense technology Peristaltic and/or Syringe Flly modlar and pgradable Performance Specifications Dispensing speed Peristaltic pmp (8-tip, 1x8) 96-well, 10 µl/well; 384-well, 5 µl/well 8 sec; 12 sec Syringe pmp (16-tbe, 1x16) 96-well, 10 µl/well; 384-well, 5 µl/well 9 sec; 11 sec Dispense accracy - typical at 5 µl Peristaltic pmp ±2% Syringe pmp ±1 µl Dispense precision - typical at 5 µl Peristaltic pmp 2.5% CV Syringe pmp 5% CV General Specifications Microplate types 96, 384 and 1536 Low profile and standard height Deep well Strips and fll plates Cassette/manifold Peristaltic pmp 8-tip (1x8) Syringe pmp 24-well dispensing Syringe pmp 96-well dispensing 8-tbe (1x8), 16-tbe (2x8) Syringe pmp 96-/384-well dispensing 16-tbe (2x8), 16-tbe (1x16) Syringe pmp 1536-well dispensing 32-tbe (1x32) Atomation ready/biostack compatible Flow rates Peristaltic pmp: low, medim, high Syringe pmp: 1-5 Volme range 1-3,000 µl/well Microplate shaking Atoclavable flid path Onboard software inclded Liqid Handling Control Software compatible 52 BioTek Dispensers Dispenser Comparison Chart

53 MltiFlo Microplate Dispenser MicroFill Microplate Dispenser 1 to 4 1 Peristaltic and/or Syringe Syringe 3 sec; 7 sec 4 sec; 6 sec 4 sec; 7 sec ±2% ±1 µl ±1 µl 2.5% CV 5% CV 5% CV 6 to , 96 and tip (1x8) 8-tbe (1x8) 8-tbe (1x8) 16-tbe (2x8), 16-tbe (1x16) 16-tbe (1x16) 32-tbe (1x32) 1-3,000 µl/well 5-6,000 µl/well 53

54 Liqid Handling Control Software Liqid Handling Control (LHC ) Software allows MltiFlo Dispenser, EL406 Washer Dispenser and 405 Toch and 405 LS Washer sers the convenience of programming important assay-specific protocol reqirements in a Windows environment. Expanded Versatility LHC Software is a powerfl yet flexible interface for se with BioTek s microplate dispensers and washers. Any programming seqence possible onboard the liqid handler may be dplicated from the compter with LHC Software. LHC also allows a virtally nlimited nmber of methods to be linked together for the most complex liqid handling rotines. From a washer s first prime rotine, mltiple microplate processes over time, ltrasonic cleaning to dissolve protein or salt crystal bild-p to a final system rinse, LHC Software enables nattended operation. Cstom Maintenance Reminders To facilitate maintenance and keep a washer or dispenser in peak condition, factory recommended maintenance procedre reminders can be preset and cstomized appropriately for a bsy laboratory s sage and throghpt reqirements. LHC also spports BioStack Microplate Stacker integrations. Safe Record Keeping Protocol parameters may be qickly printed for safe record keeping. Alternatively, onboard instrment protocols may be ploaded and backed p on a laboratory s network. Satellite research labs working on joint projects can be certain their wash parameters are identical for experimental integrity. Intitive StepWise protocol creation for ltimate flexibility 54 BioTek Dispensers Liqid Handling Control Software

55 Dispenser Accessories BioTek offers a wide range of accessories to help increase prodctivity, expand yor plate dispenser s capabilities, and maintain the performance of yor BioTek microplate dispensing system. See or web site for a complete listing of available accessories. Peristaltic Pmp Dispenser Cassettes A wide selection of MltiFlo peristaltic pmp cassettes is available with choices in volme ranges, tip materials and bore sizes. External Dispenser Modles Dispense p to three additional reagents with these optional MltiFlo modles. Syringe Pmp Dispenser Manifolds A range of MltiFlo and MicroFill dispense manifolds are available for varios microplate types and reagent characteristics. BioStack Microplate Stacker Atomate rotine processes with this compact stacker. Instrment Qalification See the Compliance Section on pages for details abot BioTek s prodct qalification tools and services. 3-Instrment Rack For third party robotic system integration, a rack is available for spporting p to three dispensers or other BioTek instrmentation. A searchable Accessory database, inclding descriptions and photos, is available on 55

56 56 BioTek Pipetting Overview

57 Pipette The Precision Microplate Pipetting System represents a niqe range of compact and affordable liqid handlers for yor laboratory s specific pipetting needs. Single, 8- and 12-channel transfer tools are available, along with blk reagent dispensers, to meet varied reqirements in a wide range of sample tbes, microplates and reservoirs. 57

58 Precision Microplate Pipetting Systems The Precision is an innovative soltion for atomated liqid handling. With its ability to perform virtally any rotine liqid transfer, Precision replaces tedios manal pipetting. Atomate Manal Pipetting The Precision can be cstomized with a range of options perfect for medim throghpt labs looking to atomate their everyday pipetting with walk-away confidence. BioTek s proprietary pipette technology and niqe tip sealing allow most standard tips to be sed for transfers in common sample tbes and 6- to 384-well microplate formats. Open Deck Layot and Flexible Software A ser-configrable, mltistation deck allows for flexible experimental design; microplates, tips and other labware may be placed in nearly any location for optimal efficiency. Available Precision Power Software offers complete Precision control with intitive protocol creation, expanding the instrment s dynamic capabilities with a graphical program simlator and sample ID tracking. Space Saving, Compact Footprint Its small footprint and wellorganized design makes the Precision ideally sited for installation inside standard size biological safety cabinets and chemical fme hoods. The Precision XS model delivers otstanding liqid handling performance with for liqid transfer tools on a single platform. All for may be intermixed throghot a flly atomated protocol single and mltichannel pipetting along with single- and mlti-channel blk reagent dispensing. Typical Applications: Sample transfers from tbe to microplate Serial diltions Mixing Plate replication - mother/daghter transfers Reagent addition Hit picking ELISA atomation Secondary screening assays Compond profiling Cell-based assays 58 BioTek Pipetting Precision

59 Specifications General Microplate types Atomation Platform Software Pipetting Manifold types 6- to 384-wells, any test tbe 100 mm Compatible with BioStack and 3rd party atomation 6 stations Precision Power, for PC control programming and exection (inclded) 1x8, single-channel Pipetting speed 1x8: 100 µl/well, 96 wells, tip change: 3 min Single-channel: 100 µl/well, 96 wells, tip change: 22 min Volme range 1x8: µl Single-channel: µl Flid delivery Air displacement syringe drives Dispense accracy ±1% typical at 100 µl Dispense precision 1.5% CV typical at 100 µl Pipette tips Dispensing - Syringe pmp Manifold types Dispensing speed Volme range 1x8: 10 µl - 10 ml Single-channel: 5 µl - 10 ml Flid delivery BioTek and other commercially available tips 96-/384-well dispensing: One 8-tbe (1x8) manifold stainless steel tbes 6- to 384-well dispensing: One single-channel probe 100 µl well, 96 wells, 1x8: 14 seconds 100 µl well, 96 wells, single-channel: 4 mintes One positive displacement syringe drive Dispense accracy ±1% typical at 100 µl Dispense precision <1.5% CV typical at 100 µl Spply bottle volme 1x8: 2 L Single-channel: 125 ml Physical Characteristics Power Volts AC 50/60 Hz Dimensions Precision XS Instrment only: 25 W x 16 D x 20 H (640 x 410 x 510 mm ) Instrment with optional cabinet: 30 W x 20 D x 22 H (760 x 510 x 560 mm) General Microplate types Atomation 96- and 384-wells Platform 1x8: 6 stations 1x12: 4 stations Onboard software Software Pipetting Manifold types Compatible with BioStack (exclding 1x12 pipetting) and 3rd party atomation Create, edit or rn mltiple protocols Precision Power, for PC control programming and exection (optional) 1x8, 1x12 (model dependent) Pipetting speed 1x8: 100 µl/well, 96 wells, tip change: 3 min Volme range µl Flid delivery Air displacement syringe drives Dispense accracy ±1% typical at 100 µl Dispense precision 1.5% CV typical at 100 µl Pipette tips Dispensing - Syringe pmp Manifold types Dispensing speed Volme range Flid delivery BioTek and other commercially available tips One 8-tbe (1x8) and/or 12-tbe (1x12) manifold stainless steel tbes 100 µl/well, 96 wells, 1x8: 14 seconds 10 µl - 10 ml One positive displacement syringe drive Dispense accracy ±1% typical at 100 µl Dispense precision 1.5% CV typical at 100 µl Spply bottle volme 2 L Physical Characteristics Power Volts AC 50/60 Hz Dimensions Weight Reglatory Precision Instrment only: 21 W x 15 D x 16 H (525 x 374 x 400 mm) Instrment with optional cabinet: 23 W x 17.5 D x 17.5 H (575 x 435 x 435 mm) Instrment only: 28 lbs (12.7 kg) Instrment with optional cabinet: 38 lbs (17.2 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Weight Reglatory Instrment only: 40 lbs (18 kg) Instrment with optional cabinet: 64 lbs (29 kg) For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. Specifications are sbject to change. 59

60 60 BioTek Atomation Overview

61 Atomation Atomation prodcts provide speed, flexibility and nattended operation when configred with BioTek s line of microplate readers, washers and dispensers. The reslt is a scalable, cost-effective system that can adapt to yor changing reqirements. Additionally, BioStack prodcts can be integrated with most commercially available robotic arms to expand any atomated system s microplate capacity. 61

62 BioStack Microplate Stacker BioStack is a compact and versatile microplate stacker compatible with BioTek s microplate washers, dispensers, pipetting and detection systems. BioStack is easy to se and provides walk-away atomation for rotine processes. Ultra Fast Transfer Speeds The new BioStack3 offers the fastest plate transfer time, taking less than 10 seconds to remove and replace plates on the instrment carrier. BioStack3 is well-sited for high throghpt plate stacking reqirements with BioTek readers, washers and dispensers. 10-, 30-, or 50-Microplate Stacks Choose between 10-, 30-, or 50-plate stacks to best sit yor throghpt reqirements. Low volme, half-height plates are also compatible, with p to 75-plates capacity in the 50-plate stack. 96-, 384- and 1536-Well Microplate Handling BioStack is compatible with ANSI standard 96- and 384-well plates and low-volme 384-well plates may also be stacked, providing higher throghpt in a walk-away system. Compact, Rgged Design BioStack allows worry-free operation, even nder the heaviest sage. Motors, mechanical assemblies and software are all top qality for long term, continos se. The very small footprint allows BioStack and peripherals to fit within a biosafety cabinet or hood. Rotational Gripper The rotational gripper option optimizes positioning of the BioStack with its mating instrment, allowing the most ergonomically comfortable and efficient configrations. Barcode Scanner The optional barcode scanner can read and track plate IDs and send them to the plate data file in Gen5 or LHC Secre software for storage or export. Typical Applications: ELISA atomation Kinetic stdies Cell based assays Primary screening assays Colorimetric, florimetric and lminescence assays 62 BioTek Atomation BioStack

63 Specifications General BioStack3 Microplate types ANSI/SBS Standard and low profile 96-, 384- and wells General BioStack Microplate types ANSI/SBS Standard and low profile 96-, 384- and wells Microplate orientation Landscape and portrait Microplate orientation Landscape and portrait Microplate capacity 10, 30 and 50 plate stacks are removable and interchangeable 96-/384-well: Up to 50 plates 1536-well: Up to 75 plates Microplate capacity 10, 30 and 50 plate stacks are removable and interchangeable 96-/384-well: Up to 50 plates 1536-well: Up to 75 plates Compatible BioTek instrments EL406, 405 Toch, 405 LS, MltiFlo, PowerWave, Eon, Epoch R and Synergy Compatible BioTek instrments EL406, 405 Toch, 405 LS, MltiFlo, MicroFill, Precision, PowerWave, Eon, EpochR and Synergy Processing Speed (plate exchange time) <10 seconds Processing Speed (plate exchange time) <33 seconds Direct Control EL406, 405 Toch, 405 LS and MltiFlo can control BioStack3 from the keypad Direct Control EL406, 405 Toch, 405 LS, MltiFlo and MicroFill can control BioStack from the keypad PC Software Physical Characteristics LHC for liqid handling instrments (optional) Gen5 2.0 for readers Power Volts AC 50/60 Hz PC Software Physical Characteristics LHC for liqid handling instrments (optional) Gen5 2.0 for readers Power Volts AC 50/60 Hz Weight <25 lbs (11.3 kg) Weight <25 lbs (11.3 kg) Dimensions 7.4 W x 20.7 D (18.8 x 52.5 cm) Dimensions 7.0 W x 18.5 D (17.8 x 46.9 cm) Height when copled with: Height when copled with: EL406, 405 Toch, 405 LS, MltiFlo: 14.5 (36.8 cm) withot stacks 23.5 (59.6 cm) with 30-microplate stacks 33.5 (85.1 cm) with 50-microplate stacks EL406, 405 Toch, 405 LS, MltiFlo, MicroFill: 14 (35.6 cm) withot stacks 23 (58.4 cm) with 30-microplate stacks 33 (83.4 cm) with 50-microplate stacks Eon, Epoch, PowerWave, Synergy (except NEO): 16.5 (41.9 cm) withot stacks 25.5 (65.7 cm) with 30-microplate stacks 35.5 (90.1 cm) with 50-microplate stacks Eon, Epoch, PowerWave, Synergy (except NEO): 16 (40.6 cm) withot stacks 25 (63.5 cm) with 30-microplate stacks 35 (88.5 cm) with 50-microplate stacks Reglatory For In Vitro Diagnostic Use. All BioTek microplate instrmentation is CE and TUV marked Precision: 15 (37.0 cm) withot stacks 24 (61.1 cm) with 30-microplate stacks 34 (86.1 cm) with 50-microplate stacks Specifications are sbject to change. Reglatory For In Vitro Diagnostic se. All BioTek microplate instrmentation is CE and TUV marked. 63

64 Compliance As an ISO certified manfactrer, BioTek nderstands the importance of standardized prodct qalification procedres and traceability and provides a nmber of tools and services designed to streamline the process and minimize the resorces reqired to perform sch testing. 21 CFR Part 11 Compliance Prodcts Gen5 Secre Data Analysis Software offers a nmber of featres to ensre yor microplate reader system meets the reqirements of 21 CFR Part 11. LHC Secre protocol definition and control software for BioTek s microplate washers and dispensers is niqely designed to help ensre compliance to 21 CFR Part 11. Both software programs offer important secrity featres, inclding: Electronic signatre of data and protocol files Secre data storage Mltiple and definable ser permission levels Data and protocol adit trails Protected fnctions Prodct Qalification Software Validation A Validation Package is available for Gen5 Software to allow testing and validation of key fnctions within Gen5 and Gen5 Secre. Inclded in the easy-to-se package are: Test Plans Reslts Checklists Data sets Instrment IQ/OQ/PQ Packages BioTek offers a complete men of Prodct Qalification Packages for all of or microplate instrments. All prodct Qalification Packages are flly validated to assre that the procedres and associated data/spreadsheets spplied in the package meet reglatory reqirements. Within each package, yo ll find detailed: Prodct Specifications Qalification interval gidelines IQ/OQ/PQ test plans and procedres Data sets (where applicable) Qalification checklists and log sheets for complete docmentation 64 BioTek Compliance

65 Test Plates The se of standardized plates to spplement the verification an instrment s performance is a timeand resorce-saver in most laboratory environments. BioTek offers several test plates to facilitate the test procedres fond in or microplate reader IQ/OQ/PQ packages, and can be atomated throgh the Gen5 software. Absorbance Test Plates: For se with the ELx800, ELx808, Eon, Epoch, PowerWave HT and for the absorbance mode of the Synergy line of mlti-mode readers. Ensre GxP compliance by checking instrment performance against specifications for: Accracy Repeatability Linearity Wavelength accracy (for monochromator-based systems) Florescence Test Plates: Ideal for qick checks of the florescence intensity detection system between more thorogh instrment qalification. The Florescent test plate aids in maintaining GxP compliance by atomatically checking a series of critical performance parameters, inclding: Alignment Cross talk Signal-to-noise ratio Linearity Precision Lminescence Test Plates: NIST-traceable lminescence test plate is sed with the applicable Prodct Qalification Package or pdated User s Manal. Featres of the Lminescence Test Plate inclde: NIST-traseability certificate inclded, garantees a controlled light otpt from the test plate Simple design, easy to se: jst trn the plate on, and read the ltra-stable, low light level LEDs Test Plate Recertification Programs are available, contact BioTek Service for details. BioTek Compliance 65

66 Applications Spport At BioTek, or cstomers applications come first. Whether it s or existing prodcts or new prodcts in development, we design in capabilities to enable yor most important applications. To facilitate this process, BioTek has an on-site Applications Lab with a team of seasoned scientists continosly working on ctting-edge scientific applications and partnering with the best-known reagent and kit vendors. Below are some examples of application areas where we have demonstrated the tility of or prodcts. Small Molecle Drg Discovery Celllar signaling Screening assays Biochemical assays Cell-based assays Selectivity profiling In vitro ADME/Tox Biologics/Biosimilars Clonal selection ADCC assays Immnogenicity Bridging assays Biomolecle Qantification Ncleic acid & protein (microplate and micro-volme) Immnoassays (ELISA, AlphaLISA, etc.) Mltiplexed xmap assays Food & Beverage Food safety testing Food sorce screening and monitoring Ingredient and ntritional testing Process monitoring Netralization assays Aggregation Biofel Research Celllosic ethanols Life Science Research Algal lipid prodction Signal transdction assays Live cell analysis Reactive oxygen species Kinetic assays Genotyping BioTek jornal pblications, application notes, poster presentations and citations are available at: 66 BioTek Applications Spport

67 Global Service & Spport Or teams are committed to providing the service and spport yo need to sstain optimal performance of yor BioTek prodcts. BioTek Scientists, Engineers, Technicians and Sales Representatives throghot the world provide valable assistance to laboratories worldwide. Technical Spport Center Immediate access to or team of highly skilled Service Engineers Complete technical assistance for instrmentation, software Training Worldwide technical training soltions Cstomized to meet yor needs Experienced BioTek instrctors and applications Cstomer Resorce Center (CRC) Service Centers Highly experienced Service Technicians Test Plate Certification, Dispense Cassette Calibration, Preventive Maintenance, Repair, Software and Instrment Upgrades Field Service Field Service personnel are available to visit yor site Installation, Installation Qalification, Operational Qalification, Preventive Maintenance, Repair, Training, Software and Instrment Upgrades BioTek s Cstomer Resorce Center gives cstomers access to information abot their specific BioTek microplate instrmentation and software. This web site makes it easy for cstomers to acqire relevant and necessary information. Cstomers can: Track orders and check prodct stats Maintain eqipment inventory Access warranty information Download technical information, ser manals and software pdates Reqest service and technical spport Access to BioTek s Cstomer Resorce Center and more details on BioTek s service and spport are available at BioTek Global Service & Spport 67