Version No.: 2.0 ( )

Size: px
Start display at page:

Download "Version No.: 2.0 ( )"

Transcription

1

2 Kit for the extraction of genomic DNA from blood and tissue using MagListo Version No.: 2.0 ( ) Please read all the information in booklet before using the unit Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: MagListo is a trademark of Bioneer Corporation. Copyright Bioneer Corporation. All Rights Reserved.

3 Contents I. Overview II. Kit components III. Storage IV. Intended use V. Safety warnings and precautions VI. Warranty and liability VII. Technical assistance VIII. Quality management IX. Kit specifications Extraction of genomic DNA small amount of sample Recommended amounts of starting sample X. Sample preparation XI. Principle XII. Magnetic Nano Bead Information 6 XIII. XIV. XV. XVI. Guidelines for MagListo Magnetic Separation Rack Materials and Equipment Needed But Not Provided Types of the Magnetic Separation Rack Procedure Protocols Before You Begin 10 A. DNA Extraction from Whole Blood 10 B. DNA Extraction from Cultured Cell 15

4 C. DNA Extraction from Animal Tissue 20 D. DNA Extraction from Bacterial cells (Gram-Negative Bacteria) 25 E. DNA Extraction from Bacterial cells (Gram-Positive Bacteria) 26 F. DNA Clean-Up (DNA Purification) 27 XVII. Appendix Troubleshooting guide Experimental data 32 XVIII. Ordering information XIX. Explanation symbols 35 36

5 I. Overview Description MagListo 5M Genomic DNA Extraction Kit utilizes Magnetic Nano Beads to extract total DNA from various of sources, such as whole blood, animal tissue and cultured cells, with the aid of the MagListo Magnetic Separation Rack. The use of MagListo Magnetic Separation Rack along with the kit greatly increases user convenience by shortening the extraction time without centrifugation. Features and Benefits - Magnetic Nano Beads enable the rapid nucleic acid extraction - No requirement of expensive instruments - A single kit serves mini or midi scale experiment Applications Gene Cloning, PCR, Real-Time PCR, Southern Blotting, SNP genotyping II. Kit Components MagListo 5M Genomic DNA Extraction Kit *K-3603 Buffer 1 (Lysis) Buffer 2 (Binding) Buffer 3 (1 st Washing) Buffer 4 (2 nd Washing) Buffer 5 (3 rd Washing) Buffer 6 (Elution) Magnetic Nano Bead - DNA Proteinase K powder, lyophilized RNase A powder, lyophilized 25 ml x 1 ea 25 ml x 1 ea 120 ml x 1 ea 80 ml x 1 ea 120ml x 1 ea 25 ml x 1 ea 1.8 ml x 6 ea 25 mg x 2 ea 24 mg x 2 ea *Mini 100 rxn, Midi 15 rxn, Maxi 8 rxn 1

6 III. Storage MagListo 5M Genomic DNA Extraction Kit should be stored dry at room temperature. It can be stored for up to 2 years if it remains sealed. IV. Intended Use MagListo 5M Genomic DNA Extraction Kit is intended for research use only. This kit is not intended for human or veterinary diagnostics. V. Safety Warnings and Precautions Please inquire BIONEER s Customer Service Center to obtain a copy of the Material Safety Data Sheet (MSDS) for this product. Before, during and after using this kit, all potentially hazardous materials (i.e. materials that may have come in contacted with genetically recombinant samples) including tubes, tips and other kit contents should be processed and discarded in accordance with applicable and appropriate regulations of the municipality/government in which this product is being used. A user must also be equipped with basic experimental techniques required for correct execution of the extraction experiments described in this User s Guide. Some inventions applied in this kit may infringe existing patents in certain countries. The purchase of this kit does not include or provide a license to perform such patented inventions. Users may be required to obtain a license depending on the patent law of country where this product is being used. We do not condone nor recommend the unlicensed use of patented inventions. VI. Warranty and Liability All BIONEER products are manufactured and tested under strict quality control protocols. BIONEER guarantees the quality of all directly manufactured products during the warranty period of one (1) year from the date of purchase. If you find any issues regarding the product quality, please immediately contact BIONEER s Customer Service Center (sales@bioneer.com). BIONEER does not assume liability for misuse of the product, i.e. using the product for any purposes 2

7 other than its intended purpose as described in the User s Guide. BIONEER will only assume liability under the condition when the users disclose all related information regarding the issue to BIONEER in written form within 30 days after occurrence of the issue. VII. Technical Assistance At Bioneer, we pride ourselves on being responsive to your needs. Our Technical Service Departments are staffed by experienced scientists with extensive practical and theoretical expertise in Molecular Biology and the use of Bioneer products. If you have any questions or would like to find out more information about MagListo products, please contact us. We look forward to hearing from you! Technical Support For all technical questions and troubleshooting on Bioneer products and applications. Tel: sales@bioneer.co.kr In North America Tel: support@bioneer.us.com VIII. Quality Management Every aspect of our quality management system from product development to supplier qualification ensures that our products meet the world-class standards. Each lot of MagListo 5M Genomic DNA Extraction Kit is carefully tested by the quality control team. 3

8 IX. Kit Specifications Sample Type Mini (Typical yield) Midi (Typical yield) Maxi (Typical yield) Whole blood 200 μl ( < 10 μg) 2 ml ( < 80 μg) 4 ml ( < 150 μg) Cultured cell ~ 1 x 10 6 ( < 12 μg) ~ 5 x 10 6 (< 60 μg) ~ 1 x 10 7 (< 120 μg) Animal tissue ~ 25 mg ( < 10 μg) ~ 100 mg ( < 40 μg) ~ 250 mg (< 120 μg) Bacteria (Gram (-), (+)) ~ 1 x 10 9 (< 15 μg) ~ 5 x 10 9 (< 80 μg) ~ 1 x (< 150 μg) Expected purity A 260 / 280 > 1.8 * The DNA yield from samples with a low number of cells may be less than the figures shown in the table. ** For cultured cells, samples with cell number of ~10 4 can be used for micro scale extraction. Extraction of genomic DNA from small amount of sample MagListo 5M Genomic DNA Extraction Kit is able to extract genomic DNA from sample of low quantity. If sample contains low number of cells (< 1x10 4 ) or has small amount of DNA, it is recommended to add about 4 μg of carrier RNA to the starting sample. Ensure that the carrier DNA does not interfere with your downstream application. Carrier RNA can be removed later by RNase digestion. Please see DNA Extraction from Cultured Cell for Micro in page 15 for more details about DNA extraction from samples with a low number of cells. Recommended amounts of starting sample It is recommended to use the amounts in Table 1 as starting sample amount. Table1. Growth area and Average cell yield in various culture dishes. Cell culture dishes Growth area (cm 2 ) Average cell yield Multi well plate 6 well x well 4 4 x well 2 2 x well 1 1 x well x 10 4 Dishes 4

9 35 mm x mm 21 3 x mm 55 8 x mm x 10 7 Flasks 50 ml x ml 75 1 x 10 7 X. Sample preparation Several factors, such as harvesting method and storage of starting samples can influence the yield and purity of DNA. All specimens must be stored in a freezer or used immediately after collection. It is recommended to process the sample as soon as possible on ice, and to avoid repeated freezing and thawing. Blood Blood sample should immediately be used or stored in a collection tube containing the anticoagulants for blood (EDTA or ACDs). Samples can be stored for few days at 4 and at up to 1 year at -70. It is recommended rapidly thaw frozen blood sample in water bath (37 ) and to keep the blood sample on ice until use. Cultured cells Cultured cells can easily be harvested using a centrifuge. However, it might be difficult to extract genomic DNA if cultured cells are too clustered. In this case, trypsin can be used to detach each cell from the cluster. For extraction using MagListo 5M Genomic DNA Extraction Kit, number of cells should be less than 1 x 10 7, which is calculated with a cell counter. It is recommended to keep samples on ice until use. Tissue Tissue samples should immediately be used or stored at -70 upon harvest for optimal results. To disrupt tissue sample, grind it in a pestle and a mortar with liquid nitrogen. Alternatively, a homogenizer or a bead-beater can be used. Bacterial cells Bacterial cells can be processed in a shaking incubator for hours at 37. Optimal results can be obtained when harvested bacterial cells are immediately used or stored at between -20 and -80. Additional bacteriolytic agents like lysozome or lysostaphin should be used to break the multilayered cell wall of Gram-positive bacteria. For gram negative bacteria, these agents are not needed. 5

10 XI. Principle MagListo 5M Genomic DNA Extraction Kit is designed for the extraction of genomic DNA including high molecular weight DNA (up to 40 kb). The overall principle is based on the adsorption of DNA onto the Magnetic Nano Bead by chaotropic salt. For example, chaotropic agents in Buffer 2 (Binding) contains guanidine hydrochloride, as which removes water molecules around DNA and silica coated magnetic beads surface resulting in genomic DNA then being captured by magnetic beads. The Magnetic Nano Bead and nucleic acid complexes are pulled and fixed on the tube wall using a magnetic force, followed by washing with ethanol to remove debris and excessive salts. Finally, the captured nucleic acids are then eluted by Buffer 6 (Elution), an aqueous solution with an optimal ph. Sample Lysis Binding Washing Elution XII. Magnetic Nano Bead Information Description Magnetic Nano Beads have been developed to overcome shortcomings of existing resin and to automate purification process. The principle of extraction using the Magnetic Nano Beads is that coated functional group on the surface of the Magnetic Nano Beads bind DNA and With the Magnetic Nano Beads are then isolated using external magnetic field. Features Fast binding guarantees higher throughput automation Large surface area enables better sensitive assay Globular structure increases specificity by decreasing non-specific binding 6

11 Specification Matrix Silica-coated Fe 3 O 4 AccuNanoBead Silica Magnetic Nano Beads Average size Ligand 400nm -OH Working Temp Storage Store at room temperature upon receipt XIII. Guidelines for MagListo TM Magnetic Separation Rack Description MagListo TM Magnetic Separation Rack is designed for a fast, easy separation of the Magnetic NanoBeads. Bioneer offers various racks of different sizes- MagListo -8Ch for 8-tube strip and multipipette, MagListo -2 for 2 ml tube, MagListo -15 for 15 ml tube, MagListo -50 for 50 ml tube and MagListo -96 for 96 well microplate. These racks of different sizes allow users to choose the product according to their needs. The following are recommended when handling the MagListo Magnetic Separation Rack The product is made of acryl and plastic. Be careful not to drop the product as the dropping may break the product. When moving the product, take extra care not to drop the product as it may cause injury. If the product is breaks, do not discard it with bare hands as the sharp edges may cause injury. When an extracted or purified nucleic acid is spilled on the product, immediately rinse it with running water and clean it with 70% ethanol. Acetone, Toluene, or organic solvent may damage the acrylic and plastic part of the product, which may lead to malfunction of the product. Rinse the product immediately when spillage of any above mentioned solvents occurs as the expected DNA yield may not be obtained if the product is damaged. Make sure that do not spill a corrosive liquid on the magnet plate part of the product. If spillage occurs, immediately rinse it off with running water as it may corrode the magnet during storage and may degrade its performance. 7

12 XIV. Materials and Equipment Needed But Not Provided ml or 2 ml tube, 15 ml tube, 50 ml tube 2. Vortex mixer 3. Absolute ethanol 4. Thermal block or dry oven 5. Phosphate buffered saline (PBS) 6. MagListo Magnetic Separation Rack Types of the Magnetic Separation Rack Tube 1 ml tube with 8-cap strip 1.5 ml or 2 ml microcentrifuge tube 15 ml tube 50 ml tube MagListo Magnetic Separation Rack MagListo -8Ch Magnetic Separation Rack (Cat. no. TM-1000) MagListo -2 Magnetic Separation Rack (Cat. no. TM-1010) MagListo -15 Magnetic Separation Rack (Cat. no. TM-1020) MagListo -50 Magnetic Separation Rack (Cat. no. TM-1030) (Note) Please refer to the ordering information in this User s Guide for more information regarding catalog number of racks designed for specific size of tubes. 8

13 XV. Procedure-Genomic DNA Extraction 9

14 XVI. Protocols Before you begin 1. Completely dissolve Proteinase K (KB-0111) in 1,250 ul of nuclease-free water. Dissolved Proteinase K should be stored at Completely dissolve RNase A (KB-3101) in 600 ul of nuclease-free water. Dissolved RNase A should be stored at Buffer 2 (Binding) and Buffer 3 (1 st Washing) contain chaotropic salt. You should take appropriate laboratory safety precautions and wear gloves when handling this buffer. 4. If there is any precipitate in Buffer 1 (Lysis), incubate on a heating block at 56. A. DNA Extraction from Whole Blood for Mini /Midi /Maxi scale 1. Add 20 μl (mini) / 100 μl (midi) / 200 μl (maxi) of Proteinase K (see Before you begin ) to each tube. a. (Mini) Add Proteinase K to a 1.5 ml or 2 ml tube. b. (Midi) Add Proteinase K to a 15 ml tube. c. (Maxi) Add Proteinase K to a 50 ml tube. 2. Transfer 200 μl (mini) / 2 ml (midi) / 4 ml (maxi) of whole blood and buffy coat to the tubes containing proteinase K. (Note) If the sample volume is less than the indicated volume above, bring the sample volume to a total volume of 200 μl (mini) / 2 ml (midi) / 4 ml (maxi) by adding 1X PBS to achieve maximum lysis efficiency and yield. 3. (Lysis: 3-4) Add 200 μl (mini) / 2 ml (midi) / 4 ml (maxi) of Buffer 2 (Binding) to each tube and mix thoroughly using a vortex mixer. Make sure that you completely resuspend the sample to achieve maximum lysis efficiency. 4. Incubate the tubes at 60 for 10 min. 10

15 5. (DNA Precipitation) Add 400 μl (mini) / 4 ml (midi) / 8 ml (maxi) of absolute ethanol to each tube and mix well using a vortex mixer or by pipetting. 6. (DNA binding with Magnetic Nano Bead: 6-8) Add 100 μl (mini) / 500 μl (midi) / 1 ml (maxi) of Magnetic Nano Bead solution to the each tube and mix thoroughly using a vortex mixer until the beads are fully resuspended. (Note) Magnetic Nano Bead solution contains Magnetic Nano Beads. Please shake well or mix with a vortex mixer before use. 7. Place the tube on the MagListo -2 (mini) / MagListo -15 (midi) / MagListo -50 (maxi) Magnetic Separation Rack with the magnet plate attached and invert the rack gently 3 to 4 times until the beads bind tightly to the magnet. - Attachment of the magnet plate Combine the magnet plate to the stand. 8. Without removing the tubes from the MagListo Magnetic Separation Rack, discard the supernatant carefully and completely remove the remaining supernatant on a paper towel by blotting action. During this process, the magnetic crude pellet remains attached to the side of tube. - How to discard the supernatant Discard the supernatant by inverting the MagListo Magnetic Separation Rack. The silicone immobilizer inside the stand prevents the tubes from falling. When discarding the supernatant, invert the rack completely so that the solution does not to spill on the rack. 11

16 9. (1 st washing: 9-12) Detach the magnet plate from the MagListo Magnetic Separation Rack. Add 500 μl (mini) / 3 ml (midi) / 5 ml (maxi) of Buffer 3 (1 st Washing) to the each tube and close the cap. Mix using a vortex mixer until the beads are fully resuspended. - Detachment of the magnet plate Detach the magnet plate gently by pulling it upwards. 10. Attach the magnet plate and invert the rack gently 3 to 4 times until the beads bind tightly to the magnet. 11. Without removing the tubes from the MagListo Magnetic Separation Rack, discard the supernatant and remove the remaining supernatant on a paper towel by blotting action. 12. Repeat the steps 9-11 to remove remaining pigments and other debris. 13. (2 nd washing) Repeat the steps 9-11 by adding 700 μl (mini) / 5 ml (midi) / 10 ml (maxi) of Buffer 4 (2 nd Washing) instead of Buffer 3 for additional washing. 14. (3 rd washing) Without removing the tubes from the MagListo Magnetic Separation Rack, add 700 μl (micro, mini) / 8 ml (midi) / 15 ml (maxi) of Buffer 5 (3 rd Washing) to the opposite side of bead pellet close the cap and invert the rack twice in order to remove ethanol from the sample. (Note) Direct pipetting of Buffer 5 onto the bead pellet, vortexing and/or vigorous shaking of the tubes may release nucleic acid from the beads, which may result in lower DNA yield than expected. 15. Discard the supernatant and completely remove the remaining supernatant by blotting action. 12

17 - Add Buffer 5 and discard the supernatant 16. (Elution: 16-21) Detach the magnet plate from the MagListo Magnetic Separation Rack. Add 100 μl (mini) / 500 μl (midi) / 1 ml (maxi) of Buffer 6 (Elution) or distilled water to the each tube and resuspend the bead pellet completely by pipetting or using a vortex mixer for 15 sec. 17. Incubate the tubes at 60 for 1 min. 18. Vortex the tubes for 15 sec. 19. Place the tubes on the MagListo Magnetic Separation Rack, attach the magnet plate and invert the rack gently 3 to 4 times until the beads bind tightly to the magnet. 20. Without removing the tube from the MagListo Magnetic Separation Rack, transfer supernatant containing DNA carefully to a new sterile microcentrifuge tube. 21. Discard the tubes with the remaining Magnetic Nano Bead pellet. Do not reuse the beads. 13

18 Summary of Reagents Volume in Each Step of DNA Extraction from Whole Blood Step Buffer Mini Midi Maxi Page Blood (+ PBS) 200 μl 2 ml 4 ml P. 10 Lysis Buffer 2 (Binding) 200 μl 2 ml 4 ml P. 10 DNA Precipitation Absolute Ethanol 400 μl 4 ml 8 ml P. 10 Bead Binding Magnetic Nano Bead - DNA 100 μl 500 μl 1 ml P st Washing (Repeat) Buffer 3 (1 st Washing) 500 μl 3 ml 6 ml P nd Washing Buffer 4 (2 nd Washing) 700 μl 5 ml 10 ml P rd Washing Buffer 5 (3 rd Washing) 700 μl 8 ml 15 ml P. 12 Elution Buffer 6 (Elution) 100 μl 500 μl 1 ml P

19 B. DNA Extraction from Cultured Cells for Micro /Mini /Midi /Maxi scale 1. Centrifuge the cultured cells (~ 1x10 4 (micro) / ~ 1x10 6 (mini) / ~ 5x10 6 (midi) / ~ 1x10 7 (maxi)) for 10 min at 300 x g. Discard the supernatant carefully without disturbing the pellet. (Note) For cultured cells with lower cell number than 1x10 4, micro scale as described in this protocol. 2. Resuspend the pellet in 100 μl (micro) / 200 μl (mini) / 1 ml (midi, maxi) of 1X PBS and transfer the resuspended cells to each tube. a. (Micro / Mini) Transfer the resuspended cells to a new 1.5 ml or 2 ml tube. b. (Midi / Maxi) Transfer the resuspended cells to a new 15 ml tube. 3. Add 10 μl (micro) / 20 μl (mini) / 100 μl (midi) / 200 μl (maxi) of Proteinase K (see Before you begin ) to the each tube. 4. If you require RNA-free genomic DNA is required, add up to 2 μl (micro) / 10 μl (mini) / 75 μl (midi) / 150 μl (maxi) of RNase A (see Before you begin ) to each tube and incubate the tubes for 5 min at room temperature. 5. (Lysis: 5-6) Add 100 μl (micro) / 200 μl (mini) / 1 ml (midi, maxi) of Buffer 2 (Binding) to the each tube and mix thoroughly using a vortex mixer. Make sure that you completely resuspend the sample to achieve maximum lysis efficiency. 6. Incubate the tubes at 60 for 10min. 7. (DNA precipitation) Add 200 μl (micro) / 400 μl (mini) / 2 ml (midi, maxi) of absolute ethanol to each tube and mix well using a vortex mixer or by pipetting. 8. (DNA binding with Magnetic Nano Bead: 8-10) Add 100 μl (micro, mini) / 500 μl (midi) / 1 ml (maxi) of Magnetic Nano Bead solution to each tube and mix thoroughly using a vortex mixer until the beads are fully resuspended. 15

20 (Note) Magnetic Nano Bead Solution contains Magnetic Nano Beads. Please shake well or mix with a vortex mixer before use. 9. Place the tubes on the MagListo -2 (micro, mini) / MagListo -15 (midi, maxi) Magnetic Separation Rack with the magnet plate attached and invert the rack gently 3 to 4 times until the beads bind tightly to the magnet. - Attachment of the magnet plate Combine the magnet plate to the stand. 10. Without removing the tubes from the MagListo Magnetic Separation Rack, discard the supernatant carefully and completely remove the remaining supernatant on a paper towel by blotting action. - How to discard the supernatant Discard the supernatant by inverting the MagListo Magnetic Separation Rack. The silicone immobilizer inside the stand prevents the tubes from falling. When discarding the supernatant, invert the rack completely so that the solution does not spill on the rack. 11. (1 st washing: 11-13) Detach the magnet plate from the MagListo Magnetic Separation Rack. Add 700 μl (micro, mini) / 5 ml (midi) / 10 ml (maxi) of Buffer 3 (1 st Washing) to each tube and close the cap. Mix using a vortex mixer until the beads are fully resuspended. 16

21 - Detachment of the magnet plate Detach the magnet plate gently by pulling it upwards. 12. Attach the magnet plate and invert the rack gently 3 to 4 times until the beads bind tightly to the magnet. 13. Without removing the tubes from the MagListo Magnetic Separation Rack, discard the supernatant and remove the remaining supernatant on a paper towel by blotting action. 14. (2 nd washing) Repeat the steps by adding 700 μl (micro, mini) / 5 ml (midi) / 10 ml (maxi) Buffer 4 (2 nd Washing) instead of Buffer 3 for additional washing. 15. (3 rd washing) Without removing the tubes from the MagListo Magnetic Separation Rack, add 700 μl (micro, mini) / 8 ml (midi) / 15 ml (maxi) of Buffer 5 (3 rd Washing) to the opposite side of the bead pellet. Close the cap and gently invert the rack twice in order to remove ethanol from the sample. (Note) Direct pipetting of Buffer 5 onto the bead pellet, vortexing and/or vigorous shaking of the tubes may release nucleic acid from the beads, which may result in lower DNA yield than expected. 16. Discard the supernatant and completely remover the remaining supernatant by blotting action. - Add Buffer 5 and discard the supernatant 17

22 17. (Elution: 17-21) Detach the magnet plate from the MagListo Magnetic Separation Rack. Add 50 μl (micro) / 100 μl (mini) / 500 μl (midi) / 1 ml (maxi) of Buffer 6 (Elution) or distilled water to the each tube completely resuspend the bead pellet by pipetting or using a vortex mixer for 15 sec. 18. Incubate the tubes at 60 for 1 min. 19. Place the tubes on the MagListo Magnetic Separation Rack, attach the magnet plate and invert the track gently 3 to 4 times until the beads bind tightly to the magnet. 20. Without removing the tubes from the MagListo Magnetic Separation Rack, transfer supernatant containing DNA to a new sterile microcentrifuge tube. 21. Discard the tubes with remaining Magnetic Nano Bead pellet. Do not reuse the beads. 18

23 Summary of Reagents Volume in Each Step of DNA Extraction from Cultured Cell Step Buffer Micro Mini Midi Maxi Page Cultured Cell ~ 1 x 10 4 ~ 1 x 10 6 ~ 5 x 10 6 ~1 x 10 7 P. 15 Lysis Buffer 2 (Binding) 100 μl 200 μl 1 ml 1 ml P. 15 DNA Precipitation Absolute Ethanol 200 μl 400 μl 2 ml 2 ml P. 15 Bead Binding Magnetic Nano Bead - DNA 100 μl 100 μl 500 μl 1 ml P st Washing Buffer 3 (1 st Washing) 700 μl 700 μl 5 ml 10 ml P nd Washing Buffer 4 (2 nd Washing) 700 μl 700 μl 5 ml 10 ml P rd Washing Buffer 5 (3 rd Washing) 700 μl 700 μl 8 ml 15ml P. 17 Elution Buffer 6 (Elution) 50 μl 100 μl 500 μl 1 ml P

24 C. DNA Extraction from Animal Tissue for Mini /Midi /Maxi scale 1. (Homogenization) Disrupt (or homogenize) the tissue sample (~ 25 mg (mini) / ~ 100 mg (midi) / ~ 250 mg (maxi)) with a mortar and a pestle. Transfer disrupted or homogenized sample to each tube. a. (Mini) Transfer the homogenized tissue sample to a 1.5 ml or 2 ml tube. b. (Midi) Transfer the homogenized tissue sample to a 15 ml tube. c. (Maxi) Transfer the homogenized tissue sample to a 50 ml tube. (Note) Immediately place the weighted, fresh or frozen tissue in liquid nitrogen and grind to a fine powder with a mortar and a pestle. Final yield of DNA will depend on the amount and the type of the tissue used. 2. (Lysis: 2-6) Add 180 μl (mini) / 1.8 ml (midi) / 3.6 ml (maxi) of Buffer l (Lysis) to each tube. 3. Add 20 μl (mini) / 100 μl (midi) / 200 μl (maxi) of Proteinase K (see Before you begin ) to the each tube and mix thoroughly using a vortex mixer. 4. If you require RNA-free genomic DNA is required, add up to 10 μl (mini) / 75 μl (midi) / 150 μl (maxi) of RNase A (see Before you begin ) and incubate the tubes for 2 min at room temperature. 5. Incubate the tubes at 60 until the tissue is completely lysed. 6. Add 200 μl (mini) / 2 ml (midi) / 4 ml (maxi) of Buffer 2 (Binding) to the each tube and mix thoroughly using a vortex mixer. Make sure that you completely resuspend the sample to achieve maximum lysis efficiency. 7. (DNA precipitation) Add 400 μl (mini) / 4 ml (midi) / 8 ml (maxi) of absolute ethanol to each tube and mix well using a vortex mixer or by pipetting. 8. (DNA binding with Magnetic Nano Bead: 8-10) Add 100 μl (mini) / 500 μl (midi) / 1 ml (maxi) of Magnetic Nano Bead solution to the each tube and mix thoroughly using a vortex mixer until the beads are fully resuspended. 20

25 (Note) Magnetic Nano Bead solution contains Magnetic Nano Beads. Please shake well or mix using a vortex mixer before use. 9. Place the tubes on the MagListo -2 (mini) / MagListo -15 (midi) / MagListo -50 (maxi) Magnetic Separation Rack with the magnet plate and invert the rack gently 3 to 4 times until the beads bind tightly to the magnet. - Attachment of the magnet plate Combine the magnet plate to the stand. 10. Without removing the tubes from the MagListo Magnetic Separation Rack, discard the supernatant carefully and completely remove the remaining supernatant using a paper towel by blotting action. - How to discard the supernatant Discard the supernatant by inverting the MagListo Magnetic Separation Rack. The silicone immobilizer inside the stand prevents the tubes from falling. When discarding the supernatant, invert the rack completely so that the solution does not spill on the rack. 11. (1 st washing: 11-13) Detach the magnet plate from the MagListo Magnetic Separation Rack. Add 700 μl (mini) / 5 ml (midi) / 10 ml (maxi) of Buffer 3 (1 st Washing) to each tube and close the cap. Mix using a vortex mixer until the beads are fully resuspended. 21

26 - Detachment of the magnet plate Detach the magnet plate gently by pulling it upwards. 12. Attach the magnet plate and invert the rack gently 3 to 4 times until the beads bind tightly to the magnet. 13. Without removing the tubes from the MagListo Magnetic Separation Rack, discard the supernatant and remove the remaining supernatant on a paper towel by blotting action. 14. (2 nd washing) Repeat the steps by adding 700 μl (mini) / 5 ml (midi) / 10 ml (maxi) Buffer 4 (2 nd Washing) instead of Buffer 3 for additional washing. 15. (3 rd washing) Without removing the tubes from MagListo Magnetic Separation Rack, add 700 μl (micro, mini) / 8 ml (midi) / 15 ml (maxi) of Buffer 5 (3 rd Washing) to the opposite side of the bead pellet. Close the cap and invert the rack twice in order to remove ethanol from the sample. (Note) Direct pipetting of Buffer 5 onto the bead pellet, vortexing or vigorous shaking of the tubes may release nucleic acid from the beads, which may result in lower DNA yield than expected. 16. Discard the supernatant and completely remove the remaining supernatant by blotting action. - Add Buffer 5 and discard the supernatant 22

27 17. (Elution: 17-21) Detach the magnet plate from the MagListo Magnetic Separation Rack. Add 100 μl (mini) / 500 μl (midi) / 1 ml (maxi) of Buffer 6 (Elution) or distilled water to each tube and completely resuspend the bead pellet by pipetting or using a vortex mixer for 15 sec. 18. Incubate the tubes at 60 for 1 min. 19. Place the tubes on the MagListo Magnetic Separation Rack, attach the magnet plate and invert the rack gently 3 to 4 times until the beads bind tightly to the magnet. 20. Without removing the tubes from MagListo Magnetic Separation Rack, transfer supernatant containing DNA carefully to a new sterile microcentrifuge tube. 21. Discard the tubes with the remaining Magnetic Nano Bead pellet. Do not reuse the beads. 23

28 Summary of Reagents Volume in Each Step of DNA Extraction from Animal Tissue Step Buffer Mini Midi Maxi Page Animal tissue ~ 25 mg ~ 100 mg ~ 250 mg P. 20 Lysis Buffer l (Lysis) 180 μl 1.8 ml 3.6 ml P. 20 Buffer 2 (Binding) 200 μl 2 ml 4 ml P. 20 DNA Precipitation Absolute Ethanol 400 μl 4 ml 8 ml P. 20 Bead Binding Magnetic Nano Bead DNA 100 μl 500 μl 1 ml P st Washing Buffer 3 (1 st Washing) 700 μl 5 ml 10 ml P nd Washing Buffer 4 (2 nd Washing) 700 μl 5 ml 10 ml P rd Washing Buffer 5 (3 rd Washing) 700 μl 8 ml 15 ml P. 22 Elution Buffer 6 (Elution) 100 μl 500 μl 1 ml P

29 D. DNA Extraction from Bacterial Cells (Gram-Negative Bacteria) for Mini/Midi/Maxi 1. (Cell collection) Collect the bacterial cells (~1x10 9 (mini) / ~5x10 9 (midi) / ~1x10 10 (maxi)) by centrifuging at 6000 x g (> 8,000 rpm in a microcentrifuge) for 10 min (or >3,500 rpm for 20 min). Discard the supernatant (media) by using a pipette. 2. (Resuspension of cell pellet: 2-3) Add 180 μl (mini) / 1.8 ml (midi) / 3.6 ml (maxi) of Buffer l (Lysis) to the collected cell pellet and completely resuspend using a vortex mixer or by pipetting. 3. Transfer the cell suspension to each tube. a. (Mini) Transfer the cell suspension to a 1.5 ml or 2 ml tube. b. (Midi) Transfer the cell suspension to a 15 ml tube. c. (Maxi) Transfer the cell suspension to a 50 ml tube. 4. Go to step 3 of C. DNA Extraction from Animal Tissue in page 20 and follow the instructions accordingly. 25

30 E. DNA Extraction from Bacterial Cells (Gram-Positive Bacteria) for Mini/Midi/Maxi 1. (Cell collection) Collect the bacterial cells (~ 1x10 9 (mini) / ~ 5x10 9 (midi) / ~ 1x10 10 (maxi)) by centrifuging at 6,000 x g (>8,000 rpm in a microcentrifuge) for 10 min (or >3,500 rpm for 20 min). Discard the supernatant (media) by using a pipette. 2. (Resuspension of cell pellet: 2-3) Add 180 μl (mini) / 1.8 ml (midi) / 3.6 ml (maxi) of Lysis Buffer for Gram-Positive bacteria (not provided) to the collected cell pellet and completely resuspend using a vortex mixer or by pipetting. (Note) Lysis Buffer for Gram-Positive bacteria can be prepared by using this formulation: 20 mm Tris- HCl (ph8.0), 2 mm sodium EDTA and 1.2% Triton X Transfer the cell suspension to each tube. a. (Mini) Transfer the cell suspension to a 1.5 ml or 2 ml tube. b. (Midi) Transfer the cell suspension to a 15 ml tube. c. (Maxi) Transfer the cell suspension to a 50 ml tube. 4. (Lysis: 4-9) Add 20 μl (mini) / 100 μl (midi) / 200 μl (maxi) of lysozyme (100 mg/ml, not provided) to each tube and mix thoroughly using a vortex mixer. 5. If you require RNA-free genomic DNA, add up to 10 μl (mini) / 75 μl (midi) / 150 μl (maxi) of RNase A (see Before you begin ). 6. Incubate the tubes at 37 for 30 min. 7. Add 20 μl (mini) / 100 μl (midi) / 200 μl (maxi) of Proteinase K (see Before you begin ) to each tube. 8. Add 200 μl (mini) / 2 ml (midi) / 4 ml (maxi) of Buffer 2 (Binding) to each tube and mix thoroughly using a vortex mixer. 9. Incubate the tubes at 60 for 30 min or until bacterial cells are completely lysed. 10. Go to step 7 of C. DNA Extraction from Animal Tissue in page 20 and follow the instructions accordingly. 26

31 F. DNA Clean-Up (DNA Purification) 1. Transfer the eluted DNA or enzyme reaction product to a new sterile tube. a. (Mini) Transfer the eluate to a 1.5 ml or 2 ml tube b. (Midi / Maxi) Transfer the eluate to a 15 ml tube 2. If you require RNA-free genomic DNA, add up to 10 μl (mini) / 75 μl (midi) / 150 μl (maxi) of RNase A (see Before you begin ) and incubate the tubes for 2 min at room temperature. 3. (Binding) Add 1 volume of Buffer 2 (Binding) to 1 volume of the eluted DNA and mix completely using a vortex mixer. 4. (DNA precipitation) Add 3 volumes of absolute ethanol to 1 volume of the eluted DNA and mix well using a vortex mixer. 5. (DNA binding with Magnetic Nano Bead: 5-7) Add 100 μl (mini) / 500 μl (midi) / 1 ml (maxi) of Magnetic Nano Bead solution to each tube and mix thoroughly using a vortex mixer until the beads are fully resuspended. (Note) Magnetic Nano Bead Solution contains Magnetic Nano Beads. Please shake well or mix with a vortex mixer before use. 6. Place the tubes on the MagListo -2 (mini) / MagListo -15 (midi, maxi) Magnetic Separation Rack with the magnet plate attached and invert the rack gently 3 to 4 times until the beads bind tightly to magnet. - Attachment of the magnet plate Combine the magnet plate to the stand. 27

32 7. Without removing the tubes from the MagListo Magnetic Separation Rack, discard the supernatant carefully and completely remove the remaining supernatant on a paper towel by blotting action. - How to discard the supernatant Discard the supernatant by inverting the MagListo Magnetic Separation Rack. The silicone immobilizer inside the stand prevents the tubes from falling. When discarding the supernatant, invert the rack completely so that the solution does not spill on the rack. 8. (1 st washing: 8-10) Detach the magnet plate from the MagListo Magnetic Separation Rack. Add 700 μl (mini) / 5 ml (midi) / 10 ml (maxi) of Buffer 4 (2 nd Washing) to each tube and close the cap. Mix with a vortex mixer until the beads are fully resuspended. - Detachment of the magnet plate Detach the magnet plate gently by pulling it upwards. 9. Attach the magnet plate and invert the rack gently 3 to 4 times until the beads bind tightly to magnet. 10. Without removing the tubes from MagListo Magnetic Separation Rack, discard the supernatant and remove the remaining supernatant on a paper towel by blotting action. 11. Go to step 15 of C. DNA Extraction from Animal Tissue in page 22 and follow the instructions accordingly. 28

33 XVII. Appendix Troubleshooting guide If you have problems during genomic DNA extraction, please use the Troubleshooting Guide. This troubleshooting guide will help you to solve problem that may arise during DNA extraction. For other technical assistance or more information, please contact our technical assistance team. Comments and suggestions Buffers or other reagents may have been exposed to external factors that may have reduced its quality. Please make sure that reagents are stored at room temperature at all times upon arrival and that all reagent bottles are closed tightly, in order to preserve ph and stability, and to avoid contamination. Cells may not have been lysed properly, especially in the case of tissue cells. Please, ensure that the turbid sample changes to a clear solution, indicating that the protein digestion has occurred. Extend the incubation time if tissue cells are still not lysed. The amount of time for complete lysis varies depending on the Low yield of DNA type of tissue or sample type used. If a cell mass still remains after the overnight incubation, centrifuge the sample and use supernatant for DNA extraction. A shaking water bath should be used for efficient cell lysis. Excess amount of starting sample was used to extract DNA. Appropriate amount of starting sample (see Kit Specification in page 4) should be used for efficient extraction of genomic DNA. Elution may have been incomplete. Please extend incubation time up to 3 minutes at elution step to improve the DNA. In addition, make sure that Magnetic Nano Beads are suspended completely in the eluting solution during incubation. 29

34 Some of Magnetic Nano Bead pellet may have been lost while discarding solution. Check that all of the Nano Beads have bound tightly to the magnet when you discard supernatant. Insufficient shaking or vortexing during lysis step may lead to low DNA yield. Shake or mix with a vortex mixer sufficiently during incubation step. Beads may have been washed insufficiently. You must properly wash the beads in the 3 rd washing step. Remained ethanol can decrease the purity of DNA. Take Low A 260/280 ratio enough time to properly wash the beads. Incomplete suspension of beads during the washing step causes salts to remain in the purified DNA. Make sure that the beads are suspended thoroughly during the washing process. RNA may be present in the eluted DNA when both DNA and RNA are resent in Presence of RNA in the eluted DNA the sample. If you require RNA-free genomic DNA, add RNase A should be added to the sample before addition of Buffer 2 (Binding). If you wish to RNA from the eluted DNA, please refer to F. DNA Clean-Up (DNA Purification) protocol in page 20 for details. Excessively clustered Magnetic Nano Bead Excess amount of starting sample is used to extract DNA. Appropriate amount of starting material (see Kit Specification in page 4) should be used for efficient extraction of genomic DNA. Presence of a white precipitate in buffers A white precipitate may form in Buffer l (Lysis) or Buffer 2 (Binding) due to prolonged storage at low temperatures. Incubate Buffer l (Lysis) or Buffer 2 (Binding) at 60 to dissolve any precipitate in the buffer. 30

35 The DNA from old or incorrectly stored sample may often be degraded. As the DNA yield is highly dependent on storage conditions of samples, please use fresh samples for optimal results. In case of using stored tissue sample, it is Degraded DNA recommended to use sample stored at -70. Frequent freezing and thawing may result in lower DNA yield than expected. Avoid repeated freezing and thawing. Flotation of extracted DNA when loaded on an agarose gel Floating of DNA on an agarose gel is caused by the remaining ethanol in the eluted DNA. Ensure that the 3 rd Washing (ethanol removing) step in the protocol is properly performed. Remaining ethanol may also interrupt the enzymatic reaction. 31

36 Experimental data Figure1. Comparison of extracted genomic DNA from human whole blood (200μl) purified with MagListo 5M Genomic DNA Extraction Kit and competitor s kit (Single Column Type) M M: Bioneer 1kb ladder 1-4: Extracted blood genomic DNA purified with Bioneer MagListo 5M Genomic DNA Extraction Kit 5-7: Extracted blood genomic DNA purified with competitor Q kit Sample Total Yield (ug) A260/A280 A260/A Figure2. Comparison of extracted genomic DNA from HeLa cell (1x10 6 ) purified with MagListo 5M Genomic DNA Extraction Kit and competitor s kit (Single Column Type) M M: Bioneer 1kb ladder 1-4: Extracted cell genomic DNA purified with Bioneer MagListo 5M Genomic DNA Extraction Kit 5-6: Extracted cell genomic DNA purified with competitor Q kit Sample Total Yield (ug) A260/A280 A260/A

37 Figure3. Comparison of extracted genomic DNA from bovine skeletal muscle tissue (15mg) purified with MagListo 5M Genomic DNA Extraction Kit and competitor s kit (Single Column Type) M M: Bioneer 1kb ladder 1-4: Extracted tissue genomic DNA purified with Bioneer MagListo 5M Genomic DNA Extraction Kit 5-6: Extracted tissue genomic DNA purified with competitor Q kit Sample Total Yield (μg) A260/A280 A260/A Figure4. Comparison of extracted genomic DNA from E. coli purified with MagListo 5M Genomic DNA Extraction Kit and competitor s kit (Single Column Type) M M: Bioneer 1kb ladder 1-2: (Mini) Extracted genomic DNA from E.coli (1x10 9 ) purified with Bioneer MagListo 5M Genomic DNA Extraction Kit 3-4: (Mini) Extracted genomic DNA from E.coli (1x10 9 ) purified with competitor Q kit 5-6: (Midi) Extracted genomic DNA from E.coli (5x10 9 ) 7: (Maxi) Extracted genomic DNA from E.coli (1x10 10 ) Sample Total Yield (ug) A260/A280 A260/A

38 Figure5. Comparison of extracted genomic DNA from B. subtilis purified with MagListo 5M Genomic DNA Extraction Kit and competitor s kit (Single Column Type) M M: Bioneer 1kb ladder 1-2: (Mini) Extracted genomic DNA from B. subtilis (1x10 9 ) purified with Bioneer MagListo 5M Genomic DNA Extraction Kit 3-4: (Mini) Extracted genomic DNA from B. subtilis (1x10 9 ) purified with competitor Q kit 5: (Midi) Extracted genomic DNA from B. subtilis (5x10 9 ) 6: (Maxi) Extracted genomic DNA from B. subtilis (1x10 10 ) Sample Total Yield (μg) A260/A280 A260/A Figure6. Comparison of Real Time qpcr data of extracted genomic DNA from HeLa cell (1x10 1 ~1x10 5 ) purified with MagListo 5M Genomic DNA Extraction Kit and competitor s kit (Single Column Type) 34

39 XVIII. Ordering Information Cat no. Product Description Size K-3601 MagListo TM 5M Plamid Extraction Kit, 100 reactions (mini) 1 kit K-3600 MagListo TM 5M Plamid Extraction Kit, 500 reactions (mini) 1 kit K-3603 MagListo TM 5M Genomic DNA Extraction Kit, 100 reactions (mini) 1 kit K-3605 MagListo TM 5M Plant Genomic DNA Extraction Kit, 100 reactions (mini) 1 kit K-3607 MagListo TM 5M Gel Extraction Kit, 100 reactions (mini) 1 kit K-3609 MagListo TM 5M PCR Purification Kit, 100 reactions (mini) 1 kit K-3611 MagListo TM 5M Cell Total RNA Extraction Kit, 100 reactions (mini) 1 kit K-3613 MagListo TM 5M Tissue Total RNA Extraction Kit, 100 reactions (mini) 1 kit K-3615 MagListo TM 5M Forensic Sample DNA Extraction Kit, 100 reactions (mini) 1 kit K-3617 MagListo TM 5M Viral DNA / RNA Extraction Kit, 100 reactions (mini) 1 kit K-3619 MagListo TM 5M Circulating Cell Free DNA Extraction Kit, 50 reactions (mini) 1 kit TM-1000 MagListo TM -8Ch Magnetic Separation Rack 1 ml tube x 8 holes TM-1010 MagListo TM -2 Magnetic Separation Rack 2 ml tube x 8 holes TM-1020 MagListo TM -15 Magnetic Separation Rack 15 ml tube x 6 holes TM-1030 MagListo TM -50 Magnetic Separation Rack 50 ml tube x 3 holes HT-15-NG 1.5 ml microcentrifuge tube 500 ea / pk HT-20-NG 2 ml microcentrifuge tube 500 ea / pk 35

40 XIX. Explanation Symbols Catalog Number Batch code Manufacturer Contains sufficient for (n) tests Caution, consult accompanying documents Caution, Potential Biohazard USE BY Temperature Limitation DO NOT REUSE Consult Instruction For Use 36

41

Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr Contents I. Overview II. Kit components III.

More information

Version No.: 2.0 ( )

Version No.: 2.0 ( ) Kit for the extraction of fragment DNA from agarose gel using MagListo Version No.: 2.0 (2017-03) Please read all the information in booklet before using the unit Bioneer Corporation 8-11, Munpyeongseo-ro,

More information

Version No.: 2.0 ( )

Version No.: 2.0 ( ) Kit for the extraction of genomic DNA from plant using MagListo Version No.: 2.0 (2017-03) Please read all the information in booklet before using the unit Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu,

More information

Version No.: 2.0 ( )

Version No.: 2.0 ( ) Kit for the extraction of total RNA from wide range of tissue using MagListo Version No.: 2.0 (2017-03) Please read all the information in booklet before using the unit Bioneer Corporation 8-11, Munpyeongseo-ro,

More information

Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr Contents I. Overview 1 II. Kit Components 1

More information

Contents. I. Overview 1. II. Kit Components 1. III. Storage 2. IV. Intended Use 2. V. Safety Warnings and Precautions 2. VI. Warranty and Liability 2

Contents. I. Overview 1. II. Kit Components 1. III. Storage 2. IV. Intended Use 2. V. Safety Warnings and Precautions 2. VI. Warranty and Liability 2 Contents I. Overview 1 II. Kit Components 1 III. Storage 2 IV. Intended Use 2 V. Safety Warnings and Precautions 2 VI. Warranty and Liability 2 VII. Technical Assistance 3 VIII. Quality Management 3 IX.

More information

MagListo 5M Tissue Total RNA Extraction Kit

MagListo 5M Tissue Total RNA Extraction Kit User s Guide MagListo 5M Tissue Total RNA Extraction Kit K-3612 K-3613 MagListo TM 5M Tissue Total RNA Extraction Kit MagListo 5M Tissue Total RNA Extraction Kit Kit for the extraction of total RNA from

More information

MagListo 5M Cell Total RNA Extraction Kit

MagListo 5M Cell Total RNA Extraction Kit User s Guide MagListo 5M Cell Total RNA Extraction Kit K-3610 K-3611. MagListo 5M Cell Total RNA Extraction Kit Kit for the extraction of total RNA from wide range of cell types using MagListo User s Guide

More information

AccuPrep Genomic DNA Extraction Kit

AccuPrep Genomic DNA Extraction Kit User s Guide AccuPrep Genomic DNA Extraction Kit K-3032 User s Guide 100 Version No.: 3.0 (2018-01) Please read all the information in booklet before using the unit Bioneer Corporation 8-11,Munpyeongseo-ro,

More information

Please read all the information in booklet before using the unit

Please read all the information in booklet before using the unit 100 Please read all the information in booklet before using the unit Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email:

More information

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: 100 Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions

More information

Version No.: 2.0 ( )

Version No.: 2.0 ( ) Kit for the extraction of plasmid from bacterial cultures using MagListo Version No.: 2.0 (2017-03) Please read all the information in booklet before using the unit Bioneer Corporation 8-11, Munpyeongseo-ro,

More information

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: 100 Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions

More information

RayBio Genomic DNA Magnetic Beads Kit

RayBio Genomic DNA Magnetic Beads Kit RayBio Genomic DNA Magnetic Beads Kit Catalog #: 801-112 User Manual Last revised January 4 th, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100

More information

AccuPrep Plant Genomic DNA Extraction Kit

AccuPrep Plant Genomic DNA Extraction Kit User s Guide AccuPrep Plant Genomic DNA Extraction Kit K-3031 AccuPrep Plant Genomic DNA Extraction Kit User's Guide 100 Version No.: 2.0 (2018-02) Please read all the information in booklet before using

More information

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions

More information

ExiPrep TM Plus Genomic DNA Kit

ExiPrep TM Plus Genomic DNA Kit User s Guide ExiPrep TM Plus Genomic DNA Kit Cat. No.: K-4211 - K-4215 ExiPrep TM Plus Blood Genomic DNA Kit (K-4211) ExiPrep TM Plus Tissue Genomic DNA Kit (K-4212) ExiPrep TM Plus Cell Genomic DNA Kit

More information

DNA Purification Magnetic Beads

DNA Purification Magnetic Beads DNA Purification Magnetic Beads Catalog #: 801-109 User Manual Last revised December 17 th, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,

More information

Genomic DNA Magnetic Bead Kit KOA091 Rockland s Genomic DNA Magnetic Bead Kit is designed to purify genomic DNA from mammalian tissues and bacteria. Paramagnetic beads with uniform particle size efficiently

More information

Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) Product # 62500

Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) Product # 62500 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System)

More information

Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr Safety Warnings and Precautions ExiPrep TM Plus

More information

EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK

EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 8 Rev 05/0/03 EZ-0 Genomic DNA Kit Handbook Table of Contents Introduction Limitations of Use Features Applications

More information

Cells and Tissue DNA Isolation Kit (Magnetic Bead System) 50 Preps Product # 59100

Cells and Tissue DNA Isolation Kit (Magnetic Bead System) 50 Preps Product # 59100 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cells and Tissue DNA Isolation Kit (Magnetic Bead System) 50 Preps

More information

Tissue & Cell Genomic DNA Purification Kit. Cat. #:DP021/ DP Size:50/150 reactions Store at RT For research use only

Tissue & Cell Genomic DNA Purification Kit. Cat. #:DP021/ DP Size:50/150 reactions Store at RT For research use only Tissue & Cell Genomic DNA Purification Kit Cat. #:DP021/ DP021-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Tissue & Cell Genomic DNA Purification Kit provides a rapid,

More information

Kit Components Product # (50 samples) Wash Solution A Elution Buffer B

Kit Components Product # (50 samples) Wash Solution A Elution Buffer B 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cells and Tissue DNA Isolation Kit Product # 53100 Product Insert

More information

Presto Mini gdna Bacteria Kit

Presto Mini gdna Bacteria Kit Instruction Manual Ver. 02.10.17 For Research Use Only Presto Mini gdna Bacteria Kit Advantages GBB004 (4 Preparation Sample Kit) GBB100/101 (100 Preparation Kit) GBB300/301 (300 Preparation Kit) Sample:

More information

DNA isolation from tissue DNA isolation from eukaryotic cells (max. 5 x 106 cells) DNA isolation from paraffin embedded tissue

DNA isolation from tissue DNA isolation from eukaryotic cells (max. 5 x 106 cells) DNA isolation from paraffin embedded tissue INDEX KIT COMPONENTS 3 STORAGE AND STABILITY 3 BINDING CAPACITY 3 INTRODUCTION 3 IMPORTANT NOTES 4 EUROGOLD TISSUE DNA MINI KIT PROTOCOLS 5 A. DNA isolation from tissue 5 B. DNA isolation from eukaryotic

More information

Plus Blood Genomic DNA Purification Kit

Plus Blood Genomic DNA Purification Kit Plus Blood Genomic DNA Purification Kit Cat #:DP023P/ DP023P-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Plus Blood Genomic DNA Purification Kit provides a rapid, simple

More information

Total RNA Purification Kit. Cat. #.: TR01 / TR Size : 50 / 150 Reactions Store at RT For research use only

Total RNA Purification Kit. Cat. #.: TR01 / TR Size : 50 / 150 Reactions Store at RT For research use only Total RNA Purification Kit Cat. #.: TR01 / TR01-150 Size : 50 / 150 Reactions Store at RT For research use only 1 Description: The Total RNA Purification Kit provides a rapid, simple and effective approach

More information

Easy Tissue & Cell Genomic DNA Purification Kit. Cat. #:DP021E/ DP021E-150 Size:50/150 reactions Store at RT For research use only

Easy Tissue & Cell Genomic DNA Purification Kit. Cat. #:DP021E/ DP021E-150 Size:50/150 reactions Store at RT For research use only Easy Tissue & Cell Genomic DNA Purification Kit Cat. #:DP021E/ DP021E-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Easy Tissue & Cell Genomic DNA Purification Kit is ideal

More information

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:

Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax: Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions

More information

Presto 96 Well gdna Bacteria Kit

Presto 96 Well gdna Bacteria Kit Presto 96 Well gdna Bacteria Kit 96GBB02 (2 x 96 well plates/kit) 96GBB04 (4 x 96 well plates/kit) 96GBB10 (10 x 96 well plates/kit) Instruction Manual Ver. 05.04.17 For Research Use Only Advantages Sample:

More information

Bacteria Genomic DNA Purification Kit

Bacteria Genomic DNA Purification Kit Bacteria Genomic DNA Purification Kit Cat #:DP025/ DP025-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Bacteria Genomic DNA Purification Kit provides a rapid, simple, and

More information

HighPrep Blood & Tissue DNA Kit

HighPrep Blood & Tissue DNA Kit MAGBIO ACCELERATING genomic research HighPrep Blood & Tissue DNA Kit Manual Revision v1.1 Catalog Nos. HPBTS-D16, HPBTS-D96, HPBTS-D96X4 Genomic DNA isolation from 20-250 μl of blood, lysate of tissues,

More information

Total RNA Miniprep Purification Kit. Cat.# :TR01/TR Size : 50/150 Reactions Store at RT

Total RNA Miniprep Purification Kit. Cat.# :TR01/TR Size : 50/150 Reactions Store at RT Total RNA Miniprep Purification Kit Cat.# :TR01/TR01-150 Size : 50/150 Reactions Store at RT 1 Description: The Total RNA Miniprep Purification Kit provides a rapid, simple and effective approach to isolate

More information

DNA Isolation Reagent for Genomic DNA

DNA Isolation Reagent for Genomic DNA DNA Isolation Reagent for Genomic DNA Phenol-free solution for rapid isolation of genomic DNA Product-No. A3418 Description DNA Isolation Reagent for Genomic DNA is a non-organic and ready-to-use reagent

More information

AccuPrep GMO DNA Extraction Kit Cat. No.: K-3031

AccuPrep GMO DNA Extraction Kit Cat. No.: K-3031 AccuPrep GMO DNA Extraction Kit Cat. No.: K-3031 V109C3 Safety Warnings and Precautions. For research use only. It is not recommended to diagnose disease in humans or animals. DO NOT use to humans or animals.

More information

Blood DNA Isolation 96-Well Kit (Magnetic Bead System) Product # 62600

Blood DNA Isolation 96-Well Kit (Magnetic Bead System) Product # 62600 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Blood DNA Isolation 96-Well Kit (Magnetic Bead System) Product

More information

E.Z.N.A. mirna Kit. R preps R preps R preps

E.Z.N.A. mirna Kit. R preps R preps R preps E.Z.N.A. mirna Kit R7034-00 5 preps R7034-01 50 preps R7034-02 200 preps August 2011 E.Z.N.A. Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Plant DNA Isolation Kit (Magnetic Bead System) 50 Preps Product # 58200

Plant DNA Isolation Kit (Magnetic Bead System) 50 Preps Product # 58200 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plant DNA Isolation Kit (Magnetic Bead System) 50 Preps Product

More information

Presto 96 Well DNA Bacteria Advanced Kit

Presto 96 Well DNA Bacteria Advanced Kit Instruction Manual Ver. 07.04.17 For Research Use Only Presto 96 Well DNA Bacteria Advanced Kit 96GBBA02 (2 x 96 well plates/kit) 96GBBA04 (4 x 96 well plates/kit) 96GBBA10 (10 x 96 well plates/kit) Advantages

More information

LaboPass TM Blood mini

LaboPass TM Blood mini LaboPass TM Blood mini Protocol Book LaboPass TM Blood Mini Introduction LaboPass TM Blood Mini Kit provides a fast and convenient method for the isolation of total DNA from up to 400μLof fresh and frozen

More information

CAT. NO. BR BR BR SIZE 10 preps 50 preps 250 preps. Buffer LYSIS LC 12 ml 25 ml 120 ml. Buffer LYSIS LD 10 ml 25 ml 125 ml

CAT. NO. BR BR BR SIZE 10 preps 50 preps 250 preps. Buffer LYSIS LC 12 ml 25 ml 120 ml. Buffer LYSIS LD 10 ml 25 ml 125 ml Product Insert GenUP Plant DNA Kit LOT: See product label EXPIRY DATE: See product label ORDERING INFORMATION PRODUCT GenUP Plant DNA Kit CAT. NO. BR0700801 BR0700802 BR0700803 SIZE 10 preps 50 preps 250

More information

Genomic DNA Mini Kit (Blood/Cultured Cell) For research use only

Genomic DNA Mini Kit (Blood/Cultured Cell) For research use only Genomic DNA Mini Kit (Blood/Cultured Cell) For research use only Sample : up to 300 µl of whole blood, up to 200 µl of frozen blood, up to 200 µl of buffy coat, cultured animal cells (up to 1 x 107), 9

More information

AccuPrep Stool DNA Extraction Kit. Cat. No.: K-3036

AccuPrep Stool DNA Extraction Kit. Cat. No.: K-3036 AccuPrep Stool DNA Extraction Kit Cat. No.: K-3036 Safety Warnings and Precautions. For research use only. It is not recommended to diagnose disease in humans or animals. DO NOT use to humans or animals.

More information

Presto Mini Plasmid Kit

Presto Mini Plasmid Kit Instruction Manual Ver. 03.06.17 For Research Use Only Presto Mini Plasmid Kit PDH004 (4 Preparation Sample Kit) PDH100 (100 Preparation Kit) PDH300 (300 Preparation Kit) Advantages Sample: 1-7 ml of cultured

More information

MD60002 MD MD62002

MD60002 MD MD62002 MagSi-DNA saliva Art.No. MD60002 MD61002 - MD62002 Product Manual Version 1.0 22/01/2015 Table of Contents 1. General Information...3 1.1 Intended Use...3 1.2 Kit specifications...3 1.3 Basic principle...3

More information

Product # 24700, 24750

Product # 24700, 24750 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Genomic DNA Isolation Kit Product # 24700, 24750 Product Insert

More information

HiYield TM Genomic DNA Extraction Kit Reagent

HiYield TM Genomic DNA Extraction Kit Reagent HiYield TM Genomic DNA Extraction Kit Reagent CONTENTS Genomic DNA Extraction Kit Blood Cat.No. YGBE1KR // YGBE 100R Blood Protocol..... 3 Genomic DNA Extraction Kit Cultured Cells /Tissue / Bacterial

More information

EZ-DNA. Instructions for Use. Genomic DNA Isolation Reagent. Product Description. Kit Reagent. Reagent Required But Not Supplied.

EZ-DNA. Instructions for Use. Genomic DNA Isolation Reagent. Product Description. Kit Reagent. Reagent Required But Not Supplied. EZ-DNA Genomic DNA Isolation Reagent Cat. No.: 20-600-50 Store at: Room Temperature Instructions for Use Protocol for Genomic DNA Isolation Tissue Specific Recommendations for the Use of EZ-DNA Assessing

More information

EasyPrep TM Plant Genomic DNA. Miniprep Manual. Table of Contents. Introduction Kit Contents... 4

EasyPrep TM Plant Genomic DNA. Miniprep Manual. Table of Contents. Introduction Kit Contents... 4 EasyPrep TM Plant Genomic DNA Miniprep Manual Catalog#: GD02-01, GD02-02 Table of Contents Introduction... 3 Kit Contents... 4 Protocols A. Fresh/Frozen Specimens... 5 B. Dry Specimens... 8 C. Plant rich

More information

TaKaRa MiniBEST Universal RNA Extraction Kit

TaKaRa MiniBEST Universal RNA Extraction Kit Cat. # 9767 For Research Use TaKaRa MiniBEST Universal RNA Extraction Kit Product Manual Table of Contents I. Description...3 II. III. IV. Kit Components...3 Storage and Shipping...4 Precautions for Preventing

More information

Kit for DNA isolation from whole blood, plasma, serum, buffy coat, lymphocytes and body fluids

Kit for DNA isolation from whole blood, plasma, serum, buffy coat, lymphocytes and body fluids Cat. No. EM05 Version: 1.2014 Kit for DNA isolation from whole blood, plasma, serum, buffy coat, lymphocytes and body fluids EXTRACTME is a registered trademark of BLIRT S.A. www.dnagdansk.com 2 Cat. No.

More information

EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK

EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 5.0 Rev 03/25/205 Table of Contents Introduction 2 Limitations of Use 2 Features 2 Applications 2 Storage 2

More information

Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit #K0791, #K0792

Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit #K0791, #K0792 PRODUCT INFORMATION Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit #K0791, #K0792 Pub. No. MAN0016131 Rev. Date 12 October 2016 (Rev. A.00) Read Storage information (p. 2) before first

More information

Introduction 2. Storage and Stability Kit Contents Safety Information.. 3. Before Starting... 3

Introduction 2. Storage and Stability Kit Contents Safety Information.. 3. Before Starting... 3 Contents Introduction 2 Storage and Stability... 2 Kit Contents... 2 Safety Information.. 3 Before Starting.... 3 Disruption and Homogenization of Samples 4 Removal of Genomic DNA. 5 Stabilization of RNA

More information

FavorPrep Blood / Cultured Cell Genomic DNA Extraction Maxi Kit. User Manual

FavorPrep Blood / Cultured Cell Genomic DNA Extraction Maxi Kit. User Manual TM FavorPrep Blood / Cultured Cell Genomic DNA Extraction Maxi Kit User Manual Cat. No.: FABGK 003 (10 Preps) FABGK 003-1 (24 Preps) For Research Use Only v.1005 Introduction TM FavorPrep Genomic DNA Extraction

More information

DNA/RNA/Protein Extraction Kit

DNA/RNA/Protein Extraction Kit Instruction Manual Ver. 05.08.17 For Research Use Only DNA/RNA/Protein Extraction Kit IB47700 (4 Preparation Sample Kit) IB47701 (50 Preparation Kit) IB47702 (100 Preparation Kit) Advantages Sample: cultured

More information

ISOLATE II Blood DNA Kit. Product Manual

ISOLATE II Blood DNA Kit. Product Manual ISOLATE II Blood DNA Kit Product Manual 2 Product Manual www.bioline.com/isolate Blood DNA Kit ISOLATE II Blood DNA Kit ISOLATE II Blood DNA Kit 1 Kit contents 04 2 Description 04 3 Storage 05 4 Safety

More information

Average Yields* Yeast DNA Yeast RNA Time to Complete 10 Purifications * Yield will vary depending on the type of sample processed

Average Yields* Yeast DNA Yeast RNA Time to Complete 10 Purifications * Yield will vary depending on the type of sample processed 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Fungi/Yeast RNA/DNA Purification Kit Product # 35800 Product Insert

More information

Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions

Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions Maxim Biotech, Inc. 780 Dubuque Avenue, So. San Francisco, CA 94080, U.S.A. Tel: (800) 989-6296

More information

Presto Food DNA Extraction Kit

Presto Food DNA Extraction Kit Instruction Manual Ver. 06.28.17 For Research Use Only Presto Food DNA Extraction Kit Advantages FGD004 (4 Preparation Sample Kit) FGD100 (100 Preparation Kit) FGD300 (300 Preparation Kit) Sample: 200

More information

I-Blue Midi Plasmid Kit. I-Blue Midi Plasmid Kit. (Endotoxin Free) IBI SCIENTIFIC. Instruction Manual Ver For Research Use Only.

I-Blue Midi Plasmid Kit. I-Blue Midi Plasmid Kit. (Endotoxin Free) IBI SCIENTIFIC. Instruction Manual Ver For Research Use Only. Instruction Manual Ver. 05.11.17 For Research Use Only I-Blue Midi Plasmid Kit & I-Blue Midi Plasmid Kit (Endotoxin Free) IB47180, IB47190 (2 Preparation Sample Kit) IB47181, IB47191 (25 Preparation Kit)

More information

E.Z.N.A. PF Micro RNA Kit. R preps R preps

E.Z.N.A. PF Micro RNA Kit. R preps R preps E.Z.N.A. PF Micro RNA Kit R7036-00 5 preps R7036-01 50 preps June 2013 E.Z.N.A. PF Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

Kits for isolation of plasmid DNA in medium scale

Kits for isolation of plasmid DNA in medium scale PLASMID MIDI KIT PLASMID MIDI ENDOTOXIN-FREE Cat. No. EM16 / EM17 Version: 1.2014 Kits for isolation of plasmid DNA in medium scale EXTRACTME is a registered trademark of BLIRT S.A. PLASMID MIDI KIT PLASMID

More information

Urine DNA Isolation Maxi Kit (Slurry Format) Product # 50100

Urine DNA Isolation Maxi Kit (Slurry Format) Product # 50100 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation Maxi Kit (Slurry Format) Product # 50100 Product

More information

Geneaid Maxi Plasmid Kit & Geneaid Maxi Plasmid Kit (Endotoxin Free)

Geneaid Maxi Plasmid Kit & Geneaid Maxi Plasmid Kit (Endotoxin Free) Geneaid Maxi Plasmid Kit & Geneaid Maxi Plasmid Kit (Endotoxin Free) PM002, PME02 (2 Preparation Sample Kit) PM010, PME10 (10 Preparation Kit) PM025, PME25 (25 Preparation Kit) Instruction Manual Ver.

More information

INDEX INDEX 0 KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 IMPORTANT NOTES 2 EUROGOLD TOTAL RNA ISOLATION PROTOCOL 2 DNA CONTAMINATION 5

INDEX INDEX 0 KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 IMPORTANT NOTES 2 EUROGOLD TOTAL RNA ISOLATION PROTOCOL 2 DNA CONTAMINATION 5 INDEX INDEX 0 KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 IMPORTANT NOTES 2 EUROGOLD TOTAL RNA ISOLATION PROTOCOL 2 DNA CONTAMINATION 5 QUANTITATION AND STORAGE OF RNA 5 RNA QUALITY 5 TROUBLESHOOTING

More information

MagListo 5M Nucleic Acid Extraction Kit MagListo Magnetic Separation Rack

MagListo 5M Nucleic Acid Extraction Kit MagListo Magnetic Separation Rack Bioneer s magnetic nanobead-based solution for DNA/RNA extraction and purification 20150910 Ver.1(EN) MagListo 5M Nucleic Acid Extraction Kit MagListo Magnetic Separation Rack Fast, easy separation - Just

More information

Plasmid Midiprep Plus Purification Kit. Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only

Plasmid Midiprep Plus Purification Kit. Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only Plasmid Midiprep Plus Purification Kit Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only 1 Description: The Plasmid Midiprep Plus Purification Kit provides simple

More information

96 well Plant Genomic DNA Purification Kit

96 well Plant Genomic DNA Purification Kit 96 well Plant Genomic DNA Purification Kit Cat. #: DP022-9602/ DP022-9608 Size: 2 x 96 / 8 x 96 Reactions Store at RT For Research Use Only 2015.10.08 1 Description : The 96 well Plant Genomic DNA Purification

More information

Spin Micro DNA Extraction Kit

Spin Micro DNA Extraction Kit Spin Micro DNA Extraction Kit 2-020 20 extractions 18-25 C 1. Lysis Buffer (brown cap) 2x 2 ml R 36 2. Binding Buffer (red cap) 3x 2 ml R 11/36/67 3. Protease (orange cap) lyophilized R 36/37/38 Add 250

More information

Note: for laboratory research use only. RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Signalway Biotechnology

Note: for laboratory research use only. RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Signalway Biotechnology Note: for laboratory research use only RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Cat. #: RP1202 (50preps) Signalway Biotechnology I. Kit Content, Storage Condition and Stability Content

More information

Plant Total RNA Purification Kit. Cat. #.: TR02 / TR Size : 50 / 150 Reactions Store at RT For research use only

Plant Total RNA Purification Kit. Cat. #.: TR02 / TR Size : 50 / 150 Reactions Store at RT For research use only Plant Total RNA Purification Kit Cat. #.: TR02 / TR02-150 Size : 50 / 150 Reactions Store at RT For research use only 1 Description: The Plant Total RNA Purification Kit provides a rapid, simple and effective

More information

Product # (12 samples) Solution WN. Elution Buffer B Proteinase K Maxi Spin Columns 12 Collection Tubes 12 Elution Tubes 12 Product Insert 1

Product # (12 samples) Solution WN. Elution Buffer B Proteinase K Maxi Spin Columns 12 Collection Tubes 12 Elution Tubes 12 Product Insert 1 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Blood DNA Isolation Maxi Kit Product # 31200 Product Insert Norgen

More information

LaboPass TM Tissue mini

LaboPass TM Tissue mini LaboPass TM Tissue mini Protocol Book LaboPass TM Tissue Mini Introduction LaboPass TM Tissue Kit provides a simple and fast method for the isolation of total DNA from up to 40 mg of animal tissue or 5

More information

E.Z.N.A. Tissue RNA Kit. R preps R preps

E.Z.N.A. Tissue RNA Kit. R preps R preps E.Z.N.A. Tissue RNA Kit R6688-00 5 preps R6688-01 50 preps May 2015 E.Z.N.A. Tissue RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Important Notes...4 Homogenization

More information

Total Arrest RNA. For Isolation of DNA free RNA for RT PCR. (Cat. # , ) think proteins! think G-Biosciences

Total Arrest RNA. For Isolation of DNA free RNA for RT PCR. (Cat. # , ) think proteins! think G-Biosciences 445PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences. com A Geno Technology, Inc. (USA) brand name Total Arrest RNA For Isolation of DNA free RNA for RT PCR (Cat. #786 130, 786 131)

More information

Human whole blood RNA Purification Kit

Human whole blood RNA Purification Kit Human whole blood RNA Purification Kit Cat. #.: TR03 / TR03-150 Size : 50 / 150 Reactions Store at RT For research use only 2016.01.11 1 Description: The Human Blood RNA Purification Kit provides a rapid,

More information

Plasmid DNA Extraction Midiprep Kit

Plasmid DNA Extraction Midiprep Kit BIO BASIC Plasmid DNA Extraction Midiprep Kit 9K-006-0027s (1 prep) 9K-006-0027 (25 preps) Toll Free 1-877-956-7778 ForOrder Research Use Only www.feldan.com 1 Index Plasmid DNA Extraction Midiprep Kit

More information

Cytoplasmic & Nuclear RNA Purification Kit Product # 21000, 37400

Cytoplasmic & Nuclear RNA Purification Kit Product # 21000, 37400 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cytoplasmic & Nuclear RNA Purification Kit Product # 21000, 37400

More information

Mag-Bind Blood DNA KF 96 Kit. M x 96 preps M x 96 preps

Mag-Bind Blood DNA KF 96 Kit. M x 96 preps M x 96 preps Mag-Bind Blood DNA KF 96 Kit M6321-00 1 x 96 preps M6321-01 4 x 96 preps June 2013 Mag-Bind Blood DNA KF 96 Kit Table of Contents Introduction and Principle...2 Kit Contents and Storage...3 Preparing Reagents...4

More information

Mag-Bind PX Blood RNA 96 Kit. M x 96 preps M x 96 preps

Mag-Bind PX Blood RNA 96 Kit. M x 96 preps M x 96 preps Mag-Bind PX Blood RNA 96 Kit M7763-00 1 x 96 preps M7763-01 4 x 96 preps June 2018 Mag-Bind PX Blood RNA 96 Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Plasmid Midiprep Purification Kit

Plasmid Midiprep Purification Kit Plasmid Midiprep Purification Kit Cat. # : DP01MD/ DP01MD-100 Size : 20/100 Reactions Store at RT For research use only 1 Description: The Plasmid Midiprep Purification Kit provides simple rapid protocol

More information

Plasmid Maxiprep Plus Purification Kit

Plasmid Maxiprep Plus Purification Kit Plasmid Maxiprep Plus Purification Kit Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only 1 Description: The Plasmid Maxiprep Plus Purification Kit provides simple,

More information

Urine DNA Isolation Kit for Exfoliated Cells or Bacteria Product # 47050

Urine DNA Isolation Kit for Exfoliated Cells or Bacteria Product # 47050 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation Kit for Exfoliated Cells or Bacteria Product

More information

Plant/Fungi Total RNA Purification Kit Product # 25800, 31350, 25850

Plant/Fungi Total RNA Purification Kit Product # 25800, 31350, 25850 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plant/Fungi Total RNA Purification Kit Product # 25800, 31350,

More information

2 x 0.5ml. STORAGE CONDITIONS The kit is shipped at ambient temperature. Upon arrival, store the kit components as recommended on the reagent label.

2 x 0.5ml. STORAGE CONDITIONS The kit is shipped at ambient temperature. Upon arrival, store the kit components as recommended on the reagent label. 305PR 01 G-Biosciences, St Louis, MO. USA 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name OmniPrep For High Quality Genomic DNA Extraction INTRODUCTION

More information

High Pure RNA Isolation Kit for isolation of total RNA from 50 samples Cat. No

High Pure RNA Isolation Kit for isolation of total RNA from 50 samples Cat. No for isolation of total RNA from 50 samples Cat. No. 1 88 665 Principle A single reagent lyses the sample lysis and inactivates RNase. In the presence of a chaotropic salt (guanidine HCl), the released

More information

Lysis Buffer A Wash Solution A Elution Buffer B Mini Filter Spin Columns 50 Collection Tubes 50 Elution tubes (1.7 ml) 100 Product Insert 1

Lysis Buffer A Wash Solution A Elution Buffer B Mini Filter Spin Columns 50 Collection Tubes 50 Elution tubes (1.7 ml) 100 Product Insert 1 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine DNA Isolation Kit (Slurry Format) Product # 48800 Product

More information

Page 2 of 16. Introduction. Contents

Page 2 of 16. Introduction. Contents Contents Introduction... 2 Storage and Stability... 2 Kit Contents... 3 Before Starting... 3 A. SQ Blood DNA Mini Protocol for 100-400ul blood... 4 B. SQ Blood DNA Midi Protocol for 500ul-3ml blood...

More information

TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0

TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 Cat. # 9760 For Research Use TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 Product Manual Table of Contents I. Description... 3 II. Kit Components... 3 III. Shipping and Storage... 4 IV. Preparation

More information

Kits for isolation of plasmid DNA in large scale

Kits for isolation of plasmid DNA in large scale PLASMID MAXI KIT PLASMID MAXI ENDOTOXIN-FREE Cat. No. EM18 / EM19 Version: 1.2014 Kits for isolation of plasmid DNA in large scale EXTRACTME is a registered trademark of BLIRT S.A. PLASMID MAXI KIT PLASMID

More information

Thermo Scientific GeneJET Whole Blood RNA Purification Mini Kit #K0761

Thermo Scientific GeneJET Whole Blood RNA Purification Mini Kit #K0761 PRODUCT INFORMATION Thermo Scientific GeneJET Whole Blood RNA Purification Mini Kit #K0761 www.thermoscientific.com/onebio #K0761 Lot Exp. CERTIFICATE OF ANALYSIS Thermo Scientific GeneJET Whole Blood

More information

Plasmid Maxiprep Plus Purification Kit. Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only

Plasmid Maxiprep Plus Purification Kit. Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only Plasmid Maxiprep Plus Purification Kit Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only 1 Description: The Plasmid Maxiprep Plus Purification Kit provides simple,

More information

GRS Genomic DNA Kit BroadRange - #GK

GRS Genomic DNA Kit BroadRange - #GK revised: 18 05 2012 printed: 21 05 2012 GRS Genomic DNA Kit BroadRange - #GK06.0100 (FOR RESEARCH ONLY) Sample : up to 200µl of whole blood (fresh or frozen), up to 25 mg of tissue, up to 25mg of paraffinembedded

More information

Plant microrna Purification Kit Product # 54700

Plant microrna Purification Kit Product # 54700 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plant microrna Purification Kit Product # 54700 Product Insert

More information

mdi Stool Genomic DNA Miniprep Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)

mdi Stool Genomic DNA Miniprep Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA) Stool Genomic DNA Miniprep Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax:

More information

PureLink Plant Total DNA Purification Kit

PureLink Plant Total DNA Purification Kit USER GUIDE PureLink Plant Total DNA Purification Kit For purification of DNA from plant Catalog Number K1830-01 Document Part Number 25-0757 Publication Number MAN0000478 Revision 2.0 For Research Use

More information

Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).

Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate). Page 0 INSTRUCTION MANUAL Quick-DNA Miniprep Kit Catalog Nos. D3024 & D3025 Highlights Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs

More information