All-In-One for cell research

Transcription

1 Live cell movie analyzer All-In-One for cell research Crazy + Tangible play, 2K13

2 What is JuLI TM Br counting? JuLI Br TM Counting software is designed to measure cell count and viability (live, dead, and total cells) accurately and precisely, using the standard trypan blue staining through the innovative Semi-Auto focus technology.

3 Key features Revolutionary counting accuracy Provides accurate cell counts with advanced counting algorithm in clumped cell Fast counting time Counts all type of the cell lines less than 10 seconds Innovative Semi-Auto focusing technology Reproducible and reliable counting results without variation of manual focusing Applicable for broad range of cell sizes and types Primary (tissue, blood) cell lines and stem cells User friendly interface 10.1 inch color LCD touch screen, Focus adjustment through LCD No maintenance needed Cell size gating Select range of cell size using gating function

4 Software description Scope unit channel Cell size graph Zoom in/out Cell counting result Exposure bar Brightness bar Focus interface Semi-auto focusing button Setting bar of min. & max. cell size Save to USB button Cell counting button

5 Counting procedures Load the samples Adjust the focus Obtain the results

6 Correlation of JuLI TM Br and manual counting 1.0E E+06 JuLI TM Br Hemocytometer 6.0E E E E E E E E E E+07 Measuring from the JuLI Br extend further along the high concentration range than hemocytometer readings.

7 Total count (cells/ml) Total count (cells/ml) Total cell counting results Non-clumped cell Clumped cell 2.5E E+06 JuLI TM Br Hemocytometer JuLI BR Competitor s Hemocytometer automated cell counter TC20 2.5E E+06 JuLI JuLI TM BR Br Hemocytometer Competitor s Hemocytometer automated cell counter TC20 1.5E E E E E E E E+00 HeLa U-2 os Jurkat NIH-3T3 HepG2 MCF7 F9 Non-clumped cells (HeLa, U-2 os, Jurkat, NIH-3T3), and clumped cell (HepG2, MCF7, F9) cells were counted with JuLI TM Br, a hemocytometer, a competitor s automated cell counter. Accuracy and precision are comparable between the JuLI TM Br, a hemocytometer, a competitor s automated cell counter for all cell lines. The competitor s automated cell counter is significantly less accurate in clumped cell counting. Cell counting results were performed on three different counters with seven sample replicates.

8 Cell Viability (%) Cell Viability (%) Viability Non-clumped cell Clumped cell JuLI TM Br Hemocytometer Competitor s automated cell counter JuLI TM Br Hemocytometer Competitor s automated cell counter HeLa U-2 os Jurkat NIH-3T3 HepG2 MCF7 F9 Non-clumped cells (HeLa, U-2 os, Jurkat, NIH-3T3), and clumped cell (HepG2, MCF7, F9) cells were counted with a JuLI TM Br, a hemocytometer and the competitor s automated cell counter. Accuracy and precision are comparable between the JuLI TM Br and the hemocytometer for all cell lines. The competitor s automated cell counter is significantly less accurate in clumped cell counting. Cell counting results were performed on three different instruments with seven sample replicates.

9 MCF7 Comparison image (Clumped cells) JuLI TM Br Competitor s automated cell counter Clumped cell (Not detected) Clumped cell (Detecting individual cells) Live cell Dead cell Live cell Dead cell The MCF7, breast cancer cell line can be clumpy. The JuLI TM Br counting algorithm identifies and counts individual cells within these cell clumps for accurate analysis.

10 F9 Comparison image (Clumped cells) JuLI TM Br Competitor s automated cell counter Clumped cell Clumped cell Live cell Dead cell Live cell Dead cell The F9, breast cancer cell line can be clumpy. The JuLI TM Br counting algorithm identifies and counts individual cells within these cell clumps for accurate analysis.

11 Semi-Auto focusing Semi-Auto focus function to get more accurate focus without individual variation of manual focusing. Focus by users in various cases APPLY Semi-Auto focusing Total counts Viability Total counts Viability Total counts Viability Before A/F 2.6 X 10E % 2.4 X 10E % 2.5 X 10E % After A/F 2.5 X 10E % 2.5 X 10E % 2.5 X 10E %

12 Analysis Time (Seconds) Analysis Time (Seconds) Fast counting time Non-clumped cell Clumped cell 50.0 NIH-3T HepG Jurkat 40.0 MCF HeLa U-2 os 30.0 F JuLI BR Competitor s TC20 Countess automated cell counter 0.0 JuLI BR Competitor s TC20 Countess automated cell counter The JuLI Br has most advanced cell counting algorithm to reduce the cell counting time and the JuLI Br is about 2 ~ 3 times faster than competitor s automated cell counter. (Cell concentration: 2ⅹ10 6 cells/ml)

13 Cell lines validated on JuLI TM Br Cell Type Animal Organ Growth Properties HeLa Human Skin Adherent NIH-3T3 Mouse Embryo Adherent U2-OS Human Bone Adherent Jurkat Human Blood Suspension KG-1 Human Blood Suspension HepG2 Human Liver Adherent Hep3B Human Liver Adherent LNCaP Human Prostate Adherent SH-SY5Y Human Brain Adherent SCN2.2 Rat Brain Adherent F9 Mouse Embryo Adherent MCF7 Human Breast Adherent A549 Human Lung Adherent GH3 Rat Pituitary gland Adherent

14 Specifications JuLI TM Br Counting SW Counting time < 10 seconds Cell measurement range (cells/ml) 1 x x 10 7 Optimal measurement range (cells/ml) 1 X X 10 6 Cell size range 5 60 µm Sample volume 10 µl Staining method Display Image format Data export Trypan blue stain 10.1 LCD color touch screen JPEG (image), CSV (raw data) USB drive JuLI TM Br Cell counting slide Material Dimensions Polymethyl methacrylate 75 mm (L) 25 mm (W) 1.8 mm (H) Chamber depth 100 µm Chamber volume 10 µl

15 Thank You. Crazy + Tangible play, 2K13 Copyright NanoEnTek Inc. All rights reserved ver. 0.0 Website sales@nanoentek.com NanoEnTek Inc. 12F, Ace High-end Tower, 235-2, Guro3-dong, Guro-gu, Seoul, , Korea Tel : Fax : NanoEnTek USA Inc Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA Tel : , (Toll free) Fax :