Chapter 13 - Regulation of Gene Expression
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1 Chapter 13 - Regulation of Gene Expression 1. Describe the typical components of an operon in an E. coli (prokaryotic) cell. (p ) a. regulator gene - b. promoter - c. operator - d. structural gene - 2. Jacob and Monod studied two types of E. coli operons, the operon and the operon. (p.239) 3. Think of the operon as a series of switches that are turned on and off depending on the immediate needs of the bacterium. The trp operon exists like this in the on position. For E. coli, it periodically needs the amino acid tryptophan to perform certain functions. When enough tryptophan is produced, the amino acid shuts off the process by binding to an ceasing the producing of the structural genes and, consequently, the needed to break it down. Sketch figure 13.1 and summarize the trp operon in the presence and absence of tryptophan. Tryptophan absent Tryptophan present 1 of 8
2 a. summary of tryptophan absent - b. summary of tryptophan present - 4. Describe the 3 genes that encode the enzymes needed to break down lactose in bacteria. (p. 239) 5. Examine figure Notice how the lac operon is continuously off. The is perpetually bound to the operator which prevents the from binding to the promotor. As a result, the genes cannot be transcribed into mrna and consequently cannot express the enzymes to break down. However, when lactose is present, it binds to the which prevents the the from binding to the operator. That leaves RNA polymerase free to bind to the, facilitating the transcription of lactose metabolizing genes and the enzymes needed to break down. lactose present lactose absent 2 of 8
3 a. summary of lactose present - b. summary of present absent - 6. Describe how lactose is considered an inducer of the lac operon. (p. 240) 7. Fill in the matrix detailing the different mechanisms in eukaryotic cells that can modify genes (p. 241) Mechanism Description transcriptional control used in a way to keep genes turned off. Chromatin structure is one method of epigenetic inheritance which is the transmission of genetic information outside the coding sequences of a gene. translational control takes place in the cytoplasm occurs after protein synthesis. Only a functional protein is an active gene product. posttranscriptional control 3 of 8
4 8. Define the term chromatin. Where is it most evident? (p. 241) 9. Differentiate between euchromatin and heterochromatin. (p. 242) 10. What type of histone tail does heterochromatin contain?. Euchromatin? 11. When does euchromatin become genetically active? (p. 242) 12. Summarize figure 13.5 b and c. Be sure to describe how DNA becomes free for transcription and the molecules involved. b. c. 13. Why do only females contain a barr body? How does a barr body prevent females from expressing more genes than males? (p. 243) 4 of 8
5 14. This goes against everything I ve taught you about genetics, but there is way for females who are heterozygous for an X-linked trait to express the recessive AND dominant allele in different cells!! This is achieved by X-inactivation which produces a barr body. Take a look for yourself by summarizing figure 13.6! 15. What does the term epigenetic inheritance generally refer to? Provide an example. (p. 243) 16. What is the role of a transcription factor in a eukaryotic cell? (p. 244) 5 of 8
6 MR. HAYES AP BIOLOGY 17. Fill in the matrix regarding transcriptional control. (p. 244) Component Description transcription factor transcription activator enhancer 18. Transcription in eukaryotic cells requires to bind to the promoter and the to bind to the enhancer. The enhancer may be far from the promoter, so the DNA loops over itself so the transcription factor can bind to the transcription activator via. 19. Where does post transcriptional control occur and what does it include? (p. 244) 20. What is the main benefit of alternative pre-mrna splicing in humans? (p. 244) 6 of 8
7 21. What is implied about the proteins made from identical mrna strands that have undergone independent pre-mrna splicing? (figure 13.8) 22. Scientists originally thought that 99% of DNA was junk because it didn t code for specific proteins. Now we know that this DNA code for a specific type of RNA called. 23. Describe 3 ways in which srna can regulate gene expression. (p ) 1.) 2.) 3.) 24. Define a gene mutation. (p. 247) 7 of 8
8 MR. HAYES AP BIOLOGY 25. Fill in the matrix of the different causes of gene mutations. (p ) Mutation Description Example spontaneous induced point frameshift 26. How do proto-oncogenes and tumor suppressor genes (in conjunction with transcription factors) contribute to the development of cancer? (p. 249) 27. Summarize the two cell signaling pathways below. (figure and 13.14) 8 of 8
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