CSA II Biotin-free Tyramide Signal Amplification System. Code K1497 For use with mouse primary antibodies. Limited use license
|
|
- Samuel Campbell
- 5 years ago
- Views:
Transcription
1 CSA II Biotin-free Tyramide Signal Amplification System Code K1497 For use with mouse primary antibodies Limited use license This system embodies technology developed by and licensed from NEN Life Science Products, Inc. U.S. Patent No. 5,196,306. This product is distributed and sold to the End-User pursuant to a license from NEN LIFE SCIENCE PRODUCTS, INC. for use by the End-User in manual or automated processing on DakoCytomation instrumentation only of glass microscope slides or other support material (other than microarrays and bio-chips) containing cell or tissue specimens for examining those specimens under a microscope (including automated image capture and analysis systems) for the purpose of detecting target nucleic acids or proteins. Purchase does not include or carry any right to resell or transfer this product either as a stand alone product or as a component of another product. Any use of this product other than the licensed use without the express written authorization of NEN LIFE SCIENCE PRODUCTS, INC. is strictly prohibited. Intended use For In Vitro diagnostic use. These instructions apply to the CSA II (code K1497). CSA II is a biotin-free tyramide signal amplification system for immunohistochemistry. This system is intended for use with primary antibodies from mouse supplied by the user for the qualitative identification of antigens by light microscopy in normal and pathological formalin-fixed, paraffin-embedded tissues. This system may be used with manual procedures and/or with DakoCytomation instrumentation. Refer to the General Instructions for Immunohistochemical Staining of IHC procedures for: (1) Principle of Procedure, (2) Materials Required, Not Supplied, (3) Storage, (4) Specimen Preparation, (5) Staining Procedure, (6) Quality Control, (7) Troubleshooting, (8) Interpretation of Staining, (9) General Limitations. Summary and explanation Principles of procedure The CSA II System is a highly sensitive immunohistochemical (IHC) staining procedure incorporating a signal amplification method based on the peroxidase-catalyzed deposition of a fluorescein-labelled phenolic compound, followed by a secondary reaction with a peroxidase-conjugated anti-fluorescein. 1-5 In the procedure, a mouse primary antibody is first detected with a peroxidase-conjugated secondary antibody. The next step utilizes the bound peroxidase to catalyze oxidation of a fluorescein-conjugated phenol (fluorescyl-tyramide) which then precipitates onto the specimen. The procedure is continued with detection of the bound fluorescein by a peroxidase-conjugated anti-fluorescein. Staining is completed using diaminobenzidine/hydrogen peroxide as chromogen/substrate, and can be observed with a light microscope. In comparison to standard immunohistochemical methods, such as labelled streptavidin biotin (LSAB) or avidin-biotin complexes (ABC), tyramide amplification methods have been reported to be many fold more sensitive. 3,4 The CSA II System is a simplified version of the extremely sensitive Catalyzed Signal Amplification System (code K1500) that utilizes biotinyl-tyramide. The highly sensitive CSA II System allows for the detection of very small quantities of target protein, as well as for the use of low affinity antibodies. This reagent system utilizes fluorescyl-tyramide, rather than biotinyl-tyramide, and does not contain avidin/biotin reagents, thus eliminating potential background staining due to reactivity with endogenous biotin. 6,7 The specimens are first incubated with Peroxidase Block for five minutes to quench endogenous peroxidase activity. The specimens are then incubated for five minutes with a protein block to suppress nonspecific binding of subsequent reagents, followed by a 15-minute incubation with an appropriately characterized and diluted mouse primary antibody or negative control reagent (user provided). This is followed by sequential 15-minute incubations with anti-mouse immunoglobulins-hrp, fluorescyl-tyramide hydrogen peroxide (amplification reagent) and anti-fluorescein-hrp. Staining is completed by a five-minute incubation with 3,3' diaminobenzidine tetrahydrochloride (DAB)/hydrogen peroxide, which results in a brown precipitate at the antigen site. ( ) EFG_ p. 1/10
2 Reagent provided Code K1497 The following materials are included in this kit: Quantity 1x15 ml Description Peroxidase Block 3% hydrogen peroxide in water. 1x15 ml Protein Block Serum-free protein in buffer with mol/l sodium azide. 1x15 ml Anti-Mouse Immunoglobulins-HRP Anti-mouse immunoglobulins conjugated to horseradish peroxidase in buffer containing stabilizing protein and an antimicrobial agent. 1x15 ml Amplification Reagent Fluorescyl-tyramide and hydrogen peroxide in buffer containing stabilizing protein and an antimicrobial agent. 1x15 ml Anti-Fluorescein-HRP Anti-fluorescein antibody conjugated to horseradish peroxidase in buffer containing stabilizing protein and an antimicrobial agent. 1x18 ml Buffered Substrate Buffer containing hydrogen peroxide and a preservative. 1x1 ml Liquid DAB Chromogen 3,3'-diaminobenzidine chromogen solution. Materials required, but not supplied Antibody diluent, such as Antibody Diluent with Background Reducing Components (code S3022) Control specimens, positive and negative Counterstain, such as Lillie s Modified Mayer s Hematoxylin (code S3301 for use on the Dako Autostainer or code S3302 for Manual Staining) Light microscope Mounting media such as Glycergel Mounting Medium (code C0563), Faramount, Aqueous Mounting Medium, Ready-to-use (code S3025), or a nonaqueous permanent mounting medium, such as Permanent Mounting Media (code S3026) Mouse primary antibodies and negative control reagents Wash buffer, such as TBST (Tris Buffered Saline With Tween), 10x Concentrate (code S3306) Automated Staining Dako Autostainer (code S3400) or Autostainer Plus (code S3800). Refer to the Dako Autostainer User Guide for necessary components Optional materials required, but not supplied Refer to primary antibody specification sheet Target retrieval solution, modified citrate buffer (ph 6.1), such as Target Retrieval Solution, Ready-to-use (code S1700) or 10x Concentrate (code S1699) Water bath (capable of maintaining C), steamer, pressure cooker or autoclave for performing target retrieval ( ) EFG_ p. 2/10
3 Precautions Product Specific 1. For professional users. 2. This product contains sodium azide (NaN 3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, build-ups of NaN 3 may react with lead and copper plumbing to form highly explosive metal azides. Upon disposal, flush with large volumes of water to prevent azide build-up in plumbing. 8,24 3. Minimize microbial contamination of reagents or increase in nonspecific staining may occur. 4. As with any product derived from biological sources, proper handling procedures should be used. 5. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 6. Unused solution should be disposed of according to local, State and Federal regulations. 7. Safety Data Sheet available for professional users on request. General 1. Do not use reagents beyond expiration date. 2. Do not substitute reagents from kits of other manufacturers. 3. Do not store system components or perform staining in strong light, such as direct sunlight. Note: Slides must be protected from light during the Amplification Reagent incubation step. Risk and Safety Statements Dab Chromogen R 40 Limited evidence of a carcinogenic effect. R43 May cause sensitization by skin contact. R68 Possible risk of irreversible effects. S35 This material and its container must be disposed of in a safe way. S 36/37 Wear suitable protective clothing and gloves. Storage Reagents of CSA II System are to be stored at 2 8 C. Do not freeze. Do not use after expiration printed on reagent vials and kit label. There are no obvious signs to indicate instability of these products. Therefore, positive and negative controls should be tested simultaneously with patient specimens. If unexpected staining is observed which cannot be explained by variation in laboratory procedures and a problem with the kit is suspected, contact DakoCytomation Technical Support. Reagent preparation Wash Buffer Investigation has shown that 0.05 mol/l Tris-HCl, ph 7.6, containing 0.3 mol/l NaCl and 0.1% Tween 20, without sodium azide, significantly aids in minimizing background staining with this system. A recommended wash buffer is TBST 10x (code S3306). Wash buffers containing sodium azide inactivate horseradish peroxidase and result in negative staining. Store unused buffer at 2 8 C. Discard buffer if cloudy in appearance. Automated Staining Automation Buffer, 10x (code S3006) can be used for the general wash buffer rinses on the Dako Autostainer for the CSA II automated protocol, but a 5 10 minute auxiliary wash with TBST (code S3306) should be used after the hydrogen peroxide, the primary antibody and the anti-mouse-hrp reagents. Distilled or deionized water may be used for rinsing after the substrate-chromogen and counterstain steps. Manual Staining Tissue sections should be washed in up to three fresh baths of TBST (code S3306) for 3 5 minutes between each step of the CSA II protocol. DAB Substrate-Chromogen Preparation One ml of the substrate-chromogen is sufficient for up to ten tissue sections. STEP 1 STEP 2 Depending on the number of slides to be stained, transfer enough 1 ml aliquots of buffer from the Buffered Substrate bottle into a test tube. For each 1 ml of Buffered Substrate, add 1 drop (20 µl) from the Liquid DAB Chromogen bottle. Mix immediately. The prepared substrate-chromogen is stable approximately five days when stored at 2 8 C. This reagent should be mixed thoroughly prior to use. Any precipitate developing in the reagent does not affect staining quality. Dilution of Concentrated Primary Antibody and Negative Control Dilute primary antibody using a diluent containing stabilizing protein. Use of Antibody Diluent with Background Reducing Components (code S3022) is highly recommended for minimizing background staining. An appropriate negative control reagent consists of mouse immunoglobulin of the same isotype as the primary antibody diluted to an immunoglobulin concentration equivalent to that of the diluted primary antibody. ( ) EFG_ p. 3/10
4 Specimen preparation Paraffin-embedded specimen preparation Prior to IHC staining, tissues must be fixed and processed. Fixation prevents autolysis and putrefaction of excised tissues, preserves antigenicity, enhances the refractive index of tissue constituents and increases the resistance of cellular elements to tissue processing. Tissue processing includes dehydration, clearing of dehydrating agents, infiltration of embedding media, embedding and sectioning of tissues. The most common fixatives for IHC tissue preparations are discussed in the General Instructions for Immunohistochemical Staining. These are guidelines only. Optimal procedures must be determined and verified by the user. Survival of tissue antigens for immunological staining may depend on the type and concentration of fixative, on fixation time, and on the size of the tissue specimen to be fixed It is important to maintain optimal, standardized fixation conditions whenever possible in order to obtain reproducible staining. Where possible, use of thinner specimens coupled with shorter fixation times is recommended. Prolonged exposure to fixatives may result in the masking of antigens and contribute to reduced staining. After fixation, tissues are dehydrated using graded alcohols, cleared with xylene or xylene substitute, and infiltrated with paraffin wax. The tissue is subsequently embedded with paraffin wax in molds or cassettes, which facilitate tissue sectioning. To minimize denaturing of antigens, do not expose tissues to temperatures in excess of 60 C during processing. Tissue blocks may be stored or sectioned on completion of embedding. Properly fixed and paraffin-embedded tissues will keep indefinitely if stored in a cool place (15 22 C). Mounting of Tissue Sections Sectioned tissues cut from paraffin-embedded blocks are collected on clean glass slides. Tap slides to remove as much water as possible. Water droplets remaining on the tissue during the drying process may adversely affect tissue staining. Dry in an oven for one to two hours at 60 C or less or allow to dry at room temperature for hours. For increased adhesion of tissue sections during IHC staining procedures, use of poly-l-lysine coated slides, charged slides or Silanized Slides (code S3003) is suggested. Use of Silanized or charged slides is strongly recommended for staining procedures requiring target retrieval. Automated and manual staining procedure Procedural Notes The user should read these instructions carefully and become familiar with the kit contents prior to use. All reagents should be equilibrated to room temperature (20 25 C) prior to immunostaining; likewise, all incubations are optimized for performance at room temperature. The reagents and instructions supplied in this kit have been designed for optimal performance. Further dilution of the ready-to-use reagents may give erroneous results. Note: For Automated Staining, the tinted Dako Autostainer cover should be closed during the Amplification Reagent step for optimal staining performance. For Manual Staining, protect slides from light during the incubation of the Amplification Reagent. Do not allow tissue sections to dry during the staining procedure. Dried tissue sections may display nonspecific staining. Cover slides exposed to drafts. If prolonged incubations are used, place slides in a humid environment. For reduction of nonspecific background staining it is recommended that tissue sections be rinsed with TBST according to the wash buffer recommendations in Reagent Preparation section. Deparaffinization of Tissue Sections Prior to staining, tissue slides must be deparaffinized to remove embedding medium and then rehydrated. Avoid incomplete removal of paraffin. Residual embedding medium will result in increased nonspecific staining. Proteolytic Digestion and Target Retrieval Formaldehyde is known to induce conformational changes in the antigen molecules by forming intermolecular cross-linkages. Excessive formalin fixation can mask antigenic sites and diminish specific staining. In standard IHC procedures, these sites may be revealed with proteolytic digestion of tissue prior to immunostaining. Note: Use of proteolytic digestion may result in high background staining with the CSA II System and is therefore not recommended. Target retrieval using heat is the preferred alternative to proteolytic digestion, resulting in a slight increase in background staining concurrent with a significant increase in specific staining with many primary antibodies. To determine if target retrieval treatment of tissues is warranted, see the specification sheet provided with the primary antibody; for some primary antibodies this procedure is required. The recommended target retrieval procedure involves immersion of tissue sections mounted on slides in a modified citrate buffer (ph 6.1) such as Target Retrieval Solution, Ready-to-use (code S1700), or 10x Concentrate (code S1699); refer to package insert for complete instructions and heating prior to IHC staining. Rinse thoroughly with wash buffer solution and continue with the staining procedure. Note: Increased background staining may result from the use of a high ph (ph 10) target retrieval solution with CSA II System and is therefore not recommended. ( ) EFG_ p. 4/10
5 Automated Staining Procedure STEP 1 Select Protocol Template Auto Program STEP 2 Place reagents and buffer in the Dako Autostainer reagent rack STEP 3 Load the slides on to the Dako Autostainer STEP 4 Start the run STEP 5 Remove slides from the Dako Autostainer STEP 6 Proceed with mounting Note: Counterstaining is included in the recommended programming grid. Counterstaining can be run manually on the lab bench as an alternative option. Manual Staining Procedure STEP 1 PEROXIDASE BLOCK Tap off excess liquid. Using a lintless tissue, carefully wipe around the specimen to remove any remaining liquid and to keep reagent within the prescribed area. Apply enough Hydrogen Peroxide to cover specimen. Incubate 5 (±1) minutes. Rinse gently with distilled water or wash buffer solution from a wash bottle (do not focus flow directly on tissue) and place in a fresh wash buffer bath. STEP 2 STEP 3 STEP 4 STEP 5 STEP 6 STEP 7 PROTEIN BLOCK Tap off excess liquid and wipe slide as before. Apply enough Protein Block to cover specimen. Incubate 5 (±1) minutes. Do not rinse off protein block. PRIMARY ANTIBODY OR NEGATIVE CONTROL REAGENT Tap off excess Protein Block and wipe slide as before. Apply enough user-prepared primary antibody or negative control reagent to cover specimen. Incubate 15 (±1) minutes unless otherwise specified in primary antibody specification sheet. Rinse gently with wash buffer solution from a wash bottle and place in up to three fresh TBST buffer baths for 3 5 minutes each. ANTI-MOUSE IMMUNOGLOBULINS-HRP Wipe slide as before. Apply enough Anti-Mouse Immunoglobulins-HRP to cover specimen. Incubate 15 (±1) minutes. Rinse slide as in Step 3. Place in up to three fresh wash buffer baths for 3 5 minutes each. AMPLIFICATION REAGENT Note: Slides must be protected from light during this incubation step for optimal staining. Wipe slide as before. Apply enough Amplification Reagent to cover specimen. Incubate 15 (±1) minutes protected from light. Rinse slide as before. Place in up to three fresh wash buffer baths for 3 5 minutes each. ANTI-FLUORESCEIN-HRP Wipe slide as before. Apply enough Anti-Fluorescein-HRP to cover specimen. Incubate 15 (±1) minutes. Rinse slide as before. Place in up to three fresh wash buffer baths for 3 5 minutes each. LIQUID DAB SUBSTRATE-CHROMOGEN Wipe slide as before. Apply enough prepared Liquid DAB Substrate-Chromogen to cover specimen. Incubate 5 (±1) minutes. Rinse with distilled or deionized water. ( ) EFG_ p. 5/10
6 STEP 8 PROCEED WITH COUNTERSTAIN AND MOUNTING Dako Autostainer Programming Grid for CSA II The programming grid above is a DakoCytomation Protocol Template containing protocol elements to run CSA II. This example includes the recommended reagents and incubation times for an optimal CSA II run for the Autostainer (code S3004) and Autostainer Plus (code S3800). Set up a protocol template and Auto Program with these recommended steps and incubation times. The primary antibody and pretreatment has been inserted as an example. Application of Target Retrieval is antibody specific and may not be required. Quality control Differences in tissue processing and technical procedures in the user s laboratory may produce significant variability in results, necessitating regular performance of in-house controls. See references 16 through 18 for additional quality control information. Positive Control Tissue Known positive control tissues should be utilized for monitoring the correct performance of processed tissues and test reagents. If the positive control tissues fail to demonstrate positive staining, results with the test specimens should be considered invalid. Negative Control Reagent Use a negative control reagent in place of the primary antibody with a section of each test specimen to evaluate nonspecific staining and to allow better interpretation of specific staining at the antigen site. If panels of antibodies are used on serial tissue sections, the negatively staining areas of one slide may serve as a negative/nonspecific binding background control for other antibodies. Refer to the General Instructions for Immunohistochemical Staining for further information on positive and negative controls. Staining interpretation The positive control specimens should be examined first to ascertain that all reagents are functioning properly. The presence of a brown-colored end product at the site of the target antigen is indicative of positive reactivity. If the positive control specimens fail to demonstrate positive staining, results with the test specimens should be considered invalid. The negative control specimens should be examined to verify the specific labelling of the target antigen by the primary antibody. The absence of specific staining in the negative control specimens confirms the specificity of the primary antibody. Nonspecific staining, if present, will be of dot-like or diffuse appearance. The high sensitivity of this system is due to its great level of signal amplification; any nonspecific background staining present will also be amplified. For this reason, use of negative control reagents on a section of each control and test specimen is recommended as an aid in interpretation of staining (see Limitations for more discussion on nonspecific staining). Sporadic light staining of connective tissue may be observed in sections from excessively formalin-fixed tissues. Use intact cells for the interpretation of staining results. Necrotic or degenerated cells often stain nonspecifically. False-positive results may be seen due to non-immunologic binding of reagents to tissue sections. ( ) EFG_ p. 6/10
7 Limitations For optimal staining intensity, protect slides from light during the Amplification Reagent incubation (Step 5). Use of proteolytic digestion may result in high background staining with the CSA II System and is therefore not recommended. For reduction of nonspecific background staining it is recommended that tissue sections be rinsed with wash buffer (see Reagent Preparation) and incubated at room temperature in up to three fresh baths of wash buffer for three to five minutes each between each reagent step in the CSA II System protocol. Endogenous immunoglobulin, present in human tissue specimens, may exhibit various levels of cross-reactivity with secondary anti-immunoglobulin antibodies. The anti-mouse immunoglobulin-hrp secondary antibody used in this system has been absorbed with normal human serum to reduce cross-reactivity. However, due to the high sensitivity of this system, cross-reactivity to endogenous immunoglobulin may still be detected in some specimens. Background staining due to endogenous immunoglobulin is best recognized as staining in the negative control around blood and lymph vessels, in connective tissue, and in extravascular tissue spaces. Certain types of epithelium, particularly those associated with the digestive tract, may also display endogenous immunoglobulin. Staining due to endogenous immunoglobulin may also be increased by using target retrieval methods. For this reason, use of appropriate negative control reagents and tissues are required for interpreting any positive staining where presence of endogenous immunoglobulin is suspected. Endogenous peroxidase or pseudoperoxidase activity can be found in hemoproteins such as hemoglobin, myoglobin, cytochrome, and catalase, as well as in certain leucocytes. 19,20 This activity can be eliminated by incubating specimens with Peroxidase Block for 5 minutes (Step 1 of the CSA II System protocol) prior to the application of the primary antibody. Tissues containing hepatitis B surface antigen (HBsAg) may exhibit nonspecific staining with horseradish peroxidase. 21 Automated troubleshooting Problem Probable Cause Suggested Action 1. No staining of slides. 1a. Programming error. 1a. Review application of reagents. Reagents not used in proper order. 1b. Reagent vials were not loaded in the correct locations in the reagent racks. 1b. Check the Reagent Map to verify the proper location of reagent vials. 2. Weak staining of slides. 3. Excessive background staining of slides. 1c. Insufficient reagent in reagent vial. 1c. Ensure that enough reagent is loaded into the reagent vials prior to beginning the run. Refer to Reagent Map for volumes required. 1d. Sodium azide in buffer bath. 1d. Use fresh, azide-free buffer. 2a. Slides not incubated long 2a. Review recommended incubation times. enough with antibodies or substrate. 2b. Antigen under investigation requires target retrieval. 2c. Specimens exposed to light during Amplification Reagent incubation. 2d. Antibody concentration too low. 3a. Specimens contain high levels of endogenous immunoglobulin. 3b. Specimens contain high endogenous peroxidase activity. 3c. Paraffin incompletely removed. 2b. Perform target retrieval. 2c. Protect specimens from light during Amplification Reagent incubation. 2d. Re-titer antibody. 3a. Compare test tissue with the negative control tissue for differences. 3b. Use longer incubation time of hydrogen peroxide (Step 1). 3c. Use fresh xylene or toluene baths. If several slides are stained simultaneously, the second xylene bath should contain fresh xylene. 3d. Slides not thoroughly rinsed. 3d. Ensure that the Dako Autostainer is properly primed prior to running. Check to make sure that adequate buffer is provided for entire run. Include auxiliary washes with TBST (code S3306) as specified. ( ) EFG_ p. 7/10
8 4. Tissue detached from slides. 3e. Sections dried during staining. 3f. Slides dried while loading the Dako Autostainer. 3g. Nonspecific binding of reagents to tissue section. 3h. Nonspecific binding of primary antibody to specimen. 3i. High ph Target Retrieval Solution used for heat retrieval prior to staining. 3j. Proteolytic digestion used 3e. Verify that the appropriate volume of reagent is being applied to slides. Make sure the Dako Autostainer is run with the hood in the closed position and that it is not exposed to excessive heat or drafts. 3f. Ensure slides remain wet with buffer while loading and prior to initiating the run. 3g. Use longer incubation time of Protein Block (Step 2). 3h. Dilute primary antibody in diluent containing carrier protein. Antibody Diluent with Background Reducing Components (code S3022) is recommended. 3i. Use a modified citrate buffer (ph 6.1) for target retrieval. 3j. Avoid use of proteolytic digestion with this prior to staining. staining system. 4a. Use of incorrect slides. 4a. Use charged (SuperFrost Plus) or Silanized slides such as DakoCytomation s Silanized Slides (code S3003). Manual troubleshooting Problem Probable Cause Suggested Action 1. No staining of slides. 1a. Reagents not used in proper 1a. Review application of reagents. order. 1b. Sodium azide in buffer bath. 1b. Use fresh, azide-free buffer. 2. Weak staining of slides. 2a. Sections retain too much solution after wash bath. 2a. Gently tap off excess solution before wiping around slides. 2b. Slides not incubated long 2b. Review recommended incubation times. enough with antibodies or substrate. 2c. Antigen under investigation 2c. Perform target retrieval. requires target retrieval. 2d. Specimens exposed to light during Amplification Reagent 2d. Protect specimens from light during Amplification Reagent incubation. incubation. 2e. Antibody concentration too 2e. Re-titer antibody. 3. Excessive background staining of slides. low. 3a. Specimens contain high levels of endogenous immunoglobulin. 3b. Specimens contain high endogenous peroxidase activity. 3c. Paraffin incompletely removed. 3a. Compare test tissue with the negative control tissue for differences. 3b. Use longer incubation time of hydrogen peroxide (Step 1). 3c. Use fresh xylene or toluene baths. If several slides are stained simultaneously, the second xylene bath should contain fresh xylene. 3d. Slides not thoroughly rinsed. 3d. Use fresh solutions in buffer baths and wash bottles. TBST is recommended. Incubation sections in up to three fresh TBST baths for 3 5 minutes each between protocol steps. 3e. Faster than normal substratechromogen reaction due to excessively high room temperature. 3f. Sections dried during staining procedure. 3g. Nonspecific binding of reagents to tissue section. 3h. Nonspecific binding of primary antibody to specimen. 3e. Use shorter incubation time with substrate-chromogen solution. 3f. Use humidity chamber. Wipe only 3 to 4 slides at a time before applying reagent. 3g. Use longer incubation time of Protein Block (Step 2). 3h. Dilute primary antibody in diluent containing stabilizing protein. Antibody Diluent with Background Reducing Components (code S3022) is recommended. ( ) EFG_ p. 8/10
9 4. Tissue detached from slides. 5. Run-to-run variability of staining intensity. 3i. High ph Target Retrieval Solution used for heat Retrieval prior to staining. 3j. Proteolytic digestion used 3i. Use a modified citrate buffer (ph 6.1) for target retrieval. 3j. Avoid use of proteolytic digestion with this prior to staining. staining system. 4a. Use of incorrect slides. 4a. Use charged (SuperFrost Plus) or Silanized slides such as DakoCytomation s Silanized Slides (code S3003). 5a. Variations in room temperature. 5b. Slight variations in length of reagent incubations. 5c. Variations in number and incubation times of wash buffer baths. 5a. Regulate room temperature to narrow range. 5b. Time incubations consistently. 5c. Follow identical protocol run-to-run. References 1. Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J Immunol Methods 1989; 125: Bobrow MN, Shaughnessy KJ, Litt GJ. Catalyzed reporter deposition, a novel method of signal amplification. II. Application to membrane immunoassays. J Immunol Methods 1991; 137: Van Gijlswijk RPM, Zijlmans HJMAA, Wiegant J, Bobrow MN, Erickson TJ, Adler KE, Tanke HJ, Raap AK. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem 1997; 45(3): Von Wasielewski R, Mengel M, Gignac S, Wilkens L, Werner M, Georgii A. Tyramine amplification technique in routine immunohistochemistry. J Histochem Cytochem 1997; 45(11): Bobrow MN, Litt GJ, Shaughnessy KJ, Mayer PC, Conlon J. The use of catalyzed reporter deposition as a means of signal amplification in a variety of formats. J Immunol Methods 1992; 150: Wood GS and Warnke R. Suppression of endogenous avidin-biotin activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem 1981; 29(10): Banerjee D and Pettit S. Endogenous avidin-binding activity in human lymphoid tissue. J Clin Pathol 1984; 37: Department of Health, Education and Welfare, National Institute for Occupational Safety and Health, Rockville, MD. Procedures for the decontamination of plumbing systems containing copper and/or lead azides. DHHS (NIOSH) Publ. No , Current 13. August 16, National Committee for Clinical Laboratory Standards. Protection of laboratory workers from instrument biohazards and infectious disease transmitted by blood, body fluids and tissue; approved guideline. Villanova, PA. 1997; Order Code M29-A 10. Kiernan JA. Histological and histochemical methods: Theory and practice. New York: Pergamon Press 1981; Boenisch T. In: Naish SJ, ed. Handbook immunochemical staining methods. Carpinteria: DakoCytomation Nadji M and Morales AR. Immunoperoxidase: I. The technique and its pitfalls. Lab Med 1983; 14: Banks PM. Diagnostic applications of an immunoperoxidase method in hematopathology. J Histochem Cytochem 1979; 27: Culling CFA, Reid PE, Sinnott NM. The effect of various fixatives and trypsin digestion upon the staining of routine paraffin-embedded sections by the peroxidase-antiperoxidase and immunofluorescent technique. J Histotech 1980; 3: Carson FL (ed). Histotechnology: A self-instructional text. Chicago: ASCP Press 1990: Elias JM, Gown AM, Nakamura RM, Wilbur DC, Herman GE, Jaffe ES, Battifora H, Brigati DJ. Special report: Quality control in immunohistochemistry. Amer J Clin Pathol 1989; 92: National Committee for Clinical Laboratory Standard. Internal quality control testing: principles and definitions; approved guideline. Villanova, PA 1991; Order code C24-A:4 18. Herman GE and Elfont EA. The taming of immunohistochemistry: The new era of quality control. Biotech & Histochem 1991; 66: Escribano LM, Gabriel LC, Villa E, Navarro JL. Endogenous peroxidase activity in human cutaneous and adenoidal mast cells. J Histochem Cytochem 1987; 35: Elias JM. Immunohistopathology: A practical approach to diagnosis. Chicago: Amer Soc Clin Pathol Press 1990; Omata M, Liew C-T, Ashcavai M, Peters RL. Nonimmunologic binding of horseradish peroxidase to hepatitis B surface antigen: A possible source of error in immunohistochemistry. Amer J Pathol 1980; 73: Tubbs RR, Gephardt GN, Petras RE. Atlas of immunohistology. Chicago: Amer Soc Clin Pathol Press Nadji M and Morales AR. Immunoperoxidase techniques, a practical approach to tumor diagnosis. Chicago: Amer Soc Clin Pathol Press Center for Disease Control Manual Guide Safety Management, No. CDC-22, Atlanta, GA. Decontamination of laboratory sink drains to remove azide salts. April 30, 1976 ( ) EFG_ p. 9/10
10 Σ ( ) EFG_ p. 10/10
For research use only. Not for use in diagnostic procedures.
Dako LSAB 2 System-HRP for use on Rat Specimens Code K0609 Intended use For research use only. Not for use in diagnostic procedures. These instructions apply to the LSAB 2 System-HRP for use on RAT SPECIMENS,
More informationLAMININ. For Immunohistochemical Demonstration of Laminin in Paraffin-embedded and Frozen Human Tissue Sections Stock No. IMMH-7
LAMININ For Immunohistochemical Demonstration of Laminin in Paraffin-embedded and Frozen Human Tissue Sections Stock No. IMMH-7 TABLE OF CONTENTS BACKGROUND AND PRINCIPLE... 4 REAGENTS AND EQUIPMENT PROVIDED...
More informationCell & Tissue Staining Kit
Cell & Tissue Staining Kit For the detection of goat, mouse, rabbit, rat, or sheep primary IgG Antibodies Size: 50 Tests HRP-DAB System Goat Kit (Catalog Number CTS008) Mouse Kit (Catalog Number CTS002)
More informationI n s t ru c t i o n s D AKO LSAB 2 System, P e r o x i d a s e
I n s t ru c t i o n s D AKO LSAB 2 System, P e r o x i d a s e Universal Code No. K0672 K0673 K0675 Size 15 ml 15 ml 110 ml For Laboratory Use. 1. INTENDED USE FOR LABORATORY USE These instructions apply
More informationManufactured by. Zyagen Barnes Canyon Road San Diego, CA 92121, USA
Alkaline Phosphatase Immunohistochemistry Detection kits For detection of mouse, rabbit, goat, rat, sheep, chicken, guinea pig, and human primary antibodies Size: 500 Tests Catalog #: AK-011, Mouse Kit
More informationBrdU IHC Kit. For the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells
K-ASSAY BrdU IHC Kit For the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells Cat. No. KT-077 For Research Use Only. Not for Use in
More informationab TripleStain IHC Kit: M&M&R on human tissue (DAB, Red/AP & DAB/Ni)
ab183287 TripleStain IHC Kit: M&M&R on human tissue (DAB, Red/AP & DAB/Ni) Instructions for Use For the detection of Rabbit and Mouse Primary antibodies on Human tissue or cell samples. This product is
More informationab EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit
Version 7 Last updated 17 January 2018 ab80436 - EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit For the detection of a specific antibody bound to an antigen in tissue sections. This product
More informationab Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC Kit
ab64264 - Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC Kit Instructions for Use For the detection of a specific antibody bound to an antigen in tissue sections. This product is for research use
More informationab Human on human IHC kit (AP/Permanent
Version 1 Last updated 13 September 2016 ab214753 Human on human IHC kit (AP/Permanent Red) For staining human primary antibodies on human tissues without background staining This product is for research
More informationab BrdU Immunohistochemistry Kit
ab125306 - BrdU Immunohistochemistry Kit Instructions for Use For the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells. This product
More informationAnti-Piscirickettsia salmonis monoclonal antibody. Product no: P05
Anti-Piscirickettsia salmonis monoclonal antibody Product no: P05 Product Description The monoclonal antibody (Mab) against Piscirickettsia salmonis is specific for this bacterium. The specificity of the
More informationab Mouse and Rabbit AP/Fast-Red (ABC) Detection IHC Kit
ab128967 - Mouse and Rabbit AP/Fast-Red (ABC) Detection IHC Kit Instructions for Use For the detection of a specific antibody bound to an antigen in tissue sections. This product is for research use only
More informationEnzMet HRP Detection Kit for IHC / ISH
EnzMet HRP Detection Kit for IHC / ISH 95 Horseblock Road, Unit 1, Yaphank NY 11980-9710 Tel: (877) 447-6266 (Toll-Free in US) or (631) 205-9490 Fax: (631) 205-9493 Tech Support: (631) 205-9492 tech@nanoprobes.com
More information1. Paraffin section slides can be stored at room temperature for a long time.
Immunohistochemistry (IHC) Protocols Immunohistochemistry (IHC) Protocol of Paraffin Section 1. Fix dissected tissues with 10% formalin for no less than 48 hours at room temperature. Inadequately fixation
More informationHBeAg and HBeAg Ab ELISA Kit
HBeAg and HBeAg Ab ELISA Kit Catalog Number KA0290 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General
More informationHypoxyprobe Plus Kit
Updated 2017 1 PRODUCT INSERT Hypoxyprobe, Inc 121 Middlesex Turnpike Burlington, MA 01803 USA www.hypoxyprobe.com Hypoxyprobe Plus Kit (HPI Part # HP2-XXX) Kit contents: Solid pimonidazole HCl (Hypoxyprobe
More informationAcetyl-p53 (K381) Cell-Based Colorimetric ELISA Kit
Acetyl-p53 (K381) Cell-Based Colorimetric ELISA Kit Catalog No. KA8015C Detection and Quantification of Acetyl-p53 (K381) Protein Concentration in Cell. Research Purposes Only. Not Intended for Diagnostic
More informationInstructions DAKO In situ Hybridization Detection System for Fluorescein-Labelled Probes
Instructions DAKO In situ Hybridization Detection System for Fluorescein-Labelled Probes Code No. K0607 Size 15mL FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. 1. INTENDED USE FOR RESEARCH
More informationHuman Myostatin, ELISA Kit (MSTN)
Human Myostatin, ELISA Kit (MSTN) 96 Tests Catalog Number: MBS733837 Store all reagents at 2-8 C Valid Period: six months For samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood
More informationMethodology for Immunohistochemistry. Learning Objectives:
Proteomics Methodology for Immunohistochemistry Methodology for Immunohistochemistry A staining process for identifying the proteins location in cells, tissues by using antigen-antibody property. Immuno
More informationBovine IgG ELISA Kit
Bovine IgG ELISA Kit Cat. No. E11-118 Components Supplied Bovine IgG Pre-Coated 96-well Strip Plate, 1 each Bovine IgG Standard, 500 ng/vial, 2 each Bovine IgG Detection Antibody, 12 ml 20X Dilution Buffer
More informationProduct Datasheet and Instructions for Use
Product Code: MP-357-CMK01 (conc 0.1ml) MP-357-CMK05 (conc 0.5ml) MP-357-CMK1 (conc 1ml) MP-357-PM6 (RTU 6ml) Product Description: Factor XIIIa Concentrated and Prediluted Monoclonal Antibody Control Number:
More informationCyfra 21-1 IRMA. Product information Information about other products is available at: Userś Manual DE52100
Product information Information about other products is available at: www.demeditec.com Userś Manual Cyfra 21-1 IRMA The CYFRA 21.1 IRMA system provides a direct in vitro quantitative determination of
More informationHow To Optimize Your IMMUNOHISTOCHEMISTRY EXPERIMENT
How To Optimize Your IMMUNOHISTOCHEMISTRY EXPERIMENT www.ptglab.com 2 How To Optimize Your IHC Experiment ptglab.com 3 CONTENTS 4 5 Introduction To Immunohistochemistry 6 9 General Protocols 10 11 Antigen
More informationINOS. Colorimetric Cell-Based ELISA Kit. Catalog #: OKAG00807
INOS Colorimetric Cell-Based ELISA Kit Catalog #: OKAG00807 Please read the provided manual entirely prior to use as suggested experimental protocols may have changed. Research Purposes Only. Not Intended
More informationPhosphohistone-H3 (PHH3)
Rev. 0.0 v3 Phosphohistone-H3 (PHH3) Rabbit Polyclonal Antibody PRODUCT AVAILABILITY Cat. No. Description 369A-14 0.1 ml, concentrate 369A-15 0.5 ml, concentrate 369A-16 1.0 ml, concentrate 369A-17 1.0
More informationImmuLux Human IL-6 Fluorescent ELISA Kit
INSTRUCTIONS ImmuLux Human IL- Fluorescent ELISA Kit 3747 N. Meridian Road P.O. Box 7 Rockford, IL 05 840 53w Product Description Number Description 840 ImmuLux Human IL- Fluorescent ELISA Kit The Pierce
More informationThe following materials, sufficient for processing at least 50 slides, are included in the ISH Detection System K0601. Stringent Wash Concentrate
Dako In Situ Hybridization Detection System For Biotinylated Probes Code K0601 Intended use For In Vitro diagnostic use. The In situ Hybridization Detection System (K0601) is intended for the detection
More informationHuman IgG ELISA Quantitation Set
Human IgG ELISA Quantitation Set Cat. No. E80-104 Components Supplied Affinity purified Goat anti-human IgG-Fc Coating Antibody A80-104A, 1 ml at 1 mg/ml Human Reference Serum, RS10-110-4, 0.1 ml HRP Conjugated
More informationCytoGLOW. IKK-α/β. Colorimetric Cell-Based ELISA Kit. Catalog #: CB5358
CytoGLOW IKK-α/β Colorimetric Cell-Based ELISA Kit Catalog #: CB5358 Please read the provided manual entirely prior to use as suggested experimental protocols may have changed. Research Purposes Only.
More informationMouse Factor XII Total ELISA Kit
Mouse Factor XII Total ELISA Kit Catalog No: IMFXIIKT-TOT Lot No: SAMPLE INTENDED USE This mouse coagulation Factor XII antigen assay is intended for the quantitative determination of total Factor XII
More informationHuman Collagen Type III (COL3) ELISA
Human Collagen Type III (COL3) ELISA For the quantitative determination of human COL3 in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-61018 For Research Use Only. Not for use
More informationAnti-White Spot Syndrome Virus (WSSV) monoclonal antibody. Product no: P13
Anti-White Spot Syndrome Virus (WSSV) monoclonal antibody Product no: P13 Product Description The monoclonal antibody (Mab) against the Vp28 protein of White Spot Syndrome Virus (WSSV) is specific for
More informationHuman myelin basic protein(mbp) antibody ELISA Kit
Human myelin basic protein(mbp) antibody ELISA Kit Catalog Number.... For the quantitative determination of human myelin basic protein (MBP) antibody concentrations in serum, cerebrospinal fluid (CSF).
More informationMouse Collagen Type III ELISA
Mouse Collagen Type III ELISA Catalog Number M046062 For the quantitative determination of Collagen Type III in mouse plasma and serum samples. For research use only. This product insert must be read in
More informationPerkinElmer Life and Analytical Sciences, Inc.
PerkinElmer Life and Analytical Sciences, Inc. TSA PLUS FLUORESCENCE SYSTEMS Tyramide Signal Amplification For Fluorescence In situ Hybridization and Immunohistochemistry Fluorescein Tetramethylrhodamine
More informationEGFR (Phospho-Ser695)
Assay Biotechnology Company www.assaybiotech.com Tel: 1-877-883-7988 Fax: 1-877-610-9758 EGFR (Phospho-Ser695) Colorimetric Cell-Based ELISA Kit Catalog #: OKAG02090 Please read the provided manual entirely
More informationHuman IgG Antigen ELISA Kit
Human IgG Antigen ELISA Kit Catalog No: IHUIGGKT Lot No: SAMPLE INTENDED USE This human immunoglobulin G antigen assay is intended for the quantitative determination of total human IgG antigen in serum,
More informationMouse Peptide YY (PYY) ELISA
Mouse Peptide YY (PYY) ELISA For the quantitative determination of mouse PYY in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-58705 For Research Use Only. Not for use in diagnostic
More information1 MICROTITER PLATE 96 wells 2 ENZYME CONJUGATE 10.0 ml 1 vial 3 STANDARD.1 0 pg/ml 1 vial 4 STANDARD pg/ml 1 vial 5 STANDARD.
Rabbit Thyrotropin Releasing Hormone (TRH) Elisa Kit 96 Tests Catalog Number: MBS725153 Store all reagents at 2-8 C Valid Period:six months FOR LABORATORY RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC
More informationHuman Angiotensin 2 (Ang2) ELISA
Human Angiotensin 2 (Ang2) ELISA For the quantitative determination of human Ang2 in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-52748 For Research Use Only. Not for use in
More informationMouse Gonadotropin Releasing Hormone (GnRH) ELISA
KAMIYA BIOMEDICAL COMPANY Mouse Gonadotropin Releasing Hormone (GnRH) ELISA For the quantitative determination of mouse GnRH in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-58182
More informationRat Cluster of Differentiation 68 (CD68) ELISA kit. Catalog No. MBS (96 T)
Rat Cluster of Differentiation 68 (CD68) ELISA kit Catalog No. MBS705029 (96 T) This immunoassay kit allows for the in vitro quantitative determination of rat CD68 concentrations in serum, plasma and other
More informationHuman vascular endothelial cell growth factor A (VEGF-A) ELISA Kit
Human vascular endothelial cell growth factor A (VEGF-A) ELISA Kit For the quantitative determination of human vascular endothelial cell growth factor A (VEGF-A) concentrations in serum, plasma, tissue
More informationHuman anti-hepatitis A virus (HAV) antibody (IgM) ELISA Kit
Human anti-hepatitis A virus (HAV) antibody (IgM) ELISA Kit For the qualitative determination of human anti-hepatitis A virus (HAV) antibody (IgM) concentrations in serum. This package insert must be read
More informationHuman Amyloid Beta Peptide 1-42 (Aβ1-42) ELISA Kit
Human Amyloid Beta Peptide 1-42 (Aβ1-42) ELISA Kit Catalog Number. For the quantitative determination of human amyloid beta peptide 1-42 (Aβ1-42) concentrations in serum, plasma, tissue homogenates, cerebrospinal
More informationHuman rotavirus (RV) antibody (IgG) ELISA kit
Human rotavirus (RV) antibody (IgG) ELISA kit For the qualitative determination of human rotavirus (RV) antibody (IgG) concentrations in serum. This package insert must be read in its entirety before using
More informationCanine Symmetric dimethylarginine ELISA Kit
Distribuito in ITALIA da Li StarFish S.r.l. Via Cavour, 35 20063 Cernusco S/N (MI) telefono 02-92150794 fax 02-92157285 info@listarfish.it www.listarfish.it Optimize Your Research Canine Symmetric dimethylarginine
More informationHuman titin antibody(igg) ELISA Kit
Human titin antibody(igg) ELISA Kit Catalog Number. CSB-E13356h For the qualitative determination of human titin antibody(igg) concentrations in serum. This package insert must be read in its entirety
More informationRNAscope 2.5 LS Assay- RED Combined with Immunohistochemistry (IHC)
TECHNICAL NOTE RNAscope 2.5 LS Assay- RED Combined with Immunohistochemistry (IHC) Introduction This Technical Note provides guidelines for performing automated in situ hybridization (ISH) using an RNAscope
More informationImmunohistochemistry. How does it look like? When do we need IHC? When do we need IHC? In clinic: In research:
Introduction How does it look like? Immunohistochemistry Smooth muscle actin Parvalbumin Distrophyn Sandrine Bichet Head of Molecular Histology Platform Signal versus background 06.03.2012 IHC basics Introduction
More informationHuman helicobacter pylori antibody(iga) ELISA Kit
Human helicobacter pylori antibody(iga) ELISA Kit Catalog Number. CSB-E17034h For the qualitative determination of human helicobacter pylori antibody(iga) concentrations in serum. This package insert must
More informationRNAscope 2.5 LS Assay- BROWN Combined with Immunohistochemistry (IHC)
TECHNICAL NOTE RNAscope 2.5 LS Assay- BROWN Combined with Immunohistochemistry (IHC) Introduction This Technical Note provides guidelines for performing automated in situ hybridization (ISH) using an RNAscope
More informationHuman anti-toxoplasma (TOX) antibody (IgG) ELISA Kit
Human anti-toxoplasma (TOX) antibody (IgG) ELISA Kit Catalog Number. CSB-E09532h For the qualitative determination of human anti-tox antibody (IgG) concentrations in serum, plasma. This package insert
More informationHuman cross linked N-telopeptide of type I collagen (NTX) ELISA Kit
Human cross linked N-telopeptide of type I collagen (NTX) ELISA Kit Cat.No: DEIA4005 Lot. No. (See product label) Size 96T Intended use For the quantitative determination of human cross linked N-telopeptide
More informationBovine Prostaglandin E2 (PG-E2) ELISA Kit
Bovine Prostaglandin E2 (PG-E2) ELISA Kit Catalog Number. CSB-E14237B For the quantitative determination of endogenic bovine prostaglandin E2 (PG-E2) concentrations in serum, plasma, tissue homogenates.
More informationHuman Cluster of Differentiation 68 (CD68) ELISA Kit
Human Cluster of Differentiation 68 (CD68) ELISA Kit Catalog Number. CSB-E15979h For the quantitative determination of human cluster of differentiation 68 (CD68) concentrations in serum, plasma, tissue
More informationNovocastra Liquid Mouse Monoclonal Antibody Nitric Oxide Synthase-1
Novocastra Liquid Mouse Monoclonal Antibody Nitric Oxide Synthase-1 Product Code: NCL-L-NOS-1 Leica Biosystems Newcastle Ltd Balliol Business Park West Benton Lane Newcastle Upon Tyne NE12 8EW United Kingdom
More informationLSBio TM Mouse/Human/Rat CAMK2B / CaMKII Beta Cell-Based ELISA Kit. Catalog No. LS-F1847. User Manual
LSBio TM Mouse/Human/Rat CAMK2B / CaMKII Beta Cell-Based ELISA Kit Catalog No. LS-F1847 User Manual Please Read the Manual Carefully Before Starting your Experiment For research use only. Not approved
More informationHAM-56 antibody reacts with monocytes, but is unreactive with B and T-lymphocytes. Principles And Procedures
Product Identification Cat. No. Description 44682 Macrophage Marker 0,1 M (HAM- 56) 44683 Macrophage Marker 1 M (HAM-56) 44330 Macrophage Marker RTU M (HAM- 56) Symbol Definitions P C A E S DIL DOC# DIS
More informationLSBio TM Mouse/Human/Rat Phospho-SMAD3 Cell-Based Phosphorylation ELISA Kit. Catalog No. LS-F1058. User Manual
LSBio TM Mouse/Human/Rat Phospho-SMAD3 Cell-Based Phosphorylation ELISA Kit Catalog No. LS-F1058 User Manual Please Read the Manual Carefully Before Starting your Experiment For research use only. Not
More informationHuman IgG ELISA Kit. Strip well format. Reagents for up to 96 tests
Human IgG ELISA Kit Strip well format. Reagents for up to 96 tests Catalog No. CS222A Quantity: 1 x 96 tests CS222B 5 x 96 tests Intended Use: Background: Assay Principle: This human immunoglobulin G antigen
More informationHuman IL-10 ELISA MAX Set Deluxe
Human IL-10 ELISA MAX Set Deluxe Cat. No. 430604 (5 plates) 430605 (10 plates) 430606 (20 plates) ELISA Set for Accurate Cytokine Quantification from Cell Culture Supernatant, Serum, Plasma or Other Body
More informationActin, Muscle Specific (HHF35)
Product Identification Cat. No. Description 45235 IMPATH Actin Muscle Specific RTU M (HHF35) Symbol Definitions P A E S DOC# DIS ready-to-use ascites serum supernatant document number distributed by Intended
More informationHuman connective tissue growth factor (CTGF) ELISA Kit. MyBioSource.com. This package insert must be read in its entirety before using this product.
Human connective tissue growth factor (CTGF) ELISA Kit Catalog Number. For the quantitative determination of human connective tissue growth factor (CTGF) concentrations in serum, plasma, tissue homogenates.
More informationab In situ Apoptosis Detection Kit
ab206386 In situ Apoptosis Detection Kit Instructions for Use For detection of apoptotic cells. This product is for research use only and is not intended for diagnostic use. Version 8 Last Updated 6 February
More informationPrinciples And Procedures
Product Identification Cat. No. Description 44837 Vimentin 0,1 M (V9) 44838 Vimentin 1 M (V9) 44409 Vimentin RTU M (V9) Symbol Definitions P C A E S DIL DOC# DIS ready-to-use concentrate ascites serum
More informationHistology FISH Accessory Kit Step-by-Step Procedure
PROCEDURE Histology FISH Accessory Kit Step-by-Step Procedure Code K5799 For probes diluted in Formamide based hybridization buffer Reagent Preparation Equilibration Deparaffinization/Rehydration Prepare
More informationTranscription Factor ELISA Kit
Transcription Factor ELISA Kit User Manual P/N 12490 Rev. A 061119 DRAFT November 19, 2006 6:40 pm ElisaTFTtile.fm Panomics, Inc. Transcription Factor ELISA Kit User Manual Copyright Copyright 2006, Panomics,
More informationMyBioSource.com. Human Total Bilirubin (TBB) Elisa kit. (Competitive ELISA)
Human Total Bilirubin (TBB) Elisa kit (Competitive ELISA) 96 Tests Catalog Number: MBS756198 Store all reagents at 2-8 C Valid Period: six months For samples: Serum, plasma, cell culture supernatants,
More informationHP EASE. Qualitative assay for IgG antibodies to Helicobacter pylori Product code GD21 (96 tests)
HP EASE Qualitative assay for IgG antibodies to Helicobacter pylori Product code GD21 (96 tests) For Research Use Only. Not For Use In Diagnostic Procedures. Updates from previous versions are non-critical
More informationActin, Smooth Muscle (1A4)
Product Identification Cat. No. Description 45121 IMPATH Actin Smooth Muscle RTU M (1A4) Symbol Definitions P A E S DOC# DIS ready-to-use ascites serum supernatant document number distributed by Intended
More informationMouse Apolipoprotein A1 (Apo-A1) ELISA Kit
Mouse Apolipoprotein A1 (Apo-A1) ELISA Kit Catalog Number. CSB-E08104m For the quantitative determination of mouse apolipoprotein A1 (Apo-A1) concentrations in serum, plasma, cell culture supernates, tissue
More informationLSBio TM Human Anti-Chlamydia pneumoniae antibody (IgG) Direct ELISA Kit. Catalog No. LS-F User Manual
LSBio TM Human Anti-Chlamydia pneumoniae antibody (IgG) Direct ELISA Kit Catalog No. LS-F10326 User Manual Please Read the Manual Carefully Before Starting your Experiment For research use only. Not approved
More informationTransferrin ELISA. For the quantitative determination of transferrin in human biological samples
Transferrin ELISA For the quantitative determination of transferrin in human biological samples Please see Appendix A for Reference Serum information. For Research Use Only. Not For Use In Diagnostic Procedures.
More informationHuman IgG Rubella ELISA Kit
Human IgG Rubella ELISA Kit Catalog No: IRAPKT2020 Lot No: SAMPLE INTENDED USE The Rubella IgG ELISA is intended for use in evaluating a patient s serologic status to the rubella virus infection. It is
More informationRat Creatinine (Cr) ELISA Kit
Rat Creatinine (Cr) ELISA Kit Catalog Number. CSB-E16687r For the quantitative determination of endogenic rat creatinine (Cr) concentrations in serum, plasma, cell culture supernates, tissue homogenates
More informationFibrinogen ELISA. For the quantitative determination of fibrinogen in biological fluids, serum, and plasma.
Fibrinogen ELISA For the quantitative determination of fibrinogen in biological fluids, serum, and plasma. Please read carefully due to Critical Changes, e.g., Changes to preparation of standard volume
More informationOpal 4 Lymphocyte Kit
Manual Multiplex IHC Research Use Only. Not for use in diagnostic procedures. Opal 4 Lymphocyte Kit Product Information Storage Stability Application Species Reactivity Safety Note Quality Control Store
More informationInstructions for use
Instructions for use SARCOPTES-ELISA 2001 DOG Test to detect the presence of antibodies against the cause of mange of dogs, Sarcoptes scabiei var. canis In-vitro Diagnostic for Dog Contents of the Kit:
More informationAnti-Human IDH1 R132H Astrocytoma, Oligodendroglioma Tumor Cell Marker Mouse Monoclonal Antibody Clone H09
1 of 5 Technical Note 1 Procedure: Automated Immunostaining Ventana Benchmark XT XT ultraview DAB DIA--M (20µg) Reconstitution: DIA- (100µg), restore to 500µl Summary 1. Cut sections to 4 µm (Microm HM
More informationHuman Plasmin Activity Assay ELISA Kit
Human Plasmin Activity Assay ELISA Kit Catalog No. CSI12527A 1 x 96 tests CSI12527B 5 x 96 tests Intended Use: Background: Assay Principle: Reagents Provided: This human plasmin activity assay is for the
More informationPorcine IL-12/IL-23 p40 ELISA kit
Porcine IL-12/IL-23 p40 ELISA kit Catalog number: NB-E50014 (96 wells) The kit is designed to quantitatively detect the levels of Porcine IL-12/IL-23 p40 in cell culture supernatants. FOR RESEARCH USE
More informationHiPer Sandwich ELISA Teaching Kit
HiPer Sandwich ELISA Teaching Kit Product Code: HTI014 Number of experiments that can be performed: 4 Duration of Experiment: 2 days Day1-Coating of wells: 15 minutes Day2- protocol, observation and result:
More informationRat TNF-α ELISA REF. Ver5.0 RUO. ELISA Set for Accurate Quantitation from Cell Culture Supernatant, Serum, Plasma or Other Bodily Fluids
REF : KB3145 Ver5.0 RUO ELISA Set for Accurate Quantitation from Cell Culture Supernatant, Serum, Plasma or Other Bodily Fluids RUO For Research Use Only REF Catalog Number Store At Manufactured By Expiry
More informationHuman IL-6 ELISA Set
Human IL-6 ELISA Set Catalog No. CDK082B Quantity: 10 x 96 tests PRODUCT SPECIFICATIONS : Specificity: Recognizes both natural and recombinant human IL-6 Range: 6.25 pg / ml - 200 pg / ml Sensitivity:
More informationHuman placenta lactogen (HPL) ELISA Kit
Human placenta lactogen (HPL) ELISA Kit Catalog Number. CSB-E09665h For the quantitative determination of human placenta lactogen (HPL) concentrations in serum, plasma. This package insert must be read
More informationDepartment of Microbiology, Lab 016 instructions
Protocol for standard FISH and DOPE-FISH for prokaryotes (slightly modified from Amann, 1995, note, for other modifications or other microorganisms like eukaryotes, consult special literature or check
More informationDIAGNOSTIC AUTOMATION, INC.
DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com MATERIAL
More informationChikungunya IgM ELISA Kit
Chikungunya IgM ELISA Kit Cat.No: DEIA2163 Lot. No. (See product label) Intended Use The Chikungunya IgM-μ-capture ELISA is intended for measurement of IgM class antibodies to Chikungunya virus in human
More informationImmunoassay Kit Catalog # KCA0021. Canine. C-Reactive Protein
Immunoassay Kit Catalog # KCA0021 Canine C-Reactive Protein BioSource International, Inc. 542 Flynn Road Camarillo, California 93012 USA Tel: 805-987-0086 800-242-0607 FAX: 805-987-3385 email: tech.support@biosource.com
More informationPeliClass human IgG subclass ELISA kit Enzyme-linked immunosorbent assay
PeliClass human IgG subclass ELISA kit Enzyme-linked immunosorbent assay Catalog No: M1551 Size: six pre-coated 8-well strips for each of the four IgG subclasses Test description The PeliClass human subclass
More informationIgG Antibodies To Toxoplasma. Gondii ELISA Kit
IgG Antibodies To Toxoplasma Catalog No: IRAPKT1405 Gondii ELISA Kit Lot No: SAMPLE INTENDED USE The Toxoplasma IgG ELISA is intended for use in evaluating a patient s serologic status to Toxoplasma gondii
More informationMouse Fibrinogen ELISA
K-ASSAY Mouse Fibrinogen ELISA For the quantitative determination of fibrinogen in mouse biological samples Cat. No. KT-397 For research use only. 1 Rev. 9994397 K-ASSAY PRODUCT INFORMATION Mouse Fibrinogen
More informationHuman VEGF-C ELISA. For the precise measurement of VEGF-C in human serum, plasma, body fluids, tissue homogenate or cell culture supernates.
Product information User s Manual Human VEGF-C ELISA For the precise measurement of VEGF-C in human serum, plasma, body fluids, tissue homogenate or cell culture supernates. BE69217 Storage: 96 2-8 C RUO
More informationGenUP Virus DNA/RNA Kit
Product Insert GenUP Virus DNA/RNA Kit LOT: See product label EXPIRY DATE: See product label ORDERING INFORMATION PRODUCT GenUP Virus DNA/RNA Kit CAT. NO. BR0701101 BR0701102 BR0701103 SIZE 10 preps 50
More informationKPL SignaLOCK ChemiWestern Kits (Film and Imager Analysis)
KPL SignaLOCK ChemiWestern Kits (Film and Imager Analysis) SignaLOCK HRP ChemiWestern Kit (Film) Catalog No. 54-53-00 SignaLOCK HRP ChemiWestern Kit (Imager) Catalog No. 54-54-00 SignaLOCK AP ChemiWestern
More informationRat Calcitonin Gene Related Peptide (CGRP) Elisa kit
Rat Calcitonin Gene Related Peptide (CGRP) Elisa kit 96 Tests Catalogue Number: AMS.E02C0744 Store all reagents at 2-8 C Valid Period: six months For samples: Serum, plasma, cell culture supernatants,
More information