CLINICAL MICROBIOLOGY HOSPITAL LABORATORY PRACTICE

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1 Jordan University of Science and Technology Faculty of Applied Medical Sciences Department of Medical Laboratory Sciences CLINICAL MICROBIOLOGY HOSPITAL LABORATORY PRACTICE Course Syllabus Course Title Course Code LM 476 Prerequisites Course Website Course Coordinator Course Information CLINICAL MICROBIOLOGY HOSPITAL LABORATORY PRACTICE Al-Shboul Office Location M5 L-5, Rm 20 Office Phone # Ex Office Hours Lab Supervisor Monday and Wednesday: 2:30-3:30 p.m, or by appointment NA Course Description The main goal of this course is to train students of Medical Laboratory Sciences in the field of Clinical Microbiology. This will provide students with advanced skills in processing of clinical specimens for microbiological analysis, including isolation, characterization, identification, typing of medically important bacteria, virus, fungi and parasites, and performance of antimicrobial susceptibility testing. Title Author(s) Publisher Year Edition Textbook Any Clinical Laboratory Manual and Clinical Microbiology textbook will do Book Website Other references Any clinical laboratory text book Laboratory manuals The internet!

2 Assessment Assessment Expected Due Date Percentage Works Last week of classes 60% Final exam Last week of classes 40% Attendance Absolute for all students Course Objectives At the end of the course the students should be able to: Learn how to use the following equipment: Anaerobic Jar Biological class II hazard cabinet Freezer Incubator Properly utilize microscopes in diagnosis of microbial pathogens; this includes: To learn the basic components of the standard light microscope and the use of and routine daily maintenance of the standard light microscope. Microscopic examination of bacterial smears Properly utilize sterilization; this includes: Learn the principles of sterilization utilizing the following mechanisms: Moist heat (autoclave), Dry heat (hot air oven), and Filtration (cellulose membrane filter) Learn about: The materials utilized in a clinical microbiology laboratory and how to properly sterilize equipment and tools, and how to dispose of clinical microbiology waste products. Learn about Bacterial Anatomy and Physiology; this includes The basic components of bacteria and functions of these components. Describe the morphology and arrangements of bacteria i.e. rods, cocci, etc. Learn the definitions of the following: Aerobe Anaerobe Microaerophile Capnophile Prepare smears and perform various staining techniques of different clinical specimens and from liquid or solid media; the techniques include: Gram staining Acid fast staining Flagellar staining Capsular staining Spore staining Media preparation, sterilization and dispensing Define the following and their use Enrichment media Selective media Differential media Percentage

3 Identification Tests Knowledge about the different Identification Tests Including their principle, key media, reagents, technique and controls Packaged kit identification systems to identify Enterobacteriaceae API 20E RapID one system (Remel) The use and the principle of Enzyme Immunoassay (EIA) for the detection of antigens. Testing of susceptibility to antimicrobial agents (AST): Learn how to process different types of clinical specimens including: collection, specimen receipt and preliminary observations, transportation and storage of specimens, and processing specimens aerobically and anaerobically. Teaching & Learning Methods Course will include: Discussions Laboratory applications Self reading Laboratory manuals Teaching duration: 16 weeks Hospital Laboratory: 2 sessions per week; 3 hours each Reference(s) Useful Resources Internet-links to Diagnostic and Clinical Microbiology Jordan University of Science and Technology Library

4 Course Content Week Title of the Lecture Lecturer 1 Specimen collection, transport Specimen receipt and preliminary observations 2 Processing specimens (aerobic and anaerobic) 3 Media preparation, sterilization and dispensing 4 Smear preparation and staining (different types of stains) of different clinical specimens from liquid or solid media; Microscopic examination Inoculation of clinical samples into proper media and incubation under appropriate conditions Interpretations of cultures; Procedures for preliminary identification of bacterial isolates Testing of susceptibility to antimicrobial agents (AST): Agar dilution susceptibility test and Disk diffusion susceptibility test (principle, procedure, interpretation), Epsilometer test Reporting results Dispensing of infected substances, specimens and contaminated materials Packaged kit identification systems: API 20E RapID one system (Remel) Semi automated and automated identification systems Vitek system BACTEC system KIA, TSI for Carbohydrate fermentation Selective media Enrichment media Indole production MRVP tests Citrate utilization Urease production Decarboxylation of lysine Ornithine, and Arginine And others

5 14 Nitrate reduction Pigment production Esculin hydrolysis Obtaining (if applicable) and processing microbiology clinical samples, including: Body fluids Blood Urine 15 Stool Sputum CSF Throat swab Rectal swabs Vaginal swabs And others 16 Final Exam Week Additional Notes Attendance policy: For practical training is 90%. Hospital Lab attendance is mandatory. Students who fail to meet the course attendance requirements will be administratively withdrawn from class per the JUST Attendance Policy. Grades: Grades are recorded to one decimal place. The semester grade percentage will be determined by rounding up numbers 0.5 to the next whole number (e.g., 78.5% = 79% or 78.4% = 78%).

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