CET VIKASANA PROGRAMME 2013 MOLECULAR BIOLOGY
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1 CET VIKASANA PROGRAMME 2013 MOLECULAR BIOLOGY
2 Molecular biology is the study of molecular underpinnings of the process of replication, transcription and translation of the genetic material.
3 DNA RNA Protein
4 DNA Two long strands makes the shape of a double helix. two strands run in opposite directions to each other and are therefore antiparallel. Chemically, DNA consists of two long polymers of simple units called nucleotides, with backbones made of base, sugars and phosphate groups.
5 RNA is a biologically important type of molecule that consists of a long chain of nucleotide units. Each nucleotide consists of a nitrogenous base, a ribose sugar, and a phosphate. Ribonucleic acid (RNA)
6 Difference between RNA & DNA RNA RNA nucleotides contain ribose sugar RNA has the base uracil presence of a hydroxyl group at the 2' position of the ribose sugar. RNA is usually singlestranded DNA DNA contains deoxyribose DNA has the base thymine Lacks of a hydroxyl group at the 2' position of the ribose sugar. DNA is usually doublestranded
7 DNA replication DNA replication, the basis for biological inheritance, is a fundamental process occurring in all living organisms to copy their DNA. In the process of "replication" each strand of the original double-stranded DNA molecule serves as template for the reproduction of the complementary strand. Two identical DNA molecules have been produced from a single double-stranded DNA molecule.
8 CENTRAL DOGMA OF MOLECULAR BIOLOGY
9 Genetic code
10 BIOLOGY Double helical model was proposed by a. Boysen and Jensen b. Watson and Crick c. Watson and Tatum d. Schleiden and Schwann
11 Double helical model was proposed by a. Boysen and Jensen b. Watson and Crick c. Watson and Tatum d. Schleiden and Schwann
12 BIOLOGY Who among the following conclusively proved that DNA is the genetic material? a. O.T Avery. C. Macleod and McCarty b. Meselson and Stahl c. Hargobind Khorana. Holley and Nirenberg d. Tatum and Lederberg
13 BIOLOGY Who among the following conclusively proved that DNA is the genetic material? a. O.T Avery. C. Macleod and McCarty b. Meselson and Stahl c. Hargobind Khorana. Holley and Nirenberg d. Tatum and Lederberg
14 BIOLOGY A nucleotide consists of a. A nitrogen base and pentose sugar. b. A nitrogen base and phosphate c. A Pentose sugar and phosphate d. A nitrogen base, pentose sugar & phosphate
15 BIOLOGY A nucleotide consists of a. A nitrogen base and pentose sugar. b. A nitrogen base and phosphate c. A Pentose sugar and phosphate d. A nitrogen base, pentose sugar & phosphate
16
17 Bacterial transformation was discovered by: a. Avery et al b. Watson and Crick c. Griffith d. Hershey and Chase
18 Bacterial transformation was discovered by: a. Avery et al b. Watson and Crick c. Griffith d. Hershey and Chase
19 RNA contains the following sugar: a. Glucose b. Ribose c. Hexose d. Fructose
20 RNA contains the following sugar: a. Glucose b. Ribose c. Hexose d. Fructose
21 DNA is a polymer of: a. Proteins b. RNA c. Carbohydrates d. Nucleotides
22 DNA is a polymer of: a. Proteins b. RNA c. Carbohydrates d. Nucleotides
23 All of the following elements are present in DNA except: a. Phosphorous b. Carbon c. Sulphur d. Nitrogen
24 All of the following elements are present in DNA except: a. Phosphorous b. Carbon c. Sulphur d. Nitrogen
25 The four nitrogenous bases found in DNA which forms its language are: a. UTAC b. ACTU c. AGTU d. ATCG
26 The four nitrogenous bases found in DNA which forms its language are: a. UTAC b. ACTU c. AGTU d. ATCG
27 The base that is not found in DNA but found in RNA is: a. Thymine b. Uracil c. Adenine d. Guanine
28 The base that is not found in DNA but found in RNA is: a. Thymine b. Uracil c. Adenine d. Guanine
29 Which purine is found in RNA? a.guanine b. Cytosine c. Thymine d. Uracil
30 Which purine is found in RNA? a.guanine b. Cytosine c. Thymine d. Uracil
31 Which sequence has four pyrimidines? a. CATCAATGG b. UAGCGGUAA c. TGGATAACG d. GCUAGACAA
32 Which sequence has four pyrimidines? a. CATCAATGG b. UAGCGGUAA c. TGGATAACG d. GCUAGACAA
33 DNA consists of two complimentary chains of nucleotides. If the sequence of nucleotides in one chain is AGCTTCGA, then the sequence in the other chain is a.taggatat b. GATCCTAG c. TCGAAGCT d. GCTAAGCT
34 DNA consists of two complimentary chains of nucleotides. If the sequence of nucleotides in one chain is AGCTTCGA, then the sequence in the other chain is a.taggatat b. GATCCTAG c. TCGAAGCT d. GCTAAGCT
35 Which of the following is made up of single ring of atoms? a. Alanine b. Adenine c. Guanine d. Thymine
36 Which of the following is made up of single ring of atoms? a. Alanine b. Adenine c. Guanine d. Thymine
37 The two strands of DNA are: a. Similar and parallel b. Similar and antiparallel c. Complementary and antiparallel d. Complementary and parallel
38 The two strands of DNA are: a. Similar and parallel b. Similar and antiparallel c. Complementary and antiparallel d. Complementary and parallel
39 5' C-G-A-T-T-G-C-A-A-C-G-A-T-G-C 3' 3' G-C-T-A-A-C-G-T-T-G-C-T-A-C-G 5'
40 DNA acts as a template for: a. Proteins b. DNA c. RNA d. Both DNA and RNA
41 DNA acts as a template for: a. Proteins b. DNA c. RNA d. Both DNA and RNA
42 Formation of RNA from DNA is called: a. Transcription b. Translation c. Replication d. Recombination
43 Formation of RNA from DNA is called: a. Transcription b. Translation c. Replication d. Recombination
44 TRANSCRIPTION (DNA-RNA)
45 Inheritable gene mutation takes place in a. Nuclear DNA b. Mitochondrial DNA c. Chloroplast DNA d. All the above
46 Inheritable gene mutation takes place in a. Nuclear DNA b. Mitochondrial DNA c. Chloroplast DNA d. All the above
47 mrna is synthesized on DNA in which direction: a. 5' 3' b. 3' 5' c. 5' 3' and 3' 5' d. 3' 5' and 5' 3'
48 mrna is synthesized on DNA in which direction: a. 5' 3' b. 3' 5' c. 5' 3' and 3' 5' d. 3' 5' and 5' 3'
49 Split genes were discovered by a. Watson and Crick b. Lederberg and Tatum c. Jacob and Monad d. Sharp and Roberts
50 Split genes were discovered by a. Watson and Crick b. Lederberg and Tatum c. Jacob and Monad d. Sharp and Roberts
51
52 Molecule into which the coded information is transcribed is: a. mrna b. trna c. rrna d. hnrna
53 Molecule into which the coded information is transcribed is: a. mrna b. trna c. rrna d. hnrna
54 The two strands of DNA are held together by bonds: a. Nitrogen b. Hydrogen c. Oxygen d. Carbon
55 The two strands of DNA are held together by bonds: a. Nitrogen b. Hydrogen c. Oxygen d. Carbon
56 Hydrogen bonds present between Cytosine and Guanine are: a. 2 b. 3 c. 1 d. 4
57 Hydrogen bonds present between Cytosine and Guanine are: a. 2 b. 3 c. 1 d. 4
58 The ratio of purine and pyrimidine bases in a DNA molecule is always around one. This is known as: a. Wobble hypothesis b. Teminism c. Chargaff's rule d. Colinearity
59 The ratio of purine and pyrimidine bases in a DNA molecule is always around one. This is known as: a. Wobble hypothesis b. Teminism c. Chargaff's rule d. Colinearity
60 In a double stranded DNA molecule, the percentage of Cytosine is 18 what is the percent of Adenine? a. 64% b. 32% c. 18% d. 46%
61 In a double stranded DNA molecule, the percentage of Cytosine is 18 what is the percent of Adenine? a. 64% b. 32% c. 18% d. 46%
62 A short length of DNA molecule contains 120 Adenine and 120 Cytosine bases. The total number of nucleotides in this DNA segment is: a. 60 b. 120 c. 240 d. 480
63 A short length of DNA molecule contains 120 Adenine and 120 Cytosine bases. The total number of nucleotides in this DNA segment is: a. 60 b. 120 c. 240 d. 480
64 The replication of nuclear DNA occurs in: a. G1 - phase b. G2 - phase c. S - phase d. M - phase
65 The replication of nuclear DNA occurs in: a. G1 - phase b. G2 - phase c. S - phase d. M - phase
66
67 The process of multiplication of DNA from DNA is known as: a. Replication b. Mutation c. Transcription d. Translation
68 The process of multiplication of DNA from DNA is known as: a. Replication b. Mutation c. Transcription d. Translation
69 REPLICATION
70 The term triplet code and genetic code were proposed by: a. Watson and Crick b. Nirenberg c. Gamow d. Friederich Meischer Molecular Biology
71 The term triplet code and genetic code were proposed by: a. Watson and Crick b. Nirenberg c. Gamow d. Friederich Meischer Molecular Biology
72 Match the nucleotide triplets given in column 1 and their common names listed in column 2, choose the answer with correct combination of alphabets of the 2 columns Column 1(stop codon) Column 2 (function) A UAA UAG UGA p Amber codon B UAA q Initiatior codon C UAG r Ochre codon D AUG s Terminator codon
73 Match the nucleotide triplets given in column 1 and their common names listed in column 2, choose the answer with correct combination of alphabets of the 2 columns Column1(stop codon) Column2(function) A UAA,UAG,UGA p Amber codon B UAA q Initiator codon C UAG r Ochre codon D AUG s Terminator codon
74 Information flow or central dogma of molecular biology is: a. RNA Proteins DNA b. Proteins DNA RNA c. RNA DNA Proteins d. DNA RNA Proteins
75 Information flow or central dogma of molecular biology is: a. RNA Proteins DNA b. Proteins DNA RNA c. RNA DNA Proteins d. DNA RNA Proteins
76
77 Removal of introns and joining the exons in a defined order in a transcription unit is called: a. Tailing b. Transformation c. Capping d. Splicing
78 Removal of introns and joining the exons in a defined order in a transcription unit is called: a. Tailing b. Transformation c. Capping d. Splicing
79 Number of punctuation codons are: a. 4 b. 2 c. 1 d. 5
80 Number of punctuation codons are: a. 4 b. 2 c. 1 d. 5
81 Genetic code is degenerate because: a. codons have same energy level b.each codon has a different meaning c. Each codon has many meanings d. Many codons have same meaning
82 Genetic code is degenerate because: a. codons have same energy level b.each codon has a different meaning c. Each codon has many meanings d. Many codons have same meaning
83 DNA polymerase takes part in: a. Transcription b. Splicing c. Replication d. Teminism
84 DNA polymerase takes part in: a. Transcription b. Splicing c. Replication d. Teminism
85 The genes that keep changing their location on chromosomes are: a. Jumping genes b. Split genes c. Duplicate genes d. Pleiotropic genes
86 The genes that keep changing their location on chromosomes are: a. Jumping genes b. Split genes c. Duplicate genes d. Pleiotropic genes
87 Number of base pairs in each turn of ZDNA helix is: a. 10 b. 11 c. 12 d. 15
88 Number of base pairs in each turn of ZDNA helix is: a. 10 b. 11 c. 12 d. 15
89 Continuous DNA synthesis occurs during replication in: a. The leading strand b. The lagging strand c. The strands where Okazaki fragments are formed d. Both leading and lagging strands
90 Continuous DNA synthesis occurs during replication in: a. The leading strand b. The lagging strand c. The strands where Okazaki fragments are formed d. Both leading and lagging strands
91 Eukaryotic mrna has: a. G cap nucleotide b. Poly A tail c. Both G cap and poly A tail d. Saturated fats
92 Eukaryotic mrna has: a. G cap nucleotide b. Poly A tail c. Both G cap and poly A tail d. Saturated fats
93 Teminism is: a. Translation b. Transcription c. Reverse transcription d. Transduction
94 Teminism is: a. Translation b. Transcription c. Reverse transcription d. Transduction
95 Central dogma in protein synthesis of teminious organisms is: a. grna DNA mrna Proteins b. DNA DNA mrna Proteins c. mrna grna DNA Proteins d. DNA grna mrna Proteins
96 Central dogma in protein synthesis of teminious organisms is: a. grna DNA mrna Proteins b. DNA DNA mrna Proteins c. mrna grna DNA Proteins d. DNA grna mrna Proteins
97 The segment of DNA which participates in crossing over is: a. Muton b. Recon c. Cistron d. Replicon
98 The segment of DNA which participates in crossing over is: a. Muton b. Recon c. Cistron d. Replicon
99 What is true about ori? a. One in all organisms b. Several in all organisms c.one in eukaryotes and several in prokaryotes d.one in prokaryotes and several in eukaryotes
100 What is true about ori? a. One in all organisms b. Several in all organisms c.one in eukaryotes and several in prokaryotes d.one in prokaryotes and several in eukaryotes
101 Codogen is triplet of: a. Template strand of DNA b. Non-template strand of DNA c. mrna d. trna
102 Codogen is triplet of: a. Template strand of DNA b. Non-template strand of DNA c. mrna d. trna
103 Anticodons occur in: a. trna b. mrna c. mtdna d. rrna
104 Anticodons occur in: a. trna b. mrna c. mtdna d. rrna
105
106 Exons and Introns are present in: a. Prokaryotic mrna b. Eukaryotic mrna c. The Lac operon d. Mt RNA
107 Exons and Introns are present in: a. Prokaryotic mrna b. Eukaryotic mrna c. The Lac operon d. Mt RNA
108 Okazaki fragments give rise to: a. Master strand b. Sense strand c. Lagging strand d. Leading strand
109 Okazaki fragments give rise to: a. Master strand b. Sense strand c. Lagging strand d. Leading strand
110 Which RNA is called soluble RNA: a. trna b. mrna c. rrna d. snrna
111 BIOLOGY Which RNA is called soluble RNA: a. trna b. mrna c. rrna d. snrna
112 BIOLOGY Sequence of structural genes in lac operon concept is: a. Lac Y, Lac Z and Lac A b. Lac Z, Lac Y, Lac A c. Lac A, Lac Y, Lac Z d. Lac A, Lac Z, Lac Y
113 Sequence of structural genes in lac operon concept is: a. Lac Y, Lac Z and Lac A b. Lac Z, Lac Y, Lac A c. Lac A, Lac Y, Lac Z d. Lac A, Lac Z, Lac Y
114 The operator gene of Lac operon is turned on when inducer molecule binds to: a. Promoter site b. Operator gene c. mrna d. Repressor
115 BIOLOGY The operator gene of Lac operon is turned on when inducer molecule binds to: a. Promoter site b. Operator gene c. mrna d. Repressor
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