DNA Structure and Function. Chapter 13
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1 DNA Structure and Function Chapter 13
2 Miescher Discovered DNA 1868 Johann Miescher investigated the chemical composition of the nucleus Isolated an organic acid that was high in phosphorus He called it nuclein We call it DNA (deoxyribonucleic acid)
3 Mystery of the Hereditary Material Originally believed to be an unknown class of proteins Thinking was Heritable traits are diverse Molecules encoding traits must be diverse Proteins are made of 20 amino acids and are structurally diverse
4 Structure of the Hereditary Material Experiments in the 1950s showed that DNA is the hereditary material Scientists raced to determine the structure of DNA Watson and Crick proposed that DNA is a double helix Figure 13.2 Page 217
5 1928 Griffith Discovers Transformation Attempting to develop a vaccine Isolated two strains of Streptococcus pneumoniae Rough strain was harmless Smooth strain was pathogenic
6 Griffith Discovers Transformation 1. Mice injected with live cells of harmless strain R. 2. Mice injected with live cells of killer strain S. 3. Mice injected with heat-killed S cells. 4. Mice injected with live R cells plus heatkilled S cells. Mice live. No live R cells in their blood. Mice die. Live S cells in their blood. Mice live. No live S cells in their blood. Mice die. Live S cells in their blood. Figure 13.3 Page 218
7 Transformation What happened in the fourth experiment? The harmless R cells had been transformed by material from the dead S cells Descendents of the transformed cells were also pathogenic
8 Oswald & Avery What is the transforming material? Cell extracts treated with protein-digesting enzymes could still transform bacteria Cell extracts treated with DNA-digesting enzymes lost their transforming ability Concluded that DNA, not protein, transforms bacteria
9 Bacteriophages Viruses that infect bacteria Consist of protein and DNA Inject their hereditary material into bacteria bacterial cell wall cytoplasm plasma membrane Figure 13.4b Page 219
10 Hershey & Chase s Experiments Created labeled bacteriophages Radioactive sulfur Radioactive phosphorus Allowed labeled viruses to infect bacteria Asked: Where are the radioactive labels after infection?
11 virus particle labeled with 35 S virus particle labeled with 32 P Hershey and Chase bacterial cell (cutaway view) Results label outside cell label inside cell Figure 13.5 Page 219
12 Initial Question: Who established that DNA not a protein was responsible for the inheritance of genetic information? OBJECTIVES: Create a timeline of the history of DNA study. Describe the bonding between the nitrogenous bases in the DNA molecule. STANDARDS: 3.A.1 DNA and in some cases RNA is the primary source of heritable information B.1 Bio.B.1.2
13 Structure of Nucleotides in DNA Each nucleotide consists of Deoxyribose (5-carbon sugar) Phosphate group A nitrogen-containing base Four bases Adenine, Guanine, Thymine, Cytosine
14 Nucleotide Bases phosphate group ADENINE (A) GUANINE (G) deoxyribose THYMINE (T) CYTOSINE (C) Figure 13.6 Page 220
15 Composition of DNA Chargaff showed: Amount of adenine relative to guanine differs among species Amount of adenine always equals amount of thymine and amount of guanine always equals amount of cytosine A=T and G=C
16 Rosalind Franklin s Work Was an expert in X-ray crystallography Used this technique to examine DNA fibers Concluded that DNA was some sort of helix
17 Watson-Crick Model DNA consists of two nucleotide strands Strands run in opposite directions Strands are held together by hydrogen bonds between bases A binds with T and C with G Molecule is a double helix
18 Watson-Crick Model Figure 13.7 Page 221
19 DNA Structure Helps Explain How It Duplicates DNA is two nucleotide strands held together by hydrogen bonds Hydrogen bonds between two strands are easily broken Each single strand then serves as template for new strand
20 INITIAL QUESTION: Describe the basic structure of DNA. OBJECTIVES: Diagram the process of DNA replication STANDARDS: 3.A.1 DNA and in some cases RNA is the primary source of heritable information B.1 Bio.B.1.2
21 new Figure 13.9 Page 222 DNA Replication Each parent strand remains intact Every DNA molecule is half old and half new old old new
22 Base Pairing during Replication Each old strand serves as the template for complementary new strand Figure Page 223
23 Enzymes in Replication Enzymes unwind the two strands DNA polymerase attaches complementary nucleotides DNA ligase fills in gaps Enzymes wind two strands together
24 A Closer Look at Strand Assembly Energy for strand assembly is provided by removal of two phosphate groups from free nucleotides newly forming DNA strand one parent DNA strand Figure Page 223
25 Continuous and Discontinuous Assembly Strands can only be assembled in the 5 to 3 direction Figure Page 223
26 DNA Repair Mistakes can occur during replication DNA polymerase can read correct sequence from complementary strand and, together with DNA ligase, can repair mistakes in incorrect strand
27 TICKET OUT THE DOOR: Replicate the following strand of DNA: A-A-C-T-G-A-T-T-C-C-T-A-G
28 Cloning Making a genetically identical copy of an individual Researchers have been creating clones for decades These clones were created by embryo splitting
29 Dolly: Cloned from an Adult Cell Showed that differentiated cells could be used to create clones Sheep udder cell was combined with enucleated egg cell Dolly is genetically identical to the sheep that donated the udder cell
30 More Clones Mice Cows Pigs Goats Guar (endangered species)
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