SOX2 antibody - Embryonic Stem Cell Marker (ab15830) datasheet

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1 -Embryonic Stem Cell Marker (ab15830) 1/8 ページ d Products: Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells SOX2 antibody - Embryonic Stem Cell Marker (ab15830) datasheet Product Name Product type Description Immunogen Reacts with Predicted to react (species key) Tested applications (see key) SOX2 antibody - Embryonic Stem Cell Marker Primary antibodies Rabbit polyclonal to SOX2 - Embryonic Stem Cell Marker Synthetic peptide conjugated to KLH derived from within residues of Human SOX2. (Peptide available as ab15831.) Hu, Ms, Rat, Chk, Ql, Zfsh, Dfsh Pfsh, Shp, Xl (Due to sequence homology) ChIP, ICC/IF, IHC-Fr, IHC-P, WB Abreviews Reviews by Abcam customers (see key) Read about Abreviews (Read all Abreviews) Submit an Abreview and earn up to 220 Abpoints by reviewing this product ChIP 1 Abreview - average rating: ICC/IF 2 Abreviews - average rating: IHC-P 6 Abreviews - average rating: IHC-Fr 1 Abreview - average rating: WB 2 Abreviews - average rating: IHC-P (read Abreview) IHC-P (read Abreview) IHC-P (read Abreview) IHC-P (read Abreview) Application notes (see key) Recommended dilutions ChIP: Use at a concentration of 4 袖 g/ml. ICC/IF: Use at a concentration of 3 袖 g/ml. IHC-P: 1/100-1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IHC-Fr: Use at an assay dependent dilution. WB: Use at a concentration of 0.3 袖 g/ml. Detects a band of approximately 37 kda (predicted molecular weight: 34 kda). Positive control (see definition) Cellular localization Research areas Relevance Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. ICC: human ES cells (H9 line) WB: mouse or human embryonic stem cell lysate, Mouse Embryonic Germ Ce lysate Nuclear Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells Stem Cells >> Embryonic Stem Cells >> Intracellular Neuroscience >> Growth & Development >> Neurogenesis Nuclear Signaling >> Transcription >> Domain Families >> HMG Box Neuroscience >> Growth & Development >> Signal Transduction Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zippe >> Leucine Zipper Neuroscience >> Neuronal Markers >> Neural Stem Cells SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA

2 -Embryonic Stem Cell Marker (ab15830) 2/8 ページ d helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution. Database links Entrez Gene (Chicken) 6657 (Human) (Mouse) (Zebrafish) SwissProt P48430 (Chicken) P48431 (Human) P48432 (Mouse) P54231 (Sheep) GeneCard Omim GC03P (Human) (Human) Unigene 601 (Chicken) 816 (Human) 4541 (Mouse) Raised in Clonality Isotype Purity Storage buffer Form Concentration Storage instructions Rabbit Polyclonal IgG Immunogen affinity purified Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, ph 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS Liquid Batch dependent within range: mg/ml Aliquot and store at -20 属 C or -80 属 C. Avoid repeated freeze / thaw cycles. At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SOX2 antibody - Embryonic Stem Cell Marker is on this datasheet. But please do contact us if you would like any reassurance! See below for SOX2 antibody - Embryonic Stem Cell Marker images, references, products related to ab15830 and other tools. SOX2 antibody - Embryonic Stem Cell Marker images: Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

3 -Embryonic Stem Cell Marker (ab15830) 3/8 ページ d Sox2-specific antibody, ab15830, stained the nucleus of human ES cells (H9 line). Upon differentiation into neurones ab15830, but not Oct4-specific antibody, stained the nuclei (data not shown). Mouse embryonic fibroblasts and ES cells differentiated into early mesoderm were not stained by ab Dilution used: 5µg/ml. Ludovic Vallier, University of Cambridge - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

4 -Embryonic Stem Cell Marker (ab15830) 4/8 ページ d Lanes 1-2 : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 µg/ml Lane 1: 20 µg Mouse embryonic stem cell lysate Lane 1: 20 µg Human embryonic stem cell lysate ab15830 recognised a band of the expected size (34 kda) in human and mouse embryonic stem cell lysates. Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab15830) All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 0.3 袖 g/ml Lane 1 : Mouse Embryonic Stem Cell lysate Lane 2 : Mouse Embryonic Germ Cell lysate Lane 3 : Mouse Embryonic Fibroblast Lysate Predicted band size : 34kDa Observed band size : 37 kda (why is the actual band size different from the predicted?)

5 -Embryonic Stem Cell Marker (ab15830) 5/8 ページ d Petra Hajkova, University of Cambridge Western blot All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 袖 g/ml Lane 1 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202) Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with SOX2 peptide (ab15831) at 1 袖 g/ml Lane 5 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202) with SOX2 peptide (ab15831) at 1 袖 g/ml Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) with SOX2 peptide (ab15831) at 1 袖 g/ml Lysates/proteins at 10 袖 l per lane. Observed band size : 42 kda (why is the actual band size different from the predicted?) A band corresponding to Sox2 was detectable in 46C Sox1-expressing Mouse Neural Progenitor Cell Lysate (ab27205), IOUD2 Oct4-expressing Mouse Embryonic Stem Cell Lysate (ab272020) and F9 Mouse Embryonic Carcinoma Cell Lysate (ab27193). This band was detected in the lysate by Western Blotting using anti-sox2 antibody ab The Sox2 bands were specifically blocked using the immunising peptide of ab An Oct4 band was also detectable in the 47C lysate by Western Blotting (data not shown). It has not been possible to find a suitable Sox1-specific antibody able to detect Sox1 in 46C by Western Blotting. However, we are confident that 100% of the cells (excluding feeders) that contributed to the lysate were actively expressing Sox1. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

6 -Embryonic Stem Cell Marker (ab15830) 6/8 ページ d SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Formalin/PFA-fixed paraffin-embedded adult zebra fish spinal cord sections. Antigen retrieval step: Heat mediated. 1%BSA as blocking agent for 10 mins at RT 属 C. ab15830 used at 1/600 for 2 hours.secondary Antibody: Biotin conjugated goat anti rabbir IgG used at 1/600. Carl Hobbs, KCL, UK Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830) Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Dogfish/Catshark Tissue sections. Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/600 incubated for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti IgG. Carl Hobbs, CARD, KCL, London, UK

7 -Embryonic Stem Cell Marker (ab15830) 7/8 ページ d Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830) Immunohistochemistical detection (on formaldehyde/pfa-fixed paraffin-embedded sections) of SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Quail Tissue sections (brain stem). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab15830 incubated at 1/600 for 2 hours. Secondary Antibody: Biotin labelled goat anti rabbit IgG. This image shows nuclear expression in the ventricular region of the embryonic quail brain stem. Carl Hobbs, CARD, KCL, London, UK Customer reviews (feedback) regarding SOX2 antibody - Embryonic Stem Cell Marker Customer enquiries (FAQ's) regarding SOX2 antibody - Embryonic Stem Cell Marker Protocols for SOX2 antibody - Embryonic Stem Cell Marker Price and availability of products related to SOX2 antibody - Embryonic Stem Cell Marker SOX2 antibody - Embryonic Stem Cell Marker - related products: Products for ChIP ChIP Related Products Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) Slides Products for Western blot Loading controls Lysates Immunizing Peptide for SOX2 antibody - Embryonic Stem Cell Marker ab15831 SOX2 peptide Compatible Secondaries for SOX2 antibody - Embryonic Stem Cell Marker ab6564 Cy5 速 antibody ab6717 FITC antibody ab6720 Biotin antibody ab6721 HRP antibody ab6722 Alkaline Phosphatase antibody

8 -Embryonic Stem Cell Marker (ab15830) 8/8 ページ d ab6793 ab7007 ab7019 ab27234 ab27235 ab27236 ab27237 ab27238 ab27239 ab27240 ab31193 ab60314 ab60315 ab60317 ab60319 Texas Red 速 antibody Phycoerythrin antibody Agarose antibody 10nm Gold antibody 5nm Gold antibody 15nm Gold antibody 20nm Gold antibody 30nm Gold antibody 10nm Gold antibody 5nm Gold antibody Duck polyclonal to Rabbit IgG Chromeo 痰 488 antibody Chromeo 痰 494 antibody Chromeo 痰 546 antibody Chromeo 痰 642 antibody Other SOX2 Antibodies See all SOX2 antibodies (2)... All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" contact us with your comments Abcam plc. All rights reserved. Terms and Conditions Legal Privacy Policy Investor Relations Contact Us

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