Chemical Indicators. Biological Indicators. Physical Monitors. Welcome! How do I get a CE Certificate? Learning Objectives
|
|
- Daniela Miranda Thompson
- 5 years ago
- Views:
Transcription
1 3M Infection Prevention Solutions 3M Sterile U Web Meeting July 17, 2014 The Science of Speed The Evolution of Biological Indicators Welcome! Topic: The Science of Speed The Evolution of Biological Indicators Facilitators: Christophe de Campeau, 3M Sandra Velte, 3M Speakers: Craig Wallace Senior Technical Specialist 3M Housekeeping Questions Today s meeting times are 9:00 a.m., 11:00 a.m. and 1:00 p.m. CST How do I get a CE Certificate? difficulties Craig Wallace 3M follow-up For22 more information: Learning Objectives Next week, all of today s meeting participants will be sent an containing instructions for obtaining a CE Certificate for today s meeting. 1. Describe the design and function of biological indicators 2. Discuss how biological indicator incubation time is determined The will be sent to the address you provided when you logged-in to today s meeting. If there are others listening with you today who did not log-on, you may forward the CE certificate to them. 3 Chat feature Post session 2 3 Mute feature (*7 = unmute; *6 = mute) Technical To hear audio, call and enter access code Phone lines are muted. Audio will commence when the webinar begins. 3 Sterilization Monitoring - Quality Control 3. Understand how rapid readout biological indicator systems work 4 Sterilization Process Monitoring Tools Load Control Biological Indicators Pack Control Equipment Control 5 Chemical Indicators Physical Monitors Exposure Control 6 1
2 The history of biological indicators Late 1800s First biological indicators... National Institutes of Health (NIH) 1955 Robert Koch 1881 Recommends Bacillus stearothermophilus* as the test organism for steam sterilization Forms spores - challenges process Inoculated carriers No threat of pathogenicity, pyrogenicity, or toxicity * Now known as Geobacillus stearothermophilus 9 10 The basic principle of biological indicators BI Basics.... Direct measurement of lethality Large number of test organisms Test organisms that are very resistant to the process (hard to kill) If the process can kill the BI, it will kill the lower numbers of less resistant organisms on the medical devices
3 Bacterial Spores Bacterial Spores Some types of bacteria can form spores to protect themselves from a hostile environment, such as lack of nutrients e.g. some Bacillus sp., Clostridium sp. Protective shell around DNA, almost no water No biological activity - dormant Geobacillus stearothermophilus Bacillus atropheus 13 Google Images 14 Bacterial Spores - Formation Bacterial Spores Activation and germination Many complex steps driven by enzymes Google Images Google Images Bacterial Growth Bacterial Growth If we had a starting count of 10 contaminating bacteria that can grow and double every 20 minutes, you will have: Types of BIs Inoculated devices Elapsed Time # of Bacteria 0: : : : : : : :20 1,440 2:40 2,880 3:00 5,760 3:20 11,520 3:40 23,040 4:00 46,080 4:20 92,160 4:40 184,320 5:00 368,640 5:20 737,280 5:40 1,474,560 6:00 2,949, Cycle time: 20 minutes - 3 hours 18 3
4 Types of BIs Spore Strips Types of BIs Spore Ampoules Types of BIs Self-contained (SCBI) Self-contained BIs color change Growth of the test organisms produces acid ph of the growth media drops Cap Filter Growth Media with ph Indicator Spore Strip Sleeve ph indicator changes color positive BI Negative Positive Biological indicator testing - resistometer Biological indicator performance requirements ANSI/AAMI/ISO series
5 Key performance parameters Population (number of test organisms) Number of organisms that must be killed to get a negative BI The more organisms, the longer it takes to inactive the BI Measured by distributing the spores on plates and counting Expressed in exponents (e.g = 1,000,000) D-value (resistance) A measure of how resistant the organisms are to the process (how hard to kill) Measured as the time to reduce the population by 90% Expressed in time units (usually minutes) Biological indicators key specifications Moist heat D-value: 1.5 minute minimum (121 C) Population: 1 x 10 5 CFU (minimum) Ethylene oxide D-value: 2.5 minutes minimum Population: 1 x 10 6 CFU minimum ISO 11138: The evolution of BI technology BI evolution Consistency Purified cultures of carefully selected test organisms Convenience Self-contained designs eliminate transfers to growth media Biological indicator readout time Readout (or incubation) time: Time required to decide that a biological indicator is NEGATIVE 7 days is the accepted readout time for BIs for established sterilization processes, with known test organisms Readout time 7 days reduced to minutes ISO/ANSI/AAMI :
6 Reduced incubation time (RIT) A BI readout time of less than 7 days, which has been validated to an appropriate level of accuracy as compared to the 7 day result. FDA Guidance Reduced Incubation Time How is that done? standard seven or more days more than 97% Evolution of readout times????? Rapid biological indicator technologies* 7 days hours 1-4 hours minutes *Less than 24 hours Bacterial Growth Bacterial Growth If we had a starting count of 10 contaminating bacteria that can grow and double every 20 minutes, you will have: Elapsed Time # of Bacteria 0: : : : : : : :20 1,440 2:40 2,880 3:00 5,760 3:20 11,520 3:40 23,040 4:00 46,080 4:20 92,160 4:40 184,320 5:00 368,640 5:20 737,280 5:40 1,474,560 6:00 2,949,120 Mesa Labs Smart-Read Technology Standard SCBI growth media color change Colorimeter reads color shift and declares a positive when certain color change criteria are met Readout time is 10 hours Cycle time: 20 minutes - 3 hours Smart-Read is a registered trademark of Mesa Labs, Inc
7 Bacterial spores activation and germination Spore start up... Computer start up Many complex steps driven by enzymes Google Images Technology: 3M Rapid Attest and Super Rapid Attest Technology 3M Rapid Attest and Super Rapid Attest SCBIs Detects biological activity very early in the spore s activation and germination process Takes advantage of the activity of enzymes used by the cell to germinate and grow Detects the end product of the enzyme reactions Super Rapid Attest Cap Growth Media with ph Indicator and fluorescent indicator Spore carrier Sleeve Glucosidase enzymes - carbohydrate metabolism Geobacillus stearothermophilus: alpha-glucosidase Bacillus atrophaeus: beta-glucosidase Rapid Attest M Rapid Readout Technology 3M Rapid Readout Technology Non-fluorescent substrate in growth media Non-fluorescent substrate in growth media Fluorescent by-product (4-MU) detected in Auto-reader Fluorescent by-product (4-MU) detected in Auto-reader
8 Readout Times Rapid Attest hours Rapid Attest and Super Rapid Attest also can provide a conventional outgrowthbased ph color change response Super Rapid Attest 1492V 1 hour Summary Biological indicators provide a direct microbial challenge (large number of spores) to the sterilization process, so they are the only sterilization indicator that provides a direct measure of lethality. Biological indicators have evolved in consistency, convenience, and speed (readout time). Different technologies have been developed to detect a earlier signals from the spores, allowing faster readout times Next Month s 3M Sterile U Webinar Questions? August 21, 2014: The Nuts and Bolts of Washer-Disinfectors
Biological Monitoring
Biological Monitoring Prof. Duygu PERÇİN, MD Department of Clinical Microbiology Erciyes University Faculty of Medicine, Kayseri-TURKEY duygu.percin@hotmail.com Learning Objectives The attandee will be
More informationWelcome! How do I get a CE Certificate?
M Sterile U Network M Sterile U Web Meeting November 0, 0 Today s meeting times: 9:00 a.m., :00 a.m., and :00 p.m. Central Standard Time To hear audio, call 800-97-00 and enter access code 7 Phone lines
More informationProduct. Profile. For Industrial Applications
3M TM Attest TM 1294-S Rapid Readout Biological Indicator for Ethylene Oxide 3M TM Attest TM Auto-readers Product Profile For Industrial Applications Table of Contents Introduction... 3 Product Descriptions...
More informationSTERILIZATION VALIDATION IN THIS SECTION REPROCESSING VALIDATIONS FOR REUSABLE MEDICAL DEVICES. Radiation Sterilization Validation
STERILIZATION VALIDATION All sterilization processes require validation of the efficacy and reproducibility of the process. Depending on the type of sterilization, this may be accomplished by partial,
More informationIn the Know about.appropriate Use of 3M Attest Rapid Readout Biological Indicators
3M Attest Sterile U Web Meeting Jan 22, 2009 In the Know about.appropriate Use of 3M Attest Rapid Readout Biological Indicators ww.3m.com/atteststerileuonline 7/15 Modified per Inma s input. SDF Prepared
More informationThey can be used on washer-disinfectors, ultrasonic cleaners, surgical instruments and endoscopes. Page 5
Rev. 0 October 2017 Terragene offers a wide range of simple and effective products to precisely control the washing, hygiene and sterilization processes in veterinary clinics. Available in a variety of
More information8/13/2013. Keys to Facilities Planning. 3M Sterile U Network 3M Sterile U Web Meeting August 15th, 2013
3M Sterile U Network 3M Sterile U Web Meeting August 15th, 2013 Keys to Facilities Planning Today s meeting times: 9:00 a.m., 11:00 a.m. and 1:00 p.m. CST To hear audio, call 800-937-0042 and enter access
More informationSecure. Tested. Certified.
Secure. Tested. Certified. We solve problems with confidence and creativity. Monitoring Products for Dry Heat Steam-Formaldehyde FORM Chlorine Dioxide Table of Contents Crosstex Industrial is a division
More informationInstructions for Validation of Autoclaves
Instructions for Validation of Autoclaves A. Validation of autoclaves may accomplished by the following methods: 1. Autoclave tape applied to the outside of all packs to validate that the correct temperature
More informationPRODUCT CATALOGUE. for gke Steri-Record Biological Indicators, Process Challenge Devices and Accessories
PRODUCT CATALOGUE for gke Steri-Record Biological Indicators, Process Challenge Devices and Accessories Spore strips, Ampoules, Suspensions, Self-contained Mini-Bio-Plus Indicators to monitor steam, formaldehyde,
More informationHygiene Monitoring. Biological Indicators for Monitoring Sterilization Processes. Product Information. BI_07_en
Hygiene Monitoring Biological Indicators for Monitoring Sterilization Processes Product Information BI_07_en BAG-BioStrip Biological Indicators for Monitoring all Types of Sterilization Processes STEAM
More informationsterilisation monitoring products
sterilisation monitoring products Known for their consistent, reliable performance, Excelsior Scientific sterilisation monitoring products are used by pharmaceutical and medical device manufacturers, contract
More informationPRODUCT CATALOGUE. for gke Steri-Record Biological Indicators, Process Challenge Devices and Accessories
PRODUCT CATALOGUE for gke Steri-Record Biological Indicators, Process Challenge Devices and Accessories Spore strips, Ampoules, Suspensions, Self-contained Mini-Bio-Plus Indicators to monitor steam, formaldehyde,
More informationEH&S. Sheet. Fact. Safe and Effective Use of Autoclaves. What are autoclaves? Factors for effective sterilization. Dry heat cycle - when to use
Please post or circulate Fact heet nvironment, ealth and afety Information for the Berkeley Campus No. 33 Revised 04/04/11 afe and ffective Use of Autoclaves Autoclaves are easy to use but can pose a safety
More informationInnovative. Smart. Trusted.
Innovative. Smart. Trusted. We re your partner for sterilization products. Monitoring Products for Vaporized Hydrogen Peroxide Table of Contents Crosstex Industrial is a division of Crosstex International,
More informationEffect of Storage Conditions upon the Resistance (D-value) of Spore Strip Biological Indicators (BIs) used in EtO Sterilization Monitoring
Effect of Storage Conditions upon the Resistance (D-value) of Spore Strip Biological Indicators (BIs) used in EtO Sterilization Monitoring Wendy Royalty*, Mia Ware, Russell Nyberg Raven Biological Laboratories,
More informationISO INTERNATIONAL STANDARD
INTERNATIONAL STANDARD ISO 14161 Second edition 2009-09-15 Sterilization of health care products Biological indicators Guidance for the selection, use and interpretation of results Stérilisation des produits
More informationProduct Catalogue. for gke Steri-Record Biological Indicators, Process Challenge Devices and Accessories
Product Catalogue for gke Steri-Record Biological Indicators, Process Challenge Devices and Accessories Spore Strips, Ampoules, Suspensions, Self-contained Mini-Bio-Plus Indicators to monitor steam, formaldehyde,
More informationBest Practice Monitoring for Sterile Processing Professionals
Best Practice Monitoring for Sterile Processing Professionals Sterile processing departments are faced with greater challenges than ever. Increasing demands for quick turnarounds. Pressure to streamline
More information2018 North America Lab Services Fee Schedule
Product Bioburden - ISO 11737-1 Bioburden sample pooling, sample item portion (SIP) and additional material fees may apply as required by the sample type. BIO110 Bioburden: Aerobic bacteria only per unit
More informationLab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab
Lab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab OBJECTIVES 1. Understand the use of MPN to determine likely fecal water contamination. 2. Understand the use of MUG,
More informationINTRODUCTION Sanitization sterilization Antibiotics Bactericidal Bacteriostatic Antiseptics disinfectants
INTRODUCTION Infectious agents on environmental surfaces, given the correct circumstances, may potentially find their way into an unsuspecting victim. Thus, it is important to keep the surfaces we regularly
More informationISO INTERNATIONAL STANDARD
INTERNATIONAL STANDARD ISO 14161 First edition 2000-10-01 Sterilization of health care products Biological indicators Guidance for the selection, use and interpretation of results Stérilisation des produits
More informationCERTIFICATE OF CONFORMANCE Mesa Laboratories, Inc. 625 Zoot Way, Bozeman, MT Phone: (303)
Expiration : Bowie-Dick Test Sheets 77-05-CI-BD110 The ProChem Lead-Free Bowie-Dick Test Sheet is for daily monitoring of pre-vacuum sterilizers operating at 134 C for 3.5 minutes. The test sheet is designed
More informationSOP BIO-006 USE OF AUTOCLAVE FOR STERILIZATION OF MATERIALS AND BIOLOGICAL WASTE
ENVIRONMENTAL AND EMERGENCY MANAGEMENT Environmental Health and Safety University Crossing Suite 140 Lowell MA 01854 http://www.uml.edu/eem/ SOP BIO-006 USE OF AUTOCLAVE FOR STERILIZATION OF MATERIALS
More informationMICROBIOLOGICAL DETECTION OF E. COLI WITH UNPARALLELED SENSITIVITY. RUG A novel beta-glucuronidase substrate
MICROBIOLOGICAL DETECTION OF E. COLI WITH UNPARALLELED SENSITIVITY RUG A novel beta-glucuronidase substrate E. coli detection Escherichia coli (E. coli) is a Gram negative bacterium that inhabits the intestines
More informationINTRODUCTION Contaminated serial dilution countable plates
INTRODUCTION In recent days, the foods that we consume are usually pre-processed in a facility removed from our home, cities, countries, and even continents. It is now more than ever important to be aware
More informationgke Steri-Record Testsets and PCDs for validation, process and batch monitoring of sterilization processes
gke Steri-Record Testsets and PCDs for validation, process and batch monitoring of sterilization processes Application Process Monitoring Systems (PMS) are process challenge devices that are used to test
More informationChapter 6: Microbial Growth
Chapter 6: Microbial Growth 1. Requirements for Growth 2. Culturing Microorganisms 3. Patterns of Microbial Growth 1. Requirements for Growth Factors that affect Microbial Growth Microbial growth depends
More informationYour Sterile Processing Partner. Duraholder IPS
Your Sterile Processing Partner Table of Contents Item Page Duraholder Case Study 17 AAMI ST79 Reference 20 Steam Sterilization Efficacy Testing 21 PRODUCT INDEX Product Page Standard Pouches DURA050/100
More information3M Products and Services for Medical Sterilization Processes. Full Line Catalog. Medical
3M Products and Services for Medical Sterilization Processes Full Line Catalog Medical 3M Medical Sterilization Specialties 3M Medical Sterilization Specialties is a global leader in the manufacture and
More informationSlide 1. Slide 2. Slide 3. Sponsored by Webber Training Inc. For more information contact Paul Webber
Slide 1 QUALITY ASSURANCE IN STERILIZATION Susan Hadfield Director, Sterile Processing Department Health Sciences Centre Winnipeg, Manitoba Hosted by Paul Webber (paul@webbertraining.com) A teleclass sponsored
More informationKevin Lorcheim Project Engineer
Kevin Lorcheim Project Engineer In order for any decontamination method to be effective, the following points must be satisfied The decontamination method must: Be able to kill the organism in question
More information( 주 ) 세현테크 TEL : FAX :
All products manufactured by Mesa are done so in accordance with current Quality System Regulations of the United States Food and Drug Administration and meet the standards established by USP and ISO 11138.
More informationCurrent Topics For Sterile Generic Drug Products
Current Topics For Sterile Generic Drug Products Marla Stevens-Riley, Ph.D. Team Leader Division of Microbiology for ANDA Review FDA/CDER GPHA CMC Workshop June 4, 2014 Disclaimer Opinions expressed in
More informationAnnex A2. Guidance on Process Validation Scheme for Aseptically Processed Products
Annex A2 Guidance on Process Validation Scheme for Aseptically Processed Products 1 Table of content 1 PURPOSE... 3 2 SCOPE... 3 3 GENERAL INFORMATION... 3 4 INFORMATION NEEDED FOR ASEPTIC PROCESSES VALIDATION...
More informationEfficacy Report Summarization for SoClean 2
Efficacy Report Summarization for SoClean 2 October 2017 SoClean Inc 36 Town Forest Road Oxford, Massachusetts 01540 USA Tel. 508-363-0418 Email info@soclean.com SoClean 2 is USA FDA Registered 3009534409
More informationClostridium difficile Update Dr. Michelle Alfa, Winnipeg Sponsored by ARJO
Clostridium difficile: Update Dr. Michelle Alfa Ph.D, FCCM Diagnostic Services of Manitoba St. Boniface General Hospital Site Hosted by Paul Webber paul@webbertraining.com Sponsored by www.arjo.com www.webbertraining.com
More informationEZ-Fluo System. For rapid detection of spoilage organisms in wine
Application Note EZ-Fluo System For rapid detection of spoilage organisms in wine Many beverage manufacturing processes are susceptible to spoilage organisms like yeast or bacteria contamination. Contamination
More informationVHP Sterilization and Prion Inactivation
WFHSS Workshop Opatija Croatia March 27 th 2009 VHP Sterilization and Prion Inactivation Dr. Georgia Alevizopoulou Clinical Specialist Eastern Europe, Middle East & Africa VHP Sterilization and Prion Inactivation
More informationREUSABLE MEDICAL DEVICE VALIDATION
All sterilization processes require validation of the efficacy and reproducibility of the process. Depending on the type of sterilization, this may be accomplished by partial, sub-lethal, or repetitive
More informationMedline Gemini Sterilization Wrap. Test Studies
Medline Gemini Sterilization Wrap Test Studies Steam Efficacy : Steam PreVac Steam Efficacy: Steam Gravity Shelf Life: 180 Days Plasma Efficacy: STERRAD 100NX Plasma Efficacy: STERRAD NX Plasma Efficacy:
More informationWHY DO THEY PUT MINT IN TOOTHPASTE? WOULD GARLIC BE BETTER?
Activity 4.22 Student Sheet WHY DO THEY PUT MINT IN TOOTHPASTE? WOULD GARLIC BE BETTER? Purpose To investigate the antibacterial properties of plants. To develop practical skills. YOU NEED Agar plate seeded
More informationResponses to Questions for SYSTEM 1E Process Monitoring and Validation Webinar
Responses to Questions for SYSTEM 1E Process Monitoring and Validation Webinar MONITORING THE SYSTEM 1E PROCESSOR Q: I want to confirm that there is no need for a Biological Indicator (BI) and that use
More informationRev. 0 October 2017 Terragene offers a range of simple and effective products to precisely control washing and sterilization processes of different instruments in tattoo and body piercing studios. Available
More informationHigh Pressure Pasteurization of meat products
High Pressure Pasteurization of meat products Ahmed Yousef Professor of Food Microbiology The Ohio State University Reciprocal Meat Conference Columbia Missouri June 18, 2003 Novel Processing Technologies
More informationSample for use a Guide Revised IDEXX COLILERT -18 TEST METHOD FOR THE SIMULTANEOUS DETECTION OF TOTAL COLIFORMS AND E.
IDEXX COLILERT -18 TEST METHOD FOR THE SIMULTANEOUS DETECTION OF TOTAL COLIFORMS AND E. COLI IN WATER 1 IDEXX COLILERT -18 TEST METHOD FOR THE SIMULTANEOUS DETECTION OF TOTAL COLIFORMS AND E. COLI IN WATER
More informationEffective Use of Autoclaves
Published on UC Davis Safety Services (https://safetyservices.ucdavis.edu) Effective Use of Autoclaves SafetyNet #: 26 Autoclave Use Autoclaves (steam sterilizers) are metal pressure vessels that are used
More informationLab Exercise 13: Growth Curve
Lab Exercise 13: Growth Curve OBJECTIVES 1. Know the different phases of a standard growth curve. 2. Understand and perform direct measurement of bacterial growth through serial dilutions and standard
More informationMICROBIAL GROWTH. Dr. Hala Al-Daghistani
MICROBIAL GROWTH Dr. Hala Al-Daghistani Microbial Growth Microbial growth: Increase in cell number, not cell size! Physical Requirements for Growth: Temperature Minimum growth temperature Optimum growth
More informationEffective Heat Sterilization in CO 2 Incubators
Effective Heat Sterilization in CO 2 Incubators Volume 4, Number 3 Key Words Key Words Class 100 air Contamination control Contamination elimination HEPA filtration Incubator Heat Sterilization White Paper
More informationTests to Support Sterility Claim. Imtiaz Ahmed
Tests to Support Sterility Claim Imtiaz Ahmed Sterile Product As per TGO 77, a sterile product must comply with the requirements of the following tests: Sterility Test Bacterial Endotoxins Test Appendix
More information6/28/2016. Control of Microbial Growth. Method. Terminology. Disinfectants and Antiseptics
Control of Microbial Growth Disinfectants and Antiseptics 1 Method Three approaches for the control of microbial growth Chemical Disinfectants and antiseptics Physical Heat Ultraviolet Irradiations Mechanical
More informationSterile Compounding elearning Course Curriculum 28 lessons with 33 hours of CE. Fundamentals of Sterile Compounding (8 lessons/8 hours CE)
Sterile Compounding elearning Course Curriculum 28 lessons with 33 hours of CE Fundamentals of Sterile Compounding (8 lessons/8 hours CE) The History of Compounding and USP Sterile Compounding Chapters
More informationIDEXX Summary. IDEXX Laboratories. Date: April Report Highlights:
IDEXX Summary 17A Topic: Title: Author: Beta Trial Study report comparing Legiolert* versus the standard method in Germany, described in Trinkwasserverordnung 2001 in der Fassung v. 14.12.2012 Anlage 5,
More informationAS/NZS 4187 RELEVANT STANDARDS. Robyn Williams NUM CSSD LBHHS & Standards Australia Chair HE-023
AS/NZS 4187 RELEVANT STANDARDS Robyn Williams NUM CSSD LBHHS & Standards Australia Chair HE-023 Relevant Standards AS 2514 AS 2774 AS 2773 AS 2773.1 Drying cabinets for medical equipment Drying cabinets
More informationGUIDELINE C-17. Non-Incineration Technologies for Treatment of Biomedical Waste (Procedures for Microbiological Testing)
GUIDELINE C-17 Non-Incineration Technologies for Treatment of Biomedical Waste (Procedures for Microbiological Testing) Legislative Authority: Environmental Protection Act, Part V, Sections 19 and 27;
More informationExercise 24-A MICROBIAL CONTROL METHODS (Effects Of Temperature, Ultra Violet Light, Disinfectants And Antiseptics)
Introduction Exercise 24-A MICROBIAL CONTROL METHODS (Effects Of Temperature, Ultra Violet Light, Disinfectants And Antiseptics) Microorganisms, like all other life forms, are greatly influenced by the
More informationEvaluation of Dry Fogging System for Microbial Inactivation
Evaluation of Dry Fogging System for Microbial Inactivation Carol Stansfield Senior Biosafety Officer Safety & Environmental Services Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba
More informationImportant Terminology (pg )
Number of living microbes 10/18/2016 Chapter 9: Control of Microbial Growth 1. Physical Methods 2. Chemical methods Important Terminology (pg. 263-264) sterilization > commercial sterilization > disinfection
More informationIDEXX is an ISO certified facility. Gil Dichter World Wide Technical Support Manager
USEPA ATP Process for Approval of Colilert-18 and Quanti-Tray for the Detection of Fecal Coliforms in Waste Water; What is a Number: Comparison of MPN and CFU Gil Dichter World Wide Technical Support Manager
More informationExercise 19. Fungi: Molds and Yeasts F10 Or The Rotten World Around Us
Exercise 19 119 Fungi: Molds and Yeasts F10 Or The Rotten World Around Us INTRODUCTION: Student Learning Objectives: After completing this exercise students will: a. Define the terms Saprophyte, Mycosis,
More informationSpore concerns and testing in dairy: Reducing variability and establishing standards
Spore concerns and testing in dairy: Reducing variability and establishing standards NICOLE MARTIN (NHW6@CORNELL.EDU) MILK QUALITY IMPROVEMENT PROGRAM DEPARTMENT OF FOOD SCIENCE, CORNELL UNIVERSITY Overview
More informationImpact of Culture Media on Biological Indicator Spore Recovery
Volume 4, Number 1 January 2007 Impact of Culture Media on Biological Indicator Spore Recovery Kellie Matzinger Spore Lab Supervisor The D-value of a specific biological indicator is significantly influenced
More informationSterile Compounding elearning Course Curriculum 28 lessons with 33 hours of CE. Fundamentals of Sterile Compounding (8 lessons/8 hours CE)
Sterile Compounding elearning Course Curriculum 28 lessons with 33 hours of CE CriticalPoint s Sterile Compounding elearning curriculum is written by industry experts and covers both Chapter and
More informationFast decisions instead of delays Quality Control through outstanding design
Fast decisions instead of delays Quality Control through outstanding design We are CustomBiotech from Roche In your operations, behind your decisions, powering your products You are driving a paradigm
More informationFluorescent Sensor Detects Microbes in Real Time
Fluorescent Sensor Detects Microbes in Real Time BioVigilant has developed an optical technology that can determine the quantity and size of particles in liquid or air, and simultaneously determine whether
More informationDisinfection and sterilisation
Disinfection and sterilisation Mongolia 2011 Prof. Dr. Walter Popp Hospital Hygiene, University Clinics Essen, Germany 1 Term Definition Reduction factor of germs Cleaning Remove dirt including 10-100
More informationValidation of cleaning and sterilization of reusable medical devices
Synergy makes sence Validation of cleaning and sterilization of reusable medical devices MG-FSI72-119 Last revision: June 2012 Aurélien BIGNON 5, Chemin du Catupolan - 69120 Vaulx en Velin - France - Tel.
More informationMicrobiological Quality: Understanding Drinking Water Quality and Management
S A S K A T C H E W A N Microbiological Quality: Understanding Drinking Water Quality and Management June 2017 EPB 197 Table of Contents Page Number How to Determine the Microbiological Quality... 1 Monitoring
More informationFAQFrequently. Asked Questions. Education & Training. 3M Sterilization Assurance Techline
Asked Questions 3M Sterilization Assurance Techline by Sandra Velte, BA, CSPDT FAQFrequently Objectives After completion of this self-study activity, the learner will be able to: 1. Develop a policy and
More informationHigh Quality Pipette Tips for Consistent and Reliable Results
High Quality Pipette Tips for Consistent and Reliable Results Excellence in Quality and Convenience Sartorius pipette tips meet the highest quality and purity standards. Sartorius tips are designed and
More informationLab Exercise #4 Microbial Control Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic
Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic I. OBJECTIVES: Investigate the effectiveness various agents of control. Assess the effectiveness of heat in killing vegetative
More informationMycoplasma bovis Staphylococcus aureus Streptococcus agalactiae Prototheca
Mycoplasma bovis Staphylococcus aureus Streptococcus agalactiae Prototheca Cat. No.: M4E USER MANUAL TABLE OF CONTENTS Revision 2016.12.15 TABLE OF CONTENTS 1. PRINCIPLE OF THE TEST... 3 2. KIT COMPONENTS
More informationThe Control of Microbial Growth
The Control of Microbial Growth Sepsis refers to microbial contamination. Asepsis is the absence of significant contamination. Aseptic surgery techniques prevent microbial contamination of wounds. Terminology
More informationUSP Chapter 823 USP 32 (old) vs. USP 35 (new)
USP Chapter 823 USP 32 (old) vs. USP 35 (new) Sally W. Schwarz, MS, BCNP Research Associate Professor of Radiology Washington University School of Medicine St. Louis, MO Why USP Chapter ? FDA has
More informationINTRODUCTION water-soluble Figure 1.
INTRODUCTION Natural waters contain bacteria. The aerobic gram negative bacillus of the genera Psedomonas, Alcalignes, and Flavobacterium are common in natural waters. Many of these bacteria are able to
More informationTHE COST: $836 Billion is the total cost of sick employees
FAR-UV STERILRAY THE FACTS: 1 in 3 people believe they have caught a virus from an ill co-worker (Infection Control Today, 2003) 80% of employees report regularly going to work while sick (University of
More informationSteam-in-Place (SIP), the Problems and a New Solution
BIOLOGICAL INDICATOR SPECIALISTS Volume 3, Number 4 November 2006 Steam-in-Place (SIP), the Problems and a New Solution Rick Gillis, Ph.D. Production Services Manager Validation engineers face many challenges
More informationUSING AN AUTOCLAVE FOR STERILZATION
USING AN AUTOCLAVE FOR STERILZATION These notes are for reference only and are not intended to replace training by your lab supervisor. Ensure you familiarize yourself with your lab protocols and the appropriate
More informationNitrogen Dioxide (NO 2 ) PDA: A Global. Sterilization and. Association. Decontamination Systems. November 4, PDA Europe Parenterals 2014
Nitrogen Dioxide (NO 2 ) PDA: A Global Sterilization and Association Decontamination Systems November 4, 2014 PDA Europe Parenterals 2014 Objectives Review NO 2 processes Sterilization Decontamination
More informationLab 2-Microbial Enumeration
Lab 2-Microbial Enumeration 2/19/08 CE 573 Introduction There are many different techniques that can be utilized when trying to quantify microorganisms found in a given sample. The purpose of this lab
More informationCompounding Pharmacies and the USP <71> Sterility Tests
Compounding Pharmacies and the USP Sterility Tests Scott Sutton, Ph.D. scott.sutton@microbiol.org 49 41 Disclaimer I am making this presentation as an independent agent I am not making this presentation
More informationVirtual Bacterial Identification Lab
Name: Biology Lab: Mr. Zimmerman Date: Period: I. Objectives Virtual Bacterial Identification Lab To familiarize you with the science and techniques used to identify different types of bacteria based on
More informationProject 7: Wound Cultures and Identification
Project 7: Wound Cultures and Identification Readings: https://labtestsonline.org/understanding/analytes/wound-culture/tab/test Identification of Gram-Positive & Gram-Negative Bacteria Guide to laboratory
More informationFinally, You Have a Choice! Online Ordering Available
Simple and Effective System for Colony Counting Finally, You Have a Choice! Online Ordering Available Compact Dry is a ready-touse system reducing the time needed to perform microbial testing on food,
More informationCELL HEALTH. reliable cell viability data. instant time savings. PrestoBlue Cell Viability Reagent
CELL HEALTH reliable cell viability data instant time savings PrestoBlue Cell Viability Reagent PrestoBlue Cell Viability Reagent Why should I use the PrestoBlue Cell Viability Reagent? PrestoBlue reagent
More informationSCHEDULE. Friday: Pet Investigations: Plate counts - how to know how many clones of your pet you have (pg. 9-10)
SCHEDULE Wednesday: Pet Investigations: Phenol Red Broth with Durham tubes (pg. 3-4) Oxidation/Fermentation Agar (pg. 5-6) Anaerobic Growth (pg. 7) Growth in Liquid Culture (pg. 8-9) Friday: Pet Investigations:
More informationTRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE
TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE GENERAL GUIDELINES: Safety Wear a lab coat and have your goggles on! ALWAYS disinfect the tables BEFORE and AFTER lab. Wash your hands with soap both BEFORE
More informationBEST PARTER & BEST SOLUTION RENO Plasma Sterilizer
Web: www.medcon.com.au Email: info@medcon.com.au MED-CON PTY LTD SOLUTIONS FOR BIO-SECURITY Phone: 03 9753 2710 Fax: 03 9753 2722 BEST PARTER & BEST SOLUTION RENO Series - Benefits Rapid Sterilization
More informationHot Topics in Drug Product Process Validation: A Reviewer s Perspective
Hot Topics in Drug Product Process Validation: A Reviewer s Perspective Colleen Thomas, Ph.D. Quality Assessment Lead (Acting) FDA/CDER/OPQ/OPF Division of Microbiology Assessment CASSS CMC Strategy Forum
More informationChoose a category. You will be given the answer. You must give the correct question. Click to begin. Instructions for using this template.
Instructions for using this template. Remember this is Jeopardy, so where I have written Answer this is the prompt the players will see, and where I have Question should be the player s response. To enter
More informationPreLab Activity: Read through the entire lab! Come prepared to explain to your group what this lab is about
BIO101Lab weeks 4 and 5 PreLab Activity: Read through the entire lab! Come prepared to explain to your group what this lab is about The Evolution of the bacterium, Pseudomonas fluorescens Introduction
More informationGuidance. Media Fills for Validation of Aseptic Preparations for Positron Emission Tomography (PET) Drugs DRAFT GUIDANCE
Guidance Media Fills for Validation of Aseptic Preparations for Positron Emission Tomography (PET) Drugs DRAFT GUIDANCE This guidance document is being distributed for comment purposes only. Comments and
More informationMicrofil Filtration System
Data Sheet Microfil Filtration System An easy to use, fast and accurate system for the routine microbiological analysis of your beverages and drinking water The Microfil filtration system is a simple and
More informationThe Golden Age of Microbiology Discovering the Cause of Disease. Packet #3
The Golden Age of Microbiology Discovering the Cause of Disease Packet #3 Scientists searched for answers to four questions: Is spontaneous generation of microbial life possible? What causes fermentation?
More informationEnviroDEFENSE PurPond Aquaculture Application: Tiger Prawn Aquaculture Ponds
FIELD STUDY REPORT EnviroDEFENSE PurPond Aquaculture Application: Tiger Prawn Aquaculture Ponds Product Overview EnviroDEFENSE PurPond Aquaculture is a dry bacterial blend designed to remove organic matter
More informationAutomated methods in the Microbiology Lab-Issues and Troubleshooting! Presented By: Dr B. Oboudi
In the name of GOD Automated methods in the Microbiology Lab-Issues and Troubleshooting! Presented By: Dr B. Oboudi Overview Background/history Plate streakers and Gram stainers Blood cultures Automated
More informationMicrobial Growth and The Control of Microbial Growth (Chapter 6 & 7)
Microbial Growth and The Control of Microbial Growth (Chapter 6 & 7) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Eastern Campus Primary Source for figures and content:
More informationCHECK YOUR for your CE certification of completion.
2017 course #3 self-study course contact us: phone 614-292-6737 toll free 1-888-476-7678 fax 614-292-8752 e-mail smsosu@osu.edu web dentistry.osu.edu/sms The Ohio State University College of Dentistry
More information