Content. Gold Nanoparticles 1. Quantum Dots 3. Bionanoconjugates 5. Microbial Surfactants 8. Custom Assay Development Service 10

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1 Content Products Page Gold Nanoparticles 1 Quantum Dots 3 Bionanoconjugates 5 Microbial Surfactants 8 Services Custom Assay Development Service 10 Custom Bioconjugation Services 11 Custom Cloning Service 12 Microbial Identification Service 13 Protein Purification Service

2 Gold Nanoparticles Gold has always been the one precious material people like best. One of these special uses of gold refers to what is called nanogold. colloidal gold or gold nanoparticles, i.e. sub-micrometer sized particles of gold dispersed in a fluid, usually water. Gold Nanoparticles have unique physical, chemical and biological properties such as inertia, stability, biocompatibility, low level of cytoxicity and others, cause significant medico-biological potential of gold nanoparticles and utilized in high end applications such as organic photovoltaics, sensory probes, therapeutics, drug delivery, electron microscopy, electronic conductors and catalysis. The optical and electronic properties of gold nanoparticles are tunable by changing the size, shape, surface chemistry or aggregation state. This leads to the search of new and more advanced method of controlled synthesis of gold nanoparticles and henceforth, controlled property of size and shape. The broad range of gold nanoparticles by Apollo Biolife, serves to increase their adoption in high-technology applications giving optimum performance, with superior stability, sensitivity and binding properties. Non-functionalized Gold Nanoparticles Our Standard spherical gold nanoparticles are prepared using modified proprietary citrate reduction method. Product ID Product Name Pack Size Gold Nanoparticles- 2 nm 50 ml Gold Nanoparticles- 2 nm 200 ml Gold Nanoparticles- 5 nm 50 ml Gold Nanoparticles- 5 nm 200 ml Gold Nanoparticles- 10 nm 50 ml Gold Nanoparticles- 10 nm 200 ml 1 P a g e

3 Product ID Product Name Pack Size Gold Nanoparticles- 20 nm 50 ml Gold Nanoparticles- 20 nm 200 ml Gold Nanoparticles- 30 nm 50 ml Gold Nanoparticles- 30 nm 200 ml Gold Nanoparticles- 40 nm 50 ml Gold Nanoparticles- 40 nm 200 ml Gold Nanoparticles- 60 nm 50 ml Gold Nanoparticles- 60 nm 200 ml Functional Gold Nanoparticles Product ID Product Name Pack Size 2246A1 Amine Terminal Gold Nanoparticles- 30 nm 50 ml 2246A2 Amine Terminal Gold Nanoparticles- 40 nm 50 ml 2246C1 Carboxy Terminal Gold Nanoparticles- 30 nm 50 ml 2246C2 Carboxy Terminal Nanoparticles- 40 nm 50 ml 2 P a g e

4 Quantum Dots Quantum dots are tiny particles, or nanoparticles, of a semiconductor material which range from 2 to 10 nanometers in diameter (about the width of 50 atoms). Fundamentally, quantum dots are fluorophores substances that absorb photons of light, then re-emit photons at a different wavelength. The wavelength of these photon emissions is dependent upon the size of the quantum dot. Quantum dots are extremely efficient in generating fluorescence; their intrinsic brightness is often many times that observed for other classes of fluorophores. The photo-stability of a quantum dot is many orders of magnitude greater than that associated with traditional fluorescent molecules; a property which enables them to have long-term imaging experiments which is difficult to achieve with conventional fluorophores that show photo-induced deterioration. The ability to precisely control the size of a quantum dot enables the manufacturer to determine the wavelength of the emission, which in turn determines the colour of light the human eye perceives. Quantum dots can therefore be tuned during production to emit any colour of light desired. The ability to control, or tune the emission from the quantum dot by changing its core size is called the size quantization effect. The smaller the dot, the closer it is to the blue end of the spectrum, and the larger the dot, the closer to the red end. Dots can even be tuned beyond visible light, into the infra-red or into the ultra-violet. Our range of semi-conductor fluorescent nanoparticles finds their use in numerous applications in the field of nanoelectronics and opto-electronics. They are offered in both organic and aqueous formulations. Our research and development department is also constantly engaged in the creation of new quantum dots with additional properties sought by the market. 3 P a g e

5 Product ID Product Name Pack Size Amine Functionalized Quantum Dots 10 mg/ml in H 2 O (em 510 nm ) Amine Functionalized Quantum Dots 10 mg/ml in H 2 O (em 530 nm ) Amine Functionalized Quantum Dots 10 mg/ml in H 2 O (em 555 nm ) Amine Functionalized Quantum Dots 10 mg/ml in H 2 O (em 570 nm ) Amine Functionalized Quantum Dots 10 mg/ml in H 2 O (em 590 nm ) Amine Functionalized Quantum Dots 10 mg/ml in H 2 O (em 610 nm ) Carboxylate Functionalized Quantum Dots 10 mg/ml in H 2 O (em 510 nm ) Carboxyl Acid Functionalized Quantum Dots 10 mg/ml in H 2 O (em 530 nm ) Carboxyl Acid Functionalized Quantum Dots 10 mg/ml in H 2 O (em 555 nm ) Carboxyl Acid Functionalized Quantum Dots 10 mg/ml in H 2 O (em 570 nm ) Carboxyl Acid Functionalized Quantum Dots 10 mg/ml in H 2 O (em 590 nm ) Carboxyl Acid Functionalized Quantum Dots 10 mg/ml in H 2 O (em 610 nm ) Quantum Dots with alkyl ligand surface 10 mg/ml in Toluene (em 450 nm ) Quantum Dots with alkyl ligand surface 10 mg/ml in Toluene (em 490 nm ) Quantum Dots with alkyl ligand surface 10 mg/ml in Toluene (em 525 nm ) Quantum Dots with alkyl ligand surface 10 mg/ml in Toluene (em 575 nm ) Quantum Dots with alkyl ligand surface 10 mg/ml in Toluene- (em 630 nm ) Quantum Dots with alkyl ligand surface 10 mg/ml in Toluene- (em 665 nm ) 4 P a g e

6 Bionanoconjugates Apollo Biolife offers high quality research grade nanoparticles-proteins bionanoconjugates which are manufactured to the highest specifications resulting in low size coefficient of variation and excellent sensitivity. These bionanoconjugates find exceptional use in applications such as western blot, dot-blot, lateral flow assays, fluorescence assays, flow cytometry, TEM, in vivo and in vitro imaging etc. Product ID Product Name Pack Size Anti-Human Antibody- 10 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti-Human Antibody- 20 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti-Human Antibody- 30 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti-Human Antibody- 40 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti- Mouse Antibody- 10 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti- Mouse Antibody- 20 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti- Mouse Antibody- 30 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti- Mouse Antibody -40 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti- Rabbit Antibody- 10 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti- Rabbit Antibody- 20 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti- Rabbit Antibody- 30 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti- Rabbit Antibody -40 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Protein A - 10 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Protein A - 20 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Protein A - 30 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml 5 P a g e

7 Product ID Product Name Pack Size Protein A - 40 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Streptavidin - 10 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Streptavidin - 20 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Streptavidin - 30 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Streptavidin - 40 nm Gold Nanoparticles Conjugate (O.D 20) 1 ml Anti-Human Antibody- Quantum Dots conjugate (em 510 nm ) Anti-Human Antibody- Quantum Dots conjugate (em 530 nm ) Anti-Human Antibody- Quantum Dots conjugate (em 555 nm ) Anti-Human Antibody- Quantum Dots conjugate (em 570 nm ) Anti-Human Antibody- Quantum Dots conjugate (em 590 nm ) Anti-Mouse Antibody- Quantum Dots conjugate (em 510 nm ) Anti-Mouse Antibody- Quantum Dots conjugate (em 530 nm ) Anti-Mouse Antibody- Quantum Dots conjugate (em 555 nm ) Anti-Mouse Antibody- Quantum Dots conjugate (em 570 nm ) Anti-Mouse Antibody- Quantum Dots conjugate (em 590 nm ) Anti-Rabbit Antibody- Quantum Dots conjugate (em 510 nm ) Anti-Rabbit Antibody- Quantum Dots conjugate (em 530 nm ) Anti-Rabbit Antibody- Quantum Dots conjugate (em 555 nm ) Anti-Rabbit Antibody- Quantum Dots conjugate (em 570 nm ) Anti-Rabbit Antibody- Quantum Dots conjugate (em 590 nm ) Protein A- Quantum Dots conjugate (em 510 nm ) Protein A- Quantum Dots conjugate (em 530 nm ) Protein A- Quantum Dots conjugate (em 555 nm ) 0. 6 P a g e

8 Product ID Product Name Pack Size Protein A- Quantum Dots conjugate (em 570 nm ) Protein A- Quantum Dots conjugate (em 590 nm ) Streptavidin- Quantum Dots conjugate (em 510 nm ) Streptavidin- Quantum Dots conjugate (em 530 nm ) Streptavidin- Quantum Dots conjugate (em 555 nm ) Streptavidin- Quantum Dots conjugate (em 570 nm ) Streptavidin- Quantum Dots conjugate (em 590 nm ) 0. 7 P a g e

9 Microbial Surfactants Surfactants are chemicals that reduce surface tension (or interfacial tension) between two liquids or between liquid and solid. Surfactants find wide application which range from simple household activities to complicated industrial processes. Surfactants are widely used in soaps, laundry detergent, dish washing liquids and personal care products such as moistures, shampoos. They are also extensively used in lubricants, emulsion polymerization, textile processing, mining flocculates, petroleum recovery, and wastewater treatment. Apollo Biolife has developed bio-based surfactants that are environment friendly and less toxic than conventional synthetic surfactants. Apollo biolife make its biosurfactant using simple fermentation technology. Currently, Apollo biolife has successfully produced rhamnolipids and surfactin biosurfactants, further making it commercially available and economical for the first time in India. Research and development section, Apollo Biolife is also currently working on other well known biosurfactants like Sophorolipids Iturin, Fengycin, Lichenysin and Bacillomycin. Products with biosurfactant provide good emulsification, wetting, detergency, and foaming properties, along with very low toxicity. They are readily biodegradable and leave no harmful or persistent degradation products. Their superior qualities make them suitable for many diverse applications. Rhamnolipids is a well studied glycolipids type biosurfactant. Apollo Biolife produces this biosurfactant commercially in controlled, aerobic fermentations using high yielding strains of Pseudomonas aeruginosa. Efficient downstream processing makes it suitable for pharmaceutical and cosmeceuticals applications. Rhamnolipids are also suitable for environmental remediation of hydrocarbons, heavy metals and pesticides in soil and water. Surfactin is a lipopeptide type biosurfactant with renowned antimicrobial activity. Apollo Biolife produces this biosurfactant for commercial and research purpose in aerobic fermentations using high yielding strains of Bacillus subtilis. 8 P a g e

10 Product ID Product Name Pack Size Rhamnolipids 10mg Mono-Rhamnolipids 10mg Di-Rhamnolpids 10mg Aqueous Rhamnolipids solution (1mg/ml) 10ml Surfactin 10mg Aqueous Surfactin Solution (1mg/l) 10ml 9 P a g e

11 Custom Assay Development Service Apollo Biolife is proud to be the first and as of today, only company from India to offer custom ELISA and Dipstick/Lateral Flow Assay Development service. When you choose to outsource your assay development to us, you can be sure that we will work with you through every step of the development process. Our assign design process ensures a robust assay which ensures robust manufacture. Characteristics of Apollo Biolife Assay Development Service: Assay formats Applications Single or multi analyte Veterinary Antibody or antigen sandwich Environmental testing Competitive Infectious disease Qualitative Military Semi-quantitative Food safety Quantitative tests / readers. Clinical Sample Matrices Labels Whole blood, serum or plasma Gold colloid Urine Latex Saliva Molecular Food and milk Enzymes Culture media. Salient Features Flexible Cost Effective Fully Phased Order Information Custom Assay Development Service 10 P a g e

12 Custom Bioconjugation Services Protein- Nanoparticles Conjugation Apollo Biolife provides custom conjugation of your antibodies or other proteins to our gold nanoparticles and quantum dots. If you want us to source your protein of antibody we would be glad to assist Our services include: 1. Sourcing of your antibody or protein if needed (this service is not included in price). 2. Conjugation of your protein to a nanoparticle of your choice. 3. Purification of conjugate. Oligonucleotides Nanoparticles Conjugation Apollo Biolife provides custom conjugation of your single stranded oligonucleotides to our gold nanoparticles and quantum dots. Our services include: 1. Synthesis of your single-stranded oligonucleotides for conjugation. Supply us with your sequence, and which terminal (5' or 3') to be attached to the nanoparticle surface and we do the rest. 2. Conjugation of your oligonucleotide to a nanoparticle of your choice. 3. Purification of conjugate. Conjugation to small molecules/ dyes Apollo Biolife provides custom conjugation of your protein or antibody to small molecule or a fluorophore/ chromophore. Our services include: 1. Conjugation of 1-5mg of protein. 2. Purification of your protein conjugate using size exclusion chromatography. 3. Characterization of your labeled protein. Order Information Custom Protein-Nanoparticle Conjugation Custom Oligonucleotide-Nanoparticle Conjugation Custom Protein- Organic molecule Conjugation 11 P a g e

13 Custom Cloning Service Cloning and sequencing of PCR products are of major importance in Gene Cloning Experiments. It can, however, sometimes be difficult and time-consuming. Our Custom Services team can quickly resolve these difficulties for you, so you can skip the technical hurdles, focus on the biology, and expedite end-results. Features: Sequencing of amplified product using amplification primers does not read the terminal bases on both ends. We provide complete bi-directional sequence data for the amplified product. Amplified products that are otherwise difficult to clone, are cloned and sequenced. Sequence data analysed, aligned and complete data provided. Deliverables Cloned DNA (up to 1-2 µg) Sequence data Project Report Delivery time 4-6 weeks Order Information Custom Cloning and sequencing 12 P a g e

14 Microbial Identification Service Microbial identification based on the gene sequencing has become the important tool for microbiologists. Apollo Biolife s microbial identification service provides high quality, confidential service microbial identification of bacteria, fungi and yeasts for our clients. We serve clients from a range of industries including the pharmaceutical, food and manufacturing sectors, as well as research organisations working in agriculture, horticulture, quarantine, biodiversity assessment and biological control. We offer following microbial identification services based on DNA sequencing. Identification up to species level and sometimes strain level is possible by this method. 13 P a g e Bacterial Identification Service It is based on 1500 bp 16S rrna gene sequence. It involves amplification of 16S rrna gene and sequencing of the PCR product Actinomycetes Identification Service It is based on Actinomycetes species specific 750 bp 16S rrna gene sequence. Fungal Identification Service It is based on 650 bp ITS region of the rrna gene sequence. Yeast Identification Service It is based on 500 bp ITS region of the rrna gene sequence Features: Fast turnaround time. Delivery time-3 to 4 weeks Sample Requirements Pure microbial culture Deliverables: Final report containing microbial identity information. Nucleotide sequence alignment within 10 most similar neighbours. Phylogenetic tree displaying evolutionary relationship of 10 nearest neighbours. Order Information Bacterial and Actinomycetes Identification Service Fungal and Yeast Identification Service

15 Protein Purification Service Years of solid experience and unique knowledge in protein expression and purification services of our team allows Apollo Biolife to be your reliable source for functional proteins, to help you break the bottlenecks in protein expression and purification. We routinely work with antibodies, antigens, enzymes, DNA binding proteins, membrane proteins, blood proteins and many more. Your Protein Purification Services start with the analysis of physico-chemical and biological properties of a target protein resulting in the development of tailored procedures for its extraction, purification and characterization. Our purification strategy aims to achieve a homogeneous active protein preparation in two to three purification steps. This goal is reached by a thorough selection and optimization of the capture step, incorporation of a gel filtration step to remove aggregates, degradation products and other contaminants, selection of buffer conditions that stabilize biological activity and prevent product degradation. Features purification of fusion and tagged proteins from recombinant sources native proteins from natural sources antibodies of different isotypes including IgMs membrane proteins any protein purification mode can be used ion-exchange gel filtration affinity (broad-spectrum) hydrophobic interaction Efficiency is provided by the automation and precision of AKTA systems from Amersham Biosciences (currently GE) Deliverables 1. SDS-PAGE and/or dot/western blotting gel picture. 2. Final products are supplied with a certificate of analysis, purification report and, if applicable, a batch record. Order Information Custom Protein Purification Service 14 P a g e

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