Scalability of the Mobius CellReady Single-use Bioreactor Systems
|
|
- Jack Walters
- 6 years ago
- Views:
Transcription
1 Application Note Scalability of the Mobius CellReady Single-use Bioreactor Systems Abstract The Mobius CellReady single-use bioreactor systems are designed for mammalian cell growth and recombinant protein production. The Mobius CellReady product offering includes bench-scale (3 L), small-scale (5 L) and pilot-scale (2 L) bioreactor systems spanning early process development through clinical batch production. To enable the successful scale-up of a biomanufacturing process, a number of factors critical to efficient cell growth, viability and protein production were considered and used to develop an optimized design. These include mixing efficiency, gas transfer capability, and an operating range of power input that minimizes cell shear. In this study, several key design parameters, such as oxygen mass transfer coefficient (k L a), power per unit volume, Reynolds number (Re), mixing time and tip speed were characterized for the three different sized single-use bioreactor process containers. Based on these data, CHO cells were cultured in each of these bioreactor systems maintaining equivalent power per unit volume as the primary scaling parameter. These data provide evidence of cell culture scalability across the entire Mobius CellReady single-use bioreactor platform. Figure 1A. 3 L Mobius CellReady Bioreactor Introduction The Mobius CellReady family of products includes the bench-scale (3 L), small-scale (5 L) and pilot-scale (2 L) bioreactor systems that enable cell growth in volumes appropriate for early process development through clinical batch production. The small-scale Mobius CellReady 3 L bioreactor is a rigid stirred tank bioreactor (Figure 1A) while the Mobius CellReady 5 L and 2 L bioreactor systems (Figures 1B and 1C) are inflatable stirred tank bioreactor process containers used in stainless steel vessels. Table 1 outlines the features of all three. Both the Mobius CellReady 5 L and 2 L bioreactor process containers utilize the novel Mobius SensorReady technology for process monitoring and control. The Mobius SensorReady assembly is an external loop that is connected to the bioreactor process container that allows for a configurable number of probes and sensors to be used. Figure 1B. 5 L Mobius CellReady Bioreactor EMD Millipore is a division of Merck KGaA, Darmstadt, Germany Figure 1C. 2 L Mobius CellReady Bioreactor
2 Table 1: Parameters 3 L 5 L 2 L Total Volume (L) Minimum Working Volume (L) Maximum Working Volume (L) Total Height to Diameter Ratio (nominal) 1.8:1 2.:1 2.:1 Liquid Height to Diameter Ratio 1.4:1 1.6:1 1.6:1 (at max working volume) Vessel Diameter (cm) Impeller Diameter (cm) Impeller Geometry Up-pumping marine (3 blades) Up-pumping pitched blade (4 blades at 13 pitch) Impeller Position On shaft, centered Bottom mount, 15 from center Impeller Power Number (Np) Baffles NA 1 vertical baffle Open Pipe Sparger Orifice 2.3 mm 1.5 mm Microsparger Type Sintered polyethylene Membrane polyethylene Vessel Heating Electric heating blanket Stainless steel liquid jacket Control 3 probe ports Mobius SensorReady Technology Fits standard 12 mm PG 13.5 threaded probes The ability to scale up a biomanufacturing process is essential for process development and the production of biologics. Bioreactor process set points, acceptable ranges and general operating parameters used at the large scale are commonly based on those developed at the benchtop or small scale where experimentation is more cost effective and efficient. Large-scale performance and production expectations are often established based on results obtained at smaller scales. It is therefore important that the process parameters developed at the small scale are readily transferrable to the larger scale. Challenges to bioreactor scale-up occur because even when two geometrically similar tanks are used it is not possible to simultaneously maintain key bioreactor characteristics such as shear, mixing time, and k L a identical in both the large and small tanks. 1 Other variables, such as bubble size and distribution, nutrient regulation and delivery, and process control capabilities may also contribute to variable performance results across scales. Ultimately, successful scale-up is determined when comparable process performance endpoints such as cell growth, cell viability, protein production, and product quality are achieved. The probability of meeting these criteria can be increased when the bioreactor systems are well-characterized and the process design space is better understood. In this study, several key engineering parameters such as oxygen mass transfer coefficient (k L a), power per unit volume, Reynolds number (Re), mixing time and tip speed were characterized for the three different sized single-use bioreactor process containers. CHO cells were then cultured in each of these bioreactor systems based on maintaining equivalent power per unit volume as the primary scaling parameter. The results of this study define the characterization and process design space offered by the bioreactor systems and demonstrates the capability to achieve expected cell culture performance results across scales, thus demonstrating the scalability of the family of Mobius CellReady bioreactor systems.
3 Results Efficient Gas Transfer One of the most critical scale-up parameters for bioreactors is mass transfer of gasses. In order to provide an optimal environment for the cells, a bioreactor must be able to supply enough dissolved oxygen (O 2 ) for efficient cellular metabolism as well as maintain an appropriate level of dissolved carbon dioxide (CO 2 ). Sufficient O 2 /Air delivery is required not only to support cell growth and protein production but also to prevent excessive CO 2 accumulation in the media which can impact both of these critical performance endpoints. 2 To assess the gas transfer efficiency of the Mobius CellReady bioreactor process containers, the volumetric mass-transfer coefficients (k L a) for oxygen were measured in each bioreactor process container using the static gassing out method. k L a values were determined by filling the bioreactor process containers to the maximum working volume with a mock media (1X PBS, 2 g/l Pluronic F-68, 5 ppm Anti-foam C), and setting the temperature to 37 C. The dissolved oxygen was stripped from the bioreactors by supplying nitrogen gas via the microsparger. Once the dissolved oxygen (DO) concentration reached less than 2% air saturation, the nitrogen supply was turned off and air was sparged through the microsparger at flow rates ranging from.25 vvm to.5 vvm at two different agitation rates. The DO concentration was recorded over time until the dissolved oxygen concentration in the media plateaued at the fully saturated value (1% air saturation). An air overlay gas was not used in these studies. k L a Calculation: The k L a value for each trial was calculated from the linear portion of the DO vs. time graph. To avoid subjectivity in determining the linear portion, the uniform DO interval used for the calculation was between 1% and 9% air saturation. The k L a values shown represent the slope of the line created by plotting ln((c*-ct 1 )/(C*- Ct 2 )) versus time (t 2 -t 1 ), where C* is fully saturated liquid, Ct 1 is the percent air saturation at the initial time, Ct 2 is the percent air saturation at time 2, and t 1 and t 2 are the initial time and time 2 respectively. As detailed in Table 1, the impeller and sparger design and placement in the 5 L and 2 L bioreactor process containers are similar; however the 3 L bioreactor is significantly different from the 5 L and 2 L bioreactor process containers. For example, in the 5 L and 2 L bioreactor process containers the membrane polyethylene microsparger is located directly beneath the impeller, while in the 3 L bioreactor a sintered polyethylene microsparger is located off to the side of the impeller. Also, the impeller blade shape differs between the bioreactors. A pitched blade impeller is found in the 5 L and 2 L bioreactor process containers while a marine impeller is found in the 3 L bioreactor. As shown in Figure 2A, the 5 L and 2 L bioreactor process containers, at the same power per unit volume, exhibit comparable k L a values. These two bioreactor process containers can achieve k L a values ranging from 4 hr -1 at the lowest agitation and air flow rates tested to 6 hr -1 at the highest agitation and air flow rates tested. Given the differences in the volume and design between the 3 L bioreactor and the 5 L and 2 L bioreactor process containers, it is not surprising that, at the same gas flow rates and power per unit volume, the k L a values are not immediately comparable. However as shown in Figure 2B, by adjusting the air flow rates, scalable k L a values between all three bioreactor systems at similar power per unit volumes can be achieved. k L a (hr -1 ) k L a (hr -1 ) L 5 L.25 vvm.1 vvm.2 vvm.5 vvm.25 vvm.1 vvm.2 vvm.5 vvm 1 W/m 3 1 W/m 3 3 L 2 W/m 3 14 W/m 3.5 vvm.1vvm.2 vvm.5 vvm.5 vvm.1vvm.2 vvm.5 vvm 2 L 5 L 3 L.25 vvm.1 vvm.2 vvm.5 vvm.25 vvm.1 vvm.2 vvm.5 vvm 1 W/m 3 1 W/m 3 5 L and 2 L Figure 2A. k L a Scalability of the 5 L and 2 L Mobius CellReady Bioreactor Process Containers. Each bar represents the average k L a value of n=3 at each air flow rate and impeller agitation rate tested. Error bars represent the standard deviation of triplicate determinations. Figure 2B. k L a Scalability of the family of Mobius CellReady Bioreactor Systems. Each bar represents the average k L a value of n=3 at each air flow rate and impeller agitation rate tested. Error bars represent the standard deviation of triplicate determinations.
4 Mixing Mixing is a critical bioreactor performance characteristic because it is responsible for minimizing gradients and maintaining control within the cell culture environment. Good mixing in a bioreactor strives for sufficient fluid pumping and turnover throughout the system to effectively create a single homogeneous environment which can be accurately monitored and controlled. Good mixing should evenly distribute bioreactor contents, helping to minimize zones of uneven cell density, ph, temperature, dissolved gases and nutrient or waste concentrations, while minimizing the shear stress imparted on the cells by the fluid dynamics or the mixing element itself. Although no single parameter can guarantee comparable process performance between stirred tank bioreactor systems, choosing an agitation rate that matches energy dissipation or power per unit volume (W/m 3 ) is a common first approach. 5,6 The impeller design, fluid density and agitation rate are considered in the power per unit volume (P o /V) equation, P o /V = Np x ρ x n 3 x d 5, where P o is ungassed power, V is liquid volume, Np is impeller power number, d is impeller diameter, n is impeller agitation rate and ρ is fluid density. Using this scaling method, agitation rate is varied to maintain similar ungassed power per unit volume across vessels. The highest shear zones in a stirred tank bioreactor are often described as existing within the impeller zone. Because the outer edge of the impeller blades create shear as they rotate through the liquid, the impeller tip speed is often considered during bioreactor comparisons. The impeller tip speed calculation, π x d x n, where d is impeller diameter and n is agitation rate, however, does not take impeller design into consideration. Reynolds number (Re) can be considered when estimating fluid conditions at a given agitation rate by providing a ratio of the inertial forces to the viscous forces where Re = (ρ x n x d 2 ) / m. The fluid density is represented by ρ, n is agitation rate, d is impeller diameter and m is dynamic viscosity. The system is considered fully turbulent when Re > 1,. 3,4 The Reynolds number calculation is based on the assumption of a cylindrical tank with a centered rotating impeller and does not take impeller design into account. The Mobius CellReady 5 L and 2 L bioreactor process containers contain a bottom mounted, offset, pitched blade impeller and a baffle, which together increase the turbulence and improve overall mixing.
5 Tables 2, 3 and 4 below outline various tip speed, Reynolds number and ungassed power per unit volume values calculated for a range of applicable agitation rates for the 3 L, 5 L, and 2 L Mobius CellReady bioreactor process containers. Please refer to the Mobius CellReady 5 L and 2 L Single-use Bioreactor Systems Performance Guide for more information regarding the determination of impeller power number. Table 2: 3 L Bioreactor Process Container (Np =.3) rps rpm tip speed (m/s) W/m 3 Re , , , , , , ,258 Table 3 5 L Bioreactor Process Container (Np = 3.2) rps rpm tip speed (m/s) W/m 3 Re , , , , , , , ,281 Table 4 2 L Bioreactor Process Container (Np = 4.) rps rpm tip speed (m/s) W/m 3 Re , , , , , , , ,257 Mixing was evaluated for the 5 L and 2 L Mobius CellReady bioreactor process containers by observing conductivity probe response curves measured at four locations (top, middle, bottom and inserted in the probe port of the Mobius SensorReady loop) within the system. The average of these four probes is considered the system average. A salt solution was introduced at the liquid surface and the T 95 mixing time was determined for each of the four probe locations as the time when conductivity profiles had reached 95% of its final value. Each trial was performed in triplicate and results are shown in Figure 3. The Mobius CellReady 3 L bioreactor was evaluated in a similar manner, using just one conductivity probe inserted in the head plate probe port. Mixing Time (sec) L 5 L 3 L Power / Volume (W/m 3 ) Results of these studies show that the Mobius CellReady 5 L and 2 L bioreactor process containers demonstrated similar system average mixing times at equivalent power per unit volume. Mixing times for the 3 L bioreactor are significantly shorter than mixing times observed in the larger bioreactor process containers for similar power per unit volume. This is to be expected as larger liquid volumes result in longer fluid paths. In order to achieve a well mixed condition in the same amount of time, the fluid velocity would have to increase for the condition in the larger volume tank. As a guide, fluid velocity is proportional to the square root of power per unit volume in turbulent conditions (ie, when Re > 1,). 3,4 As a result, an increase in power per unit volume would be required to achieve similar mixing times at significantly larger scales. The system average mixing times for the Mobius CellReady bioreactor process containers, as determined by the multipleprobe experimental methods described above, do not dramatically increase with increasing power per unit volume across the agitation range tested. The 5 L and 2 L bioreactor process containers contain a single baffle, which coupled with the off-center, angled position of the bottom mounted impeller, provides good mixing dynamics even at lower power inputs. Empirical results show the mixing time to be seconds from 2 to 15 W/m 3 for the 3 L, seconds from 5 to 3 W/m 3 for the 5 L and seconds from 1 to 25 W/m 3 for the 2 L bioreactor process containers as displayed in Figure 3. Please refer to the Mobius CellReady 5 L and 2 L Single-Use Bioreactor Systems Performance Guide for more details on mixing characterization. Figure 3. System Average Mixing Times of the family of Mobius CellReady Bioreactor Systems. Data points represent the system average mixing time of n=15 individual trials. Error bars represent 1 standard error for each data point
6 Cell Culture Maintaining a homogenous environment within the bioreactor is the most critical criterion for a successful cell culture process. While different agitation strategies may be used to scale up a biomanufacturing process, power per unit volume is the most often used scaling parameter. 5,6 The second most important criterion for a successful cell culture run is efficient delivery of oxygen to maintain cell growth and productivity. Using these two criteria, a CHO cell culture batch process was performed in the 3 L, 5 L and 2 L bioreactor systems using power per unit volume as the primary scaling parameter to demonstrate cell culture scalability across the Mobius family of bioreactor systems. Further, gas flow rates were chosen to achieve similar k L a values for each vessel. The process parameters for each scale are outlined in Table 5. Table 5 Operating Parameters 3 L Mobius CellReady System 5 L Mobius CellReady System 2 L Mobius CellReady System Agitation Rate 2 rpm (14 W/m 3 ) 147 rpm (14 W/m 3 ) 91 rpm (14 W/m 3 ) Controller Applikon EZ Controller Finesse G3 PC System Finesse Delta V RDPD System Air Overlay None.1 vvm.1 vvm Dissolved Oxygen 3% (Air sparge on/off,.1 vvm) 3% (Air/O 2 continuous PID,.2 vvm) ph 6.95 ±.5 with CO 2 and.5m Na 2 CO 3 Temperature 37 C Media OptiCHO, 15% Efficient Feed A, 15% Efficient Feed B, 4 mm Glutamine, 5 ppm Anti-foam, 2 g/l Pluronic F-68 Target Seed Density.2 x 1 6 cells/ml
7 To demonstrate scalable cell culture performance, parameters including cell growth, viability and metabolism were compared. For this study, samples were analyzed daily on a Vi-Cell XR (Beckman Coulter), a BIOPROFILE FLEX system (NOVA Biomedical) and a Blood Gas Analyzer (Siemens Rapidlab 248). As shown in Figure 4A, the viable cell densities and viabilities were comparable between all three scales. As shown in Figure 4B and 4C, the nutrient profiles and metabolic rates between all three scales were also comparable. The comparable cell growth, viabilities and metabolic profiles demonstrate that the cell culture performance in all three Mobius CellReady bioreactor systems is scalable Viable Cell Density (1 6 cells/ml) Days in Culture Cell Viability (%) Figure 4A. Viable cell density and viability vs. culture time for a batch culture process at the 2 L, 5 L and 2 L working volumes. Data was obtained daily from the Vi-Cell XR (Beckman Coulter). Mobius CellReady 2 L Mobius CellReady 5 L Mobius CellReady 3 L Glucose (g/l) Days in Culture Lactate (g/l) Figure 4B. Glucose and lactate concentrations vs. culture time for a batch culture process at the 2 L, 5 L and 2 L working volumes. Data was obtained daily from the BIOPROFILE FLEX system (NOVA Biomedical) Figure 4C. g/(1 9 cell-day) Mobius CellReady 3 L Mobius CellReady 5 L Mobius CellReady 2 L Glucose Consumption Lactate Procution Metabolic rates for culture days 1 5 for a batch culture process at the 2 L, 5 L and 2 L working volumes. Data was obtained daily from the BIOPROFILE FLEX system (NOVA Biomedical).
8 Conclusions Successful scale-up of a biomanufacturing process is dependent on several factors including gas mass transfer, mixing efficiency and shear effects. Through a series of experiments aimed at characterizing several key engineering parameters of the bioreactors, we have developed an in-depth understanding of the process design space of the three Mobius CellReady bioreactor systems. We have demonstrated that the three bioreactor systems, despite design and volume differences, are capable of achieving equivalent k L a values by adjusting the air flow rates at equivalent power per unit volume set points. We have also demonstrated that similar system average mixing times can be achieved for the 5 L and 2 L systems within their power per unit volume operating range. Based on our understanding of the design space of each of the bioreactor systems, process set points were chosen for CHO cell cultivation in each of these. Maintaining equivalent power per unit volume was chosen as the primary scaling parameter and gas flow rates were chosen based on achieving similar k L a values at each scale. We demonstrated that during a ten day batch culture, comparable cell growth, viability, and nutrient metabolism were achieved in each bioreactor system. The comparable cell culture performance results with all three bioreactor systems demonstrate the scalability of the family of Mobius CellReady bioreactor systems. We have shown that the comprehensive characterization of several key engineering parameters resulted in a detailed understanding of the design space of each bioreactor. This allows users to choose process set points that enable successful scale-up of biomanufacturing process across the Mobius CellReady family from the 3 L to 2 L scale. References 1. Shuler, M., and Kargi, F. (22). Bioprocess Engineering Basic Concepts, 2nd ed., Prentice Hall, Inc. 2. Marks, D.M. (23). Equipment design considerations for large scale cell culture. Cytotechnology 42: Dalian University of Technology. Mixing, Chapter 6. ceb.dlut.edu.cn/uploads/soft/11415/ pdf 4. Nienow, A.W. (26). Reactor engineering in large scale animal cell culture. Cytotechnology 5: Xing, Z. et al. (29). Scale-up Analysis of a CHO Cell Culture Process in Large-Scale Bioreactors. Biotechnology and Bioengineering 13: Kaiser, S.D. et al. (211) CFD for Characterizing Standard and Single-use Stirred Cell Culture Bioreactors. In Computational Fluid Dynamics Technologies and Applications, InTech. To Place an Order or Receive Technical Assistance In the U.S. and Canada, call toll-free 1(8) For other countries across Europe, call +44 () For other countries across Europe and the world, please visit For Technical Service, please visit /offices EMD Millipore, the M mark, and Mobius are registered trademarks of Merck KGaA, Darmstadt, Germany. Vi-CELL is a trademark of Beckman Coulter, Inc. BIOPROFILE is a registered trademark of NOVA Biomedical Corporation. Rapidlab is a registered trademark of Siemens Corporation. Lit No. AN4228EN Rev. B PS SBU Printed in the USA. 5/ EMD Millipore Corporation, Billerica, MA USA. All rights reserved.
Single-Use Simplicity
Single-Use Simplicity BioBLU c and BioBLU p Single-Use Vessels for cell culture »Proven stirred-tank design meets single-use technology.«reliable performance and ease-of-use Combine the benefits of single-use
More informationXDR Single-Use Bioreactors
DATA SHEET XDR Single-Use Bioreactors Introduction The XDR Single-Use Bioreactor is a fully-integrated system that delivers proven stirred-tank performance across a wide range of cell lines. Single use
More informationA Hands-On Guide to Ultrafiltration/ Diafiltration Optimization using Pellicon Cassettes
Application Note A Hands-On Guide to Ultrafiltration/ Diafiltration Optimization using Pellicon Cassettes In ultrafiltration (UF) tangential flow filtration (TFF) systems, operating parameter selection
More informationScale-up & scale-down between the two. worlds of shaken and stirred bioreactors
Scale-up & scale-down between the two worlds of shaken and stirred bioreactors Prof. Dr.-Ing. Jochen Büchs AVT - Biochemical Engineering, RWTH Aachen University Sammelbau Biologie, D - 52074 Aachen, Germany
More information6.1 Mixing Equipment. Fig. 6.1 A standard tank with a working volume of 100 M 3 and used for penicillin production
Chapter 6 Mixing Mixing, a physical process which aims at reducing non-uniformities in fluids by eliminating gradients of concentration, temperature, and other properties, is happening within every bioreactor.
More informationThermo Scientific HyClone Single-Use Bioreactor Products and Capabilities. Discovery Development Production
Thermo Scientific HyClone Single-Use Bioreactor Products and Capabilities Discovery Development Production Introduction Leading the way in Single-Use Bioreactors Since its introduction, the Thermo Scientific
More informationScaling Down Bioreactor Process Development: Comparison of Microbioreactor and Bench Scale Solutions.
Scaling Down Bioreactor Process Development: Comparison of Microbioreactor and Bench Scale Solutions. Richard Lugg. Scientist I, MedImmune. European Laboratory Robotics Interest Group: High Throughput
More informationEfficient and controlled expansion of IgG1 producing CHO-DG44 cells using the ActiCHO Media System and WAVE Bioreactor
Efficient and controlled expansion of IgG1 producing CHO-DG44 cells using the ActiCHO Media System and WAVE Bioreactor Thomas Falkman, Eric Fäldt, Anita Vitina and Cecilia Annerén. GE Healthcare Bio-Sciences
More informationF1 1/10 R&D BIOREACTORS/FERMENTORS
F1 1/10 R&D BIOREACTORS/FERMENTORS THE COMPANY Bionet is a specialist in Bioprocesses Engineering. We provide equipment (Bioreactors, Cross-Flow Filtration Systems and Cleaning-In-Place Systems) and advanced
More informationF2 15/30 SIP PILOT SCALE BIOREACTORS
F2 15/30 SIP PILOT SCALE BIOREACTORS THE COMPANY Bionet is a specialist in bioprocesses engineering. We provide equipment (Bioreactors, Cross-Flow Filtration Systems and Cleaning-In-Place Systems) and
More informationKGC SCIENTIFIC FERMENTER DESIGN INDUSTRIAL SCALE
KGC SCIENTIFIC www.kgcscientific.com FERMENTER DESIGN INDUSTRIAL SCALE Definition of Biotechnology Utilization of bioprocess using microorganism, plant tissue, and animal cell, and components of them for
More informationOxygen Transfer Performance of Unbaffled Stirred Vessels in View of Their Use as Biochemical Reactors for Animal Cell Growth
A publication of CHEMICA ENGINEERING TRANSACTIONS VO. 27, 2012 Guest Editors: Enrico Bardone, Alberto Brucato, Tajalli Keshavarz Copyright 2012, AIDIC Servizi S.r.l., ISBN 978-88-95608-18-1; ISSN 1974-9791
More informationFermenter System Bioreactor Lab / Pilot / Industrial Fermenter Automation Fermenter System
Fermenter System Bioreactor Lab / Pilot / Industrial Fermenter Automation Fermenter System About us CNS Co., Ltd. has reached exclusive position in fermenter development, design, and manufacturing area.
More informationAvailable online Research Article. Reactor design strategy: Production of xanthan from sugarcane broth
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(5):323-329 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Reactor design strategy: Production of xanthan from
More informationIn the biopharmaceutical industry,
S i n g l e - U s e TECHNOLOGIES Continuous Cell Culture Operation at 2,000-L Scale Michael Sherman, Vincent Lam, Melissa Carpio, Nick Hutchinson, and Christel Fenge In the biopharmaceutical industry,
More informationTECHNICAL PAPER. Stirred Bioreactor Engineering for Production Scale, Low Viscosity Aerobic Fermentations: Part 1. By: Dr.
TECHNICAL PAPER Stirred Bioreactor Engineering for Production Scale, Low Viscosity Aerobic Fermentations: Part 1 By: Dr. Alvin Nienow Senior Technical Consultant The Merrick Consultancy Merrick & Company
More informationParameters to Consider When Expanding Cells on Corning Microcarriers
Parameters to Consider When Expanding Cells on Corning Microcarriers Application Note Katherine E. Strathearn, Ph.D. and Ana Maria P. Pardo Corning Incorporated, Life Sciences Kennebunk, Maine 04043 Introduction
More informationA ceramic microsparging aeration system for cell culture reactors
Publication Series of IBPT University of Applied Sciences Giessen-Friedberg No. 1, 2 A ceramic microsparging aeration system for cell culture reactors Peter Czermak* 1, Christian Weber, Dirk Nehring Institute
More informationIntegrated Single-Use Bioreactor (S.U.B.) systems with controllers
Integrated Single-Use Bioreactor (S.U.B.) systems with controllers Providing simplicity in operation Integrated Single-Use Bioreactor and controller systems Our integrated single-use bioreactors are designed
More informationComparative Studies on Scale-Up Methods of Single-Use Bioreactors
Utah State University DigitalCommons@USU All Graduate Theses and Dissertations Graduate Studies 5-2011 Comparative Studies on Scale-Up Methods of Single-Use Bioreactors Emily B. Stoker Utah State University
More informationThermo Scientific HyClone Single-Use Bioreactor (S.U.B.) 1000 L
Thermo Scientific HyClone Single-Use Bioreactor (S.U.B.) 1000 L The Thermo Scientific HyClone Single-Use Bioreactor (S.U.B.) is the leading single-use alternative to conventional stirred tank bioreactors
More information7 Liter BioBundle System Complete fermentation system for microbial and cell culture applications
7 Liter BioBundle System Complete fermentation system for microbial and cell culture applications COMPLETE A U T O FERMENTATION C L A V A B L E SSYSTEM Y S T E MFOR S MICROBIAL AND CELL CULTURE APPLICATIONS
More informationWe Know Bioprocessing
In touch with life We Know Bioprocessing Parallel Cultivation of Microorganisms using Single-use Mini Bioreactors Bio Base NWE Workshop & CLIB-Forum Scale-up & Scale-down Creative Campus Monheim, Germany
More informationbio-t mini The Laboratory Bioreactor System
bio-t mini The Laboratory Bioreactor System Adress: zeta Bio- und Verfahrenstechnik Alte Jonastrasse 83 CH-8640 Rapperswil Switzerland Contact: Dr. Michael Nägeli Phone: +41 55 450 60 06 Fax: +41 55 460
More informationBioreactor System ERT 314. Sidang /2012
Bioreactor System ERT 314 Sidang 1 2011/2012 Chapter 3:Types of Bioreactors Week 4-5 Handouts : Chapter 13 in Doran, Bioprocess Engineering Principles Background to Bioreactors The bioreactor is the heart
More informationBCT Loop Reactor Technology
BCT Loop Reactor Technology By BUSS ChemTech AG www.buss-ct.com Hohenrainstrasse 10 CH-4133 Pratteln 1, Switzerland Tel. + 41 (0) 618 256 462 Fax. +41 (0) 618 256 737 Abstract This paper highlights the
More informationMANGANESE REMOVAL FROM COBALT SOLUTIONS WITH DILUTE SULPHUR DIOXIDE GAS MIXTURES
MANGANESE REMOVAL FROM COBALT SOLUTIONS WITH DILUTE SULPHUR DIOXIDE GAS MIXTURES J van Rooyen, S. Archer and M. Fox Senior Process Engineer TWP Matomo Process Plant P.O. Box 5100 Rivonia, 2128 Senior Process
More informationIN SITU STERILIZABLE BIOREACTORS
IN SITU STERILIZABLE BIOREACTORS Diachrom Biotechnology team with over 20 years experience in sterile process engineering and bioprocess technologies has realised a complete range of laboratory and pilot
More informationIN SITU STERILIZABLE BIOREACTORS
IN SITU STERILIZABLE BIOREACTORS Solida Biotechnology team with over 20 years experience in sterile process engineering and bioprocess technologies has realised a complete range of laboratory and pilot
More informationPerformance Evaluation and Cleanability Study using Pellicon 3 Cassettes with 30 kd Biomax and Ultracel Ultrafiltration Membranes
Application Note Performance Evaluation Cleanability Study using Pellicon Cassettes with kd Biomax Ultracel Ultrafiltration Membranes Consistent, high performance ultrafiltration throughout multiple process
More informationDuring the past few years, use
D I S P O S A B L E S BIOREACTORS Bridging the Gap from Reusable to Single-Use Manufacturing with Stirred, Single-Use Bioreactors A Development Approach Based on the Gold Standard Davy De Wilde, Ute Noack,
More informationIn the current competitive biopharmaceutical
Image Courtesy of Sartorius Stedim Biotech single-use Bioreactors for the rapid Production of Preclinical and clinical Biopharmaceuticals Rüdiger Heidemann, Christopher R. Cruz, Paul Wu, Mikal Sherman,
More informationBioreactor Components
August 2017 Fully stocked and ready to ship! Bioreactor Components Available in 2, 3, 5, 7 and 15L Sizes Like-for-like replacement parts for Applikon Biotechnology and Broadley-James brand bioreactors.
More informationChapter 7 Mass Transfer
Chapter 7 Mass Transfer Mass transfer occurs in mixtures containing local concentration variation. For example, when dye is dropped into a cup of water, mass-transfer processes are responsible for the
More informationEFFECTS OF SHEAR AND CAVITATION ON PARTICLE AGGLOMERATION DURING HANDLING OF CMP SLURRIES CONTAINING SILICA, ALUMINA, AND CERIA PARTICLES
EFFECTS OF SHEAR AND CAVITATION ON PARTICLE AGGLOMERATION DURING HANDLING OF CMP SLURRIES CONTAINING SILICA, ALUMINA, AND CERIA PARTICLES Mark R. Litchy and Donald C. Grant CT Associates, Inc. Reto Schoeb
More informationSimulating Process Limitations in Microbial Cultivation: A Parallel Two-Compartment Scale-Down Approach
APPLICATION NOTE No. 301 I February 2016 Simulating Process Limitations in Microbial Cultivation: A Parallel Two-Compartment Scale-Down Approach Michael H. Limberg 1, Stephan Zelle 2, Christiane Schlottbom
More informationCritical Analytical Measurements for Bioreactor Optimization. controlling an organism s chemical environment leads to consistent and
Critical Analytical Measurements for Bioreactor Optimization Mettler-Toledo Ingold, Inc., Bedford, MA Abstract Most bioreactor processes share a basic principle; optimizing and controlling an organism
More informationStatic Mixers Applications Working Principle Sizing your Static Mixer Overview Production
VERDERMI STATIC static mixers MIERS Static Mixers Applications Working Principle Sizing your Static Mixer Overview Production Verdermix Static Mixers The Verdermix series Static Mixers is a well balanced
More informationCase study: Tech transfer of mammalian cell culture process from lab scale to production scale using disposable technology
Case study: Tech transfer of mammalian cell culture process from lab scale to production scale using disposable technology Dawid Suwała aa. Mammalian Drug Substance Manufactruring Manager Polpharma Biologics
More informationFlow and Heat Transfer Characteristics in High Porosity Metal Foams
Proceedings of the World Congress on Mechanical, Chemical, and Material Engineering (MCM 2015) Barcelona, Spain July 20-21, 2015 Paper No. 333 Flow and Heat Transfer Characteristics in High Porosity Metal
More informationAPlus-QMC LLC. Catalog 2017
APlus-QMC LLC Catalog 2017 A+FLOWTEK Balanced Flow Meter NASA 2006 Invention of the Year NASA 2010 Award for Excellence in Technology Transfer NASA 2010 and 2011 Nominee for Space Technology Hall of Fame
More informationDesign and characterisation of a parallel miniaturised bioreactor system for mammalian cell culture
Design and characterisation of a parallel miniaturised bioreactor system for mammalian cell culture A thesis submitted to University College London for the degree of Doctor of Engineering By Omar Al-Ramadhani
More informationEXPERIMENTAL AERODYNAMICS FLOW TROUGH AN ORIFICE LAB SHEET
EXPERIMENTAL AERODYNAMICS FLOW TROUGH AN ORIFICE LAB SHEET EMRE KOÇ ONUR GÜNEL 1- Introduction When a fluid passes through a constriction, such as through a sharp-edged hole or over a weir, the constriction
More information2/22/2011. Presentation Outline. Overview of Wastewater Aeration. Basic Equation. Some Acronyms. dc dt. dc dt TDS BP T C L
Presentation Outline Overview of Wastewater Aeration Randal W. Samstag, P.E. Carollo Engineers 1. Some definitions 2. Types of aeration devices 3. What affects aeration efficiency? 4. How is aeration efficiency
More informationEden Approach To Bioprocess Characterisation. Philip Mellors p USP Development & Transfer Team Leader
Eden Approach To Bioprocess Characterisation Philip Mellors p USP Development & Transfer Team Leader Process Characterisation What is Process Characterisation? A late stage study that evaluates the process
More informationFermentation monitoring. Dissolved oxygen ph Temperature Offgas monitoring Substrate (glucose)
Bioreactor Monitoring & Control Bioreactor Monitoring & Control Basic principles of process control Fermentation monitoring Dissolved oxygen ph Temperature Offgas monitoring Substrate (glucose) Useful
More informationThe scale-up of microbial batch and fed-batch fermentation processes
Loughborough University Institutional Repository The scale-up of microbial batch and fed-batch fermentation processes This item was submitted to Loughborough University's Institutional Repository by the/an
More informationImplementation of a Micro Bioreactor System for Platform Cell Culture Process Development at Cobra Biologics
Implementation of a Micro Bioreactor System for Platform Cell Culture Process Development at Cobra Biologics Kristina Lae, Scientist, Cell Culture Cobra Biologics, Södertälje, Sweden Cobra Biologics and
More informationViresolve Pro Solution
Data Sheet Viresolve Pro Solution Proven parvovirus safety solution designed to provide the highest levels of retention assurance and productivity Robust. Productive. Proven. The Solution provides a comprehensive,
More information2008 International ANSYS Conference
2008 International ANSYS Conference Computational Modeling of Industrial Biofuel Reactors Jaydeep Kulkarni Presenter: Genong Li Technical Account Manager ANSYS, Inc. 2008 ANSYS, Inc. All rights reserved.
More informationSINGLE & PARALLEL FERMENTERS/ BIOREACTORS
SINGLE & PARALLEL FERMENTERS/ BIOREACTORS simply represents the next generation of parallel autoclavable R&D fermenters/bioreactors with a prepacked high tech innovative solutions, ready out of the box
More informationA Case Study of the Design Calculation for a Logical Biogas Plant
Appendix I A Case Study of the Design Calculation for a Logical Biogas Plant As already mentioned, the Sugar Cane & Byproducts Development Company produces about 7.6 million tonnes sugar cane and 2.4 million
More informationF-SOP-Z-3.7 WZÓR ZAPYTANIA OFERTOWEGO OBOWIĄZUJE W BLIRT S.A. Wersja 1 Ob. od
Gdansk, 24.03.2017 Request for Proposal No. 10/2017/KT Subject: Bioreactor System for microbial fermentation with working volume of a 400 liters training and installation CPV CODE: 38500000-0 NAME AND
More informationThe Advantages of Automatic Quality Control (AQC) for Blood Gas Testing QC
Siemens Healthcare Diagnostics, the leading clinical diagnostics company, is committed to providing clinicians with the vital information they need for the accurate diagnosis, treatment and monitoring
More informationF-SOP-Z-3.7 WZÓR ZAPYTANIA OFERTOWEGO OBOWIĄZUJE W BLIRT S.A. Wersja 1 Ob. od
Gdansk, 7.04.2017 Request for Proposal No. 11/2017/KT Subject: Bioreactor System for microbial fermentation with working volume of a 400 liters with training and installation CPV CODE: 38500000-0 NAME
More informationEFFECT OF SOLIDS CONCENTRATION ON SOLID-LIQUID MASS TRANSFER IN AN AGITATED DISSOLUTION SYSTEM
EFFECT OF SOLIDS CONCENTRATION ON SOLID-LIQUID MASS TRANSFER IN AN AGITATED DISSOLUTION SYSTEM Chongguang Yu¹, Rajarathinam Parthasarathy 1 *, Jie Wu 2 and Dr. Nicky Eshtiaghi 1 1 School of Civil, Environmental
More informationi-control Complete Process Control
icontrol icontrol TOTAL TOTAL CONTROL CONTROL MAKE MAKE SELECTION SELECTION SELECT CONTROL OPTION FOR I-CONTROL i-control Complete Process Control Applikon Biotechnology T H E B I O P R O C E S S I N G
More informationBio Reactor Systems. Contact for further information, or call +44 (0)
Bio Reactor Systems The bench mark for R+D Bio-reactors Single and parallel fully automated, modular systems Aerobic, anaerobic plus microbial, cell cultures and bio-fuels Range of interchangeable size
More informationSingle-use bioreactor for process intensification
USD2941 (1) icellis Bioreactor Single-use bioreactor for process intensification Highlights and Benefits Process simplification, from pre-culture to final product The first fully-integrated, single-use
More informationCFD MODELLING OF A NOVEL STIRRED REACTOR FOR THE BIO- PRODUCTION OF HYDROGEN
14 th European Conference on Mixing Warszawa, 1-13 September 212 CFD MODELLING OF A NOVEL STIRRED REACTOR FOR THE BIO- PRODUCTION OF HYDROGEN G. Montante a, M. Coroneo a, J.A. Francesconi b, A. Paglianti
More informationCLOSED-LOOP HEAT EXCHANGER FOR GROUND COUPLED HEAT PUMPS
Bulletin of the Transilvania University of Braşov Vol. () - 0 Series : Special Issue No. CLOSED-LOOP HEAT EXCHANGER FOR GROUND COUPLED HEAT PUMPS G. DRAGOMIR I. BOIAN V. CIOFOAIA Abstract: Hydraulic imbalances
More informationCultivation of sensitive cell lines - Improving bioreactor performance by dynamic membrane aeration
Cultivation of sensitive cell lines - Improving bioreactor performance by dynamic membrane aeration Björn Frahm, Helmut Brod Bioprocessing Summit Optimizing Cell Culture Technology, Boston, 2010-08-24
More informationInline Conditioning system
GE Healthcare Frequently asked questions Inline Conditioning system Buffer preparation can be both time-and spaceconsuming and can easily become a challenge in biomanufacturing. In-line preparation of
More informationE APPENDIX. The following problems are intended for solution using finite element. Problems for Computer Solution E.1 CHAPTER 3
E APPENDIX Problems for Computer Solution The following problems are intended for solution using finite element analysis software. In general, the problems associated with Chapters 3, 4, and 9 can be solved
More informationSOP: Applikon ez-control Bioreactor Controller Operation
Page 1 of 19 Approvals Preparer: John Buford Reviewer: Jason McMillan Reviewer: Dr. Margaret Bryans Date: 19DEC13 Date: 20DEC13 Date: 21DEC13 1. Purpose 1.1. Cultivate a cell culture using an Applikon
More informationCharacterization of agitation environments in 250 ml spinner vessel, 3 L, and 20 L reactor vessels used for animal cell microcarrier culture
Cytotechnology 22: 95??, 1996. 95 c 1996 Kluwer Academic Publishers. Printed in the Netherlands. Special Issue Characterization of agitation environments in 250 ml spinner vessel, 3 L, and 20 L reactor
More informationBioreactor System ERT 314. Sidang /2011
Bioreactor System ERT 314 Sidang 1 2010/2011 Chapter 2:Types of Bioreactors Week 2 Choosing the Cultivation Method The Choice of Bioreactor Affects Many Aspects of Bioprocessing. Product concentration
More informationOXIDATIVE PRECIPITATION OF Fe AND Mn BY AIR/SO 2
OXIDATIVE PRECIPITATION OF Fe AND Mn BY AIR/SO 2 Michelle Mouton a, Johanna van Deventer a and Jussi Vaarno b a. Mintek, South Africa b. Outotec, Finland Mintek has been involved in numerous projects to
More informationField and CFD Analysis of Jet Aeration and Mixing
Field and CFD Analysis of Jet Aeration and Mixing Randal W. Samstag 1 *, Edward A. Wicklein 1, Roderick D. Reardon 2, Robert J. Leetch 3, R. (Robbie) M. Parks 3, and Colin D. Groff 3 1 Carollo Engineers,
More informationHydrodynamic and Mass Transfer Study in a Mechanically Stirred Hybrid Airlift Bioreactor Based on Impeller Type
Hydrodynamic and Mass Transfer Study in a Mechanically Stirred Hybrid Bioreactor Based on Impeller Type Sérgio S. de Jesus, Aline Santana, and Rubens Maciel Filho Abstract The analysis of the influence
More information3/5/2014. Key Topics for Consideration
Hole Cleaning in Deviated Wellbores Terry Hemphill Global Advisor, Wellbore Management Halliburton-Baroid Houston, Texas AADE Fluids Management Group 27 February 2014 terry.hemphill@halliburton.com Key
More informationApplication Note USD Pall SoloHill Animal Protein-Free Microcarriers for Dengue Virus Production
Application Note USD 8 Pall SoloHill Animal Protein-Free Microcarriers for Dengue Virus Production Introduction Microcarrier cultures have a long and successful record for use in the large-scale production
More informationYour Guide to Upstream Processing Solutions From Research to Production
Your Guide to Upstream Processing Solutions From Research to Production Upstream Process Overview Our Solutions for Your Upstream Processing Needs Sartorius offers a wide range of process solutions for
More informationHomework #6. From the primary textbook (Shuler, et. al. 3 rd ed.), problems 10.2, 10.5, 10.8.
Homework #6 From the primary textbook (Shuler, et. al. 3 rd ed.), problems 10.2, 10.5, 10.8. CBEN 460 Fall 2017 1 October 18, 2017 AMANULLAH, A., J. M. 0TERRO, M. MIKOLA, A. Hsu, J. ZHANG, J. AUNINS, H.
More informationOne-step seed culture expansion from one vial of high-density cell bank to 2000 L production bioreactor
GE Healthcare One-step seed culture expansion from one vial of high-density cell bank to 2 L production bioreactor This application note describes how perfusion cell culturing can be used to reduce processing
More informationFLOW MEASUREMENT Product Code 316 Instruction manual Contents
FLOW MEASUREMENT Product Code 316 Instruction manual Contents 1 Description 2 Specifications 3 Installation requirements 4 Installation Commissioning 5 Troubleshooting 6 Components used 7 Packing slip
More informationHollow Fiber Bioreactors: Single-Use. Perfusion. Scalable. Continuous Manufacturing.
Bioreactors and BioServices for the Life of your Proteins Hollow Fiber Bioreactors: Single-Use. Perfusion. Scalable. Continuous Manufacturing. Presented by: Scott Waniger Vice President, BioServices BPI
More informationPREPARED BY: DR. RAHIMAH OTHMAN FOOD ENGINEERING (ERT 426) SEMESTER 1 ACADEMIC SESSION 2016/17
1 PREPARED BY: DR. RAHIMAH OTHMAN FOOD ENGINEERING (ERT 426) SEMESTER 1 ACADEMIC SESSION 2016/17 SUBTOPICS 2 1. Introduction 2. Basic Principles of Extrusion 3. Extrusion System 3.1 Cold Extrusion 3.2
More informationThe Use of Disposable Technologies in Antibody Manufacturing Processes
The Use of Disposable Technologies in Antibody Manufacturing Processes Tim Matthews and Brad Wolk Process Development Engineering Genentech, Inc. South San Francisco, CA Outline Rationale for using disposable
More informationnhc EARTH TECH CANADA INC. CITY OF WINNIPEG NORTH END WATER POLLUTION CONTROL CENTRE PUMP STATION MODEL TEST FINAL REPORT JANUARY 2005
EARTH TECH CANADA INC. CITY OF WINNIPEG NORTH END WATER POLLUTION CONTROL CENTRE PUMP STATION MODEL TEST FINAL REPORT JANUARY 2005 nhc northwest hydraulic consultants CITY OF WINNIPEG NORTH END WATER POLLUTION
More informationBasic design of laboratory bioreactor
Basic design of laboratory bioreactor In terms of the construction, the following variants of the laboratory bioreactor can be made: 1. Glass bioreactor (without a jacket) with an upper stainless steel
More informationDavid Baker Piping Technology & Products. NIA s 62nd Annual Convention Sheraton Grand at Wild Horse Pass March 29 April 1, 2017
David Baker Piping Technology & Products NIA s 62nd Annual Convention Sheraton Grand at Wild Horse Pass March 29 April 1, 2017 Piping Technology & Products, Inc. Piping Technology & Products, Inc. (PT&P)
More informationMAX300-BIO PRODUCT NOTE. Bioreactor/Fermentation Control Process Efficiency Product Quality Analysis. Bioreactor/Fermentation Mass Spectrometer
MAX300-BIO Bioreactor/Fermentation Mass Spectrometer www.extrel.com PRODUCT NOTE Bioreactor/Fermentation Control Process Efficiency Product Quality Analysis Introducing the MAX300-BIO Know Your Process
More informationHYDRAULIC DEMONSTRATION CHANNEL
HYDRAULIC DEMONSTRATION CHANNEL PACKING LIST Pitot-Static Tube and Manometer Tube Flow Meters 1. 48 Long Differential Manometer 2. Clear Tubing 3. Pitot-static Tube Clamp 4. Pitot-static Tube 1. Venturi
More informationStatic Mixing, Reaction, Heat Transfer & Fluid Dynamics Technology
stamixco Static Mixing, Reaction, Heat Transfer & Fluid Dynamics Technology equipment with no moving parts for the continuous processing of polymers, liquids gases and solids General Brochure Note: The
More informationProduct Bulletin 2K-3.2. stamixco. mix it up..
stamixco Product Bulletin 2K-3.2 mix it up.. 2-Component Resin Mixing Technology Plastic Disposable & Metal Static Mixers for Mixing Viscous Materials 2 Introduction For more than 40 years, static mixers
More informationPreliminary Studies on In Situ Monitoring of Lactose Crystallization Using Focused Beam Reflectance Measurement
Dairy Research 214 Preliminary Studies on In Situ Monitoring of Lactose Crystallization Using Focused Beam Reflectance Measurement K. Pandalaneni, J.K. Amamcharla Summary Isothermal crystallization of
More informationDriving Innovation Through Bioengineering Solutions. a world-class business in a global hub for biotechnology
Driving Innovation Through Bioengineering Solutions a world-class business in a global hub for biotechnology Process Scale-Up & Tech Transfer Capabilities Unique blend of engineering and biotechnology
More informationGilflo flowmeters. for steam, liquids and gases
Gilflo flowmeters for steam, liquids and gases The Gilflo spring loaded variable area flowmeter... offers an unrivalled 1:1 turndown and can be used to meter most industrial fluids including steam and
More informationMeasurement of Two Phase Flows in Geothermal Pipelines Using Load-Cells: Field Trial Results
PROCEEDINGS, 41st Workshop on Geothermal Reservoir Engineering Stanford University, Stanford, California, February 22-24, 2016 SGP-TR-209 Measurement of Two Phase Flows in Geothermal Pipelines Using Load-Cells:
More informationA CFD ANALYSIS OF CENTRIFUGAL PUMP TO IMPROVE DISCHARGE BY VARYING BLADE GEOMETRY
A CFD ANALYSIS OF CENTRIFUGAL PUMP TO IMPROVE DISCHARGE BY VARYING BLADE GEOMETRY 1 ANJANI KUMAR SINHA, 2 A. JOHN RAJAN, 3 ERIKI ANANDA KUMAR 1,3 Senior Lecturer, Dept. of Mech. Eng., Nilai University,
More information[4163] T.E. (Mechanical) TURBO MACHINES (2008 Pattern) (Common to Mech. S/W) (Sem. - II)
Total No. of Questions : 12] P1061 SEAT No. : [Total No. of Pages : 7 [4163] - 218 T.E. (Mechanical) TURBO MACHINES (2008 Pattern) (Common to Mech. S/W) (Sem. - II) Time : 3 Hours] [Max. Marks :100 Instructions
More informationAeration Systems Past, Present and Future. What to Expect from Aeration System Upgrades
Aeration Systems Past, Present and Future. What to Expect from Aeration System Upgrades Michael K. Stenstrom Professor, Civil and Environmental Engineering Department Outline Aeration system types Terminology
More informationWastewater Treatment Plant Energy Reduction With High Efficiency Aerators
Wastewater Treatment Plant Energy Reduction With High Efficiency Aerators Copyright 2011, Saudi Aramco. All rights reserved. Rabea Manakhah, David Evans Saudi Aramco Utilities Department Roger Gyger m2t
More informationMEASUREMENT OF CAVERN SIZES AND SHAPE IN AGITATED YIELD STRESS AQUEOUS SOLUTIONS WITH AN ELECTROCHEMICAL PROBE
14 th European Conference on Mixing Warszawa, 1-13 September 212 MEASUREMENT OF CAVERN SIZES AND SHAPE IN AGITATED YIELD STRESS AQUEOUS SOLUTIONS WITH AN ELECTROCHEMICAL PROBE Takaya Nagafune a, Yushi
More informationXpansion Multiplate Bioreactor System
USD939 (1) Xpansion Multiplate Bioreactor System Multiplate bioreactor for cell-based product manufacturing Features and Benefits USP Class VI certified materials Designed for adherent cell culture applications
More informationHydro Electric Power (Hydel Power)
Operating Principle Hydro Electric Power (Hydel Power) Hydro-electric power is generated by the flow of water through turbine, turning the blades of the turbine. A generator shaft connected to this turbine
More informationAgitator Design Principles for Bioprocessing and Pharmaceutical Applications
Agitator Design Principles for Bioprocessing and Pharmaceutical Applications Who Should Attend This is a course for people who must specify, operate, purchase or optimize fluid agitation equipment used
More informationEFFECT OF BAFFLES ON SOLID-LIQUID MASS TRANSFER COEFFICIENT IN HIGH SOLID CONCENTRATION MIXING
EFFECT OF BAFFLES ON SOLID-LIQUID MASS TRANSFER COEFFICIENT IN HIGH SOLID CONCENTRATION MIXING Eng Ying Bong 1, Rajarathinam Parthasarathy 1 *, Jie Wu 2 and Nicky Eshtiaghi 1 1 School of Civil, Environmental
More informationCalculate the Costs of Piping System Elements
Calculate the Costs of Piping System Elements by Ray Hardee, Engineered Software, Inc. Last month s column described the process of creating an energy cost balance sheet for a piping system (see Figure
More information