IN VITRO THIOCARBAMATE RESISTANCE OF TRICHOPHYTON MENTAGROPHYTES
|
|
- Julian Dickerson
- 6 years ago
- Views:
Transcription
1 IN VITRO THIOCARBAMATE RESISTANCE OF TRICHOPHYTON MENTAGROPHYTES Hongo, Bunkyo-ku, Tokyo 113, Japan (Received November 17, 1989' Accepted February 1, 1990) vitro Trichophyn mentagrophytes piritetrate (M-732), a new thiocarbamate antidermaphytic compound, studied comparison with a reference thiocarbamate, lnaftate. It found that this fungus not readily able develop eir se compounds. After a long period serial subculturg six stras Sabouraud dextrose broths on Sabouraud dextrose agar slants contag creasg concentrations compound, only one stra each eventually became highly resistant lnaftate eir media. None stras acquired piritetrate. two lnaftate-resistant stras thus obtaed manifested mphological, biochemical pathological changes showed partial cross- piritetrate. Key wds: Piritetrate (M-732), Tolnaftate, Thiocarbamate, Trichophyn mentagrophytes INTRODUCTION Piritetrate (M-732) is a new thiocarbamate compound [methyl (6-methoxy-2-pyridyl) carbamothioic acid 0-5, 6, 7, 8-tetrahydro-2- naphthalenyl ester; C18 H20 N2O2 S] which had been synsized by Chemical Research Labary Tosoh Cpation (fmerly, Toyo Soda Manufacturg Co., Ltd.), SHINNANYO- SHI, YAMAGUCHI-KEN, J APAN. structure piritetrate is shown Fig. 1, ger with that a reference thiocarbamate, lnaftate [methyl (3-methylphenyl) carbamothioic acid O-2 -naphthalenyl ester; C19H17N0S]1). Piritetrate is a white crystalle powder with a meltg pot t. It is soluble water, slightly soluble n -hexane, methanol ethanol, readily soluble benzene, er, acene, chlm, dimethylsulfoxide N, N-dimethyl- Piritetrate Tolnaftate (M-732) Fig. 1. Structures piritetrate (M-732) lnaftate. fmamide. potent vitro vivo antidermaphytic activity piritetrate had been itially
2 CHEMOTHERAPY MAY 1990 identified our screeng program on a number members thiocarbamate series which had been synsized as agricultural herbicides by Chemical Research Labary. Later2), we described detail results comparison vitro antifungal activity piritetrate, lnaftate, lciclate (a recently developed thiocarbamate compound; [O-(1,2,3,4-tetrahydro-1,4- methanonaphthalen-6- yl m-n- dimethylthiocarbanilatei3) clotrimazole (a classic azole compound; [1-{(2- chlophenyl) diphenylmethyl) imidazolej4'5), found that piritetrate possessed strongest antidermaphytic activity among se antifungals. In same paper, we also demonstrated me potent vivo activity piritetrate than lnaftate dermal model fections guea pigs with T. mentagrophytes. Me recently6), we repted action mechanism piritetrate compared with that lnaftate fmer a much me potent hibir fungal squalene epoxidation. This paper deals with results comparative vitro studies conducted on piritetrate lnaftate agast Trichophyn mentagrophytes. (A prelimary rept this wk presented at 24 th Interscience Conference on Antimicrobial Agents Chemapy, Ocber 8 `10, 1984, WASHINGTON, D. C.) MATERIALS AND METHODS Anhfungals. Piritetrate lnaftate supplied by Tosoh Cpation. se compounds dissolved dimethyl sulfoxide (DMS0), sck solutions 20 mg/ml kept at 4 Ž dark. Twold dilutions test media prepared from se sck }olutions. Trichophyn mentagrophytes stras. Six stras, namely, MTU numbers 19021, 19023, 19024, 19025, VUT 74023, used. se had been mataed our labaries by subculturg on Sabouraud dextrose agar (SDA; 2% dextrose, ph 6.5 }) slants. None m known have been exposed drugs, cludg piritetrate lnaftate, vitro vivo. Stra VUT had been isolated from a naturally-fected guea pig identified as Arthroderma vanbreuseghemii by Dr. H ASEGAWA A, Animal House, Faculty Agriculture, University Tokyo. Pri present study, we reconfirmed that this stra highly virulent f guea pigs upon dermal fection produced ascomyceus fm. Later, it proved be only stra which developed lnaftate. Stra MTU 19025, which had been isolated by us from a patient with tea pedis, representatively selected f a comparative study primary with stra VUT Stras MTU VUT highly susceptible both piritetrate lnaftate, with each showg MICs 0.01 gg/m ug/ml Sabouraud dextrose broth (SDB; 2% dextrose, ph 6.5 }) 0.02ƒÊg/ml 0.1ƒÊg/ml on SDA, respec. tively. Media. SDA SDB used f various tests. se will hereafter be simply called solid medium liquid medium, respectively, unless wise specified. 'Ordary Sabouraud dextrose agar' (hereafter referred as dary SDA; 4% dextrose, ph 6.5 }) employed obta a sufficient amount conidia f oculation tests on mphological biochemical properties as well as on virulence resistant. F demonstration perfect stage stra VUT its subcultures, Takashio's medium') used its gredients are neopepne (Difco) 0.1 g, dextrose 0.2 g, KI-121)04 0.1g, MgSO4 E7 H g, Bacagar 2.0g, made up 1,000 ml with distilled water. Animals. Male guea pigs, weighg ca. 300g, purchased from Shizuoka Labary Animals Cpation, Ltd., S HIZUOKA, JApAN used f determation virulence subcultures comparison with ir sensitive stras. Preparation oculum. An appropriate volume sale contag 0.1% Tween 80 poured on culture T. mentagrophytes grown on dary SDA slants at 27t f 4 weeks, surface culture scratched with a platum loop free conidia. conidium suspension mixed with a small amount hphae transferred a flask allowed
3 VOL. 38 NO.5 Thiocarbamate st f an appropriate period until small pieces precipitated. After filterg suspension, excludg precipitates through sterile folded gauze, supernatant, contag almost pure conidia, suspended same sale adjusted ca. 10 CFU/ml conidia on basis an optical density 0.35 at 540 mp. Immediately pri oculation, conidium suspension shaken gently ensure a homogeneous state. Determation MICs. MICs piritetrate lnaftate determed by broth agar dilution methods. Conidia oculated 5 ml broth L- fm tubes on 20 ml agar plates contag twold dilutions antifungal. oculum sizes ca. 103 CFU/ml ca. 103 CFU, respectively. After cubation at 27 Ž f one week liquid medium with shakg, f 3 weeks on solid medium, MICs read. Testg f development. F development liquid medium, th culture, which had grown fairly well at a subhibiry concentration closest MIC, gently crushed a mtar obta a homogeneous oculum suspension adjusted ca. 107 CFU/ml. conidium suspension n oculated a series same a little higher twold dilutions antifungal. Subculturg at 27t with shakg perfmed every week f 27 weeks. At time transfer solid medium cultures, conidia harvested from colonies grown on plates contag antifungal at a subhibiry concentration closest MIC. Subculturg carried out every 3 weeks, 27 times tal. oculum sizes liquid medium on solid medium same as those dicated determation MICs. Testg f demonstration primary. existence number cells resistant 1ƒÊg/ml compounds. number visible colonies fmed after cubation f one, week at same temperature counted. Demonstration back mutation. two lnaftate-resistant which eventually obtaed passed 10 times liquid medium absence antifungal. level checked after 5 10 passes determe if back mutation occurred. Demonstration cross-. Two resistant ir stra (VUT 74023) examed f cross- piritetrate lnaftate terms MIC on plates contag appropriate concentrations antifungal. Testg f cultural mphological changes resistant. To exame f possible cultural mphological changes, cubated at 27t liquid medium with shakg on dary SDA plates, ir growth rates colony fms observed over a period 4 6 weeks, respectively, comparison with ir stra. Cells grown dary slide culture usg SDA at same temperature also compared with those ir stra by means light microscopy. Possible loss ascomycemaus fm examed cultures resistant grown on Takashio's medium at 27t f 2 months. Testg f changes virulence resistant. resistant compared with ir stra f ir virulence each ten guea pigs. An area (ca. 8 by 10 cm) on dsal trunk each animal shaved, two circular areas 3 cm diameter separated by an appropriate distance gently abraded with spaper. About 106 CFU conidia se stras grown on dary SDA at 27 Ž f 5 weeks n oculated on se treated sites. both compounds population stras VUT MTU determed. About 109 CFU conidia harvested from each stra after growth on dary SDA at 27t f 4 weeks plated on plates contag 0, 0.01, 0.1 RESULTS Development. Five T. mentagrophytes stras, i.e., 19021, 19023, 19024, , did not develop- eir compound
4 CHEMOTHERAPY MAY 1990 Fig. 2. Development stras Trichophyn mentagrophytes piritetrate lnaftate liquid medium. Initial susceptibility stra VUT piritetrate lnaftate geml, while fal susceptibility 0.04 >80ƒÊg/ml. respective figures f stra MTU ƒÊg/ml ƒÊg/ml. Fig. 4. Comparison distribution piritetrate- lnaftate-resistant mutant cells existg sensitive stras Trichophyn mentagrophytes VUT MTU Ca. 109 CFU conidia stras grown on SDA slants by cubatg at 27 Ž f 4 weeks plated on SDA plates contag drug at gem' visible colonies fmed after cubatg f one week at same temperature counted. Symbols: A, piritetrate; B, lnaftate; VUT stra,;------, MTU stra. drug late stage serial subculturg on solid medium. se resistant progenies designated L-1 mutant mutant, respectively. We consider that both progenies reached highest-level, over an 800-fold crease, because y grew at 80mg/ml, highest tested concentration lnaftate its maximum solubility Fig. 3. Development stras Trichophyn mentagrophytes piritetrate lnaftate solid medium. Initial susceptibility stra VUT piritetrate lnaftate jig/ml, while fal susceptibility 0.08 >80 jig/ml. respective figures f stra MTU ƒÊg/ml ƒÊg/ml. eir medium. ir MIC values creased only 2-4-fold. Stras showed a transient 8-fold crease,piritetrate lnaftate, respectively, liquid medium. In contrast, only stra MT developed high-level olnaftate both media. This stra developed gradual lnaftate midway through series subculturg liquid medium progressive media used (piritetrate also). F simplicity, Figs. 2 3 show results f stra MTU 19025, selected as representative five stras which did not develop, stra VUT Distribution resistant mutant cells existg sensitive stras. Usg about 109 CFU conidium stras VUT MTU 19025, all cells stra MTU sensitive 0.1ƒÊg/ml less both compounds, whereas relatively many cells stra VUT a little less sensitive lnaftate, with MICs between 0.1 1ƒÊg/ml (Fig. 4). This difference susceptibility piritetrate lnaftate, although not very conspicuous, may evidence fact that stra VUT eventually developed a high level lnaftate durg repeated subculturg
5 VOL. 38 NO.5 Thiocarbamate Table 1. Degree back mutation 439 L1 S 1 lnaftate-resistant Trichophyn rnentagrophytes VUT Resistant obtaed by serial transfers stra sensitive piritetrate lnaftate liquid on solid medium contag creasg concentrations L-I: Tolnaftate-resistant drug (see Fig. 1.). mutant obtaed a SDB; tested f back mutation by serial transfers drug-free SDB. : Tolnaftate-resistant mutant b obtaed on SDA; tested f back mutation by 10 serial transfers Table 2. Piritetrate See liquid medium five failed mutant its Cross 2. re between grow at a even after 10 mutant, its itial on solid piritetrate slight results medium but are defite lnaftate L-1 80ƒÊg/ml whereas after did stra susceptibility,. 1, not susceptibility not MIC after changed 0.08 test 5 revert even had which however, igal ir transfers, its contrast Table concentration VUT compound. level lnaftate-resistant , eir shown highest much transfers. from completely As MTU SDA. mentagrophytes Table medium, lost transfers. 10 lnaftate mutant solid sensitivity Trichophyn footnote cludg. retaed able on stras, develop Stability on drug-free ttg/ml. f presented cross Table cross- both L-1 Fig. 5. Colonies mutant (resistant lnaftat; left) its stra (VUT 74023; right). Note pigment fmation by mutant.
6 CHEMOTHERAPY 440 Fig. 6. Light stra. cell with medium f different, brown did As seen streakg a 2 pm shown conidia whereas 6 globose. that ospes spiral with fmed stra. reverse deeper hyphae 0.3 size stra. resistant also 0.7 shape. pm Few no aleuri- its shape. than macroconidia by on antifungal agents duction resistant is much me difficult than with bacteria. has thus received little attention, contrast critical imptance bacterial. Dermaphytes versus antifungals are no exception this generalization; re have been only a few repts on ir, ambrutic fmation conspictus 1 segmen- variants Fungal number Meover, ovoid, hyphae me as stra conspicuous a 1.5 produced shape globose caused hyphae by size human pathogenic fungi dicate that naturally -occurrg is very rare, with notable exception flucyset1. rangg stra, Most repts with width i.e., compared various characterized conidia, mutant. easily (/400) stras positive tissue mycological response at fected foci under same test conditions. oculated much disrted tation f f stra. ten became diameter, conidia entirely 2 pm longer left) two DISCUSSION diameter Fig. 5, Fig. size 5 pm reddish- colonies pigs given a dermal oculation ca. 106CFU conidia two at shaved sites on back showed no recognizable lesions at any 20 fected foci each tal both groups, while group stra gave clearly colonies a plate greater 2.5 (s); solid lnaftate; obverse. Microscopically, from ir deeper liquid on between Genetic property resistant. Both resistant cubated on Takashio's medium found have completely lost ability produce a perfect stage durg 3month observation period. Virulence resistant. Ten guea significantly from seen suspension on than not fmation conidial fms 27 Ž week differ pigment ne. where one change period, col by (resistant differences resistant colony not soluble col mutant at f prcipal cubation weeks, mutant rates shakg by Note cubated six stra. area mphologies when medium cells right)( ~400). growth, 74023;. Cultural microscopy (VUT MAY 1990 such as griseulv9-16) (cyclopropylpyran acid) 17.18) However, view widespread frequent use antifungal agents recent years, studies have become newly exploited imptant, agents. particularly with
7 VOL. 38 NO.5 Thiocarbamate In this study we found that only one six test stras T. mentagrophytes became resistant lnaftate after relatively many transfers, while none acquired piritetrate. se results suggest that this fungal species, probably dermaphytes as well, do not readily develop se thiocarbamate compounds. However, stra VUT might not necessarily be such an exception its acquisition lnaftate view repts on primary / secondary antifungals such as azoles pathogenic fungi, cludg dermaphytes ). possible existence a few stras which are able acquire such thiocarbamates durg longterm rapy might be responsible f occasional cases unsuccessful treatment dermaphyses. It is notewthy that acquired lnaftate-resistant three ders magnitude greater than stra. test stras, cludg stras MTU VUT 74023, transiently developed 8-fold less liquid medium on solid medium. However, such a transient rise MIC values cannot be regarded as acquisition true sense, sce evaluated exceed upper limit MIC range. MIC values do not It seems likely that se multi-step obtaed by means serial subculturg presence creasg concentrations lnaftate obviously differ from one-step resistant flucyse ir mode acquirg 8,22). That acquisition me progressive on solid than liquid medium might be due various facrs, f stance, physiological conditions both media f growth, preparation cell suspensions f oculation, etc. Overall, VUT MTU showed similar cell population structural patterns terms susceptibility piritetrate lnaftate befe transfer. However, a culture VUT cluded me cells less sensitive lnaftate than piritetrate. This difference considered small but not negligible sce VUT eventually became resistant lnaftate but not piritetrate by repeated subculturg eir medium. reasons why mutant became resistant earlier than L-1 mutant, yet L-1 mutant me stable retention than S- 1 mutant, are unclear. Possible facrs are slight differences techniques f subculturg f development f back mutation, stra variation. fdg that cross- lnaftate-resistant piritetrate partial may derive from differences chemical structures se thiocarbamates hence ir vitro vivo antidermaphytic activity : piritetrate has a pyride nucleus with a methoxy group tetrahydronaphthalene moiety, while lnaftate has a benzene nucleus with a methyl group naphthalene moiety. It is particular terest that acquisition stra VUT lnaftate accompanied by a characteristic pigment production matured colonies its mutant. pigment might be a carotenoid (s) consideration its col solubility solid medium. Changes cellular mphology two resistant also characteristic, f stance, enlargement conidia, strikg segmentation hyphae that produced a great many aleuriospes, accelerated fmation spiral hyphae, etc. se features might be due qualitative quantitative alterations imptant cellular constituents such as nucleic acids prote, as repted f fungal cells treated with flucyses). loss perfect stage resistant ir concomitant loss reduction virulence f guea pigs upon dermal fection with conidia might be due a lowerg ir gemral metabolic activity. This 'loss mutation' found be irreversible both. Thus it ic clear that se changes mphological, r hysioi ogic a 1 pathological properties lnaftate-resistant closely related each. In summary, a new thiocarbamate compound,
8 CHEMOTHERAPY MAY 1990 piritetrate, a reference thiocarbamate, lnaftate, did not readily duce T. mentagrophytes stras vitro by serial transfer both liquid on solid medium contag creasg drug concentrations. However, one six test stras became resistant lnaftate after many transfers. se results suggest possible failure long-term rapy with such thiocarbamates if resistant cells develop. characteristic mphological changes, concomitant loss perfect stage virulence, cross stability acquired two resultant lnaftate-resistant are described. ACKNOWLEDGMENTS We thank Dr. HASEGAWA A, Animal House, Faculty Agriculture, University Tokyo, TOKYO Tosoh Cpation f providg stra VUT T. mentagrophytes two thiocarbamate compounds, respectively. We also thank Tomoko Kamoshida f her technical assistance. REFERENCES 1) NOGUCHI T, Krul A, IGARASHI Y, SHIGEMATSU A, TANIGUCHI K. Antitrichophyn activity naphthiomates. Antimicrob Agents Chem 1962: , ) IWATA K, YAMASHITA T, UEHARA H: In vitro vivo activity piritetrate (M-732), a new antidermaphytic thiocarbamate. Antimicrob Agents Chem 33: , ) de CARNERI I, Mown G, B IANCHI S, CASTELLINO S, MEINARDI G, MANDELLI V: Tolciclate agast dermaphytes. Arzneim-Fsch (Drug Res) 26: 769 `772, ) PLEMPEL M, BARTMANN K: Experimental studies on antimycotic action clotrimazole (Canesten) vitro after local application vivo. Drugs Germ 15 : , ) SMITH K J, WARNOCK D W, K ENNEDY C T, J OHNSON E M, H OPWOOD V, VAN C UTSEM J, VANDENBOSSCHE H: Azole Cida albicans. J Med Vet Mycol 24: , ) M ORITA T, IWATA K, NOZAWA Y: Inhibiry effect a new mycotic agent, piritetrate on ergosterol biosynsis pathogenic fungi. J 7) TAKASHIO M: Sexual reproduction some Arthroderma Nannizzia on diluted Sabouraud agar with without salts, Mykosen 15: 11 17, ) S CHOLER H J: Flucyse, pp Antifungal Chem., Speller D C E, ) ARTIS W M, BONNIE M, Jos H E Griseulvresistant dermaphysis crelates with vitro. Arch Dermal 117: 16 19, ) A YTOUN R S C, CAMPBELL A H, NAPIER E J, SEILER D A L: Mycological aspects action griseulv agast dermaphytes. Arch Dermal 81: , ) DAVIES R R, EVERALL J D, HAMILTON E: Mycological clical evaluation griseulv f chronic onychomycosis. Br med J iii: , 1967 ` 12) GREENBERG J H: Griseulv. Int J Dermal 18: 249 `260, ) GRIN E I, NADAZDIN M, OzwGOVIC L: Investigations dermaphyte sensitivity griseulv. Acta Derm Venerol 45: 283 `287, ) H ANTSCHK D, GoETZ H: Griseulv Resistanz. Z Hautkr 56 : 1326 `1333, ) LENHART K: Griseulv-resistant dermaphytes: II Physiological genetic studies. Mykosen 13: 139 `144, ) YOUNG C N: Sensitivity patterns griseulv Trichophyn rubrum rgwm fungi. Trans Rep St John's Hosp Derm Soc 58: 226 `234, ) RINGEL S M, GREENOUGH R C, RoaMEx S, Coo D, Gurr A L, B LAIR B, KANTER G, STRANDTMANN M: Ambrutic (W7783), a new antifungal antibiotic. J Antibiol 30: , ) S HADOMY S, DIXON D M, ESPINEL-INGRcFF A, WAGNER G E, Yu H P, SHADOMY H J: In vitt v studies with ambrutic, a new antifungal antibiotic. Antimicrob Agents Chem 14: , )HOLT R J, Amu A: Miconazole-resistant cida. Lancet i:50, ) JOHNSON E M, RICHARDSON M D, WARNOCK D W: In-vitro imidazole antifungals Cida albicans. J Antimicrob Chem 13: 31 43, ) RYLEY J F, WILSON R G, BARRETT-BEE K J: Azole Cida albicans. Sabouraudia 22:53 63, ) HAMILTON -MILLER J M T: Physiological properties mutagen-duced variants Cida albicans resistant polyene antibiotics. J Med Microbiol 5: , 1972 Med Vet Mycol 27: 17 25, 1989
9 VOL. 38 NO.5 Thiocarbamate
LESSON ASSIGNMENT. After completing this lesson, you should be able to: Identify principles for maintaining a "working" stock culture.
LESSON ASSIGNMENT LESSON 10 Maintaining Stock Cultures. TEXT ASSIGNMENT Paragraphs 10-1 through 10-6. TASK OBJECTIVES After completing this lesson, you should be able to: 10-1. Identify principles for
More information21.4 Recombinant DNA technology Calculation worksheet. AQA Biology. Calculating the efficiency of DNA transfer during genetic engineering
Calculating the efficiency of DNA transfer during genetic engineering Specification references 3.8.4.1 MS 0.1, MS 0.3 Learning outcomes After completing this worksheet you should be able to: manipulate
More informationExercise 19. Fungi: Molds and Yeasts F10 Or The Rotten World Around Us
Exercise 19 119 Fungi: Molds and Yeasts F10 Or The Rotten World Around Us INTRODUCTION: Student Learning Objectives: After completing this exercise students will: a. Define the terms Saprophyte, Mycosis,
More informationAntifungal Susceptibility testing: New trends. Abstract: Amina Mostafa Abdel Aal, Mohamed M. Taha*, Noha El-Mashad and Walaa El-Shabrawy
Antifungal Susceptibility testing: New trends Amina Mostafa Abdel Aal, Mohamed M. Taha*, ha El-Mashad and Walaa El-Shabrawy Egyptian Dermatology Online Journal 3 (1): 1, June, 2007 Departments of: Clinical
More informationDetermination of Pseudomonas aeruginosa by Biochemical Test Methods Test, a Modified Biochemical Test for
Japan. J. Microbiol. Vol. 14 (4), 279-284, 1970 Determination of Pseudomonas aeruginosa II. Acylamidase by Biochemical Test Methods the Identification Test, a Modified Biochemical Test for of Pseudomonas
More informationobtained from the infected and treated tissues, Fleming's2 technic of hemolytic streptococcus B. Immediately following the infection, 1.0 ml.
THE SENSITIVITY OF STREPTOCOCCI TO PENICILLIN G AFTER EXPOSURE TO THE ANTIBIOTIC IN VIVO* E. GRUNBERG, C. UNGER, AND D. ELDRIDGE Previous investigations by Grunberg, Schnitzer, and Unger3 on the topical
More informationPRESERVATIVE EFFICACY TEST FOR COSMETIC PRODUCT
1 SCOPE AND FIELD OF APPLICATION To determine the efficacy of the antimicrobial activity of preservatives used in cosmetic products. The method covers the determination of the suitability of preservation
More informationAnticandidal Activities of Terconazole,
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, June 1986, p. 986-991 0066-4804/86/060986-06$02.00/0 Copyright 1986, American Society for Microbiology Vol. 29, No. 6 Anticandidal Activities of Terconazole, a Broad-Spectrum
More informationAntifungal drug response in an in vitro model of dermatophyte nail infection
Medical Mycology April 2004, 42, 159 /163 Antifungal drug response in an in vitro model of dermatophyte nail infection C. S. OSBORNE*$, I. LEITNER*, B. FAVRE% & N. S. RYDER*$ *Infectious Diseases Department,
More informationfatigue and tensile tests were cut parallel to the rolling direction into the forms indicated in Fig. 2.
Stress Cold-Rolled Fatigue Carbon By Masahiko Strength Steel Ogirima* To obta effect cold rollg on fatigue strength, commercial 0.8% carbon eutectoid steel coldrolled to various degrees n fatigued bendg
More informationStandardization of Antifungal Susceptibility Variables for a Semiautomated Methodology
JOURNAL OF CLINICAL MICROBIOLOGY, July 2001, p. 2513 2517 Vol. 39, No. 7 0095-1137/01/$04.00 0 DOI: 10.1128/JCM.39.7.2513 2517.2001 Copyright 2001, American Society for Microbiology. All Rights Reserved.
More informationTransformation: Theory. Day 2: Transformation Relevant Book Sections
Day 2: Transformation Relevant Book Sections We will follow the protocols provided in various industry-standard kits, instead of the protocols described in these chapters, but the chapters provide good
More informationIn vitro susceptibility testing of amorolfine in pathogenic fungi isolated from dermatomycosis patients in China
Original article In vitro susceptibility testing of amorolfine in pathogenic fungi isolated from dermatomycosis patients in China In vitro Empfindlichkeitsprüfung mit Amorolfin an pathogenen Pilzen von
More informationON THE DEVELOPMENT OF STRAINS OF BACTERIA RESIS-
214 ON THE DEVELOPMENT OF STRAINS OF BACTERIA RESIS- TANT TO LYSOZYME ACTION AND THE RELATION OF LYSOZYME ACTION TO INTRACELLULAR DIGESTION. ALEXANDER FLEMING, F.R.C.S., AND V. D. ALLISON, M.D. From the
More informationIsolation of Lac+ Mutants from a Lac- Strain of Escherichia coli, by the Replica Plating Technique
586 BANI^, S. (1958). J. gen. Microbiol. 18, 586-590 Isolation of Lac+ Mutants from a Lac- Strain of Escherichia coli, by the Replica Plating Technique BY S. BANIC Institute of Microbiology, Medical Faculty,
More informationMethod for the Detection of Xanthomonas spp. on Pepper Seed
Method for the Detection of Xanthomonas spp. on Pepper Seed Crop: Pathogen: Pepper (Capsicum annuum) Xanthomonas euvesicatoria, Xanthomonas vesicatoria and Xanthomonas gardneri See (1) and (2) for an account
More informationINVITRO COMPATIBILITY EVALUATION FOR THE BIOCONVERSION OF DOMESTIC SOLID WASTES BY MIXED CULTURES OF MICRO-ORGANISMS
INVITRO COMPATIBILITY EVALUATION FOR THE BIOCONVERSION OF DOMESTIC SOLID WASTES BY MIXED CULTURES OF MICRO-ORGANISMS Ashwitha Kodaparthi 1, Pavan Kumar Pindi 2, A. Swaroopa Rani 3 1 Department of Microbiology,
More informationEffects of Copper and Phosphorus on Temper Embrittlement of Mn-Mo-Ni Low Alloy Steel (ASTM A533-B)* NobuharuKusunoki***and KazuhiroSuzuki***
Effects Copper Phosphus on Temper Embrittlement Mn-Mo-Ni Low Alloy Steel (ASTM )* ByMasayoshiHasegawa**, NobuyaNakajima***, NobuharuKusunoki*** KazuhiroSuzuki*** effect copper on temper steel. After beg
More informationá61ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: MICROBIAL ENUMERATION TESTS
USP 40 Microbiological Tests / á61ñ Microbiological Examination 1 á61ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: MICROBIAL ENUMERATION TESTS INTRODUCTION The tests described hereafter will allow
More informationTransmission Electron Microscopic Study of Antibiotic Action on Klebsiella pneumoniae Biofilm
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Aug. 2002, p. 2679 2683 Vol. 46, No. 8 0066-4804/02/$04.00 0 DOI: 10.1128/AAC.46.8.2679 2683.2002 Copyright 2002, American Society for Microbiology. All Rights Reserved.
More informationcorrected to ph 6.5 with NaOH and then mixed with the remaining ingredients
INDUCED COLONIAL VARIATION OF A TOTAL POPULATION AMONG CERTAIN LACTOBACILLI MORRISON ROGOSA AND JOYCE A. MITCHELL National Institutes of Health, National Institute of Dental Research, Bethesda, Maryland
More informationAntifungal Susceptibility Testing of Dermatophytes by Agar Based Disk Diffusion Method
ISSN: 23-7706 Volume 4 Number 3 (2015) pp. 430-436 http://www.ijcmas.com Original Research Article Antifungal Susceptibility Testing of Dermatophytes by Agar Based Disk Diffusion Method R.K.Agarwal 1 *,
More informationLab 2-Microbial Enumeration
Lab 2-Microbial Enumeration 2/19/08 CE 573 Introduction There are many different techniques that can be utilized when trying to quantify microorganisms found in a given sample. The purpose of this lab
More informationSKIN INFECTION OF RABBITS WITH HEMOLYTIC STREP- TOCOCCI ISOLATED FROM A PATIENT WITH ERYSIPELAS.
SKIN INFECTION OF RABBITS WITH HEMOLYTIC STREP- TOCOCCI ISOLATED FROM A PATIENT WITH ERYSIPELAS. I. METHOD OF DEMONSTRATING PROTECTIVE ACTION OF IMMUNE SERA. BY THOMAS M. RIVERS, M.D. (From the Hospital
More informationFinal text for addition to The International Pharmacopoeia
March 2012 3.3.1 MICROBIOLOGICAL EXAMINATION OF NON-STERILE PRODUCTS: MICROBIAL ENUMERATION TESTS Final text for addition to The International Pharmacopoeia This monograph was adopted at the Forty-sixth
More informationA MICROBIAL RESISTANCE EVALUATION OF INDOOR MATERIALS AIR KRETE INSULATION SAMPLE. prepared for AIR KRETE
A MICROBIAL RESISTANCE EVALUATION OF INDOOR MATERIALS AIR KRETE INSULATION SAMPLE prepared for AIR KRETE May 16, 2003 AQS Report No. 10681-02 TABLE OF CONTENTS Page Number Executive Summary Project Description...
More informationá62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS
USP 40 Microbiological Tests / á62ñ Microbiological Examination 1 á62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS INTRODUCTION The tests described hereafter
More informationTest Report. The Report of Acute Oral Toxicity Test
Test Report Sample No.: 2011KF0435 Test Item: Safe Nucleic Acid Stain Sponsor: Yekta Tajhiz Azma The Report of Acute Oral Toxicity Test Test item: Safe Nucleic Acid Stain Sample No.: 2011KF0435 Subject
More informationBacterial Counts - Quantitative Analysis of Microbes
Bacterial Counts - Quantitative Analysis of Microbes Introduction: It is often important to know not only what types of bacteria are in a sample but also how many of them are present. Food manufacturers
More informationMechanisms of Inhibition of Fungi in Agar by Streptomycetes
J. gen. Microbiol. (196g), 57, 19-8 With I plate Printed in Great Britain 1 9 Mechanisms of Inhibition of Fungi in Agar by Streptomycetes By s. c. HSU AND J. L. LOCKWOOD Department of Botany and Plant
More informationPURE CULTURE TECHNIQUES
PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,
More informationSuggest a technique that could be used to provide molecular evidence that all English Elm trees form a clone. ... [1]
1 Molecular evidence E Ulmus procera, form a genetically isolated clone. English Elms developed from a variety of elm brought to Britain from Rome in the first century A.D. Although English Elm trees make
More informationDRUG ANTIBIOTIC INTERACTIONS-ANTIMALARIALS
DRUG ANTIBIOTIC INTERACTIONS-ANTIMALARIALS Abstract Pages with reference to book, From 37 To 40 Najma Sultana ( Department of Pharmaceutical Chemistry, University of Karachi, Karachi-32. ) M. Saeed Arayne
More informationAseptic Techniques. A. Objectives. B. Before coming to lab
Aseptic Techniques A. Objectives Become familiar with 1. The ubiquity of microorganisms (see Note 1) 2. Aseptic techniques (see Note 2) 3. Standard methods for growing/observing microorganisms (see Note
More informationLab Exercise #4 Microbial Control Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic
Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic I. OBJECTIVES: Investigate the effectiveness various agents of control. Assess the effectiveness of heat in killing vegetative
More informationPreliminary Evaluation of a Semisolid Agar Antifungal Susceptibility Test for Yeasts and Molds
JOURNAL OF CLINICAL MICROBIOLOGY, Feb. 2000, p. 537 541 Vol. 38, No. 2 0095-1137/00/$04.00 0 Copyright 2000, American Society for Microbiology. All Rights Reserved. Preliminary Evaluation of a Semisolid
More informationDr: RAWIA BADR Associate Professor of Microbiology&Immunology
Dr: RAWIA BADR Associate Professor of Microbiology&Immunology Cell culture Commonly refers to the culture of animal cells and tissues, while the more specific term plant tissue.culture is used only for
More informationCo-Existence of Photosynthetic Bacteria, Streptomyces and Lactic Acid Bacteria in Solutions of Effective Microorganisms Abstract Introduction
Co-Existence of Photosynthetic Bacteria, Streptomyces and Lactic Acid Bacteria in Solutions of Effective Microorganisms Masanobu Sakurai EM Laboratory, International Nature Farming Research Center, Shizuoka,
More informationA Simple and Reliable Assimilation Test for the Identification of Candida Species
Simple and Reliable ssimilation Test for the Identification of Candida Species MRION V. MRTIN, M.D., ND J. D. SCHNEIDU, JR., PH.D. Department of Microbiology and Immunology, Tulane University School of
More informationIsolation & Characterization of Bacteria
PR025 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Isolation & Characterization of Bacteria Teacher s Handbook (Cat. # BE 204) think proteins!
More informationMidori Green DNA Stain Safety Test Reports
Midori Green DNA Stain Safety Test Reports IDENTIFICATION OF THE PRODUCT AND OF THE COMPANY Product name Catalog number Supplier Information in case of emergency Midori Green DNA Stain MG01 MG02 Nippon
More informationHiPer Transformation Teaching Kit
HiPer Transformation Teaching Kit Product Code: HTBM017 Number of experiments that can be performed: 10 Duration of Experiment: 4 days Day 1- Preparation of media and revival of E. coli Host Day 2- Inoculation
More informationBiocide Resistance in E.coli
BIO 370 1 Biocide Resistance in E.coli The ideal lab in an Evolutionary Biology class would be one where we demonstrate natural selection in response to a selection pressure. This is easy enough to do
More informationTipaporn Subsomboon 1*, Pittaya Liewseree 2, Sukum Kositchaimongkol 3 Received: 25 January 2012 ; Accepted: 11 April 2012
Original Tipaporn Subsomboon 1*, Pittaya Liewseree 2, Sukum Kositchaimongkol 3 Received: 25 January 2012 ; Accepted: 11 April 2012 Abstract An investigation of media suitable for growth and development
More informationIdentification of the G143A Mutation in Cytochrome b Associated with QoI Resistance in Cercospora beticola Isolates from the Red River Valley
2013. Plant Management Network. This article is in the public domain. Accepted for publication 31 May 2013. Published. Identification of the G143A Mutation in Cytochrome b Associated with QoI Resistance
More informationGrowth of Allescheria boydii in Antibiotic- Containing Media
JOURNAL OF BATERIOLOGY, Nov., 1965 opyright 1965 American Society for Microbiology Vol. 90, No. 5 Printed in U.S.A. Growth of Allescheria boydii in Antibiotic- ontaining Media JOHN AZIN, JR., AND DAVID
More informationMICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods
Issue No.29 / March 2010 MICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods By Dr Vincent TURBLIN, Deputy Regional Market Manager Poultry - CEVA Animal Health Asia Pacific Most
More informationJournal of Chemical and Pharmaceutical Research, 2013, 5(6):7-11. Research Article. Effect of copper sulphate on Citrobacter
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2013, 5(6):7-11 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Effect of copper sulphate on Citrobacter Srashti Dwivedi
More informationChapter 9 Antimicrobial Susceptibility Testing (Agar Disk Diffusion Method)
Chapter 9 Antimicrobial Susceptibility Testing (Agar Disk Diffusion Method) The disk diffusion method presented in this chapter has been carefully standardized by the National Committee for Clinical Laboratory
More informationBiofilm Protocol Optimization For Pseudomonas aeruginosa. Introduction. Materials and Methods. Culture Media, Incubation Time, and Biofilm Measurement
Biofilm Protocol Optimization For Pseudomonas aeruginosa Culture Media, Incubation Time, and Biofilm Measurement Introduction In addition to the conventional arsenal of antibiotic resistance mechanisms
More informationINTRODUCTION Contaminated serial dilution countable plates
INTRODUCTION In recent days, the foods that we consume are usually pre-processed in a facility removed from our home, cities, countries, and even continents. It is now more than ever important to be aware
More informationAntibiofilm Efficacy of Advanced Formula Zymox Otic Plus with 1% Hydrocortisone
Antibiofilm Efficacy of Advanced Formula Zymox Otic lus with 1% Hydrocortisone Rajvinder Atwal, hd¹ Anim Omar, h.d., B. harma² Merle Oson, DVM, MSc.² Nicole Butler, B.Sc.² Yanira Caberra, B.Sc.² ¹Laclede
More informationChapter - 9 IN VITRO CYTOTOXICITY ASSAY OF ZERUMBONE AND MDM3:1
Chapter - 9 IN VITRO CYTOTOXICITY ASSAY OF ZERUMBONE AND MDM3:1 9.1 INTRODUCTION 9.2 CHAPTER OBJECTIVE 9.3 MATERIALS AND METHODS 9.4 RESULT 9.5 DISCUSSION In Vitro Cytotoxicity Assay of Zerumbone and MDM
More informationELISA for Alpha-hemolysin (Hla) in Methicilin-resistant Staphylococcus aureus (MRSA) Varandt Y. Khodaverdian 1* and Menachem Shoham 2
ELISA for Alpha-hemolysin (Hla) in Methicilin-resistant Staphylococcus aureus (MRSA) Varandt Y. Khodaverdian 1* and Menachem Shoham 2 1 Department of Biology, Tufts University, Medford, USA; 2 Department
More informationGenetic Engineering: Transforming Bacteria
Genetic Engineering: Transforming Bacteria Introduction Activity Introduction In this laboratory experiment, students will transform the phenotype of bacteria through the use of genetic engineering. A
More informationTransport of Bacteria on Sloping Soil Surfaces by Runoff
Transport of Bacteria on Sloping Soil Surfaces by Runoff Jamal Abu-Ashour, 1 Hung Lee 2 1 Department of Agricultural Engineering and Technology, Jordan University of Science and Technology, Irbid 22110,
More informationSection IX: Special Applications in Agarose Gels
Section IX: In This Section Amplification of Plasmid cdna Libraries with SeaPrep Agarose 150 Preparing Agarose for use in Cell Culture Applications 152 References 154 149 Section IX: Amplification of Plasmid
More informationMicrobial Separation from a Complex Matrix by a Hand-Held Microfluidic Device
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Supplementary Information Microbial Separation from a Complex Matrix by a Hand-Held Microfluidic
More informationMICROBIAL GROWTH. Dr. Hala Al-Daghistani
MICROBIAL GROWTH Dr. Hala Al-Daghistani Microbial Growth Microbial growth: Increase in cell number, not cell size! Physical Requirements for Growth: Temperature Minimum growth temperature Optimum growth
More informationTACS MTT Assays. Cell Proliferation and Viability Assays. Catalog Number: TA tests. Catalog Number: TA tests
TACS MTT Assays Cell Proliferation and Viability Assays Catalog Number: TA5355-2500 tests Catalog Number: TA5412-5000 tests This package insert must be read in its entirety before using this product. FOR
More informationThe Production of a Recombinant Biotechnology Product. Chapter 8
The Production of a Recombinant Biotechnology Product Chapter 8 Objectives Give a basic overview of genetic engineering. Describe the processes involved in isolating a piece DNA of interest Mass producing
More informationHiPer Yeast Genomic DNA Extraction Teaching Kit
HiPer Yeast Genomic DNA Extraction Teaching Kit Product Code: HTBM013 Number of experiments that can be performed: 10 Duration of Experiment: 3 days Day 1: Revival of Host Day 2: Inoculation of culture
More informationPelagia Research Library. Effect of metal ions and drugs on antibacterial activities of Nigella sativa (L.) seeds
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2011, 1 (2):41-46 ISSN: 2248 9215 Effect of metal ions and drugs on antibacterial activities of Nigella sativa
More informationEZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK
EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 8 Rev 05/0/03 EZ-0 Genomic DNA Kit Handbook Table of Contents Introduction Limitations of Use Features Applications
More informationLab Exercise 13: Growth Curve
Lab Exercise 13: Growth Curve OBJECTIVES 1. Know the different phases of a standard growth curve. 2. Understand and perform direct measurement of bacterial growth through serial dilutions and standard
More informationHiPer Plasmid DNA Cloning Teaching Kit
HiPer Plasmid DNA Cloning Teaching Kit Product Code: HTBM022 Number of experiments that can be performed: 5 Duration of Experiment: 4 days Day 1- Preparation of media and revival of E. coli Host Day2-
More informationEmpfindlichkeitstestung bei Pilzen Neuigkeiten? Bericht aus einem EUCAST AFST (yeasts and moulds) Netzwerk-Laboratorium
Empfindlichkeitstestung bei Pilzen Neuigkeiten? Bericht aus einem EUCAST AFST (yeasts and moulds) Netzwerk-Laboratorium EUCAST reloaded 6.0 Follow-up Workshop 23.03.2017 Cornelia Lass-Flörl Division of
More informationOrthophenylphenol in healthcare environments: a trial related to a new administration method and a review of the literature*
Turkish Journal of Medical Sciences http://journals.tubitak.gov.tr/medical/ Research Article Turk J Med Sci (2013) 43: 805-809 TÜBİTAK doi:10.3906/sag-1208-4 Orthophenylphenol in healthcare environments:
More informationPROTOCOL 1: EXTRACTION OF DNA FROM BACTERIA
PROTOCOL 1: EXTRACTION OF DNA FROM BACTERIA The basic standard procedures for isolation of bacterial DNA are based on lysozyme digestion of the cell wall, detergent lysis, disruption of protein-nucleic
More informationRecrystallization II 23
Recrystallization II 23 Chem 355 Jasperse RECRYSTALLIZATIN-Week 2 1. Mixed Recrystallization of Acetanilide 2. Mixed Recrystallization of Dibenzylacetone 3. Recrystallization of an Unknown Background Review:
More informationDetecting Bacteria and Determining Their Susceptibility to Antibiotics by Stochastic Confinement in Nanoliter Droplets Using Plug-based Microfluidics
Supplemental Material for Detecting Bacteria and Determining Their Susceptibility to Antibiotics by Stochastic Confinement in Nanoliter Droplets Using Plug-based Microfluidics James Q. Boedicker, Liang
More informationDepartment of Microbiology, Mt. Sinai Hospital and University Health Network,
JCM Accepted Manuscript Posted Online 22 November 2017 J. Clin. Microbiol. doi:10.1128/jcm.01481-17 Copyright 2017 Gandhi et al. This is an open-access article distributed under the terms of the Creative
More informationINTRODUCTION Sanitization sterilization Antibiotics Bactericidal Bacteriostatic Antiseptics disinfectants
INTRODUCTION Infectious agents on environmental surfaces, given the correct circumstances, may potentially find their way into an unsuspecting victim. Thus, it is important to keep the surfaces we regularly
More informationJournal of Chemical and Pharmaceutical Research, 2014, 6(5): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 14, 6(5):7-33 Research Article ISSN : 975-7384 CODEN(USA) : JCPRC5 Impact of de-aeration methods on the culture of Clostridium
More informationACCEPTED. Yanjun Li 1. M. Hong Nguyen 2,3,4. Harmut Derendorf 1. Shaoji Cheng 2. *Cornelius J. Clancy 2,3
AAC Accepts, published online ahead of print on 21 May 2007 Antimicrob. Agents Chemother. doi:10.1128/aac.00308-07 Copyright 2007, American Society for Microbiology and/or the Listed Authors/Institutions.
More informationINTRODUCTION water-soluble Figure 1.
INTRODUCTION Natural waters contain bacteria. The aerobic gram negative bacillus of the genera Psedomonas, Alcalignes, and Flavobacterium are common in natural waters. Many of these bacteria are able to
More information2. Crystallization. A. Background
2. Crystallization A. Background Crystallization is one of several available techniques available to purify organic compounds. Unlike other techniques, however, crystallization is specific to the purification
More informationBACTERIAL CONJUGATION. To demonstrate the technical procedure to monitor the conjugational transfer of genetic material from one cell to another.
BACTERIAL CONJUGATION I. OBJECTIVES To demonstrate the technical procedure to monitor the conjugational transfer of genetic material from one cell to another. To learn about the various genetic elements
More informationChapter 3. Minimal inhibitory concentration (MIC) test and determination of antimicrobial resistant bacteria
Chapter 3. Minimal inhibitory concentration (MIC) test and determination of antimicrobial resistant bacteria Ruangpan, Lila Date published: 2004 To cite this document : Ruangpan, L. (2004). Chapter 3.
More informationABC. Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Volume 19 Number 18
M26-A ISBN 1-56238-384-1 September 1999 ISSN 0273-3099 Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline Volume 19 Number 18 Arthur L. Barry, Ph.D. William A. Craig,
More informationConfirming the Phenotypes of E. coli Strains
Confirming the Phenotypes of E. coli Strains INTRODUCTION Before undertaking any experiments, we need to confirm that the phenotypes of the E. coli strains we intend to use in the planned experiments correspond
More informationMeasuring, sampling, and analyzing white waters
T 656 cm-97 TENTATIVE STANDARD 1930 OFFICIAL STANDARD 1931 REVISED 1940 CORRECTED 1961 CLASSICAL METHOD 1983 REVISED 1997 1997 TAPPI The infmation and data contained in this document were prepared by a
More informationProtocol. Micronucleus Assay. with
with Page 2 of 8 Content 1. Background 3 2. Basic Procedure 3 3. Materials 4 3.1. epics kit components 4 3.2. Additionally needed materials and laboratory equipment 4 4. Method 5 4.1. Tissue and Medium
More informationBACTERIAL BIOFILMS FORMATION AT AIR LIQUID INTERFACES
Innovative Romanian Food Biotechnology Vol. 5, Issue of December, 009 009 by Dunărea de Jos University Galaţi Received September 1, 009/ Accepted November 8, 009 RESEARCH ARTICLE BACTERIAL BIOFILMS FORMATION
More informationextremely different from terrestrial conditions, it is supposed that microorganisms in
456 THE JOURNAL OF ANTIBIOTICS AUG. 1972 STUDIES ON MARINE MICROORGANISMS. I ISOLATION FROM THE JAPAN SEA Yoshiro Okami and Takao Okazaki Institute of Microbial Chemistry, Shinagawa-ku, Tokyo, Japan (Received
More informationProject 7: Wound Cultures and Identification
Project 7: Wound Cultures and Identification Readings: https://labtestsonline.org/understanding/analytes/wound-culture/tab/test Identification of Gram-Positive & Gram-Negative Bacteria Guide to laboratory
More informationSANIDATE 5.0 -BLUEBERRY STUDY
22 Meadow Street East Hartford, CT 06108 Phone: 860.290.8890 Fax: 860.290.8802 Solutions@BioSafeSystems.com 1-877-358-1299 www.biosafesystems.com www.enviroselects.com SANIDATE 5.0 -BLUEBERRY STUDY PI:
More informationINTERACTIONS OF ORAL STRAINS OF CANDIDA ALBICANS
INTERACTIONS OF ORAL STRAINS OF CANDIDA ALBICANS AND LACTOBACILLI GENEVIEVE YOUNG, R. I. KRASNER, AND P. L. YUDKOFSKY Department of Biology, Boston University, Boston, Massachusetts Received for publication
More informationEM021. Co-Trimoxazole Ezy MIC TM Strip (COT)( mcg/ml) (Trimethoprim/ Sulphamethoxazole) Antimicrobial Susceptibility Testing
Co-Trimoxazole Ezy MIC TM Strip (COT)(0.002-32 mcg/ml) (Trimethoprim/ Sulphamethoxazole) Antimicrobial Susceptibility Testing For In Vitro Diagnostic use EM021 Not for Medicinal Use It is a unique MIC
More informationKILL-TIME STUDIES Antimicrobial Activity of EO H 2 O Using Clostridium difficile spores Test Solutions: Acidic H 2 O Submitted December 12, 2011
KILL-TIME STUDIES Antimicrobial Activity of EO H 2 O Using Clostridium difficile spores Test Solutions: Acidic H 2 O Submitted December 12, 2011 December 20, 2011 PREPARED FOR: Rob Dahl Viritec, LLC. BY:
More informationnanoprecipitation mpeg-pla 2 nd Emulsion mpeg-pla in DCM
THERAPEUTIC NANOTECHNOLOGY LAB MODULE Location: BioNano Lab, 3119 Micro and Nanotechnology Laboratory (MNTL) Instructor: Jianjun Cheng, Assistant Professor of Materials Science and Engineering Lab Assistants:
More informationDr. Rukumani Devi Velayuthan Mycology Unit Co-ordinator PPUM
Antifungal sensitivity testing using VITEK 2 Dr. Rukumani Devi Velayuthan Mycology Unit Co-ordinator PPUM VITEK 2 Systems The VITEK 2 System is intended for the automated identification and susceptibility
More informationOPTIMIZATION OF MICROESCLEROTIA PRODUCTION BY TRICHODERMA ASPERELLUM
OPTIMIZATION OF MICROESCLEROTIA PRODUCTION BY TRICHODERMA ASPERELLUM Gabriel O. Locatelli 1, Christine L. L. Finkler 2, Gabriel M. Mascarin 3, Murillo Lobo Junior 3, Luciano A. Bueno 4. 1 Federal Rural
More informationEffect of Bentonite Clay on the Growth of Gwumunnumyces gramhis var. tritici and on its Interactions with Antagonistic Bacteria
Journal of General Microbiology (1983), 129, 771-777. Printed in Great Britain 771 Effect of Bentonite Clay on the Growth of Gwumunnumyces gramhis var. tritici and on its Interactions with Antagonistic
More informationInoculation Technique, Infection Development and Efficacy of Fungicides Against Glomerella cingulata the Causal Agent of Camellia Dieback
- Inoculation Technique, Infection Development and Efficacy of Fungicides Against Glomerella cingulata the Causal Agent of Camellia Dieback A. D. Martinez-Espinoza Department of Plant Pathology - Griffin
More informationRAC. Interpretation Guide. Rapid Aerobic Count Plate
Interpretation Guide The 3M Petrifilm is a sample-ready-culture medium system which contains nutrients, a cold-water-soluble gelling agent and a dual-sensing indicator technology that facilitates aerobic
More informationBacterial Transformation and Protein Purification
Bacterial Transformation and Protein Purification Group 4 Natalie Beale Gregory A. Pate Justin Rousseau Dohee Won Introduction The purpose of this experiment is to perform a genetic transformation and
More informationPLP 6404 Epidemiology of Plant Diseases Spring 2015 LAB 2 PHASES IN THE DISEASE CYCLE: GREENHOUSE AND LAB EXERCISE
PLP 6404 Epidemiology of Plant Diseases Spring 2015 LAB 2 PHASES IN THE DISEASE CYCLE: GREENHOUSE AND LAB EXERCISE "Variation in Host-Pathogen Interactions and its Effect on Epidemic Development" Purpose:
More informationMechanisms of Protection in Ayu Orally Vaccinated for Vibriosis
Fish Pathology 15 (3/4) 257-262,1981. 3 Mechanisms Protection Ayu Orally Vaccated for Vibriosis Kenji KAWAI*, Riichi KUSUDA* and Toshiaki ITAMI* *Fish Dis. Lab., Fac. Agr., Kochi Univ., Nankoku, Kochi,
More informationSpostiamo ora la nostra attenzione sui batteri, e batteriofagi
Spostiamo ora la nostra attenzione sui batteri, e batteriofagi Bacteria Mutate Spontaneously and Grow at an Exponential Rate. Useful for genetics studies, development of genetic engineering Teoria dell'adattamento
More information