DNA, RNA, and Protein. The Whole Story
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1 DNA, RNA, and Protein The Whole Story
2 They didn t always know DNA was the Genetic Material. But they did know that the genetic material needed to do four things.
3 The Master Molecule Contains Information Transmits information to the ribosomes Replicates Makes Mistakes (Mutations)
4 The unique characteristics of an organism are encoded in DNA
5 After scientists discovered that DNA was the Genetic Material, it then became the task to figure out what it looked like.
6 They knew that DNA was made up of nucleotides which are composed of
7 Sugar Nitrogen Base Phosphate
8 They also knew that there were four nitrogen bases
9 Adenine Thymine Guanine Cytosine
10 Chargaff s Rule Thymine - Adenine Cytosine - Guanine
11 Rosalind Franklin took X-ray photographs of DNA that Suggested...?
12 DNA was Helical
13 Watson and Crick Using these, and other experiment s s data, Watson and Crick put together the Model of DNA. They called the shape... A Double Helix
14 The Double Helix Two spiraled strands of nucleotides whose bases face each other
15 From here it was rather easy to figure out how DNA replicated itself
16 Using Chargaff s Rule, A Hydrogen Bonds with T and C Hydrogen Bonds with G and the model, they determined...
17 During DNA replication, the molecule uncoils and unzips down the middle. As the base pairs separate, complimentary nucleotides are added by DNA polymerase, and the molecules recoils.
18 If We Have a DNA strand of... A T C G A T C G G C T A
19 First they seperate... A T C G A T C G G C T A
20 First they seperate... A T C G A T C G G C T A
21 First they seperate... A C A C G T T G T G C A
22 Then new nucleotides are put in.. A T T C G A T C G G C T T A
23 Then new nucleotides are put in.. A T T C G G A T C G G G C T T A
24 Then new nucleotides are put in.. A T T C G G A T T C C G G G C T T A
25 Then new nucleotides are put in.. A T T C G G A T A T C G C G G G C T T A
26 Then new nucleotides are put in.. A T T C G C G A T A T C G C G G C G C T T A
27 Until you have two complete strands. A T A T C G C G A T A T C G C G G C G C T A T A
28 Each time DNA replicates we get two new strands that are exactly like the original. Unless,
29 there is a mispairing, such as A with C. Then DNA Repair (enzymes and polymerases) come along and fix the DNA. Recognize the mistake Remove what is wrong Replace with what is right
30 Some Mutations don t get fixed It could be Harmful -Cause the organism not to grow or to die -Cause cancers It could be Beneficial -Creates variation in a species and the variation could be good.
31 Now we know how to make more DNA. Is this all we need to know for life?
32 No, Our bodies use Protein for its Life Processes Since, the information of how the proteins are arranged (and thus, its function) is stored in DNA There must be some connection must exist between the two. Which is...
33 ribonucleic acid
34 DNA vs. RNA double- stranded contains deoxyribose contains thymine single- stranded contains ribose contains uracil
35 Two Steps 1. DNA to mrna Transcription 2. mrna to Protein Translation
36 DNA uncoils RNA polymerase reads DNA template and matches up RNA nucleotides RNA strand splits off and DNA rejoins Transcription DNA to mrna
37 Before it goes out of the nucleus, RNA Processing
38 Introns and Exons Introns get spliced out of chain Exons are left and get sent out of the nucleus
39 Transposons Genes that jump around on the chromosome Changes expression of trait
40 The Genetic Code How can only four nucleotides code for 20 different amino acids? The nucleotides in mrna are read in groups of three Called a codon
41 AAACACGGU becomes AAA-CAC CAC-GGU
42 Each codon codes for a specific amino acid
43 So, AAA-CAC CAC-GGU becomes Lysine-Histidine Histidine-Glycine
44 How, You Ask?
45 Translation RNA to Protein mrna exits nucleus and enters cytoplasm
46 Ribosomes attatch to mrna and attract a trna The trna contains an anticodon - three nucleotides that base-pair to a specific codon in mrna The trna is connected to an amino acid
47 The ribosome binds another trna, joins the two amino acids with a Peptide Bond, cuts the bond it had with its trna,and releases that trna to bind with another amino acid
48 The ribosome advances along the mrna, binding new trna molecules and amino acids until it reaches a stop codon.
49 The result is an amino acid chain, otherwise know as a Protein
50 Gene Expression is controlled by Promoters - promote RNA polymerase to bind Repressors - represses the making of genes by binding to the site that RNA polymerase binds to, thus the polymerase can t
51 Gene Expression is controlled by Inducers - bind to the repressor and take it away, so the RNA polymerase can bind to it an do its thing
52 And that is the Jist of it. Reading Resource - Chapter 12 pages
53 Rest of this Hour Internet Demo and Decoding Practice
54 Tomorrow
55 it/dna/shockwave.html
DNA. translation. base pairing rules for DNA Replication. thymine. cytosine. amino acids. The building blocks of proteins are?
2 strands, has the 5-carbon sugar deoxyribose, and has the nitrogen base Thymine. The actual process of assembling the proteins on the ribosome is called? DNA translation Adenine pairs with Thymine, Thymine
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