Thebiotutor.com A2 Biology OCR Unit F215: Control, genomes and environment Module 1.1 Cellular control Answers
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1 Thebiotutor.com A2 Biology OCR Unit F215: Control, genomes and environment Module 1.1 Cellular control Answers Andy Todd 1
2 1. 1 ref to operon; 2 normally repressor substance bound to operator; 3 prevents RNA polymerase binding (at promoter) / prevents transcription; 4 lactose binds to repressor; 5 changes shape of protein molecule; 6 unable to bind (to operator); 7 RNA polymerase binds (at promoter) / transcription occurs / genes switched on; 8 AVP; e.g. production of lactose permease / production of betagalactosidase; Max 5 [5] 2. a change in the genetic material; unpredictable / AW; extra detail; e.g. addition / substitution / deletion / frame shift / small part of chromosome / may code for different protein / may code for no protein [2] 3. allow max 5 for following: transcription; DNA unzips / H bonds break; exposing required, gene / sequence of bases; RNA nucleotides align with DNA; U with A, A with T, C with G, and G with C; RNA polymerase; mrna formed (using DNA strand as template); leaves nucleus through pore; allow max 5 for following: translation; mrna attaches to ribosome; trna brings amino acid (to, ribosome / mrna); each trna attached to specific amino acid; trna binds to mrna using complementary, base triplet / anticodon; peptide bond formed between amino acids; DNA / mrna, (nucleotide / base) sequence determines sequence of amino acids; AVP; e.g. 2, base triplets / codons, in ribosome AVP; e.g. ref. to : start / stop, codons polysomes large and small subunit in ribosome Mg 2+ [10] Andy Todd 2
3 4. 1 mark per correct row Look for both ticks and crosses. If a table consists of ticks ONLY or crosses ONLY, then assume that the blank spaces are the other symbol. If a table consists of ticks, crosses and blanks then the blanks represent no attempt at the answer. Nucleotides line up along an exposed DNA strand. ; The whole of the double helix unzips. ; Uracil pairs with adenine. ; A trna triplet pairs with an exposed codon. ; Both DNA polynucleotide chains act as templates. ; Adjacent nucleotides bond, forming a sugar-phosphate backbone. ; The original DNA molecule is unchanged after the process. ; Adenine pairs with thymine. ; [8] 5. (a) (i) U A C C G G A U U C A C;; 1 error = 1, 2 errors = 0 allow 1 mark for giving T throughout instead of U (i.e. T A C C G G A T T C A C = 1 mark) 2 (ii) transcription / transcribed; R transcriptase 1 (b) (i) J anticodon; R anticodons K transfer RNA / trna; L ribosome / rrna; M codon; R codons 4 Andy Todd 3
4 (ii) 1 DNA triplet / codon / M / mrna triplet, codes for specific amino acid; 2 order of, triplets / bases, determines the order of amino acids; 3 trna / K, has, corresponding / complementary, triplet / anticodon; 4 (trna / K) attached to specific amino acid; 5 activation of amino acid; 6 2 (trna) binding sites on the ribosome; 7 codon and anticodon bind; A match 8 A to U and C to G; 9 adjacent amino acids join; 10 peptide bond; 4 max 6. (c) 1 attaches to ribosome; 2 removes, base / portion, of ribosome; A stops ribosome assembling / changes shape of ribosome 3 prevents ribosome, attaching to / reading, mrna; 4 prevents codons being exposed; 5 prevents, trna / anticodon, attaching to, mrna / codon; 6 prevents / inhibits enzyme responsible for, formation of peptide linkages; 7 AVP; e.g. further detail of any of the above points (a) (i) mrna leaves nucleus; ora mrna, translated / used to make, protein; DNA, transcribed / used to make, mrna; mrna short-term / DNA (long-term) store; 2 max 2 max [13] (ii) sirna smaller / fewer nucleotides / only matches part of gene; ora sirna double-stranded; ora 2 Andy Todd 4
5 (b) (complementary) base-pairing; hydrogen bonding; between purines and pyrimidines; A with U; R A with T C with G; ref to 2 or 3 bonds (correct context); 3 max [7] 7. (a) provides sites for binding; ref to, spindle fibres / microtubules; ref to genes being spaced out along chromosome; places to break and rejoin (during meiotic division); A chiasmata formation junk implies no, function / purpose; ora function may not yet have been discovered; AVP; e.g. raw material for, evolution / natural selection, required for, cell division / mitosis / meiosis max 2 (b) straight line sloping up from left to right; (does not need to start at origin) 1 (c) ATP / NAD / NADP / RNA / phospholipid / GP / TP / RuBP / ADP / RUP / AMP / camp/ phosphocreatine / AVP; R DNA 1 [4] Andy Todd 5
PMT. 4. allow max 5 for following:
1. 1 ref to operon; 2 normally repressor substance bound to operator; 3 prevents RNA polymerase binding (at promoter) / prevents transcription; 4 lactose binds to repressor; 5 changes shape of protein
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