Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Microbiology Laboratory Contact: Emer Fahy Charing Cross Hospital Tel: +44 (0) Fulham Palace Road Website: London W6 8RF Testing performed at the above address only DETAIL OF ACCREDITATION TISSUES Blood, Bone Bronchio-alveolar lavages (BAL) / washings, CPAP fluid Contact lens and fluid Corneal scrape material CSF Drain fluid, dressings, bile Expressed breast milk Fluids, Aspirates, antral washouts EBUS) Pleural effusions / fluid Prostheses, heart valves, Intra uterine devices Semen Skin, hair, nails Sputum Swabs, pus Tips and lines Tissue, biopsies, brushes s, suprapubic aspirates, nephrostomy, ileal conduits, void bladder urines. Trichomonas vaginalis cultures clinical diagnosis General isolation for characterisation of microorganisms for the General isolation for characterisation of microorganisms for the Manual or automated inoculation, incubation and image capture of culture media using: CSF SOP (MIC-LP-006-X) Cross Infection (MIC-LP-003-X) ENT SOP (MIC-LP-008-X) MC&S SOP (MIC-LP-009-X) C.difficile culture (MIC-LP-005-X) Female genital SOP (MIC-LP-140-X) GUM Bench SOP (MIC-LP-141-X) Fungal Culture SOP (MIC-LP-159-X) Superficial Mycoses (MIC-LP-073-X) H.pylori Culture SOP (MIC-LP-011-X) Peritoneal Dialysis Fluid SOP (MIC-LP-012-X) Sterile fluids SOP (MIC-LP-013-X) Sterility Testing (MIC-LP-084-X) Respiratory SOP (MIC-LP-018-X) Tissue and Bone SOP (MIC-LP-014-X) Wounds & General Set Up Bench SOP (MIC- LP-137-IMP) Wounds and General bench reading SOP (MIC- LP-136-IMP) Kiestra SOP MIC-LP-163-X Acanthamoeba SOP (MIC-LP-007-X) GUM Bench SOP MIC-LP-141-X Wounds and General bench reading SOP (MIC- LP-136-IMP) Assessment Manager: DE Page 1 of 6
2 Blood, body fluids, bone marrow harvests, stem cells Sputum Bronchio-alveolar lavages (BAL) / washings, Pleural fluid / effusions CSF, Fluids Early Morning Gastric washings EBUS), Pus, Tissues, biopsies, brushes, skin biopsies, Blood, bone marrow Swabs, Bacteria, Fungi CSF, peritoneal dialysis fluid, ascitic fluid, Prosthetic joint fluid Screening for Methiicillin resistant Staphylococcus aureus (MRSA ) Antimicrobial susceptibility testing of clinically significant bacteria and fungi Semi-quantification of white blood cells, red blood cells, squamous epithelial cells, bacteria, yeast, casts, spermatazoa Quantification of white blood cells and red blood cells Fluorescent detection of CO2 levels to detect microorganisms using BACTEC FX: Blood culture FX (MIC-LP-021-X) Stem Cell SOP (MIC-LP-139-X) Mycobacterial methods (MIC-LP-017-X) Fluorescent Detection of O2 levels to detect Mycobacteria using Mycobacteria Growth Indicator Tube (MGIT), manual culture using Kirschner liquid medium Mycobacterial methods (MIC-LP-017-X) Manual or automated inoculation, incubation and image capture of culture media using: Cross Infection (MIC-LP-003-X) Manual disc diffusion using the latest EUCAST methodology. Manual reading or automated digital imaging by Kiestra using: EUCAST sensitivity testing (MIC-LP-044-X) Fungal Susceptibility Testing (MIC-LP-076-X) Kiestra SOP MIC-LP-163-X Manual microscopy (unstained) or automated cell counting by digital imaging using Sedimax: SOP Misys (MIC-LP-058-X) Menarini Sedimax SOP (MIC-LP-133-IMP), SOP Misys (MIC-LP-058-X) Manual count: CSF SOP MIC-LP-006-X, Peritoneal dialysis fluid SOP MIC-LP-012-X, Sterile fluids MIC-LP-013-X Assessment Manager: DE Page 2 of 6
3 Automated identification of bacteria, yeasts Identification of bacteria, yeasts and fungi Serological typing of Salmonella sp. PSO, PSH, Vi, Shigella sp., E. coli O157, V. cholera O1 Identification using Bruker Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF): MALDI-TOF SOP (MIC-LP-134-X) Identification and staining methods MIC-LP-047- IMP using the following manual techniques: Gram stain, Manual biochemical test assays. Using manual slide agglutination technique MC&S SOP MIC-LP-009-X Culture positive liquid media Identification of M. tuberculosis complex isolates Using BD MGIT MTBc identification card: Mycobacterial methods SOP MIC-LP-017-X Blood, Bone marrow, stem cell material, Bone Bronchio-alveolar lavages (BAL) / washings, Pleural effusions / fluid Contact lens and fluid Corneal scrape material CSF, Fluids, Aspirates, antral washouts, drain fluids EBUS) Pus, Sputum, Swabs Prostheses, heart valves Tissue, biopsies, brushes order to detect and differentiate microorganisms and cellular elements for the Light microscopy (stained) using Gram stain, Diff-Quick Differential stain and Lactophenol cotton blue, lactofuschin Microbiology Identification & Staining Methods (MIC-LP-047-IMP) Kiestra SOP MIC-LP-163-X CSF SOP (MIC-LP-006-X) Cross Infection (MIC-LP-003-X) ENT SOP (MIC-LP-008-X) MC&S SOP (MIC-LP-009-X) C.difficile culture (MIC-LP-005-X) Female genital SOP (MIC-LP-140-X) GUM Bench SOP (MIC-LP-141-X) Fungal Culture SOP (MIC-LP-159-X) Superficial Mycoses (MIC-LP-073-X) H.pylori Culture SOP (MIC-LP-011-X) Peritoneal Dialysis Fluid SOP (MIC-LP-012-X) Sterile fluids SOP (MIC-LP-013-X) Sterility Testing (MIC-LP-084-X) Respiratory SOP (MIC-LP-018-X) Tissue and Bone SOP (MIC-LP-014-X) Wounds & General Set Up Bench SOP (MIC- LP-137-IMP) Wounds and General bench reading SOP (MIC- LP-136-IMP) Assessment Manager: DE Page 3 of 6
4 Sputum, Bronchio-alveolar lavages (BAL) Pleural fluid / effusions CSF, Fluids, Pus Gastric washings EBUS), Tissues, biopsies, brushes, skin biopsies, Blood, bone marrow Skin, Hair, Nails Sputum, Bronchio-alveolar lavages (BAL) / washings, Pleural fluids / effusions Blood, bone marrow CSF, Fluids, Pus Tissues, biopsies, Sellotape slides, parasitic material order to detect and differentiate microorganisms and cellular elements for the order to detect the presence of parasites (ova, cysts, trophozoites, worms) order to detect the presence of Schistosomes. order to detect the presence of Cryptosporidium sp. Antigen Detection Helicobacter sp. antigen GDH antigen Immunofluorescent microscopy using Auramine staining and Light microscopy using Ziehl Neelsen staining: Mycobacterial methods (MIC-LP-017-X) Immunofluorescent microscopy using Calcoflor and Light Microscopy (unstained Superficial Mycoses (MIC-LP-073-X), Long term (Systemic) Mycoses (MIC-LP-074-X), Short Term Fungal Culture (MIC-LP-075-X) Manual microscopy (unstained) OCP SOP (MIC-LP-010-X) Manual microscopy (unstained): SOP MIC-LP-058-X Immunofluorescent microscopy using auramine staining: OCP SOP (MIC-LP-010-X) Using the Dynex DS2 Automated ELISA System: Helicobacter Antigen SOP MIC-LP-028-X) DS2 SOP (MIC-LP-027-X) C.diff chek-60 GDH (MIC-LP-001-X), Assessment Manager: DE Page 4 of 6
5 Serum Detection of GDH and Clostridium difficile toxins A and B Detection of viral antigens Rota/Adenovirus antigen detection Norovirus antigen detection Rapid Detection of HIV-1/2 Antigen/Antibody Using rapid membrane enzyme immunoassay: C. Diff Quik Chek Complete C.difficile Rapid (MIC-LP-004-X) Using Manual Immunochromatographic lateralflow: RIDA Quick Rotavirus/Adenovirus Combi (N1002) MIC-LP-148-IMP RIDA Norovirus N1402 MIC-LP-149-X Using Determine HIV-1/2 Antigen/Antibody Combo: HIV-1/2 Antigen/Antibody Combo MIC-LP-161-X Serum, CSF Cryptococcal Antigen Using IMMY Lateral Flow Assay Cryptococcal Antigen Detection SOP (MIC-LP- 153-X) Pneumococcal Urinary Antigen Binax NOW Streptococcus pneumonia urinary antigen card: Pneumococcal Urinary Antigen (MIC-LP-022-X) Legionella Urinary Antigen Binax NOW Legionella urinary antigen card: Legionella Urinary Antigen (MIC-LP-023-X) Sputum, Bronchio-alveolar lavages (BAL) / washings, ultra sound guided EBUS) Molecular examination clinical diagnosis Detection of Tuberculosis by PCR In house documented procedures based on equipment manuals and standard methods: Using GeneXpert Infinity-48 real time PCR: TB GeneXpert Infinity SOP (MIC-LP-129-X) Swabs, Detection of MRSA by PCR Using GeneXpert Infinity-48 real time PCR: MRSA GeneXpert Infinity-48 RT-PCR SOP (MIC-LP-151-IMP) Assessment Manager: DE Page 5 of 6
6 Molecular examination In house documented procedures based on equipment manuals and standard methods: Swabs Urethral, vaginal, vulvo-vaginal self-taken, rectal, throat, eye, swabs, urine Detection of Carbapenem Resistant organisms Detection of Clostridium difficile toxin by PCR Clostridium dificile ribotyping Chlamydia detection GC and Chlamydia DNA screen by PCR Using Cepheid GeneXpert Infinity CRO GeneXpert Infinity-48 RT-PCR SOP MIC- LP-156-IMP Using GeneXpert Infinity-48 real time PCR: C. difficile Xpert PCR SOP (MIC-LP-024-X) Using G-Storm GS1 thermocycler, Electrophoresis gel tank: Clostridium difficile PCR ribotyping (MIC-LP-138-X) Using Becton Dickinson (BD) Viper, Chlamydia / GC SDA Viper SOP (MIC-LP-020- X) Chlamydia trachomatis confirmation by PCR Detection of Chlamydia trachomatis LGV strains Measurement of antibiotic levels for clinical diagnosis: Using Cepheid GeneXpert Infinity CT Confirmations, Cepheid SOP MIC-LP-146-X Using Roche Magnapure Compact (Nucleic Acid Extraction system) and Lightcycler 96 (Nucleic acid amplification). Detection of Chlamydia trachomatis LGV strains (MIC-LP-157-IMP) Antibiotic Assays using Abbot Architect using Chemiluminescent Magnetic Immunoassay: Serum, Plasma Vancomycin CHM-ED-048-IMP, CHM-LP-101-IMP Gentamicin Amikacin Tobramycin CHM-ED-024-IMP, CHM-LP-101-IMP CHM-ED-011-IMP, CHM-LP-101-IMP CHM-ED-039-IMP, CHM-LP-101-IMP END Assessment Manager: DE Page 6 of 6
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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