Aldehyde Arraying Slides High quality slides designed for array production
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1 Protocol Aldehyde Arraying Slides High quality slides designed for array production Control #: 03PCT1001.A0 Effective Date: 01-Jul-11 ECO #: 4002
2 Content Page Introduction 3 Technical Information 3 Protocol 4 Storage Store at room temperature For Research Use Only Molecular Devices > 2 of 5
3 Introduction Molecular Devices Aldehyde slides are high quality slides designed for array production. The slides covalently bind your target to the glass surface via the Schiff base aldehydeamine chemistry (for example lysine residues of proteins and primary amines of DNA bases) Technical Information Suggested spotting concentrations for your targets are µg/µl Molecular Devices Aldehyde coated slides are not compatible for spotting samples in DMSO, or Molecular Devices Amine Spotting Solution. Cat # Description K2625 Aldehyde Coated Slides Quantity 25 K2630 Aldehyde Coated Slides Quantity 100 K2630C Aldehyde Coated Slides (Clear slides with no frosting) Quantity 100 Molecular Devices > 3 of 5
4 Protocol Array your targets onto the slides using Molecular Devices Aldehyde Microarray Spotting Solution (Cat # K2055 / K2060 / K2065), or another appropriate spotting solution, such as 3XSSC). Leave the slides for a minimum of 4 hours in a constant humidity environment. Process the slides as follows: Wash slide twice in 0.2% SDS at room temperature for 2 minutes. Wash slide twice in dh 2 0 at room temperature for 2 minutes. Wash in dh 2 0 at 95 C C for 2 minutes. Allow to cool for 30 seconds. Wash for 5 minutes in Sodium borohydride mix at room temperature (1g Sodium borohydride dissolved in 300ml 1xPBS and 100ml 100% ethanol). Wash 3 times in 0.2% SDS at room temperature for 1 minute. Rinse twice in dh 2 0 for 2-3 seconds at room temperature. Dry the slides by placing in a slide dryer (Cat # X2510) and centrifuging for 1 minute at 100 x g (1000rpm) for 1 minute. Store slides in the dark at room temperature. At this point the processed slides can be stored for several months. To hybridize your slide, react the slide with a labelled probe in genhyb microarray hybridization buffer (Cat # K2102 / K2105), using a hybridisation chamber (Cat # K2236) or similar sealed humid chamber. Hybridise the slide according to protocol for the hybridisation buffer. Transfer the slide to a Slide Washer (Cat # K2239) and wash according to the genhyb hybridization protocol. Place the slide in a slide dryer (Cat # X2510) and centrifuge at 100 x g (1000 rpm) for 1 minute. Visualise the array by scanning with an appropriate fluorochrome channel on a scanner. Molecular Devices > 4 of 5
5 Contact Details Molecular Devices Queensway, New Milton Hampshire BH25 5NN, UK Tel: +44 (0) Fax: +44 (0) Web: For all technical queries please contact your nearest Customer Support group. Visit for latest contact details. Trademarks ClonePix, CloneSelect, CellReporter, HalfBD, 'NRich, SlidePath, Data Arena and Image Arena are trademarks of Molecular Devices (New Milton) Ltd. Copyright 2011 by Molecular Devices (New Milton) Ltd All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form by any means, electronic, mechanical, by photocopying, recording, or otherwise, without the prior written permission of Molecular Devices (New Milton) Ltd. Information furnished by Molecular Devices (New Milton) Ltd is believed to be accurate and reliable; however, no responsibility is assumed by Molecular Devices (New Milton) Ltd, for its use; nor for any infringements of patents or other rights of third parties which may result from its use. No license is granted by implication or otherwise under any patent rights of Molecular Devices (New Milton) Ltd. Revised July 2011 Molecular Devices > 5 of 5
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Protocol. NEXTERION Slide H
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Protocol. NEXTERION Slide H
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Protocol. Nexterion Slide H
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