UltraClean PCR Clean-Up Kit Sample (Catalog No S)
|
|
- Maximillian Logan
- 5 years ago
- Views:
Transcription
1 UltraClean PCR Clean-Up Kit Sample (Catalog No S) Information for Ordering Product Catalog No. Quantity Preps Preps Preps Instruction Manual Please recycle Version:
2 Table of Contents Introduction... 3 Protocol Overview... 3 Specifications... 3 Flow Chart... 4 Equipment Required... 5 Kit Contents & Storage... 5 Precautions & Warnings... 5 Protocols: Experienced User Protocol... 6 Detailed Protocol (Describes what is happening at each step)... 7 Vacuum Manifold Protocol... 9 Hints & Troubleshooting Guide Contact Information Products recommended for you
3 Introduction The UltraClean PCR Clean-Up Kit is designed to purify PCR products directly from a PCR or enzyme reaction in just 3 minutes without running an agarose gel. If you sequence your PCR reactions or have applications where efficient removal of PCR primers is critical, this kit is your solution. All reagents are optimized to remove primers, dntps and reaction components while purifying PCR reaction products in the size range of 60 bp to 10 kb. Protocol Overview With the addition of binding buffer, a novel silica spin filter is used to selectively bind the PCR or reaction product. Unwanted reaction components are passed through the filter by centrifugation. The desired product is then washed and recovered from the spin filter in certified DNA-free Tris buffer. The resulting DNA can be used for any downstream application. Specifications DNA Size range: 60 bp - 10 kb Binding capacity of Spin Filter: 20 g Final volume of DNA: 50 l Recovery rates: % High Throughput Options MO BIO offers a vacuum based protocol for faster processing without centrifugation for the DNA binding and column washing steps for Spin Filters. The MO BIO PowerVac Manifold allows for processing of up to 20 spin filter preps at a time using the PowerVac Mini Spin Filter Adapters. For additional high throughput options MO BIO offers the UltraClean -htp 96 Well PCR Clean-Up Kit for processing up to 2 x 96 samples using a centrifuge capable of spinning two 96 Well Blocks stacked (13 cm x 8 cm x 5.5 cm) at 2500 x g. For 96 well homogenization of soil, MO BIO offers the 96 Well Plate Shaker and Plate Adapter Set (MO BIO Catalog# & 11990, respectively.) This kit is for research purposes only. Not for diagnostic use. Other Related Products Catalog No. Quantity UltraClean -htp 96 Well PCR Clean-Up Kit x 96 preps 12 x 96 preps UltraClean Agarose, Molecular Biology Grade g 100 g UltraClean Lab Cleaner ml bottle 500 ml bottle 1 L bottle PowerVac Manifold manifold PowerVac Mini System unit + 20 adapters PowerVac Mini Spin Filter Adapters adapters 20 adapters 3
4 4
5 Equipment Required Microcentrifuge (10,000 x g) Pipettor (volumes required 50 l l) Vortex-Genie 2 Vortex (MO BIO Catalog# V or V-220) Kit Contents Component Amount SpinBind 1.5 ml SpinClean Buffer 0.75 ml Elution Buffer 0.15 ml Spin Filters 2 (Units in 2 ml Tubes) 2 ml Collection Tubes 2 Kit Storage Kit reagents and components should be stored at room temperature (15-30 C). Precautions Please wear gloves when using this product. Avoid all skin contact with kit reagents. In case of contact, wash thoroughly with water. Do not ingest. See Material Safety Data Sheets for emergency procedures in case of accidental ingestion or contact. All MSDS information is available upon request ( ) or at Reagents labeled flammable should be kept away from open flames and sparks. WARNING: SpinClean Buffer contains ethanol. It is flammable. Do not use bleach to clean the inside of the PowerVac Manifold or to rinse the PowerVac Mini Spin Filter Adapters when attached to the manifold. IMPORTANT NOTE FOR USE: Shake to mix the SpinBind before use. 5
6 Experienced User Protocol Please wear gloves at all times 1. Shake to mix the SpinBind before use. Add 5 volumes of SpinBind to your PCR reaction. Example: add 500 l to a 100 l PCR reaction. 2. Mix well by pipetting. If an oil overlay was used, you will now have two layers. The top layer is oil. 3. Transfer PCR/SpinBind mixture to a Spin Filter unit, while avoiding the transfer of oil. 4. Centrifuge seconds at a minimum 10,000 x g (approximately 13,000 rpm) in a tabletop microcentrifuge. 5. Remove the Spin Filter basket and discard the liquid flow-through from the tube by decanting. 6. Replace the Spin Filter basket in the same tube. 7. Add 300 l SpinClean Buffer to the Spin Filter. 8. Centrifuge seconds at a minimum 10,000 x g. 9. Remove Spin Filter basket and discard liquid flow through by decanting then replace basket back into the same tube. 10. Centrifuge seconds at minimum 10,000 x g. 11. Transfer Spin Filter to a clean 2 ml Collection Tube (provided). 12. Add 50 l of Elution Buffer (10 mm Tris) solution provided or sterile water directly onto the center of the white Spin Filter membrane. The choice of using Tris or water at this point will not affect yield. DNA is more stable for storage in Tris. 13. Centrifuge seconds at a minimum 10,000 x g. 14. Discard Spin Filter basket from the inside of the Spin Filter unit. Purified DNA is now in the 2 ml Collection Tube. The DNA will be free of all reaction components such as primers or linkers, enzyme, salt, and dntp s. Store DNA at -20 C. DNA is now ready to use. Thank you for choosing the UltraClean PCR Clean-Up Kit Sample. 6
7 Detailed Protocol (Describes what is happening at each step) Please wear gloves at all times 1. Shake to mix the SpinBind before use. Add 5 volumes of SpinBind to the PCR reaction. Example: add 500 l to a 100 l PCR reaction. What s happening: SpinBind is a buffered salt solution. By mixing it with your PCR reaction product, you are creating a ph buffered high salt condition. ph is critical after the addition of SpinBind. ph above 8 results in low DNA recovery. ph below 5, results in primers co-purifying with your sample DNA. Optimal ph range is for high recovery and total primer removal. 2. Mix well by pipetting. If an oil overlay was used, you will now have two layers. The top layer is oil. What s happening: Mixing well creates a homogeneous salt concentration throughout the sample tube. 3. Transfer PCR/SpinBind mixture to a Spin Filter unit, while avoiding the transfer of oil. What s happening: Although the oil does not affect DNA binding, it can be very messy if you do not remove it at this step. If you used a wax overlay, try to avoid carrying over any during this step. 4. Centrifuge seconds at a minimum 10,000 x g (approximately 13,000 rpm) in a tabletop microcentrifuge. What s happening: DNA in the size range from 60 bp to 10 kb will bind to the white silica spin filter membrane at the bottom of the silica spin filter unit. The liquid that passes through the membrane will contain unwanted components of the PCR reaction such as: PCR primers, dntps, enzyme, and buffer constituents. The desired PCR product DNA will bind to silica under high salt conditions. 5. Remove the Spin Filter basket and discard the liquid flow-through from the tube by decanting. What s happening: This is flow through waste. This contains Guanidine HCl so be sure to check with your lab safety office for proper disposal and handling. 6. Replace the Spin Filter basket in the same tube. 7. Add 300 l SpinClean Buffer to the Spin Filter. What s happening: SpinClean is a wash solution. It will remove any traces of unwanted contaminants while allowing the desired PCR product DNA to stay bound to the silica spin filter membrane. SpinClean is an ethanol based wash solution. It contains less than 80% ethanol. This solution is flammable so use caution near flames. 8. Centrifuge seconds at a minimum 10,000 x g. What s happening: As the SpinClean passes through the spin filter membrane, it cleans the PCR product DNA. 9. Remove Spin Filter basket and discard liquid flow through by decanting then replace basket back into the same tube. 7
8 10. Centrifuge seconds at minimum 10,000 x g. What s happening: This step will remove any last traces of SpinClean. The ethanol in the SpinClean can have a negative affect on the DNA purity. 11. Transfer Spin Filter to a clean 2 ml Collection Tube (provided). 12. Add 50 l of Elution Buffer (10mM Tris) solution provided or sterile water directly onto the center of the white Spin Filter membrane. The choice of using Tris or water at this point will not affect yield. DNA is more stable for storage in Tris. What s happening: Placing the Elution Buffer in the center of the small white silica membrane will make sure the entire membrane is wetted. This will result in more efficient release of the desired DNA. 13. Centrifuge seconds at a minimum 10,000 x g. What s happening: As the Elution Buffer passes through the silica membrane, DNA is released, and it flows through the membrane, and into the collection tube. The DNA is released because it can only bind to the silica spin filter membrane in the presence of salt. Elution Buffer is 10mM Tris ph. 8 and does not contain salt. 14. Remove Spin Filter basket from the 2 ml Collection Tube. Seal tube and store DNA at -20 C. What s happening: Purified DNA is now in the collection tube. The DNA is UltraClean and free of all reaction components such as primers or linkers, enzyme, salt, and dntp s. The DNA is now ready to use for any application. The DNA is in a 50 l volume. To concentrate it, see the Hints and Troubleshooting Guide. Thank you for choosing the UltraClean PCR Clean-Up Kit Sample. 8
9 Vacuum Protocol using the PowerVac Manifold Please wear gloves at all times For each sample lysate, use one Spin Filter column. Keep the Spin Filter in the attached 2 ml Collection Tube and continue using the Collection Tube as a Spin Filter holder until needed for the Vacuum Manifold Protocol. Label each Collection Tube top and Spin Filter column to maintain sample identity. You will need to provide 100% ethanol for step 4 of this protocol 1. For each prep, attach one aluminum PowerVac Mini Spin Filter Adapter (MO BIO Catalog# or ) into the Luer-Lok fitting of one port in the manifold. Gently press a Spin Filter column into the PowerVac Mini Spin Filter Adapter until snugly in place. Ensure that all unused ports of the vacuum manifold are closed. Note: Aluminum PowerVac Mini Spin Filter Adapters are reusable. 2. Transfer up to 650 l of prepared sample lysate (from step 2) to the Spin Filter column. 3. Turn on the vacuum source and open the stopcock of the port. Hold the tube in place when opening the stopcock to keep the spin filter steady. Allow the lysate to pass through the Spin Filter column. After the lysate has passed through the column completely, if you have more sample, load again with the next 650 l until all of the lysate has been loaded onto the Spin Filter column. Close the oneway Luer-Lok stopcock of that port. Note: If Spin Filter columns are filtering slowly, close the ports to samples that have completed filtering to increase the pressure to the other columns. 4. Load 800 l of 100% ethanol into the Spin Filter so that it completely fills the column. Open the stopcock while holding the column steady. Allow the ethanol to pass through the column completely. Close the stopcock. 5. Add 300 l of SpinClean Buffer to each Spin Filter. Open the Luer-Lok stopcock and apply a vacuum until SpinClean Buffer has passed through the Spin Filter completely. Continue to pull a vacuum for another minute to dry the membrane. Close each port. 6. Turn off the vacuum source and open an unused port to vent the manifold. If all 20 ports are in use, break the vacuum at the source. Make certain that all vacuum pressure is released before performing the next step. It is important to turn off the vacuum at the source to prevent backflow into the columns. 7. Remove the Spin Filter column and place in the original labeled 2 ml Collection Tube. Place into the centrifuge and spin at 13,000 g for 1 minute to completely dry the membrane. 8. Transfer the Spin Filter column to a new 2 ml Collection Tube and add 50 l of Elution Buffer to the center of the white filter membrane. 9. Centrifuge at room temperature for 30 seconds at 10,000 x g. 9
10 10. Discard the Spin Filter column. The DNA in the tube is now ready for any downstream application. No further steps are required. We recommend storing DNA frozen (-20 to -80 C). Elution Buffer is 10 mm Tris and contains no EDTA. To concentrate the DNA see the Hints & Troubleshooting Guide. Thank you for choosing the UltraClean PCR Clean-Up Kit Sample. 10
11 Hints and Troubleshooting Guide Concentrating the DNA Your final volume will be 50 l. If this is too dilute for your purposes, add 2 l of 5 M NaCl and mix. Then add 100 l of 100% cold ethanol. Mix. Centrifuge at 10,000 x g for 5 minutes. Decant all liquid. Dry residual ethanol in a speed vac or desiccator or ambient air. Resuspend precipitated DNA in desired volume. DNA Floats Out of Well When Loaded on a Gel Residual SpinClean in the final sample. Prevent this by being careful in step 11 not to transfer liquid onto the bottom of the spin filter basket. Ethanol precipitate to remove residues of SpinClean. (See procedure for Concentrating the DNA above.) Low Recovery Low recoveries can be due to not mixing SpinBind well with your sample in step 2. Incomplete removal of SpinClean can also reduce yields. Make sure your centrifuge is spinning at 10,000 x g minimum. Enzyme Reactions Inhibited If you chose to elute in a buffer containing EDTA, you may see inhibition of subsequent enzymatic reactions. Re-purify the sample with this kit and use the Tris solution Elution Buffer provided for the elution step. Cleaning of the PowerVac Mini Spin Filter Adapters It is recommended to rinse the PowerVac Mini Spin Filter Adapters promptly after use to avoid salt build up. To clean the PowerVac Mini Spin Filter Adapters, rinse each adapter with DI water followed by 70% ethanol and flush into the manifold base. Alternatively, remove the adapters and wash in laboratory detergent and DI water. PowerVac Mini Spin Filter Adapters may be autoclaved. Do not use bleach to clean the PowerVac Mini Spin Filter Adapters while attached to the PowerVac Manifold. Bleach should never be mixed with solutions containing guanidine and should not be used to clean the PowerVac Manifold. For more information on cleaning the PowerVac Manifold, please refer to the PowerVac Manifold manual. 11
12 Contact Information Technical Support: Phone MO BIO Laboratories, Inc. Toll Free , or Fax: Mail: MO BIO Laboratories, Inc, 2746 Loker Ave West, Carlsbad, CA Ordering Information: Direct: Phone MO BIO Laboratories, Inc. Toll Free , or Fax: Mail: MO BIO Laboratories, Inc, 2746 Loker Ave West, Carlsbad, CA For the distributor nearest you, visit our web site at 12
13 Products recommended for you For a complete list of products available from MO BIO Laboratories, Inc., visit Description Catalog No. Quantity x 96 preps UltraClean -htp 96 Well PCR Clean-Up Kit x 96 preps UltraClean GelSpin DNA Extraction Kit preps 100 preps 250 preps UltraClean 15 DNA Purification Kit preps PowerClean DNA Clean-Up Kit preps Dye Dots plates plates 13
UltraClean Standard Mini Plasmid Prep Kit Sample (Catalog No S)
UltraClean Standard Mini Plasmid Prep Kit Sample (Catalog No. 12301-S) Information for Ordering Product Catalog No. Quantity 12301-50 50 Preps 12301-100 100 Preps 12301-250 250 Preps Instruction Manual
More informationUltraClean PCR Clean-Up Kit
UltraClean PCR Clean-Up Catalog No. Quantity 12500-50 50 Preps 12500-100 100 Preps 12500-250 250 Preps Instruction Manual New protocol instruction: Shake the SpinBind Solution before using to ensure consistent
More informationPowerSoil DNA Isolation Kit
PowerSoil DNA Isolation Kit Catalog No. Quantity 12888-50 50 Preps 12888-100 100 Preps Instruction Manual Introduction The PowerSoil DNA Isolation Kit* is comprised of a novel and proprietary method for
More informationPowerFood Microbial DNA Isolation Kit Sample (For isolation of genomic DNA from cultured food) (Catalog No S)
PowerFood Microbial DNA Isolation Kit Sample (For isolation of genomic DNA from cultured food) (Catalog No. 21000-S) Information for Ordering Product Catalog No. Quantity 21000-50 50 Preps 21000-100 100
More informationPowerWater DNA Isolation Kit (For isolation of genomic DNA from membrane filtered water samples)
PowerWater DNA Isolation Kit (For isolation of genomic DNA from membrane filtered water samples) Catalog No. Quantity Filters 14900-50-NF 50 Preps No filters 14900-100-NF 100 Preps No filters Instruction
More informationPowerMax Soil DNA Isolation Kit
PowerMax Soil DNA Isolation Kit Catalog No. Quantity 12988-10 10 Preps Instruction Manual Inhibitor Removal Technology (IRT) is a registered trademark of MO BIO Laboratories, Inc. and is covered by the
More informationUltraClean Mega Soil DNA Kit Catalog number: Preps (For up to 10 grams of soil)
UltraClean Mega Soil DNA Kit Catalog number: 12900-10 10 Preps (For up to 10 grams of soil) Instruction Manual Introduction Use this kit for isolating DNA from 5-10 g soil samples. The basic procedure
More informationRNA PowerSoil DNA Elution Accessory Kit Sample (Catalog No S)
RNA PowerSoil DNA Elution Accessory Kit Sample (Catalog No. 12867-S) Information for Ordering Product Catalog No. Quantity 12867-25 25 Preps Instruction Manual Please Recycle Version: 11172015 1 Table
More informationUltraClean Endotoxin-Free Mini Plasmid Prep Kit
UltraClean Endotoxin-Free Mini Plasmid Prep Kit Catalog No. Quantity 12311-100 100 Preps Instruction Manual Please recycle Version: 11172015 1 Table of Contents Introduction... 3 Protocol Overview... 3
More informationUltraClean Midi Plasmid Prep Kit
UltraClean Midi Plasmid Prep Kit Catalog No. Quantity 12700-20 20 Preps Instruction Manual Please recycle Version: 05232014 1 Table of Contents Introduction... 3 Protocol Overview... 3 Flow Chart... 4
More informationOn-Spin Column DNase I Kit (RNase-Free)
On-Spin Column DNase I (RNase-Free) Catalog No. Quantity 15100-50 Instruction Manual F Please recycle Version: 02022009 1 Introduction Use this product to remove contaminating genomic DNA from silica membrane
More informationBiOstic Bacteremia DNA Isolation Kit Sample Catalog No S (For use with cultured blood for the enrichment of bacteria)
BiOstic Bacteremia DNA Isolation Kit Sample Catalog No. 12240-S (For use with cultured blood for the enrichment of bacteria) Information for Ordering Product Catalog No. Quantity 12240-50 50 preps Instruction
More informationPowerMicrobiome RNA Isolation Kit Sample (Catalog No S)
PowerMicrobiome RNA Isolation Kit Sample (Catalog No. 26000-S) Information for Ordering Product Catalog No. Quantity 26000-50 50 Preps Instruction Manual Inhibitor Removal Technology (IRT) is a registered
More informationUltraClean 6 Minute Mini Plasmid Prep Kit
UltraClean 6 Minute Mini Plasmid Prep Kit Catalog No. Quantity 12300-50 50 Preps 12300-100 100 Preps 12300-250 250 Preps Instruction Manual Introduction Use the UltraClean 6 Minute Mini Plasmid Prep Kit
More informationBiOstic Paraffin Removal Reagent
BiOstic Paraffin Removal Reagent Catalog No. Quantity 12251-50 Instruction Manual F Please recycle Version: 01232009 1 Introduction: Use this product in place of xylene prior to the isolation of genomic
More informationPowerMag Soil DNA Isolation Kit (Optimized for KingFisher )
PowerMag Soil DNA Isolation Kit (Optimized for KingFisher ) Catalog No. Quantity Total Purifications 27000-4-KF 4 x 96 Preps (Flex) 384 or 32 x 12 Preps (Duo) Instruction Manual Please recycle Version:
More informationIntroduction. Kit components. 50 Preps GF-PC-050. Product Catalog No. 200 Preps GF-PC Preps GF-PC Preps SAMPLE
Introduction The GF-1 PCR Clean Up Kit is a system designed for rapid clean up of DNA bands ranging from 100bp to 20kb. The GF-1 PCR Clean Up Kit contains special buffers to provide the correct salt concentration
More informationPowerViral Environmental RNA/DNA Isolation Kit
PowerViral Environmental RNA/DNA Isolation Kit Catalog No. Quantity 28000-50 50 Preps + Bead Tubes Instruction Manual Please recycle Version: 03142016 1 Table of Contents Introduction... 3 Protocol Overview...
More informationPowerMag Microbiome RNA/DNA Isolation Kit (Optimized for KingFisher )
PowerMag Microbiome RNA/DNA Isolation Kit (Optimized for KingFisher ) Catalog No. Quantity Total Purifications 27600-4-KF 4 x 96 Preps (Flex) or 32 x 12 Preps (Duo) 384 Instruction Manual Please recycle
More informationDNase Max Kit. Catalog No Saving You Time For Life. Quantity: 50 preps. Please recycle
Saving You Time For Life DNase Max Kit Catalog No. 15200-50 Quantity: 50 preps Instruction Manual Version 1232014 Please recycle www.mobio.com T: 800.606-6246 T: 760.929-9911 technical@mobio.com 2 TABLE
More informationUltraClean BloodSpin DNA Isolation Kit
UltraClean BloodSpin DNA Isolation Catalog No. Quantity 12200-50 20 Preps 12200-250 250 Preps Instruction Manual Please recycle Version: 111315 1 Table of Contents Introduction... 3 Protocol Overview...
More informationBIO BASIC. PCR Purification Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only
BIO BASIC PCR Purification Kit 9K-006-0003s (10 preps) 9K-006-0003 (100 preps) 9K-006-0004 (200 preps) For Research Use Only Index PCR Purification Kit Introduction 3 Important Notes 4 General Protocol
More informationNoviPure Microbial Protein Kit (50)
Saving You Time For Life NoviPure Microbial Protein Kit (50) Catalog No. 47044 Quantity: 50 preps INSTRUCTION MANUAL Version 04202016 Please recycle www.mobio.com T: 800-606-6246 T: 760-929-9911 technical@mobio.com
More informationPowerMag Soil DNA Isolation Kit
Saving You Time For Life PowerMag Soil DNA Isolation Kit Catalog No. 27100-4-EP Quantity: 4 x 96 Preps (Flex) or 32 x 12 Preps (Duo) Total Purifications: 384 INSTRUCTION MANUAL Version 09182014 Please
More informationBiOstic FFPE Tissue RNA Isolation Kit (For use with formalin-fixed and paraffin-embedded tissues)
BiOstic FFPE Tissue RNA Isolation Kit (For use with formalin-fixed and paraffin-embedded tissues) Catalog No. Quantity 13250-50 50 preps Instruction Manual Please recycle Version: 02032014 1 Table of Contents
More informationAllPrep Bacterial DNA/RNA/Protein Sample (2)
Saving You Time For Life AllPrep Bacterial DNA/RNA/Protein Sample (2) Catalog No. 47050 Quantity: 2 preps INSTRUCTION MANUAL Version 09142016 Please recycle www.mobio.com T: 800-606-6246 T: 760-929-9911
More informationPowerLyzer UltraClean Microbial DNA Isolation Kit
PowerLyzer UltraClean Microbial DNA Isolation Catalog No. Quantity 12255-50 50 Preps Instruction Manual New protocol instruction: Shake Solution MD3 to mix before using to ensure consistent results. PowerLyzer
More informationUltraClean Soil DNA Isolation Kit
UltraClean Soil DNA Isolation Catalog No. Quantity 12800-50 50 Preps 12800-100 100 Preps Instruction Manual New protocol instruction: Shake Solution S3 to mix before using to ensure consistent results.
More informationUser Bulletin. Shipping & Storage. Gel/PCR DNA Isolation System. Protocol. WARNING: strong acids and oxidants (such as bleach) should not
User Bulletin VER.:17A Shipping & Storage Viogene Gel/PCR DNA Isolation System is shipped and storage at ambient temperature up to 12 months. Gel/PCR DNA Isolation System Purification of small-scale DNA
More informationPowerMag DNA Clean-Up Kit
Saving You Time For Life PowerMag DNA Clean-Up Kit (Optimized for KingFisher ) Catalog No. 27900-4-KF Quantity: 4 x 96 Preps (Flex) or 32 x 12 Preps (Duo) Total Purifications: 384 Instruction Manual Version
More informationProduct # Specifications. Kit Specifications Binding Capacity Per Well. 15 g Maximum Loading Volume Per Well 500 L
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com PCR Purification 96-Well Kit Product # 24800 Product Insert Norgen
More informationSequencing Reaction Clean-Up 96-Well Kit Product # 34400
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Sequencing Reaction Clean-Up 96-Well Kit Product # 34400 Product
More informationPowerMag Microbial DNA Isolation Kit
PowerMag Microbial DNA Isolation Kit Catalog No. Quantity Total Purifications 27200-4 4 x 96 Preps 384 Instruction Manual Please recycle Version: 06282016 1 Table of Contents Introduction... 3 Protocol
More informationUltraClean Standard Mini Plasmid Prep Kit
UltraClean Standard Mini Plasmid Prep Catalog No. Quantity 12301-50 50 Preps 12301-100 100 Preps 12301-250 250 Preps Instruction Manual New & Improved Please read for protocol changes! F Please recycle
More informationTBE Buffer, 10X. Product Data Sheet Catalog No. Quantity ml liter. Please recycle Version:
TBE Buffer, 10X (Tris-borate-EDTA) Product Data Sheet Catalog No. Quantity 15002-500 15002-1000 1 liter Please recycle Version: 01042012 1 Description TBE Buffer is used for polyacrylamide and agarose
More informationPowerPlant RNA Isolation Kit
PowerPlant RNA Isolation Kit Catalog No. Quantity 13500-50 50 Preps Instruction Manual Inhibitor Removal Technology (IRT) is a registered trademark of MO BIO Laboratories, Inc. and is covered by the following
More informationBIO BASIC. Gel Extraction Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only
BIO BASIC Gel Extraction Kit 9K-006-0001s (10 preps) 9K-006-0001 (100 preps) 9K-006-0002 (200 preps) For Research Use Only Index Gel Extraction Kit Introduction 3 Important Notes 4 General Protocol 5 Troubleshooting
More informationE.Z.N.A. Cycle Pure Kit
E.Z.N.A. Cycle Pure Kit D6492-00 5 preps V-spin D6492-01 50 preps V-spin D6492-02 200 preps V-spin D6493-00 5 preps Q-spin D6493-01 50 preps Q-spin D6493-02 200 preps Q-spin March 2017 E.Z.N.A. Cycle
More informationE.Z.N.A. Cycle Pure Kit
E.Z.N.A. Cycle Pure Kit D6492-00 5 preps V-spin D6492-01 50 preps V-spin D6492-02 200 preps V-spin D6493-00 5 preps Q-spin D6493-01 50 preps Q-spin D6493-02 200 preps Q-spin March 2017 E.Z.N.A. Cycle Pure
More informationPowerMax Soil DNA Isolation Kit
PowerMax Soil DNA Isolation Catalog No. Quantity 12988-10 10 Preps Instruction Manual New protocol instruction: Shake Solution C4 to mix before using to ensure consistent results. Inhibitor Removal Technology
More informationPowerWater DNA Isolation Kit (For isolation of genomic DNA from membrane filtered water samples)
PowerWater DNA Isolation (For isolation of genomic DNA from membrane filtered water samples) Catalog No. Quantity Filters 14900-50-NF 50 Preps No filters 14900-100-NF 100 Preps No filters Instruction Manual
More informationQuick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection
INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator Catalog No. D4080 Highlights Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double
More informationQuick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection
INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator Catalog No. D4080 Highlights Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double
More informationPowerWater RNA Isolation Kit (For isolation of total RNA from membrane filtered water samples)
PowerWater RNA Isolation Kit (For isolation of total RNA from membrane filtered water samples) Catalog No. Quantity Filters 14700-50-NF 50 Preps No filters Instruction Manual Inhibitor Removal Technology
More informationSequencing Reaction Clean-Up Kit Product # 34500
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Sequencing Reaction Clean-Up Kit Product # 34500 Product Insert
More informationPresto 96 Well PCR Cleanup Kit
Instruction Manual Ver. 02.10.17 For Research Use Only Presto 96 Well PCR Cleanup Kit 96DFH02 (2 x 96 well plates/kit) 96DFH04 (4 x 96 well plates/kit) 96DFH10 (10 x 96 well plates/kit) Advantages Sample:
More informationTaKaRa MiniBEST Plasmid Purification Kit Ver.4.0
Cat. # 9760 For Research Use TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 Product Manual Table of Contents I. Description... 3 II. Kit Components... 3 III. Shipping and Storage... 4 IV. Preparation
More informationAxyPrep PCR Clean-up Kit AxyPrep-96 PCR Clean-up Kit
AxyPrep PCR Clean-up Kit AxyPrep-96 PCR Clean-up Kit For the purification of amplicons from PCRs Kit contents, storage and stability AxyPrep PCR Clean-up Kit Cat. No. AP-PCR-4 AP-PCR-50 AP-PCR-250 Kit
More informationProduct # Specifications. Kit Specifications 50 g 650 L All sizes, including small RNA (<200 nt)
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Leukocyte RNA Purification Kit Product # 21200 Product Insert
More informationPowerClean DNA Clean-Up Kit
PowerClean DNA Clean-Up Catalog No. Quantity 12877-50 50 Preps Instruction Manual New protocol instruction: Shake Solution 5 to mix before using to ensure consistent results. Inhibitor Removal Technology
More informationFast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.
INSTRUCTION MANUAL ZymoPURE Plasmid Miniprep Kit Catalog Nos. D4209, D4210, D4211 & D4212 (Patent Pending) Highlights Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using
More informationVortex Adapters for Vortex-Genie 2
Vortex Adapters for Vortex-Genie 2 Catalog No. Holds 13000-V1 12 (1.5-2.0 ml) Tubes 13000-V1-24 24 (1.5-2.0 ml) Tubes 13000-V1-15 4 (15 ml or 5 ml) Tubes 13000-V1-50 2 (50 ml) Tubes 13000-V1-5 6 (5ml only)
More informationPowerLyzer PowerSoil DNA Isolation Kit
PowerLyzer PowerSoil DNA Isolation Catalog No. Quantity 12855-50 50 Preps Instruction Manual New protocol instruction: Shake Solution C4 to mix before using to ensure consistent results. PowerLyzer Products
More informationPowerLyzer PowerSoil DNA Isolation Kit
PowerLyzer PowerSoil DNA Isolation Catalog No. Quantity 12855-50 50 Preps 12855-100 100 Preps Instruction Manual New protocol instruction: Shake Solution C4 to mix before using to ensure consistent results.
More informationGenepHlow Gel Extraction Kit
Instruction Manual Ver. 02.10.17 For Research Use Only GenepHlow Gel Extraction Kit DFG004 (4 Preparation Sample Kit) DFG100 (100 Preparation Kit) DFG300 (300 Preparation Kit) Advantages Convenient: includes
More informationLeukocyte RNA Purification 96-Well Kit Product # 37800
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Leukocyte RNA Purification 96-Well Kit Product # 37800 Product
More informationProduct # Specifications. Kit Specifications Column Binding Capacity 50 µg Maximum Column Loading Volume 700 µl
344 Merritt Street St. Catharines, ON, Canada L2T 1K6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Leukocyte RNA Purification Kit Product # 21200 Product Insert
More informationGel/PCR Extraction Kit
Gel/PCR Extraction Kit Item No: EX-GP200 (200rxns) Content Content Binding Buffer BD Wash Buffer PE Elution Buffer (10 mm Tris-HCl, ph 8.5) Spin Columns EX-GP200 80 ml 20 mlx3 10 ml 200 each Description
More informationLarge DNA Fragments Extraction Kit
Instruction Manual Ver. 04.25.17 For Research Use Only Large DNA Fragments Extraction Kit DFL004 (4 Preparation Sample Kit) DFL100 (100 Preparation Kit) DFL300 (300 Preparation Kit) Advantages Efficient:
More informationPlasmid DNA Extraction Miniprep Kit
BIO BASIC Plasmid DNA Extraction Miniprep Kit 9K-006-0009s (10 preps) 9K-006-0009 (100 preps) 9K-006-0010 (200 preps) For Research Use Only Index Plasmid DNA Extraction Miniprep Kit Introduction 3 Important
More informationProduct # Specifications. Kit Specifications Column Binding Capacity. 10 g Maximum Weight of Gel Slice Average DNA Recovery 70 90%
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com DNA Gel Extraction Kit Product # 13100 Product Insert The Norgen
More informationRNA Clean-Up and Concentration Kit Product # 23600, 43200
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com RNA Clean-Up and Concentration Kit Product # 23600, 43200 Product
More informationStrataPrep PCR Purification Kit
StrataPrep PCR Purification Kit INSTRUCTION MANUAL Catalog #400771 and #400773 Revision A For In Vitro Use Only 400771-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this
More informationPCR and Sequencing Reaction Clean-Up 96-Well Kit (Magnetic Bead System) Product # 62700
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com PCR and Sequencing Reaction Clean-Up 96-Well Kit (Magnetic Bead
More informationFast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge spin-column.
INSTRUCTION MANUAL ZymoPURE II Plasmid Maxiprep Kit Catalog Nos. D4202 & D4203 (Patent Pending) Highlights Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge
More informationFast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge spin-column.
INSTRUCTION MANUAL ZymoPURE II Plasmid Maxiprep Kit Catalog Nos. D4202 & D4203 (Patent Pending) Highlights Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge
More informationPresto Food DNA Extraction Kit
Instruction Manual Ver. 06.28.17 For Research Use Only Presto Food DNA Extraction Kit Advantages FGD004 (4 Preparation Sample Kit) FGD100 (100 Preparation Kit) FGD300 (300 Preparation Kit) Sample: 200
More informationStool Nucleic Acid Isolation Kit Product 45600
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Stool Nucleic Acid Isolation Kit Product 45600 Product Insert
More informationBead Type (NaI) Gel Extraction Kits
Bead Type (NaI) Contents Kit Contents Principle Important Notes Bead Type (NaI) Gel Extraction Kit Protocol Troubleshooting Guide 2 3 3 4 5 Ordering Information 6 Kit Contents Catalog No. Number of preparations
More informationSelect-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085
INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085 Highlights Tunable: Quick 10 minute protocol for size selection can be tuned from 100 bp to 1000 bp with
More informationGene-Spin DNA Purification Kit - V3
Protech Technology 波仕特生物科技 Gene-Spin 1-4-3 DNA Purification Kit - V3 (For PCR/DNA clean-up & Gel Extraction) Innovative Tools for Nucleic Acid Purification This Kit is for research purposes only. Not for
More informationRNA PowerSoil DNA Elution Accessory Kit
RNA PowerSoil DNA Elution Accessory Catalog No. Quantity 12867-25 25 Preps Instruction Manual Please Recycle Version: 08112011 1 Table of Contents Introduction... 3 Protocol Overview... 3 Equipment Required...
More informationBacterial Genomic DNA Isolation 96-Well Kit Product # 17950
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Bacterial Genomic DNA Isolation 96-Well Kit Product # 17950 Product
More informationPresto 96 Well gdna Bacteria Kit
Presto 96 Well gdna Bacteria Kit 96GBB02 (2 x 96 well plates/kit) 96GBB04 (4 x 96 well plates/kit) 96GBB10 (10 x 96 well plates/kit) Instruction Manual Ver. 05.04.17 For Research Use Only Advantages Sample:
More informationProteinase K Solution Product Data Sheet
Proteinase K Solution Product Data Sheet Catalog No. Quantity 1222-2 2 ml This product is for research purposes only. Not for diagnostic use. Description Proteinase K Solution from Tritirachium Album (Endopeptidase
More informationUrine DNA Isolation Maxi Kit (Slurry Format) Product # 50100
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation Maxi Kit (Slurry Format) Product # 50100 Product
More informationSoil DNA Extraction Kit
Instruction Manual Ver. 09.23.16 For Research Use Only Soil DNA Extraction Kit Advantages IB47800 (4 Preparation Sample Kit) IB47801 (50 Preparation Kit) IB47802 (100 Preparation Kit) Sample: 250-500 mg
More informationPlus DNA Clean/Extraction Kit
Plus DNA Clean/Extraction Kit Cat. # : DP034P/ DP034P-300 Size : 50/300 Reactions Store at RT For research use only 1 Description: The Plus DNA Clean/Extraction Kit is designed to extract DNA fragments
More informationProduct # Specifications. Kit Specifications Maximum DNA Input. 200 g Maximum DNA Volume Input Final Endotoxin Levels
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Endotoxin Removal Kit (Midi) Product # 52200 Product Insert Norgen
More informationPresto Mini Plasmid Kit
Instruction Manual Ver. 03.06.17 For Research Use Only Presto Mini Plasmid Kit PDH004 (4 Preparation Sample Kit) PDH100 (100 Preparation Kit) PDH300 (300 Preparation Kit) Advantages Sample: 1-7 ml of cultured
More informationPowerLyzer UltraClean Tissue & Cells RNA Isolation Kit
PowerLyzer UltraClean Tissue & Cells RNA Isolation Kit Catalog No. Quantity 15055-50 50 Preps Instruction Manual PowerLyzer Products PowerLyzer DNA and RNA Isolation kits combine either, glass, ceramic
More informationISOLATE II Blood DNA Kit. Product Manual
ISOLATE II Blood DNA Kit Product Manual 2 Product Manual www.bioline.com/isolate Blood DNA Kit ISOLATE II Blood DNA Kit ISOLATE II Blood DNA Kit 1 Kit contents 04 2 Description 04 3 Storage 05 4 Safety
More informationPlant DNA Isolation Kit (Magnetic Bead System) 50 Preps Product # 58200
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plant DNA Isolation Kit (Magnetic Bead System) 50 Preps Product
More informationSize-Select Kit for NGS Library Preparation Product # 53600
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Size-Select Kit for NGS Library Preparation Product # 53600 Product
More informationKit Specifications 45 g. 45 g of RNA 8 L
RNA Clean-Up and Concentration Micro-Elute Kit Product # 61000 Product Insert Norgen s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration
More informationE.Z.N.A. Gel Extraction Kit
E.Z.N.A. Gel Extraction Kit D2500-00 5 preps D2500-01 50 preps D2500-02 200 preps D2501-00 5 preps D2501-01 50 preps D2501-02 200 preps Manual Date: November 2018 Revision Number: v3.1 E.Z.N.A. Gel Extraction
More informationPlasma/Serum RNA/DNA Purification Mini Kit Product# 55200
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plasma/Serum RNA/DNA Purification Mini Kit Product# 55200 Product
More informationLysis Buffer A Wash Solution A Elution Buffer B Mini Filter Spin Columns 50 Collection Tubes 50 Elution tubes (1.7 ml) 100 Product Insert 1
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation Kit (Slurry Format) Product # 48800 Product
More informationPlasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions
Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions Maxim Biotech, Inc. 780 Dubuque Avenue, So. San Francisco, CA 94080, U.S.A. Tel: (800) 989-6296
More informationMicro-Elute DNA Clean/Extraction Kit
Micro-Elute DNA Clean/Extraction Kit (PCR Clean-up/Gel Extraction/DNA Clean-Up /Concentration Kit) Cat. # : DP034ME/ DP034ME-300 Size : 50/300 Reactions Store at RT For research use only 1 Description:
More informationE.Z.N.A. FFPE RNA Kit. R preps R preps R preps
E.Z.N.A. FFPE RNA Kit R6954-00 5 preps R6954-01 50 preps R6954-02 200 preps January 2015 E.Z.N.A. FFPE RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
More informationThe fastest, easiest, most reliable method for purification of up to 1.2 mg of ultra-pure endotoxin-free plasmid DNA.
INSTRUCTION MANUAL ZymoPURE - Express Plasmid Midiprep Kit Catalog No. D4213 (Patent Pending) Highlights Innovative ZymoPURE - Express pellet-free procedure bypasses the standard cell-pelleting and resuspension
More informationKit Specifications Number of Preps 25 Maximum Saliva Input Average Yield from 0.25 ml of Saliva 7 µg Average purity (OD260/280)
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Saliva DNA Isolation Kit Product #21410 Product Insert Norgen
More informationBlood DNA Isolation 96-Well Kit (Magnetic Bead System) Product # 62600
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Blood DNA Isolation 96-Well Kit (Magnetic Bead System) Product
More informationPresto 96 Well DNA Bacteria Advanced Kit
Instruction Manual Ver. 07.04.17 For Research Use Only Presto 96 Well DNA Bacteria Advanced Kit 96GBBA02 (2 x 96 well plates/kit) 96GBBA04 (4 x 96 well plates/kit) 96GBBA10 (10 x 96 well plates/kit) Advantages
More informationAverage Yields* Yeast DNA Yeast RNA Time to Complete 10 Purifications * Yield will vary depending on the type of sample processed
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Fungi/Yeast RNA/DNA Purification Kit Product # 35800 Product Insert
More informationKit Specifications 650 L 100 L. Product # (50 preps)
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Water RNA/DNA Purification Kit Product # 26480 Product Insert
More informationE.Z.N.A. M13 DNA Mini Kit
E.Z.N.A. M13 DNA Mini Kit D6900-00 5 preps D6900-01 50 preps EZ-96 M13 DNA Kit D1900-00 2 preps D1900-01 6 preps June 2013 E.Z.N.A. M13 DNA Mini Kit E-Z 96 M13 DNA Kit Table of Contents Introduction and
More informationPlasma/Serum Circulating Nucleic Acid Purification Mini Kit (Slurry Format)
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plasma/Serum Circulating Nucleic Acid Purification Mini Kit (Slurry
More information