Bio Base Europe Pilot Plant Turning Grams into Tonnes

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1 Bio Base Europe Pilot Plant Turning Grams into Tonnes BioenNW International Conference Brussels, 24 September 2015 Muriel Dewilde Business Development Manager

2 Bio Base Europe Pilot Plant Mission statement To enable the transition to a sustainable bio-based economy through Development, scale-up and custom manufacturing of bio-based product and processes

3 Bio Base Europe Pilot Plant Key Facts Founded in 2008 Sister Company: Bio Base Europe Training Center Funded with two EU Interreg grants : 20 M Fully independent, no industrial shareholders Operational since employees

4 Located in the Port of Ghent

5 Ghent Bio-Economy Valley EuroSilo

6 Bridging the Gap Technological risk Capital requirements Gap in the innovation chain Fundamental research Research Laboratory scale Development /Optimization Demonstration Pilot scale Market Entry Commercialization Market-ready product Time

7 One Stop Shop: From Biomass to Refined Product Under One Roof Pretreatment Biocatalysis Fermentation Green Chemistry Downstream Purification

8 Examples Corn stover Paper pulp Oils and fats usks, bran, straw Agro Industrial by-products Bagasse, beet pulp, seed press cakes Algae Non-food crops Specialty carbohydrates Industrial Enzymes Fine Chemicals Solvents Polymers Flavors and fragrances Protein concentrates Surfactants Nutraceuticals Fuels

9 Business Model Open Innovation Projects Independence Flexibility Confidentiality Service Projects Publicly Funded Projects Bilateral Intellectual Property remains with customer Confidential 40 companies in 2014 Consortium BBI-JU, orizon 2020, FP7, ongoing projects Global Network along the Innovation Chain

10 The facility Pilot all 2: Fermentation and DSP Laboratory Maintenance Pilot all 3: Green chemistry (ATEX) Pilot all 1: Pretreatment, Biocatalysis, DSP

11 Process all 1 Biomass pretreatment, bio-catalysis and Downstream Processing

12 Process all 2 Fermentation and Purification

13 Process all 3 Green chemistry and Solvent Based Processes (ATEX)

14 Laboratory Analysis and Liter Scale Process Development

15 Biomass Pretreatment Steam Injection Organosolv Acid/Alkaline treatment Pressure reactions Enzymatic hydrolysis Solid/Liquid separation Multi tonne scale

16 Biocatalysis Aqueous, Two-phase and Three-phase Systems Batch, Fed-batch, Continuous igh solids contents Reactors up to 50 m³ Immobilization Whole cell biocatalysts Enzyme production

17 Fermentation Batch, Fed-batch, Chemostat Bacteria, Yeast, Fungi Aerobic, Anaerobic, Gas fermentation 7 L, 50 L, 150 L, 4.5 m³ Newly installed: 1.5 m³ and 15 m³

18 Green Chemistry Glass lined Reactors up to 5 m³ Filter Dryer, Conical Dryer Liq-Liq extraction column, Sol-Liq extraction Crystallisation, filtration For Chemical reactions Solvent based DSP Corrosive conditions

19 Downstream Purification igh speed separators Membrane filtration Ion exchange, activated carbon treatment ATEX solvent extraction, precipitation

20 Downstream Purification Evaporation Crystallisation Drying

21 Consortium Projects Development, Scale-up, Demonstration, Building Value Chains BioKatalyse Biocatalysis for chemical industry BioCluster Building new value chains Ghent SynGas Cluster Syngas to chemicals DemoProBio Butanol in situ product recovery (ISPR) IB2Market Biosurfactants, Specialty Carbohydrates Nano3Bio Chitosan ChitoBioEngineering Chito-Oligomers BioSurfing Soforolipids O O O O O Bio Base NWE Novosides Stimulating innovative SMEs Novel glucosides O O O N O O O O O N 3 C O O O O N 3 C O O O N 3 C O Visions 2G technologies for organic waste 3 C

22 Thank you Muriel Dewilde

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